The effect of culture media dilution on recovery of rapidly growing mycobacteria.
Authors: Li J, Wang J, Sun H, Huo F, Shang Y, Li S Abstract Nontuberculous mycobacteria (NTM) are important environmental opportunistic pathogens of human and animals. Rapidly growing mycobacteria (RGM) are important emerging pathogens causing NTM infections. Unfortunately, the majority of microorganisms in the environment resist cultivation in the laboratory. The aim of the study was to investigate whether the nutrients in the medium have impact on the growth of RGM in vitro. We first assessed the growth rate of rapidly growing mycobacteria strains in broth medium with different dilutions, including M. abscessus, ...
Source: New Microbiologica - December 8, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Flash on gut microbiome in gestational diabetes: a pilot study.
Authors: Festa C, Drago L, Martorelli M, Di Marino VP, Bitterman O, Corleto CC, Corleto VD, Napoli A Abstract Pregnancy induces a deep modification of women's gut microbiota composition. These changes may influence hormonal and metabolic factors, increasing insulin resistance and leading to hyperglycaemia in susceptible women. Data on 29 women in pregnancy showed insignificant reductions in the Bacteroidetes/Firmicutes ratio in women with (n.14) and without (n.15) gestational diabetes (GDM). Gut microbiota compositions at the genera and species level were further analysed in ten pregnant women with and ten without ...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Nanosilver gel as an endodontic alternative against Enterococcus faecalis in an in vitro root canal system in Mexican dental specimens.
The objective of this investigation is to evaluate the antibacterial effect of a gel preparation containing silver nanoparticles (Ag-NP) against E. faecalis present in the walls of the canal root. 60 extracted human uniradicular teeth that were instrumented with Wave One Gold (Denstplay / USA) and subsequently contaminated with Enterococcus faecalis. For antibacterial evaluation, intra-conduction medication was placed and several groups were formed: a) Ag-NP 300 ug / MI gel, b) Ag-NP 500 ug / MI gel, c) Ca (OH) 2 (Ultracal from Ultradent / USA) and the control group. They were incubated at 37°C and a sample was taken e...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Dentistry and Covid-19 pandemic: operative indications post-lockdown.
Authors: Enrico G, Elisabetta P, Giulia T, Paolo C Abstract A new Coronavirus, the seventh member of the Coronaviridae family, identified as SARS-CoV-2, spread in late December 2019 in the territory of Wuhan in China. CoV-2019 can be transmitted directly from person to person by respiratory drops, direct contact and contaminated material. Furthermore, 2019-nCov penetrates cells similarly to the SARS coronavirus, i.e., through the ACE2 receptor. This may promote human-to-human transmission. Patients and dental professionals are exposed daily to pathogenic microorganisms, including viruses and bacteria, which infect ...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

SARS-CoV-2 IgG/IgM Rapid Test as a Diagnostic Tool in Hospitalized Patients and Healthcare Workers, at a large Teaching Hospital in northern Italy, during the 2020 COVID-19 Pandemic.
We describe the outcome of a Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV-2) IgG/IgM rapid test, and discuss the potential suitability of antibody testing. Retrospective single cohort study on patients with suspected Coronavirus Disease 2019 (COVID-19) and asymptomatic Healthcare Workers, enrolled from March to April 2020. Subjects had quantitative PCR (qPCR) test for detection of SARS-CoV-2 via nasal swab and serological testing using the COVID-19 IgG/IgM Rapid Test (PRIMA Lab SA) immunochromatographic assay. Some subjects underwent chemiluminescence immunoassay (CLIA) after rapid test. The aim of the study wa...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Tracking over time the developing gut microbiota in newborns admitted to a neonatal intensive care unit during an outbreak caused by ESBL-producing Klebsiella pneumoniae.
Authors: Panelli S, Corbella M, Gazzola A, Piralla A, Girello A, Rampelli S, Candela M, Cambieri P Abstract The establishment of gut microbiota is reportedly aberrant in newborns admitted to neonatal intensive care units (NICUs), with detrimental long-term health impacts. Here, we vertically tracked the developing gut bacterial communities of newborns hosted in an NICU during an outbreak sustained by ESBL Klebsiella pneumoniae and compared colonized and noncolonized patients. Most communities were highly variable from one sampling point to the next, and dominated by few taxa, often Proteobacteria and Enterobacteria...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

There is a Role in Detection of SARS-CoV-2 in Conjunctiva and Tears: a comprehensive review.
