Comparative analysis of mesenchymal stromal cells derived from rabbit bone marrow and Wharton's jelly for adipose tissue engineering.
In conclusion, adipogenic differentiation increased the immunogenicity of both BMSCs and WJ-MSCs, and dedifferentiation inversely decreased their immunogenicity. Adipogenic WJ-MSCs showed significantly higher proliferative and immunoprivileged capacities than BMSCs, and the dedifferentiated BMSCs showed almost the same adipogenic capacity as WJ-MSCs. WJ-MSCs were more suitable than BMSCs for adipose tissue engineering. PMID: 31185754 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 14, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

A Histological Study of the Medial Meniscus Posterior Root Tibial Insertion.
Conclusions: Anatomical insertion of the posterior root of the medial meniscus was located at the sagittal plane passing through the peak of the medial intercondylar tubercle. The structure of the medial meniscus posterior insertion was mainly localized in the anterior one-third. PMID: 31181971 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 12, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Mechanistic complexities of bone loss in Alzheimer's disease: a review.
Conclusions: Skeletal deficits emerge very early in clinical Alzheimer's progression, and cannot be explained by coincident factors such as aging, female sex, mobility status, falls, or genetics. While research in this area is still in its infancy, studies implicate several potential mechanisms in disrupting skeletal homeostasis that include direct effects of amyloid-beta pathology on bone cells, neurofibrillary tau-induced damage to neural centers regulating skeletal remodeling, and/or systemic Wnt/Beta-catenin signaling deficits. Data from an increasing number of studies substantiate a role for the newly discovered "...
Source: Connective Tissue Research - June 12, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Microarray analysis of cartilage: comparison between damaged and non-weight-bearing healthy cartilage.
Conclusion: The genes examined in this study differ distinctly between focal cartilage (ICRS 3-4) lesions and undamaged sites of the same joint. We believe that the data set forth in this study may be used for clinical purposes and be a guide in the development of new biological approaches for therapy. PMID: 31142155 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - June 1, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Differential expression of adipokines in the synovium and infrapatellar fat pad of osteoarthritis patients with and without metabolic syndrome.
Authors: Liu B, Gao YH, Dong N, Zhao CW, Huang YF, Liu JG, Qi X Abstract Objective: To evaluate the expression levels of adipokines in the synovium and infrapatellar fat pad (IPFP) in osteoarthritis (OA) patients with and without metabolic syndrome (MetS). Methods: 120 female patients with OA were enrolled, and 60 healthy women matched body mass index, age, and sex, served as controls. Adipokines levels were measured using a sandwich enzyme-linked immunosorbent assay of the serum of all participants and synovial fluid (SF) of OA patients. Local expression levels of adipokines in the synovium and IPFP were examined ...
Source: Connective Tissue Research - May 31, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Regional differences in turnover, composition, and mechanics of the porcine flexor tendon.
Authors: Zhang C, Svensson RB, Couppé C, Schjerling P, Skovgaard D, Kjaer M, Magnusson SP Abstract Purpose: Recent data suggest that there is a lack of turnover in the core of human tendon, but it remains unknown whether there are regional differences between core and periphery of the cross section. The purpose of this project was to investigate regional differences in turnover as estimated by the accumulation of fluorescent Advanced Glycation End-products (AGEs) and regional differences in mechanical properties. Materials and methods: Tendons were obtained from lean control (n = 4) and diabetic Götting...
Source: Connective Tissue Research - May 29, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Cartilage tissue engineering combining microspheroid building blocks and microneedle arrays.
Conclusions: We constructed defined scaffold-free shapes by bioprinting and fusing microspheroids. Proof of concept was shown in the repair of ex vivo osteoarthritic human cartilage and in vivo rabbit osteochondral (OC) defects. PMID: 31134817 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 29, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

The effect of intervertebral disc degenerative change on biological characteristics of nucleus pulposus mesenchymal stem cell: an in vitro study in rats.
