Peripheral CD19+CD24highCD38high B-regulatory cells in lung transplant recipients.
Abstract BACKGROUND: The role of CD19+CD24highCD38high B-regulatory cells in solid-organ Transplant (Tx) in acceptance are still scarce. In previous studies on kidney transplant recipients may suggest a protective role of this cell subtype in graft tolerance and the existence of a cross talk between B-and T-regulatory clones. In lung transplantation, the role of B-regulatory cells has never been investigated. In a murine tracheal transplantation model, this subset seems able to prevent tracheal obliteration when in combination with rapamycin. Aim of this study is to analyze peripheral CD19+CD24highCD38high B-reg c...
Source: Transplant Immunology - September 14, 2019 Category: Transplant Surgery Authors: Davide P, Monica M, Sara M, Alice B, Simona I, Emanuela C, Oggionni T, Vanessa F, Laura P, Luigia S, Federica M Tags: Transpl Immunol Source Type: research

FK506 combined with GM6001 prevents tracheal obliteration in a mouse model of heterotopic tracheal transplantation.
In this study, we investigated the effect of tacrolimus (FK506) combined with GM6001,a matrix metalloproteinase (MMP) inhibitor, on the formation of OB using a mouse heterotopic tracheal transplantation model. METHODS: Syngeneic tracheal grafts were transplanted heterotopically from BALB/c mice to BALB/c mice. Allografts from C57BL/6 mice were transplanted to BALB/c mice. Isograft group, allograft group, allograft+FK506 group, allograft +GM6001 group and allograft+FK506 + GM6001 group was given respectively intraperitoneal injection of saline, saline, FK506, GM6001 and FK506 + GM6001 once a day. At 28 da...
Source: Transplant Immunology - September 14, 2019 Category: Transplant Surgery Authors: Li Y, Shu P, Tang L, Yang X, Fan J, Zhang X Tags: Transpl Immunol Source Type: research

The effect of a novel immunosuppressive drug, a PAK-2 inhibitor, on macrophage differentiation/polarization in a rat small intestinal transplantation model.
In this study, we investigated the function of this drug mainly on macrophages using a rat small intestinal transplant model. METHODS: Male Dark Agouti (DA) and Lewis rats (LEW), 7-9 weeks of age, were used as donor and recipient, respectively. Approximately 15 cm intestinal grafts were heterotopically transplanted to the recipient rats. The recipient rat was treated with PQA-18 (4 mg/kg/per day) by intraperitoneal injection (ip) from postoperative day 1 for 2 weeks. The in vivo effects of this drug were evaluated based on changes in body weight, and the population of each type of blood cell. Mixed lymphocyte ...
Source: Transplant Immunology - September 14, 2019 Category: Transplant Surgery Authors: Kodama T, Maeda A, Lo PC, Noguchi Y, Toyama C, Takama Y, Ueno T, Tazuke Y, Eguchi H, Matsunami K, Miyagawa S, Okuyama H Tags: Transpl Immunol Source Type: research

Human CTLA4-Ig therapy can give false-positive anti-pig antibody results in primates after xenotransplantation.
Abstract BACKGROUND: Measurement of serum anti-pig antibodies is an important parameter in immune monitoring after pig-to-nonhuman primate xenotransplantation. Pig aortic endothelial cells (pAECs) are commonly used for this purpose. However, human (h) CTLA4-Ig (abatacept/belatacept) could bind to pCD80/86 on the cells, and a secondary antibody (i.e., anti-human IgG) may recognize hCTLA4-Ig (in the absence of serum anti-pig IgG antibody binding to pAECs), potentially leading to misinterpretation of the results. Our aim was to determine whether hCTLA4-Ig binding to pAECs is associated with false-positive results. ...
Source: Transplant Immunology - August 28, 2019 Category: Transplant Surgery Authors: Li Q, Yamamoto T, Iwase H, Nguyen HQ, Wang Y, Ayares D, Cooper DKC, Hara H Tags: Transpl Immunol Source Type: research

The effect of oral melatonin on renal ischemia-reperfusion injury in transplant patients: A double-blind, randomized controlled trial.
Abstract BACKGROUND: One of the important factors in the occurrence of acute kidney injury (AKI) among renal transplant patients (RTPs) is ischemia reperfusion injury (IRI). The current study aimed at determining the anti-inflammatory and anti-oxidative effects of melatonin on the complications of IRI and the level of Klotho expression in these patients. METHODS: A total of 40 renal transplant candidates were randomly assigned into placebo or melatonin group receiving the same dose of 3 mg/day. In order to measure serum melatonin levels, inflammatory and oxidative stress factors, renal function biomarkers, ...
Source: Transplant Immunology - August 22, 2019 Category: Transplant Surgery Authors: Panah F, Ghorbanihaghjo A, Argani H, Haiaty S, Rashtchizadeh N, Hosseini L, Dastmalchi S, Rezaeian R, Alirezaei A, Jabarpour M, Ahmad SNS, Sanajoo D Tags: Transpl Immunol Source Type: research