Authors: Bernabei F, Versura P, Rossini G, Re MC Abstract Data on the involvement of the ocular surface and its relationship with Coronavirus disease 2019 (COVID-19) are still minimal and not univocal. The respiratory tract is the structure most affected by COVID-19, and the serious form of the disease is characterized by severe pneumonia, acute respiratory distress syndrome and hypercoagulation. However, accumulating evidence shows that other organs could be reached by the virus, thus causing further comorbidities. To date, the exact route / routes of transmission of COVID-19 are still unclear. The respiratory tra...
Source: New Microbiologica - November 4, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

The burden of Tuberculosis in a low-incidence territory: contribution of foreign population in the disease epidemiology.
Authors: Facciolà A, Visalli G, D'Andrea G, Di Pietro A Abstract Tuberculosis (TB) still represents one of the most important causes of death worldwide. In Italy, TB is a relatively rare disease. This research aimed to evaluate the TB cases reported in the provincial territory of Messina, Italy, in order to assess the contribution of the different groups of the local population. We conducted a review of existing epidemiological data evaluating the trend of all TB notifications reported from 2001 to 2019. For the collection of the data, all the notifications were evaluated by analyzing the local and national ...
Source: New Microbiologica - October 8, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Potential clinical use of azithromycin against gastroenteritis-causing pathogens other than Campylobacter.
Authors: Casanovas-Moreno-Torres I, Gutiérrez-Soto B, Modovan TD, Expósito-Ruiz M, Navarro-Marí JM, Gutiérrez-Fernández J Abstract The activity of azithromycin against enteritis-producing agents other than Campylobacter spp. was studied. The susceptibility to azithromycin, through gradient test, of 88 clinical isolates (51 Salmonella spp., 23 Aeromonas spp., 10 Shigella sonnei and 4 Yersinia enterocolitica) for one year was studied prospectively. The results were compared with the activity of ampicillin, trimethoprim-sulfamethoxazole and ciprofloxacin by microdilution. For azithro...
Source: New Microbiologica - October 8, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Utilization of an in vitro biofabricated 3D skin as a pathological model of cutaneous candidiasis.
This study provides an alternative pathological model of cutaneous candidiasis, which can physiologically represent a close-up event during C. albicans. Moreover, it is rapid, costeffective, and reproducible of the in vitro biofabricated 3D skin model, and may further highlight the importance of utilizing in vivo-like conditions to improve high-throughput screening for drug discovery against several antifungal drug resistant pathogens. PMID: 33021319 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - October 8, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

SARS-CoV-2 pandemic: implications in the management of patients with colorectal cancer.
Authors: Di Marzo F, Fiori E, Sartelli M, Cennamo R, Coccolini F, Catena F, Calabretto M, Riveros Cabral RJ, Lombardi M, Baiocchi GL, Cardi M, Cusi MG, Cardi M Abstract The SARS-CoV-2 pandemic has already reached 3,207,248 patients with more than 225,000 deaths all over the world. Colorectal cancer is the third most diagnosed cancer worldwide, and the healthcare system is struggling to manage daily activities for elective cancer surgery. This review integrates clinical, microbiological, architectural and surgical aspects to develop indications on strategies to manage colorectal cancer patients and ensure safety dur...
Source: New Microbiologica - October 8, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Intestinal tuberculosis versus Inflammatory Bowel Diseases: a never-ending challenge.
Authors: Castellotti P, Riccardi N, Ferrarese M, Canetti D, Fanti D, Forti E, Reda M, Alexiadis S, Bonoldi E, Saporiti M, Perno CF, Ruffo Codecasa L Abstract Inflammatory Bowel Diseases (IBD) and intestinal tuberculosis (ITB) frequently share similar clinical, radiological, endoscopic and histologic features. The misdiagnosis of IBD can lead to worsening of ITB course, eventually with dissemination of Mycobacterium tuberculosis (MTB) due to immunosuppressive treatment. We herein report a challenging diagnosis of ITB, progressed from localized to disseminated, in a pregnant woman previously misdiagnosed with Crohn' ...
