An evaluation of a multiplex PCR assay for the detection of Treponema pallidum, HSV-1, and HSV-2
Diagn Microbiol Infect Dis. 2023 Apr 19;106(3):115958. doi: 10.1016/j.diagmicrobio.2023.115958. Online ahead of print.ABSTRACTMultiplex PCR can utilize limited clinical material and is more cost-effective and expected to be used for the detection of Treponema pallidum, herpes simplex virus type 1 and 2 (HSV-1,2). We established a multiplex TP-HSV1-HSV2 Polymerase Chain Reaction (multiplex PCR) targeting the conserved regions of the PolA gene of TP and the UL42 gene of HSV1 and HSV2 to test skin lesions of 115 patients suspected of having TP and HSV1/2 infections. The laboratory sensitivities for all 3 pathogens were 300 co...
Source: Herpes - May 12, 2023 Category: Infectious Diseases Authors: Liufeng Yuan Deju Xia Qian Zhou Wenqi Xu Sihong Xu Yueping Yin Source Type: research
Identification of oxysterol synthetic analogs as a novel class of late-stage inhibitors of herpes simplex virus 2 replication
In this study, we screened selected members of an in-house synthesized library of oxysterol analogues for their activity against HSV-2, identifying three compounds, named PFM064, PFM067, and PFM069, endowed with 50% effective concentrations (EC50) in the micromolar range, without exerting any apparent cytotoxicity. Moreover, the results obtained showed the ability of the novel derivatives to inhibit both cell-to-cell fusion induced by HSV-2, and the production of an intracellular viral progeny. Further experiments performed with PFM067 (which was selected for more-in-depth studies as the most effective synthetic analog) sh...
Source: Antiviral Research - May 10, 2023 Category: Virology Authors: Andrea Civra Matteo Costantino Giulia Ronchi Lorenzo Pontini Giuseppe Poli Maura Marinozzi David Lembo Source Type: research
Identification of oxysterol synthetic analogs as a novel class of late-stage inhibitors of herpes simplex virus 2 replication
In this study, we screened selected members of an in-house synthesized library of oxysterol analogues for their activity against HSV-2, identifying three compounds, named PFM064, PFM067, and PFM069, endowed with 50% effective concentrations (EC50) in the micromolar range, without exerting any apparent cytotoxicity. Moreover, the results obtained showed the ability of the novel derivatives to inhibit both cell-to-cell fusion induced by HSV-2, and the production of an intracellular viral progeny. Further experiments performed with PFM067 (which was selected for more-in-depth studies as the most effective synthetic analog) sh...
Source: Cell Research - May 10, 2023 Category: Cytology Authors: Andrea Civra Matteo Costantino Giulia Ronchi Lorenzo Pontini Giuseppe Poli Maura Marinozzi David Lembo Source Type: research
Identification of oxysterol synthetic analogs as a novel class of late-stage inhibitors of herpes simplex virus 2 replication
In this study, we screened selected members of an in-house synthesized library of oxysterol analogs for their activity against HSV-2, identifying three compounds, named PFM064, PFM067, and PFM069, endowed with 50% effective concentrations (EC50) in the micromolar range, without exerting any apparent cytotoxicity. Moreover, the results obtained showed the ability of the novel derivatives to inhibit both cell-to-cell fusion induced by HSV-2, and the production of an intracellular viral progeny. Further experiments performed with PFM067 (which was selected for more-in-depth studies as the most effective synthetic analog) show...
Source: Herpes - May 10, 2023 Category: Infectious Diseases Authors: Andrea Civra Matteo Costantino Giulia Ronchi Lorenzo Pontini Giuseppe Poli Maura Marinozzi David Lembo Source Type: research
Identification of oxysterol synthetic analogs as a novel class of late-stage inhibitors of herpes simplex virus 2 replication
In this study, we screened selected members of an in-house synthesized library of oxysterol analogs for their activity against HSV-2, identifying three compounds, named PFM064, PFM067, and PFM069, endowed with 50% effective concentrations (EC50) in the micromolar range, without exerting any apparent cytotoxicity. Moreover, the results obtained showed the ability of the novel derivatives to inhibit both cell-to-cell fusion induced by HSV-2, and the production of an intracellular viral progeny. Further experiments performed with PFM067 (which was selected for more-in-depth studies as the most effective synthetic analog) show...
