Adult ovine connective tissue cells resemble mesenchymal stromal cells in their propensity for extensive ex vivo expansion.
Conclusions: Cells from ovine connective tissues resembled MSCs in their propensity for sustained, colony-forming growth and expression of CD molecules, which supports the potential for preclinical testing of MSC-like connective tissue cells in sheep. PMID: 33153311 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - November 11, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Comparison between placental and skeletal muscle ECM: in vivo implantation.
CONCLUSIONS: These results demonstrated that xenogeneic placental ECM, implanted heterotopically (representing a biologically critical and challenging microenvironment), induced local inflammatory reactions similar to the allogeneic muscle ECM, implanted orthotopically. Thus, placenta-derived extracellular matrix must be further explored in regenerative medicine. PMID: 33106052 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 28, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Induced Hypothyroidism Alters Articular Cartilage in Skeletally Immature Miniature Swine.
CONCLUSIONS: The significant changes in articular cartilage structure in hypothyroid compared to control immature miniature swine suggest that thyroid hormone is critical in the growth and development of articular cartilage. CLINICAL SIGNIFICANCE: Understanding articular cartilage development in immature animal models may provide insight into healing or repair of degenerative human articular cartilage. PMID: 33073630 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 21, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

A retrotransposon gag-like-3 gene RTL3 and SOX-9 co-regulate the expression of COL2A1 in chondrocytes.
CONCLUSIONS: Our results identify a novel regulatory mechanism of COL2A1 expression in chondrocytes that may help to further understand the skeletal development and the pathogenesis of diseases with altered COL2A1 expression. PMID: 33043724 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 14, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Increase in molar mass distinguishes chondroitin sulfate from osteoarthritis and normal extracellular cartilage matrix.
Authors: Nunes RM, Girão VCC, Castro RR, Cunha PLR, Feitosa JPA, Pinto ACMD, Castro Rocha FA Abstract PURPOSE: To determine alterations of chondroitin sulfate (CS) that reflect cartilage damage in an experimental osteoarthritis (OA) model as well as in human OA samples. MATERIALS AND METHODS: Rats were subjected to anterior cruciate ligament transection (ACLT; OA) or a sham procedure and sacrificed 14, 28, or 70 days after ACLT for histopathology and analysis of extracted CS. Cartilage samples from 14 patients undergoing hip or shoulder arthroplasty secondary to OA or fracture (control) were subject...
Source: Connective Tissue Research - October 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Spatial frequency metrics for analysis of microscopic images of musculoskeletal tissues.
Conclusions: Our results indicate that these metrics can quantify musculoskeletal tissues during development and regeneration. PMID: 33028134 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles.
CONCLUSIONS: Our results suggest that there is steady-state transcription of fibrogenic genes in muscles with established fibrosis, implying that post-transcriptional processes are responsible for the increased protein levels of fibrotic factors during muscle overuse conditions. We hypothesize that targeting such pathways represents a valid approach to treat overuse injury. Alternatively, FGF2 gene expression may represent a valid target for therapy. PMID: 33030055 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

LncRNA SNHG5 promotes chondrocyte proliferation and inhibits apoptosis in osteoarthritis by regulating miR-10a-5p/H3F3B axis.
CONCLUSION: SNHG5 suppressed chondrocytes apoptosis in OA by regulating the miR-10a-5p/H3F3B axis, which provided a promising biomarker for OA treatment. PMID: 32967481 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 26, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Minoxidil decreases collagen I deposition and tissue-like contraction in clubfoot-derived cells: a way to improve conservative treatment of relapsed clubfoot?
CONCLUSIONS: Minoxidil exerts an in vitro inhibitory effect on the cell proliferation, collagen accumulation, and extracellular matrix contraction processes that are associated with clubfoot fibrosis. This study provides important preliminary results demonstrating the potential relevance of MXD for adjuvant pharmacological therapy in standard treatment of relapsed clubfoot. PMID: 32951485 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 22, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Tendon cells derived from the long head of the biceps and the supraspinatus tendons of patients affected by rotator cuff tears show different expressions of inflammatory markers.
CONCLUSION: SSP-TCs showed higher cellular stress and expression of inflammatory markers with respect to donor-matched LHB-TCs, suggesting that addressing the physio-pathological state of supraspinatus tendon cells during treatment of rotator cuff tears could favor tissue healing and possibly prevent relapses. PMID: 32921180 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 16, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Responses of matrix metalloproteinases to hyperbaric oxygen treatment: changing for good or ill?