Source: New Microbiologica - July 14, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Parechovirus infection causing sepsis-like illness in newborns: a NICU approach.
Authors: Ancora G, Faldella G, Chiereghin A, Marsico C, Nigro CS, Lazzarotto T, Sambri V, Brusa G, Capretti MG Abstract Human parechovirus (HpeV) is an important emerging infection in young infants, able to cause sepsis-like disease and meningoencephalitis, especially in newborns. Among the 19 identified genotypes, HPeV1, 3 and 6 are the most common types involved in human infections; HPeV3 is the type mainly responsible for neonatal infections and for infections involving the central nervous system. Signs and symptoms overlap with those of a bacterial infection and patients are usually treated with broad spectrum ...
Source: New Microbiologica - July 14, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Genital and extra-genital Chlamydia trachomatis and Neisseria gonorrhoeae infections in young women attending a Sexually Transmitted Infections (STI) clinic.
Authors: Foschi C, Zagarrigo M, Belletti M, Marangoni A, Re MC, Gaspari V Abstract Chlamydia trachomatis and Neisseria gonorrhoeae are the most common agents of bacterial sexually transmitted infections (STIs) worldwide. Here, we evaluated genital and extra-genital C. trachomatis and N. gonorrhoeae infection prevalence in a cohort of young women attending an STI Outpatients Clinic in Italy. From May 2019 to December 2019, 134 women aged 18-26 years were enrolled. A vaginal, a pharyngeal and a rectal swab for the molecular detection of C. trachomatis and N. gonorrhoeae were collected from each patient. Chlamydia-pos...
Source: New Microbiologica - July 14, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Comparison between a novel molecular tool and conventional methods for diagnostic classification of bacterial vaginosis: is integration of the two approaches necessary for a better evaluation?
Authors: Scapaticci M, Da Rin G, Bartolini A Abstract The etiological cause of bacterial vaginosis (BV) is the change of the vaginal ecosystem characterized by a decrease of lactobacilli and an increase of other germs, such as Gardnerella vaginalis and Atopobium vaginae. Molecular tools have revolutionized the diagnosis of these conditions. The aim of this paper was to compare results obtained from 158 vaginal swabs collected from women aged between 18 and 59 years old and subjected to microscopic evaluation (Nugent Score), culture and to the multiparametric molecular assay Vaginitis and Vaginosis Multiplex-Tandem ...
Source: New Microbiologica - July 14, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Molecular characterization of Mycobacterium abscessus subspecies isolated from patients attending an Italian Cystic Fibrosis Centre.
Authors: Teri A, Sottotetti S, Arghittu M, Girelli D, Biffi A, D'Accico M, Daccò V, Gambazza S, Pizzamiglio G, Trovato A, Tortoli E, Colombo C, Cariani L Abstract Mycobacterium abscessus (MABS) infection represents significant management challenge in cystic fibrosis (CF) patients. This retrospective study (2005-2016) aims to determine the prevalence of the subspecies of MABS isolated from CF patients, to evaluate the persistence over the years of a single subspecies of MABS and to correlate mutations responsible for macrolides and amikacin resistance with MIC values. We investigated 314 strains (1 isolate/pa...
Source: New Microbiologica - July 14, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Tetracycline resistance in Moraxella catarrhalis clinical strains isolated in Poland.
Authors: Król-Turmińska K, Olender A, Bogut A Abstract Moraxella catarrhalis is considered an important, exclusively human respiratory tract pathogen which, along with Streptococcus pneumoniae and Haemophilus influenzae, is classified as one of the most frequent bacterial etiological factors causing upper respiratory tract infections. In this manuscript, we report the existence of five tetracycline-resistant M. catarrhalis strains with confirmed presence of tetracycline resistance tetB gene. The strains were isolated from children under the age of three with signs of upper respiratory tract infections. Our ...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Epidemiology and antimicrobial susceptibility of Staphylococcus lugdunensis in a Greek tertiary-care hospital.
