Contribution of cell death signaling to blood vessel formation
Cell Mol Life Sci. 2021 Mar 30. doi: 10.1007/s00018-020-03738-x. Online ahead of print.ABSTRACTThe formation of new blood vessels is driven by proliferation of endothelial cells (ECs), elongation of maturing vessel sprouts and ultimately vessel remodeling to create a hierarchically structured vascular system. Vessel regression is an essential process to remove redundant vessel branches in order to adapt the final vessel density to the demands of the surrounding tissue. How exactly vessel regression occurs and whether and to which extent cell death contributes to this process has been in the focus of several studies within ...
Source: Cellular and Molecular Life Sciences : CMLS - March 30, 2021 Category: Cytology Authors: Nathalie Tisch Carmen Ruiz de Almod óvar Source Type: research

Kindlin3 regulates biophysical properties and mechanics of membrane to cortex attachment
Cell Mol Life Sci. 2021 Mar 30. doi: 10.1007/s00018-021-03817-7. Online ahead of print.ABSTRACTKindlin3 (K3), a FERM domain containing protein expressed in hematopoietic cells controls integrin activation and thus hemostatic and inflammatory responses. However, its role in the mechanics of plasma membrane remains unclear. Here, we show that genetic knockout of K3 in microglia and macrophages resulted in defective plasma membrane tension and membrane blebbing. Atomic force microscopy (AFM) of K3-deficient cells revealed a significant loss in membrane-to-cortex attachment (MCA), and consequently reduced membrane tension. Thi...
Source: Cellular and Molecular Life Sciences : CMLS - March 30, 2021 Category: Cytology Authors: Tejasvi Dudiki Gautam Mahajan Huan Liu Irina Zhevlakova Chase Bertagnolli Daniel W Nascimento Chandrasekhar R Kothapalli Tatiana V Byzova Source Type: research

Correction to: The role of connexin proteins and their channels in radiation-induced atherosclerosis
Cell Mol Life Sci. 2021 Mar 29. doi: 10.1007/s00018-021-03811-z. Online ahead of print.NO ABSTRACTPMID:33778901 | DOI:10.1007/s00018-021-03811-z (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - March 29, 2021 Category: Cytology Authors: Raghda Ramadan Sarah Baatout An Aerts Luc Leybaert Source Type: research

Correction to: The role of connexin proteins and their channels in radiation-induced atherosclerosis
Cell Mol Life Sci. 2021 Mar 29. doi: 10.1007/s00018-021-03811-z. Online ahead of print.NO ABSTRACTPMID:33778901 | DOI:10.1007/s00018-021-03811-z (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - March 29, 2021 Category: Cytology Authors: Raghda Ramadan Sarah Baatout An Aerts Luc Leybaert Source Type: research

The enteric nervous system in gastrointestinal disease etiology
Cell Mol Life Sci. 2021 Mar 26. doi: 10.1007/s00018-021-03812-y. Online ahead of print.ABSTRACTA highly conserved but convoluted network of neurons and glial cells, the enteric nervous system (ENS), is positioned along the wall of the gut to coordinate digestive processes and gastrointestinal homeostasis. Because ENS components are in charge of the autonomous regulation of gut function, it is inevitable that their dysfunction is central to the pathophysiology and symptom generation of gastrointestinal disease. While for neurodevelopmental disorders such as Hirschsprung, ENS pathogenesis appears to be clear-cut, the role fo...
Source: Cellular and Molecular Life Sciences : CMLS - March 26, 2021 Category: Cytology Authors: Amy Marie Holland Ana Carina Bon-Frauches Daniel Keszthelyi Veerle Melotte Werend Boesmans Source Type: research

The enteric nervous system in gastrointestinal disease etiology
Cell Mol Life Sci. 2021 Mar 26. doi: 10.1007/s00018-021-03812-y. Online ahead of print.ABSTRACTA highly conserved but convoluted network of neurons and glial cells, the enteric nervous system (ENS), is positioned along the wall of the gut to coordinate digestive processes and gastrointestinal homeostasis. Because ENS components are in charge of the autonomous regulation of gut function, it is inevitable that their dysfunction is central to the pathophysiology and symptom generation of gastrointestinal disease. While for neurodevelopmental disorders such as Hirschsprung, ENS pathogenesis appears to be clear-cut, the role fo...
Source: Cellular and Molecular Life Sciences : CMLS - March 26, 2021 Category: Cytology Authors: Amy Marie Holland Ana Carina Bon-Frauches Daniel Keszthelyi Veerle Melotte Werend Boesmans Source Type: research

