miR-1180 promotes cardiomyocyte cell cycle reentry after injury through the NKIRAS2-NF κB pathway.
miR-1180 promotes cardiomyocyte cell cycle reentry after injury through the NKIRAS2-NFκB pathway. Biochem Cell Biol. 2020 Jan 19;: Authors: Ding Y, Bi L, Wang J Abstract Heart failure (HF) is associated with considerable symptom burden and impairment for human health, including quality of life and physical functioning. Previous studies have indicated that miRNAs have important roles in regulating HF development. miR-1180 is influential in the proliferation, migration, invasion and chemoresistance of cancer cells; however, the underlying mechanism and role of miR‑1180 in cardiomyocytes remains ...
Source: Biochemistry and Cell Biology - January 19, 2020 Category: Biochemistry Authors: Ding Y, Bi L, Wang J Tags: Biochem Cell Biol Source Type: research

Protective effect of Hydralazine on a cellular model of Parkinson's disease: a possible role of hypoxia-inducible factor (HIF)-1 α.
Protective effect of Hydralazine on a cellular model of Parkinson's disease: a possible role of hypoxia-inducible factor (HIF)-1α. Biochem Cell Biol. 2020 Jan 15;: Authors: Mehrabani M, Nematollahi MH, Esmaili M, Bahrampour K, Abolhassani M, Sharifi AM, Pasban H, Saravani M, Mirzamohammadi S Abstract Parkinson's disease (PD) is a neurodegenerative disease accompanied by a low expression level of cerebral hypoxia-inducible factor (HIF-1α). Hence, activating the hypoxia-signaling pathway may be a favorable therapeutic approach for curing PD. The present study explored the efficacy of hydrala...
Source: Biochemistry and Cell Biology - January 15, 2020 Category: Biochemistry Authors: Mehrabani M, Nematollahi MH, Esmaili M, Bahrampour K, Abolhassani M, Sharifi AM, Pasban H, Saravani M, Mirzamohammadi S Tags: Biochem Cell Biol Source Type: research

Proceedings of the 14th annual RiboWest conference: perspectives and outcome.
Abstract The RiboWest Conference brings together RNA researchers in Canada with the 2-fold goals of fostering internationally competitive RNA research and of training the next generation of scientists. The 14th Annual RiboWest conference (RiboWest 2018) was held at the University of Lethbridge (Lethbridge, Alberta) from June 10th to 13th, 2018. This meeting was focused on all major aspects of RNA research, ranging from understanding the cellular role of RNA, studying RNA interactions and structures, and employing them as a therapeutic tool. The invited keynote speakers (5) provided insights into the wide-range of ...
Source: Biochemistry and Cell Biology - January 14, 2020 Category: Biochemistry Authors: Thakor N, Kothe U, Wieden HJ, Patel TR Tags: Biochem Cell Biol Source Type: research

Inhibition of brain-type glycogen phosphorylase ameliorates high glucose-induced cardiomyocyte apoptosis via Akt-HIF-1 α activation.
In conclusion, the present study suggested that inhibition of pygb prevents HG-induced cardiomyocyte apoptosis via Akt-HIF-α activation. PMID: 31905009 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - January 6, 2020 Category: Biochemistry Authors: Wu X, Huang W, Quan M, Chen Y, Tu J, Zhou J, Xin HB, Qian Y Tags: Biochem Cell Biol Source Type: research

miR-140-5p targeted FGF9 and inhibited the cell growth of laryngeal squamous cell carcinoma.
Abstract Increasing evidence has suggested that microRNAs (miRNAs) play critical roles in the initiation and development of cancers. Here, we found that miR-140-5p was significantly downregulated in both laryngeal squamous cell carcinoma (LSCC) tissues and cell lines. Decreased expression of miR-140-5p was significantly associated with the metastasis of LSCC. Overexpression of miR-140-5p inhibited proliferation and induced apoptosis of LSCC cells. Mechanistically, the fibroblast growth factor 9 (FGF9) was identified as the target of miR-140-5p. miR-140-5p bound the 3'-untranslated region (3'-UTR) of FGF9 and suppr...
Source: Biochemistry and Cell Biology - December 20, 2019 Category: Biochemistry Authors: Wang Y, Huang Q, Li F Tags: Biochem Cell Biol Source Type: research

