Successful Confirmation of Dual Genital Herpes Co-Infection with Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Using Unbiased Metagenomic Next-Generation Sequencing
Viruses. 2023 Sep 20;15(9):1957. doi: 10.3390/v15091957.ABSTRACTDual co-infection with both HSV-1 and HSV-2 is rare, with few cases reported in the literature. In this case report, we describe the successful use of unbiased metagenomic next-generation sequencing (mNGS) as a rapid and alternative method for confirming dual genital herpes co-infection. Our case involves a 74-year-old woman who presented with genital lesions and initially tested positive for both HSV-1 and HSV-2 via the Luminex ARIES HSV 1&2 assay. The entire mNGS process, from nucleic acid extraction to result analysis, was completed in less than 48 h. U...
Source: Herpes - September 28, 2023 Category: Infectious Diseases Authors: Chun Kiat Lee Sau Yoke Ng Chean Nee Chai Yu Feng Lim Tiffany Jingyan Hu Ogestelli Fabia Lee Gabriel Yan Source Type: research
Mechanistic of Vesicular Ethosomes and Elastic Liposomes on Permeation Profiles of Acyclovir across Artificial Membrane, Human Cultured EpiDerm, and Rat Skin: In Vitro-Ex Vivo Study
Pharmaceutics. 2023 Aug 24;15(9):2189. doi: 10.3390/pharmaceutics15092189.ABSTRACTAcyclovir (ACV) controls cutaneous herpes, genital herpes, herpes keratitis, varicella zoster, and chickenpox. From previously reported ACV formulations, we continued to explore the permeation behavior of the optimized ACV loaded optimized ethosome (ETHO2R) and elastic liposome (ELP3R) and their respective carbopol gels across artificial membrane, cultured human EpiDerm, and rat skin. Transepidermal water loss (TEWL), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and atomic force microscopy (AFM) were used to ...
Source: Herpes - September 28, 2023 Category: Infectious Diseases Authors: Afzal Hussain Mohammad A Altamimi Obaid Afzal Abdulmalik S A Altamimi Mohhammad Ramzan Tahir Khuroo Source Type: research
Successful Confirmation of Dual Genital Herpes Co-Infection with Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Using Unbiased Metagenomic Next-Generation Sequencing
Viruses. 2023 Sep 20;15(9):1957. doi: 10.3390/v15091957.ABSTRACTDual co-infection with both HSV-1 and HSV-2 is rare, with few cases reported in the literature. In this case report, we describe the successful use of unbiased metagenomic next-generation sequencing (mNGS) as a rapid and alternative method for confirming dual genital herpes co-infection. Our case involves a 74-year-old woman who presented with genital lesions and initially tested positive for both HSV-1 and HSV-2 via the Luminex ARIES HSV 1&2 assay. The entire mNGS process, from nucleic acid extraction to result analysis, was completed in less than 48 h. U...
Source: Herpes - September 28, 2023 Category: Infectious Diseases Authors: Chun Kiat Lee Sau Yoke Ng Chean Nee Chai Yu Feng Lim Tiffany Jingyan Hu Ogestelli Fabia Lee Gabriel Yan Source Type: research
Mechanistic of Vesicular Ethosomes and Elastic Liposomes on Permeation Profiles of Acyclovir across Artificial Membrane, Human Cultured EpiDerm, and Rat Skin: In Vitro-Ex Vivo Study
Pharmaceutics. 2023 Aug 24;15(9):2189. doi: 10.3390/pharmaceutics15092189.ABSTRACTAcyclovir (ACV) controls cutaneous herpes, genital herpes, herpes keratitis, varicella zoster, and chickenpox. From previously reported ACV formulations, we continued to explore the permeation behavior of the optimized ACV loaded optimized ethosome (ETHO2R) and elastic liposome (ELP3R) and their respective carbopol gels across artificial membrane, cultured human EpiDerm, and rat skin. Transepidermal water loss (TEWL), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and atomic force microscopy (AFM) were used to ...