The enteric nervous system in gastrointestinal disease etiology
Cell Mol Life Sci. 2021 Mar 26. doi: 10.1007/s00018-021-03812-y. Online ahead of print.ABSTRACTA highly conserved but convoluted network of neurons and glial cells, the enteric nervous system (ENS), is positioned along the wall of the gut to coordinate digestive processes and gastrointestinal homeostasis. Because ENS components are in charge of the autonomous regulation of gut function, it is inevitable that their dysfunction is central to the pathophysiology and symptom generation of gastrointestinal disease. While for neurodevelopmental disorders such as Hirschsprung, ENS pathogenesis appears to be clear-cut, the role fo...
Source: Cellular and Molecular Life Sciences : CMLS - March 26, 2021 Category: Cytology Authors: Amy Marie Holland Ana Carina Bon-Frauches Daniel Keszthelyi Veerle Melotte Werend Boesmans Source Type: research

Enzymology of extracellular NAD metabolism
Cell Mol Life Sci. 2021 Mar 23. doi: 10.1007/s00018-020-03742-1. Online ahead of print.ABSTRACTExtracellular NAD represents a key signaling molecule in different physiological and pathological conditions. It exerts such function both directly, through the activation of specific purinergic receptors, or indirectly, serving as substrate of ectoenzymes, such as CD73, nucleotide pyrophosphatase/phosphodiesterase 1, CD38 and its paralog CD157, and ecto ADP ribosyltransferases. By hydrolyzing NAD, these enzymes dictate extracellular NAD availability, thus regulating its direct signaling role. In addition, they can generate from ...
Source: Cellular and Molecular Life Sciences : CMLS - March 23, 2021 Category: Cytology Authors: Massimiliano Gasparrini Leonardo Sorci Nadia Raffaelli Source Type: research

Enzymology of extracellular NAD metabolism
Cell Mol Life Sci. 2021 Mar 23. doi: 10.1007/s00018-020-03742-1. Online ahead of print.ABSTRACTExtracellular NAD represents a key signaling molecule in different physiological and pathological conditions. It exerts such function both directly, through the activation of specific purinergic receptors, or indirectly, serving as substrate of ectoenzymes, such as CD73, nucleotide pyrophosphatase/phosphodiesterase 1, CD38 and its paralog CD157, and ecto ADP ribosyltransferases. By hydrolyzing NAD, these enzymes dictate extracellular NAD availability, thus regulating its direct signaling role. In addition, they can generate from ...
Source: Cellular and Molecular Life Sciences : CMLS - March 23, 2021 Category: Cytology Authors: Massimiliano Gasparrini Leonardo Sorci Nadia Raffaelli Source Type: research

Enzymology of extracellular NAD metabolism
Cell Mol Life Sci. 2021 Mar 23. doi: 10.1007/s00018-020-03742-1. Online ahead of print.ABSTRACTExtracellular NAD represents a key signaling molecule in different physiological and pathological conditions. It exerts such function both directly, through the activation of specific purinergic receptors, or indirectly, serving as substrate of ectoenzymes, such as CD73, nucleotide pyrophosphatase/phosphodiesterase 1, CD38 and its paralog CD157, and ecto ADP ribosyltransferases. By hydrolyzing NAD, these enzymes dictate extracellular NAD availability, thus regulating its direct signaling role. In addition, they can generate from ...
Source: Cellular and Molecular Life Sciences : CMLS - March 23, 2021 Category: Cytology Authors: Massimiliano Gasparrini Leonardo Sorci Nadia Raffaelli Source Type: research

Nonsense suppression therapies in human genetic diseases
Cell Mol Life Sci. 2021 Mar 22. doi: 10.1007/s00018-021-03809-7. Online ahead of print.ABSTRACTAbout 11% of all human disease-associated gene lesions are nonsense mutations, resulting in the introduction of an in-frame premature translation-termination codon (PTC) into the protein-coding gene sequence. When translated, PTC-containing mRNAs originate truncated and often dysfunctional proteins that might be non-functional or have gain-of-function or dominant-negative effects. Therapeutic strategies aimed at suppressing PTCs to restore deficient protein function-the so-called nonsense suppression (or PTC readthrough) therapie...
Source: Cellular and Molecular Life Sciences : CMLS - March 22, 2021 Category: Cytology Authors: Patr ícia Martins-Dias Lu ísa Romão Source Type: research

Mitochondrial network remodeling: an important feature of myogenesis and skeletal muscle regeneration
Cell Mol Life Sci. 2021 Mar 22. doi: 10.1007/s00018-021-03807-9. Online ahead of print.ABSTRACTThe remodeling of the mitochondrial network is a critical process in maintaining cellular homeostasis and is intimately related to mitochondrial function. The interplay between the formation of new mitochondria (biogenesis) and the removal of damaged mitochondria (mitophagy) provide a means for the repopulation of the mitochondrial network. Additionally, mitochondrial fission and fusion serve as a bridge between biogenesis and mitophagy. In recent years, the importance of these processes has been characterised in multiple tissue-...
Source: Cellular and Molecular Life Sciences : CMLS - March 22, 2021 Category: Cytology Authors: Fasih Ahmad Rahman Joe Quadrilatero Source Type: research

