Silencing linc00662 inhibits cell proliferation and colony formation of lung cancer cells via regulating miR-145-5p/PAFAH1B2 axis.
Abstract Lung cancer is the most common cause of cancer-related death in the world. Long non-coding RNAs (lncRNAs) are longer than 200 nucleotides transcripts which are not translated into protein. Linc00662 is overexpressed in lung cancer. However, the roles of linc00662 involved in lung cancer progression are still unknown. In our study, we found that linc00662 was overexpressed in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) by analyzing the TCGA data. We knockdown the linc00662 expression using siRNAs and found that silencing linc00662 significantly inhibited the proliferation and colony ...
Source: Biochemistry and Cell Biology - October 27, 2020 Category: Biochemistry Authors: Xu ZY, Peng J, Shi ZZ, Chen XL, Cheng HZ, Wang H, Wang Y, Wang GP, Jiang W, Peng H Tags: Biochem Cell Biol Source Type: research

Thidiazuron suppresses breast cancer progression via targeting miR-132 and miR-202-5p/PTEN axis mediated dysregulation of PI3K/AKT signaling pathway.
Abstract Chemo-resistance and metastatic disease development are the most common causes of breast cancer recurrence and death. Thidiazuron (TDZ) is a plant growth regulator, its biological role on human and animals has not been yet clarified. In the present study, we investigated the anticancer activity of this plant phytohormone on the drug resistant-triple negative breast cancer MDA-MB-231 cell line. Treatment of the breast cancer cells with TDZ (1-50 μM) caused more stressful environment and induced a significant increase in percentages of active caspases positive cells. In addition, TDZ treatment (5 and 10 ...
Source: Biochemistry and Cell Biology - October 24, 2020 Category: Biochemistry Authors: Ibrahim HI, Ismail MB, Ammar RB, Ahmed E Tags: Biochem Cell Biol Source Type: research

Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKC δ-NLRC4 axis.
Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKCδ-NLRC4 axis. Biochem Cell Biol. 2020 Oct 16;: Authors: Yang F, Yang Y, Zeng W Abstract Anti-double-stranded DNA (anti-dsDNA) has been identified to be closely related to brain inflammatory burden after ischemic stroke. Here, we studied the inflammatory cascade after dsDNA and investigated the mechanisms of its pro-inflammatory role in systemic lupus erythematosus (SLE). The IL-1β and IL-6 levels in serum of SLE patients and controls were evaluate by ELISA, and the caspas...
Source: Biochemistry and Cell Biology - October 16, 2020 Category: Biochemistry Authors: Yang F, Yang Y, Zeng W Tags: Biochem Cell Biol Source Type: research

miR-138-5p knockdown promotes osteogenic differentiation through FOXC1 up-regulation in human bone mesenchymal stem cells.
This study aimed to certify the hypothesis that miR-138-5p is expected to reduced osteodifferentiation of human bone mesenchymal stem cells (hBMSCs) by FOXC1 down-regulation. hBMSCs were separated from bone marrow and osteogenic induction medium was added to stimulate osteogenic differentiation. Flow cytometric analysis was applied to evaluate the expression of cell surface antigens associated with hBMSCs, including CD29, CD44, CD90, CD45 and CD34. qRT-PCR assay and western blot assay were determined to measure the mRNA and protein expression of miR-138-5p, OCN, RUNX2, BSP, ALP and FOXC1. Alkaline phosphatase (ALP) stainin...
Source: Biochemistry and Cell Biology - October 15, 2020 Category: Biochemistry Authors: Zhang L, Liu Y, Feng B, Liu LG, Zhou YC, Tang H Tags: Biochem Cell Biol Source Type: research

Circ_0000043 promotes breast cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the miR-136/ Smad3 axis.
In conclusion, our study demonstrates that circ_0000043 can promote BC progression via regulating the miR-136/Smad3 axis. PMID: 33043682 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - October 11, 2020 Category: Biochemistry Authors: Leng X, Huang G, Ding J, Ma F Tags: Biochem Cell Biol Source Type: research

A Canadian perspective on severe acute respiratory syndrome coronavirus 2 infection and treatment: how prevalent underlying inflammatory disease contributes to pathogenesis.
Abstract The coronavirus disease 2019 (COVID-19), a serious respiratory illness caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has emerged as a global pandemic. Canada reported its first case of COVID-19 on 25th January 2020. By March 2020 the virus had spread within Canadian communities reaching the most frail and vulnerable elderly population in long-term care facilities. The majority of cases were reported in the provinces of Quebec, Ontario, Alberta and British Columbia and the highest mortality was seen among individuals aged 65 years or older. Canada has the highest prevalence an...
Source: Biochemistry and Cell Biology - October 7, 2020 Category: Biochemistry Authors: Willows SD, Alam SB, Sandhu JK, Kulka M Tags: Biochem Cell Biol Source Type: research

