Functional implications of NHR-210 enrichment in C. elegans cephalic sheath glia: insights into metabolic and mitochondrial disruptions in Parkinson's disease models
In conclusion, the deficiency of the nuclear hormone receptor nhr-210 in alpha-synuclein expressing strain of C. elegans, results in altered mitochondrial function, coupled with alterations in vital metabolite levels. These findings underline the functional and physiological importance of nhr-210 enrichment in CEPsh glia.PMID:38691171 | DOI:10.1007/s00018-024-05179-2 (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - May 1, 2024 Category: Cytology Authors: Rohil Hameed Anam Naseer Ankit Saxena Mahmood Akbar Pranoy Toppo Arunabh Sarkar Sanjeev K Shukla Aamir Nazir Source Type: research
Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research
Biotechniques. 2024 May 1. doi: 10.2144/btn-2023-0112. Online ahead of print.ABSTRACTBiobanks of cervical screening (LBC) samples annotated with disease status are an invaluable resource to support the development of tools for the risk stratification of disease. Although there is growing interest in the assessment of RNA-based biomarkers, little is known on the suitability and durability of stored clinical samples (commonly used in cervical screening) to support RNA-based research. RNA was extracted from 260 stored LBC samples. Storage at -80°C or -25°C allowed isolation of sufficient RNA for further analysis. RNA was fo...
Source: BioTechniques - May 1, 2024 Category: Biotechnology Authors: Madeleine P J White Andrew Stevenson Hana Elasifer Chris Davies Kyriaki Nomikou Kate Cuschieri Sheila V Graham Source Type: research
The ASCB and MBoC: Our cell biology power couple
Mol Biol Cell. 2024 May 1;35(5):ed1. doi: 10.1091/mbc.E24-04-0148.NO ABSTRACTPMID:38691466 | DOI:10.1091/mbc.E24-04-0148 (Source: Mol Biol Cell)
Source: Mol Biol Cell - May 1, 2024 Category: Molecular Biology Authors: Matthew D Welch Source Type: research
Molecular Testing in Lung Cancer: Recommendations and Update
Surg Pathol Clin. 2024 Jun;17(2):307-320. doi: 10.1016/j.path.2023.11.012. Epub 2023 Dec 16.ABSTRACTAdoption of molecular testing in lung cancer is increasing. Molecular testing for staging and prediction of response for targeted therapy remain the main indications, and although utilization of blood-based testing for tumor is growing, the use of the diagnostic cytology and tissue specimens is equally important. The pathologist needs to optimize reflex testing, incorporate stage-based algorithms, and understand types of tests for timely and complete assessment in the majority of cases. When tissue is limited, testing should...
Source: Clinical Lung Cancer - May 1, 2024 Category: Cancer & Oncology Authors: Alain C Borczuk Source Type: research
Functional implications of NHR-210 enrichment in C. elegans cephalic sheath glia: insights into metabolic and mitochondrial disruptions in Parkinson's disease models
In conclusion, the deficiency of the nuclear hormone receptor nhr-210 in alpha-synuclein expressing strain of C. elegans, results in altered mitochondrial function, coupled with alterations in vital metabolite levels. These findings underline the functional and physiological importance of nhr-210 enrichment in CEPsh glia.PMID:38691171 | DOI:10.1007/s00018-024-05179-2 (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - May 1, 2024 Category: Cytology Authors: Rohil Hameed Anam Naseer Ankit Saxena Mahmood Akbar Pranoy Toppo Arunabh Sarkar Sanjeev K Shukla Aamir Nazir Source Type: research
Update in genetic and epigenetic causes of hypertension
Cell Mol Life Sci. 2024 Apr 30;81(1):201. doi: 10.1007/s00018-024-05220-4.ABSTRACTHypertension is a heritable disease that affects one-fourth of the population and accounts for about 50% of cardiovascular deaths. The genetic basis of hypertension is multifaceted, involving both monogenic and most commonly complex polygenic forms. With the advent of the human genome project, genome-wide association studies (GWAS) have identified a plethora of loci linked to hypertension by examining common genetic variations. It's notable, however, that the majority of these genetic variants do not affect the protein-coding sequences, posin...
Source: Cellular and Molecular Life Sciences : CMLS - May 1, 2024 Category: Cytology Authors: Arya Mani Source Type: research
Pyroptosis inhibitors MCC950 and VX-765 mitigate myocardial injury by alleviating oxidative stress, inflammation, and apoptosis in acute myocardial hypoxia
Exp Cell Res. 2024 Apr 29:114061. doi: 10.1016/j.yexcr.2024.114061. Online ahead of print.ABSTRACTAcute myocardial infarction (AMI) is a prevalent cardiovascular disease with high morbidity and mortality rates worldwide. Pyroptosis is an inflammatory form of programmed cell death that has been linked to various pathological conditions. However, its exact contribution to the onset and progression of heart injury in AMI has not yet fully elucidated. Herein, we established mouse AMI model by ligating the left anterior descending artery and performed transcriptome analysis during the early phase of AMI. Mouse HL-1 and human AC...