The complement system in age-related macular degeneration
Cell Mol Life Sci. 2021 Mar 9. doi: 10.1007/s00018-021-03796-9. Online ahead of print.ABSTRACTAge-related macular degeneration (AMD) is a chronic and progressive degenerative disease of the retina, which culminates in blindness and affects mainly the elderly population. AMD pathogenesis and pathophysiology are incredibly complex due to the structural and cellular complexity of the retina, and the variety of risk factors and molecular mechanisms that contribute to disease onset and progression. AMD is driven by a combination of genetic predisposition, natural ageing changes and lifestyle factors, such as smoking or nutritio...
Source: Cellular and Molecular Life Sciences : CMLS - March 22, 2021 Category: Cytology Authors: Angela Armento Marius Ueffing Simon J Clark Source Type: research

Oxidative stress and impaired oligodendrocyte precursor cell differentiation in neurological disorders
Cell Mol Life Sci. 2021 Mar 10. doi: 10.1007/s00018-021-03802-0. Online ahead of print.ABSTRACTOligodendrocyte precursor cells (OPCs) account for 5% of the resident parenchymal central nervous system glial cells. OPCs are not only a back-up for the loss of oligodendrocytes that occurs due to brain injury or inflammation-induced demyelination (remyelination) but are also pivotal in plastic processes such as learning and memory (adaptive myelination). OPC differentiation into mature myelinating oligodendrocytes is controlled by a complex transcriptional network and depends on high metabolic and mitochondrial demand. Mounting...
Source: Cellular and Molecular Life Sciences : CMLS - March 22, 2021 Category: Cytology Authors: Jan Spaas Lieve van Veggel Melissa Schepers Assia Tiane Jack van Horssen David M Wilson Pablo R Moya Elisabeth Piccart Niels Hellings Bert O Eijnde Wim Derave Rudy Schreiber Tim Vanmierlo Source Type: research

Transcriptional and epigenetic regulation of PD-1 expression
Cell Mol Life Sci. 2021 Mar 18. doi: 10.1007/s00018-020-03737-y. Online ahead of print.ABSTRACTProgrammed cell death-1 (PD-1) is a co-inhibitory receptor that plays important roles in regulating T cell immunity and peripheral tolerance. PD-1 signaling prevents T cells from overactivation during acute infections, but it maintains T cell exhaustion during chronic infections. Tumor cells can exploit the PD-1 signaling pathway to evade antitumor immune responses. The PD-1 signaling pathway is also essential for maintaining peripheral tolerance and prevention of autoimmunity. PD-1 expression is strictly and differentially regul...
Source: Cellular and Molecular Life Sciences : CMLS - March 19, 2021 Category: Cytology Authors: Zengde Chi Yan Lu Yinlong Yang Bingqing Li Peiyuan Lu Source Type: research

Transcriptional and epigenetic regulation of PD-1 expression
Cell Mol Life Sci. 2021 Mar 18. doi: 10.1007/s00018-020-03737-y. Online ahead of print.ABSTRACTProgrammed cell death-1 (PD-1) is a co-inhibitory receptor that plays important roles in regulating T cell immunity and peripheral tolerance. PD-1 signaling prevents T cells from overactivation during acute infections, but it maintains T cell exhaustion during chronic infections. Tumor cells can exploit the PD-1 signaling pathway to evade antitumor immune responses. The PD-1 signaling pathway is also essential for maintaining peripheral tolerance and prevention of autoimmunity. PD-1 expression is strictly and differentially regul...
Source: Cellular and Molecular Life Sciences : CMLS - March 19, 2021 Category: Cytology Authors: Zengde Chi Yan Lu Yinlong Yang Bingqing Li Peiyuan Lu Source Type: research

Transcriptional and epigenetic regulation of PD-1 expression
Cell Mol Life Sci. 2021 Mar 18. doi: 10.1007/s00018-020-03737-y. Online ahead of print.ABSTRACTProgrammed cell death-1 (PD-1) is a co-inhibitory receptor that plays important roles in regulating T cell immunity and peripheral tolerance. PD-1 signaling prevents T cells from overactivation during acute infections, but it maintains T cell exhaustion during chronic infections. Tumor cells can exploit the PD-1 signaling pathway to evade antitumor immune responses. The PD-1 signaling pathway is also essential for maintaining peripheral tolerance and prevention of autoimmunity. PD-1 expression is strictly and differentially regul...
Source: Cellular and Molecular Life Sciences : CMLS - March 19, 2021 Category: Cytology Authors: Zengde Chi Yan Lu Yinlong Yang Bingqing Li Peiyuan Lu Source Type: research

Transcriptional and epigenetic regulation of PD-1 expression
Cell Mol Life Sci. 2021 Mar 18. doi: 10.1007/s00018-020-03737-y. Online ahead of print.ABSTRACTProgrammed cell death-1 (PD-1) is a co-inhibitory receptor that plays important roles in regulating T cell immunity and peripheral tolerance. PD-1 signaling prevents T cells from overactivation during acute infections, but it maintains T cell exhaustion during chronic infections. Tumor cells can exploit the PD-1 signaling pathway to evade antitumor immune responses. The PD-1 signaling pathway is also essential for maintaining peripheral tolerance and prevention of autoimmunity. PD-1 expression is strictly and differentially regul...
Source: Cellular and Molecular Life Sciences : CMLS - March 19, 2021 Category: Cytology Authors: Zengde Chi Yan Lu Yinlong Yang Bingqing Li Peiyuan Lu Source Type: research