We report a retrospective study of all S. lugdunensis infection cases during a 3.5-year period in a large tertiary university hospital in Greece. S. lugdunensis was susceptible to most tested antibiotics, although a high resistance percentage was found to clindamycin (27%) and erythromycin (25%). The susceptibility rate to penicillin was 49%, much lower than previously reported elsewhere, indicating that penicillin may not be an optimal treatment choice for S. lugdunensis infections in our region. PMID: 32596739 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

In Vitro Activity of Ceftazidime/Avibactam Alone and in Combination With Fosfomycin and Carbapenems Against KPC-producing Klebsiella Pneumoniae.
Authors: Romanelli F, De Robertis A, Carone G, Dalfino L, Stufano M, Del Prete R, Mosca A Abstract KPC-producing Klebsiella pneumonia (KPC-Kp) represents a major therapeutic challenge in critically ill patients. Ceftazidime-avibactam (CAZ-AVI) is a new effective drug against KPC-Kp but, due to emerging resistant strains during monotherapy, the association with a second antibiotic has been advocated. Therefore, intravenous fosfomycin may be a possible choice for combination therapy. The aim of this study was to evaluate the in vitro susceptibility of CAZ-AVI alone and in combination with fosfomycin and carbapenems a...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

An alternative molecular approach for rapid and specific detection of clinically relevant bacteria causing prosthetic joint infections with bacteriophage K.
Authors: Šuster K, Podgornik A, Cör A Abstract Prosthetic joint infections (PJI) represent the most serious cause of prosthetic joint loosening, with high impact on patient life and health economics. Although not entirely reliable, the cultivation of intraoperative prosthetic tissue or synovial fluid remains the gold standard for determining the cause of PJI. Therefore, molecular methods are increasingly being introduced. The aim of this study was to optimize and assess an alternative molecular approach with the use of bacteriophage K for more rapid and specific detection of staphylococci in sonicate fl...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Clinical Utility of Platelet Count for Screening of Malaria.
Authors: Leli C, Di Matteo L, Gotta F, Vay D, Cavallo V, Mazzeo R, Busso S, Carrabba L, Rocchetti A Abstract Light microscopy, immunochromatographic rapid diagnostic tests and molecular methods are widely used to diagnose malaria. The aim of this study was to find variables among commonly available urgent blood tests to identify patients with low probability of having malaria in small-scale healthcare facilities in which none of the described methods is feasible within a short time. Diagnosis of malaria was made by examining both stained thick and thin blood films by light microscopy. Two hundred and eleven samples...
Source: New Microbiologica - June 10, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cryptococcosis in an HIV-negative, HCV positive, immunosenescent patient: a case report.
We report a case of systemic cryptococcal infection in an immunosenescent, HIV-negative patient. PMID: 32510160 [PubMed - in process] (Source: New Microbiologica)
Source: New Microbiologica - June 10, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Comparison of MycoPrep and the new MYCO-TB kit: rapid and efficient digestion and decontamination of respiratory specimens for the detection of Mycobacteria.
In conclusion, the MYCO-TB kit proved to be effective for the rapid digestion and decontamination of respiratory materials for the detection of Mycobacteria, making it possible to reduce the manual skills required and lower the risk of contamination. Longer decontamination time could be used for samples with a high level of contamination, such as those from cystic fibrosis patients. PMID: 32334488 [PubMed - in process] (Source: New Microbiologica)
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Surveillance of antimicrobial resistance in Serratia marcescens in Mexico.
Authors: González GM, de J Treviño-Rangel R, Campos CL, Villanueva-Lozano H, Bonifaz A, Franco-Cendejas R, López-Jácome LE, Bobadilla Del Valle M, Llaca-Díaz JM, Ayala-Gaytán JJ, Castañón-Olivares LR, Tinoco JC, Andrade A Abstract Antimicrobial resistance is a global public health threat. Therefore, surveillance studies are important tools to help direct antimicrobial use. The aim of this study was to investigate antimicrobial resistance in Serratia marcescens isolates collected in 2016-2017 at eight medical centers from two regions of Mexico. Selected S. marc...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cytotoxicity and internalization analysis of silicon nanowires in Buffalo Green Monkey cells: a preliminary study to evaluate the possibility of carrying viruses inside the cells.
