Tetracycline resistance in Moraxella catarrhalis clinical strains isolated in Poland.
Authors: Król-Turmińska K, Olender A, Bogut A Abstract Moraxella catarrhalis is considered an important, exclusively human respiratory tract pathogen which, along with Streptococcus pneumoniae and Haemophilus influenzae, is classified as one of the most frequent bacterial etiological factors causing upper respiratory tract infections. In this manuscript, we report the existence of five tetracycline-resistant M. catarrhalis strains with confirmed presence of tetracycline resistance tetB gene. The strains were isolated from children under the age of three with signs of upper respiratory tract infections. Our ...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Epidemiology and antimicrobial susceptibility of Staphylococcus lugdunensis in a Greek tertiary-care hospital.
We report a retrospective study of all S. lugdunensis infection cases during a 3.5-year period in a large tertiary university hospital in Greece. S. lugdunensis was susceptible to most tested antibiotics, although a high resistance percentage was found to clindamycin (27%) and erythromycin (25%). The susceptibility rate to penicillin was 49%, much lower than previously reported elsewhere, indicating that penicillin may not be an optimal treatment choice for S. lugdunensis infections in our region. PMID: 32596739 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

In Vitro Activity of Ceftazidime/Avibactam Alone and in Combination With Fosfomycin and Carbapenems Against KPC-producing Klebsiella Pneumoniae.
Authors: Romanelli F, De Robertis A, Carone G, Dalfino L, Stufano M, Del Prete R, Mosca A Abstract KPC-producing Klebsiella pneumonia (KPC-Kp) represents a major therapeutic challenge in critically ill patients. Ceftazidime-avibactam (CAZ-AVI) is a new effective drug against KPC-Kp but, due to emerging resistant strains during monotherapy, the association with a second antibiotic has been advocated. Therefore, intravenous fosfomycin may be a possible choice for combination therapy. The aim of this study was to evaluate the in vitro susceptibility of CAZ-AVI alone and in combination with fosfomycin and carbapenems a...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

An alternative molecular approach for rapid and specific detection of clinically relevant bacteria causing prosthetic joint infections with bacteriophage K.
Authors: Šuster K, Podgornik A, Cör A Abstract Prosthetic joint infections (PJI) represent the most serious cause of prosthetic joint loosening, with high impact on patient life and health economics. Although not entirely reliable, the cultivation of intraoperative prosthetic tissue or synovial fluid remains the gold standard for determining the cause of PJI. Therefore, molecular methods are increasingly being introduced. The aim of this study was to optimize and assess an alternative molecular approach with the use of bacteriophage K for more rapid and specific detection of staphylococci in sonicate fl...
Source: New Microbiologica - July 1, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Clinical Utility of Platelet Count for Screening of Malaria.
Authors: Leli C, Di Matteo L, Gotta F, Vay D, Cavallo V, Mazzeo R, Busso S, Carrabba L, Rocchetti A Abstract Light microscopy, immunochromatographic rapid diagnostic tests and molecular methods are widely used to diagnose malaria. The aim of this study was to find variables among commonly available urgent blood tests to identify patients with low probability of having malaria in small-scale healthcare facilities in which none of the described methods is feasible within a short time. Diagnosis of malaria was made by examining both stained thick and thin blood films by light microscopy. Two hundred and eleven samples...
Source: New Microbiologica - June 10, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cryptococcosis in an HIV-negative, HCV positive, immunosenescent patient: a case report.
We report a case of systemic cryptococcal infection in an immunosenescent, HIV-negative patient. PMID: 32510160 [PubMed - in process] (Source: New Microbiologica)
Source: New Microbiologica - June 10, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Comparison of MycoPrep and the new MYCO-TB kit: rapid and efficient digestion and decontamination of respiratory specimens for the detection of Mycobacteria.
In conclusion, the MYCO-TB kit proved to be effective for the rapid digestion and decontamination of respiratory materials for the detection of Mycobacteria, making it possible to reduce the manual skills required and lower the risk of contamination. Longer decontamination time could be used for samples with a high level of contamination, such as those from cystic fibrosis patients. PMID: 32334488 [PubMed - in process] (Source: New Microbiologica)
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Surveillance of antimicrobial resistance in Serratia marcescens in Mexico.