Source: Herpes - September 28, 2023 Category: Infectious Diseases Authors: Afzal Hussain Mohammad A Altamimi Obaid Afzal Abdulmalik S A Altamimi Mohhammad Ramzan Tahir Khuroo Source Type: research
Cervical ulcer caused by group B streptococcus with bacterial vaginosis: a case report
BMC Womens Health. 2023 Sep 27;23(1):516. doi: 10.1186/s12905-023-02665-w.ABSTRACTThe female genital ulcer is a manifestation of many diseases, which may vary depending on the etiology, disease duration, age, and host immunity. A middle-aged (40-50 years) woman had a 4-month history of vaginal bleeding. The results of syphilis, herpes, the cervical cancer, tuberculosis, and fungi or acute cervical inflammation caused by Chlamydia trachomatis and Mycoplasma hominis were negative through the blood test and the biopsy. Cervical discharge culture revealed positive for group B Streptococcus and bacterial vaginosis. The patient ...
Source: Herpes - September 27, 2023 Category: Infectious Diseases Authors: Yi Chen Dan Wu Source Type: research
Incidence of herpes simplex virus type 2 positivity among women living with human immunodeficiency virus in South Africa
CONCLUSION: After acute HIV infection in women, the incidence of HSV-2 PCR positivity was associated with younger age, BV diagnosis and lower CD4 count.PMID:37751624 | DOI:10.1177/09564624231201189 (Source: Herpes)
Source: Herpes - September 26, 2023 Category: Infectious Diseases Authors: Kwabena Asare Sinaye Ngcapu Farzana Osman Adrian Mindel Nivashnee Naicker Mlungisi Khanyile Salim S Abdool Karim Andrew Tomita Nigel Garrett Source Type: research
Viruses, Vol. 15, Pages 1957: Successful Confirmation of Dual Genital Herpes Co-Infection with Herpes Simplex Virus 1 and Herpes Simplex Virus 2 Using Unbiased Metagenomic Next-Generation Sequencing
Gabriel Yan
Dual co-infection with both HSV-1 and HSV-2 is rare, with few cases reported in the literature. In this case report, we describe the successful use of unbiased metagenomic next-generation sequencing (mNGS) as a rapid and alternative method for confirming dual genital herpes co-infection. Our case involves a 74-year-old woman who presented with genital lesions and initially tested positive for both HSV-1 and HSV-2 via the Luminex ARIES HSV 1&2 assay. The entire mNGS process, from nucleic acid extraction to result analysis, was completed in less than 48 h. Using mNGS, we identified mapped reads ...
Source: Viruses - September 20, 2023 Category: Virology Authors: Chun Kiat Lee Sau Yoke Ng Chean Nee Chai Yu Feng Lim Tiffany Jingyan Hu Ogestelli Fabia Lee Gabriel Yan Tags: Brief Report Source Type: research
HIV/STD prevalence and test uptake among african in Guangzhou, China: an analysis of data from hospital-based surveillance
BMC Infect Dis. 2023 Sep 13;23(1):597. doi: 10.1186/s12879-023-08590-5.ABSTRACTHuman immunodeficiency virus (HIV) and sexually transmitted diseases (STDs) cause substantial morbidity and mortality both in African and China. However, there is limited data available on the prevalence of HIV/STDs and the uptake of testing experience ever during in China among African migrants. A venue-based survey was conducted at a tertiary hospital in Guangzhou to investigate the prevalence of HIV/STDs through laboratory testing and identify the associated factors. A total of 200 eligible participants completed the survey and bring into the...
Source: Herpes - September 13, 2023 Category: Infectious Diseases Authors: Mingzhou Xiong Menglan Yang Peizhen Zhao Shujie Huang Cheng Wang Source Type: research
Differential diagnosis of stage 2, 3 and 4 pressure injuries of the pelvis and lower extremity: a case series
J Wound Care. 2023 Sep 1;32(Sup9):S22-S36. doi: 10.12968/jowc.2023.32.Sup9.S22.ABSTRACTConstant, unrelieved pressure of local tissue, particularly over bony prominences, may provoke damage that progresses to necrosis and pressure injury (PI). Differentiating PIs from conditions of similar appearance is imperative to minimising complications and implementing prompt treatment. This case series describes several conditions that may be mistaken for a PI. Outlined are the key differences in patient history, presentation and clinical cues that assist in correctly identifying the true pathology behind these conditions. Conditions...
Source: Herpes - September 8, 2023 Category: Infectious Diseases Authors: Jennifer Caudill Tim Chrisman Joanna Chen Kelsee Zajac Richard Simman Source Type: research
Differential diagnosis of stage 2, 3 and 4 pressure injuries of the pelvis and lower extremity: a case series
J Wound Care. 2023 Sep 1;32(Sup9):S22-S36. doi: 10.12968/jowc.2023.32.Sup9.S22.ABSTRACTConstant, unrelieved pressure of local tissue, particularly over bony prominences, may provoke damage that progresses to necrosis and pressure injury (PI). Differentiating PIs from conditions of similar appearance is imperative to minimising complications and implementing prompt treatment. This case series describes several conditions that may be mistaken for a PI. Outlined are the key differences in patient history, presentation and clinical cues that assist in correctly identifying the true pathology behind these conditions. Conditions...
