Clinical Correlation of Function and TCR v β Diversity of MAGE-C2-Specific CD8 < sup > + < /sup > T Cell Response in Esophageal Cancer
In this study, we aimed to explore the immunologic properties of MAGE-C2-specific CD8+ T cells and the relationship of its TCR β-chain V region (TCR vβ) subfamily distribution to prognosis of patients with esophageal cancer. PBMCs and tumor-infiltrating lymphocytes expanded by CD3/CD28 Dynabeads and MAGE-C2 peptides in vitro resulted in the induction of lysosome-associated membrane protein-1 (LAMP-1 or CD107a) on the cell surface and the production of IFN-γ by MAGE-C2-specific CD8+ T cells. We found differential TCR vβ subfamily distribution among flow-sorted CD107a+IFN-γ+ and CD107a-IFN-γ- CD8+ T cells. The proporti...
Source: Journal of Immunology - August 15, 2022 Category: Allergy & Immunology Authors: Pupu Li Xinfeng Chen Yu Ping Guohui Qin Lan Huang Qitai Zhao Zhen Zhang Huanan Chen Liping Wang Shengli Yang Yi Zhang Source Type: research

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J Immunol. 2022 Aug 15;209(4):643. doi: 10.4049/jimmunol.2290015.NO ABSTRACTPMID:35961668 | DOI:10.4049/jimmunol.2290015 (Source: Journal of Immunology)
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Source Type: research

Dietary Cholesterol Metabolite Regulation of Tissue Immune Cell Development and Function
J Immunol. 2022 Aug 15;209(4):645-653. doi: 10.4049/jimmunol.2200273.ABSTRACTObesity is considered the primary environmental factor associated with morbidity and severity of wide-ranging inflammatory disorders. The molecular mechanism linking high-fat or cholesterol diet to imbalances in immune responses, beyond the increased production of generic inflammatory factors, is just beginning to emerge. Diet cholesterol by-products are now known to regulate function and migration of diverse immune cell subsets in tissues. The hydroxylated metabolites of cholesterol oxysterols as central regulators of immune cell positioning in l...
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Authors: Michela Frascoli Andrea Reboldi Joonsoo Kang Source Type: research

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J Immunol. 2022 Aug 15;209(4):643. doi: 10.4049/jimmunol.2290015.NO ABSTRACTPMID:35961668 | DOI:10.4049/jimmunol.2290015 (Source: Journal of Immunology)
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Source Type: research

Dietary Cholesterol Metabolite Regulation of Tissue Immune Cell Development and Function
J Immunol. 2022 Aug 15;209(4):645-653. doi: 10.4049/jimmunol.2200273.ABSTRACTObesity is considered the primary environmental factor associated with morbidity and severity of wide-ranging inflammatory disorders. The molecular mechanism linking high-fat or cholesterol diet to imbalances in immune responses, beyond the increased production of generic inflammatory factors, is just beginning to emerge. Diet cholesterol by-products are now known to regulate function and migration of diverse immune cell subsets in tissues. The hydroxylated metabolites of cholesterol oxysterols as central regulators of immune cell positioning in l...
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Authors: Michela Frascoli Andrea Reboldi Joonsoo Kang Source Type: research

Top Reads
J Immunol. 2022 Aug 15;209(4):643. doi: 10.4049/jimmunol.2290015.NO ABSTRACTPMID:35961668 | DOI:10.4049/jimmunol.2290015 (Source: Journal of Immunology)
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Source Type: research

Dietary Cholesterol Metabolite Regulation of Tissue Immune Cell Development and Function
J Immunol. 2022 Aug 15;209(4):645-653. doi: 10.4049/jimmunol.2200273.ABSTRACTObesity is considered the primary environmental factor associated with morbidity and severity of wide-ranging inflammatory disorders. The molecular mechanism linking high-fat or cholesterol diet to imbalances in immune responses, beyond the increased production of generic inflammatory factors, is just beginning to emerge. Diet cholesterol by-products are now known to regulate function and migration of diverse immune cell subsets in tissues. The hydroxylated metabolites of cholesterol oxysterols as central regulators of immune cell positioning in l...
Source: Journal of Immunology - August 12, 2022 Category: Allergy & Immunology Authors: Michela Frascoli Andrea Reboldi Joonsoo Kang Source Type: research

TAK1 Limits Death Receptor Fas-Induced Proinflammatory Cell Death in Macrophages
J Immunol. 2022 Aug 10:ji2200322. doi: 10.4049/jimmunol.2200322. Online ahead of print.ABSTRACTFas, a member of the death receptor family, plays a central role in initiating cell death, a biological process crucial for immune homeostasis. However, the immunological and pathophysiological impacts to which enhanced Fas signaling gives rise remain to be fully understood. Here we demonstrate that TGF-β-activated kinase 1 (TAK1) works as a negative regulator of Fas signaling in macrophages. Upon Fas engagement with high concentrations of FasL, mouse primary macrophages underwent cell death, and, surprisingly, Fas stimulation l...
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Kengo Maeda Jun Nakayama Shinsuke Taki Hideki Sanjo Source Type: research

A < em > Bcl6 < /em > Intronic Element Regulates T Follicular Helper Cell Differentiation
In this study, we demonstrated that the Bcl6 gene is regulated by FOXO1-binding, cis-acting sequences located in a highly conserved region of the first Bcl6 intron. In both mouse and human T cells, deletion of the tandem FOXO1 binding sites increased the expression of BCL6 and enhanced the proportion of TFH cells. These results reveal a fundamental control point for cellular versus humoral immunity.PMID:35948398 | DOI:10.4049/jimmunol.2100777 (Source: Journal of Immunology)
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Chen-Yen Lai Nimi Marcel Allen W Yaldiko Arnaud Delpoux Stephen M Hedrick Source Type: research

