Mesoscale Metabolic Channeling Revealed by Multimodal Microscopy
Res Sq [Preprint]. 2024 Apr 17:rs.3.rs-4096781. doi: 10.21203/rs.3.rs-4096781/v1.ABSTRACTMetabolic homeostasis within cells and tissues requires engagement of catabolic and anabolic pathways consuming nutrients needed to generate energy to drive these and other subcellular processes. However, the current understanding of cell homeostasis and metabolism, including how cells utilize nutrients, comes largely from tissue and cell models analyzed after fractionation. These bulk strategies do not reveal the spatial characteristics of cell metabolism at the single cell level, and how these aspects relate to the location of cells ...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Rafael Arrojo E Drigo Aliyah Habashy Christopher Acree Keun-Young Kim Thomas Deerinck Emilee Patterson Louise Lantier Owen McGuinness Mark Ellisman Source Type: research
Voriconazole Metabolism is Associated with the Number of Skin Cancers Per Patient
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4152279. doi: 10.21203/rs.3.rs-4152279/v1.ABSTRACTVoriconazole exposure is associated with skin cancer, but it is unknown how the full spectrum of its metabolizer phenotypes impacts this association. We conducted a retrospective cohort study to determine how variation in metabolism of voriconazole as measured by metabolizer status of CYP2C19 is associated with the total number of skin cancers a patient develops and the rate of development of the first skin cancer after treatment. There were 1,739 organ transplant recipients with data on CYP2C19 phenotype. Of these, 134 were exposed to...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Jacqueline I Ike Isabelle T Smith Dominique Mosley Christopher Madden Sarah Grossarth Briana R Halle Adam Lewis Frank Mentch Hakon Hakonarson Lisa Bastarache Lee Wheless Source Type: research
Supinoxin blocks Small Cell Lung Cancer Progression by Inhibiting Mitochondrial Respiration through the RNA Helicase DDX5
Res Sq [Preprint]. 2024 Apr 15:rs.3.rs-4169007. doi: 10.21203/rs.3.rs-4169007/v1.ABSTRACTDDX5 is a DEAD-box RNA helicase that is overexpressed and implicated in progression of several cancers 1-4. One of these is small cell lung cancer (SCLC). Our laboratory has demonstrated that the RNA helicase DDX5 is essential for the invasive growth of SCLC and mitochondrial respiration 5. SCLC is an extremely lethal, recalcitrant tumor 6,7, causing 250,000 deaths annually worldwide 8 and currently lacking effective treatments 9,10. Supinoxin (RX 5902), a compound having anti-cancer activity 11, is a known target of phosphor-DDX5 12,1...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Elizabeth Tran Subhadeep Das Matthew Russon Maria Zea Zheng Xing Sandra Torregrosa-Allen Heidi Cervantes Bennett Elzey Haley Harper Source Type: research
Single-molecule RNA-FISH analysis reveals stochasticity in reactivation of latent HIV-1 regulated by Nuclear Orphan Receptors NR4A and cMYC
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4166090. doi: 10.21203/rs.3.rs-4166090/v1.ABSTRACTHIV-1 eradication strategies require complete reactivation of HIV-1 latent cells by Latency Reversing Agents (LRA). Current methods lack effectiveness due to incomplete proviral reactivation. We employed a single-molecule RNA-FISH (smRNA-FISH) and FISH-Quant analysis and found that proviral reactivation is highly variable from cell-to-cell, stochastic, and occurs in bursts and waves, with different kinetics in response to diverse LRAs. Approximately 1-5% of latent cells exhibited stochastic reactivation without LRAs. Through single-cel...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Annalena LaPorte Rajiv Pathak Carolina Eliscovich Laura Martins Rachel Nell Adam Spivak Masako Suzuki Vicente Planelles Robert Singer Ganjam Kalpana Source Type: research
The Prognosis for Delayed Immune Recovery in HIV-Infected Children might be Associated with Pre-cART CD4 + T cell Count Irrespective of Co-Infection with Tuberculosis