RNA and DNA G-quadruplexes bind to human dicer and inhibit its activity
Cell Mol Life Sci. 2021 Mar 17. doi: 10.1007/s00018-021-03795-w. Online ahead of print.ABSTRACTGuanine (G)-rich single-stranded nucleic acids can adopt G-quadruplex structures. Accumulating evidence indicates that G-quadruplexes serve important regulatory roles in fundamental biological processes such as DNA replication, transcription, and translation, while aberrant G-quadruplex formation is linked to genome instability and cancer. Understanding the biological functions played by G-quadruplexes requires detailed knowledge of their protein interactome. Here, we report that both RNA and DNA G-quadruplexes are bound by human...
Source: Cellular and Molecular Life Sciences : CMLS - March 18, 2021 Category: Cytology Authors: Natalia Koralewska Agnieszka Szczepanska Kinga Ciechanowska Marta Wojnicka Maria Pokornowska Marek C Milewski Dorota Gudanis Daniel Baranowski Chandran Nithin Janusz M Bujnicki Zofia Gdaniec Marek Figlerowicz Anna Kurzynska-Kokorniak Source Type: research

High dimensional profiling identifies specific immune types along the recovery trajectories of critically ill COVID19 patients
Cell Mol Life Sci. 2021 Mar 13:1-16. doi: 10.1007/s00018-021-03808-8. Online ahead of print.ABSTRACTThe COVID-19 pandemic poses a major burden on healthcare and economic systems across the globe. Even though a majority of the population develops only minor symptoms upon SARS-CoV-2 infection, a significant number are hospitalized at intensive care units (ICU) requiring critical care. While insights into the early stages of the disease are rapidly expanding, the dynamic immunological processes occurring in critically ill patients throughout their recovery at ICU are far less understood. Here, we have analysed whole blood sam...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2021 Category: Cytology Authors: P A Penttil ä S Van Gassen D Panovska L Vanderbeke Y Van Herck K Quintelier A Emmaneel J Filtjens B Malengier-Devlies K Ahmadzadeh P Van Mol D M Borr às A Antoranz F M Bosisio E Wauters K Martinod P Matthys Y Saeys A D Garg J Wauters F De Smet CONTAGIOU Source Type: research

High dimensional profiling identifies specific immune types along the recovery trajectories of critically ill COVID19 patients
Cell Mol Life Sci. 2021 Mar 13:1-16. doi: 10.1007/s00018-021-03808-8. Online ahead of print.ABSTRACTThe COVID-19 pandemic poses a major burden on healthcare and economic systems across the globe. Even though a majority of the population develops only minor symptoms upon SARS-CoV-2 infection, a significant number are hospitalized at intensive care units (ICU) requiring critical care. While insights into the early stages of the disease are rapidly expanding, the dynamic immunological processes occurring in critically ill patients throughout their recovery at ICU are far less understood. Here, we have analysed whole blood sam...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2021 Category: Cytology Authors: P A Penttil ä S Van Gassen D Panovska L Vanderbeke Y Van Herck K Quintelier A Emmaneel J Filtjens B Malengier-Devlies K Ahmadzadeh P Van Mol D M Borr às A Antoranz F M Bosisio E Wauters K Martinod P Matthys Y Saeys A D Garg J Wauters F De Smet CONTAGIOU Source Type: research

High dimensional profiling identifies specific immune types along the recovery trajectories of critically ill COVID19 patients
Cell Mol Life Sci. 2021 Mar 13. doi: 10.1007/s00018-021-03808-8. Online ahead of print.ABSTRACTThe COVID-19 pandemic poses a major burden on healthcare and economic systems across the globe. Even though a majority of the population develops only minor symptoms upon SARS-CoV-2 infection, a significant number are hospitalized at intensive care units (ICU) requiring critical care. While insights into the early stages of the disease are rapidly expanding, the dynamic immunological processes occurring in critically ill patients throughout their recovery at ICU are far less understood. Here, we have analysed whole blood samples ...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2021 Category: Cytology Authors: P A Penttil ä S Van Gassen D Panovska L Vanderbeke Y Van Herck K Quintelier A Emmaneel J Filtjens B Malengier-Devlies K Ahmadzadeh P Van Mol D M Borr às A Antoranz F M Bosisio E Wauters K Martinod P Matthys Y Saeys A D Garg J Wauters F De Smet CONTAGIOU Source Type: research