Conclusions: N-NPMSC showed significantly higher proliferation rate, better colony forming and stemness maintenance ability, whereas reduced cell apoptosis rate compared with D-NPMSC. HIF-1α-mediated signal pathway may be involved in the regulation of NPMSC proliferation. These findings indicated that degenerative change of IVD should be taken into account when selecting a source of NPMSC for clinical application. PMID: 31119993 [PubMed - in process] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 25, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Triceps surae muscle-subtendon interaction differs between young and older adults.
Conclusions: These findings provide an important biomechanical basis for previously reported correlations between more uniform Achilles subtendon behavior and reduced ankle moment generation during waking in older adults. PMID: 31116576 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 23, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Depletion of PRDM9 enhances proliferation, migration and chemotaxis potentials in human periodontal ligament stem cells.
CONCLUSION: These discoveries indicated that depletion of PRDM9 increased the cell proliferation, migration and chemotaxis potential of PDLSCs and revealed important downstream genes. PMID: 31096797 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 19, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Histone demethylase Jmjd3 modulates osteoblast apoptosis induced by tumor necrosis factor-alpha through directly targeting RASSF5.
CONCLUSIONS: Jmjd3 regulated TNF-α-induced osteoblast apoptosis by targeting RASSF5. PMID: 31092054 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 18, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Extensive burn injury causes bone collagen network alteration and growth delay related to RANK-L immunoexpression change.
This study aimed to investigate bone histopathological and morphometric aspects, collagen fibers network and the immunoexpression of biological markers related to bone development in a young experimental model for extensive burn. Twenty-eight male Wistar rats were distributed into: Control (C) and subjected to scald burn injury (SBI) groups. Sham or injured animals were euthanized 4 or 14 days post lesion and proximal epiphyses of the femur were submitted to histological, morphometric (thickness epiphyseal plate) and RUNX-2 and RANK-L immunoexpression methods. Histophatological femoral findings showed delayed appearance of...
Source: Connective Tissue Research - May 18, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Hypoxia reduces the osteogenic differentiation of peripheral blood mesenchymal stem cells by upregulating Notch-1 expression.
CONCLUSIONS: Hypoxia accelerated proliferation, increased migration, and reduced PBMSC differentiation into osteoblasts by increasing Notch1 expression. These findings may contribute to the development of appropriate cell culture or in vivo transplantation conditions to maintain the full osteogenic potential of PBMSCs. PMID: 31035811 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - May 2, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Correction.
Authors: PMID: 31023090 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 28, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Salvianolic acid B combined with mesenchymal stem cells contributes to nucleus pulposus regeneration.
Authors: Yan HS, Hang C, Chen SW, Wang KK, Bo P Abstract PURPOSE: To investigate whether salvianolic acid B is able to enhance repair of degenerated intervertebral discs by mesenchymal stem cells (MSCs) through promotion of MSC differentiation into nucleus pulposus cells in a nucleus-pulposus-like environment and by enhancing the trophic effect of MSCs on residual nucleus pulposus cells (mediated by transforming growth factor-β1). MATERIALS AND METHODS: Successful intervertebral disc degeneration models, established by aspiration of the nucleus pulposus in New Zealand white rabbits, were randomly divided i...
Source: Connective Tissue Research - April 28, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Donor-Matched Comparison of Chondrogenic Progenitors Resident in Human Infrapatellar Fat pad, Synovium and Periosteum - Implications for Cartilage Repair.
This study focuses on comparison of cells and connective tissue progenitors (CTPs) derived from native human infrapatellar fatpad (IPFP), synovium (SYN), and periosteum (PERI). DESIGN: IPFP, SYN, PERI were harvested from twenty-eight patients undergoing arthroplasty. CTPs were quantitatively characterized using automated colony-forming-unit assay to compare total nucleated cell concentration-[Cell], cells/mg; prevalence-(PCTP), CTPs/million nucleated cells; CTP concentration-[CTP], CTPs/mg; proliferation and differentiation potential; and correlate outcomes with patient's age and gender. RESULTS: [Cell] did not dif...
Source: Connective Tissue Research - April 26, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.
Authors: Grier WK, Sun Han Chang RA, Ramsey MD, Harley BAC Abstract BACKGROUND: Orthopedic injuries often occur at the interface between soft tissues and bone. The tendon-bone junction (TBJ) is a classic example of such an interface. Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes. The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the n...