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4243586. doi: 10.21203/rs.3.rs-4243586/v1.ABSTRACTBackground : Immune reconstitution following the initiation of combination antiretroviral therapy (cART) significantly impacts the prognosis of individuals infected with human immunodeficiency virus (HIV). Our previous studies have indicated that the baseline CD4 + T cells count and percentage before cART initiation are predictors of immune recovery in TB-negative children infected with HIV, with TB co-infection potentially causing a delay in immune recovery. However, it remains unclear whether these predictors consistently impact immu...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Funsho Ogunshola Ruhul Khan Musie Ghebremichael Source Type: research
Homeostasis of cytoplasmic crowding by cell wall fluidization and ribosomal counterions
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4138690. doi: 10.21203/rs.3.rs-4138690/v1.ABSTRACTIn bacteria, algae, fungi, and plant cells, the wall must expand in concert with cytoplasmic biomass production, otherwise cells would experience toxic molecular crowding 1,2 or lyse. But how cells achieve expansion of this complex biomaterial in coordination with biosynthesis of macromolecules in the cytoplasm remains unexplained 3 , although recent works have revealed that these processes are indeed coupled 4,5 . Here, we report a striking increase of turgor pressure with growth rate in E. coli , suggesting that the speed of cell wal...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Markus Basan Avik Mukherjee Yanqing Huang Seungeun Oh Carlos Sanchez Yu-Fang Chang Xili Liu Gary Bradshaw Nina Benites Johan Paulsson Marc Kirschner Yongjin Sung Jens Elgeti Source Type: research
Nirmatrelvir/ritonavir treatment of patients with COVID-19 taking tacrolimus: case series describing the results of drug-drug interactions
We describe the cases of two patients treated with nirmatrelvir/ritonavir: a patient who had undergone kidney transplantation and another with a history of hematopoietic stem cell transplantation. Toxic concentrations of tacrolimus were induced in both. This case series highlights the risk associated with the concomitant administration of tacrolimus and nirmatrelvir/ritonavir.PMID:38698526 | DOI:10.1177/03000605241247705 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Dan Shen Yinhua Gong Yulan Qian Jianguo Zhu Jie Gao Source Type: research
Umbilical Cord Blood and UC-MSCs Combined with Low-Dose Immunosuppressant in the Treatment of Elderly Patients with Pure Red Cell Aplastic: A Case Series
CONCLUSION: This report provides an effective regimen of using UCB and UC-MSCs combined with low-dose CsA (1-3 mg/kg/d) to treat PRCA, especially for elderly patients with multiple complications who cannot use the conventional dosage.PMID:38698745 | DOI:10.2174/011574888X290378240424075002 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Wei-Wei Zhu Sujing Zhuang Zhe Yu Xin Li Tian-Jie Han Yue Ma Li-Jun Li Zhi-Rui Zhao Source Type: research
Single-Cell Proteomics Accelerates toward Proteoforms
J Proteome Res. 2024 May 3;23(5):1545-1546. doi: 10.1021/acs.jproteome.4c00290.NO ABSTRACTPMID:38698756 | DOI:10.1021/acs.jproteome.4c00290 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Sarah N Sipe Nikolai Slavov Source Type: research
Forward programming human pluripotent stem cells into microglia
Trends Cell Biol. 2024 May 3:S0962-8924(24)00069-2. doi: 10.1016/j.tcb.2024.03.006. Online ahead of print.ABSTRACTMicroglia play vital roles in embryonic and post-natal development, homeostasis, and pathogen defence in the central nervous system. Human induced pluripotent stem cell (hiPSC)-based methods have emerged as an important source for the study of human microglia in vitro. Classical approaches to differentiate hiPSCs into microglia suffer from limitations including extended culture periods, consistency, and efficiency. More recently, forward programming has arisen as a promising alternative for the manufacture of b...