High dimensional profiling identifies specific immune types along the recovery trajectories of critically ill COVID19 patients
Cell Mol Life Sci. 2021 Mar 13. doi: 10.1007/s00018-021-03808-8. Online ahead of print.ABSTRACTThe COVID-19 pandemic poses a major burden on healthcare and economic systems across the globe. Even though a majority of the population develops only minor symptoms upon SARS-CoV-2 infection, a significant number are hospitalized at intensive care units (ICU) requiring critical care. While insights into the early stages of the disease are rapidly expanding, the dynamic immunological processes occurring in critically ill patients throughout their recovery at ICU are far less understood. Here, we have analysed whole blood samples ...
Source: Cellular and Molecular Life Sciences : CMLS - March 14, 2021 Category: Cytology Authors: P A Penttil ä S Van Gassen D Panovska L Vanderbeke Y Van Herck K Quintelier A Emmaneel J Filtjens B Malengier-Devlies K Ahmadzadeh P Van Mol D M Borr às A Antoranz F M Bosisio E Wauters K Martinod P Matthys Y Saeys A D Garg J Wauters F De Smet CONTAGIOU Source Type: research

Of membranes and malaria: phospholipid asymmetry in Plasmodium falciparum-infected red blood cells
Cell Mol Life Sci. 2021 Mar 13. doi: 10.1007/s00018-021-03799-6. Online ahead of print.ABSTRACTMalaria is a vector-borne parasitic disease with a vast impact on human history, and according to the World Health Organisation, Plasmodium parasites still infect over 200 million people per year. Plasmodium falciparum, the deadliest parasite species, has a remarkable ability to undermine the host immune system and cause life-threatening disease during blood infection. The parasite's host cells, red blood cells (RBCs), generally maintain an asymmetric distribution of phospholipids in the two leaflets of the plasma membrane bilaye...
Source: Cellular and Molecular Life Sciences : CMLS - March 13, 2021 Category: Cytology Authors: Merryn Fraser Kai Matuschewski Alexander G Maier Source Type: research

Multi-phaseted problems of TDP-43 in selective neuronal vulnerability in ALS
Cell Mol Life Sci. 2021 Mar 11. doi: 10.1007/s00018-021-03792-z. Online ahead of print.ABSTRACTTransactive response DNA-binding protein 43 kDa (TDP-43) encoded by the TARDBP gene is an evolutionarily conserved heterogeneous nuclear ribonucleoprotein (hnRNP) that regulates multiple steps of RNA metabolism, and its cytoplasmic aggregation characterizes degenerating motor neurons in amyotrophic lateral sclerosis (ALS). In most ALS cases, cytoplasmic TDP-43 aggregation occurs in the absence of mutations in the coding sequence of TARDBP. Thus, a major challenge in ALS research is to understand the nature of pathological changes...
Source: Cellular and Molecular Life Sciences : CMLS - March 12, 2021 Category: Cytology Authors: Kazuhide Asakawa Hiroshi Handa Koichi Kawakami Source Type: research

CMLS forum reviews: mitochondrial damage control
Cell Mol Life Sci. 2021 Mar 12. doi: 10.1007/s00018-021-03804-y. Online ahead of print.ABSTRACTMitochondria supply cellular energy through oxidative phosphorylation and fulfill numerous additional functions that are fundamental to cellular homeostasis and stress responses. Mitochondrial malfunction, arising from inherent defects of the organelle itself, aging, or acute or chronic stress, can cause substantial damage to organismal health. For instance, mitochondrial malfunction contributes to inflammation, neurodegeneration, tumorigenesis and cardiovascular diseases. Therefore, various quality control mechanisms exist that ...
Source: Cellular and Molecular Life Sciences : CMLS - March 12, 2021 Category: Cytology Authors: Anne Hamacher-Brady Source Type: research

Multi-phaseted problems of TDP-43 in selective neuronal vulnerability in ALS
Cell Mol Life Sci. 2021 Mar 11. doi: 10.1007/s00018-021-03792-z. Online ahead of print.ABSTRACTTransactive response DNA-binding protein 43 kDa (TDP-43) encoded by the TARDBP gene is an evolutionarily conserved heterogeneous nuclear ribonucleoprotein (hnRNP) that regulates multiple steps of RNA metabolism, and its cytoplasmic aggregation characterizes degenerating motor neurons in amyotrophic lateral sclerosis (ALS). In most ALS cases, cytoplasmic TDP-43 aggregation occurs in the absence of mutations in the coding sequence of TARDBP. Thus, a major challenge in ALS research is to understand the nature of pathological changes...
Source: Cellular and Molecular Life Sciences : CMLS - March 12, 2021 Category: Cytology Authors: Kazuhide Asakawa Hiroshi Handa Koichi Kawakami Source Type: research

CMLS forum reviews: mitochondrial damage control
Cell Mol Life Sci. 2021 Mar 12. doi: 10.1007/s00018-021-03804-y. Online ahead of print.ABSTRACTMitochondria supply cellular energy through oxidative phosphorylation and fulfill numerous additional functions that are fundamental to cellular homeostasis and stress responses. Mitochondrial malfunction, arising from inherent defects of the organelle itself, aging, or acute or chronic stress, can cause substantial damage to organismal health. For instance, mitochondrial malfunction contributes to inflammation, neurodegeneration, tumorigenesis and cardiovascular diseases. Therefore, various quality control mechanisms exist that ...
Source: Cellular and Molecular Life Sciences : CMLS - March 12, 2021 Category: Cytology Authors: Anne Hamacher-Brady Source Type: research