Authors: Condò R, Leo M, Maiolo L, Convertino A, Sinibaldi F, Santucci R, Divizia A, Campanella V, La Rosa G, Colantoni A, Anselmi M, Divizia M Abstract Silicon nanowires (SiNWs) are attractive functional nanomaterials for biomedical applications. The ability to easily tune their size and density, potential biocompatibility, and knowledge of the chemical activation of SiNWs surface make them natural tools to interact with biological materials. We evaluated the possibility of exploiting SiNWs as carriers to introduce organic compounds into cells. The cellular toxicity and the internalization capacity of free-...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cryptococcosis in an HIV-negative, HCV positive, immunosenescent patient: a case report.
We report a case of systemic cryptococcal infection in an immunosenescent, HIV-negative patient. PMID: 32334491 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Fatal case of West Nile encephalitis associated with acute anteroseptal ST elevation myocardial infarction (STEMI): a case report.
We report a fatal case of WNV encephalitis associated with an acute anteroseptal ST elevation myocardial infarction. The patient was hospitalized with a fever, headache, nausea and vomiting. The physical examination revealed positive meningeal signs and an altered level of consciousness. High levels of cardiac enzymes (creatine phosphokinase/MB fraction, lactate dehydrogenase, myoglobin and cardiac troponin I) and ST elevation on electrocardiogram were found. Both CSF and urine samples were positive for WNV RNA. This case highlights the need of awareness of the possibility of a WNV-related myocardial infection, including m...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Dynamics of HIV-1 POL antibodies after ART in chronic HIV-1 infection.
In conclusion, a negative HIV-1 Pol and a seroreversion from positive to negative were observed in more than 15% of subjects included and were associated with a better immunological profile, suggesting a lower viral exposure to the immune system over time. PMID: 32310296 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Characterization of Human Cytomegalovirus UL16 and UL17 Transcripts.
Authors: Liu Z, Ma Y, Lu Y, Han L, Gao S, Zheng B, Qi Y, Huang Y, Ruan Q Abstract Up to now, the UL16-17 region of human cytomegalovirus (HCMV) has not been well characterized at the level of mRNA and protein, especially for the Han strain, the first clinical HCMV strain in China. In previous studies, three transcripts were detected from the UL16-17 region by northern blot analysis for Merlin strain. Transcriptions of UL16 and UL17 were also studied by 5' rapid amplification of cDNA ends (5'RACE) and deep sequencing for AD169 and Towne strains, respectively. However, details of 3' end of UL16 and UL17 transcripts h...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

The clinical impact of a multiplex real-time PCR system for microbiological diagnosis of sepsis: a mortality study.
Authors: Grijalva M, De La Torre K, Sánchez N Abstract To evaluate the impact of the use of a multiplex-real time PCR-based assay (SeptiFast MGrade ®) on in-hospital mortality in ICU sepsis patients. Demographic, clinical and microbiological data from ICU patients with suspected sepsis and available SeptiFast (SF) test results were gathered. The intervention group comprised patients in which SF indicated a clinical intervention; the non-intervention group included patients in whom SF result did not lead to any clinical intervention. The study looked at expected and bserved in-hospital mortality rates in ...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR.
Authors: Kukla R, Bolehovska R, Radocha J, Pliskova L, Zak P, Vrbacky F, Nekvindova J, Zemlickova H Abstract The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with communityacquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, bas...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Environmental factors associated with etiology of microbiologically confirmed reconstructive breast implant infections: impact on clinical management and treatment.
Authors: Franchelli S, Rossin A, Pesce M, Marchese A, De Maria A Abstract Even if wide differences exist in the incidence of Gram-negative infections following breast cancer implant reconstructions (2-20%), its occurrence needs to be considered to optimize antibiotic therapy, which is usually directed towards Gram-positive cocci. There is a general notion on the possible source of Gram-negative microorganisms during outdoor activities. For this reason, we administered a specific questionnaire to infected patients to investigate this aspect. In 450 consecutive implant reconstructions between January 1, 2016 and Marc...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

A preliminary study on the homology analysis of clinical Stenotrophomonas maltophilia isolates based on fatty acid profiles.