Source: Antiviral Research - May 10, 2023 Category: Virology Authors: Andrea Civra Matteo Costantino Giulia Ronchi Lorenzo Pontini Giuseppe Poli Maura Marinozzi David Lembo Source Type: research
Viruses, Vol. 15, Pages 1148: Novel Adjuvant S-540956 Targets Lymph Nodes and Reduces Genital Recurrences and Vaginal Shedding of HSV-2 DNA When Administered with HSV-2 Glycoprotein D as a Therapeutic Vaccine in Guinea Pigs
Gary H. Cohen
Kazufumi Katayama
Harvey M. Friedman
Herpes simplex virus type 2 (HSV-2) is a leading cause of genital ulcer disease and a major risk factor for acquisition and transmission of HIV. Frequent recurrent genital lesions and concerns about transmitting infection to intimate partners affect the quality of life of infected individuals. Therapeutic vaccines are urgently needed to reduce the frequency of genital lesions and transmission. S-540956 is a novel vaccine adjuvant that contains CpG oligonucleotide ODN2006 annealed to its complementary sequence and conjugated to a lipid that targets the adjuvant to...
Source: Viruses - May 10, 2023 Category: Virology Authors: Sita Awasthi Motoyasu Onishi John M. Lubinski Bernard T. Fowler Alexis M. Naughton Lauren M. Hook Kevin P. Egan Masaki Hagiwara Seiki Shirai Akiho Sakai Takayuki Nakagawa Kumiko Goto Osamu Yoshida Alisa J. Stephens Grace Choi Gary H. Cohen Kazufumi Kataya Tags: Article Source Type: research
Polyphenylene carboxymethylene (PPCM), the active component of the topical contraceptive Yaso-GEL, exhibits potent antimicrobial activity against < em > Neisseria gonorrhoeae < /em > in preclinical studies
CONCLUSIONS: Yaso-GEL containing the API PPCM showed significant activity against N. gonorrhoeae in vitro and in vivo in a female mouse model. These data support further development of Yaso-GEL as an inexpensive, non-hormonal and non-systemic product with both contraceptive and antimicrobial activity against gonorrhea and other common sexually transmitted infections (STIs). Such multipurpose prevention technology products are needed by women in all economic, social and cultural circumstances to prevent unintended pregnancy and STIs.PMID:37156608 | DOI:10.1136/sextrans-2022-055596 (Source: Herpes)
Source: Herpes - May 8, 2023 Category: Infectious Diseases Authors: Michelle Pilligua-Lucas Rok Tkavc Sarah K Bash Barbara B North Mary B Weitzel Ann E Jerse Source Type: research
Epidemiological Survey of Human Alphaherpesvirus 2 (HSV-2) Infection in Indigenous People of Dourados Municipality, Central Brazil
Trop Med Infect Dis. 2023 Mar 29;8(4):197. doi: 10.3390/tropicalmed8040197.ABSTRACTSexually transmitted Human alphaherpesvirus 2 (HSV-2) causes genital ulcers, especially among sexually active adolescents and adults. We estimated the exact prevalence of anti-HSV-2 antibodies and correlated it with the demographic and behavioral aspects of the Indigenous population of the Jaguapirú and Bororó villages (Dourados, Mato Grosso do Sul (MS), Brazil). In total, 1360 individuals (>18 years old) were administered serologic tests. The prevalence of anti-HSV-2 IgM was 12.9%, that of anti-HSV-2 IgG was 57.2%, and 8.5% cases teste...
Source: Herpes - April 27, 2023 Category: Infectious Diseases Authors: Fl ávia Freitas de Oliveira Bonfim Livia Melo Villar Julio Croda Solange Rodrigues da Silva Crhistinne Cavalheiro Maymone Gon çalves Vivianne de Oliveira Landgraf de Castro Grazielli Rocha de Rezende Romeira Gabriela Alves Cesar Sabrina Moreira Dos Sant Source Type: research
Genital Herpes Infection: Progress and Problems
Infect Dis Clin North Am. 2023 Jun;37(2):351-367. doi: 10.1016/j.idc.2023.02.011.ABSTRACTGenital herpes (GH) is a sexually transmitted infection causing recurrent, self-limited genital, buttock, and thigh ulcerations. Symptoms range from unrecognized or mild to severe with frequent recurrences. Herpes simplex viruses (HSV) type-1 or type-2 cause GH. HSV establishes latency in sacral ganglia and causes lifelong infection. Viral reactivation leads to genital ulceration or asymptomatic shedding which may lead to transmission. HSV infection during pregnancy can cause fulminant hepatitis and neonatal transmission. Severe and at...
Source: Herpes - April 27, 2023 Category: Infectious Diseases Authors: Nicholas Van Wagoner Fuad Qushair Christine Johnston Source Type: research
Epidemiological Survey of Human Alphaherpesvirus 2 (HSV-2) Infection in Indigenous People of Dourados Municipality, Central Brazil
Trop Med Infect Dis. 2023 Mar 29;8(4):197. doi: 10.3390/tropicalmed8040197.ABSTRACTSexually transmitted Human alphaherpesvirus 2 (HSV-2) causes genital ulcers, especially among sexually active adolescents and adults. We estimated the exact prevalence of anti-HSV-2 antibodies and correlated it with the demographic and behavioral aspects of the Indigenous population of the Jaguapirú and Bororó villages (Dourados, Mato Grosso do Sul (MS), Brazil). In total, 1360 individuals (>18 years old) were administered serologic tests. The prevalence of anti-HSV-2 IgM was 12.9%, that of anti-HSV-2 IgG was 57.2%, and 8.5% cases teste...