Negative impact of recipient SPRED2 deficiency on transplanted lung in a mouse model.
Abstract Ischemia-reperfusion injury (IRI) after lung transplantation mainly contributes to the development of primary graft dysfunction. The Sprouty-related EVH1-domain-containing (SPRED) protein family inhibits the mitogen activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) pathway. Our study was aimed at examining the role of SPRED2 in IRI in mice that received orthotopic lung transplantation. Syngeneic mouse lung transplantation was performed in wild-type C57BL/6 J (WT) mice and Spred2 knockout (Spred2-/-) mice on the C57BL/6 J background from the WT donor. Four hours after reperfusio...
Source: Transplant Immunology - August 22, 2019 Category: Transplant Surgery Authors: Hashimoto K, Yamane M, Sugimoto S, Hirano Y, Kurosaki T, Otani S, Miyoshi K, Ohara T, Okazaki M, Yoshimura T, Oto T, Matsukawa A, Toyooka S Tags: Transpl Immunol Source Type: research

Prognostic value of C3d-fixing, preformed donor-specific antibodies in crossmatch-positive living kidney transplantation.
Abstract The occurrence of acute antibody-mediated rejection (ABMR) is higher in flow cytometric crossmatch (FCXM)-positive patients despite desensitization. Accumulating evidence suggests a correlation between the complement-binding ability of donor-specific antibodies (DSAs) and the risk of ABMR. Here, we investigated the correlation between complement C3d-fixing ability of preformed DSA and ABMR risk, the efficacy of a desensitization protocol for patients with C3d-fixing DSA, and the risk of ABMR in 21 DSA- and FCXM-positive patients. We retrospectively analyzed the C3d-fixing ability and mean fluorescence int...
Source: Transplant Immunology - August 6, 2019 Category: Transplant Surgery Authors: Miyake K, Okumi M, Kakuta Y, Unagami K, Furusawa M, Ishida H, Tanabe K Tags: Transpl Immunol Source Type: research

Immunoglobulin abnormalities in 1677 solid organ transplant recipients. Implications for posttransplantation follow-up.
CONCLUSIONS: GP after SOT is associated with a high risk of PTLD, allograft loss and poor survival. The combination of SPE, SIFE, SFLC and UIFE is optimal for GP detection. These methods aid in identifying patients who are at risk for PTLD or allograft damage and should be included in regular post-Tx follow-up. PMID: 31398462 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - August 6, 2019 Category: Transplant Surgery Authors: Sečník P, Jabor A, Lavríková P, Sečník J, Malíková P, Pokorná E, Franeková J Tags: Transpl Immunol Source Type: research

Suppressor of cytokine signaling genes in renal transplant receivers: Association with transplant fate.
Abstract Suppressor of cytokine signaling (SOCS) proteins have acknowledged roles in regulation of immune responses. Moreover, their role in the evolution of allograft rejection is being elucidated. In the current investigation, we measured transcript levels of SOCS1-4 in the peripheral blood of a group of renal transplant recipients including both rejected and non-rejected allografts. Expression analyses showed that relative expression of SOCS2 was significantly higher in transplant-rejected male patients compared to non-rejected group. However, such significant difference was not detected between female subjects...
Source: Transplant Immunology - August 6, 2019 Category: Transplant Surgery Authors: Nafar M, Kalantari S, Omrani MD, Samavat S, Arsang-Jang S, Taheri M, Ghafouri-Fard S Tags: Transpl Immunol Source Type: research

Tacrolimus exposure after liver transplantation for alcohol-related liver disease: Impact on complications.
Abstract BACKGROUND: Alcohol-related liver disease (ALD) is one of the main indications for liver transplantation (LT). For 20 years, tacrolimus (Tac) is the cornerstone immunosuppressive drug used after LT and is very efficient for the prevention of rejection. Nevertheless, the major drawback of long-term use of Tac is the risk for developing dose-dependent adverse effects. OBJECTIVE: The aim of the present study was to assess the impact of Tac exposure (trough concentrations and concentration/dose (C/D) ratio) during the first year after LT, on short- and long-term complications after LT for ALD. MET...
Source: Transplant Immunology - July 24, 2019 Category: Transplant Surgery Authors: Bardou FN, Guillaud O, Erard-Poinsot D, Chambon-Augoyard C, Thimonier E, Vallin M, Boillot O, Dumortier J Tags: Transpl Immunol Source Type: research

