Duloxetine suppresses BMP-4-induced release of osteoprotegerin via inhibition of the SMAD signaling pathway in osteoblasts
Biochem Cell Biol. 2021 Mar 1. doi: 10.1139/bcb-2020-0347. Online ahead of print.ABSTRACTDuloxetine, a selective serotonin-norepinephrine reuptake inhibitor, is currently recommended to use in the treatment of chronic painful disorders such as fibromyalgia, chronic musculoskeletal pain and diabetic peripheral neuropathy. We previously demonstrated that bone morphogenetic protein-4 (BMP-4) stimulates osteoprotegerin (OPG) production in osteoblast-like MC3T3-E1 cells, and that p70 S6 kinase positively regulates the OPG synthesis. The present study is aimed to investigate the effect of duloxetine on BMP-4-stimulated OPG synth...
Source: Biochemistry and Cell Biology - March 1, 2021 Category: Biochemistry Authors: Junko Tachi Takashi Onuma Shinobu Yamaguchi Woo Kim Tomoyuki Hioki Rie Matsushima-Nishiwaki Kumiko Tanabe Haruhiko Tokuda Osamu Kozawa Hiroki Iida Source Type: research

Duloxetine suppresses BMP-4-induced release of osteoprotegerin via inhibition of the SMAD signaling pathway in osteoblasts
Biochem Cell Biol. 2021 Mar 1. doi: 10.1139/bcb-2020-0347. Online ahead of print.ABSTRACTDuloxetine, a selective serotonin-norepinephrine reuptake inhibitor, is currently recommended to use in the treatment of chronic painful disorders such as fibromyalgia, chronic musculoskeletal pain and diabetic peripheral neuropathy. We previously demonstrated that bone morphogenetic protein-4 (BMP-4) stimulates osteoprotegerin (OPG) production in osteoblast-like MC3T3-E1 cells, and that p70 S6 kinase positively regulates the OPG synthesis. The present study is aimed to investigate the effect of duloxetine on BMP-4-stimulated OPG synth...
Source: Biochemistry and Cell Biology - March 1, 2021 Category: Biochemistry Authors: Junko Tachi Takashi Onuma Shinobu Yamaguchi Woo Kim Tomoyuki Hioki Rie Matsushima-Nishiwaki Kumiko Tanabe Haruhiko Tokuda Osamu Kozawa Hiroki Iida Source Type: research

Duloxetine suppresses BMP-4-induced release of osteoprotegerin via inhibition of the SMAD signaling pathway in osteoblasts
Biochem Cell Biol. 2021 Mar 1. doi: 10.1139/bcb-2020-0347. Online ahead of print.ABSTRACTDuloxetine, a selective serotonin-norepinephrine reuptake inhibitor, is currently recommended to use in the treatment of chronic painful disorders such as fibromyalgia, chronic musculoskeletal pain and diabetic peripheral neuropathy. We previously demonstrated that bone morphogenetic protein-4 (BMP-4) stimulates osteoprotegerin (OPG) production in osteoblast-like MC3T3-E1 cells, and that p70 S6 kinase positively regulates the OPG synthesis. The present study is aimed to investigate the effect of duloxetine on BMP-4-stimulated OPG synth...
Source: Biochemistry and Cell Biology - March 1, 2021 Category: Biochemistry Authors: Junko Tachi Takashi Onuma Shinobu Yamaguchi Woo Kim Tomoyuki Hioki Rie Matsushima-Nishiwaki Kumiko Tanabe Haruhiko Tokuda Osamu Kozawa Hiroki Iida Source Type: research

DUSP4 inhibits autophagic cell death in PTC by inhibiting JNK-BCL2-Beclin1 signaling
This study aimed to explore the relationship between DUSP4 and JNK-mediated autophagic cell death in PTC. In this study, we explored the roles of DUSP4 in PTC using gain-of-function and loss-of-function assays. In addition, we further identified the significance of JNK-BCL2-Beclin1-autophagy signaling on DUSP4-regulated PTC carcinogenesis by combining DUSP4 silencing with JNK specific inhibitor (SP600125). We found that DUSP4 silencing promoted the phosphorylation of JNK and BCL2 in PTC cells and enhanced the release of Beclin1 from BCL2-Beclin1 complex. DUSP4 silencing promoted autophagy and death in PTC cells.The death a...
Source: Biochemistry and Cell Biology - February 23, 2021 Category: Biochemistry Authors: Huixiang He Zhenshuang Du Jianqing Lin Wenyi Wu Yihuang Yu Source Type: research

DUSP4 inhibits autophagic cell death in PTC by inhibiting JNK-BCL2-Beclin1 signaling
This study aimed to explore the relationship between DUSP4 and JNK-mediated autophagic cell death in PTC. In this study, we explored the roles of DUSP4 in PTC using gain-of-function and loss-of-function assays. In addition, we further identified the significance of JNK-BCL2-Beclin1-autophagy signaling on DUSP4-regulated PTC carcinogenesis by combining DUSP4 silencing with JNK specific inhibitor (SP600125). We found that DUSP4 silencing promoted the phosphorylation of JNK and BCL2 in PTC cells and enhanced the release of Beclin1 from BCL2-Beclin1 complex. DUSP4 silencing promoted autophagy and death in PTC cells.The death a...
Source: Biochemistry and Cell Biology - February 23, 2021 Category: Biochemistry Authors: Huixiang He Zhenshuang Du Jianqing Lin Wenyi Wu Yihuang Yu Source Type: research