Authors: González GM, de J Treviño-Rangel R, Campos CL, Villanueva-Lozano H, Bonifaz A, Franco-Cendejas R, López-Jácome LE, Bobadilla Del Valle M, Llaca-Díaz JM, Ayala-Gaytán JJ, Castañón-Olivares LR, Tinoco JC, Andrade A Abstract Antimicrobial resistance is a global public health threat. Therefore, surveillance studies are important tools to help direct antimicrobial use. The aim of this study was to investigate antimicrobial resistance in Serratia marcescens isolates collected in 2016-2017 at eight medical centers from two regions of Mexico. Selected S. marc...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cytotoxicity and internalization analysis of silicon nanowires in Buffalo Green Monkey cells: a preliminary study to evaluate the possibility of carrying viruses inside the cells.
Authors: Condò R, Leo M, Maiolo L, Convertino A, Sinibaldi F, Santucci R, Divizia A, Campanella V, La Rosa G, Colantoni A, Anselmi M, Divizia M Abstract Silicon nanowires (SiNWs) are attractive functional nanomaterials for biomedical applications. The ability to easily tune their size and density, potential biocompatibility, and knowledge of the chemical activation of SiNWs surface make them natural tools to interact with biological materials. We evaluated the possibility of exploiting SiNWs as carriers to introduce organic compounds into cells. The cellular toxicity and the internalization capacity of free-...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Cryptococcosis in an HIV-negative, HCV positive, immunosenescent patient: a case report.
We report a case of systemic cryptococcal infection in an immunosenescent, HIV-negative patient. PMID: 32334491 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Fatal case of West Nile encephalitis associated with acute anteroseptal ST elevation myocardial infarction (STEMI): a case report.
We report a fatal case of WNV encephalitis associated with an acute anteroseptal ST elevation myocardial infarction. The patient was hospitalized with a fever, headache, nausea and vomiting. The physical examination revealed positive meningeal signs and an altered level of consciousness. High levels of cardiac enzymes (creatine phosphokinase/MB fraction, lactate dehydrogenase, myoglobin and cardiac troponin I) and ST elevation on electrocardiogram were found. Both CSF and urine samples were positive for WNV RNA. This case highlights the need of awareness of the possibility of a WNV-related myocardial infection, including m...
Source: New Microbiologica - April 28, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Dynamics of HIV-1 POL antibodies after ART in chronic HIV-1 infection.
In conclusion, a negative HIV-1 Pol and a seroreversion from positive to negative were observed in more than 15% of subjects included and were associated with a better immunological profile, suggesting a lower viral exposure to the immune system over time. PMID: 32310296 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Characterization of Human Cytomegalovirus UL16 and UL17 Transcripts.
Authors: Liu Z, Ma Y, Lu Y, Han L, Gao S, Zheng B, Qi Y, Huang Y, Ruan Q Abstract Up to now, the UL16-17 region of human cytomegalovirus (HCMV) has not been well characterized at the level of mRNA and protein, especially for the Han strain, the first clinical HCMV strain in China. In previous studies, three transcripts were detected from the UL16-17 region by northern blot analysis for Merlin strain. Transcriptions of UL16 and UL17 were also studied by 5' rapid amplification of cDNA ends (5'RACE) and deep sequencing for AD169 and Towne strains, respectively. However, details of 3' end of UL16 and UL17 transcripts h...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

The clinical impact of a multiplex real-time PCR system for microbiological diagnosis of sepsis: a mortality study.
Authors: Grijalva M, De La Torre K, Sánchez N Abstract To evaluate the impact of the use of a multiplex-real time PCR-based assay (SeptiFast MGrade ®) on in-hospital mortality in ICU sepsis patients. Demographic, clinical and microbiological data from ICU patients with suspected sepsis and available SeptiFast (SF) test results were gathered. The intervention group comprised patients in which SF indicated a clinical intervention; the non-intervention group included patients in whom SF result did not lead to any clinical intervention. The study looked at expected and bserved in-hospital mortality rates in ...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR.