Low dose valganciclovir as cytomegalovirus prophylaxis in post-renal transplant recipients induced with alemtuzumab: A single-center study.
CONCLUSION: In this institution's experience, CMV incidence was reduced compared to historically reported data by using low-dose (450 mg) VGCV prophylaxis in combination with Ale induction and steroid minimization. However, overall rejection rate was significantly higher in our population, possibly influenced by the degree of steroid minimization. PMID: 31344441 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - July 22, 2019 Category: Transplant Surgery Authors: Korneffel K, Mitro G, Buschor K, Rees M, Ortiz J Tags: Transpl Immunol Source Type: research

Preliminary assessment of the feasibility of autologous myeloid-derived suppressor cell infusion in non-human primate kidney transplantation.
Abstract Myeloid-derived suppressor cells (MDSC) are a heterogenous population of immunosuppressive myeloid cells now considered important immune regulatory cells in diverse clinical conditions, including cancer, chronic inflammatory disorders and transplantation. In rodents, MDSC administration can inhibit graft-versus-host disease lethality and enhance organ or pancreatic islet allograft survival. There is also evidence, however, that under systemic inflammatory conditions, adoptively-transferred MDSC can rapidly lose their suppressive function. To our knowledge, there are no reports of autologous MDSC administr...
Source: Transplant Immunology - July 19, 2019 Category: Transplant Surgery Authors: Ezzelarab MB, Perez-Gutierrez A, Humar A, Wijkstrom M, Zahorchak AF, Lu-Casto L, Wang YC, Wiseman RW, Minervini M, Thomson AW Tags: Transpl Immunol Source Type: research

Collagen type-V is a danger signal associated with primary graft dysfunction in lung transplantation.
CONCLUSIONS: This study demonstrated that col.(V) is an rapidly recognized by B cells and has specific transcriptional signature. In lung transplants recipients the rapid seroconversion to anti-col(V) Ab is linked to increased risk of grade 3 PGD. PMID: 31325493 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - July 17, 2019 Category: Transplant Surgery Authors: Zaffiri L, Shah RJ, Stearman RS, Rothhaar K, Emtiazjoo AM, Yoshimoto M, Fisher AJ, Mickler EA, Gartenhaus MD, Cohort LTOG, Diamond JM, Geraci MW, Christie JD, Wilkes DS Tags: Transpl Immunol Source Type: research

Banff lesions and renal allograft survival in chronic-active antibody mediated rejection.
CONCLUSION: Severity of renal interstitial fibrosis and not inflammation predicts graft survival in cases of c-aABMR. PMID: 31279831 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - July 4, 2019 Category: Transplant Surgery Authors: Sablik KA, Clahsen-van Groningen MC, Damman J, Roelen DL, Betjes MGH Tags: Transpl Immunol Source Type: research

Late impact of preformed anti-HLA antibodies on kidney graft outcome.
PMID: 31254612 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - June 26, 2019 Category: Transplant Surgery Authors: da Silva CK, Meinerz G, Bruno RM, Abud J, Montagner J, Dorsdt DMB, Coutinho AK, Neumann J, Garcia VD, Keitel E Tags: Transpl Immunol Source Type: research

Mfn2 inhibits chronic rejection of the rat abdominal aorta by regulating TGF- β1 levels.
CONCLUSION: Mfn2 influences TGF-β/smad pathway and may function as potential chronic rejection inhibitor. PMID: 31247293 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - June 24, 2019 Category: Transplant Surgery Authors: Sun Q, Chen L, Zhou D, Yang Q, Song W, Chen D, Wang W Tags: Transpl Immunol Source Type: research

Circulating microvesicle protein is associated with renal transplant outcome.
Abstract Renal transplantation is an effective therapy with improved long-term outcomes compared with other therapies for end stage renal disease. Present methods for evaluating kidney allograft function, such as serum creatinine or allograft biopsy, are not sensitive and identify pathological changes only after any potential intervention would be effective. Thus, there is a necessity for biomarkers that would provide early prognostic information about kidney transplant outcomes. Circulating microvesicles represent an attractive source of biomarkers for different diseases including renal failure. We have studied t...
Source: Transplant Immunology - June 18, 2019 Category: Transplant Surgery Authors: Al-Nedawi K, Haas-Neil S, Gangji A, Ribic CM, Kapoor A, Margetts P Tags: Transpl Immunol Source Type: research