LncRNA IDH1-AS1 suppresses cell proliferation and tumor growth in glioma.
Abstract Glioma is a type of brain tumor that is common globally, and is associated with a variety of genetic changes. It has been reported that isocitrate dehydrogenase 1 (IDH1) is overexpressed in glioma and in HeLa cells. The lncRNA IDH1-AS1 is believed to interact with IDH1, and when IDH1-AS1 is overexpressed, HeLa cell proliferation is inhibited. However, the effects of IDH1-AS1 on glioma were relatively unknown. The results from this work show that IDH1-AS1 is downregulated in the glioma tissues. We used primary glioblastoma cell lines U251 and U87-MG to study the effects of IDH1-AS1 on glioma cell growth, i...
Source: Biochemistry and Cell Biology - September 28, 2020 Category: Biochemistry Authors: Wang J, Quan Y, Lv J, Dong Q, Gong S Tags: Biochem Cell Biol Source Type: research

The lncRNA TUG1 promotes cell growth and migration via the TUG1/miR-145-5p/TRPC6 pathway in colorectal cancer.
In conclusion, our study revealed a novel molecular mechanism of TUG1 in CRC progression and suggested the potential of the TUG1/miR-145-5p/TRPC6 pathway to serve as a target for the diagnosis and treatment of CRC. PMID: 32985219 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - September 26, 2020 Category: Biochemistry Authors: Wang X, Bai X, Yan Z, Guo X, Zhang Y Tags: Biochem Cell Biol Source Type: research

Upregulation of Bromodomain PHD ‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival.
Upregulation of Bromodomain PHD‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival. Biochem Cell Biol. 2020 Sep 27;: Authors: Miao J, Zhang M, Huang X, Xu L, Tang R, Wang H, Han S Abstract Bromodomain PHD finger transcription factor (BPTF) is a core subunit of nucleosome-remodeling factor (NURF) complex, which plays an important role in the development of several cancers. However, it is unknown whether BPTF regulates ovarian cancer (OC) progression. Relative expression of BPTF in cell lines and tissues of OC were measured by Western blot an...
Source: Biochemistry and Cell Biology - September 26, 2020 Category: Biochemistry Authors: Miao J, Zhang M, Huang X, Xu L, Tang R, Wang H, Han S Tags: Biochem Cell Biol Source Type: research

AC006262.5/miR-7855-5p/BPY2C axis facilitates hepatocellular carcinoma proliferation and migration.
Abstract Hepatocellular carcinoma (HCC) is a considerable threat to human life, and patients with HCC are usually diagnosed in the later stages. Although treatment for HCC has recently advanced rapidly, novel targets for HCC are still desperately needed, especially for precision medicine. Here, we identified an HCC enriched long non-coding RNA, AC006262.5, that promoted the proliferation, migration, and invasion of HCC both in vitro and in vivo. In addition, our results revealed that AC006262.5 bound to and regulated miR-7855-5p, a tumor suppressive miRNA in HCC. Moreover, our data illustrated that AC006262.5 regu...
Source: Biochemistry and Cell Biology - September 20, 2020 Category: Biochemistry Authors: Chen F, Wang Y, Cheng Y, Shi H, Li H, Jia M, Sha S, Dong L Tags: Biochem Cell Biol Source Type: research

Effect of bovine lactoferrin on prevention of late-onset sepsis in infants < 1500g: a pooled analysis of individual patient data from two randomized controlled trials.
Effect of bovine lactoferrin on prevention of late-onset sepsis in infants
Source: Biochemistry and Cell Biology - September 14, 2020 Category: Biochemistry Authors: Ochoa TJ, Loli S, Mendoza K, Carcamo C, Bellomo S, Cam L, Castañeda A, Campos M, Jacobs J, Cossey V, Zegarra J Tags: Biochem Cell Biol Source Type: research

Automated classification of mitotic catastrophe by use of the centromere fragmentation morphology.
We present an automated 2-step system for segmentation of cells exhibiting centromere fragmentation. The first step segments individual cells from clumps. We added two new terms, Weighted Local Repelling term (WLRt) and Weighted Gradient term (WGt), in the energy functional of the traditional Chan & Vese based level set method. WLRt was used to generate a repelling force when contours of adjacent cells merged and then penalized the overlap. WGt enhances gradients between overlapping cells. The second step consists of a new algorithm, SBaN (Shape-Based analysis of each nuclei), which extracts features like circularity, ...
Source: Biochemistry and Cell Biology - September 8, 2020 Category: Biochemistry Authors: Roy K, Lewis CW, Chan G, Bhattacharjee D Tags: Biochem Cell Biol Source Type: research

Effects of oral bovine lactoferrin on a mouse model of inflammation associated colon cancer.
Abstract Both ulcerative colitis and colonic Crohn's disease patients have a significantly increased risk of developing colorectal cancer. bLF is reported to inhibit the development of colon cancer in rats and mice, and in a placebo controlled trial, ingestion of bLF inhibited the growth of intestinal polyps. In addition, in a case study a Crohn's disease patient was reported to have remained in remission for over 7 years while ingesting 1 gram of bLF daily. Thus, bLF has an inhibitory effect on colon carcinogenesis, and it may also promote remission of Crohn's disease. The purpose of the present study was to begi...
Source: Biochemistry and Cell Biology - September 8, 2020 Category: Biochemistry Authors: Tanaka H, Gunasekaran S, Saleh DM, Alexander WT, Alexander DB, Ohara H, Tsuda H Tags: Biochem Cell Biol Source Type: research

