Cellular and Molecular Life Sciences : CMLS This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

PALB2-mutated human mammary cells display a broad spectrum of morphological and functional abnormalities induced by increased TGF β signaling
Cell Mol Life Sci. 2024 Apr 10;81(1):173. doi: 10.1007/s00018-024-05183-6.ABSTRACTHeterozygous mutations in any of three major genes, BRCA1, BRCA2 and PALB2, are associated with high-risk hereditary breast cancer susceptibility frequently seen as familial disease clustering. PALB2 is a key interaction partner and regulator of several vital cellular activities of BRCA1 and BRCA2, and is thus required for DNA damage repair and alleviation of replicative and oxidative stress. Little is however known about how PALB2-deficiency affects cell function beyond that, especially in the three-dimensional setting, and also about its ro...
Source: Cellular and Molecular Life Sciences : CMLS - April 10, 2024 Category: Cytology Authors: Hanna Tuppurainen Niina Laurila Marjut N ätynki Leila Eshraghi Anna Tervasm äki Louisa Erichsen Claus Storgaard S ørensen Katri Pylk äs Robert Winqvist Hellevi Peltoketo Source Type: research

Bioengineered MSC < sup > Cxcr2 < /sup > transdifferentiated keratinocyte-like cell-derived organoid potentiates skin regeneration through ERK1/2 and STAT3 signaling in diabetic wound
Cell Mol Life Sci. 2024 Apr 10;81(1):172. doi: 10.1007/s00018-023-05057-3.ABSTRACTSkin regeneration is severely compromised in diabetic foot ulcers. Allogeneic mesenchymal stem cell (MSC) transplantation is limited due to the poor engraftment, mitogenic, and differentiation potential in the harsh wound microenvironment. Thus, to improve the efficacy of cell therapy, the chemokine receptor Cxcr2 was overexpressed in MSCs (MSCCxcr2). CXCL2/CXCR2 axis induction led to the enhanced proliferation of MSCs through the activation of STAT3 and ERK1/2 signaling. Transcriptional upregulation of FGFR2IIIb (KGF Receptor) promoter by th...
Source: Cellular and Molecular Life Sciences : CMLS - April 10, 2024 Category: Cytology Authors: Subholakshmi Choudhury Neha R Dhoke Shilpa Chawla Amitava Das Source Type: research

Metabolic profiling identifies Qrich2 as a novel glutamine sensor that regulates microtubule glutamylation and mitochondrial function in mouse sperm
Cell Mol Life Sci. 2024 Apr 10;81(1):170. doi: 10.1007/s00018-024-05177-4.ABSTRACTIn our prior investigation, we discerned loss-of-function variants within the gene encoding glutamine-rich protein 2 (QRICH2) in two consanguineous families, leading to various morphological abnormalities in sperm flagella and male infertility. The Qrich2 knockout (KO) in mice also exhibits multiple morphological abnormalities of the flagella (MMAF) phenotype with a significantly decreased sperm motility. However, how ORICH2 regulates the formation of sperm flagella remains unclear. Abnormal glutamylation levels of tubulin cause dysplastic mi...
Source: Cellular and Molecular Life Sciences : CMLS - April 10, 2024 Category: Cytology Authors: Guohui Zhang Juncen Guo Haoxuan Yang Qing Li Fei Ye Yuelin Song Dongsheng Xiong Jiuzhi Zeng Weiwei Zhi Shuiqiao Yuan Yunyun Lv Tongtong Li Yan Wang Lu Liao Dong Deng Weixin Liu Wenming Xu Source Type: research