Active Vitamin D activates chondrocyte autophagy to reduce osteoarthritis via mediating the AMPK/mTOR signaling pathway.
This study provided evidence that active VD might activate chondrocyte autophagy to reduce OA inflammation via activating the AMPK/mTOR signaling pathway. Active OA treatment might serve as a novel therapeutic option for OA treatment. PMID: 31815524 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - December 9, 2019 Category: Biochemistry Authors: Kong C, Wang C, Shi Y, Yan L, Xu J, Qi W Tags: Biochem Cell Biol Source Type: research

Glycerol kinase enhances hepatic lipid metabolism by repressing nuclear receptor subfamily 4 group A1 in the nucleus.
Abstract Glycerol kinase (GYK) plays a critical role in hepatic metabolism by converting glycerol to glycerol 3-phosphate in an ATP-dependent reaction. GYK isoform b is the only glycerol kinase present in whole cells and has a non-enzymatic moonlighting function in the nucleus. GYK isoform b acts as a co-regulator of nuclear receptor subfamily 4 group A1 (NR4A1) and participates in the regulation of hepatic glucose metabolism by protein-protein interaction with NR4A1. Herein, GYK expression was found to upregulate the expression of NR4A1-mediated lipid metabolism-related genes (SREBP1C, FASN, ACACA, and GPAM) in H...
Source: Biochemistry and Cell Biology - December 7, 2019 Category: Biochemistry Authors: Miao L, Su F, Yang Y, Liu Y, Wang L, Zhan Y, Yin R, Yu M, Li C, Yang X, Ge C Tags: Biochem Cell Biol Source Type: research

Circ_KATNAL1 regulates prostate cancer cell growth and invasion through miR-145-3p/WISP1 pathway.
Abstract Prostate cancer (PCa) is the second leading cause of death in men, and current studies have shown that circular RNAs (circRNAs) play important roles in its occurrence and development. Detection of circRNAs in PCa cells found that circ_KATNAL1 was down-regulated, mainly located in the cytoplasm and contained multiple binding sites of miR-145-3p, an anti-cancer miRNA. RIP detection with anti-AGO2 antibody, RNA pull down assay with biotin-labeled circ_KATNAL1 probe or miR-145-3p mimics, and dual luciferase reporter gene assay confirmed that circ_KATNAL1 directly bound to miR-145-3p in cells, and WISP1, which...
Source: Biochemistry and Cell Biology - December 4, 2019 Category: Biochemistry Authors: Zheng Y, Chen CJ, Lin ZY, Li JX, Liu J, Lin FJ, Zhou X Tags: Biochem Cell Biol Source Type: research

Homogeneity and heterogeneity of biological characteristics in mesenchymal stem cells from human umbilical cords and exfoliated deciduous teeth.
Abstract Mesenchymal stem cells (MSCs) have proven powerful potential for cell-based therapy both in regenerative medicine and disease treatment. Human umbilical cords and exfoliated deciduous teeth are the main sources to derive MSCs with nearly no donor injury and ethical issue. The goal of this study was to investigate the differences of biological characteristics in human umbilical cord mesenchymal stem cells (UCMSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). UCMSCs and SHEDs were identified by flow cytometry. The proliferation, differentiation, migration, chemotaxis, paracrine, immunomodul...
Source: Biochemistry and Cell Biology - December 3, 2019 Category: Biochemistry Authors: Yang C, Chen Y, Zhong L, You M, Yan Z, Luo M, Zhang B, Yang B, Chen Q Tags: Biochem Cell Biol Source Type: research

Omega-3 polyunsaturated fatty acids have beneficial effects on visceral fat in diet-induced obesity model.
In conclusion, omega-3 PUFAs have beneficial effects on the visceral fat of obese mice because they mitigate changes caused by the consumption of a high-fat diet. PMID: 31774300 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 27, 2019 Category: Biochemistry Authors: de Mello AH, Schraiber RB, Goldim MPS, Mathias K, Mendes C, Corrêa MEAB, Gomes ML, Silveira PCL, Schuck PF, Petronilho F, Rezin GT Tags: Biochem Cell Biol Source Type: research

Biochemistry and Cell Biology celebrates its 90th anniversary.
PMID: 31770000 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 26, 2019 Category: Biochemistry Authors: Davie J, Nelson C Tags: Biochem Cell Biol Source Type: research