Source: Connective Tissue Research - April 23, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

TSP1 and TSP2 deficiencies affect LOX protein distribution in the femoral diaphysis and pro-peptide removal in marrow-derived mesenchymal stem cells in vitro.
We examined detergent insoluble (mature cross-linked) and soluble (newly secreted) ECM fractions prepared from diaphyseal cortical bone. Detergent-insoluble hydroxyproline content, an indicator of cross-linked collagen content and LOX function, was reduced in female TSP-deficient bones. In male diaphyses, only TSP2 deficiency affected insoluble hydroxyproline content. Western blot suggested that removal of the LOX-pro-peptide (LOPP), an indication of LOX activation, was not affected by TSP status. Instead, the distribution of pro-LOX and mature LOX between immature and mature ECM was altered by TSP-status. LOX was also exa...
Source: Connective Tissue Research - April 5, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

HIF-1 α-TWIST pathway restrains cyclic mechanical stretch-induced osteogenic differentiation of bone marrow mesenchymal stem cells.
CONCLUSIONS: The HIF-1α-TWIST signaling pathway inhibits cyclic mechanical stretch-induced osteogenic differentiation of BMSCs. This finding may facilitate cell and tissue engineering for clinical applications. PMID: 30938209 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 3, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Effects of proteasome inhibitors on cytokines, metalloproteinases and their inhibitors and collagen type-I expression in periprosthetic tissues and fibroblasts from loose arthroplasty endoprostheses.
CONCLUSIONS: These findings suggest that proteasome impairment could be a contributing factor to aseptic loosening. Protection and enhancement of proteasome efficacy could thus be considered as an alternative strategy toward disease treatment. PMID: 30931650 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 2, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Blockade of the OGF-OGFr pathway in diabetic bone.
CONCLUSIONS: The OGF-OGFr pathway appears to be dysregulated in the bone of T1D rats. Topical NTX treatment of T1D fractured bone accelerated some aspects of delayed diabetic fracture repair, and systemic NTX protected against some elements of compromised bone composition. PMID: 30931654 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - April 2, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Raman spectroscopy investigation shows that mineral maturity is greater in CD-1 than in C57BL/6 mice distal femurs after sexual maturity.
This study sought to investigate the main compositional properties of bone tissue between non-pathological C57BL/6J and CD-1 murine knee joints. To this end, medial and lateral condylar subchondral bones and the adjacent diaphyseal cortical bone of 13 murine femurs (n=7 C57BL/6J and n=6 CD-1 at eight weeks old, just after sexual maturation) were analyzed with ex vivo Raman spectroscopy. Regardless of the bone tissue analyzed, our results showed that CD-1 laboratory mice present a more mature mineral phase than C57BL/6J laboratory mice, but present no difference in maturity of collagen phase. For both strains, the subchondr...
Source: Connective Tissue Research - March 30, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Resveratrol inhibits the development of obesity-related osteoarthritis via the TLR4 and PI3K/Akt signaling pathways.
CONCLUSIONS: Resveratrol may exert an anti-osteoarthritic effect by inhibiting TLR4 via the activation of PI3K/Akt signaling pathways. Resveratrol has potential as a drug for OA prevention. PMID: 30922122 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 30, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Magnesium lithospermate B inhibits titanium particles-induced osteoclast formation by c-fos and inhibiting NFATc1 expression.
In this study, we observed the effects of magnesium lithospermate B (MLB) on titanium particle-induced osteoclast activity in vitro. MATERIALS AND METHODS: RAW264.7 cells were treated with titanium particles (0.1 mg/mL) in the presence or absence of MLB (200 nmol/L). We evaluated the osteoclast formation, bone pits formation and tartrate-resistant acid phosphatase 5b (Tracp5b) levels. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to evaluate osteoclast differentiation-related genes (TRAF6, NFATc1, and c-fos) and protein expression. RESULTS: The number of osteoclasts, pit f...
Source: Connective Tissue Research - March 27, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

The involvement of the ERK-MAPK pathway in TGF- β1-mediated connexin43-gap junction formation in chondrocytes.