The E3 ubiquitin ligase ITCH negatively regulates intercellular communication via gap junctions by targeting connexin43 for lysosomal degradation
Cell Mol Life Sci. 2024 Apr 10;81(1):171. doi: 10.1007/s00018-024-05165-8.ABSTRACTIntercellular communication via gap junctions has a fundamental role in regulating cell growth and tissue homeostasis, and its dysregulation may be involved in cancer development and radio- and chemotherapy resistance. Connexin43 (Cx43) is the most ubiquitously expressed gap junction channel protein in human tissues. Emerging evidence indicates that dysregulation of the sorting of Cx43 to lysosomes is important in mediating the loss of Cx43-based gap junctions in cancer cells. However, the molecular basis underlying this process is currently ...
Source: Cellular and Molecular Life Sciences : CMLS - April 10, 2024 Category: Cytology Authors: Max Zachrisson Totland Lars M ørland Knudsen Nikoline Lander Rasmussen Yasufumi Omori Vigdis S ørensen Vilde C Wivestad Elster Jakob M ørkved Stenersen Mathias Larsen Caroline Lunder Jensen Anna A Zickfeldt Lade Emilie Bruusgaard Sebastian Basing Kusht Source Type: research

A novel fatty acid analogue triggers CD36-GPR120 interaction and exerts anti-inflammatory action in endotoxemia
Cell Mol Life Sci. 2024 Apr 10;81(1):176. doi: 10.1007/s00018-024-05207-1.ABSTRACTInflammation is a mediator of a number of chronic pathologies. We synthesized the diethyl (9Z,12Z)-octadeca-9,12-dien-1-ylphosphonate, called NKS3, which decreased lipopolysaccharide (LPS)-induced mRNA upregulation of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) not only in primary intraperitoneal and lung alveolar macrophages, but also in freshly isolated mice lung slices. The in-silico studies suggested that NKS3, being CD36 agonist, will bind to GPR120. Co-immunoprecipitation and proximity ligation assays demonstrated that NKS3 indu...
Source: Cellular and Molecular Life Sciences : CMLS - April 10, 2024 Category: Cytology Authors: Pierre-Marie Boutanquoi Amira Sayed Khan Lidia Cabeza Lucas Jantzen Thomas Gautier Semen Yesylevskyy Christophe Ramseyer David Masson Vincent Van Waes Aziz Hichami Naim Akhtar Khan Source Type: research

Kinesin KIF3A regulates meiotic progression and spindle assembly in oocyte meiosis
In this study, we characterized the critical roles of KIF3A during mouse oocyte meiosis. We found that KIF3A associated with microtubules during meiosis and depletion of KIF3A resulted in oocyte maturation defects. LC-MS data indicated that KIF3A associated with cell cycle regulation, cytoskeleton, mitochondrial function and intracellular transport-related molecules. Depletion of KIF3A activated the spindle assembly checkpoint, leading to metaphase I arrest of the first meiosis. In addition, KIF3A depletion caused aberrant spindle pole organization based on its association with KIFC1 to regulate expression and polar locali...
Source: Cellular and Molecular Life Sciences : CMLS - April 8, 2024 Category: Cytology Authors: Jing-Cai Liu Zhen-Nan Pan Jia-Qian Ju Yuan-Jing Zou Meng-Hao Pan Yue Wang Xin Wu Shao-Chen Sun Source Type: research

Rhes, a striatal enriched protein, regulates post-translational small-ubiquitin-like-modifier (SUMO) modification of nuclear proteins and alters gene expression
Cell Mol Life Sci. 2024 Apr 8;81(1):169. doi: 10.1007/s00018-024-05181-8.ABSTRACTRhes (Ras homolog enriched in the striatum), a multifunctional protein that regulates striatal functions associated with motor behaviors and neurological diseases, can shuttle from cell to cell via the formation of tunneling-like nanotubes (TNTs). However, the mechanisms by which Rhes mediates diverse functions remain unclear. Rhes is a small GTPase family member which contains a unique C-terminal Small Ubiquitin-like Modifier (SUMO) E3-like domain that promotes SUMO post-translational modification of proteins (SUMOylation) by promoting "cross...
Source: Cellular and Molecular Life Sciences : CMLS - April 8, 2024 Category: Cytology Authors: Oscar Rivera Manish Sharma Sunayana Dagar Neelam Shahani Uri Nimrod Ram ĺrez-Jarquĺn Gogce Crynen Pabalu Karunadharma Francis McManus Eric Bonneil Thibault Pierre Srinivasa Subramaniam Source Type: research