Authors: Zhao S, Wang L, Cao S, Liu J, Zhang Z, Li L, Chen F, Wu Q Abstract In order to investigate molecular typing and fatty acid methyl esters (FAMEs) typing of clinical Stenotrophomonas maltophilia (S.maltophilia) isolates based on Random Amplification Polymorphic DNA (RAPD) and Gas Chromatography-Mass Spectrometer (GC-MS) methods, we collected 35 drug-resistant S. maltophilia isolates from March to December 2017 in a comprehensive hospital. The VITEK 2 Compact System was used to determine bacterial antibiotic susceptibility. The analysis of molecular typing was performed by RAPD. GC-MS was used to obtain FAMEs...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Evaluation of CMV DNA in dried blood spot.
Authors: Bottino P, Balloco C, Rittà M, Bianco G, Sidoti F, Cavallo R, Costa C Abstract Cytomegalovirus is the primary viral cause of congenital infection. However, diagnosis may be difficult for clinical and technical reasons. Currently, evaluation of CMV DNA on dried blood spot (DBS) is an important instrument to define a congenital infection. The aim of this study was to identify a clinically and technically suitable diagnostic work-flow for CMV DNA evaluation on DBS. Sensitivity was not significantly influenced by storage time of up to 12 months and extraction technique; however, analysis in triplicate w...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Campylobacter jejuni bacteremia in Italian pediatric patients with acute lymphoblastic leukemia: Report of two cases.
We report two cases of C. jejuni bacteremia in Italian pediatric patients affected by acute lymphoblastic leukemia (ALL). Agreeing with the most recent epidemiological data, both clinical isolates showed a typical phenotypic antimicrobial resistance patterns with combined resistance to ciprofloxacin and tetracycline. To our knowledge, this is the first report of C. jejuni isolation from the blood of ALL pediatric patients in Italy, and it provides important epidemiological information on this rare infection. PMID: 32310303 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Accelerate Pheno ™ system in sepsis by Gram-negative pathogens: four months of hospital experience.
This study reports our experience with the Accelerate PhenoTM system (ACC) to guide management of patients with sepsis by Gram-negative pathogens. A diagnostic workflow, based on pathogen and resistance genes detection or ACC testing, was applied to 33 patients. Clinical and microbiological data were recorded, and analysis of broad-spectrum agents sparing was performed. Antimicrobial susceptibility results by ACC were available for 28 of 33 patients (84.85%). Among 434 microorganism-antimicrobial combinations, categorical agreement was 97.93%, very major errors 0.23%, major errors 1.15%, and minor errors 0.69%. Time to rep...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Bacterial network community in fecal and endoluminal Microbiota after colonoscopy.
This study aimed to evaluate the impact of bowel cleansing on the fecal microbiota network by highlighting differences between fecal microflora before and after colonoscopy, and luminal samples during colonoscopy. Fecal and luminal samples, previously analyzed by mean of Next-Generation Sequencing (NGS) for their bacterial abundance, were further processed by a method based on Artificial Neural Network (ANN) architecture. The bowel lavage had a strong effect on the intestinal microbiota network, leading to significant changes in the distribution of different bacterial hubs potentially involved in the microbiota homeostasis...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Clinical management of imported malaria in Italy: results from a national cross-sectional survey in 2015.
Authors: Lepore L, Vairo F, D'Abramo A, Grilli E, Corpolongo A, Scorzolini L, Nisii C, Calleri G, Castelli F, Chirianni A, Ippolito G, Nicastri E Abstract In Italy, malaria continues to be one of the most common imported parasitoses; therefore, continuous surveillance of epidemiological data and clinical management is needed. In 2016, the National Institute for Infectious Diseases 'Lazzaro Spallanzani' in Rome promoted a retrospective questionnaire-based survey to assess the clinical management of imported malaria cases in Italy in 2015. The questionnaire was sent to 104 Tropical and/or Infectious Diseases Units in...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

OXA-48 and NDM-1 Klebsiella pneumoniae of Sequence Type 101 from blood in a patient with travel history abroad, Italy.