CONCLUSIONS: This study provides evidence to show the importance of the ERK-MAPK pathway in TGF-β1-mediated Cx43 expression and functional gap junction formation. PMID: 30897973 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 23, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Lithium chloride attenuates suppressed differentiation induced by mechanical strain in cementoblasts.
CONCLUSIONS: 2500-µ strain mechanical strain inhibited cementoblast differentiation activity in vitro, which could be alleviated by actviating Wnt/β-catenin signaling using lithium chloride. PMID: 30897979 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - March 23, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Silibinin's regulation of proliferation and collagen gene expressions of rat pancreatic β-cells cultured on types I and V collagen involves β-catenin nuclear translocation.
In this study, we report that the proliferation of INS-1 cells cultured on collagen I-coated dishes is enhanced, but it is inhibited on collagen V-coated dishes. Inhibitory proliferation on collagen V-coated is not due to apoptosis induction. Silibinin decreases hepatic glucose production and protects pancreatic β-cells, as a potential medicine for type II diabetes. Silibinin up-regulates the proliferation of cells cultured on both collagen I- and V-coated dishes. Collagen-coating regulates gene expression of collagen in a collagen type-related manner. Silibinin increases mRNA expression of collagen I in the cells on ...
Source: Connective Tissue Research - March 17, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis.
Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis. Connect Tissue Res. 2019 Feb 20;: Authors: Ma L, Liu Y, Zhao X, Li P, Jin Q Abstract To investigate whether systemic injection of rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis (OA). Ten-week-old male C57BL/6j wild-type (WT) mice and SOST-knockout (SOST-/-) mice were randomized to a sham group, a vehicle-treated group, and a rapamycin-treated group. Mice in the vehicle-treated group underwent destabilizing of the ...
Source: Connective Tissue Research - February 21, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

N-acetylcysteine Reduces Glutamate-Induced Cytotoxicity to Fibroblasts of Rat Supraspinatus Tendons.
Authors: Kim RJ, Hah YS, Gwark JY, Park HB Abstract PURPOSE: Neuronal theory regarding rotator cuff degeneration has developed from the findings that glutamate, an amino acid and an excitatory neurotransmitter, is present in increased concentrations in tendon tissues with tendinopathy and that glutamate induces cell death in fibroblasts of origin in rat supraspinatus tendon. The purpose of the current study was to determine whether N-acetylcysteine (NAC) has cytoprotective effects against glutamate-induced fibroblast death. MATERIALS AND METHODS: Primary cultured fibroblasts were obtained from rat supraspinatus...
Source: Connective Tissue Research - February 14, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

The effect of CPNE7 on periodontal regeneration.
CONCLUSION: These findings suggest that Cpne7 may have a functional role in periodontal regeneration by supporting periodontal cell attachment to cementum and facilitating physiological arrangement of PDL fibers. PMID: 30734591 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - February 10, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Diverticulosis and nine connective tissue disorders: epidemiological support for an association.
CONCLUSION: People with diverticulosis are more likely to have colonic extracellular matrix (ECM)/connective tissue alterations in anatomical areas other than the bowel, suggesting linked ECM/connective tissue pathology. Although biases may exist, the results indicate large-scale integrated studies are needed to investigate underlying genetic pathophysiology of colonic diverticula, together with fundamental biological studies to investigate cellular phenotypes and ECM changes. PMID: 30719942 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - February 7, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Fibrosis in distinct tissues.
Authors: Fertala A PMID: 30667273 [PubMed - in process] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 24, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Estimation of the elastic modulus of child cortical bone specimens via microindentation.
CONCLUSION: For the first time, NPCCB elastic modulus values are provided in longitudinal and transverse directions at the microscale level. PMID: 30646770 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 18, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Transforming Growth Factor- β1 Stimulates Mesenchymal Stem Cells Proliferation via Altering Cell Cycle through FAK-Akt-mTOR Pathway.
CONCLUSION: TGF-β1 triggered proliferation via accelerating G1/S cell cycle transition in BMSCs. The addition of TGF-β1 can activate Akt-mTOR-S6K1 pathway. Additionally, FAK was found to be involved in the process. Upon adding the FAK inhibitor, the activation of Akt-mTOR-S6K1 and TGF-β1-induced cell proliferation were both abrogated. Together, an insight understanding of how TGF-β1 influences BMSCs proliferation is achieved. This study provides a possible strategy of supplementing TGF-β1 in serum-free medium for in vitro expansion, which eventually would advance the production of clinical-grade MS...