Impact of alemtuzumab pharmacokinetics on T-cell dynamics, graft-versus-host disease and viral reactivation in patients receiving allogeneic stem cell transplantation with an alemtuzumab-based T-cell-depleted graft.
Abstract Administration of alemtuzumab (targeting the CD52 antigen) to the patient (in-vivo) or to the graft (in-vitro) before allogeneic stem cell transplantation (alloSCT) decreases the incidence of graft-versus-host disease (GvHD). Effectiveness of this treatment relies on depletion of donor T cells. Currently, no data are available on alemtuzumab pharmacokinetics and pharmacodynamics in patients who received combined in-vivo and in-vitro alemtuzumab-based T-cell depletion. In this prospective study, we analyzed alemtuzumab pharmacokinetics and its effect on the circulating T cells in 36 patients who received a...
Source: Transplant Immunology - June 14, 2019 Category: Transplant Surgery Authors: Loeff FC, van Egmond EHM, Moes DJAR, Wijnands C, Von Dem Borne PA, Veelken H, Falkenburg JHF, Jedema I, Halkes CJM Tags: Transpl Immunol Source Type: research

Immunological profiles of HIV-positive recipients of liver transplant.
CONCLUSIONS: Immune cell subpopulations potentially associated with HIV progression and organ rejection were detected in HIV-positive transplant recipients. We confirmed altered frequencies of B, T, and NK cell populations in HIV-positive liver transplant recipients compared to healthy controls. The imbalance among immune cell subsets deserves further studies to identify markers of transplant outcome and potential therapeutic targets. PMID: 31102654 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - May 15, 2019 Category: Transplant Surgery Authors: Righi E, Ivaldi F, La Rosa A, Carnelutti A, Londero A, Bassetti M Tags: Transpl Immunol Source Type: research

Switching renal transplant recipients to belatacept therapy results of a real-life gradual conversion protocol.
Abstract Conversion to belatacept immunosuppression is a therapeutic option for renal-transplant recipients with calcineurin inhibitors (CNI) toxicity, but it associates with high risk of acute rejection. Gradual conversion and serial immune monitoring with urinary chemokine CXCL9 may allow increasing safety of this maneuver. We converted kidney transplant recipients with signs of toxicity to CNI or other immunosuppressive drugs to belatacept over a 2-month period. We monitored renal function, metabolic profile, and circulating lymphocyte subsets. We also quantified urinary CXCL9 over a 12-month follow-up period. ...
Source: Transplant Immunology - May 6, 2019 Category: Transplant Surgery Authors: Malvezzi P, Fischman C, Rigault G, Jacob MC, Raskovalova T, Jouve T, Janbon B, Rostaing L, Cravedi P Tags: Transpl Immunol Source Type: research

Analysis of genetic and clinical risk factors of post-transplant thrombocytopenia in kidney allograft recipients.
In this study, posttransplant thrombocytopenia was defined as a 30% reduction in platelet count from the baseline in the first week or a decrease of
Source: Transplant Immunology - April 19, 2019 Category: Transplant Surgery Authors: Choi KH, Chang Y, Shah T, Min DI Tags: Transpl Immunol Source Type: research

Adipose tissue-derived mesenchymal stem cells ameliorate bone marrow aplasia related with graft-versus-host disease in experimental murine models.
This study is the first to clarify the effectiveness of AD-MSCs against bone marrow aplasia in GVHD, supporting a rationale of AD-MSCs for ameliorating bone marrow suppression and infectivity after allo-HSCT in human clinics. PMID: 30946889 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - April 1, 2019 Category: Transplant Surgery Authors: Nishi Y, Murakami A, Murayama Y, Tsukahara N, Okamoto S, Nakachi S, Morichika K, Tamaki K, Noguchi H, Matsushita M, Karube KN, Fukushima T, Morishima S, Kishimoto H, Masuzaki H Tags: Transpl Immunol Source Type: research

Salvianolic acid B ameliorates liver injury in a murine aGvHD model by decreasing inflammatory responses via upregulation of HO-1.
Abstract Acute graft-versus-host disease (aGvHD) remains lethal, even after allogeneic hematopoietic stem cell transplantation. Inflammatory responses play an important role in aGvHD. Salvianolic acid B (Sal B) has been widely reported to have a major effect on the anti-inflammatory response, but these effects in an aGvHD model have never been reported. B6 donor splenocytes were transplanted into unirradiated BDF1 recipients and liver and serum were collected on day 14 after transplantation with or without Sal B administration. We measured the expression of pro-inflammatory cytokines and chemokines and other manif...
Source: Transplant Immunology - March 20, 2019 Category: Transplant Surgery Authors: Zhao J, Yang XC, Fujino M, Ichimaru N, Que W, Li XK, Takahara S Tags: Transpl Immunol Source Type: research

Endowing human CD8 T cells with a veto-like recognition capacity via the electroporation of MHC-I/CD3 ζ mRNA.
This study paves the way for further advancing this approach as a means to dampen GVHD and HVG that are caused by HLA disparity in allo-HSCT. PMID: 30904624 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - March 20, 2019 Category: Transplant Surgery Authors: Weissberg O, Gross G Tags: Transpl Immunol Source Type: research

