Lamin B1 curtails early human papillomavirus infection by safeguarding nuclear compartmentalization and autophagic capacity
Cell Mol Life Sci. 2024 Mar 14;81(1):141. doi: 10.1007/s00018-024-05194-3.ABSTRACTHuman papillomavirus (HPV) infection is a primary cause of cervical and head-and-neck cancers. The HPV genome enters the nucleus during mitosis when the nuclear envelope disassembles. Given that lamins maintain nuclear integrity during interphase, we asked to what extent their loss would affect early HPV infection. To address this question, we infected human cervical cancer cells and keratinocytes lacking the major lamins with a HPV16 pseudovirus (HP-PsV) encoding an EGFP reporter. We found that a sustained reduction or complete loss of lamin...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Freya Molenberghs Marlies Verschuuren Lauran Vandeweyer Sarah Peeters Johannes J Bogers Claudina Perez Novo Wim Vanden Berghe Hans De Reu Nathalie Cools Mario Schelhaas Winnok H De Vos Source Type: research

Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation
Cell Mol Life Sci. 2024 Mar 15;81(1):142. doi: 10.1007/s00018-024-05161-y.ABSTRACTThioredoxin interacting protein (Txnip) is a stress-responsive factor regulating Trx1 for redox balance and involved in diverse cellular processes including proliferation, differentiation, apoptosis, inflammation, and metabolism. However, the biological role of Txnip function in stem cell pluripotency has yet to be investigated. Here, we reveal the novel functions of mouse Txnip in cellular reprogramming and differentiation onset by involving in glucose-mediated histone acetylation and the regulation of Oct4, which is a fundamental component ...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Sojung Kwak Cho Lok Song Yee Sook Cho Inpyo Choi Jae-Eun Byun Haiyoung Jung Jungwoon Lee Source Type: research

High-content method for mechanosignaling studies using IsoStretcher technology and quantitative Ca < sup > 2+ < /sup > imaging applied to Piezo1 in cardiac HL-1 cells
Cell Mol Life Sci. 2024 Mar 14;81(1):140. doi: 10.1007/s00018-024-05159-6.ABSTRACTThe importance of mechanosensory transduction pathways in cellular signalling has prominently come to focus in the last decade with the discovery of the Piezo ion channel family. Mechanosignaling involving Piezo1 ion channels in the function of the heart and cardiovascular system has only recently been identified to have implications for cardiovascular physiology and pathophysiology, in particular for heart failure (i.e., hypertrophy or dilative cardiomyopathy). These results have emphasized the need for higher throughput methods to study sin...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Anna-Lena Merten Ulrike Sch öler Yang Guo Fabian Linsenmeier Boris Martinac Oliver Friedrich Sebastian Sch ürmann Source Type: research

Lamin B1 curtails early human papillomavirus infection by safeguarding nuclear compartmentalization and autophagic capacity
Cell Mol Life Sci. 2024 Mar 14;81(1):141. doi: 10.1007/s00018-024-05194-3.ABSTRACTHuman papillomavirus (HPV) infection is a primary cause of cervical and head-and-neck cancers. The HPV genome enters the nucleus during mitosis when the nuclear envelope disassembles. Given that lamins maintain nuclear integrity during interphase, we asked to what extent their loss would affect early HPV infection. To address this question, we infected human cervical cancer cells and keratinocytes lacking the major lamins with a HPV16 pseudovirus (HP-PsV) encoding an EGFP reporter. We found that a sustained reduction or complete loss of lamin...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Freya Molenberghs Marlies Verschuuren Lauran Vandeweyer Sarah Peeters Johannes J Bogers Claudina Perez Novo Wim Vanden Berghe Hans De Reu Nathalie Cools Mario Schelhaas Winnok H De Vos Source Type: research

Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation
Cell Mol Life Sci. 2024 Mar 15;81(1):142. doi: 10.1007/s00018-024-05161-y.ABSTRACTThioredoxin interacting protein (Txnip) is a stress-responsive factor regulating Trx1 for redox balance and involved in diverse cellular processes including proliferation, differentiation, apoptosis, inflammation, and metabolism. However, the biological role of Txnip function in stem cell pluripotency has yet to be investigated. Here, we reveal the novel functions of mouse Txnip in cellular reprogramming and differentiation onset by involving in glucose-mediated histone acetylation and the regulation of Oct4, which is a fundamental component ...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Sojung Kwak Cho Lok Song Yee Sook Cho Inpyo Choi Jae-Eun Byun Haiyoung Jung Jungwoon Lee Source Type: research