Source: Herpes - April 27, 2023 Category: Infectious Diseases Authors: Fl ávia Freitas de Oliveira Bonfim Livia Melo Villar Julio Croda Solange Rodrigues da Silva Crhistinne Cavalheiro Maymone Gon çalves Vivianne de Oliveira Landgraf de Castro Grazielli Rocha de Rezende Romeira Gabriela Alves Cesar Sabrina Moreira Dos Sant Source Type: research
Genital Herpes Infection
Genital herpes (GH) is a sexually transmitted infection causing recurrent, self-limited genital, buttock, and thigh ulcerations. Symptoms range from unrecognized or mild to severe with frequent recurrences. Herpes simplex viruses (HSV) type-1 or type-2 cause GH. HSV establishes latency in sacral ganglia and causes lifelong infection. Viral reactivation leads to genital ulceration or asymptomatic shedding which may lead to transmission. HSV infection during pregnancy can cause fulminant hepatitis and neonatal transmission. Severe and atypical manifestations are seen in immunocompromised people. Guanosine analogs treat sympt...
Source: Infectious Diseases Clinics of North America - April 26, 2023 Category: Infectious Diseases Authors: Nicholas Van Wagoner, Fuad Qushair, Christine Johnston Source Type: research
Postpartum Neonatal Disseminated Herpes Simplex Virus-1 Infection in Which Herpes Simplex Virus-1 Was Detected in Mother's Breast Milk
AbstractA 17-d-old girl was diagnosed with disseminated herpes simplex virus-1 infection-associated hemophagocytic lymphohistiocytosis. The virus was detected in the neonate ’s blood and mandible. The neonate was treated with dexamethasone and acyclovir and discharged without neurological sequelae. The mother had no history of a herpes simplex virus-1 infection and did not have visible genital herpetic lesions; moreover, the neonate was delivered via an elective cesar ean section. However, the day before the delivery, the mother had met with the neonate’s grandmother, who had herpes labialis. Viral DNA was detected in ...
Source: Indian Journal of Pediatrics - April 21, 2023 Category: Pediatrics Source Type: research
Cutaneous infections from viral sources in solid organ transplant recipients
CONCLUSIONS: The occurrence of cutaneous viral infections in SOTRs is directly associated with the duration and intensity of immunosuppressive therapy. Patients at higher risk were those taking drugs with a stronger impact on cellular immunity and/or those on an immunosuppressive regimen comprising various drugs.PMID:37085124 | DOI:10.1016/j.trim.2023.101838 (Source: Transplant Immunology)
Source: Transplant Immunology - April 21, 2023 Category: Transplant Surgery Authors: Isadora Zago Miotto Cyro Festa Neto Walmar Roncalli Pereira de Oliveira Source Type: research
High seroprevalence of syphilis and genital herpes in migrants in transit in Chiapas, MexicoAlta soropreval ência de sífilis e herpes genital em migrantes em trânsito por Chiapas, México
CONCLUSIONS: A high prevalence of syphilis and HSV-2 was found among the migrant population evaluated. Syphilis is confirmed as a re-emerging infection, even in women. Migrants have vulnerabilities associated with sexual behavior, so prevention, diagnosis, and treatment measures should be focused on this population group.PMID:37082541 | PMC:PMC10100594 | DOI:10.26633/RPSP.2023.71 (Source: Pan American Journal of Public Health)
Source: Pan American Journal of Public Health - April 21, 2023 Category: International Medicine & Public Health Authors: Miguel Angel S ánchez-Alemán Angel Emanuel Rogel-Gonz ález Santa Garc ía-Cisneros Mar ía Olamendi-Portugal Dayana Nict é Vergara-Ortega H éctor Armando Rincón-León Antonia Herrera-Ortiz Source Type: research
Cutaneous infections from viral sources in solid organ transplant recipients
CONCLUSIONS: The occurrence of cutaneous viral infections in SOTRs is directly associated with the duration and intensity of immunosuppressive therapy. Patients at higher risk were those taking drugs with a stronger impact on cellular immunity and/or those on an immunosuppressive regimen comprising various drugs.PMID:37085124 | DOI:10.1016/j.trim.2023.101838 (Source: Transplant Immunology)
Source: Transplant Immunology - April 21, 2023 Category: Transplant Surgery Authors: Isadora Zago Miotto Cyro Festa Neto Walmar Roncalli Pereira de Oliveira Source Type: research