DUSP4 inhibits autophagic cell death in PTC by inhibiting JNK-BCL2-Beclin1 signaling
This study aimed to explore the relationship between DUSP4 and JNK-mediated autophagic cell death in PTC. In this study, we explored the roles of DUSP4 in PTC using gain-of-function and loss-of-function assays. In addition, we further identified the significance of JNK-BCL2-Beclin1-autophagy signaling on DUSP4-regulated PTC carcinogenesis by combining DUSP4 silencing with JNK specific inhibitor (SP600125). We found that DUSP4 silencing promoted the phosphorylation of JNK and BCL2 in PTC cells and enhanced the release of Beclin1 from BCL2-Beclin1 complex. DUSP4 silencing promoted autophagy and death in PTC cells.The death a...
Source: Biochemistry and Cell Biology - February 23, 2021 Category: Biochemistry Authors: Huixiang He Zhenshuang Du Jianqing Lin Wenyi Wu Yihuang Yu Source Type: research

DUSP4 inhibits autophagic cell death in PTC by inhibiting JNK-BCL2-Beclin1 signaling
This study aimed to explore the relationship between DUSP4 and JNK-mediated autophagic cell death in PTC. In this study, we explored the roles of DUSP4 in PTC using gain-of-function and loss-of-function assays. In addition, we further identified the significance of JNK-BCL2-Beclin1-autophagy signaling on DUSP4-regulated PTC carcinogenesis by combining DUSP4 silencing with JNK specific inhibitor (SP600125). We found that DUSP4 silencing promoted the phosphorylation of JNK and BCL2 in PTC cells and enhanced the release of Beclin1 from BCL2-Beclin1 complex. DUSP4 silencing promoted autophagy and death in PTC cells.The death a...
Source: Biochemistry and Cell Biology - February 23, 2021 Category: Biochemistry Authors: Huixiang He Zhenshuang Du Jianqing Lin Wenyi Wu Yihuang Yu Source Type: research

DUSP4 inhibits autophagic cell death in PTC by inhibiting JNK-BCL2-Beclin1 signaling
This study aimed to explore the relationship between DUSP4 and JNK-mediated autophagic cell death in PTC. In this study, we explored the roles of DUSP4 in PTC using gain-of-function and loss-of-function assays. In addition, we further identified the significance of JNK-BCL2-Beclin1-autophagy signaling on DUSP4-regulated PTC carcinogenesis by combining DUSP4 silencing with JNK specific inhibitor (SP600125). We found that DUSP4 silencing promoted the phosphorylation of JNK and BCL2 in PTC cells and enhanced the release of Beclin1 from BCL2-Beclin1 complex. DUSP4 silencing promoted autophagy and death in PTC cells.The death a...
Source: Biochemistry and Cell Biology - February 23, 2021 Category: Biochemistry Authors: Huixiang He Zhenshuang Du Jianqing Lin Wenyi Wu Yihuang Yu Source Type: research

Lactoferrin extends its reach into South America
Biochem Cell Biol. 2021 Feb;99(1):v-vii. doi: 10.1139/bcb-2021-0025.NO ABSTRACTPMID:33617378 | DOI:10.1139/bcb-2021-0025 (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - February 22, 2021 Category: Biochemistry Authors: Theresa J Ochoa Hans J Vogel Source Type: research

Predicting human RNA quadruplex helicases through comparative sequence approaches and helicase mRNA interactome analyses
Biochem Cell Biol. 2021 Feb 15. doi: 10.1139/bcb-2020-0590. Online ahead of print.ABSTRACTRNA quadruplexes are non-canonical nucleic acid structures involved in several human disease states and are regulated by a specific subset of RNA helicases. Given the difficulty in identifying RNA quadruplex helicases due to the multifunctionality of these enzymes, we sought to provide a comprehensive in silico analysis of features found in validated RNA quadruplex helicases to predict novel human RNA quadruplex helicases. Using the 64 human RNA helicases, we correlated their amino acid compositions with subsets of RNA quadruplex heli...
Source: Biochemistry and Cell Biology - February 15, 2021 Category: Biochemistry Authors: Steven J Dupas Daniel Gussakovsky Alvan Wai Mira J F Brown Georg Hausner Sean A McKenna Source Type: research

Predicting human RNA quadruplex helicases through comparative sequence approaches and helicase mRNA interactome analyses.
Abstract RNA quadruplexes are non-canonical nucleic acid structures involved in several human disease states and are regulated by a specific subset of RNA helicases. Given the difficulty in identifying RNA quadruplex helicases due to the multifunctionality of these enzymes, we sought to provide a comprehensive in silico analysis of features found in validated RNA quadruplex helicases to predict novel human RNA quadruplex helicases. Using the 64 human RNA helicases, we correlated their amino acid compositions with subsets of RNA quadruplex helicases categorized by varying level of evidence of RNA quadruplex interac...
Source: Biochemistry and Cell Biology - February 15, 2021 Category: Biochemistry Authors: Dupas SJ, Gussakovsky D, Wai A, Brown MJF, Hausner G, McKenna SA Tags: Biochem Cell Biol Source Type: research