Lactoferrin and hematoma detoxification after intracerebral hemorrhage.
Abstract This review discusses the role of lactoferrin (LTF) in detoxifying hematoma after intracerebral hemorrhage (ICH). Subsequent to ICH, neutrophils enter the ICH-affected brain, where they release various granule content, including LTF. LTF is an iron-binding glycoprotein that binds Fe3+ with high affinity. Unlike other iron binding proteins, LTF can retain Fe3+ at the low pH associated with inflamed tissue. LTF's ability to sequester Fe3+ is of particular importance to ICH pathogenesis, as large quantities of free iron, which is pro-oxidative and pro-inflammatory are generated in the ICH-affected brain due ...
Source: Biochemistry and Cell Biology - September 3, 2020 Category: Biochemistry Authors: Zhao X, Kruzel ML, Aronowski J Tags: Biochem Cell Biol Source Type: research

Antibiofilm activity of lactoferrin-derived synthetic peptides against Pseudomonas aeruginosa PAO1.
Abstract Many pathogenic bacteria can protect themselves from the effects of antibiotics and the host immune response system by forming biofilms. Eradication of bacterial biofilms typically requires much higher concentrations of antibiotics, than are normally needed to kill cultured planktonic cells, raising serious clinical concerns. In an attempt to try to prevent the formation of biofilms or to break up existing biofilms of pathogenic bacteria, herein we have used the standard crystal violet assay as well as the Calgary biofilm device, to test several lactoferrin- and lactoferricin-derived antimicrobial peptide...
Source: Biochemistry and Cell Biology - August 31, 2020 Category: Biochemistry Authors: Ramamourthy G, Vogel HJ Tags: Biochem Cell Biol Source Type: research

Transcriptional regulation of human abraxas brother protein 1 expression by yin yang 1.
In this study, we identified and characterized the core regulatory elements of the human ABRO1 gene and mapped them to the ABRO1 promoter region. Additionally, 5' rapid amplification of cDNA ends revealed that the transcriptional start site (TSS) was located -13 bp upstream from the start codon. Reporter gene, chromatin immunoprecipitation, and electrophoretic mobility shift assays demonstrated that ABRO1 transcription was regulated through cis-acting elements located in the region -89 to -59 bp upstream of the ABRO1 TSS and that these elements were targeted by yin yang 1 transcription factor (YY1). Moreover, YY1 overexpre...
Source: Biochemistry and Cell Biology - August 25, 2020 Category: Biochemistry Authors: Song P, Hong J, Wang Y, Yao X, Zhan Y, Yin R, Yu M, Li C, Yang X, Ge C Tags: Biochem Cell Biol Source Type: research

Dose Escalation Study of Bovine Lactoferrin in Preterm Infants: Getting the Dose Right.
Abstract Lactoferrin as a nutritional enteral supplement has emerged as a novel preventative therapy against serious infections in preterm infants, though neonatal studies have demonstrated variable results in part due to the lack of pharmacokinetic data and differences in products. We conducted a prospective, dose escalation (100, 200, and 300 mg/kg/day) safety study of bovine lactoferrin (Glanbia Nutritionals, USA) dissolved in sterile water (100 mg/ml) for 30 days in preterm infants
Source: Biochemistry and Cell Biology - August 25, 2020 Category: Biochemistry Authors: Kaufman DA, Berenz A, Itell HL, Conaway M, Blackman A, Nataro JP, Permar SR Tags: Biochem Cell Biol Source Type: research

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin.
Abstract Lactoferrin supplementation may help prevent infections in preterm infants but efficacy has varied with different dosing and products. We assessed the absorption and excretion of bovine lactoferrin (bLF) in thirty-one infants receiving 100, 200, or 300 mg/kg/day of enteral bLF for 30 days. BLF and human lactoferrin (hLF) in infant saliva, blood, urine, and stool and expressed or donor breast milk (EBM, DBM) collected before treatment initiation, study day 22, and one week after treatment cessation were measured by ELISA. During treatment, BLF was absorbed from the gastrointestinal tract, detected in plasm...
Source: Biochemistry and Cell Biology - August 24, 2020 Category: Biochemistry Authors: Itell HL, Berenz A, Mangan RJ, Permar SR, Kaufman DA Tags: Biochem Cell Biol Source Type: research

hCINAP is a potential direct HIF-1 target gene and is required for hypoxia-induced EMT and apoptosis in cervical cancer cells.
Abstract The early metastasis of cervical cancer is a multi-step process requiring the cancer cells to adapt to the signal input from different tissue environments, including hypoxia. Hypoxia-induced epithelial-to-mesenchymal transition (EMT) plays a critical role in the acquisition of the ability to invade surrounding tissue. However, the molecular mechanism underlying EMT in cervical cancer remains to be elucidated. Herein, we showed that HIF‑1α and ARNT are recruited to the hCINAP promoter and initiate hCINAP expression in hypoxia. Ablation of hCINAP decreased the migratory capacity and EMT of cervical ...
Source: Biochemistry and Cell Biology - August 22, 2020 Category: Biochemistry Authors: Zhang Y, Jiang L, Qin N, Cao M, Liang X, Wang R Tags: Biochem Cell Biol Source Type: research

