Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1
This study aimed to evaluate the impact of Aluminum Oxide Nanoparticles (Al2O3-NPs) on HSV-1 infection. Characterization of Al2O3-NPs was performed using field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and high-resolution transmission electron microscopy (HRTEM). The MTT test was used to investigate the toxicity action of Al2O3-NPs on viable cells. Quantitative Real-Time PCR (qRT-PCR)and TCID50 assays were used to achieve the antiherpetic performance Al2O3-NPs.Indirect immunofluorescence assay (IFA) was performed to determine the inhibitory impact of Al2O3-NPs o...
Source: Herpes - June 14, 2023 Category: Infectious Diseases Authors: A K Almansorri H M H Al-Shirifi S Al-Musawi B B Ahmed Source Type: research
High Frequencies of Antiviral Effector Memory T < sub > EM < /sub > Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
bioRxiv. 2023 May 24:2023.05.23.542021. doi: 10.1101/2023.05.23.542021. Preprint.ABSTRACTVaginal mucosa-resident anti-viral effector memory B- and T cells appeared to play a crucial role in protection against genital herpes. However, how to mobilize such protective immune cells into the vaginal tissue close to infected epithelial cells remains to be determined. In the present study, we investigate whether and how, CCL28, a major mucosal-associated chemokine, mobilizes effector memory B- and T cells in leading to protecting mucosal surfaces from herpes infection and disease. The CCL28 is a chemoattractant for the CCR10 rece...
Source: Herpes - June 9, 2023 Category: Infectious Diseases Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Mahmoud Singer Nakayama Takashi Hawa Vahed Lbachir BenMohamed Source Type: research
High Frequencies of Antiviral Effector Memory T < sub > EM < /sub > Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
bioRxiv. 2023 May 24:2023.05.23.542021. doi: 10.1101/2023.05.23.542021. Preprint.ABSTRACTVaginal mucosa-resident anti-viral effector memory B- and T cells appeared to play a crucial role in protection against genital herpes. However, how to mobilize such protective immune cells into the vaginal tissue close to infected epithelial cells remains to be determined. In the present study, we investigate whether and how, CCL28, a major mucosal-associated chemokine, mobilizes effector memory B- and T cells in leading to protecting mucosal surfaces from herpes infection and disease. The CCL28 is a chemoattractant for the CCR10 rece...
Source: Herpes - June 9, 2023 Category: Infectious Diseases Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Mahmoud Singer Nakayama Takashi Hawa Vahed Lbachir BenMohamed Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Herpes - June 8, 2023 Category: Infectious Diseases Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Protocol for analyzing γδ T cells from the vagina of diet-induced obese mice using single-cell RNA sequencing and flow cytometry
We describe steps for isolating single cells from vaginal tissue and analyzing cells using single-cell RNA sequencing and flow cytometry. We then detail confirmation of the γδ T cell phenotype in vitro. For complete details on the use and execution of this protocol, please refer to Park et al.1.PMID:37289592 | DOI:10.1016/j.xpro.2023.102278 (Source: Herpes)
Source: Herpes - June 8, 2023 Category: Infectious Diseases Authors: In Kang Jang Hyun Park Yumin Kim Myoung Seung Kwon Heung Kyu Lee Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Comparison of Effects of Multiple Adjuvants and Immunization Routes on the Immunogenicity and Protection of HSV-2 gD Subunit Vaccine
Immunol Lett. 2023 Jun 6:S0165-2478(23)00097-4. doi: 10.1016/j.imlet.2023.06.004. Online ahead of print.ABSTRACTGenital herpes caused by herpes simplex virus type 2 (HSV-2) poses a global health issue. HSV-2 infection increases the risk of acquiring HIV infection. Studies have demonstrated that HSV-2 subunit vaccines have potential benefits, but require adjuvants to induce a balanced Th1/Th2 response. To develop a novel, effective vaccine, in this study, a truncated glycoprotein D (aa 1-285) of HSV-2 was formulated with an Al(OH)3 adjuvant, three squalene adjuvants, MF59, AS03, and AS02, or a mucosal adjuvant, bacterium-li...