LFchimera: a synthetic mimic of the two antimicrobial domains of bovine lactoferrin.
Abstract Saliva is essential for the maintenance of oral health. When salivary flow is impaired, the risk of various oral diseases such as caries and candidiasis increases drastically. Under healthy conditions, saliva provides effective protection against microbial colonization by the collaborative action of numerous host-defense molecules. This review describes how saliva has been the guideline for the design and characterization of a heterodimeric antimicrobial construct called LFchimera. This construct mimics the helical parts of two antimicrobial domains in the crystal structure of bovine lactoferrin. It shows...
Source: Biochemistry and Cell Biology - February 9, 2021 Category: Biochemistry Authors: Ligtenberg AJM, Bikker FJ, Bolscher JGM Tags: Biochem Cell Biol Source Type: research

Cetrimonium bromide promotes lipid clearance via TFEB-mediated autophagy-lysosome activation in hepatic cells.
Abstract Autophagy plays a key role in the metabolism of macromolecules by the lysosomal degradative machinery. The transcription factor EB (TFEB) regulates autophagosome biogenesis and lysosome function, and promoting TFEB activity has emerged as a potential strategy for the treatment of metabolic disorders. Here, we describe that cetrimonium bromide (CTAB), a quaternary ammonium compound, promotes autophagy and lysosomal biogenesis by inducing the nuclear translocation of TFEB in hepatic cells. shRNA-mediated TFEB knockdown inhibits CTAB-induced autophagy and lysosomal biogenesis. Mechanistically, CTAB treatment...
Source: Biochemistry and Cell Biology - February 9, 2021 Category: Biochemistry Authors: Liu Z, Wang X, Shi Z, Xu J, Lin J, Li D, Zhang X, Li Y, Zhao Q, Tao L, Diao A Tags: Biochem Cell Biol Source Type: research

The endless battle between phages and CRISPR-Cas systems in Streptococcus thermophilus.
Abstract This review describes the contribution of basic research on phage-bacteria interactions to the understanding of CRISPR-Cas systems as well as its various applications. It focuses on the natural function of CRISPR-Cas system as an adaptive defense mechanism against mobile genetic elements such as bacteriophage genomes and plasmids. Some of the advances in the characterization of the type II-A CRISPR-Cas system of Streptococcus thermophilus and Streptococcus pyogenes later led to the development of the CRISPR-Cas9 genome editing technology. We mostly discuss the three stages of the CRISPR-Cas system in S. t...
Source: Biochemistry and Cell Biology - February 3, 2021 Category: Biochemistry Authors: Philippe C, Moineau S Tags: Biochem Cell Biol Source Type: research

E3 ubiquitin ligase HECW1 promotes the metastasis of non-small cell lung cancer cells through mediating the ubiquitination of Smad4.
Abstract Lung cancer is the leading cause of cancer-related death globally. Ubiquitin modification plays a crucial role in regulation of gene expression, and is closely related to cancer pathogenesis. The aim of our study is to demystify the role and action mechanism of HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1) in non-small cell lung cancer (NSCLC). Here, we demonstrated that HECW1 expression was notably increased in NSCLC cell lines and tissues. Increasing of HECW1 markedly enhanced, whereas decreasing of HECW1 notably inhibited the proliferation of NSCLC cells. Moreover, the express...
Source: Biochemistry and Cell Biology - February 2, 2021 Category: Biochemistry Authors: Lu C, Ning G, Si P, Zhang C, Liu W, Ge W, Cui K, Zhang R, Ge S Tags: Biochem Cell Biol Source Type: research

G protein Coupled Receptor Kinase 5 Modifies the Nucleolar Stress Response Activated by Actinomycin D.
In this report, we show that GRK5 modifies the nucleolar stress response activated by the DNA polymerase inhibitor, Actinomycin D (ActD). We showed an increased sensitivity to the apoptotic effects of ActD on cervical HeLa and the breast cancer cell line MDA MB 231 with reduced GRK5 protein expressions. Furthermore, the rate of response to ActD varied between the two types of breast cancer cells tested, MDA MB 231 and MCF7 cells, since they have innate differences in GRK5 protein expressions. During the early ActD treatment period, GRK5 phosphorylates nucleophosmin (NPM1) at T199 before and during treatment. Phosphorylatio...
Source: Biochemistry and Cell Biology - January 28, 2021 Category: Biochemistry Authors: Sok V, Jacinto AZ, Peng N, Eldemerdash M, Le L, Tran PD, Feng LF, Patel JR, Gi M, Ammon JC, So CH Tags: Biochem Cell Biol Source Type: research

EGFR activity upregulates lactate dehydrogenase A (LDHA) expression, LDH activity and lactate secretion in cultured IB3-1 cystic fibrosis lung epithelial cells.
ta-Coloma T Abstract Cystic fibrosis (CF) is caused by mutations in the CFTR gene. It has been postulated that a reduced HCO3- transport in CF through CFTR may lead to a decreased airway surface liquid (ASL) pH. In contrast, others have reported no changes in the extracellular pH (pHe). We have recently reported that in carcinoma Caco-2/pRS26 cells (shRNA for CFTR) or CF lung epithelial IB3-1 cells, the CFTR failure decreased mitochondrial Complex I activity due to an autocrine IL1B loop, and increased lactic acid production. The secreted lactate accounted for the reduced pHe since oxamate fully restored the pHe. ...
Source: Biochemistry and Cell Biology - January 22, 2021 Category: Biochemistry Authors: Massip-Copiz MM, Valdivieso ÁG, Clauzure M, Mori C, Asensio CJA, Aguilar MÁ, Santa-Coloma T Tags: Biochem Cell Biol Source Type: research