Authors: Kukla R, Bolehovska R, Radocha J, Pliskova L, Zak P, Vrbacky F, Nekvindova J, Zemlickova H Abstract The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with communityacquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, bas...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Environmental factors associated with etiology of microbiologically confirmed reconstructive breast implant infections: impact on clinical management and treatment.
Authors: Franchelli S, Rossin A, Pesce M, Marchese A, De Maria A Abstract Even if wide differences exist in the incidence of Gram-negative infections following breast cancer implant reconstructions (2-20%), its occurrence needs to be considered to optimize antibiotic therapy, which is usually directed towards Gram-positive cocci. There is a general notion on the possible source of Gram-negative microorganisms during outdoor activities. For this reason, we administered a specific questionnaire to infected patients to investigate this aspect. In 450 consecutive implant reconstructions between January 1, 2016 and Marc...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

A preliminary study on the homology analysis of clinical Stenotrophomonas maltophilia isolates based on fatty acid profiles.
Authors: Zhao S, Wang L, Cao S, Liu J, Zhang Z, Li L, Chen F, Wu Q Abstract In order to investigate molecular typing and fatty acid methyl esters (FAMEs) typing of clinical Stenotrophomonas maltophilia (S.maltophilia) isolates based on Random Amplification Polymorphic DNA (RAPD) and Gas Chromatography-Mass Spectrometer (GC-MS) methods, we collected 35 drug-resistant S. maltophilia isolates from March to December 2017 in a comprehensive hospital. The VITEK 2 Compact System was used to determine bacterial antibiotic susceptibility. The analysis of molecular typing was performed by RAPD. GC-MS was used to obtain FAMEs...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Evaluation of CMV DNA in dried blood spot.
Authors: Bottino P, Balloco C, Rittà M, Bianco G, Sidoti F, Cavallo R, Costa C Abstract Cytomegalovirus is the primary viral cause of congenital infection. However, diagnosis may be difficult for clinical and technical reasons. Currently, evaluation of CMV DNA on dried blood spot (DBS) is an important instrument to define a congenital infection. The aim of this study was to identify a clinically and technically suitable diagnostic work-flow for CMV DNA evaluation on DBS. Sensitivity was not significantly influenced by storage time of up to 12 months and extraction technique; however, analysis in triplicate w...
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Campylobacter jejuni bacteremia in Italian pediatric patients with acute lymphoblastic leukemia: Report of two cases.
We report two cases of C. jejuni bacteremia in Italian pediatric patients affected by acute lymphoblastic leukemia (ALL). Agreeing with the most recent epidemiological data, both clinical isolates showed a typical phenotypic antimicrobial resistance patterns with combined resistance to ciprofloxacin and tetracycline. To our knowledge, this is the first report of C. jejuni isolation from the blood of ALL pediatric patients in Italy, and it provides important epidemiological information on this rare infection. PMID: 32310303 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - April 22, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Accelerate Pheno ™ system in sepsis by Gram-negative pathogens: four months of hospital experience.
This study reports our experience with the Accelerate PhenoTM system (ACC) to guide management of patients with sepsis by Gram-negative pathogens. A diagnostic workflow, based on pathogen and resistance genes detection or ACC testing, was applied to 33 patients. Clinical and microbiological data were recorded, and analysis of broad-spectrum agents sparing was performed. Antimicrobial susceptibility results by ACC were available for 28 of 33 patients (84.85%). Among 434 microorganism-antimicrobial combinations, categorical agreement was 97.93%, very major errors 0.23%, major errors 1.15%, and minor errors 0.69%. Time to rep...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Bacterial network community in fecal and endoluminal Microbiota after colonoscopy.
This study aimed to evaluate the impact of bowel cleansing on the fecal microbiota network by highlighting differences between fecal microflora before and after colonoscopy, and luminal samples during colonoscopy. Fecal and luminal samples, previously analyzed by mean of Next-Generation Sequencing (NGS) for their bacterial abundance, were further processed by a method based on Artificial Neural Network (ANN) architecture. The bowel lavage had a strong effect on the intestinal microbiota network, leading to significant changes in the distribution of different bacterial hubs potentially involved in the microbiota homeostasis...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Clinical management of imported malaria in Italy: results from a national cross-sectional survey in 2015.