Authors: Nucleo E, Marchetti VM, Mercato A, Quatela M, Villa L, Migliavacca R Abstract Klebsiella pneumoniae (KP) is an important pathogen involved in serious nosocomial infections all over the world. Here, we describe the first report on a blood-stream infection caused by an OXA- 48/NDM-1 ST101-KP, in Italy. The patient was an Italian woman, transferred from Cairo Hospital to a Neurosurgery ward in Cuneo (IT). The detection described here enhances the need for an effective National infection control strategy in Italy. PMID: 32118283 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Rotavirus molecular epidemiology in hospitalized patients, Northern Italy, 2015-2018.
Authors: Rovida F, Nepita EV, Giardina F, Piralla A, Campanini G, Baldanti F Abstract About 15,000 hospitalizations due to group A Rotavirus gastroenteritis (RVA) are recorded each year in Italy. In the present study, we report the seasonal distribution and molecular characterization of RVA in pediatric and adult hospitalized patients in the period September 2015-April 2018 in Pavia province, Lombardy Region. During the study period, stool samples of 1450 patients with acute gastroenteritis were analyzed and 122 were RVA positive, the majority belonging to pediatric patients (94.0%) while only a minority of patient...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Strongyloides stercoralis: a rare and severe presentation in a pregnant woman.
Authors: Beltrán Rosel A, Sanjoaquín Conde I, Pérez Muñoz A, Alcalá PM, Marta CB, Irigoyen von Sierakowski A Abstract Strongyloides stercoralis is a parasitic nematode with a worldwide distribution. It can go from an asymptomatic infection to a life-threatening hyperinfection syndrome. Here, we report a case of intestinal obstruction due to S. stercoralis in a pregnant woman. This condition, as well as severe strongyloidiasis in pregnant women, is seldomly reported. In this case, Human Tlymphotropic Virus 1 (HTLV-1) coinfection was confirmed, a well-known risk factor for a more se...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Candida palmioleophila isolation in Italy from two cases of systemic infection, after a CHROMagar and Vitek system mis-identification as C.albicans.
Authors: Casagrande Pierantoni D, Bernardo M, Mallardo E, Carannante N, Attanasio V, Corte L, Roscini L, Di Fiore L, Tascini C, Cardinali G Abstract A correct, fast, reliable identification method is pivotal in nosocomial environments to guide treatment strategies, whereas misidentification might lead to treatment failure. For routine identifications the Vitek system and CHROMagar are widely used but not always reliable, especially now with an increasing number of new emerging fungal pathogens that need careful identification. Here we describe two cases of candidemia, due to Candida palmioleophila previously miside...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Differences between Community- and Hospital-acquired urinary tract infections in a tertiary care hospital.
We reported a general increase of unnecessary urine cultures in both community and hospital patients. The most representative microorganism isolated from Community (58.2%) and Hospital (47.6%) was E. coli. UTIs causative bacteria in hospitalized patients was more than twice as resistant to Trimetoprim/sulphamethoxazole (OR 2.26) and Imipenem (OR 2.56), for Gram-positive and Gram-negative, respectively, than in Community patients. Nitrofurantoin was the only agent without differences in resistance rate between community and hospital UTIs. Therefore, physicians could use it as a definitive therapy for uncomplicated cystitis ...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Efficacy, Safety, and Predictors of Direct-acting antivirals in Hepatitis C Virus Patients with Heterogeneous Liver Diseases.
Authors: De Pace V, Morelli MC, Ravaioli M, Maggi F, Galli S, Vero V, Re MC, Cescon M, Pistello M Abstract Safety, efficacy, and predictor factors of sustained-virological-response after 24 weeks of new directacting antivirals were evaluated in hepatitis C virus patients with different stages of hepatic disease. 260 patients, median age 60 years, of whom 48.1% cirrhotics, 17.7% liver transplant recipients, and 45.7% naïve were treated with Sofosbuvir+Ribavirine, Sofosbuvir+Simeprevir±Ribavirine, Sofosbuvir+Daclatasvir±Ribavirine, Sofosbuvir+Ledispavir±Ribavirine, Ombitasvir/Paritaprevir/Ri...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Antimicrobial susceptibility and prevalence of extended-spectrum beta-lactamases in clinical strains of Klebsiella pneumoniae isolated from pediatric and adult patients of two Polish hospitals.