ELFIN, the United Kingdom preterm lactoferrin trial: interpretation and future questions.
Abstract Previous studies suggested that supplemental bovine lactoferrin (BLF) given to preterm infants (2200 preterm infants. ELFIN did not demonstrate a reduction in LOS or NEC, or several other clinically important measures. 316 (29%) of 1093 infants in the intervention group developed late-onset sepsis versus 334 (31%) of 1089 in the control group with an adjusted risk ratio of 0·95 (95% CI 0·86-1·04; p=0· 233). Reasons for the differences in ELFIN trial results and other studies may include population differences, the routine use of antifungals in the UK, timing of administration o...
Source: Biochemistry and Cell Biology - August 22, 2020 Category: Biochemistry Authors: Berrington JE, McGuire W, Embleton ND Tags: Biochem Cell Biol Source Type: research

TET2 expedites coronary heart disease by promoting microRNA-126 expression and inhibiting the E2F3/PI3K/AKT axis.
Abstract DNA demethylases of the ten-eleven translocation (TET) family serve as tumor suppressors in various human cancers but their pathogenic contributions in coronary heart disease (CHD) progression remain unclear. Here we report that TET2 is transcriptionally upregulated in CHD patients where it demonstrates diagnostic efficiency. Mechanistic investigations revealed that TET2 facilitated inflammatory responses and cardiomyocyte apoptosis in rats through demethylation of microRNA-126 (miR-126) promoter. This interaction led to sequestration of miR-126 from its target E2F transcription factor 3 (E2F3), contribut...
Source: Biochemistry and Cell Biology - August 19, 2020 Category: Biochemistry Authors: Xu Y Tags: Biochem Cell Biol Source Type: research

Astragaloside IV enhanced carboplatin sensitivity in prostate cancer by suppressing AKT/NF- κB signaling pathway.
Astragaloside IV enhanced carboplatin sensitivity in prostate cancer by suppressing AKT/NF-κB signaling pathway. Biochem Cell Biol. 2020 Aug 19;: Authors: He Y, Zhang Q, Chen H, Guo Q, Zhang L, Zhang Z, Li Y Abstract In our study, we explored the effect of AgIV on carboplatin chemotherapy in prostate cancer cell lines in vitro and in vivo. Cell viability assay, colony formation assay, flow cytometry, western blot, immunohistochemistry (IHC), immunofluorescence and tumor xenograft growth assay were conducted. We found that AgIV significantly decreased the half maximal inhibitory concentration (IC...
Source: Biochemistry and Cell Biology - August 18, 2020 Category: Biochemistry Authors: He Y, Zhang Q, Chen H, Guo Q, Zhang L, Zhang Z, Li Y Tags: Biochem Cell Biol Source Type: research

Evidence from systematic reviews of randomized trials on enteral lactoferrin supplementation in preterm neonates.
Abstract In this commentary, we summarize the current evidence from randomized controlled trials on enteral lactoferrin supplementation in preterm neonates. Our recently completed systematic review includes 12 randomized controlled trials performed all over the world. Our meta-analysis suggests clinical benefit in decreasing late-onset sepsis, late-onset fungal sepsis, length of stay in the hospital and urinary tract infections. There were no adverse effects. There was no statistically significant decrease in necrotizing enterocolitis, mortality or neurodevelopmental impairment in lactoferrin supplemented preterm ...
Source: Biochemistry and Cell Biology - July 27, 2020 Category: Biochemistry Authors: Pammi M, Preidis GA, Tarnow-Mordi WO Tags: Biochem Cell Biol Source Type: research

Biological activities of commercial bovine lactoferrin sources.
g R Abstract Lactoferrin (Lf) samples from several manufacturers were evaluated in vitro. The purity and protein form of each Lf were examined by SDS-PAGE, Western blot, and proteomics analysis. Assays were conducted to evaluate uptake of Lfs and iron from Lfs by enterocytes as well as Lf bioactivities, including effects on intestinal cell proliferation and differentiation, IL-18 secretion, TGF-β1 transcription, and growth of enteropathogenic Escherichia coli (EPEC). Composition of the Lfs varied; some only contain a major Lf band (~80 kDa), and some also contain minor forms. All Lfs and iron from the Lfs wer...
Source: Biochemistry and Cell Biology - July 23, 2020 Category: Biochemistry Authors: Lönnerdal B, Du X, Jiang R Tags: Biochem Cell Biol Source Type: research

Beyond the Genome: Challenges and Potential for Epigenetics-driven Therapeutic Approaches in Pulmonary Arterial Hypertension.
Abstract Pulmonary arterial hypertension (PAH) is a devastating disease of the cardiopulmonary system caused by the narrowing of the pulmonary arteries leading to increased vascular resistance and blood pressure. This leads to right ventricle remodeling, dysfunction and eventually death. While conventional therapies have largely focused on targeting vasodilation, other pathological features of PAH including aberrant inflammation, mitochondrial dynamics, cell proliferation, and migration have not been well explored. Thus despite some recent improvements in PAH treatment, the life expectancy and quality of life for ...
Source: Biochemistry and Cell Biology - July 23, 2020 Category: Biochemistry Authors: Hsu JY, Major JL, Riching AS, Sen R, Pires da Silva J, Bagchi RA Tags: Biochem Cell Biol Source Type: research