Source: Herpes - September 8, 2023 Category: Infectious Diseases Authors: Jennifer Caudill Tim Chrisman Joanna Chen Kelsee Zajac Richard Simman Source Type: research
Increased Risk of Infertility in Women Infected with Human Papillomavirus
CONCLUSION: Since HPV infection is asymptomatic, periodic screening of women in reproductive age especially infertile couples is recommended for early diagnosis and prevention of infection progression and cross contamination.PMID:37663425 | PMC:PMC10471950 | DOI:10.18502/jri.v24i3.13275 (Source: Herpes)
Source: Herpes - September 4, 2023 Category: Infectious Diseases Authors: Parastou Heidari Pebdeni Fereshteh Saffari Hamid Reza Mollaei Toraj Reza Mirshekari Robabeh Hosseini Sadat Victoria Habibzadeh Lida Saeed Moslem Taheri Soodejani Roya Ahmadrajabi Source Type: research
Increased Risk of Infertility in Women Infected with Human Papillomavirus
CONCLUSION: Since HPV infection is asymptomatic, periodic screening of women in reproductive age especially infertile couples is recommended for early diagnosis and prevention of infection progression and cross contamination.PMID:37663425 | PMC:PMC10471950 | DOI:10.18502/jri.v24i3.13275 (Source: Herpes)
Source: Herpes - September 4, 2023 Category: Infectious Diseases Authors: Parastou Heidari Pebdeni Fereshteh Saffari Hamid Reza Mollaei Toraj Reza Mirshekari Robabeh Hosseini Sadat Victoria Habibzadeh Lida Saeed Moslem Taheri Soodejani Roya Ahmadrajabi Source Type: research
Boosting of vaginal HSV-2-specific B and T cell responses by intravaginal therapeutic immunization results in diminished recurrent HSV-2 disease
In this study, a therapeutic vaccine given intravaginally resulted in significantly better protection against HSV-2 disease than immunization with the same vaccine by a conventional route. Immunization by the intravaginal route resulted in greater stimulation of vaginal-resident, virus-specific cells that produced antibody and produced immune molecules to rapidly clear virus.PMID:37655939 | DOI:10.1128/jvi.00669-23 (Source: Herpes)
Source: Herpes - September 1, 2023 Category: Infectious Diseases Authors: Nigel Bourne Celeste A Keith Aaron L Miller Richard B Pyles Gary Cohen Gregg N Milligan Source Type: research
Interferon inhibits the release of herpes simplex virus-1 from the axons of sensory neurons
In this study, we determined the effects of type I, II, and III IFNs on HSV-1 release from sensory axons. Using compartmentalized microfluidic devices separating axons from the neuronal cell bodies, we showed that treating axons with type I (IFNα and IFNβ), type II (IFNγ), and type III (IFNλ) IFNs inhibited the release of HSV-1 from axons, without altering the anterograde axonal transport of viral components. Furthermore, we showed that only type II IFN induced a cell-wide response following axonal treatment, with phosphorylated STAT1 and STAT3 present along axons and in the neuronal cell body, whereas type I and III I...
Source: Herpes - September 1, 2023 Category: Infectious Diseases Authors: Kevin Danastas Gerry Guo Jessica Merjane Nathan Hong Ava Larsen Monica Miranda-Saksena Anthony L Cunningham Source Type: research
Boosting of vaginal HSV-2-specific B and T cell responses by intravaginal therapeutic immunization results in diminished recurrent HSV-2 disease
In this study, a therapeutic vaccine given intravaginally resulted in significantly better protection against HSV-2 disease than immunization with the same vaccine by a conventional route. Immunization by the intravaginal route resulted in greater stimulation of vaginal-resident, virus-specific cells that produced antibody and produced immune molecules to rapidly clear virus.PMID:37655939 | DOI:10.1128/jvi.00669-23 (Source: Herpes)
Source: Herpes - September 1, 2023 Category: Infectious Diseases Authors: Nigel Bourne Celeste A Keith Aaron L Miller Richard B Pyles Gary Cohen Gregg N Milligan Source Type: research