Role of SIRT2 in regulating the dexamethasone-activated autophagy pathway in skeletal muscle atrophy.
Abstract The proteolytic autophagy system is involved in a major regulatory pathway in dexamethasone (Dex)-induced muscle atrophy. Sirtuin 2 (SIRT2) is known to participate in modulating autophagy signaling, exerting effects in skeletal muscle atrophy. We aimed to determine the effects of SIRT2 on autophagy in Dex-induced myoatrophy. Mice were randomly divided into the normal, Dex, and sirtinol groups. C2C12 cells were differentiated into myotubes and transfected with short hairpin (sh)-Sirt2-green fluorescent protein (GFP) or Sirt2-GFP lentivirus. To evaluate the mass and function of skeletal muscles, we measured...
Source: Biochemistry and Cell Biology - January 22, 2021 Category: Biochemistry Authors: Han Z, Chang C, Zhu W, Zhang Y, Zheng J, Kang X, Jin G, Gong Z Tags: Biochem Cell Biol Source Type: research

Lactoferrin receptors in Gram-negative bacteria: an evolutionary perspective.
Abstract In this short review, we outline the major events that led to the development of iron acquisition systems in Gram-negative bacteria and mammals since the beginning of life on earth. Naturally, the interaction between these organisms led to the development of a wonderfully complex set of protein systems used for competition over a once prevalent (but no longer) biocatalytic cofactor. These events led to the appearance of the lactoferrin gene, which has since been exploited into adopting countless new functions, including the provision of highly bactericidal degradation products. In parallel to lactoferrin'...
Source: Biochemistry and Cell Biology - January 19, 2021 Category: Biochemistry Authors: Ostan NKH, Moraes TF, Schryvers AB Tags: Biochem Cell Biol Source Type: research

EDTA as a chelating agent in quantitative 1H-NMR of biologically important ions.
Abstract Biologically important ions (Ca, K, Mg, Fe, and Zn) play major roles in numerous biological processes, with their homeostatic balance being necessary for the maintenance of cellular mechanisms. Sudden and severe loss in homeostasis of just one biologically important ion can cause cascading negative effects. Being able to quickly, accurately and reliably quantify biologically important ions in human bio-fluid samples is something that has been sorely lacking within the field of metabolomics. 1H-NMR is a well-known analytical platform in metabolomics but ions are invisible on 1H-NMR spectra. The foundation ...
Source: Biochemistry and Cell Biology - January 15, 2021 Category: Biochemistry Authors: Mathuthu E, Janse van Rensburg A, Du Plessis D, Mason S Tags: Biochem Cell Biol Source Type: research

Preventive Cancer Vaccination by P5 HER-2/neo Derived Peptide-pulsed Peripheral Blood Mononuclear Cells in Mouse Model of Breast Cancer.
This study aimed to compare the prophylactic effects of dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) based vaccines by pulsing them in vitro with p5 peptide. The different groups of mice were injected by free peptide or peptide pulsed with DCs or PBMCs. Two weeks after the last boosting dose, immunological tests were performed on splenocyte suspensions of three mice, and the remaining mice in each group were evaluated for tumor growth and survival. The IFN-γ, granzyme B, and IL-10 were detected in T cells. Additionally, IFN-γ and perforin as well as mRNA levels of some genes associated w...
Source: Biochemistry and Cell Biology - January 4, 2021 Category: Biochemistry Authors: Dehghan-Manshadi M, Nikpoor AR, Hadinedoushan H, Zare F, Sankian M, Fesahat F, Tahoori MT, Jaafari MR, Rafatpanah H Tags: Biochem Cell Biol Source Type: research

Dystonin loss-of-function leads to impaired autophagy-endolysosomal pathway dynamics.
Abstract The neuronal dystonin protein (DST-a) is a large cytoskeletal linker important for integrating the various components of the cytoskeleton. Recessive Dst mutations lead to a sensory neuropathy in mice known as dystonia musculorum (Dstdt). The disease is characterized by ataxia, autonomic disturbances, and ultimately death, which are associated with massive dorsal root ganglion (DRG) sensory neuron degeneration. Recent investigation of Dstdt sensory neurons revealed an accumulation in autophagosomes and a disruption in autophagic flux, which was believed to be due to insufficient motor protein availability....
Source: Biochemistry and Cell Biology - December 21, 2020 Category: Biochemistry Authors: Lynch-Godrei A, De Repentigny Y, Ferrier A, Gagnon S, Kothary R Tags: Biochem Cell Biol Source Type: research

Metastatic TNBC is highly associated with a fused mitochondrial morphology and a glycolytic and lipogenic metabolism programmation.
rtiz R, García N Abstract Mitochondria modify their function and morphology to satisfice the bioenergetic demand of the cells. Cancer cells take advantage of these features to sustain their metabolic, proliferative, metastatic, and survival necessities. Therefore, the understanding of mitochondrial morphologic changes of the different grades of Triple-Negative Breast Cancer (TNBC) could be relevant for the design of novel treatments. Consequently, this research aimed to explore the mitochondria morphology and gene expression of some proteins related to mitochondrial dynamics as well as proteins related to o...
Source: Biochemistry and Cell Biology - December 20, 2020 Category: Biochemistry Authors: Pérez-Treviño P, Aguayo-Millán CD, Santuario-Facio SK, Vela-Guajardo JE, Salazar E, Camacho-Morales A, Ortiz R, García N Tags: Biochem Cell Biol Source Type: research