Authors: Silva FS, Canêdo VSR, Abreu BJ, Oliveira MF Abstract Hyperbaric oxygen (HBO2) is currently emerging as a promising therapeutic option for diseases involving impaired tissue repair and remodeling. In this regard, HBO2 has been shown to modulate signaling pathways responsible for matrix metalloproteinases (MMPs) regulation, which makes the MMPs interesting targets for investigation. However, the understanding regarding how HBO2 treatment affects the expression and activity of the MMP family members in different tissues and diseases needs to be clarified. The precise roles of MMPs in the physiopathology...
Source: Connective Tissue Research - September 11, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Potential cross-talk between muscle and tendon in Duchenne muscular dystrophy.
CONCLUSIONS: Duchenne muscular dystrophy results from the absence of the protein dystrophin and the associated dystrophin - glycoprotein complex, which are thought to provide both structural support and signaling functions for the muscle fiber. In addition, there are other potential signal pathways that could represent cross-talk between muscle and tendon, particularly at the myotendinous junction. Duchenne muscular dystrophy is characterized by multiple pathophysiologic mechanisms. Herein, we explore three of these: (1) the extracellular matrix, fibrosis, and fat deposition; (2) satellite cells; and (3) tensegrity. A key ...
Source: Connective Tissue Research - September 2, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Diversity in heritable disorders of connective tissue at a single center.
CONCLUSION: A wide diversity in HDCT was observed at a single center. Therefore, knowledge about the phenotype-genotype correlation in HDCT is still crucial in the diagnosis of this group of diseases, and an improvement in the screening tool will be needed. PMID: 32862725 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 1, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Extracellular matrix at the muscle - tendon interface: functional roles, techniques to explore and implications for regenerative medicine.
Authors: Narayanan N, Calve S Abstract The muscle-tendon interface is an anatomically specialized region that is involved in the efficient transmission of force from muscle to tendon. Due to constant exposure to loading, the interface is susceptible to injury. Current treatment methods do not meet the socioeconomic demands of reduced recovery time without compromising the risk of reinjury, requiring the need for developing alternative strategies. The extracellular matrix (ECM) present in muscle, tendon, and at the interface of these tissues consists of unique molecules that play significant roles in homeostasis and...
Source: Connective Tissue Research - August 30, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Contrast-enhanced x-ray microscopy of articular cartilage.
This study evaluates articular cartilage structure ex vivo using both techniques. Osteochondral plugs were excised from non-diseased bovine knees and stained in phosphotungstic acid for 0 to 32 hours. XRM imaging revealed an optimal staining time of 16 hrs and a saturated staining time of 24 hrs. Histology sections were cut and analysed by polarized light microscopy (PLM) and second-harmonic-generation dual-photon (SHG-DP) microscopy. Histology photomicrographs were aligned with matching XRM slices, and evaluated for features relevant in histopathological scoring of osteoarthritis cartilage, including the tidemark, collage...
Source: Connective Tissue Research - August 22, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Optogenetic activation of muscle contraction in vivo.
CONCLUSIONS:  Our results demonstrate the feasibility and repeatability of non-invasive optogenetic stimulation of muscle in vivo and highlight optogenetic stimulation as a powerful tool for non-invasive in vivo direct activation of skeletal muscle. PMID: 32777957 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - August 13, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Blocking CCN2 preferentially inhibits osteoclastogenesis induced by repetitive high force bone loading.
CONCLUSIONS: HRHF loading increased bone formation in each task group, yet blocking CCN2 dampened trabecular bone catabolism by reducing osteoclast numbers and activity. PMID: 32683988 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - July 21, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Identification of key potential targets for TNF- α/TNFR1-related intervertebral disc degeneration by bioinformatics analysis.
Identification of key potential targets for TNF-α/TNFR1-related intervertebral disc degeneration by bioinformatics analysis. Connect Tissue Res. 2020 Jul 19;: Authors: Hong J, Yan J, Chen J, Li S, Huang Y, Huang Z, Chen W, Liang A, Ye W Abstract BACKGROUND: Bioinformatics analysis was performed on gene expression profile microarray data to identify the key genes activated through the TNF-α/TNFR1 signaling pathway in intervertebral disc degeneration (IDD). The common differentially expressed genes (co-DEGs) were calculated in nucleus pulposus (NP) cells and annulus fibrosus (AF) cells under ...