Source: Immunology Letters - June 8, 2023 Category: Allergy & Immunology Authors: Mingming Wan Xiao Yang Zhijun Chen Weiheng Su Linjun Cai Ali Hou Bo Sun Yong Zhang Wei Kong Chunlai Jiang Yan Zhou Source Type: research
Clinico-epidemiological profile of genital dermatoses in people living with HIV: A shifting paradigm from venereal to nonvenereal dermatoses
Conclusion: This study showed that nonvenereal genital dermatoses are more common than STIs in people living with HIV. Our findings reiterate the fact that genital lesions should be approached with caution as a presumptive and hasty diagnosis of STI adds greatly to the morbidity of the patient in terms of guilt and shame, and adversely affects the quality of life. (Source: Indian Journal of Sexually Transmitted Diseases)
Source: Indian Journal of Sexually Transmitted Diseases - June 6, 2023 Category: Infectious Diseases Authors: Supreet Kaur Dhillon Mahendra M Kura Source Type: research
Novel Adjuvant S-540956 Targets Lymph Nodes and Reduces Genital Recurrences and Vaginal Shedding of HSV-2 DNA When Administered with HSV-2 Glycoprotein D as a Therapeutic Vaccine in Guinea Pigs
Viruses. 2023 May 10;15(5):1148. doi: 10.3390/v15051148.ABSTRACTHerpes simplex virus type 2 (HSV-2) is a leading cause of genital ulcer disease and a major risk factor for acquisition and transmission of HIV. Frequent recurrent genital lesions and concerns about transmitting infection to intimate partners affect the quality of life of infected individuals. Therapeutic vaccines are urgently needed to reduce the frequency of genital lesions and transmission. S-540956 is a novel vaccine adjuvant that contains CpG oligonucleotide ODN2006 annealed to its complementary sequence and conjugated to a lipid that targets the adjuvant...
Source: Herpes - May 27, 2023 Category: Infectious Diseases Authors: Sita Awasthi Motoyasu Onishi John M Lubinski Bernard T Fowler Alexis M Naughton Lauren M Hook Kevin P Egan Masaki Hagiwara Seiki Shirai Akiho Sakai Takayuki Nakagawa Kumiko Goto Osamu Yoshida Alisa J Stephens Grace Choi Gary H Cohen Kazufumi Katayama Harv Source Type: research
Novel Adjuvant S-540956 Targets Lymph Nodes and Reduces Genital Recurrences and Vaginal Shedding of HSV-2 DNA When Administered with HSV-2 Glycoprotein D as a Therapeutic Vaccine in Guinea Pigs
Viruses. 2023 May 10;15(5):1148. doi: 10.3390/v15051148.ABSTRACTHerpes simplex virus type 2 (HSV-2) is a leading cause of genital ulcer disease and a major risk factor for acquisition and transmission of HIV. Frequent recurrent genital lesions and concerns about transmitting infection to intimate partners affect the quality of life of infected individuals. Therapeutic vaccines are urgently needed to reduce the frequency of genital lesions and transmission. S-540956 is a novel vaccine adjuvant that contains CpG oligonucleotide ODN2006 annealed to its complementary sequence and conjugated to a lipid that targets the adjuvant...
Source: Herpes - May 27, 2023 Category: Infectious Diseases Authors: Sita Awasthi Motoyasu Onishi John M Lubinski Bernard T Fowler Alexis M Naughton Lauren M Hook Kevin P Egan Masaki Hagiwara Seiki Shirai Akiho Sakai Takayuki Nakagawa Kumiko Goto Osamu Yoshida Alisa J Stephens Grace Choi Gary H Cohen Kazufumi Katayama Harv Source Type: research
Mucosal CCL28 Chemokine Improves Protection against Genital Herpes through Mobilization of Antiviral Effector Memory CCR10+CD44+ CD62L-CD8+ T Cells and Memory CCR10+B220+CD27+ B Cells into the Infected Vaginal Mucosa
In this study, we investigated the role of the CCL28/CCR10 chemokine axis in mobilizing protective antiviral B and T cell subsets into the VM site of herpes infection. We report a significant increase in the frequencies of HSV-specific memory CCR10+CD44+CD8+ T cells, expressing high levels of CCR10, in herpes-infected asymptomatic (ASYMP) women compared with symptomatic women. Similarly, a significant increase in the CCL28 chemokine (a ligand of CCR10), was detected in the VM of herpes-infected ASYMP C57BL/6 mice, associated with the mobilization of high frequencies of HSV-specific effector memory CCR10+CD44+CD62L-CD8+ TEM...
Source: Journal of Immunology - May 24, 2023 Category: Allergy & Immunology Authors: Nisha Rajeswari Dhanushkodi Swayam Prakash Afshana Quadiri Latifa Zayou Ruchi Srivastava Jennifer Tran Vivian Dang Amin Mohammed Shaik Amruth Chilukurri Berfin Suzer Phil De Vera Miyo Sun Pauline Nguyen Ashley Lee Amirah Salem Joyce Loi Mahmoud Singer Tak Source Type: research