Source: Trends in Cell Biology - May 3, 2024 Category: Cytology Authors: J úlia Csatári Heinz Wiendl Matthias Pawlowski Source Type: research
Tubulin posttranslational modifications through the lens of new technologies
Curr Opin Cell Biol. 2024 May 2;88:102362. doi: 10.1016/j.ceb.2024.102362. Online ahead of print.ABSTRACTThe Tubulin Code revolutionizes our understanding of microtubule dynamics and functions, proposing a nuanced system governed by tubulin isotypes, posttranslational modifications (PTMs) and microtubule-associated proteins (MAPs). Tubulin isotypes, diverse across species, contribute structural complexity, and are thought to influence microtubule functions. PTMs encode dynamic information on microtubules, which are read by several microtubule interacting proteins and impact on cellular processes. Here we discuss recent tec...
Source: Current Opinion in Cell Biology - May 3, 2024 Category: Cytology Authors: Gonzalo Alvarez Viar Gaia Pigino Source Type: research
SIRT1 maintains bone homeostasis by regulating osteoblast glycolysis through GOT1
In conclusion, our study suggested that Sirt1 played a crucial role in regulating osteoblast metabolism to maintain bone homeostasis through its deacetylase activity on GOT1. These findings provided a novel insight into the potential targeting of osteoblast metabolism for the treatment of bone-related diseases.PMID:38700532 | DOI:10.1007/s00018-023-05043-9 (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - May 3, 2024 Category: Cytology Authors: Xinxin Jin Xulei Sun Xiao Ma Zixuan Qin Xin Gao Xiaomin Kang Huixia Li Hongzhi Sun Source Type: research
Sputum Neutrophil Gelatinase-Associated Lipocalin as a Biomarker in Asthma-COPD Overlap
CONCLUSION: The present study investigated the sputum NGAL levels as a biomarker in ACO identified by the syndromic approach. Sputum NGAL, a biomarker associated with airway inflammation in airway diseases, was supportive of clinically differentiating ACO from asthma to COPD. How to cite this article: Babu A, Narayanswamy H, Baburao A. Sputum Neutrophil Gelatinase-Associated Lipocalin as a Biomarker in Asthma-COPD Overlap. J Assoc Physicians India 2023;71(9):34-38.PMID:38700299 | DOI:10.59556/japi.71.0320 (Source: Journal of the Association of Physicians of India)
Source: Journal of the Association of Physicians of India - May 3, 2024 Category: General Medicine Authors: Ajay Babu Huliraj Narayanswamy Archana Baburao Source Type: research
The efficacy and safety of continuous intravenous infusion of rh-endostatin combined with platinum-based doublet chemotherapy for advanced non-small-cell lung cancer
CONCLUSIONS: The incorporation of a 5-day continuous intravenous infusion of rh-endostatin into platinum-based doublet chemotherapy has demonstrated both safety and efficacy in the treatment of advanced NSCLC. Furthermore, the baseline serum levels of CEA may potentially function as a predictor for the efficacy of rh-endostatin when combined with chemotherapy in NSCLC patients.CLINICALTRIALS: GOV: NCT05574998.PMID:38700579 | DOI:10.1007/s10637-024-01439-x (Source: Cancer Control)
Source: Cancer Control - May 3, 2024 Category: Cancer & Oncology Authors: Xinyi Liu Zihan Guo Lin Su Anli Zuo Min Gao Xiang Ji Jiameng Lu Shuran Yang Yunxiu Jiang Degan Lu Source Type: research
High-Risk Human Papilloma Virus Genotype Distribution and Correlation with Cervical Cytomorphological Data in Turkish and Immigrant Women in Mersin Province
New Microbiol. 2024 May;47(1):88-97.ABSTRACTHuman papilloma virus (HPV) is the most common sexually transmitted viral agent in the world and the most common cause of cervical cancer. HPV prevalence and genotype distribution vary by region and demographic data. In a province in the south of Turkey that constantly receives immigration, we aimed to determine the prevalence of high-risk HPV (HR-HPV) genotypes, evaluate the compatibility between cervical Pap smear cytology results patients and HR-HPVs, and make an up-to-date contribution to the elucidation of epidemiological data. In this single-centre study, a total of 12,641 ...
Source: New Microbiologica - May 3, 2024 Category: Microbiology Authors: Murat Yaman Hacer Ece Arslan Özcan Ayfer Bakir Source Type: research