Melatonin Inhibits Vascular Endothelial Cell Pyroptosis by Improving Mitochondrial Function via UQCRC1 Up-regulation and Demethylation.
In this study, melatonin attenuated the expression of pyroptosis-related genes, including NLRP3, caspase-1 and IL-1β, in human umbilical vein endothelial cells treated with oxidised low-density lipoprotein. Furthermore, melatonin up-regulated ten-eleven translocation 2 (TET2) expression, inhibited ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) methylation and reduced pyroptosis. The up-regulation of UQCRC1 via melatonin improved mitochondrial function, thereby inhibiting oxidative stress and endothelial cell pyroptosis. Collectively, our results indicated that melatonin prevented endothelial cell pyroptosis ...
Source: Biochemistry and Cell Biology - December 17, 2020 Category: Biochemistry Authors: Zeng J, Tao J, Xia L, Zeng Z, Chen J, Wang Z, Meng J, Liu L Tags: Biochem Cell Biol Source Type: research

Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines.
In this study, we explored the effect of bLF, LFchimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay; cell morphological changes and damage were determined by Giemsa and phalloidin-TRITC and DAPI staining; and apoptotic and necrotic cells were identified by Alexa Fluor® 488 Annexin V and PI staining. Additionally, the effect of combinations of bLF and LF-peptides with cisplatin was assessed. BLF and LF-peptides inhibited the proliferation of uterine cancer cells an...
Source: Biochemistry and Cell Biology - December 12, 2020 Category: Biochemistry Authors: Ramírez-Sánchez DA, Arredondo-Beltran IG, Canizalez-Roman A, Flores-Villaseñor H, Nazmi K, Bolscher JGM, Leon-Sicairos N Tags: Biochem Cell Biol Source Type: research

TFAP2A inhibits microRNA-126 expression at a transcriptional level to aggravate ischemic neuronal injury.
This study was performed to investigate the effect of transcription factor AP-2 alpha (TFAP2A) and the underlying mechanism in oxygen-glucose deprivation (OGD) cell model and transient global cerebral ischemia (tGCI) rat model. Based on CCK-8 and Hoechst staining results, silencing of TFAP2A could enhance the viability of OGD-treated PC12 cells and decrease the apoptotic rate of cells. ChIP assay was performed to detect the binding of TFAP2A to the promoter region of microRNA (miR)-126, and we found that TFAP2A could inhibit miR-126 expression. Further mechanistic investigation showed that miR-126 targeted polo like kinase...
Source: Biochemistry and Cell Biology - December 2, 2020 Category: Biochemistry Authors: Gao Z, Zhang J, Wu Y Tags: Biochem Cell Biol Source Type: research

SGLT1 knockdown prevents glucose fluctuation-induced apoptosis of cardiomyocytes through attenuating oxidative stress and mitochondrial dysfunction.
Abstract Blood glucose fluctuation has been validated to be more detrimental than constant high glucose in the development of cardiovascular complications of diabetes mellitus (DM). Sodium‑glucose cotransporter 2 (SGLT2) inhibitors have been developed as antidiabetic drugs with cardiovascular benefits. However, whether inhibition of SGLT1 protects the diabetic heart remains to be elucidated. The present study investigated the role of SGLT1 in rat H9c2 cardiomyocytes subjected to glucose fluctuation and the underlying mechanisms. The results indicated that SGLT1 knockdown was able to restore cell proliferation an...
Source: Biochemistry and Cell Biology - December 1, 2020 Category: Biochemistry Authors: Chai Q, Miao J, Liu M, Zhang Z, Meng Z, Wu W Tags: Biochem Cell Biol Source Type: research

The mechanism of BAF60c in myocardial metabolism through the PGC1 α/PPARα/mTOR signaling pathway in rats with heart failure.
This study explored the mechanism of BAF60c in heart failure (HF). Fetal/adult rat models of HF were established, and the levels of cardiac contractile proteins and energy metabolism-, oxidative metabolism- and glycolysis-related factors were detected. Overexpression/siRNA BAF60c plasmids were injected into adult HF rats to estimate myocardial glucose uptake, high-energy phosphate contents, mitochondrial function, and cell proliferation and apoptosis. The overexpression/siRNA BAF60c plasmids were transfected into cardiac hypertrophic H9C2 cells to explore the in vitro effects. The interaction of BAF60c and PGC1α was ...
Source: Biochemistry and Cell Biology - November 27, 2020 Category: Biochemistry Authors: Chen Q, Chen L, Jian J, Li J, Zhang X Tags: Biochem Cell Biol Source Type: research

Long-term treatment with insulin and retinoic acid increased glucose utilization in L6 muscle cells via glycogenesis.
Abstract The skeletal muscle regulates glucose homeostasis. Here, the effects of vitamin A metabolites including retinoic acid (RA) alone, and in combination with insulin, on glucose utilization were investigated in rat L6 muscle cells during the differentiation process. L6 cells were treated with differentiation medium containing retinol, retinal, RA, and (or) insulin. The glucose levels and pH values in the medium were measured every 2 days. The expression levels of insulin signaling and glycogen synthesis proteins, as well as glycogen content were determined. Retinal and RA reduced the glucose content and pH le...
Source: Biochemistry and Cell Biology - November 20, 2020 Category: Biochemistry Authors: Goff M, Chen G Tags: Biochem Cell Biol Source Type: research