Multi-phaseted problems of TDP-43 in selective neuronal vulnerability in ALS
Cell Mol Life Sci. 2021 Mar 11. doi: 10.1007/s00018-021-03792-z. Online ahead of print.ABSTRACTTransactive response DNA-binding protein 43 kDa (TDP-43) encoded by the TARDBP gene is an evolutionarily conserved heterogeneous nuclear ribonucleoprotein (hnRNP) that regulates multiple steps of RNA metabolism, and its cytoplasmic aggregation characterizes degenerating motor neurons in amyotrophic lateral sclerosis (ALS). In most ALS cases, cytoplasmic TDP-43 aggregation occurs in the absence of mutations in the coding sequence of TARDBP. Thus, a major challenge in ALS research is to understand the nature of pathological changes...
Source: Cellular and Molecular Life Sciences : CMLS - March 12, 2021 Category: Cytology Authors: Kazuhide Asakawa Hiroshi Handa Koichi Kawakami Source Type: research

Cerebrovascular development: mechanisms and experimental approaches
Cell Mol Life Sci. 2021 Mar 10. doi: 10.1007/s00018-021-03790-1. Online ahead of print.ABSTRACTThe cerebral vasculature plays a central role in human health and disease and possesses several unique anatomic, functional and molecular characteristics. Despite their importance, the mechanisms that determine cerebrovascular development are less well studied than other vascular territories. This is in part due to limitations of existing models and techniques for visualisation and manipulation of the cerebral vasculature. In this review we summarise the experimental approaches used to study the cerebral vessels and the mechanism...
Source: Cellular and Molecular Life Sciences : CMLS - March 10, 2021 Category: Cytology Authors: Timothy J A Chico Elisabeth C Kugler Source Type: research

Cerebrovascular development: mechanisms and experimental approaches
Cell Mol Life Sci. 2021 Mar 10. doi: 10.1007/s00018-021-03790-1. Online ahead of print.ABSTRACTThe cerebral vasculature plays a central role in human health and disease and possesses several unique anatomic, functional and molecular characteristics. Despite their importance, the mechanisms that determine cerebrovascular development are less well studied than other vascular territories. This is in part due to limitations of existing models and techniques for visualisation and manipulation of the cerebral vasculature. In this review we summarise the experimental approaches used to study the cerebral vessels and the mechanism...
Source: Cellular and Molecular Life Sciences : CMLS - March 10, 2021 Category: Cytology Authors: Timothy J A Chico Elisabeth C Kugler Source Type: research

Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast
We describe here the optimisation of the α-factor preproleader to improve recombinant enzyme production in S. cerevisiae through two parallel engineering strategies: a bottom-up design over the native α-factor preproleader (αnat) and a top-down design over the fittest evolved signal peptide obtained in our lab (α9H2 leader). The goal was to analyse the effect of mutations accumulated in the signal sequence throughout iterations of directed evolution, or of other reported mutations, and their possible epistatic interactions. Both approaches agreed in the positive synergism of four mutations (Aα...
Source: Cellular and Molecular Life Sciences : CMLS - March 9, 2021 Category: Cytology Authors: Pablo Aza Gonzalo Molpeceres Felipe de Salas Susana Camarero Source Type: research

R-catcher, a potent molecular tool to unveil the arginylome
This study presents the first molecular tool that allows the unbiased identification of arginylated proteins, thereby unlocking the arginylome and provide a new path to disease biomarker discovery.PMID:33687501 | DOI:10.1007/s00018-021-03805-x (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - March 9, 2021 Category: Cytology Authors: Taewook Seo Jihyo Kim Ho-Chul Shin Jung Gi Kim Shinyeong Ju Laxman Nawale Goeun Han Hye Seon Lee Geul Bang Jin Young Kim Jeong Kyu Bang Kyung Ho Lee Nak-Kyun Soung Joonsung Hwang Cheolju Lee Seung Jun Kim Bo Yeon Kim Hyunjoo Cha-Molstad Source Type: research

Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast
We describe here the optimisation of the α-factor preproleader to improve recombinant enzyme production in S. cerevisiae through two parallel engineering strategies: a bottom-up design over the native α-factor preproleader (αnat) and a top-down design over the fittest evolved signal peptide obtained in our lab (α9H2 leader). The goal was to analyse the effect of mutations accumulated in the signal sequence throughout iterations of directed evolution, or of other reported mutations, and their possible epistatic interactions. Both approaches agreed in the positive synergism of four mutations (Aα...
Source: Cellular and Molecular Life Sciences : CMLS - March 9, 2021 Category: Cytology Authors: Pablo Aza Gonzalo Molpeceres Felipe de Salas Susana Camarero Source Type: research

