Characterization of cells expressing lipocalin-2 (LCN2) as a reporter
Biochem Cell Biol. 2024 May 2. doi: 10.1139/bcb-2024-0013. Online ahead of print.ABSTRACTLipocalin-2 (LCN2), an effector molecule of the innate immune system that is small enough to be tagged as a reporter molecule, can be coupled with the ferric ion through a siderophore such as enterobactin (Ent). Mintbody (modification-specific intracellular antibody) can track a posttranslational protein modification in epigenetics. We constructed plasmids expressing the LCN2 hybrid of mintbody to examine the potential of LCN2 as a novel reporter for magnetic resonance imaging (MRI). Cells expressing the LCN2 hybrid of mintbody showed ...
Source: Biochemistry and Cell Biology - May 2, 2024 Category: Biochemistry Authors: Kimihiko Sugaya Source Type: research
Absence of ATM leads to altered NK cell function in mice
Clin Immunol. 2024 Apr 30:110233. doi: 10.1016/j.clim.2024.110233. Online ahead of print.ABSTRACTAtaxia-telangiectasia (A-T) is a rare disorder caused by genetic defects of A-T mutated (ATM) kinase, a key regulator of stress response, and characterized by neurodegeneration, immunodeficiency, and high incidence of cancer. Here we investigated NK cells in a mouse model of A-T (Atm-/-) showing that they are strongly impaired at killing tumor cells due to a block of early signaling events. On the other hand, in Atm-/- littermates with thymic lymphoma NK cell cytotoxicity is enhanced as compared with ATM-proficient mice, possib...
Source: Clinical Immunology - May 2, 2024 Category: Allergy & Immunology Authors: Daniela Angela Covino Maria Giovanna Desimio Alessandro Giovinazzo Bruna Sabino Pinho de Oliveira Matilde Merolle Daniela Marazziti Manuela Pellegrini Margherita Doria Source Type: research
Assessing the impact of lubricant on liquid-based Pap smear test outcomes: a randomized clinical trial
CONCLUSION: The use of lubricant gel in patients can reduce pain in patients during examination and testing, but does not affect the cytological and pathological results of patients.PMID:38694378 | PMC:PMC11060314 | DOI:10.1097/MS9.0000000000001803 (Source: Annals of Medicine)
Source: Annals of Medicine - May 2, 2024 Category: Internal Medicine Authors: Mansooreh Yaraghi Sarvenaz Gharavi Ayda Najafian Vajihe Hazari Source Type: research
Comprehensive analysis of recently discovered lncRNA-associated competing endogenous RNA network in nasopharyngeal carcinoma
Pathol Res Pract. 2024 Apr 16;258:155314. doi: 10.1016/j.prp.2024.155314. Online ahead of print.ABSTRACTNasopharyngeal carcinoma (NPC) arises from the epithelium of the nasopharynx and is characterized by geography-dependent incidence. Despite the high mortality rate, specifically in some ethnic groups, the mechanisms underlying NPC pathogenesis are not thoroughly understood and there is an urgent need to detect the potential and clinically applicable biomarkers to ameliorate the overall survival rate and improve the prognosis of patients. In recent years, research has increasingly focused on the importance of long non-cod...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Guochen Sun Feng Liu Maryam Lesany Sara Nemati Source Type: research
Expression analysis of necroptosis related genes and lncRNAs in patients with pituitary neuroendocrine tumors
Pathol Res Pract. 2024 Apr 30;258:155332. doi: 10.1016/j.prp.2024.155332. Online ahead of print.ABSTRACTNecroptosis can either be the cause of tumorigenesis or it can impede its process. Recently, it has been proved that long non-coding RNAs (lncRNAs) have different crucial roles at cellular level, especially on cell death. Regarding the important role of necroptosis and lncRNAs in the pathogenesis of different cancers, especially pituitary adenomas (PAs), we assessed expression levels of two necroptosis related genes, namely TRADD and BIRC2, in addition to three related lncRNAs, namely FLVCR1-DT, MAGI2-AS3, and NEAT1 in P...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Arian Askari Mohammad Reza Darabi Solat Eslami Elena Jamali Guive Sharifi Soudeh Ghafouri-Fard Nader Akbari Dilmaghani Source Type: research
α,β-trehalose, an intracellular substance in resting cyst of colpodid ciliates as a key to environmental tolerances