Recurrent acute cellular rejection graded ISHLT 1R early after heart transplantation negatively affects long-term outcomes: The prognostic significance of 1990 ISHLT grades 1B and 2.
Abstract PURPOSE: We investigated the implications of early recurrent 1R rejections for long-term outcomes after heart transplantation (HT) and evaluated the prognostic significance of 1990 ISHLT grading 1B/2 versus 1A. METHODS: Data on all patients who underwent HT between 1992 and 2017 were reviewed. Patients with ≥2 endomyocardial biopsies graded 1R in the first 3 months were classified as "recurrent 1R." Those patients were further categorized according to 1A vs. 1B/2. Outcomes (>3 months) were long-term rejections and the combined endpoint of cardiac allograft vasculopathy (CAV) and c...
Source: Transplant Immunology - March 20, 2019 Category: Transplant Surgery Authors: Peled Y, Lavee J, Ram E, Kassif Y, Peled A, Freimark D, Ofek E, Kogan A Tags: Transpl Immunol Source Type: research

Prevalence of antibodies to lung self-antigens (K α1 tubulin and collagen V) and donor specific antibodies to HLA in lung transplant recipients and implications for lung transplant outcomes: Single center experience.
CONCLUSIONS: In our cohort, we observed a high prevalence of autoantibodies and DSA in lung transplant recipients. Pre-transplant autoantibodies were associated with de novo development of DSA along with a decrease in BOS-free survival. Limitations to our study include the small sample size and single center enrollment, along with limited time for follow-up. PMID: 30794945 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - February 19, 2019 Category: Transplant Surgery Authors: Rao U, Sharma M, Mohanakumar T, Ahn C, Gao A, Kaza V Tags: Transpl Immunol Source Type: research

Increased CD16 expression on NK cells is indicative of antibody-dependent cell-mediated cytotoxicity in chronic-active antibody-mediated rejection.
Abstract Chronic-active antibody mediated rejection (c-aABMR) contributes significantly to late renal allograft failure. The antibodies directed against donor-derived antigens, e.g. anti-HLA antibodies, cause inflammation at the level of the microvascular endothelium. This is characterized by signs of local activation of the complement system and accumulation of immune cells within the capillaries. Non-invasive biomarkers of c-aABMR are currently not available but could be valuable for early detection. We therefore analyzed the activation profiles of circulating T and B cells, NK cells and monocytes in the periphe...
Source: Transplant Immunology - February 19, 2019 Category: Transplant Surgery Authors: Sablik KA, Litjens NHR, Klepper M, Betjes MGH Tags: Transpl Immunol Source Type: research

VEGF-C attenuates ischemia reperfusion injury of liver graft in rats.
CONCLUSION: Exogenous VEGF-C protects liver graft from IRI by regulating the inflammatory response and modifying polarization of KCs. PMID: 30771456 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - February 13, 2019 Category: Transplant Surgery Authors: Cheng MX, Li JZ, Chen Y, Cao D, Gong JP, Tu B Tags: Transpl Immunol Source Type: research

Pregnancy and donor-specific HLA-antibody-mediated rejection after liver transplantation: "Liaisons dangereuses"?
CONCLUSION: The results of the present study suggest that close monitoring of DSA in young women with history of pregnancy should be recommended regarding the risk of DSA-mediated rejection. PMID: 30743001 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - February 8, 2019 Category: Transplant Surgery Authors: Dumortier J, Dedic T, Erard-Poinsot D, Rivet C, Guillaud O, Chambon-Augoyard C, Bosch A, Lachaux A, Couchonnal E, Thaunat O, Boillot O, Dubois V Tags: Transpl Immunol Source Type: research

Increased splenic human CD4+:CD8+ T cell ratios, serum human interferon- γ and intestinal human interleukin-17 are associated with clinical graft-versus-host disease in humanized mice.
This study also provides further insights into GVHD development which may be relevant to human HSCT recipients. PMID: 30743002 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - February 8, 2019 Category: Transplant Surgery Authors: Geraghty NJ, Belfiore L, Adhikary SR, Alexander SI, Sluyter R, Watson D Tags: Transpl Immunol Source Type: research

Ethylene carbodiimide-fixed donor splenocytes combined with cordycepin induce long-term protection to mice cardiac allografts.
In this study, we aimed to determine the effect of ECDI-SPs combined with a short course of cordycepin (an anti-inflammatory agent) on the long-term outcomes of mice cardiac allografts. Our results demonstrated that ECDI-SPs combined with cordycepin significantly promoted mice cardiac allograft survival compared with ECDI-SPs monotherapy. This effect was accompanied by decreased production of pro-inflammatory cytokines (IL-1β, IL-6, IL-17 and TNFα), increased secretion of anti-inflammatory cytokines (IL-10 and TGFβ), inhibition of Th17 and expansion of Tregs, and prevention of I/R injury. We concluded that ...
Source: Transplant Immunology - February 8, 2019 Category: Transplant Surgery Authors: Xingqiang L, Fen N, Zhongpeng Y, Tiantian W, Lei Z, Jiali F, Junjie M, Guanghui L, Lu X, Yuhe G, Zheng C Tags: Transpl Immunol Source Type: research