Authors: Mrowiec P, Klesiewicz K, Małek M, Skiba-Kurek I, Sowa-Sierant I, Skałkowska M, Budak A, Karczewska E Abstract Klebsiella pneumoniae due to the presence of multiple antibiotic resistance mechanisms is one of the most threatening human pathogens nowadays. The aim of the study was to characterize antimicrobial susceptibility, presence of resistance mechanisms and the prevalence of selected genes encoding ESBLs in 170 K. pneumoniae isolates recovered from children and adults hospitalized in two Polish medical centers from 2008 to 2015. The phenotypic identification of strains was confirmed by amplification o...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Fecal Microbial Transplantation impact on gut microbiota composition and metabolome, microbial translocation and T-lymphocyte immune activation in recurrent Clostridium difficile infection patients.
Authors: Bruno G, Gagliardi A, Oliva A, Trancassini M, Macone A, Cicerone C, D'Abramo A, Iebba V, Auria S, Bonfiglio G, Zingaropoli MA, D'Ettorre G, Badiali D, Vullo V, Corazziari ES, Schippa S Abstract This short communication reports the preliminary results of Fecal Microbial Transplantation (FMT) impact on microbiota, microbial translocation (MT), and immune activation in four recurrent Clostridium difficile infection (R-CDI) patients. After FMT a restore of gut microbiota composition with a significant increase of fecal acetyl-putrescine and spermidine and fecal acetate and butyrate, a decrease of immune activa...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Alloscardovia Omnicolens emerging presence in premature rupture of membranes.
Authors: Cardona-Benavides I, Puertas-Prieto A, Pinilla-Martín FJ, Navarro-Marí JM, Gutiérrez-Fernández J Abstract Alloscardovia omnicolens is a recently-reported microorganism with unknown pathogenic implications. It has been isolated in various clinical localizations but not in the endocervix. We isolated A. omnicolens in an endocervical sample from a 31-yr-old patient with preterm premature rupture of membranes (PPROM) in week 33+3 of pregnancy. The main risk of PPROM is prematurity and the possibility of developing infectious chorioamnionitis, which can be lethal for the mother and n...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Topical Rifampicin versus Povidone-Iodine for the Prevention of Incisional Surgical Site Infections Following Benign Gynecologic Surgery: A Prospective, Randomized, Controlled Trial.
Authors: Karuserci ÖK, Sucu S, Özcan HÇ, Tepe NB, Uğur MG, Güneyligil T, Balat Ö Abstract In this prospective, randomized, controlled study we investigated the effect of subcutaneous rifampicin and povidone-iodine irrigation on incisional surgical site infection. Superficial incisional surgical site infection (SSI) following gynecologic surgery is a serious problem for both patient and surgeon in terms of increased morbidity, length of hospital stay, anxiety, and costs. Three hundred patients scheduled for abdominal surgery due to various benign gynecological pathologies were randomly as...
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Subcutaneous Human Dirofilariosis By D. Repens In South Italy: A Case Report.
Authors: Brindicci G, Santoro CR, Signorile F, Leone A, Di Ciaula G, Monno L, Angarano G Abstract Human dirofilariosis is a zoonosis caused by different Dirofilaria species: D. repens, D. immitis, D. tenuis and D. ursi, thin nematodes belonging to the Onchocercidae family, whose larval stages are generally found in the natural (felines and canids) or accidental (human) definitive host. In Europe, human infection is rare, even in areas considered endemic such as Spain or Italy. In this paper we describe the case of an 82-year-old woman living in Modugno (Bari municipality), who came to our observation for a subcutan...
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Plasmodium vivax exflagellated microgametes in human peripheral blood: a potential diagnostic dilemma.
Authors: Camarlinghi G, Parisio EM, Nardone M, Mancini F, Ciervo A, Boccolini D, Mattei R Abstract Both malaria and relapsing fever Borrelia are infectious diseases characterized by fever, headache, myalgia, hepatosplenomegaly and tendency to relapse. Exflagellation of microgametocyte in malarial parasites is seen only in the definitive host, i.e., mosquitoes. Here we report an unusual case of a 23-year-old man who presented Plasmodium vivax infection with multiple exflagellated microgametes in the peripheral blood smear. PMID: 31524945 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research