IM-12 activates the Wnt- β-catenin signaling pathway and attenuates rtPA-induced hemorrhagic transformation in rats after acute ischemic stroke.
In this study, we used IM-12, an inhibitor of glycogen synthase kinase 3β (GSK-3β), to evaluate the role of the Wnt-β-catenin signaling pathway in recombinant tPA (rtPA)-induced HT. Sprague-Dawley rats were subjected to a middle cerebral artery occlusion (MCAO) model of ischemic stroke, and then were either administered rtPA, rtPA combined with IM-12, or the vehicle at 4 h after stroke was induced. Our results indicate that rats subjected to HT had more severe neurological deficits, brain edema, and blood-brain barrier (BBB) breakdown, and had a greater infarction volume than the control group. Rats treated ...
Source: Biochemistry and Cell Biology - November 26, 2019 Category: Biochemistry Authors: Wang T, Duan YM, Fu Q, Liu T, Yu JC, Sui ZY, Huang L, Wen GQ Tags: Biochem Cell Biol Source Type: research

MiR-539 inhibited the malignant behaviors of breast cancer cells by targeting SP1.
Abstract Aberrant expression of microRNAs (miRNAs) was involved in the initiation and progression of human cancers. Here, we found that miR-539 was down-regulated in breast cancer tissues and cell lines. Decreased expression of miR-539 was significantly associated with the lymph node metastasis of breast cancer patients. Overexpression of miR-539 inhibited the proliferation and promoted apoptosis of breast cancer cells. Moreover, highly expressed miR-539 significantly suppressed the epithelial mesenchymal transition (EMT) and sensitized cells to cisplatin treatment. Mechanistically, miR-539 was found to target the...
Source: Biochemistry and Cell Biology - November 19, 2019 Category: Biochemistry Authors: Cai F, Chen L, Sun Y, He C, Fu D, Tang J Tags: Biochem Cell Biol Source Type: research

PLOD2 promotes aerobic glycolysis and cell progression in colorectal cancer by upregulating HK2.
Abstract The purpose of the current study is to characterize the expression of procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), a membrane-bound homodimeric enzyme that specifically hydroxylates lysine in the telopeptide of procollagens, and assess the clinical significance of PLOD2 in colorectal cancer (CRC). Our results showed that PLOD2 was highly expressed in CRC tumor tissues samples and cell lines both in mRNA and protein level. Next, we found that PLOD2 was positively correlated with Grade (p=0.001), T stage (p=0.001), N stage (p
Source: Biochemistry and Cell Biology - November 19, 2019 Category: Biochemistry Authors: Du W, Liu N, Zhang Y, Liu X, Yang Y, Chen W, He Y Tags: Biochem Cell Biol Source Type: research

Nicotine-upregulated miR-30a arrests cell cycle in G1 phase by directly targeting CCNE2 in human periodontal ligament cells.
In conclusion, these findings indicate that nicotine-upregulated miR-30a inhibits the proliferation of human PDL cells by downregulating the expression of CCNE2. PMID: 31689122 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 5, 2019 Category: Biochemistry Authors: Wu L, Yang K, Gui Y, Wang X Tags: Biochem Cell Biol Source Type: research

Connecting proteins: shareable tools for reproducible interaction mapping.
Abstract A summary of the Jeanne Manery Fisher Memorial Lecture presented at the CSMB meeting in Montreal, June 2019. PMID: 31689129 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - November 5, 2019 Category: Biochemistry Authors: Gingras AC Tags: Biochem Cell Biol Source Type: research

Protective effect of nitronyl nitroxide against hypoxia induced damage in PC12 cells.
Abstract Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. 4'-hydroxyl-2-subsitiuted phenyl nitronyl nitroxide (HPN), a stable nitronyl nitroxide, shows excellent free radical scavenge activity. The purpose of this study was to investigate the protective effects of HPN on hypoxia-induced damage in PC12 cell. It was shown that HPN significantly attenuated hypoxia induced loss of cell viability, LDH release and morphological changes of PC12 cell. Moreover, hypoxic PC12 cells had an elevation in reactive oxygen species (ROS), malondialdehyde (MDA) and the expression of HIF-...
Source: Biochemistry and Cell Biology - November 5, 2019 Category: Biochemistry Authors: Luo H, Sun W, Shao J, Ma H, Jia Z, Jing L Tags: Biochem Cell Biol Source Type: research

PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination.
Abstract Promyelocytic leukemia nuclear bodies (PML NBs) are nuclear subdomains that respond to genotoxic stress by increasing in number via changes in chromatin structure. However, the role of the PML protein and PML NBs in specific mechanisms of DNA repair has not been fully characterized. Here, we have directly examined the role of PML in homologous recombination (HR) using I-SceI extrachromosomal and chromosome-based homology-directed repair (HDR) assays, and in HDR by CRISPR/Cas9-mediated gene editing. We determined that PML loss can inhibit HR in an extrachromosomal HDR assay but had less of an effect on CRI...
Source: Biochemistry and Cell Biology - October 31, 2019 Category: Biochemistry Authors: Attwood KM, Salsman J, Chung D, Mathavarajah S, Van Iderstine C, Dellaire G Tags: Biochem Cell Biol Source Type: research

Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumor stem cells (BTSCs).
Abstract Glioblastoma multiforme (GBM) is among the deadliest cancers, owing in part to complex inter- and intra-tumor heterogeneity and the presence of a population of stem-like cells called brain tumor stem cells (BTSCs/BTICs). These cancer stem cells survive treatment and confer resistance to the current therapies-namely, radiation and the chemotherapeutic, temozolomide (TMZ). TMZ induces cell death by alkylating DNA, and BTSCs resist this mechanism via a robust DNA damage response. Hence, recent studies aimed to sensitize BTSCs to TMZ using combination therapy, such as inhibition of DNA repair machinery. We ha...
Source: Biochemistry and Cell Biology - October 31, 2019 Category: Biochemistry Authors: Ross JA, Ahn BY, King J, Bressler KR, Senger DL, Thakor N Tags: Biochem Cell Biol Source Type: research

Long noncoding RNA HIF1A-AS2 facilitates cell survival and migration by sponging miR-33b-5p to modulate SIRT6 expression in osteosarcoma.
Abstract Long non-coding RNAs (lncRNAs) are emerging as vital regulators in various physiological and pathological processes. It was recently found that lncRNA HIF1A-AS2 could play oncogenic roles in several cancers. However, the function and regulatory mechanism of lncRNA HIF1A-AS2 in osteosarcoma (OS) remain largely unclear. In the present study, we demonstrated that HIF1A-AS2 was overexpressed in OS tissues and cells. HIF1A-AS2 downregulation could remarkably affect multiple OS cell biological functions, including cell proliferation, cell cycle progression, cell apoptosis, cell migration and cell invasion. Mech...
Source: Biochemistry and Cell Biology - October 18, 2019 Category: Biochemistry Authors: Lin H, Zhao Z, Hao Y, He J, He J Tags: Biochem Cell Biol Source Type: research

MiR-126 targets IL-17A to enhance proliferation and inhibit apoptosis in high-glucose-induced human retinal endothelial cells.
Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM), which results in vision loss. The present study aimed to explore the role of miR-126 in high-glucose-induced human retinal endothelial cells (HRECs) and its underlying molecular mechanism. The results showed that expression of miR-126 and interleukin-17A (IL-17A) in high-glucose-induced HRECs was downregulated and upregulated, respectively. Functionally, overexpression of miR-126 promoted proliferation and suppressed apoptosis in high-glucose-induced HRECs, while IL-17A reversed the effects induced by miR-126. However, overexpre...
Source: Biochemistry and Cell Biology - October 13, 2019 Category: Biochemistry Authors: Chen X, Yu X, Li X, Li L, Li F, Guo T, Guan C, Miao L, Cao G Tags: Biochem Cell Biol Source Type: research

Subcellular fractionation of frozen skeletal muscle samples.
Abstract Cell fractionation can be used to determine the localization and trafficking of proteins between cellular compartments such as cytosol, mitochondria and nuclei. Subcellular fractionation is usually performed immediately after tissue dissection since freezing may fragment cell membranes and induce organellar cross-contamination. Mitochondria are especially sensitive to freezing/thawing and mechanical homogenization. We proposed a protocol to improve soluble proteins retention in the mitochondrial fraction obtained from small amounts of frozen skeletal muscle. Fifty-milligram of red portion of gastrocnemius...
Source: Biochemistry and Cell Biology - October 12, 2019 Category: Biochemistry Authors: Firmino Dias PR, Gandra PG, Brenzikofer R, Macedo DV Tags: Biochem Cell Biol Source Type: research