NFKBIZ regulates NF κB signaling pathway to mediate tumorigenesis and metastasis of hepatocellular carcinoma by direct interaction with TRIM16
In conclusion, our study demonstrated that NFKBIZ regulated HCC tumorigenesis and metastasis by mediating NFκB signal transduction and TRIM16/NFKBIZ/NFκB axis may be the underlying mechanism of sorafenib insensitivity in HCC.PMID:38581570 | PMC:PMC10998794 | DOI:10.1007/s00018-024-05182-7 (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - April 6, 2024 Category: Cytology Authors: Danfeng Guo Ming Zhang Tingju Wei Xiaodan Zhang Xiaoyi Shi Hongwei Tang Mingjie Ding Jie Li Shuijun Zhang Wenzhi Guo Source Type: research

Unearthing FLVCR1a: tracing the path to a vital cellular transporter
Cell Mol Life Sci. 2024 Apr 6;81(1):166. doi: 10.1007/s00018-024-05205-3.ABSTRACTThe Feline Leukemia Virus Subgroup C Receptor 1a (FLVCR1a) is a member of the SLC49 Major Facilitator Superfamily of transporters. Initially recognized as the receptor for the retrovirus responsible of pure red cell aplasia in cats, nearly two decades since its discovery, FLVCR1a remains a puzzling transporter, with ongoing discussions regarding what it transports and how its expression is regulated. Nonetheless, despite this, the substantial body of evidence accumulated over the years has provided insights into several critical processes in w...
Source: Cellular and Molecular Life Sciences : CMLS - April 6, 2024 Category: Cytology Authors: Veronica Fiorito Emanuela Tolosano Source Type: research

NFKBIZ regulates NF κB signaling pathway to mediate tumorigenesis and metastasis of hepatocellular carcinoma by direct interaction with TRIM16
In conclusion, our study demonstrated that NFKBIZ regulated HCC tumorigenesis and metastasis by mediating NFκB signal transduction and TRIM16/NFKBIZ/NFκB axis may be the underlying mechanism of sorafenib insensitivity in HCC.PMID:38581570 | DOI:10.1007/s00018-024-05182-7 (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - April 6, 2024 Category: Cytology Authors: Danfeng Guo Ming Zhang Tingju Wei Xiaodan Zhang Xiaoyi Shi Hongwei Tang Mingjie Ding Jie Li Shuijun Zhang Wenzhi Guo Source Type: research

CRL4 < sup > DCAF13 < /sup > E3 ubiquitin ligase targets MeCP2 for degradation to prevent DNA hypermethylation and ensure normal transcription in growing oocytes
In this study, we found MeCP2 protein was highly expressed in primordial and primary follicle, but was almost undetectable in secondary follicles. However, in aged ovary, MeCP2 protein is significantly increased in both oocyte and granulosa cells. Overexpression of MeCP2 in growing oocyte caused transcription dysregulation, DNA hypermethylation, and genome instability, ultimately leading to follicle growth arrest and apoptosis. MeCP2 is targeted by DCAF13, a substrate recognition adaptor of the Cullin 4-RING (CRL4) E3 ligase, and polyubiquitinated for degradation in both cells and oocytes. Dcaf13-null oocyte exhibited an a...
Source: Cellular and Molecular Life Sciences : CMLS - April 5, 2024 Category: Cytology Authors: Peipei Ren Xiaomei Tong Junjian Li Huifang Jiang Siya Liu Xiang Li Mengru Lai Weijie Yang Yan Rong Yingyi Zhang Jiamin Jin Yerong Ma Weiwei Pan Heng-Yu Fan Songying Zhang Yin-Li Zhang Source Type: research