Authors: Lepore L, Vairo F, D'Abramo A, Grilli E, Corpolongo A, Scorzolini L, Nisii C, Calleri G, Castelli F, Chirianni A, Ippolito G, Nicastri E Abstract In Italy, malaria continues to be one of the most common imported parasitoses; therefore, continuous surveillance of epidemiological data and clinical management is needed. In 2016, the National Institute for Infectious Diseases 'Lazzaro Spallanzani' in Rome promoted a retrospective questionnaire-based survey to assess the clinical management of imported malaria cases in Italy in 2015. The questionnaire was sent to 104 Tropical and/or Infectious Diseases Units in...
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

OXA-48 and NDM-1 Klebsiella pneumoniae of Sequence Type 101 from blood in a patient with travel history abroad, Italy.
Authors: Nucleo E, Marchetti VM, Mercato A, Quatela M, Villa L, Migliavacca R Abstract Klebsiella pneumoniae (KP) is an important pathogen involved in serious nosocomial infections all over the world. Here, we describe the first report on a blood-stream infection caused by an OXA- 48/NDM-1 ST101-KP, in Italy. The patient was an Italian woman, transferred from Cairo Hospital to a Neurosurgery ward in Cuneo (IT). The detection described here enhances the need for an effective National infection control strategy in Italy. PMID: 32118283 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - March 3, 2020 Category: Microbiology Tags: New Microbiol Source Type: research

Rotavirus molecular epidemiology in hospitalized patients, Northern Italy, 2015-2018.
Authors: Rovida F, Nepita EV, Giardina F, Piralla A, Campanini G, Baldanti F Abstract About 15,000 hospitalizations due to group A Rotavirus gastroenteritis (RVA) are recorded each year in Italy. In the present study, we report the seasonal distribution and molecular characterization of RVA in pediatric and adult hospitalized patients in the period September 2015-April 2018 in Pavia province, Lombardy Region. During the study period, stool samples of 1450 patients with acute gastroenteritis were analyzed and 122 were RVA positive, the majority belonging to pediatric patients (94.0%) while only a minority of patient...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Strongyloides stercoralis: a rare and severe presentation in a pregnant woman.
Authors: Beltrán Rosel A, Sanjoaquín Conde I, Pérez Muñoz A, Alcalá PM, Marta CB, Irigoyen von Sierakowski A Abstract Strongyloides stercoralis is a parasitic nematode with a worldwide distribution. It can go from an asymptomatic infection to a life-threatening hyperinfection syndrome. Here, we report a case of intestinal obstruction due to S. stercoralis in a pregnant woman. This condition, as well as severe strongyloidiasis in pregnant women, is seldomly reported. In this case, Human Tlymphotropic Virus 1 (HTLV-1) coinfection was confirmed, a well-known risk factor for a more se...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Candida palmioleophila isolation in Italy from two cases of systemic infection, after a CHROMagar and Vitek system mis-identification as C.albicans.
Authors: Casagrande Pierantoni D, Bernardo M, Mallardo E, Carannante N, Attanasio V, Corte L, Roscini L, Di Fiore L, Tascini C, Cardinali G Abstract A correct, fast, reliable identification method is pivotal in nosocomial environments to guide treatment strategies, whereas misidentification might lead to treatment failure. For routine identifications the Vitek system and CHROMagar are widely used but not always reliable, especially now with an increasing number of new emerging fungal pathogens that need careful identification. Here we describe two cases of candidemia, due to Candida palmioleophila previously miside...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Differences between Community- and Hospital-acquired urinary tract infections in a tertiary care hospital.