High-content method for mechanosignaling studies using IsoStretcher technology and quantitative Ca < sup > 2+ < /sup > imaging applied to Piezo1 in cardiac HL-1 cells
Cell Mol Life Sci. 2024 Mar 14;81(1):140. doi: 10.1007/s00018-024-05159-6.ABSTRACTThe importance of mechanosensory transduction pathways in cellular signalling has prominently come to focus in the last decade with the discovery of the Piezo ion channel family. Mechanosignaling involving Piezo1 ion channels in the function of the heart and cardiovascular system has only recently been identified to have implications for cardiovascular physiology and pathophysiology, in particular for heart failure (i.e., hypertrophy or dilative cardiomyopathy). These results have emphasized the need for higher throughput methods to study sin...
Source: Cellular and Molecular Life Sciences : CMLS - March 15, 2024 Category: Cytology Authors: Anna-Lena Merten Ulrike Sch öler Yang Guo Fabian Linsenmeier Boris Martinac Oliver Friedrich Sebastian Sch ürmann Source Type: research

A novel mouse model for N-terminal truncated A β2-x generation through meprin β overexpression in astrocytes
Cell Mol Life Sci. 2024 Mar 13;81(1):139. doi: 10.1007/s00018-024-05139-w.ABSTRACTNeurotoxic amyloid-β (Aβ) peptides cause neurodegeneration in Alzheimer's disease (AD) patients' brains. They are released upon proteolytic processing of the amyloid precursor protein (APP) extracellularly at the β-secretase site and intramembranously at the γ-secretase site. Several AD mouse models were developed to conduct respective research in vivo. Most of these classical models overexpress human APP with mutations driving AD-associated pathogenic APP processing. However, the resulting pattern of Aβ species in the mouse brains diffe...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2024 Category: Cytology Authors: Fred Armbrust Kira Bickenbach Hermann Altmeppen Angelica Foggetti Anne Winkelmann Peer Wulff Markus Glatzel Claus U Pietrzik Christoph Becker-Pauly Source Type: research

A novel mouse model for N-terminal truncated A β2-x generation through meprin β overexpression in astrocytes
Cell Mol Life Sci. 2024 Mar 13;81(1):139. doi: 10.1007/s00018-024-05139-w.ABSTRACTNeurotoxic amyloid-β (Aβ) peptides cause neurodegeneration in Alzheimer's disease (AD) patients' brains. They are released upon proteolytic processing of the amyloid precursor protein (APP) extracellularly at the β-secretase site and intramembranously at the γ-secretase site. Several AD mouse models were developed to conduct respective research in vivo. Most of these classical models overexpress human APP with mutations driving AD-associated pathogenic APP processing. However, the resulting pattern of Aβ species in the mouse brains diffe...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2024 Category: Cytology Authors: Fred Armbrust Kira Bickenbach Hermann Altmeppen Angelica Foggetti Anne Winkelmann Peer Wulff Markus Glatzel Claus U Pietrzik Christoph Becker-Pauly Source Type: research

ALKBH1 promotes HIF-1 α-mediated glycolysis by inhibiting N-glycosylation of LAMP2A
In conclusion, we have revealed a non-demethylation role of ALKBH1 in regulating N-glycosylation of LAMPs by interacting with OST subunits and CMA-mediated degradation of HIF-1α.PMID:38472355 | DOI:10.1007/s00018-024-05152-z (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Yanyan Liu Mengmeng Li Miao Lin Xinjie Liu Haolin Guo Junyang Tan Liubing Hu Jianshuang Li Qinghua Zhou Source Type: research

Glymphatic-lymphatic coupling: assessment of the evidence from magnetic resonance imaging of humans
Cell Mol Life Sci. 2024 Mar 13;81(1):131. doi: 10.1007/s00018-024-05141-2.ABSTRACTThe discoveries that cerebrospinal fluid participates in metabolic perivascular exchange with the brain and further drains solutes to meningeal lymphatic vessels have sparked a tremendous interest in translating these seminal findings from animals to humans. A potential two-way coupling between the brain extra-vascular compartment and the peripheral immune system has implications that exceed those concerning neurodegenerative diseases, but also imply that the central nervous system has pushed its immunological borders toward the periphery, wh...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Geir Ringstad Per Kristian Eide Source Type: research