In this study, we identified α,β-trehalose in the resting cyst, a dormancy cell form characterized by extreme tolerance to environmental stresses, of the ciliated protist Colpoda cucullus, using high-performance liquid chromatography (HPLC), and a proton nuclear magnetic resonance (1H NMR). Gene expression analysis revealed that the expression of trehalose-6-phosphate synthase (TPS), glycosyltransferase (GT), alpha-amylase (AMY), and trehalose transporter 1 (TRET1), were up-regulated in encystment, while the expression of α-glucosidase 2 (AG2) and trehalase (TREH) was up-regulated in excystment. These results suggest th...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Yoichiro Sogame Makoto Ogata Shuntaro Hakozaki Yuta Saito Tomohiro Suzuki Ryota Saito Futoshi Suizu Kozo Watanabe Source Type: research
Features of the DNA Escherichia coli RecN interaction revealed by fluorescence microscopy and single-molecule methods
Biochem Biophys Res Commun. 2024 Apr 25;716:150009. doi: 10.1016/j.bbrc.2024.150009. Online ahead of print.ABSTRACTThe SOS response is a condition that occurs in bacterial cells after DNA damage. In this state, the bacterium is able to reсover the integrity of its genome. Due to the increased level of mutagenesis in cells during the repair of DNA double-strand breaks, the SOS response is also an important mechanism for bacterial adaptation to the antibiotics. One of the key proteins of the SOS response is the SMC-like protein RecN, which helps the RecA recombinase to find a homologous DNA template for repair. In this work...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Viktoria D Roshektaeva Aleksandr A Alekseev Alexey D Vedyaykin Viktor A Vinnik Dmitrii M Baitin Irina V Bakhlanova Georgii E Pobegalov Mikhail A Khodorkovskii Natalia E Morozova Source Type: research
Quality of care measurement for patients with ovarian cancer in Japan
CONCLUSIONS: The quality of ovarian cancer care should be continuously assessed to encourage the use of best practices. These indicators may be a useful tool for this purpose.PMID:38697202 | DOI:10.1111/jog.15961 (Source: Cell Research)
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Tamaki Kakuwa Tomone Watanabe Mariko Niino Akira Kawata Toyomi Satoh Noriomi Matsumura Yoshihito Yokoyama Kei Kawana Yasuyuki Hirashima Satoru Kyo Masanori Yasuda Kenichi Harano Hiroko Machida Hideki Tokunaga Masanori Kaneuchi Tsutomu Tabata Yoichi Kobaya Source Type: research
Raman Spectroscopy Analysis of Human Amniotic Fluid Cells from fetuses with Myelomeningocele
Exp Cell Res. 2024 Apr 30:114048. doi: 10.1016/j.yexcr.2024.114048. Online ahead of print.ABSTRACTPrenatal surgery for the treatment of spina bifida (myelomeningocele, MMC) significantly enhances the neurological prognosis of the patient. To ensure better protection of the spinal cord by large defects, the application of skin grafts produced with cells gained from the amniotic fluid is presently studied. In order to determine the most appropriate cells for this purpose, we tried to shed light on the extremely complex amniotic fluid cellular composition in healthy and MMC pregnancies. We exploited the potential of micro-Ram...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Luca Pontiggia Katarzyna Michalak-Micka Nadine H ürlimann Hesham K Yosef Roland B öni Agnes S Klar Martin Ehrbar Nicole Ochsenbein-K ölble Thomas Biedermann Ueli Moehrlen Source Type: research
Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission
This study investigates dulaglutide's effects on peripheral blood levels of CD34+ and CD133+ cells in a mouse model of lower limb ischemia and its protective mechanisms against high-glucose-induced damage in endothelial cells. Results demonstrated that dulaglutide significantly improves blood flow, reduces tissue damage and inflammation in ischemic limbs, and enhances glycemic control. Furthermore, dulaglutide alleviated high-glucose-induced endothelial cell damage, evident from improved tube formation, reduced reactive oxygen species accumulation, and restored endothelial junction integrity. Mechanistically, dulaglutide m...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Xi Mei Yao Li Jinlin Wu Lumiu Liao Di Lu Ping Qiu Hui-Lan Yang Ming-Wei Tang Xin-Ying Liang Dongfang Liu Source Type: research