Source: Connective Tissue Research - July 21, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Multiomics analysis of the mdx/mTR mouse model of Duchenne muscular dystrophy.
CONCLUSIONS: These findings highlight the utility of multiomics in studying muscle disease, and provide additional insight into the pathological changes in dystrophic muscles that might help to indirectly guide evidence-based nutritional or exercise prescription in DMD patients. PMID: 32664808 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - July 17, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Volumetric muscle loss disrupts length-dependent architectural and functional characteristics of skeletal muscle.
Authors: Goldman SM, Feng JP, Corona BT Abstract PURPOSE/AIM: Skeletal muscle architecture is a primary determinant of function. Volumetric muscle loss (VML) injury is destructive; however, the impact on muscle architecture is uncharacterized. METHODS: Architectural and functional effects of VML were assessed in rat tibialis anterior (TA) muscle model 4 weeks post-injury. RESULTS: VML caused a 31% and 33% reduction in muscle weight (p
Source: Connective Tissue Research - July 16, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Basement membrane recovery process in rat soleus muscle after exercise-induced muscle injury.
CONCLUSIONS: DR induces BM disruption accompanied by muscle fiber damage. It is possible that BM destruction may be accompanied by muscle damage and that TCF4 (+) cells contribute to muscle fiber and BM recovery. PMID: 32619127 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - July 4, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Magnolin exhibits anti-inflammatory effects on chondrocytes via the NF- κB pathway for attenuating anterior cruciate ligament transection-induced osteoarthritis.
CONCLUSION: MGL demonstrated significant anti-inflammatory bioactivity on chondrocytes by suppressing the activation of NF-κB pathway, which in turn exhibited a significant alleviation of OA. PMID: 32602381 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - July 1, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Therapeutic application of extracellular vesicles for musculoskeletal repair & regeneration.
Therapeutic application of extracellular vesicles for musculoskeletal repair & regeneration. Connect Tissue Res. 2020 Jun 30;:1-16 Authors: Youssef El Baradie KB, Hamrick MW Abstract Traumatic musculoskeletal injuries are common in both the civilian and combat care settings. Significant barriers exist to repairing these injuries including fracture nonunion, muscle fibrosis, re-innervation, and compartment syndrome, as well as infection and inflammation. Recently, extracellular vesicles (EVs), including exosomes and microvesicles, have attracted attention in the field of musculoskeletal regeneration...
Source: Connective Tissue Research - July 1, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Pleiotropin enhances the osteo/dentinogenic differentiation potential of dental pulp stem cells.
CONCLUSION: Our research demonstrated that PTN has a positive role in maintaining DPSCs proliferation and osteo/dentinogenic differentiation potential. PMID: 32580608 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 27, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Regenerative rehabilitation of catastrophic extremity injury in military conflicts and a review of recent developmental efforts.
CONCLUSIONS: Both technological improvements and a rapidly advancing understanding of injury pathophysiology will contribute to improved limb-salvage outcomes, and shift the calculus away from early limb amputation. PMID: 32552156 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 20, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Maresin-1 suppresses IL-1 β-induced MMP-13 secretion by activating the PI3K/AKT pathway and inhibiting the NF-κB pathway in synovioblasts of an osteoarthritis rat model with treadmill exercise.
CONCLUSIONS: The changes in maresin-1 in IALF, as seen in our single-section treadmill exercise, provides an explanation for the therapeutic effect of appropriate-strength treadmill exercise on osteoarthritis, and our experiments confirmed the therapeutic effect of maresin-1 both in vivo and in vitro. PMID: 32546009 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 19, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Naringin inhibits titanium particles-induced up-regulation of TNF- α and IL-6 via the p38 MAPK pathway in fibroblasts from hip periprosthetic membrane.
CONCLUSIONS: These results indicated that naringin could inhibit Ti particle-induced inflammation in fibroblasts by inhibiting p38 MAPK/NF-κB p65 activity and might be a potential drug for the treatment of inflammatory periprosthetic osteolysis after arthroplasty. PMID: 32500755 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 7, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Overexpression of laminin α4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling.