We reported a general increase of unnecessary urine cultures in both community and hospital patients. The most representative microorganism isolated from Community (58.2%) and Hospital (47.6%) was E. coli. UTIs causative bacteria in hospitalized patients was more than twice as resistant to Trimetoprim/sulphamethoxazole (OR 2.26) and Imipenem (OR 2.56), for Gram-positive and Gram-negative, respectively, than in Community patients. Nitrofurantoin was the only agent without differences in resistance rate between community and hospital UTIs. Therefore, physicians could use it as a definitive therapy for uncomplicated cystitis ...
Source: New Microbiologica - December 10, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Efficacy, Safety, and Predictors of Direct-acting antivirals in Hepatitis C Virus Patients with Heterogeneous Liver Diseases.
Authors: De Pace V, Morelli MC, Ravaioli M, Maggi F, Galli S, Vero V, Re MC, Cescon M, Pistello M Abstract Safety, efficacy, and predictor factors of sustained-virological-response after 24 weeks of new directacting antivirals were evaluated in hepatitis C virus patients with different stages of hepatic disease. 260 patients, median age 60 years, of whom 48.1% cirrhotics, 17.7% liver transplant recipients, and 45.7% naïve were treated with Sofosbuvir+Ribavirine, Sofosbuvir+Simeprevir±Ribavirine, Sofosbuvir+Daclatasvir±Ribavirine, Sofosbuvir+Ledispavir±Ribavirine, Ombitasvir/Paritaprevir/Ri...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Antimicrobial susceptibility and prevalence of extended-spectrum beta-lactamases in clinical strains of Klebsiella pneumoniae isolated from pediatric and adult patients of two Polish hospitals.
Authors: Mrowiec P, Klesiewicz K, Małek M, Skiba-Kurek I, Sowa-Sierant I, Skałkowska M, Budak A, Karczewska E Abstract Klebsiella pneumoniae due to the presence of multiple antibiotic resistance mechanisms is one of the most threatening human pathogens nowadays. The aim of the study was to characterize antimicrobial susceptibility, presence of resistance mechanisms and the prevalence of selected genes encoding ESBLs in 170 K. pneumoniae isolates recovered from children and adults hospitalized in two Polish medical centers from 2008 to 2015. The phenotypic identification of strains was confirmed by amplification o...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Fecal Microbial Transplantation impact on gut microbiota composition and metabolome, microbial translocation and T-lymphocyte immune activation in recurrent Clostridium difficile infection patients.
Authors: Bruno G, Gagliardi A, Oliva A, Trancassini M, Macone A, Cicerone C, D'Abramo A, Iebba V, Auria S, Bonfiglio G, Zingaropoli MA, D'Ettorre G, Badiali D, Vullo V, Corazziari ES, Schippa S Abstract This short communication reports the preliminary results of Fecal Microbial Transplantation (FMT) impact on microbiota, microbial translocation (MT), and immune activation in four recurrent Clostridium difficile infection (R-CDI) patients. After FMT a restore of gut microbiota composition with a significant increase of fecal acetyl-putrescine and spermidine and fecal acetate and butyrate, a decrease of immune activa...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Alloscardovia Omnicolens emerging presence in premature rupture of membranes.
Authors: Cardona-Benavides I, Puertas-Prieto A, Pinilla-Martín FJ, Navarro-Marí JM, Gutiérrez-Fernández J Abstract Alloscardovia omnicolens is a recently-reported microorganism with unknown pathogenic implications. It has been isolated in various clinical localizations but not in the endocervix. We isolated A. omnicolens in an endocervical sample from a 31-yr-old patient with preterm premature rupture of membranes (PPROM) in week 33+3 of pregnancy. The main risk of PPROM is prematurity and the possibility of developing infectious chorioamnionitis, which can be lethal for the mother and n...
Source: New Microbiologica - October 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Topical Rifampicin versus Povidone-Iodine for the Prevention of Incisional Surgical Site Infections Following Benign Gynecologic Surgery: A Prospective, Randomized, Controlled Trial.