Long noncoding RNA RP11-909N17.2 promotes proliferation, invasion, and migration of hepatocellular carcinoma by regulating microRNA-767-3p.
Abstract Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related deaths worldwide, especially in developing countries. Although advances in surgical procedures and targeted medicine have improved the overall survival of patients with HCC, the prognosis is poor. Hence, there is a need to identify novel therapeutic targets for HCC. Here, we report that the expression of RP11-909N17.2, a novel, long, noncoding RNA (lncRNA), is dysregulated in patients with HCC and cell lines. Additionally, this study demonstrated that RP11-909N17.2 facilitates the proliferation and invasion of HCC cells by b...
Source: Biochemistry and Cell Biology - November 19, 2020 Category: Biochemistry Authors: Liu Q, Dai SJ, Dong L, Li H Tags: Biochem Cell Biol Source Type: research

Amplified LncRNA PVT1 promotes lung cancer proliferation and metastasis by facilitating VEGFC expression.
Abstract Although the abundance of long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) in lung cancer has been well researched, the underlying mechanisms behind its effects were unknown. Here we investigated the molecular events regulating PVT1 in lung cancer. The pro-proliferative property of PVT1 was examined using a xenograft tumor model. Transwell chambers were used to analyze the impact of PVT1 expression on cell invasiveness and migration. In vivo metastasis was examined by tail-vein-injection in mice. Direct binding of miR-128 to PVT1 was investigated using a probe pulldown assay. The r...
Source: Biochemistry and Cell Biology - November 9, 2020 Category: Biochemistry Authors: Pan Y, Liu L, Cheng Y, Yu J, Feng Y Tags: Biochem Cell Biol Source Type: research

lncRNA CTBP1-AS2 promotes proliferation and migration of glioma by modulating miR-370-3p-Wnt7a-mediated epithelial-mesenchymal transition.
This study provides new insights that a novel lncRNA, CTBP1-AS2, regulates EMT of glioma by modulating the miR-370-3p-Wnt7a axis. PMID: 33150795 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 5, 2020 Category: Biochemistry Authors: Li Y, Zong J, Zhao C Tags: Biochem Cell Biol Source Type: research

Silencing linc00662 inhibits cell proliferation and colony formation of lung cancer cells via regulating miR-145-5p/PAFAH1B2 axis.
Abstract Lung cancer is the most common cause of cancer-related death in the world. Long non-coding RNAs (lncRNAs) are longer than 200 nucleotides transcripts which are not translated into protein. Linc00662 is overexpressed in lung cancer. However, the roles of linc00662 involved in lung cancer progression are still unknown. In our study, we found that linc00662 was overexpressed in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) by analyzing the TCGA data. We knockdown the linc00662 expression using siRNAs and found that silencing linc00662 significantly inhibited the proliferation and colony ...
Source: Biochemistry and Cell Biology - October 27, 2020 Category: Biochemistry Authors: Xu ZY, Peng J, Shi ZZ, Chen XL, Cheng HZ, Wang H, Wang Y, Wang GP, Jiang W, Peng H Tags: Biochem Cell Biol Source Type: research

Thidiazuron suppresses breast cancer progression via targeting miR-132 and miR-202-5p/PTEN axis mediated dysregulation of PI3K/AKT signaling pathway.
Abstract Chemo-resistance and metastatic disease development are the most common causes of breast cancer recurrence and death. Thidiazuron (TDZ) is a plant growth regulator, its biological role on human and animals has not been yet clarified. In the present study, we investigated the anticancer activity of this plant phytohormone on the drug resistant-triple negative breast cancer MDA-MB-231 cell line. Treatment of the breast cancer cells with TDZ (1-50 μM) caused more stressful environment and induced a significant increase in percentages of active caspases positive cells. In addition, TDZ treatment (5 and 10 ...
Source: Biochemistry and Cell Biology - October 24, 2020 Category: Biochemistry Authors: Ibrahim HI, Ismail MB, Ammar RB, Ahmed E Tags: Biochem Cell Biol Source Type: research

Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKC δ-NLRC4 axis.
Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKCδ-NLRC4 axis. Biochem Cell Biol. 2020 Oct 16;: Authors: Yang F, Yang Y, Zeng W Abstract Anti-double-stranded DNA (anti-dsDNA) has been identified to be closely related to brain inflammatory burden after ischemic stroke. Here, we studied the inflammatory cascade after dsDNA and investigated the mechanisms of its pro-inflammatory role in systemic lupus erythematosus (SLE). The IL-1β and IL-6 levels in serum of SLE patients and controls were evaluate by ELISA, and the caspas...
Source: Biochemistry and Cell Biology - October 16, 2020 Category: Biochemistry Authors: Yang F, Yang Y, Zeng W Tags: Biochem Cell Biol Source Type: research

miR-138-5p knockdown promotes osteogenic differentiation through FOXC1 up-regulation in human bone mesenchymal stem cells.
This study aimed to certify the hypothesis that miR-138-5p is expected to reduced osteodifferentiation of human bone mesenchymal stem cells (hBMSCs) by FOXC1 down-regulation. hBMSCs were separated from bone marrow and osteogenic induction medium was added to stimulate osteogenic differentiation. Flow cytometric analysis was applied to evaluate the expression of cell surface antigens associated with hBMSCs, including CD29, CD44, CD90, CD45 and CD34. qRT-PCR assay and western blot assay were determined to measure the mRNA and protein expression of miR-138-5p, OCN, RUNX2, BSP, ALP and FOXC1. Alkaline phosphatase (ALP) stainin...
Source: Biochemistry and Cell Biology - October 15, 2020 Category: Biochemistry Authors: Zhang L, Liu Y, Feng B, Liu LG, Zhou YC, Tang H Tags: Biochem Cell Biol Source Type: research