Long non-coding RNA AWPPH enhances malignant phenotypes in nasopharyngeal carcinoma via silencing PTEN through interacting with LSD1 and EZH2.
In this study, AWPPH expression was markedly upregulated in NPC cells. Besides, the loss- and gain-of-function assays indicated that AWPPH facilitated cell proliferation and migration while restrained cell apoptosis in NPC cells. Moreover, cytoplasmic AWPPH was predicted to share a common RNA-binding protein, IGF2BP1, with LSD1. And the interaction of IGF2BP1 with both AWPPH and LSD1 mRNA was certified in NPC cells, while AWPPH stabilized LSD1 mRNA to enhance the expression of LSD1 in NPC through such interaction. Furthermore, nuclear AWPPH repressed PTEN expression through recruiting EZH2 and LSD1 to PTEN promoter in NPC ...
Source: Biochemistry and Cell Biology - July 13, 2020 Category: Biochemistry Authors: Guo D, Liu F, Zhang L, Bian N, Liu L, Kong L, Hu Q, Wang Z Tags: Biochem Cell Biol Source Type: research

MiR-448 targets BLC2 and inhibits the growth of pituitary adenoma cells.
Abstract There is an increasing body of evidence indicating the important roles of miRNAs in the progression of pituitary adenoma. Recent studies have shown decreased expression and tumor suppressive function of miR-448 in cancers; however, the clinical significance of miR-448 in pituitary adenoma has remained largely unknown. In our study, we found that miR-448 was down-regulated in pituitary adenoma tissues and cell lines. Overexpression of miR-448 significantly inhibited the proliferation and migration of pituitary adenoma cells. Increased cell apoptosis was also observed with overexpression of miR-448. To furt...
Source: Biochemistry and Cell Biology - July 9, 2020 Category: Biochemistry Authors: He C, Yang J, Ding J, Li S, Wu H, Zhou F, Teng L, Yang J Tags: Biochem Cell Biol Source Type: research

A prospective on multiple biological activities of lactoferrin contributing into piglet welfare.
Abstract Piglets, especially for weaning piglets, due to rapid growth, physiological immaturity and gradual reduction of maternal antibodies, showed a lower immunity level and higher morbidity, which affects seriously growth performance and economic benefits. It is important that the piglets are how to adapt to nutrient digestion and absorption, and to develop the intestinal function and the colonization of gut microbiota during early stage as soon as possible. Lactoferrin is a natural glycoprotein polypeptide that is differentiated from the transferrin family. It is widely found in mucosal secretions such as sali...
Source: Biochemistry and Cell Biology - June 26, 2020 Category: Biochemistry Authors: Hao Y, Wang J, Teng D, Wang X, Mao R, Yang N, Ma X Tags: Biochem Cell Biol Source Type: research

Minireview: Lactoferrin, a versatile natural antimicrobial glycoprotein which modulates host innate immunity.
Abstract Lactoferrin is a multifunctional protein found in the secretions of mammals. The antimicrobial activity of lactoferrin was the first to be discovered and was assumed to be solely dependent on its iron-chelating ability. However, lactoferrin has been reported to display proteolytic activity towards bacterial virulence factors and to modulate the host defence by stimulating the immune system and balancing pathogen-induced inflammation. Here, we review the current understandings of the antimicrobial effect, interaction with host cells and innate immune modulation of lactoferrin and put forward this moonlight...
Source: Biochemistry and Cell Biology - June 24, 2020 Category: Biochemistry Authors: Dierick M, Vanrompay D, Devriendt B, Cox E Tags: Biochem Cell Biol Source Type: research

MiR-27b-3p suppresses glioma development via targeting YAP1.
Abstract Previous studies have reported that miRNAs are involved in the progression of glioma, and that miR-27b-3p is involved in a variety of cancers. However, whether miR-27b-3p has a role in glioma is still unknown. Here, we demonstrated that miR-27b-3p is downregulated in glioma, and this is associated with the development of glioma. Overexpression of miR-27b-3p in glioma cells inhibits cell proliferation and migration, and induces cell apoptosis, which suppresses the progression of glioma. Furthermore, in our study, overexpression of miR-27b-3p also inhibited the growth of xenografted glioma tumors in-vivo. F...
Source: Biochemistry and Cell Biology - June 21, 2020 Category: Biochemistry Authors: Miao W, Li N, Gu B, Yi G, Su Z, Cheng H Tags: Biochem Cell Biol Source Type: research

Binding of lactoferrin to the surface of low-density lipoproteins modified by myeloperoxidase prevents intracellular cholesterol accumulation by human blood monocytes.
Abstract Myeloperoxidase (MPO) is unique heme-containing peroxidase which can catalyze formation of hypochlorous acid (HOCl). Strong interaction of MPO with low-density lipoproteins (LDL) promotes proatherogenic modification of LDL by HOCl. The so-called MPO-modified LDL (Mox-LDL) accumulates in macrophages with formation of foam cells, which is the pathognomic symptom of atherosclerosis. A promising approach to prophylaxis and therapy of atherosclerosis is searching for remedies preventing modification or accumulation of LDL in macrophages. Lactoferrin (LF) has several application points in obesity pathogenesis. ...
Source: Biochemistry and Cell Biology - June 15, 2020 Category: Biochemistry Authors: Vasilyev VB, Sokolov AV, Kostevich VA, Elizarova AY, Gorbunov NP, Panasenko OM Tags: Biochem Cell Biol Source Type: research