Evaluation of the host immune response and functional recovery in peripheral nerve autografts and allografts.
In conclusion, in PN autografts the immune system is synchronic initiating with a more robust early innate response that more rapidly transitions to adaptive while for PN allografts the infiltration of immune cells is slower and more gradually progresses to a moderate adaptive response. PMID: 30735701 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - February 5, 2019 Category: Transplant Surgery Authors: Roballo KCS, Bushman J Tags: Transpl Immunol Source Type: research

Mannose binding lectin 2 gene polymorphisms in patients after renal transplantation with acute graft rejection.
This study enrolled 266 Caucasian deceased-donor renal transplant recipients - 69 with diagnosed acute graft rejection, 197 with stable graft function. A 969 bp DNA fragment, from chromosome 10, including promoter region and exon 1 of MBL2 gene was sequenced. The DNA fragment obtained contained 122 SNPs accordingly to the NCBI dbSNP database. Of this number only nine showed variation within our population (rs36014597, rs5030737, rs1800450, rs7095891, rs11003123, rs7096206, rs7084554, rs11003124, rs11003125), and only these were subjected to further analysis. Among the studied polymorphisms in the MBL gene only rs1800450 po...
Source: Transplant Immunology - January 30, 2019 Category: Transplant Surgery Authors: Czerewaty M, Tarnowski M, Safranow K, Domanski L, Pawlik A Tags: Transpl Immunol Source Type: research

Protective effect of rosiglitazone on chronic renal allograft dysfunction in rats.
CONCLUSIONS: Rosiglitazone successfully attenuates the development of CRAD via inhibition of TGF-β signaling, the renal tubular epithelial-to-mesenchymal transition (EMT), and inflammation. PMID: 30682409 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - January 22, 2019 Category: Transplant Surgery Authors: Deng J, Xia Y, Zhou Q, Wang X, Xiong C, Shao X, Shao M, Zou H Tags: Transpl Immunol Source Type: research

Kinetics of antibody response to influenza vaccination in renal transplant recipients.
In this study, we investigated the kinetics of vaccine strains-specific antibody responses to trivalent influenza vaccine in a group of renal transplant recipients and a control group. First, we found that the geometric mean hemagglutination inhibition titer against all 3 vaccine strains in the transplant cohort was significantly low when compared to control subjects. Next, whereas the control group sera showed significantly higher HA-specific IgG and isotype IgG1 antibodies at all four time points, a similar increase in the transplant group was delayed until day 28. Interestingly, within the transplant group, subjects rec...
Source: Transplant Immunology - January 18, 2019 Category: Transplant Surgery Authors: Gangappa S, Wrammert J, Wang D, Li ZN, Liepkalns JS, Cao W, Chen J, Levine MZ, Stevens J, Sambhara S, Begley B, Mehta A, Pearson TC, Ahmed R, Larsen CP Tags: Transpl Immunol Source Type: research

Jon van Rood: The pioneer and his personal view on the early developments of HLA and immunogenetics.
Abstract A single observation in a patient with an unusual transfusion reaction led to a life-long fascination with immunogenetics, and a strong wish to improve the care for patients needing a transplantation. In 2017 Jon van Rood, one of the pioneers in the field of HLA and immunogenetics of transplantation, passed away. Several obituaries have appeared describing some of the highlights of his career. However, the details of the early developments leading among others to the routine use of HLA as an important parameter for donor selection in organ- and hematopoietic stem cell transplantation are largely unknown t...
Source: Transplant Immunology - January 3, 2019 Category: Transplant Surgery Authors: Jager MJ, Brand A, Claas FHJ Tags: Transpl Immunol Source Type: research

Can the Toll-like receptors 4 expression in peripheral blood mononuclear cells help assess the effectiveness of immunosuppression and the chance of a future good renal transplant function?
CONCLUSION: In kidney transplant recipients the occurrence of DGF results in a long-term reduction of the averaged TLR4ex in PBMC. Tacrolimus exerts a clear, stabilizing, positive and dose-dependent effect on TLR4ex. An improvement in renal transplant function may be expected in KT patients with high TLR4ex. Evaluation of the averaged TLR4ex can be used to assess the efficacy of immunosuppression in the treatment with tacrolimus and to estimate the likelihood of deterioration in renal function. PMID: 30592973 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - December 25, 2018 Category: Transplant Surgery Authors: Zmonarski SC, Madziarska K, Golebiowski T, Banasik M, Mazanowska O, Madziarski M, Krajewska M Tags: Transpl Immunol Source Type: research