CONCLUSIONS: LAMA4 positively regulated cellular proliferation and migration in fibroblasts via activating the Shh/Gli1 signaling. LAMA4/Shh/Gli1 signaling axis might be a potential therapeutic target for the prevention of surgery-induced intraarticular fibrosis. PMID: 32449381 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 27, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Morphology and Serum and Bone Tissue Calcium and Magnesium Concentrations in the Bones of Male Rats Chronically Treated with Letrozole, a Nonsteroidal Cytochrome P450 Aromatase Inhibitor.
CONCLUSION: Chronic treatment with letrozole affected cortical bone structure and produced histomorphological changes in male rat bone similar to that observed in the aging processes. PMID: 32419518 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 19, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Comparison of the efficiency of laminin versus fibronectin as a differential adhesion assay for isolation of human articular cartilage derived chondroprogenitors.
Conclusion: This was the first attempt to successfully isolate human articular cartilage derived chondroprogenitor clones using laminin, which retained stem cell like characteristics. Further evaluation to optimize this method will help enhance chondroprogenitor characteristics, for use in cartilage repair. PMID: 32406271 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 15, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Induced neural stem cells-derived spinal cord progenitor cells as stable stage for rapid and efficient generation of human oligodendrocytes and motor neurons.
Conclusion: Our research provides a useful protocol for rapid generation of human oligodendrocytes and motor neurons from human iNSCs and demonstrates a progenitor cell model for exploring the origin of motor neurons and oligodendrocyte in vitro, which will contribute to research on the development of spinal cord and regenerative medicine. PMID: 32380866 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

RANKL expression of primary osteoblasts is enhanced by an IL-17-mediated JAK2/STAT3 pathway through autophagy suppression.
Conclusion: Overall, our findings demonstrate, for the first time, that IL-17 modulates RANKL expression of osteoblasts through an autophagy-JAK2-STAT3 signaling pathway, thus affecting bone metabolism. PMID: 32370570 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 8, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Methods for studying MLO-Y4 osteocytes in collagen-hydroxyapatite scaffolds in the rotary cell culture system.
Authors: Fournier R, Harrison RE Abstract Purpose: The rotary cell culture system (RCCS) is a common clinorotation device for cell culture. It is also used as a low-shear suspension culture bioreactor to form functionalized 3D tissue constructs and to model microgravity. We sought to develop a 3D scaffold composed of type I collagen and hydroxyapatite (collagen-HA) to characterize MLO-Y4 osteocytes following suspension culture or clinorotation.Materials and Methods: MLO-Y4 cells were embedded in collagen-HA. The scaffold was formed into droplets for suspension culture or wall-adhered to the RCCS for clinorotation. ...
Source: Connective Tissue Research - May 8, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Ex vivo models of musculoskeletal tissues.
Authors: Szczesny SE PMID: 32340565 [PubMed - in process] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 29, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Downregulation of lncRNA XIST promotes proliferation and differentiation, limits apoptosis of osteoblasts through regulating miR-203-3p/ZFPM2 axis.
Conclusion: Downregulation of lncRNA XIST promoted osteoblast proliferation and differentiation, but limited apoptosis by miR-203-3p/ZFPM2 axis. PMID: 32326773 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 25, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care. Proteoglycan-4 concentrations were measured by amplified-luminescence-proximity-assay and enzyme-linked-immunosorbent-assay in SF and serum, respectively. Molecular-weight of hyaluronan was characterized by agarose-gel-electrophoresis, and concentrations were measured by enzyme-linked-immunosorbent-assay ...
Source: Connective Tissue Research - April 21, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Comparison of Dynamic Mechanical Properties of Dentin Between Deciduous and Permanent Teeth.
The objective of the present study was to quantify the differences between deciduous and permanent dentin under cyclic mechanical loading, which is similar to masticatory stress.Materials and Methods: Deciduous and permanent teeth, respectively from children (9~12 years old) and young people (18~25 years old), were wet-sectioned perpendicular to the longitudinal axis and the central specimens of coronal dentin were evaluated by nanoscopic dynamic mechanical analysis (nanoDMA).Results: The average storage, loss, and complex moduli, as well as the hardness of deciduous dentin were significantly (p
Source: Connective Tissue Research - April 21, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

HOXA10 may inhibit the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin and DKK1.
CONCLUSION: These data demonstrate that HOXA10 inhibits the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin localization and DKK1. PMID: 32299243 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 18, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Compression stress induces nucleus pulposus cell autophagy by inhibition of the PI3K/AKT/mTOR pathway and activation of the JNK pathway.