Cyclic strain alters the transcriptional and migratory response of scleral fibroblasts to TGF β
Exp Eye Res. 2024 Apr 30:109917. doi: 10.1016/j.exer.2024.109917. Online ahead of print.ABSTRACTIn glaucoma, scleral fibroblasts are exposed to IOP-associated mechanical strain and elevated TGFβ levels. These stimuli, in turn, lead to scleral remodeling. Here, we examine the scleral fibroblast migratory and transcriptional response to these stimuli to better understand mechanisms of glaucomatous scleral remodeling. Human peripapillary scleral (PPS) fibroblasts were cultured on parallel grooves, treated with TGFβ (2 ng/ml) in the presence of vehicle or TGFβ signaling inhibitors, and exposed to uniaxial strain (1 Hz, 5%, ...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Ann Mozzer Ian Pitha Source Type: research
The alteration of NK cells phenotypes related to the functions and dengue disease outcomes
This study explored the profile of NK cells in dengue infected pediatric patients with different degrees of disease severity. DHF patients contained higher frequency of activated NK cells but lower ratio of CD56dim:CD56bright NK subsets. Activated NK cells exhibited alterations in several NK receptors. Interestingly, the frequencies of NKp30 expressing activated NK cells were more pronounced in dengue fever (DF) than in DHF pediatric patients. In vitro functional analysis indicated that degranulation of NK cells in responding to dengue infected dendritic cells (DCs) required cell-cell contact and type I IFNs. Meanwhile, In...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Napas Taechasan Iris Scherwitzl Piyada Supasa Wanwisa Dejnirattisai Kanokwan Sriruksa Wannee Limpitikul Prida Malasit Gavin R Screaton Juthathip Mongkolsapaya Thaneeya Duangchinda Source Type: research
GABA transporter mGat4 is involved in multiple neural functions in mice
Biochim Biophys Acta Mol Cell Res. 2024 Apr 30:119740. doi: 10.1016/j.bbamcr.2024.119740. Online ahead of print.ABSTRACTγ-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. The termination of GABA transmission is through the action of GABA transporters (GATs). mGAT4 (encoded by Slc6a11) is another GAT besides GAT1 (encoded by Slc6a1) that functions in GABA reuptake in CNS. Research on the function of mGAT4 is still in its infancy. We developed an mGat4 knockout mouse model (mGat4-/- mice) and performed a series of behavioral analyses for the first time to study the effect of m...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Yue Ying Weitong Liu Haoyue Wang Jiahao Shi Zhugang Wang Jian Fei Source Type: research
Inhibition of δ-catenin palmitoylation slows the progression of prostate cancer
In this study, we utilized prostate cancer cells overexpressing mutant δ-catenin (J6A cells) to induce a depalmitoylation phenotype and investigate its effect on prostate cancer. Our results indicated that depalmitoylation of δ-catenin not only reduced its membrane expression but also promoted its degradation in the cytoplasm, resulting in a decrease in the effect of EGFR and E-cadherin signaling. Consequently, depalmitoylation of δ-catenin reduced the proliferation and metastasis of prostate cancer cells. Our findings provide novel insights into potential therapeutic strategies for controlling the progression of prosta...
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Beini Wang Rui Zhou Jin Wu Hangun Kim Kwonseop Kim Source Type: research
Tanshinone IIA alleviates pulmonary fibrosis by modulating glutamine metabolic reprogramming based on [U- < sup > 13 < /sup > C < sub > 5 < /sub > ]-glutamine metabolic flux analysis
CONCLUSION: Tan IIA reverses glutamine metabolic reprogramming, reduces mitochondrial energy expenditure, and inhibits collagen matrix synthesis by modulating potential targets in glutamine metabolism. This novel perspective sheds light on the essential role of glutamine metabolic reprogramming in PF.PMID:38697470 | DOI:10.1016/j.jare.2024.04.029 (Source: Cell Research)
Source: Cell Research - May 2, 2024 Category: Cytology Authors: Baixi Shan Congying Guo Haoyan Zhou Jun Chen Source Type: research