Comparative effect of bovine buttermilk, whey, and lactoferrin on the innate immunity receptors and oxidative status of intestinal epithelial cells.
Sánchez L Abstract Milk contains active molecules with important functional properties as the defensive proteins; among them are the whey protein lactoferrin and proteins of the milk fat globule membrane (MFGM) present in buttermilk. The aim of this study has been to investigate the effect of lactoferrin, whey and buttermilk as modulators of intestinal innate immunity and oxidative stress on intestinal epithelial cells, to evaluate its potential use for the development of functional foods. Innate immune Toll-like receptors (TLR2, TLR4, and TLR9) mRNA expression, lipid peroxidation (MDA+4-HDA) and protein c...
Source: Biochemistry and Cell Biology - June 12, 2020 Category: Biochemistry Authors: Buey B, Bellés A, Latorre E, Abad I, Pérez MD, Grasa L, Mesonero JE, Sánchez L Tags: Biochem Cell Biol Source Type: research

Inhibition of long noncoding RNA MALAT1 suppresses high glucose-induced apoptosis and inflammation in human umbilical vein endothelial cells by suppressing the NF- κB signaling pathway.
In conclusion, our study demonstrated that MALAT1 was upregulated in HG-induced HUVECs, and inhibition of MALAT1 could inhibit HG-induced apoptosis and inflammation in HUVECs by suppression of the NF-κB signaling pathway. PMID: 32502356 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - June 4, 2020 Category: Biochemistry Authors: Gong YP, Zhang YW, Su XQ, Gao HB Tags: Biochem Cell Biol Source Type: research

Lactoferrin levels in cervical fluid from in vitro fertilization (IVF) patients. Correlation with IVF parameters.
Abstract Since our previous results suggest that LF might have roles in the reproductive process and its levels might change in the female tract as a response to various factors, the aim of this investigation was to assess whether LF levels in cervical secretions correlate with reproductive parameters from in vitro fertilization (IVF) patients. Cervical fluid samples were obtained from 34 women under 40 years old enrolled for assisted reproduction techniques and LF concentration was measured. The mean total protein concentration in all cervical fluid samples was 842.8±116.9 µg/ml. The mean concentrati...
Source: Biochemistry and Cell Biology - May 30, 2020 Category: Biochemistry Authors: Massa E, Pelusa F, Lo Celso A, Madariaga MJ, Filocco L, Morente C, Ghersevich SA Tags: Biochem Cell Biol Source Type: research

New Insights into the Systemic Effects of Oral Lactoferrin: Transcriptome Profiling.
Abstract The immunomodulatory nature of lactoferrin (LF) derives from its ability to bridge innate and adaptive immunity in obtaining physiological equilibrium. LF is an attractive molecule for treatment of diseases that compromise immune homeostasis. Oral delivery is a preferable method for LF administration; however, its bioavailability is affected by protein degradation and absorption. The aim of this study was to evaluate the systemic effects of oral and intravenous (IV) administered recombinant human LF (rhLF) on blood cell transcriptome profiling. Rats were administered with a single dose of rhLF by gavage o...
Source: Biochemistry and Cell Biology - May 29, 2020 Category: Biochemistry Authors: Kruzel ML, Olszewska P, Pazdrak B, Krupinska AM, Actor JK Tags: Biochem Cell Biol Source Type: research

Mistranslating tRNA identifies a deleterious S213P mutation in the Saccharomyces cerevisiae eco1-1 allele.
Abstract Mistranslation occurs when an amino acid not specified by the standard genetic code is incorporated during translation. Since the ribosome does not read the amino acid, tRNA variants aminoacylated with a non-cognate amino acid or containing a non-cognate anticodon dramatically increase the frequency of mistranslation. In a systematic genetic analysis, we identified a suppression interaction between tRNASerUGG, G26A, which mistranslates proline codons by inserting serine, and eco1-1, a temperature sensitive allele of the gene encoding an acetyltransferase required for sister chromatid cohesion. The suppres...
Source: Biochemistry and Cell Biology - May 29, 2020 Category: Biochemistry Authors: Zhu Y, Berg MD, Yang P, Loll-Krippleber R, Brown GW, Brandl CJ Tags: Biochem Cell Biol Source Type: research

The N-glycans of lactoferrin: More than just a sweet decoration.
Abstract Nearly all extracellular proteins undergo posttranslational modification with sugar chains during their transit through the endoplasmic reticulum and the Golgi apparatus. These "sweet" modifications not only influence the activity of its carrier protein, but they themselves often have bioactivity, independent of the carrier function. Lactoferrin belongs to the group of glycoproteins and is modified with several different N-glycans. This review summarizes several studies dealing with the diverse glycosylation patterns of lactoferrin from different origins and the potential impact of these posttra...
Source: Biochemistry and Cell Biology - May 27, 2020 Category: Biochemistry Authors: Zlatina K, Galuska SP Tags: Biochem Cell Biol Source Type: research

