Novel tools to study cell-ECM interactions, cell adhesion dynamics and migration
Curr Opin Cell Biol. 2024 Apr 16;88:102355. doi: 10.1016/j.ceb.2024.102355. Online ahead of print.ABSTRACTIntegrin-mediated cell adhesion is essential for cell migration, mechanotransduction and tissue integrity. In vivo, these processes are regulated by complex physicochemical signals from the extracellular matrix (ECM). These nuanced cues, including molecular composition, rigidity and topology, call for sophisticated systems to faithfully explore cell behaviour. Here, we discuss recent methodological advances in cell-ECM adhesion research and compile a toolbox of techniques that we expect to shape this field in future. W...
Source: Current Opinion in Cell Biology - April 17, 2024 Category: Cytology Authors: Michal Dibus Omkar Joshi Johanna Ivaska Source Type: research
Macropinocytosis at the crossroad between nutrient scavenging and metabolism in cancer
Curr Opin Cell Biol. 2024 Apr 15;88:102359. doi: 10.1016/j.ceb.2024.102359. Online ahead of print.ABSTRACTMacropinocytosis (MP), the actin-dependent bulk uptake of extracellular fluids, plays a central role in nutrient scavenging, allowing cancer cells to sustain their growth in the hypoxic and nutrient-deprived microenvironment often found in solid tumours. The lack of soluble nutrients and several oncogenic signalling pathways, with RAS being the most studied, push MP-dependent internalisation of extracellular proteins, which are then digested in the lysosomes, replenishing the intracellular nutrient pools. This review w...
Source: Current Opinion in Cell Biology - April 16, 2024 Category: Cytology Authors: Elena Rainero Source Type: research
Endoplasmic reticulum - condensate interactions in protein synthesis and secretion
Curr Opin Cell Biol. 2024 Apr 15;88:102357. doi: 10.1016/j.ceb.2024.102357. Online ahead of print.ABSTRACTIn the past decade, a growing amount of evidence has demonstrated that organelles do not act autonomously and independently but rather communicate with each other to coordinate different processes for proper cellular function. With a highly extended network throughout the cell, the endoplasmic reticulum (ER) plays a central role in interorganelle communication through membrane contact sites. Here, we highlight recent evidence indicating that the ER also forms contacts with membrane-less organelles. These interactions c...
Source: Current Opinion in Cell Biology - April 16, 2024 Category: Cytology Authors: Dan T M Nguyen Max Koppers Ginny G Far ías Source Type: research
Macropinocytosis at the crossroad between nutrient scavenging and metabolism in cancer
Curr Opin Cell Biol. 2024 Apr 15;88:102359. doi: 10.1016/j.ceb.2024.102359. Online ahead of print.ABSTRACTMacropinocytosis (MP), the actin-dependent bulk uptake of extracellular fluids, plays a central role in nutrient scavenging, allowing cancer cells to sustain their growth in the hypoxic and nutrient-deprived microenvironment often found in solid tumours. The lack of soluble nutrients and several oncogenic signalling pathways, with RAS being the most studied, push MP-dependent internalisation of extracellular proteins, which are then digested in the lysosomes, replenishing the intracellular nutrient pools. This review w...
Source: Current Opinion in Cell Biology - April 16, 2024 Category: Cytology Authors: Elena Rainero Source Type: research
Endoplasmic reticulum - condensate interactions in protein synthesis and secretion
Curr Opin Cell Biol. 2024 Apr 15;88:102357. doi: 10.1016/j.ceb.2024.102357. Online ahead of print.ABSTRACTIn the past decade, a growing amount of evidence has demonstrated that organelles do not act autonomously and independently but rather communicate with each other to coordinate different processes for proper cellular function. With a highly extended network throughout the cell, the endoplasmic reticulum (ER) plays a central role in interorganelle communication through membrane contact sites. Here, we highlight recent evidence indicating that the ER also forms contacts with membrane-less organelles. These interactions c...
Source: Current Opinion in Cell Biology - April 16, 2024 Category: Cytology Authors: Dan T M Nguyen Max Koppers Ginny G Far ías Source Type: research
Molecular cartography within axons
We describe the similarity and difference in organization of organelles and molecules in varying compartments. Finally, we highlight outstanding questions on the dynamics and localization of various components along the axon that may be answered using orthogonal approaches.PMID:38608424 | DOI:10.1016/j.ceb.2024.102358 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: A King Cada Naoko Mizuno Source Type: research
Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography
Curr Opin Cell Biol. 2024 Apr 11;88:102356. doi: 10.1016/j.ceb.2024.102356. Online ahead of print.ABSTRACTCryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic ...
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: Jonathan Schneider Marion Jasnin Source Type: research
Molecular cartography within axons
We describe the similarity and difference in organization of organelles and molecules in varying compartments. Finally, we highlight outstanding questions on the dynamics and localization of various components along the axon that may be answered using orthogonal approaches.PMID:38608424 | DOI:10.1016/j.ceb.2024.102358 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: A King Cada Naoko Mizuno Source Type: research
Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography
Curr Opin Cell Biol. 2024 Apr 11;88:102356. doi: 10.1016/j.ceb.2024.102356. Online ahead of print.ABSTRACTCryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic ...
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: Jonathan Schneider Marion Jasnin Source Type: research
Molecular cartography within axons
We describe the similarity and difference in organization of organelles and molecules in varying compartments. Finally, we highlight outstanding questions on the dynamics and localization of various components along the axon that may be answered using orthogonal approaches.PMID:38608424 | DOI:10.1016/j.ceb.2024.102358 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: A King Cada Naoko Mizuno Source Type: research
Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography
Curr Opin Cell Biol. 2024 Apr 11;88:102356. doi: 10.1016/j.ceb.2024.102356. Online ahead of print.ABSTRACTCryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic ...
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: Jonathan Schneider Marion Jasnin Source Type: research
Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography
Curr Opin Cell Biol. 2024 Apr 11;88:102356. doi: 10.1016/j.ceb.2024.102356. Online ahead of print.ABSTRACTCryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic ...
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: Jonathan Schneider Marion Jasnin Source Type: research
Molecular cartography within axons
We describe the similarity and difference in organization of organelles and molecules in varying compartments. Finally, we highlight outstanding questions on the dynamics and localization of various components along the axon that may be answered using orthogonal approaches.PMID:38608424 | DOI:10.1016/j.ceb.2024.102358 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 12, 2024 Category: Cytology Authors: A King Cada Naoko Mizuno Source Type: research
The multidimensional roles of intermediate filaments - bridging genetic diversity with form, functions, and targets
Curr Opin Cell Biol. 2024 Apr 10;88:102354. doi: 10.1016/j.ceb.2024.102354. Online ahead of print.NO ABSTRACTPMID:38604107 | DOI:10.1016/j.ceb.2024.102354 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 11, 2024 Category: Cytology Authors: C Patrick Lusk John E Eriksson Source Type: research
The multidimensional roles of intermediate filaments - bridging genetic diversity with form, functions, and targets
Curr Opin Cell Biol. 2024 Apr 10;88:102354. doi: 10.1016/j.ceb.2024.102354. Online ahead of print.NO ABSTRACTPMID:38604107 | DOI:10.1016/j.ceb.2024.102354 (Source: Current Opinion in Cell Biology)
Source: Current Opinion in Cell Biology - April 11, 2024 Category: Cytology Authors: C Patrick Lusk John E Eriksson Source Type: research