Tri-allelic expression of HLA gene in 46,XX/46,XY chimerism.
DISCUSSION: A case of normal phenotype 46,XX/46,XY chimerism was detected for the first time in Korean patient admitted for lung transplantation. Cytogenetic results were confirmatory for chimerism and HLA typing using PCR-SBT method was able to detect the presence of 3 HLA alleles. Flowcytometry crossmatch was proven sensitive for detecting antigen expression of different cell lines of small proportions. PMID: 30579837 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - December 20, 2018 Category: Transplant Surgery Authors: Yang JJ, Hwang SH, Ko DH, Seo EJ, Oh HB Tags: Transpl Immunol Source Type: research

Detection of engraftment of donor-derived antibody producing cells in a lung transplant recipient by anti-cytomegalovirus IgG avidity test.
Abstract Transplant recipients become immunocompromised through the use of immunosuppressive therapy to prevent allograft rejection. These recipients readily experience human cytomegalovirus (CMV) infection or reactivation. Therefore, CMV represents a life-threatening pathogen in transplant recipients. To demonstrate the serostatus and course of IgG maturation against CMV in transplant patients, we measured the transition of anti-CMV IgG and its affinity (avidity index; AI) as criteria for antibody maturation. Among 31 lung transplant recipients, 26 were infected with CMV before transplantation and maintained anti...
Source: Transplant Immunology - December 12, 2018 Category: Transplant Surgery Authors: Koshizuka T, Matsuda Y, Suzuki H, Kanno R, Ikuta K, Kobayashi T, Kondo T, Okada Y, Suzutani T Tags: Transpl Immunol Source Type: research

The prevention of tracheal graft occlusion using pioglitazone: A mouse tracheal transplant model study.
Abstract Bronchiolitis obliterans (BO), which begins with lymphocytic bronchiolitis (LB), is the most serious manifestations of chronic rejection following lung transplantation. We have evaluated the effects of intraperitoneal administration of pioglitazone, a peroxisome proliferator-activated receptor (PPAR) gamma agonist, on LB in mice tracheal transplant model. Tracheal grafts from BALB/c or C57BL/6 were transplanted to C57BL/6 recipients. Animals were divided into three groups (n = 8), isograft (IG) and allograft with (AGP) or without (AGN) pioglitazone. Occlusion rate (OR) of transplanted trachea, CD3, Fo...
Source: Transplant Immunology - December 10, 2018 Category: Transplant Surgery Authors: Shigenobu T, Ohtsuka T, Shimoda M Tags: Transpl Immunol Source Type: research

Effects of recombinant thrombomodulin therapy and soluble human leukocyte antigen-G levels during hematopoietic stem cell transplantation.
CONCLUSION: These data suggest that HLA-G/sHLA-G participate in prevention of GVHD, and that rTM may prevent aGVHD following HSCT by promoting elevation of sHLA-G. PMID: 30543860 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - December 10, 2018 Category: Transplant Surgery Authors: Nomura S, Ito T, Katayama Y, Ota S, Hayashi K, Fujita S, Satake A, Ishii K Tags: Transpl Immunol Source Type: research

HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.
CONCLUSIONS: HMGB1 may be a trigger of the BOS pathogenesis and candidate target for the treatment of the disease. PMID: 30508580 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - November 30, 2018 Category: Transplant Surgery Authors: Takamori S, Shoji F, Okamoto T, Kozuma Y, Matsubara T, Haratake N, Akamine T, Katsura M, Takada K, Toyokawa G, Tagawa T, Maehara Y Tags: Transpl Immunol Source Type: research

A novel MyD88 inhibitor attenuates allograft rejection after heterotopic tracheal transplantation in mice.
CONCLUSIONS: In heterotopic tracheal transplantation models, TJ-M2010-5 combined with MR-1 could ameliorate the development of OB. PMID: 30472390 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - November 22, 2018 Category: Transplant Surgery Authors: Yang M, Chen G, Zhang X, Ding Z, Miao Y, Yang Y, Chang S, Zhou P Tags: Transpl Immunol Source Type: research

iTRAQ-based quantitative proteomic analysis reveals potential early diagnostic markers in serum of acute cellular rejection after liver transplantation.
Abstract Liver transplantation (LT) is the most effective treatment method for advanced stage liver disease but acute cellular rejection (ACR) seriously affects the prognosis of LT. To discover novel diagnostic biomarkers of ACR after LT, Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-based mass spectrometry was performed to characterize alterations of serum proteins among patients validated to be pathologically ACR or pathologically no-ACR after LT and healthy controls. As a result, 10 differentially expressed proteins were found out between the ACR group and the No-ACR group; 88 differentially expr...
Source: Transplant Immunology - November 22, 2018 Category: Transplant Surgery Authors: Jiang Q, Ru Y, Yu Y, Li K, Jing Y, Wang J, Li G Tags: Transpl Immunol Source Type: research