R-catcher, a potent molecular tool to unveil the arginylome
This study presents the first molecular tool that allows the unbiased identification of arginylated proteins, thereby unlocking the arginylome and provide a new path to disease biomarker discovery.PMID:33687501 | DOI:10.1007/s00018-021-03805-x (Source: Cellular and Molecular Life Sciences : CMLS)
Source: Cellular and Molecular Life Sciences : CMLS - March 9, 2021 Category: Cytology Authors: Taewook Seo Jihyo Kim Ho-Chul Shin Jung Gi Kim Shinyeong Ju Laxman Nawale Goeun Han Hye Seon Lee Geul Bang Jin Young Kim Jeong Kyu Bang Kyung Ho Lee Nak-Kyun Soung Joonsung Hwang Cheolju Lee Seung Jun Kim Bo Yeon Kim Hyunjoo Cha-Molstad Source Type: research

The circadian clock and metabolic homeostasis: entangled networks
Cell Mol Life Sci. 2021 Mar 8. doi: 10.1007/s00018-021-03800-2. Online ahead of print.ABSTRACTThe circadian clock exerts an important role in systemic homeostasis as it acts a keeper of time for the organism. The synchrony between the daily challenges imposed by the environment needs to be aligned with biological processes and with the internal circadian clock. In this review, it is provided an in-depth view of the molecular functioning of the circadian molecular clock, how this system is organized, and how central and peripheral clocks communicate with each other. In this sense, we provide an overview of the neuro-hormona...
Source: Cellular and Molecular Life Sciences : CMLS - March 8, 2021 Category: Cytology Authors: Leonardo Vin ícius Monteiro de Assis Henrik Oster Source Type: research

Thm2 interacts with paralog, Thm1, and sensitizes to Hedgehog signaling in postnatal skeletogenesis
Cell Mol Life Sci. 2021 Mar 8. doi: 10.1007/s00018-021-03806-w. Online ahead of print.ABSTRACTMutations in the intraflagellar transport-A (IFT-A) gene, THM1, have been identified in skeletal ciliopathies. Here, we report a genetic interaction between Thm1, and its paralog, Thm2, in postnatal skeletogenesis. THM2 localizes to primary cilia, but Thm2 deficiency does not affect ciliogenesis and Thm2-null mice survive into adulthood. However, by postnatal day 14, Thm2-/-; Thm1aln/+ mice exhibit small stature and small mandible. Radiography and microcomputed tomography reveal Thm2-/-; Thm1aln/+ tibia are less opaque and have re...
Source: Cellular and Molecular Life Sciences : CMLS - March 8, 2021 Category: Cytology Authors: Bailey A Allard Wei Wang Tana S Pottorf Hammad Mumtaz Brittany M Jack Henry H Wang Luciane M Silva Damon T Jacobs Jinxi Wang Erin E Bumann Pamela V Tran Source Type: research

Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and s...
Source: Cellular and Molecular Life Sciences : CMLS - March 2, 2021 Category: Cytology Authors: Stefanie Heiden Rebecca Siwek Marie-Luise Lotz Sarah Borkowsky Rita Schr öter Pavel Nedvetsky Astrid Rohlmann Markus Missler Michael P Krahn Source Type: research

Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and s...
Source: Cellular and Molecular Life Sciences : CMLS - March 2, 2021 Category: Cytology Authors: Stefanie Heiden Rebecca Siwek Marie-Luise Lotz Sarah Borkowsky Rita Schr öter Pavel Nedvetsky Astrid Rohlmann Markus Missler Michael P Krahn Source Type: research

Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and s...
Source: Cellular and Molecular Life Sciences : CMLS - March 2, 2021 Category: Cytology Authors: Stefanie Heiden Rebecca Siwek Marie-Luise Lotz Sarah Borkowsky Rita Schr öter Pavel Nedvetsky Astrid Rohlmann Markus Missler Michael P Krahn Source Type: research

Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and s...
Source: Cellular and Molecular Life Sciences : CMLS - March 2, 2021 Category: Cytology Authors: Stefanie Heiden Rebecca Siwek Marie-Luise Lotz Sarah Borkowsky Rita Schr öter Pavel Nedvetsky Astrid Rohlmann Markus Missler Michael P Krahn Source Type: research

Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes
In this study, we used expansion microscopy and found the apical polarity determinants of the PAR/aPKC and Crb-complexes to be predominantly targeted to the cell cortex in proximity to the nephrocyte diaphragm, whereas basolateral regulators also accumulate intracellularly. Knockdown of PAR-complex proteins results in severe endocytosis and nephrocyte diaphragm defects, which is due to impaired aPKC recruitment to the plasma membrane. Similar, downregulation of most basolateral polarity regulators disrupts Nephrin localization but had surprisingly divergent effects on endocytosis. Our findings suggest that morphology and s...
Source: Cellular and Molecular Life Sciences : CMLS - March 2, 2021 Category: Cytology Authors: Stefanie Heiden Rebecca Siwek Marie-Luise Lotz Sarah Borkowsky Rita Schr öter Pavel Nedvetsky Astrid Rohlmann Markus Missler Michael P Krahn Source Type: research

Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03791-0. Online ahead of print.ABSTRACTLipids and fatty acids play crucial roles in plant immunity, which have been highlighted over the past few decades. An increasing number of studies have shown that these molecules are pivotal in the interactions between plants and their diverse pathogens. The roles played by plant lipids fit in a wide spectrum ranging from the first physical barrier encountered by the pathogens, the cuticle, to the signalling pathways that trigger different immune responses and expression of defence-related genes, mediated by several lipid molecu...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Ana Rita Cavaco Ana Rita Matos Andreia Figueiredo Source Type: research

Histone acetylation dynamics regulating plant development and stress responses
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03794-x. Online ahead of print.ABSTRACTCrop productivity is directly dependent on the growth and development of plants and their adaptation during different environmental stresses. Histone acetylation is an epigenetic modification that regulates numerous genes essential for various biological processes, including development and stress responses. Here, we have mainly discussed the impact of histone acetylation dynamics on vegetative growth, flower development, fruit ripening, biotic and abiotic stress responses. Besides, we have also emphasized the information gaps wh...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Verandra Kumar Jitendra K Thakur Manoj Prasad Source Type: research

Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03791-0. Online ahead of print.ABSTRACTLipids and fatty acids play crucial roles in plant immunity, which have been highlighted over the past few decades. An increasing number of studies have shown that these molecules are pivotal in the interactions between plants and their diverse pathogens. The roles played by plant lipids fit in a wide spectrum ranging from the first physical barrier encountered by the pathogens, the cuticle, to the signalling pathways that trigger different immune responses and expression of defence-related genes, mediated by several lipid molecu...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Ana Rita Cavaco Ana Rita Matos Andreia Figueiredo Source Type: research

Histone acetylation dynamics regulating plant development and stress responses
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03794-x. Online ahead of print.ABSTRACTCrop productivity is directly dependent on the growth and development of plants and their adaptation during different environmental stresses. Histone acetylation is an epigenetic modification that regulates numerous genes essential for various biological processes, including development and stress responses. Here, we have mainly discussed the impact of histone acetylation dynamics on vegetative growth, flower development, fruit ripening, biotic and abiotic stress responses. Besides, we have also emphasized the information gaps wh...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Verandra Kumar Jitendra K Thakur Manoj Prasad Source Type: research

Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03791-0. Online ahead of print.ABSTRACTLipids and fatty acids play crucial roles in plant immunity, which have been highlighted over the past few decades. An increasing number of studies have shown that these molecules are pivotal in the interactions between plants and their diverse pathogens. The roles played by plant lipids fit in a wide spectrum ranging from the first physical barrier encountered by the pathogens, the cuticle, to the signalling pathways that trigger different immune responses and expression of defence-related genes, mediated by several lipid molecu...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Ana Rita Cavaco Ana Rita Matos Andreia Figueiredo Source Type: research

Histone acetylation dynamics regulating plant development and stress responses
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03794-x. Online ahead of print.ABSTRACTCrop productivity is directly dependent on the growth and development of plants and their adaptation during different environmental stresses. Histone acetylation is an epigenetic modification that regulates numerous genes essential for various biological processes, including development and stress responses. Here, we have mainly discussed the impact of histone acetylation dynamics on vegetative growth, flower development, fruit ripening, biotic and abiotic stress responses. Besides, we have also emphasized the information gaps wh...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Verandra Kumar Jitendra K Thakur Manoj Prasad Source Type: research

Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03791-0. Online ahead of print.ABSTRACTLipids and fatty acids play crucial roles in plant immunity, which have been highlighted over the past few decades. An increasing number of studies have shown that these molecules are pivotal in the interactions between plants and their diverse pathogens. The roles played by plant lipids fit in a wide spectrum ranging from the first physical barrier encountered by the pathogens, the cuticle, to the signalling pathways that trigger different immune responses and expression of defence-related genes, mediated by several lipid molecu...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Ana Rita Cavaco Ana Rita Matos Andreia Figueiredo Source Type: research

Histone acetylation dynamics regulating plant development and stress responses
Cell Mol Life Sci. 2021 Feb 27. doi: 10.1007/s00018-021-03794-x. Online ahead of print.ABSTRACTCrop productivity is directly dependent on the growth and development of plants and their adaptation during different environmental stresses. Histone acetylation is an epigenetic modification that regulates numerous genes essential for various biological processes, including development and stress responses. Here, we have mainly discussed the impact of histone acetylation dynamics on vegetative growth, flower development, fruit ripening, biotic and abiotic stress responses. Besides, we have also emphasized the information gaps wh...
Source: Cellular and Molecular Life Sciences : CMLS - February 27, 2021 Category: Cytology Authors: Verandra Kumar Jitendra K Thakur Manoj Prasad Source Type: research