Blood stream infections during the first wave of COVID-19. A short microbiological retrospective picture at Papa Giovanni XXIII Hospital, Bergamo, Italy
New Microbiol. 2021 Jan;44(1):51-58.ABSTRACTThis paper aims to describe the etiology of bloodstream infections in COVID-19, Papa Giovanni XXIII Hospital, Bergamo, Italy. Two periods were evaluated: February 22-May 21, 2019/2020. We considered: the number of patients and blood culture sets, species of isolates (bacteria, specifically those indicated by EARS criteria; CoNS; Candida albicans) and their antibiotic sensitivity. In 2020 Escherichia coli and Carbapenemase-producing Klebsiella pneumoniae disappeared. Candida albicans and MDR Pseudomonas aeruginosa, Enterococcus faecium and Acinetobacter baumannii were largely pres...
Source: New Microbiologica - March 23, 2021 Category: Microbiology Authors: Marina Cuntr ò Alberto Manisco Davide Guarneri Gianluca Zuglian Francesca Vailati Marco Passera Marco Cavallini Annibale Raglio Claudio Farina Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Jan;44(1):59-61. Epub 2021 Feb 14.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:33582...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan;44(1):24-32. Epub 2021 Jan 14.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy control...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research

Importance of broth-enrichment culture in equine endometritis diagnosis
The objective of this study was to investigate the diagnostic accuracy of the standard microbiological protocol to assure the evaluation of bacterial endometritis in the equine clinical practice. Four hundred fifty-two equine uterine swabs were seeded on different types of agar plates and then in a broth-enrichment (Brain Heart Infusion Broth) before plating by using the same media the day after. The prevalence of positivity was 33.7% following direct plating and 66.3% following use of added enrichment-broth phase before seeding on solid media. Furthermore, the prevalence of isolated bacteria included E. coli (29.7%) and S...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Francesca Paola Nocera Monica Ambrosio Antonella Conte Tommaso Di Palma Sibilla Castaldo Maria Pia Pasolini Filomena Fiorito Luisa De Martino Source Type: research

Cervical lymph node tuberculosis and TNF, IL8, IL10, IL12B and IFNG polymorphisms
New Microbiol. 2021 Jan 14;44(1). Online ahead of print.ABSTRACTCervical lymph node tuberculosis (LNTB) is the most common manifestation of extrapulmonary tuberculosis, resulting from the interaction of environmental and genetic factors. The immune response against TB is regulated by several cytokines, which have single nucleotide polymorphisms (SNPs), leading to different levels of expression. The aim of this study was to evaluate the association of LNTB with the TNF, IL8, IL10, IL12B and IFNG gene polymorphisms in Mexican patients. We investigated the association of ten SNPs in 14 patients with LNTB and 138 healthy contr...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Olga Plowes-Hern ández H éctor Prado-Calleros Sara Arroyo-Escalante Beatriz Zavaleta-Villa Javier Flores-Osorio Aurora Ibarra Arce Mirza Romero-Valdovinos Ang élica Olivo-Díaz Source Type: research

Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.PMID:33582822 (Source: New Microbiologica)
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Thitinan Kitisin Watcharamat Muangkaew Sumate Ampawong Palatip Chutoam Natthapaninee Thanomsridetchai Marut Tangwattanachuleeporn Passanesh Sukphopetch Source Type: research

Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study)
New Microbiol. 2021 Feb 14;44(1). Online ahead of print.ABSTRACTIn the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.PMID:335...
Source: New Microbiologica - February 14, 2021 Category: Microbiology Authors: Sandrine Castelain Catherine Fran çois Baptiste Demey Aurelien Aubry Jean-Philippe Lanoix Gilles Duverlie Jean-Luc Schmit Etienne Brochot Source Type: research