Conclusions: These results show a new regulatory mechanism involving ROS-mediated autophagy in rat NP cells, which may provide ideas for drug development to improve compression stress-induced IVDD and help avoid eventual surgical treatment of IVD herniation. PMID: 32180463 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 19, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

PBN protects NP cells from AAPH-induced degenerative changes by inhibiting the ERK1/2 pathway.
Conclusions: Our study for the first time identified a novel role and mechanism for PBN in protecting the IVD against oxidative stress, matrix catabolism and apoptosis, which may have implications for its further application in combating IVD degenerative diseases. PMID: 32183547 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 19, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Ontogeny informs regeneration: explant models to investigate the role of the extracellular matrix in cartilage tissue assembly and development.
The objective of this review is to highlight methods that investigate cartilage ontogenesis with native and model systems in the context of regenerative medicine. Specific emphasis is placed on the use of cartilage explant cultures that provide a physiologically relevant microenvironment to study tissue assembly and development. Ex vivo cartilage has proven to be a cost-effective and accessible model system that allows researchers to control the culture conditions and stimuli and perform proteomics and imaging studies that are not easily possible using in vivo experiments, while preserving native cell-matrix interactions. ...
Source: Connective Tissue Research - March 19, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

GREM1 inhibits osteogenic differentiation, senescence and BMP transcription of adipose-derived stem cells.
Conclusions: In summary, our findings suggested that GREM1 inhibited ADSCs senescence and osteogenic differentiation and antagonized BMP transcription. PMID: 32151168 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 12, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Advances in bioprinting: a toolbox for tissue engineering.
Authors: Gottardi R PMID: 32081098 [PubMed - in process] (Source: Connective Tissue Research)
Source: Connective Tissue Research - February 22, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Evidence that reduction in volume protects in situ articular chondrocytes from mechanical impact.
Authors: Kotelsky A, Carrier JS, Aggouras A, Richards MS, Buckley MR Abstract Chondrocytes, the resident cells in articular cartilage, carry the burden of producing and maintaining the extracellular matrix (ECM). However, as these cells have a low proliferative capacity and are not readily replaced, chondrocyte death due to extreme forces may contribute to the pathogenesis of osteoarthritis (OA) after injury or may inhibit healing after osteochondral transplantation, a restorative procedure for damaged cartilage that requires a series of mechanical impacts to insert the graft. Consequently, there is a need to under...
Source: Connective Tissue Research - January 16, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Tendon explant models for physiologically relevant in vitro study of tissue biology - a perspective.
Authors: Wunderli SL, Blache U, Snedeker JG Abstract Background: Tendon disorders increasingly afflict our aging society but we lack the scientific understanding to clinically address them. Clinically relevant models of tendon disease are urgently needed as established small animal models of tendinopathy fail to capture essential aspects of the disease. Two-dimensional and three-dimensional cell and tissue culture models are similarly limited, lacking many physiological extracellular matrix cues required to maintain tissue homeostasis or guide matrix remodeling. These cues reflect the biochemical and biomechanical ...
Source: Connective Tissue Research - January 15, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Mechanosensitive Ca2+ signaling and coordination is diminished in osteocytes of aged mice during ex vivo tibial loading.
Conclusions: We have demonstrated, for the first time, real-time evidence of the diminished mechanosensing and lack of signaling coordination in aged osteocyte networks in tibial explants, which may contribute to pathology of age-induced bone loss. PMID: 31931640 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 15, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Detrimental effects of long sedentary bouts on the biomechanical response of cartilage to sliding.
Conclusions: These results demonstrated that the regularity of ex vivo activity regimens, specifically the duration of sedentary bouts, had a dominant effect on the biomechanical functions of articular cartilage. In more practical terms, the results suggest that brief but regular movement patterns (e.g., every hour) may be biomechanically preferred to long and infrequent movement patterns (e.g., a long walk after a sedentary day) when controlling for daily activity volume (e.g., 30 min). PMID: 31910694 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Authors' answer to a letter to the editor.
Authors: Berteau JP PMID: 31913704 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Letter to the editor.
LETTER TO THE EDITOR. Connect Tissue Res. 2020 Jan 08;:1-2 Authors: Unal M PMID: 31913709 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research

Editorial Note.
Authors: PMID: 31913717 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 9, 2020 Category: Research Tags: Connect Tissue Res Source Type: research