Source: Connective Tissue Research - January 17, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

Interleukin-35 suppresses antitumor activity of circulating CD8+ T cells in osteosarcoma patients.
CONCLUSIONS: The current data indicated that IL-35 contributed to CD8+ T-cell dysfunction and limited antitumor immune response in osteosarcoma. PMID: 30616389 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - January 10, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

3D printing with silk: considerations and applications.
This article discusses recent research and printing parameters important for 3D printing with silk. PMID: 30558445 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - December 19, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs.
This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progenitor cells (ACPCs), and multipotent mesenchymal stromal cells (MSCs) embedded in these bio-inks. The incorporating of HAMA in gelMA/gellan bio-ink increased filament stability, as measured using a filament collapse assay, but did not influence (zone-specific) chondrogenesis of any of the cell types. Highest chondrogenic potential was observed for MSCs, followed by ACPCs, whi...
Source: Connective Tissue Research - December 14, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Proliferation of rheumatoid arthritis fibroblast-like synoviocytes is enhanced by IL-17-mediated autophagy through STAT3 activation.
Authors: Chang L, Feng X, Gao W Abstract Fibroblast-like synoviocytes (FLSs), with their tumor-like proliferation, play an important role in rheumatoid arthritis (RA), and interleukin-17 (IL-17) participates in RA pathology by affecting FLSs. The aims of this study were to investigate the effects of IL-17 on the proliferation and autophagy of FLSs and the role of signal transducer and activator of transcription-3 (STAT3) in RA. FLSs were treated with IL-17 at different concentrations (0, 1, 10, and 20 ng/mL); then, autophagy was assayed with western blotting, immunofluorescence and transmission electron microscopy....
Source: Connective Tissue Research - November 29, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Bay11-7082 facilitates wound healing by antagonizing mechanical injury- and TNF- α-induced expression of MMPs in posterior cruciate ligament.
CONCLUSIONS: SCs sever as the supporting cells that aggravate the TNF-α-induced MMPs accumulation in PCLfs. Inhibition of the expression of MMPs by Bay11-7082 is a promising way to facilitate the self-healing of PCL. PMID: 30372627 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 30, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.
Authors: Conoscenti G, Carfì Pavia F, Ongaro A, Brucato V, Goegele C, Schwarz S, Boccaccini AR, Stoelzel K, La Carrubba V, Schulze-Tanzil G Abstract Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. The aim of this work was to produ...
Source: Connective Tissue Research - October 25, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Fine-tuning the ubiquitin-proteasome system to treat pulmonary fibrosis.
Authors: Roque W, Summer R, Romero F Abstract Idiopathic pulmonary fibrosis (IPF) is an extremely aggressive lung disease that develops almost exclusively in older individuals, carries a very poor prognosis, and lacks any truly effective therapies. The current conceptual model is that IPF develops because of an age-related decline in the ability of the lung epithelium to regenerate after injury, largely due to death or senescence of epithelial progenitor cells in the distal airways. This loss of regenerative capacity is thought to initiate a chronic and ineffective wound-healing response, characterized by persisten...
Source: Connective Tissue Research - October 23, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Single vs. repeated matrix metalloproteinase-13 knockdown with intra-articular short interfering RNA administration in a murine osteoarthritis model.
Authors: Nakagawa R, Akagi R, Yamaguchi S, Enomoto T, Sato Y, Kimura S, Ogawa Y, Sadamasu A, Ohtori S, Sasho T Abstract PURPOSE: Our aims were 1) to estimate the duration of short interfering RNA (siRNA) effect on matrix metalloproteinase-13 (Mmp-13) levels by a single intra-articular injection using a mouse knee osteoarthritis (OA) model, and 2) to test whether repeated injections results in any additional suppressive effect on cartilage degradation compared to a single injection. MATERIALS AND METHODS: OA was induced in 9 weeks old male C57BL/6 mice by destabilization of medial meniscus (DMM). Chemically modi...