Authors: Karuserci ÖK, Sucu S, Özcan HÇ, Tepe NB, Uğur MG, Güneyligil T, Balat Ö Abstract In this prospective, randomized, controlled study we investigated the effect of subcutaneous rifampicin and povidone-iodine irrigation on incisional surgical site infection. Superficial incisional surgical site infection (SSI) following gynecologic surgery is a serious problem for both patient and surgeon in terms of increased morbidity, length of hospital stay, anxiety, and costs. Three hundred patients scheduled for abdominal surgery due to various benign gynecological pathologies were randomly as...
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Subcutaneous Human Dirofilariosis By D. Repens In South Italy: A Case Report.
Authors: Brindicci G, Santoro CR, Signorile F, Leone A, Di Ciaula G, Monno L, Angarano G Abstract Human dirofilariosis is a zoonosis caused by different Dirofilaria species: D. repens, D. immitis, D. tenuis and D. ursi, thin nematodes belonging to the Onchocercidae family, whose larval stages are generally found in the natural (felines and canids) or accidental (human) definitive host. In Europe, human infection is rare, even in areas considered endemic such as Spain or Italy. In this paper we describe the case of an 82-year-old woman living in Modugno (Bari municipality), who came to our observation for a subcutan...
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Plasmodium vivax exflagellated microgametes in human peripheral blood: a potential diagnostic dilemma.
Authors: Camarlinghi G, Parisio EM, Nardone M, Mancini F, Ciervo A, Boccolini D, Mattei R Abstract Both malaria and relapsing fever Borrelia are infectious diseases characterized by fever, headache, myalgia, hepatosplenomegaly and tendency to relapse. Exflagellation of microgametocyte in malarial parasites is seen only in the definitive host, i.e., mosquitoes. Here we report an unusual case of a 23-year-old man who presented Plasmodium vivax infection with multiple exflagellated microgametes in the peripheral blood smear. PMID: 31524945 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

H. pylori-miRNA interaction in gastric cancer tissues: First prospective study from Turkey.
Authors: Demiryas S, Kocazeybek B, Demirci M, Caliskan R, Kepil N, Uysal HK, Dinc HO, Saribas S, Ergin S, Erzin Y, Tasci İ, Erdamar S, Demirdag C, Tokman HB Abstract Helicobacter pylori (H. pylori) is involved in the etiology of gastric cancer (GC). miRNAs are short RNAs that regulate gene expression by marking mRNAs for degradation. miRNAs are involved in tumorigenesis, metastasis, and cell proliferation. We aimed to investigate the miRNA expression profiles of tissues from H. pylori (+) and (-) GC patients. Forty GC patients, 20 H. pylori (+) and 20 H. pylori (-), and a healthy control group were included. The m...
Source: New Microbiologica - September 17, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Factors associated with vitamin D deficiency in HIV-1 infected patients on combination antiretroviral therapy: a case-control study.
Authors: Calza L, di Pietro G, Colangeli V, Borderi M, Zaghi I, Malosso P, Bon I, Re MC, Viale P Abstract The aim of our study was to assess risk factors associated with vitamin D deficiency among HIV-1-infected patients on combination antiretroviral therapy (cART). A retrospective, case-control study was conducted to assess risk factors associated with vitamin D deficiency among HIV-1-infected adults on stable cART. Vitamin D deficiency was defined as 25-OH vitamin D concentration 600 cells/mmc and current HIV RNA
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Early improvement of glycaemic control after virus clearance in patients with chronic hepatitis C and severe liver fibrosis: a cohort study.
Authors: Lanini S, Bartolini B, Taibi C, Agresta A, Garbuglia AR, Montaldo C, Pisapia R, D'Offizi G, Scognamiglio P, Capobianchi MR, Zumla A, Ippolito G Abstract HCV has been recognized as the cause of chronic hepatitis C (CHC) since 1990. CHC is associated with progressive liver damage and extrahepatic conditions. Direct antiviral agents (DAAs), approved in 2014, have shown effectiveness in eradicating HCV in most patients. However, little is known about the effect of viral eradication on hepatic and extra-hepatic damage. We performed a historical cohort study of patients with HCV-related liver diseases who achiev...
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Occurrence of two Norovirus outbreaks in the same cafeteria in one week.