The RAG1 Ubiquitin Ligase Domain Stimulates Recombination of TCR β and TCRα Genes and Influences Development of αβ T Cell Lineages
We report in this study that mice with a mutation that inactivates the Rag1 ubiquitin ligase in vitro exhibit decreased rearrangements and altered repertoires of TCRβ and TCRα genes in thymocytes and impaired thymocyte developmental transitions that require the assembly and selection of functional TCRβ and/or TCRα genes. These Rag1 mutant mice present diminished positive selection and superantigen-mediated negative selection of conventional αβ T cells, decreased genesis of invariant NK T lineage αβ T cells, and mature CD4+ αβ T cells with elevated autoimmune potential. Our findings reveal that the Rag1 ubiquitin ...
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Thomas N Burn Charline Miot Scott M Gordon Erica J Culberson Tamir Diamond Portia A Kreiger Katharina E Hayer Anamika Bhattacharyya Jessica M Jones Craig H Bassing Edward M Behrens Source Type: research

TAK1 Limits Death Receptor Fas-Induced Proinflammatory Cell Death in Macrophages
J Immunol. 2022 Aug 10:ji2200322. doi: 10.4049/jimmunol.2200322. Online ahead of print.ABSTRACTFas, a member of the death receptor family, plays a central role in initiating cell death, a biological process crucial for immune homeostasis. However, the immunological and pathophysiological impacts to which enhanced Fas signaling gives rise remain to be fully understood. Here we demonstrate that TGF-β-activated kinase 1 (TAK1) works as a negative regulator of Fas signaling in macrophages. Upon Fas engagement with high concentrations of FasL, mouse primary macrophages underwent cell death, and, surprisingly, Fas stimulation l...
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Kengo Maeda Jun Nakayama Shinsuke Taki Hideki Sanjo Source Type: research

A < em > Bcl6 < /em > Intronic Element Regulates T Follicular Helper Cell Differentiation
In this study, we demonstrated that the Bcl6 gene is regulated by FOXO1-binding, cis-acting sequences located in a highly conserved region of the first Bcl6 intron. In both mouse and human T cells, deletion of the tandem FOXO1 binding sites increased the expression of BCL6 and enhanced the proportion of TFH cells. These results reveal a fundamental control point for cellular versus humoral immunity.PMID:35948398 | DOI:10.4049/jimmunol.2100777 (Source: Journal of Immunology)
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Chen-Yen Lai Nimi Marcel Allen W Yaldiko Arnaud Delpoux Stephen M Hedrick Source Type: research

The RAG1 Ubiquitin Ligase Domain Stimulates Recombination of TCR β and TCRα Genes and Influences Development of αβ T Cell Lineages
We report in this study that mice with a mutation that inactivates the Rag1 ubiquitin ligase in vitro exhibit decreased rearrangements and altered repertoires of TCRβ and TCRα genes in thymocytes and impaired thymocyte developmental transitions that require the assembly and selection of functional TCRβ and/or TCRα genes. These Rag1 mutant mice present diminished positive selection and superantigen-mediated negative selection of conventional αβ T cells, decreased genesis of invariant NK T lineage αβ T cells, and mature CD4+ αβ T cells with elevated autoimmune potential. Our findings reveal that the Rag1 ubiquitin ...
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Thomas N Burn Charline Miot Scott M Gordon Erica J Culberson Tamir Diamond Portia A Kreiger Katharina E Hayer Anamika Bhattacharyya Jessica M Jones Craig H Bassing Edward M Behrens Source Type: research

TAK1 Limits Death Receptor Fas-Induced Proinflammatory Cell Death in Macrophages
J Immunol. 2022 Aug 10:ji2200322. doi: 10.4049/jimmunol.2200322. Online ahead of print.ABSTRACTFas, a member of the death receptor family, plays a central role in initiating cell death, a biological process crucial for immune homeostasis. However, the immunological and pathophysiological impacts to which enhanced Fas signaling gives rise remain to be fully understood. Here we demonstrate that TGF-β-activated kinase 1 (TAK1) works as a negative regulator of Fas signaling in macrophages. Upon Fas engagement with high concentrations of FasL, mouse primary macrophages underwent cell death, and, surprisingly, Fas stimulation l...
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Kengo Maeda Jun Nakayama Shinsuke Taki Hideki Sanjo Source Type: research

A < em > Bcl6 < /em > Intronic Element Regulates T Follicular Helper Cell Differentiation
In this study, we demonstrated that the Bcl6 gene is regulated by FOXO1-binding, cis-acting sequences located in a highly conserved region of the first Bcl6 intron. In both mouse and human T cells, deletion of the tandem FOXO1 binding sites increased the expression of BCL6 and enhanced the proportion of TFH cells. These results reveal a fundamental control point for cellular versus humoral immunity.PMID:35948398 | DOI:10.4049/jimmunol.2100777 (Source: Journal of Immunology)
Source: Journal of Immunology - August 10, 2022 Category: Allergy & Immunology Authors: Chen-Yen Lai Nimi Marcel Allen W Yaldiko Arnaud Delpoux Stephen M Hedrick Source Type: research