Circ_0000043 promotes breast cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the miR-136/ Smad3 axis.
In conclusion, our study demonstrates that circ_0000043 can promote BC progression via regulating the miR-136/Smad3 axis. PMID: 33043682 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - October 11, 2020 Category: Biochemistry Authors: Leng X, Huang G, Ding J, Ma F Tags: Biochem Cell Biol Source Type: research

A Canadian perspective on severe acute respiratory syndrome coronavirus 2 infection and treatment: how prevalent underlying inflammatory disease contributes to pathogenesis.
Abstract The coronavirus disease 2019 (COVID-19), a serious respiratory illness caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has emerged as a global pandemic. Canada reported its first case of COVID-19 on 25th January 2020. By March 2020 the virus had spread within Canadian communities reaching the most frail and vulnerable elderly population in long-term care facilities. The majority of cases were reported in the provinces of Quebec, Ontario, Alberta and British Columbia and the highest mortality was seen among individuals aged 65 years or older. Canada has the highest prevalence an...
Source: Biochemistry and Cell Biology - October 7, 2020 Category: Biochemistry Authors: Willows SD, Alam SB, Sandhu JK, Kulka M Tags: Biochem Cell Biol Source Type: research

LncRNA IDH1-AS1 suppresses cell proliferation and tumor growth in glioma.
Abstract Glioma is a type of brain tumor that is common globally, and is associated with a variety of genetic changes. It has been reported that isocitrate dehydrogenase 1 (IDH1) is overexpressed in glioma and in HeLa cells. The lncRNA IDH1-AS1 is believed to interact with IDH1, and when IDH1-AS1 is overexpressed, HeLa cell proliferation is inhibited. However, the effects of IDH1-AS1 on glioma were relatively unknown. The results from this work show that IDH1-AS1 is downregulated in the glioma tissues. We used primary glioblastoma cell lines U251 and U87-MG to study the effects of IDH1-AS1 on glioma cell growth, i...
Source: Biochemistry and Cell Biology - September 28, 2020 Category: Biochemistry Authors: Wang J, Quan Y, Lv J, Dong Q, Gong S Tags: Biochem Cell Biol Source Type: research

The lncRNA TUG1 promotes cell growth and migration via the TUG1/miR-145-5p/TRPC6 pathway in colorectal cancer.
In conclusion, our study revealed a novel molecular mechanism of TUG1 in CRC progression and suggested the potential of the TUG1/miR-145-5p/TRPC6 pathway to serve as a target for the diagnosis and treatment of CRC. PMID: 32985219 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - September 26, 2020 Category: Biochemistry Authors: Wang X, Bai X, Yan Z, Guo X, Zhang Y Tags: Biochem Cell Biol Source Type: research

Upregulation of Bromodomain PHD ‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival.
Upregulation of Bromodomain PHD‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival. Biochem Cell Biol. 2020 Sep 27;: Authors: Miao J, Zhang M, Huang X, Xu L, Tang R, Wang H, Han S Abstract Bromodomain PHD finger transcription factor (BPTF) is a core subunit of nucleosome-remodeling factor (NURF) complex, which plays an important role in the development of several cancers. However, it is unknown whether BPTF regulates ovarian cancer (OC) progression. Relative expression of BPTF in cell lines and tissues of OC were measured by Western blot an...
Source: Biochemistry and Cell Biology - September 26, 2020 Category: Biochemistry Authors: Miao J, Zhang M, Huang X, Xu L, Tang R, Wang H, Han S Tags: Biochem Cell Biol Source Type: research

AC006262.5/miR-7855-5p/BPY2C axis facilitates hepatocellular carcinoma proliferation and migration.
Abstract Hepatocellular carcinoma (HCC) is a considerable threat to human life, and patients with HCC are usually diagnosed in the later stages. Although treatment for HCC has recently advanced rapidly, novel targets for HCC are still desperately needed, especially for precision medicine. Here, we identified an HCC enriched long non-coding RNA, AC006262.5, that promoted the proliferation, migration, and invasion of HCC both in vitro and in vivo. In addition, our results revealed that AC006262.5 bound to and regulated miR-7855-5p, a tumor suppressive miRNA in HCC. Moreover, our data illustrated that AC006262.5 regu...
Source: Biochemistry and Cell Biology - September 20, 2020 Category: Biochemistry Authors: Chen F, Wang Y, Cheng Y, Shi H, Li H, Jia M, Sha S, Dong L Tags: Biochem Cell Biol Source Type: research

Effect of bovine lactoferrin on prevention of late-onset sepsis in infants < 1500g: a pooled analysis of individual patient data from two randomized controlled trials.
Effect of bovine lactoferrin on prevention of late-onset sepsis in infants
Source: Biochemistry and Cell Biology - September 14, 2020 Category: Biochemistry Authors: Ochoa TJ, Loli S, Mendoza K, Carcamo C, Bellomo S, Cam L, Castañeda A, Campos M, Jacobs J, Cossey V, Zegarra J Tags: Biochem Cell Biol Source Type: research

Automated classification of mitotic catastrophe by use of the centromere fragmentation morphology.
We present an automated 2-step system for segmentation of cells exhibiting centromere fragmentation. The first step segments individual cells from clumps. We added two new terms, Weighted Local Repelling term (WLRt) and Weighted Gradient term (WGt), in the energy functional of the traditional Chan & Vese based level set method. WLRt was used to generate a repelling force when contours of adjacent cells merged and then penalized the overlap. WGt enhances gradients between overlapping cells. The second step consists of a new algorithm, SBaN (Shape-Based analysis of each nuclei), which extracts features like circularity, ...
Source: Biochemistry and Cell Biology - September 8, 2020 Category: Biochemistry Authors: Roy K, Lewis CW, Chan G, Bhattacharjee D Tags: Biochem Cell Biol Source Type: research