Authors: Monini M, Ostanello F, Vignolo E, Pagani E, Gamper S, Spertini S, Masi E, Rabini M, Stenico A, Poznanski E, Di Bartolo I Abstract In October 2017, two outbreaks of gastroenteritis (GE) occurred among patrons of a cafeteria in Italy in one week. Virological and bacteria investigations on stool samples, environment and food were conducted to identify the infectious agents and the possible source of infection. Forty-five cases occurred in the two outbreaks, including 13 laboratory-confirmed cases of norovirus GI. Nine staff members were interviewed, six were confirmed positive for NoV GI and 3 experienced GE ...
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

SLAM Project - Second-Level Diagnostic Assessment: Multidisciplinary approach to HIV Patients.
Authors: Di Biagio A, Bonfanti P, Madeddu G, De Socio G, Maggi P, Vichi F, Martinelli C, Menzaghi B, Orofino G, Squillace N, Ricci E, Acone B, Quirino T, C.I.S.A.I. Group Abstract The aim of the study is to propose a multidimensional second-level diagnostic assessment to allow follow-up in the event physicians observe the presence of risk factors and/or active comorbidities in HIV-infected patients. To develop our proposal, we chose the Delphi method that has been used for about 30 years in the healthcare field. The CISAI Group (Coordinamento Italiano per lo Studio dell'Allergia in Infezione da HIV) conducted this ...
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Antibiotic susceptibilities and genetic variations in macrolide resistance genes of Ureaplasma spp. isolated in China.
Authors: Song T, Huang J, Liu Z, Zhang Y, Kong Y, Ruan Z Abstract Macrolides are widely used for the treatment of Ureaplasma spp. infection. The aim of this study was to investigate possible genetic resistance determinants in Ureaplasma spp. isolates. A total of eleven macrolide-resistant Ureaplasma spp. isolates, recovered from urogenital specimens, were investigated for genetic mechanisms of macrolide resistance. The 23S rRNA operons, as well as L4 and L22 ribosomal protein genes, were amplified and sequenced. Our study identified that the mutation A2066G in the 23S rRNA and four mutations (G361T, A406G, C422T an...
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Cranial nerve palsy: an unusual presentation of a fulminant fungal disease.
We report a case of rhino-orbital mucormycosis, which commenced as cranial nerve palsy, in a woman with ignored diabetes mellitus. Mucormycosis should therefore be taken into consideration during the differential diagnosis of cranial nerve palsy, especially in diabetic or immunocompromised patients. PMID: 31305937 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Uncommon testicular localization of Disseminated TB: a case report from Mozambique.
We reported the case of a 45-year-old male, HIV positive, naïve to antiretroviral treatment, admitted to the Department of Medicine of the Central Hospital of Beira to investigate chronic enlargement of the testicles. PMID: 31305938 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - July 16, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Point-of-care testing for hepatitis C virus infection at an Italian dental clinic: portrait of the pilot study population.
This study aimed to determine the socio-demographic profile of subjects screened for HCV virus in a dental clinic to acquire useful information for future campaigns of prevention. An easy, free-of-charge, screen salivary test was offered to patients referred to the dental clinic of San Raffaele Scientific Research Hospital in Milan, Italy for dental procedures. These patients were also asked to complete an anonymous questionnaire on demographics and risk behaviours. A total of 1388 of 2097 (66.19%) questionnaires were evaluable. The demographics of the population responding to this initiative was primarily Italians citizen...
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Syphilis in a high-density urban area in the North of Italy.
In this study, we evaluated the presence and characteristics of syphilis in a group of patients attending an STI Clinic in the North of Italy. A retrospective study was carried out, analysing the data from the 5609 subjects who attended the STI Clinic of St. Orsola-Malpighi Hospital (Bologna) for syphilis screening from January 2016 to December 2017. Globally, 692 patients (12.3%) were found positive for treponemal tests, with a significant difference between males and females (16.6% vs 4.1%; P
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Predictors of time to sputum smear conversion in patients with pulmonary tuberculosis under treatment.