FOXD3 inhibits cell proliferation, migration and invasion in nasopharyngeal carcinoma through regulation of the PI3K/Akt pathway.
In conclusion, FOXD3 negatively mediated the PI3K/Akt pathway to restrain the processes involved in C6661 cell pathology. These findings further exposed the function and downstream axis of FOXD3 in NPC and displayed a promising new target for NPC therapy. PMID: 32459973 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - May 26, 2020 Category: Biochemistry Authors: Xie X, Xiong G, Chen W, Fu H, Li M, Cui X Tags: Biochem Cell Biol Source Type: research

YAP confers resistance to vandetanib in medullary thyroid cancer.
Abstract Medullary thyroid cancer (MTC) is the third most common thyroid cancer. RET (Rearranged in Transformation) gene mutations are considered as one of the major drivers of MTC. Vandetanib suppresses RET activity, and has shown promise in clinical trials. Unfortunately, acquired resistance to vandetanib has been observed in MTC, although the mechanism was largely unknown. We investigated the critical role of YAP (Yes-Associated Protein) on vandetanib resistance in MTC. For this, TT cells (medullary thyroid cancer cells) were treated with vandetanib for 3 months to generate a vandetanib-resistant cell line (TT-...
Source: Biochemistry and Cell Biology - May 24, 2020 Category: Biochemistry Authors: Wang H, Tang J, Su Z Tags: Biochem Cell Biol Source Type: research

Circ_BMP2K enhances the regulation of miR-455-3p on its target gene SUMO1 to inhibit the activation of cardiac fibroblasts.
Abstract It has been found that some circular RNAs (circRNA) are abnormally expressed in the process of myocardial fibrosis, but the mechanism of them is still unknown. In the process of inducing cardiac fibroblast (CF) activation with TGF-β1 or Ang II, circRNA circ_BMP2K and miR-455-3p expression was significantly inhibited, and SUMO1 expression was promoted. Dual luciferase reporter gene assay, RIP and pull down assay showed that miR-455-3p bound with circ_BMP2K directly, and they promoted each other's expression. SUMO1 was a target gene of miR-455-3p, and circ_BMP2K enhanced the inhibition of miR-455-3p on...
Source: Biochemistry and Cell Biology - May 14, 2020 Category: Biochemistry Authors: Zhang L, Bian YF, Bai R, Song XS, Liang B, Xiao CS Tags: Biochem Cell Biol Source Type: research

Lactoferrin Reduces Mycobacterial Trehalose 6,6'-dimycolate Induced M1-type Inflammation and Permits Fluoroquinolone Entry to Granulomas.
Abstract Primary Mycobacterium tuberculosis (Mtb) infection results in the formation of a densely packed granulomatous response that essentially limits entry and efficacy of immune effector cells. Furthermore, the physical nature of the granuloma does not readily permit entry of therapeutic agents to sites where organisms reside. The Mtb cell wall mycolic acid, trehalose 6,6'-dimycolate (TDM), is a physiologically-relevant molecule to model macrophage mediated events during establishment of the tuberculosis-induced granuloma pathogenesis. No current therapeutic modalities focus to modulate host immune responses to...
Source: Biochemistry and Cell Biology - May 12, 2020 Category: Biochemistry Authors: Nguyen TKT, Niaz Z, d'Aigle J, Hwang SA, Kruzel ML, Actor JK Tags: Biochem Cell Biol Source Type: research

Tetramethylpyrazine ameliorates hepatic fibrosis through autophagy-mediated inflammation.
CONCLUSIONS: TMP exerts therapeutic effects in hepatic fibrosis mainly through promoting autophagy to ameliorate inflammation by inhibiting the AKT-mTOR signalling pathway, providing a new perspective for the treatment of chronic liver diseases. PMID: 32383631 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - May 7, 2020 Category: Biochemistry Authors: Hu Z, Su H, Zeng Y, Lin C, Guo Z, Zhong F, Jiang K, Yuan G, He S Tags: Biochem Cell Biol Source Type: research

Chondroitin polymerizing factor (CHPF) contributes to malignant proliferation and migration of hepatocellular carcinoma cells.
In conclusion, we demonstrated that CHPF plays important roles in the development and progression of HCC, and high expression levels of HCC may be related with poorer prognosis. The results from this study may provide a potential therapeutic target for HCC treatment. PMID: 32383983 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - May 7, 2020 Category: Biochemistry Authors: Xu Q, Lin W, Tao C, Huang X, Li J Tags: Biochem Cell Biol Source Type: research

Long noncoding RNA KCNQ1OT1 is correlated with human breast cancer cell development through inverse regulation of hsa-miR-107.
Abstract In this work, we investigated the expression pattern and regulatory function of long noncoding RNA (lncRNA) KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in breast cancer. We found that KCNQ1OT1 was significantly upregulated in breast cancer cell lines. In lentiviral-transduced BT-549 and HCC1599 cells, KCNQ1OT1 knockdown impaired cancer cell functions, including in vitro proliferation and migration, and in vivo transplant growth. The possible sponging target of KCNQ1OT1, human microRNA-107 (hsa-miR-107), was confirmed to be bound by KCNQ1OT1, and was upregulated in breast cancer cells with KCNQ...
Source: Biochemistry and Cell Biology - May 6, 2020 Category: Biochemistry Authors: Wu Y, Bi QJ, Han R, Zhang Y Tags: Biochem Cell Biol Source Type: research