Source: Connective Tissue Research - October 23, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Multiscale molecular interactions of tendon.
Authors: Little D PMID: 30293484 [PubMed - in process] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 10, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Inhibition of Nf- ҝb Prevents Trauma-induced Heterotopic Ossification in Rat Model.
Inhibition of Nf-ҝb Prevents Trauma-induced Heterotopic Ossification in Rat Model. Connect Tissue Res. 2018 Oct 05;: Authors: Ju J, Yu D, Xue F, Zhao Y, Shi W, Pan M, Tang G, Xiao H Abstract Acquired heterotopic ossification (HO), the ectopic formation of bone in soft tissues, is a common cause of joint pain and confined motion. The pathogenesis of HO must be better understood to find a better prophylactic method. In the first part, we designed the brain-traumatic/burn/tenotomy rat model and testified its efficacy as HO model. 44 rats were randomly divided into experimental group and control group. Af...
Source: Connective Tissue Research - October 6, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Reactive oxygen species are responsible for the cell aggregation and production of pro-inflammatory mediators in phorbol ester (PMA)-treated U937 cells on gelatin-coated dishes through upregulation of autophagy.
CONCLUSION: The results suggest that gelatin-coating promotes the aggregation of PMA-treated U937 cells and the production of pro-inflammatory mediators by ROS-autophagy signaling pathway. PMID: 30277081 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - October 3, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Fibroblasts from recurrent fibrotic overgrowths reveal high rate of proliferation in vitro - findings from the study of hereditary and idiopathic gingival fibromatosis.
CONCLUSIONS: The study revealed that recurrent gingival overgrowths consist of highly fibrotic and dense connective tissue with numerous blood vessels and abundant fibroblasts. We also demonstrated that unlike fibroblasts derived from overgrowths, which did not present recurrence, fibroblasts derived from highly fibrotic and recurrent overgrowths maintain high rate of proliferation in vitro. PMID: 30231645 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 22, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Cellular, matrix, and mechano-biological differences in load-bearing versus positional tendons throughout development and aging: a narrative review.
CONCLUSIONS: The pheno-endotype of tendinopathy may be distinct between load-bearing tendons compared to positional tendons, which has translational implications with regard to preventing and managing tendinopathy. Better understanding of the molecular, cellular, and biomechanical pathophysiology underlying disease phenotypes, will allow more targeted/personalised treatment and therefore improve outcomes. PMID: 30231648 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 22, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Roadmap of molecular, compositional, and functional markers during embryonic tendon development.
Authors: Nguyen PK, Pan XS, Li J, Kuo CK Abstract Tendon is a specialized connective tissue that connects muscle to bone, thereby enabling musculoskeletal movement. Tendon injury leads to formation of tissue with aberrant functional properties. Current approaches to treat tendon injuries, including surgical repair and tissue engineering, have not achieved normal tendon. A roadmap of markers could help with identifying when mis-steps occur during aberrant tendon formation and providing instructions for normal tendon formation. We propose this roadmap should be based on the embryo-the perfect model of tissue formatio...
Source: Connective Tissue Research - September 22, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Peripheral nerve injury, scarring, and recovery.
Authors: Wang ML, Rivlin M, Graham JG, Beredjiklian PK Abstract Peripheral nerve injuries (PNI) resulting from trauma can be severe and permanently debilitating. Despite the armamentarium of meticulous microsurgical repair techniques that includes direct repair, grafting of defects with autograft nerve, and grafting with cadaveric allografts, approximately one-third of all PNI demonstrate incomplete recovery with poor restoration of function. This may include total loss or incomplete recovery of motor and/or sensory function, chronic pain, muscle atrophy, and profound weakness, which can result in lifelong morbidit...
Source: Connective Tissue Research - September 8, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Arthrofibrosis and Large Joint Scarring.
Authors: Chen AF, Lee YS, Seidl AJ, Abboud JA PMID: 30173570 [PubMed - as supplied by publisher] (Source: Connective Tissue Research)
Source: Connective Tissue Research - September 5, 2018 Category: Research Tags: Connect Tissue Res Source Type: research