Source: Experimental Cell Research - May 1, 2024 Category: Cytology Authors: Xing Ye Zi-Jie Lin Guang-Hui Hong Zhi-Min Wang Run-Ting Dou Jun-Yi Lin Jian-Hui Xie Yi-Wen Shen Source Type: research
A cellular tilting mechanism important for dynamic tissue shape changes and cell differentiation in Drosophila
Dev Cell. 2024 Apr 29:S1534-5807(24)00236-3. doi: 10.1016/j.devcel.2024.04.011. Online ahead of print.ABSTRACTDynamic changes in three-dimensional cell shape are important for tissue form and function. In the developing Drosophila eye, photoreceptor differentiation requires the progression across the tissue of an epithelial fold known as the morphogenetic furrow. Morphogenetic furrow progression involves apical cell constriction and movement of apical cell edges. Here, we show that cells progressing through the morphogenetic furrow move their basal edges in opposite direction to their apical edges, resulting in a cellular ...
Source: Developmental Cell - May 1, 2024 Category: Cytology Authors: Liyuan Sui Christian Dahmann Source Type: research
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
Am J Physiol Cell Physiol. 2024 Mar 4. doi: 10.1152/ajpcell.00654.2023. Online ahead of print.ABSTRACTSkeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in...
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Simon I Dreher Paul Grubba Christine von Toerne Alessia Moruzzi Jennifer Maurer Thomas Goj Andreas L Birkenfeld Andreas Peter Peter Loskill Stefanie M Hauck Cora Weigert Source Type: research
Brachyury Promotes Extracellular Matrix Synthesis through Transcriptional Regulation of Smad3 in Nucleus Pulposus
In conclusion, this study reveals that Bry positively regulates the synthesis of collagen II and aggrecan in NP through transcriptional activation of Smad3.PMID:38690917 | DOI:10.1152/ajpcell.00475.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Yanzhang Xia Yinghui Wu Yuhao Gong Caichun Yue Linfeng Tao Tianwen Xin Cong Shen Yue Zhu Minghong Shen Jun Shen Source Type: research
CD200R promotes high glucose-induced oxidative stress and damage in human retinal pigment epithelial cells by activating the mTOR signaling pathway
This study establishes the crucial role of CD200R in HG-induced oxidative stress and identifies potential therapeutic avenues for the treatment of DR.PMID:38692160 | DOI:10.1016/j.tice.2024.102381 (Source: Tissue and Cell)
Source: Tissue and Cell - May 1, 2024 Category: Cytology Authors: Yaguang Hu Ting Wei Shan Gao Ning Gao Li Chen Qiaochu Cheng Source Type: research
The relation between autophagy modulation by intermittent fasting and aquaporin 2 expression in experimentally induced diabetic nephropathy in albino rat
This study aimed to describe the relation between autophagy modulation via intermittent fasting (IF) and renal AQP2 expression and polyuria in case of DN. We divided the rats into control, DN and IF groups. After 2 and 4 weeks of diabetes induction, blood glucose (BG), serum creatinine (Scr), urine volume, and 24 hours urine protein (UP) were examined. Diabetic nephropathy histopathological index (DNHI) was calculated to evaluate histopathological changes. Immunohistochemistry and real-time PCR were performed to measure the levels of AQP2 and the autophagy marker; LC3 in kidney tissue. DNHI was correlated to the PCR and im...
Source: Tissue and Cell - May 1, 2024 Category: Cytology Authors: Nora Hisham Hassan Dalia Saleh Salwa M Abo El-Khair Shaima M Almasry Amira Ibrahim Source Type: research
Brachyury Promotes Extracellular Matrix Synthesis through Transcriptional Regulation of Smad3 in Nucleus Pulposus
In conclusion, this study reveals that Bry positively regulates the synthesis of collagen II and aggrecan in NP through transcriptional activation of Smad3.PMID:38690917 | DOI:10.1152/ajpcell.00475.2023 (Source: American Journal of Physiology. Cell Physiology)
Source: American Journal of Physiology. Cell Physiology - May 1, 2024 Category: Cytology Authors: Yanzhang Xia Yinghui Wu Yuhao Gong Caichun Yue Linfeng Tao Tianwen Xin Cong Shen Yue Zhu Minghong Shen Jun Shen Source Type: research
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
Am J Physiol Cell Physiol. 2024 Mar 4. doi: 10.1152/ajpcell.00654.2023. Online ahead of print.ABSTRACTSkeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in...
Source: American Journal of Physiology. Cell Physiology - May 1, 2024 Category: Cytology Authors: Simon I Dreher Paul Grubba Christine von Toerne Alessia Moruzzi Jennifer Maurer Thomas Goj Andreas L Birkenfeld Andreas Peter Peter Loskill Stefanie M Hauck Cora Weigert Source Type: research
NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2
CONCLUSIONS: This study provides new molecular insights into the role of NANOG as a key determinant of pluripotency in TGCTs through the regulation of MIR9-2-5p, a novel epigenetic modulator of cancer stemness. Our data also highlight the potential negative feedback mediated by MIR9-2-5p on NANOG expression, which could be exploited as a therapeutic strategy for the treatment of TGCTs.PMID:38693576 | DOI:10.1186/s13287-024-03724-1 (Source: Cell Research)
Source: Cell Research - May 1, 2024 Category: Cytology Authors: Ryan P Cardenas Ahmad Zyoud Alan McIntyre Ramiro Alberio Nigel P Mongan Cinzia Allegrucci Source Type: research