Effects of oral bovine lactoferrin on a mouse model of inflammation associated colon cancer.
Abstract Both ulcerative colitis and colonic Crohn's disease patients have a significantly increased risk of developing colorectal cancer. bLF is reported to inhibit the development of colon cancer in rats and mice, and in a placebo controlled trial, ingestion of bLF inhibited the growth of intestinal polyps. In addition, in a case study a Crohn's disease patient was reported to have remained in remission for over 7 years while ingesting 1 gram of bLF daily. Thus, bLF has an inhibitory effect on colon carcinogenesis, and it may also promote remission of Crohn's disease. The purpose of the present study was to begi...
Source: Biochemistry and Cell Biology - September 8, 2020 Category: Biochemistry Authors: Tanaka H, Gunasekaran S, Saleh DM, Alexander WT, Alexander DB, Ohara H, Tsuda H Tags: Biochem Cell Biol Source Type: research

Lactoferrin and hematoma detoxification after intracerebral hemorrhage.
Abstract This review discusses the role of lactoferrin (LTF) in detoxifying hematoma after intracerebral hemorrhage (ICH). Subsequent to ICH, neutrophils enter the ICH-affected brain, where they release various granule content, including LTF. LTF is an iron-binding glycoprotein that binds Fe3+ with high affinity. Unlike other iron binding proteins, LTF can retain Fe3+ at the low pH associated with inflamed tissue. LTF's ability to sequester Fe3+ is of particular importance to ICH pathogenesis, as large quantities of free iron, which is pro-oxidative and pro-inflammatory are generated in the ICH-affected brain due ...
Source: Biochemistry and Cell Biology - September 3, 2020 Category: Biochemistry Authors: Zhao X, Kruzel ML, Aronowski J Tags: Biochem Cell Biol Source Type: research

Antibiofilm activity of lactoferrin-derived synthetic peptides against Pseudomonas aeruginosa PAO1.
Abstract Many pathogenic bacteria can protect themselves from the effects of antibiotics and the host immune response system by forming biofilms. Eradication of bacterial biofilms typically requires much higher concentrations of antibiotics, than are normally needed to kill cultured planktonic cells, raising serious clinical concerns. In an attempt to try to prevent the formation of biofilms or to break up existing biofilms of pathogenic bacteria, herein we have used the standard crystal violet assay as well as the Calgary biofilm device, to test several lactoferrin- and lactoferricin-derived antimicrobial peptide...
Source: Biochemistry and Cell Biology - August 31, 2020 Category: Biochemistry Authors: Ramamourthy G, Vogel HJ Tags: Biochem Cell Biol Source Type: research

Transcriptional regulation of human abraxas brother protein 1 expression by yin yang 1.
In this study, we identified and characterized the core regulatory elements of the human ABRO1 gene and mapped them to the ABRO1 promoter region. Additionally, 5' rapid amplification of cDNA ends revealed that the transcriptional start site (TSS) was located -13 bp upstream from the start codon. Reporter gene, chromatin immunoprecipitation, and electrophoretic mobility shift assays demonstrated that ABRO1 transcription was regulated through cis-acting elements located in the region -89 to -59 bp upstream of the ABRO1 TSS and that these elements were targeted by yin yang 1 transcription factor (YY1). Moreover, YY1 overexpre...
Source: Biochemistry and Cell Biology - August 25, 2020 Category: Biochemistry Authors: Song P, Hong J, Wang Y, Yao X, Zhan Y, Yin R, Yu M, Li C, Yang X, Ge C Tags: Biochem Cell Biol Source Type: research

Dose Escalation Study of Bovine Lactoferrin in Preterm Infants: Getting the Dose Right.
Abstract Lactoferrin as a nutritional enteral supplement has emerged as a novel preventative therapy against serious infections in preterm infants, though neonatal studies have demonstrated variable results in part due to the lack of pharmacokinetic data and differences in products. We conducted a prospective, dose escalation (100, 200, and 300 mg/kg/day) safety study of bovine lactoferrin (Glanbia Nutritionals, USA) dissolved in sterile water (100 mg/ml) for 30 days in preterm infants
Source: Biochemistry and Cell Biology - August 25, 2020 Category: Biochemistry Authors: Kaufman DA, Berenz A, Itell HL, Conaway M, Blackman A, Nataro JP, Permar SR Tags: Biochem Cell Biol Source Type: research

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin.
Abstract Lactoferrin supplementation may help prevent infections in preterm infants but efficacy has varied with different dosing and products. We assessed the absorption and excretion of bovine lactoferrin (bLF) in thirty-one infants receiving 100, 200, or 300 mg/kg/day of enteral bLF for 30 days. BLF and human lactoferrin (hLF) in infant saliva, blood, urine, and stool and expressed or donor breast milk (EBM, DBM) collected before treatment initiation, study day 22, and one week after treatment cessation were measured by ELISA. During treatment, BLF was absorbed from the gastrointestinal tract, detected in plasm...
Source: Biochemistry and Cell Biology - August 24, 2020 Category: Biochemistry Authors: Itell HL, Berenz A, Mangan RJ, Permar SR, Kaufman DA Tags: Biochem Cell Biol Source Type: research