Long non-coding RNA DSCAM-AS1 upregulates USP47 expression through sponging miR-101-3p to accelerate osteosarcoma progression.
In this study, our findings proved that DSCAM-AS1 was highly expressed in OS cells. DSCAM-AS1 knockdown suppressed cell proliferation, migration and invasion, as well as induced cell apoptosis in OS. Additionally, DSCAM-AS1 suppression inactivated the Wnt/β-catenin pathway. Moreover, researches of molecular mechanism confirmed that DSCAM-AS1 could function as a sponge for miR-101-3p and Ubiquitin-specific peptidase 47 (USP47) was the target gene of miR-101-3p. Furthermore, a negative relationship between miR-101-3p and DSCAM-AS1 or USP47 was discovered. Rescue assays suggested that DSCAM-AS1 regulated OS progression t...
Source: Biochemistry and Cell Biology - May 6, 2020 Category: Biochemistry Authors: Zhang S, Ding L, Gao F, Fan H Tags: Biochem Cell Biol Source Type: research

Antiviral Effects of Bovine Lactoferrin on Human Norovirus.
Abstract Human noroviruses cause significant morbidity and mortality worldwide but lack approved antivirals or vaccines to treat or prevent infections. The recent development of two cell culture systems in human transformed B cells (BJABs) and non-transformed human intestinal enteroids cultures overcomes a main limitation in identifying molecules with anti-norovirus activities. Lactoferrin is an iron-binding glycoprotein found in the milk of most mammals with broad spectrum antimicrobial activities, including against the related murine norovirus in cell culture. Its ingestion also reduced the incidence of infectio...
Source: Biochemistry and Cell Biology - April 28, 2020 Category: Biochemistry Authors: Oda H, Kolawole AO, Mirabelli C, Wakabayashi H, Tanaka M, Yamauchi K, Abe F, Wobus CE Tags: Biochem Cell Biol Source Type: research

LINP1 Promotes the Progression of Cervical Cancer by Scaffolding EZH2, LSD1 and DNMT1 to Inhibit the Expression of KLF2 and PRSS8.
Abstract A growing body of evidence demonstrates that long non-coding RNAs (lncRNAs) are correlated with various cancers. LncRNA LINP1 has been proven as a key factor of malignant tumor development. However, it is still unclear about the role of LINP1 in cervical cancer (CC). In our research, a prominent increase of LINP1 was found in CC tissues in comparison with adjacent normal tissues. What's more, LINP1 expression was identified to be upregulated in CC cells compared with human cervical epithelial cell lines (HUCEC). Surprisingly, the downregulation of LINP1 markedly alleviated the proliferation and promoted t...
Source: Biochemistry and Cell Biology - April 28, 2020 Category: Biochemistry Authors: Wu L, Gong Y, Yan T, Zhang H Tags: Biochem Cell Biol Source Type: research

Customizable Live-Cell Imaging Chambers for Multimodal and Multiplex Fluorescence Microscopy.
Abstract Using multiple imaging modalities while performing independent experiments in parallel can greatly enhance the throughput of microscopy-based research, but requires provision of appropriate experimental conditions in a format that meets the microscopy's optical requirements. Although customized imaging chambers can meet these challenges, the difficulty of manufacturing custom chambers and the relatively high cost and design inflexibility of commercial chambers has limited the adoption of this approach. Herein, we demonstrate the use of 3D printing to produce inexpensive, customized live-cell imaging chamb...
Source: Biochemistry and Cell Biology - April 26, 2020 Category: Biochemistry Authors: Tepperman AL, Zheng DJ, Abou Taka M, Vrieze A, Lam AL, Heit B Tags: Biochem Cell Biol Source Type: research

MicroRNA-518-3p suppresses cell proliferation, invasion and migration in colorectal cancer via targeting TRIP4.
Abstract MicroRNA (miR)-518-3p has been shown to function as a tumor suppressor. The present study was undertaken to investigate the effects of miR-518-3p in colorectal cancer (CRC). The miR-518-3p mimic, mimic negative control (NC), miR-518-3p inhibitor, inhibitor-NC, ShRNA-TRIP4 and ShRNA-NC vectors were transfected into SW480 cells using Lipofectamine 2000. Cell viability was detected using CCK 8. The clone-formation, invasive and migratory capacities were separately assessed by plate clone formation, Transwell and wound healing assays. Relative mRNA and protein levels were detected using RT qPCR and western bl...
Source: Biochemistry and Cell Biology - April 15, 2020 Category: Biochemistry Authors: Yang H, Ren J, Bai Y, Jiang J, Xiao S Tags: Biochem Cell Biol Source Type: research

In Memoriam / In Memoriam.
PMID: 32266831 [PubMed - in process] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - March 31, 2020 Category: Biochemistry Authors: Germida JJ, Kettley S Tags: Biochem Cell Biol Source Type: research