Cellular and Molecular Life Sciences : CMLS This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Role of chromosomal cohesion and separation in aneuploidy and tumorigenesis
Cell Mol Life Sci. 2024 Feb 22;81(1):100. doi: 10.1007/s00018-024-05122-5.ABSTRACTCell division is a crucial process, and one of its essential steps involves copying the genetic material, which is organized into structures called chromosomes. Before a cell can divide into two, it needs to ensure that each newly copied chromosome is paired tightly with its identical twin. This pairing is maintained by a protein complex known as cohesin, which is conserved in various organisms, from single-celled ones to humans. Cohesin essentially encircles the DNA, creating a ring-like structure to handcuff, to keep the newly synthesized s...
Source: Cellular and Molecular Life Sciences : CMLS - February 22, 2024 Category: Cytology Authors: Debananda Pati Source Type: research

RFX4 is an intrinsic factor for neuronal differentiation through induction of proneural genes POU3F2 and NEUROD1
Cell Mol Life Sci. 2024 Feb 22;81(1):99. doi: 10.1007/s00018-024-05129-y.ABSTRACTProneural genes play a crucial role in neuronal differentiation. However, our understanding of the regulatory mechanisms governing proneural genes during neuronal differentiation remains limited. RFX4, identified as a candidate regulator of proneural genes, has been reported to be associated with the development of neuropsychiatric disorders. To uncover the regulatory relationship, we utilized a combination of multi-omics data, including ATAC-seq, ChIP-seq, Hi-C, and RNA-seq, to identify RFX4 as an upstream regulator of proneural genes. We fur...
Source: Cellular and Molecular Life Sciences : CMLS - February 22, 2024 Category: Cytology Authors: Wonyoung Choi Mu Seog Choe Su Min Kim So Jin Kim Jiyeon Lee Yeongun Lee Sun-Min Lee So Hee Dho Min-Young Lee Lark Kyun Kim Source Type: research

A progesterone derivative linked to a stable phospholipid activates breast cancer cell response without leaving the cell membrane
Cell Mol Life Sci. 2024 Feb 22;81(1):98. doi: 10.1007/s00018-024-05116-3.ABSTRACTIn hormone-responsive breast cancer cells, progesterone (P4) has been shown to act via its nuclear receptor (nPR), a ligand-activated transcription factor. A small fraction of progesterone receptor is palmitoylated and anchored to the cell membrane (mbPR) forming a complex with estrogen receptor alpha (ERα). Upon hormone exposure, either directly or via interaction with ERα, mbPR activates the SRC/RAS/ERK kinase pathway leading to phosphorylation of nPR by ERK. Kinase activation is essential for P4 gene regulation, as the ERK and MSK1 kinase...
Source: Cellular and Molecular Life Sciences : CMLS - February 22, 2024 Category: Cytology Authors: Jofre Font-Mateu Pol Sanlleh í Jes ús Sot Beatriz Abad Nicolas Mateos Juan Andres Torreno-Pina Roberto Ferrari Roni H G Wright Maria F Garcia-Parajo Jes ús Joglar F élix M Goñi Miguel Beato Source Type: research

KIF18A inactivates hepatic stellate cells and alleviates liver fibrosis through the TTC3/Akt/mTOR pathway
In this study, we observed a decrease in the expression of KIF18A in fibrotic liver tissues compared to healthy liver tissues, which exhibited a negative correlation with the activation of HSCs. To elucidate the molecular mechanisms underlying the involvement of KIF18A, we performed in vitro proliferation experiments and established a CCl4-induced liver fibrosis model. Our results revealed that KIF18A knockdown enhanced HSCs proliferation and reduced HSCs apoptosis in vitro. Mouse liver fibrosis grade was evaluated with Masson's trichrome and alpha-smooth muscle actin (α-SMA) staining. In addition, the expression of fibro...
Source: Cellular and Molecular Life Sciences : CMLS - February 19, 2024 Category: Cytology Authors: Hao Zhang Tong Xia Zhijia Xia Huaxin Zhou Zhipeng Li Wei Wang Xiangyu Zhai Bin Jin Source Type: research

Cellular forgetting, desensitisation, stress and ageing in signalling networks. When do cells refuse to learn more?
Cell Mol Life Sci. 2024 Feb 19;81(1):97. doi: 10.1007/s00018-024-05112-7.ABSTRACTRecent findings show that single, non-neuronal cells are also able to learn signalling responses developing cellular memory. In cellular learning nodes of signalling networks strengthen their interactions e.g. by the conformational memory of intrinsically disordered proteins, protein translocation, miRNAs, lncRNAs, chromatin memory and signalling cascades. This can be described by a generalized, unicellular Hebbian learning process, where those signalling connections, which participate in learning, become stronger. Here we review those scenari...
Source: Cellular and Molecular Life Sciences : CMLS - February 19, 2024 Category: Cytology Authors: Tam ás Veres M árk Kerestély Borb ála M Kovács D ávid Keresztes Kl ára Schulc Erik Seitz Zsolt Vassy D ániel V Veres Peter Csermely Source Type: research

Targeted CRISPR activation is functional in engineered human pluripotent stem cells but undergoes silencing after differentiation into cardiomyocytes and endothelium
Cell Mol Life Sci. 2024 Feb 19;81(1):95. doi: 10.1007/s00018-023-05101-2.ABSTRACTHuman induced pluripotent stem cells (hiPSCs) offer opportunities to study human biology where primary cell types are limited. CRISPR technology allows forward genetic screens using engineered Cas9-expressing cells. Here, we sought to generate a CRISPR activation (CRISPRa) hiPSC line to activate endogenous genes during pluripotency and differentiation. We first targeted catalytically inactive Cas9 fused to VP64, p65 and Rta activators (dCas9-VPR) regulated by the constitutive CAG promoter to the AAVS1 safe harbor site. These CRISPRa hiPSC line...
Source: Cellular and Molecular Life Sciences : CMLS - February 19, 2024 Category: Cytology Authors: Elaheh Karbassi Ruby Padgett Alessandro Bertero Hans Reinecke Jordan M Klaiman Xiulan Yang Stephen D Hauschka Charles E Murry Source Type: research