Crosstalk between purinergic receptor P2Y < sub > 11 < /sub > and chemokine receptor CXCR7 is regulated by CXCR4 in human macrophages
Cell Mol Life Sci. 2024 Mar 13;81(1):132. doi: 10.1007/s00018-024-05158-7.ABSTRACTP2Y11 is a G protein-coupled ATP receptor that activates IL-1 receptor (IL-1R) in a cyclic AMP dependent manner. In human macrophages, P2Y11/IL-1R crosstalk with CCL20 as a prime target is controlled by phosphodiesterase 4 (PDE4), which mediates breakdown of cyclic AMP. Here, we used gene expression analysis to identify activation of CXCR4 and CXCR7 as a hallmark of P2Y11 signaling. We found that PDE4 inhibition with rolipram boosts P2Y11/IL-1R-induced upregulation of CXCR7 expression and CCL20 production in an epidermal growth factor recepto...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Dominik Klaver Hubert Gander Beatrice Frena Marco Amato Martin Thurnher Source Type: research

Inhibition of EHMT1/2 rescues synaptic damage and motor impairment in a PD mouse model
Cell Mol Life Sci. 2024 Mar 12;81(1):128. doi: 10.1007/s00018-024-05176-5.ABSTRACTEpigenetic dysregulation that leads to alterations in gene expression and is suggested to be one of the key pathophysiological factors of Parkinson's disease (PD). Here, we found that α-synuclein preformed fibrils (PFFs) induced histone H3 dimethylation at lysine 9 (H3K9me2) and increased the euchromatic histone methyltransferases EHMT1 and EHMT2, which were accompanied by neuronal synaptic damage, including loss of synapses and diminished expression levels of synaptic-related proteins. Furthermore, the levels of H3K9me2 at promoters in gene...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Zhixiong Zhang Rui Wang Hui Zhou Dan Wu Yifan Cao Chuang Zhang Hongyang Sun Chenchen Mu Zongbing Hao Haigang Ren Nana Wang Shuang Yu Jingzhong Zhang Mengdan Tao Can Wang Yan Liu Liu Liu Yanli Liu Jianye Zang Guanghui Wang Source Type: research

Unraveling the molecular interactions between α7 nicotinic receptor and a RIC3 variant associated with backward speech
Cell Mol Life Sci. 2024 Mar 12;81(1):129. doi: 10.1007/s00018-024-05149-8.ABSTRACTRecent work putatively linked a rare genetic variant of the chaperone Resistant to Inhibitors of acetylcholinesterase (RIC3) (NM_024557.4:c.262G > A, NP_078833.3:p.G88R) to a unique ability to speak backwards, a language skill that is associated with exceptional working memory capacity. RIC3 is important for the folding, maturation, and functional expression of α7 nicotinic acetylcholine receptors (nAChR). We compared and contrasted the effects of RIC3G88R on assembly, cell surface expression, and function of human α7 receptors using flu...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Aditi Pradhan Hayley Mounford Jessica Peixinho Edward Rea Emmanouela Epeslidou Julia S Scott Joanna Cull Susan Maxwell Richard Webster David Beeson Yin Yao Dong Stefan Prekovic Isabel Bermudez Dianne F Newbury Source Type: research

Lipid homeostasis is essential for oogenesis and embryogenesis in the silkworm, Bombyx mori
Cell Mol Life Sci. 2024 Mar 12;81(1):127. doi: 10.1007/s00018-024-05173-8.ABSTRACTReproduction, a fundamental feature of all known life, closely correlates with energy homeostasis. The control of synthesizing and mobilizing lipids are dynamic and well-organized processes to distribute lipid resources across tissues or generations. However, how lipid homeostasis is precisely coordinated during insect reproductive development is poorly understood. Here we describe the relations between energy metabolism and reproduction in the silkworm, Bombyx mori, a lepidopteran model insect, by using CRISPR/Cas9-mediated mutation analysis...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Fangying Yang Xiaoyan Xu Bo Hu Zhongjie Zhang Kai Chen Ye Yu Hua Bai Anjiang Tan Source Type: research

PEBP4 deficiency aggravates LPS-induced acute lung injury and alveolar fluid clearance impairment via modulating PI3K/AKT signaling pathway
In this study, we generated PEBP4 knockout (KO) mice to investigate the potential function of PEBP4, as well as to evaluate the capacity of alveolar fluid clearance (AFC) and the activity of phosphatidylinositide 3-kinases (PI3K)/serine-theronine protein kinase B (PKB, also known as AKT) signaling pathway in lipopolysaccharide (LPS)-induced ALI mice models. We found that PEBP4 deficiency exacerbated lung pathological damage and edema, and increased the wet/dry weight ratio and total protein concentration of bronchoalveolar lavage fluid (BALF) in LPS-treated mice. Meanwhile, PEBP4 KO promoted an LPS-induced rise in the pulm...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2024 Category: Cytology Authors: Qiao-Qing Shi Yong-Hong Huang Yu-Fei Li Shuang-Yan Zhen Yan-Hong Li Jia-Yi Huang Jia-Yang Wang Xiao-Yan Zhou Source Type: research