An enigmatic case of undiagnosed severe diarrhoea post living donor liver transplant.
We describe a case of severe diarrhoea post living donor OLT who was diagnosed with acute GVHD. Addition of oral budesonide therapy to systemic corticosteroid therapy controlled the symptoms of diarrhoea. PMID: 30472392 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - November 22, 2018 Category: Transplant Surgery Authors: Sonavane A, Saigal S Tags: Transpl Immunol Source Type: research

Anti-perlecan antibodies and acute humoral rejection in hypersensitized patients without forbidden HLA specificities after kidney transplantation.
CONCLUSIONS: The incidence of non-HLA antibodies in patients in the waiting list is largely underestimated. The concomitance anti-HLA and non-HLA Abs in hypersensitized patients is very common and the detection of non-HLA Abs in this population could allow to identify patients with an increased risk of humoral rejection. PMID: 30458294 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - November 17, 2018 Category: Transplant Surgery Authors: Riesco L, Irure J, Rodrigo E, Guiral S, Ruiz JC, Gómez J, López-Hoyos M, Segundo DS Tags: Transpl Immunol Source Type: research

Regulatory effects of dermal papillary pluripotent stem cells on polarization of macrophages from M1 to M2 phenotype in vitro.
Abstract The M1:M2 macrophage ratio is important for spinal cord injury (SCI) repair. Bone marrow mesenchymal stem cells (BMSCs) can alter macrophage activation, promoting M1 to M2 macrophage conversion and SCI repair; however, clinical BMSC applications have limitations. Previously, we found DPCs to be superior to BMSCs in promoting tissue repair after SCI, which we hypothesized to be mediated by M1 to M2 macrophage conversion. We investigated the regulatory effect of DPCs on M1/M2 macrophage polarization. Dermal papilla cells (DPCs) were isolated from rat vibrissae and characterized. Bone marrow-derived macropha...
Source: Transplant Immunology - November 17, 2018 Category: Transplant Surgery Authors: Li M, Xu J, Mei X, Chi G, Li L, Song Y, He X, Li Y Tags: Transpl Immunol Source Type: research

Clinical use of an immune monitoring panel in liver transplant recipients: A prospective, observational study.
Abstract Immunosuppressive therapy greatly contributed to making liver transplantation the standard treatment for end-stage liver diseases. However, it remains difficult to predict and measure the efficacy of pharmacological immunosuppression. Therefore, we used a panel of standardized, commonly available, biomarkers with the aim to describe their changes in the first 3 weeks after the transplant procedure and assess if they may help therapeutic drug monitoring in better tailoring the dose of the immunosuppressive drugs. We prospectively studied 72 consecutive patients from the day of liver transplant (post-oper...
Source: Transplant Immunology - November 7, 2018 Category: Transplant Surgery Authors: Iovino L, Taddei R, Bindi ML, Morganti R, Ghinolfi D, Petrini M, Biancofiore G Tags: Transpl Immunol Source Type: research

Sequential monitoring of TIM-3 mRNA expression in blood and urine samples of renal transplant recipients.
CONCLUSION: Our data suggested that serial monitoring of TIM-3 mRNA level in the blood and urine samples of renal transplant recipients could be a useful non-invasive biomarker for prediction and diagnosis of allograft dysfunction. PMID: 30395925 [PubMed - as supplied by publisher] (Source: Transplant Immunology)
Source: Transplant Immunology - November 2, 2018 Category: Transplant Surgery Authors: Shahbaz SK, Barabadi M, Ahmadpour P, Pourrezagholi F, Nafar M, Foroughi F, Hosseinzadeh M, Ghorbanpour M, Yekaninejad MS, Amirzargar A Tags: Transpl Immunol Source Type: research

Changes in serum KL-6 levels are associated with the development of chronic lung allograft dysfunction in lung transplant recipients.
This study investigated whether changes in serum KL-6 levels over time were associated with CLAD. Twenty-one lung transplant recipients had serum KL-6 measured (NANOPIA®) at baseline and after 7 years. Changes in serum KL-6 levels from baseline were determined. Receiver operating characteristic curves and Kaplan-Meier analysis were used to test the predictive value of changes in serum KL-6 over time. The average increase in KL-6 in patients with CLAD was 15% versus a 28% decrease in non-CLAD patients (p = .042). An 11% decrease in serum KL-6 level was determined as the best cut-off value to be associated with the...
Source: Transplant Immunology - October 31, 2018 Category: Transplant Surgery Authors: Bessa V, Bonella F, Ohshimo S, Weinreich G, Kleibrink B, Costabel U, Aigner C, Kamler M, Taube C, Sommerwerck U Tags: Transpl Immunol Source Type: research