This study aims to ascertain the time to sputum conversion in patients with smear-positive P-TB under treatment, and the variables associated with time to smear conversion. We retrospectively evaluated the time to sputum smear conversion of 89 patients with smearpositive P-TB undergoing treatment at the S. Orsola-Malpighi University Hospital, Bologna (Italy), a referral centre for the diagnosis of TB. Multivariate Cox regression analysis was performed to document variables independently associated with time to conversion. Median time to sputum smear conversion was 24 days (IQR 12-54); the sputum smear converted within the ...
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

A Position Paper on IgM-Enriched Intravenous Immunoglobulin Adjunctive Therapy in Severe Acute Bacterial Infections: The TO-PIRO SCORE Proposal.
Authors: De Rosa FG, Corcione S, Tascini C, Pasero D, Rocchetti A, Massaia M, Berlot G, Solidoro P, Girardis M Abstract IgM-enriched immunoglobulins (IgM) may be useful in patients with severe acute bacterial infections. The evidence for the administration of IgM is not extensive and a definitive consensus has never been reached on its best use in patients with acute infections as well as in critically ill patients. However, the official indication in several countries, including Italy, is quite wide and mainly refers to supportive treatment of patients with acute severe bacterial infections. A multidisciplinary me...
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Nonstructural Protein NP1 of Human Bocavirus 1 suppresses the growth of A549 cell by promoting autophagy.
This study provides insight into further elucidating the replication mechanism of HBoV1. PMID: 31157401 [PubMed - as supplied by publisher] (Source: New Microbiologica)
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Hepatitis A outbreak affecting men who have sex with men (MSM) in South Italy.
Authors: Mauro MV, Greco F, Tenuta R, Apuzzo G, Costantino A, Bruni R, Equestre M, Ciccaglione AR, Giraldi C, Mastroianni A Abstract From April to October 2017, 27 cases of Hepatitis A (HA), 22 male and 5 female, were reported in Cosenza (South Italy). The median age of cases was 32 years (range 3-49 years). Out of 21 male adults, 14 were identified as men who have sex with men (MSM). Phylogenetic analysis was conducted in 15 cases and revealed two distinct sequences of genotype IA linking to clusters recognised in MSM in other European countries in 2016; genotype IB was recognized in only 2 cases. The report confi...
Source: New Microbiologica - June 5, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

Genomic Characterization of an ST1153 PVL-producing Methicillin Resistant Staphylococcus aureus Clinical Isolate in Italy.
Authors: Rimoldi SG, Comandatore F, Longhi E, Romeri F, Piazza A, Pagani C, Tamoni A, Longobardi C, Negri C, Bestetti G, Gervasoni C, Perini M, Antinori S, Bandi C, Gismondo MR Abstract Methicillin-resistant Staphylococcus aureus (MRSA) clones are rapidly increasing beyond the hospital into the community, livestock farming and environmental settings (Lepuschitz S et al. 2018). An Italian man, a professional diver working in Egypt, was admitted to Infectious Diseases Clinic- ASST Fatebenefratelli Sacco for ulcerative skin lesions. An MRSA strain was isolated from the lesions' purulent exudate and the nasal colonizat...
Source: New Microbiologica - May 1, 2019 Category: Microbiology Tags: New Microbiol Source Type: research

CTX-M-15-producing uropathogenic Escherichia coli isolates at Rio de Janeiro, Brazil: Molecular epidemiology and MALDI-TOF MS.
Authors: Pavoni Gomes Chagas T, da Silva Caldeira LF, Vidal Pedinotti Zuma A, Cassiano de Oliveira I, D'Alincourt Carvalho-Assef AP, Dutra Asensi M, Piffano Costa Pellegrino FL Abstract Because of the high prevalence of CTX-M-15-producing Escherichia coli isolates causing urinary tract infections in Rio de Janeiro, we have investigated bla-CTX-M-15 gene presence, as well as CTXM- 15 production, in 32 E. coli isolates recovered from the urine of outpatients assisted at a public hospital located in the west zone of Rio. Molecular epidemiology was assessed by PFGE and phylotyping methods. The work highlights the good ...
Source: New Microbiologica - May 1, 2019 Category: Microbiology Tags: New Microbiol Source Type: research