MicroRNA-1298-5p inhibits cell proliferation and invasion of bladder cancer via downregulating connexin 43.
In this study, the results demonstrated that the expression levels of miR-1298 and Cx43 were significantly down-regulated and up-regulated in BC clinical tissues, respectively. The overexpression of miR-1298 inhibited cell proliferation, migration, and invasion in two BC cell lines via MTT assay, cell cycle assay, colony formation assay, transwell assay, gelatin zymography, and western blot, respectively. In addition, it was found that miR-1298 decreased Cx43 expression by directly targeting the 3'-UTR. Following, we observed that the promotion effect of Cx43 on BC cell proliferation, migration, and invasion could be parti...
Source: Biochemistry and Cell Biology - October 10, 2019 Category: Biochemistry Authors: Li G, Sun L, Mu Z, Liu S, Qu H, Xie Q, Hu B Tags: Biochem Cell Biol Source Type: research

Low n-6/n-3 PUFA ratio improves inflammation and myocardial ischemic reperfusion injury.
This study investigated the potential effect of n-6/n-3 polyunsaturated fatty acids (PUFA) on inflammation and myocardial ischemic reperfusion injury (MIRI) in rats, together with the underlying protective mechanisms, and screen out most effective ratio of n-6/n-3 within limits. The rats with pre-infarct treatment were distributed among 5 groups according to the n-6/n-3 ratio (36:1; 1:1, 5:1, 10:1, 50:1); for the post-infarct treatment, the rats were distributed among 6 groups, including the control group (36:1) which was subjected to a sham procedure; the model group (36:1); and 4 test groups (n-6/n-3 ratio: 1:1, 5:1, 10:...
Source: Biochemistry and Cell Biology - October 3, 2019 Category: Biochemistry Authors: Ma C, Xu Z, Lv H Tags: Biochem Cell Biol Source Type: research

Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells.
In this study, we found that extracellular PKM2 enhanced the migration of low-metastatic, benign colon cancer cells by upregulating claudin-1 expression and internalizing it to the cytoplasm and nucleus. Knock down of claudin-1 significantly reduced extracellular PKM2-induced cell migration. Inhibition of either protein kinase C (PKC) or epidermal growth factor receptor (EGFR) resulted in a reduction of extracellular PKM2 mediated claudin-1 expression, suggesting EGFR-PKC-claudin-1 as a signaling pathway in the extracellular PKM2-mediated tumorigenesis of colon cancer cells. PMID: 31545907 [PubMed - as supplied by pub...
Source: Biochemistry and Cell Biology - September 23, 2019 Category: Biochemistry Authors: Kim H, Kim SH, Hwang D, An J, Chung HS, Yang EG, Kim SY Tags: Biochem Cell Biol Source Type: research

MicroRNA-24 alleviates isoflurane-induced neurotoxicity in rat hippocampus via attenuation of oxidative stress.
This study was carried out to explore the biological roles of miR-24 in isoflurane-treated rat hippocampal neurons. Isoflurane-treated rat model was established to induce neurotoxicity. Gain- and loss- of function of miR-24 was performed and the size and Ca2+ permeability of mitochondria, cell proliferation and apoptosis and levels of oxidative stress-related factors were measured both in vivo and in vitro. Dual luciferase reporter gene assay was used to identify the target relation between miR-24 and p27kip1. In this study, isoflurane treatment decreased miR-24 expression, after which the neuronal apoptosis and the oxidat...
Source: Biochemistry and Cell Biology - September 18, 2019 Category: Biochemistry Authors: Li N, Yue L, Wang J, Wan Z, Bu W Tags: Biochem Cell Biol Source Type: research

MicroRNA profiling of human myeloid angiogenic cells derived from peripheral blood mononuclear cells.
In this study, we aimed to provide an up-to-date miRNA signature for human MACs. MACs were obtained by culture of human peripheral blood mononuclear cells in endothelial medium for 7 days. Using qPCR array analysis we identified 72 highly expressed miRNAs (Ct value
Source: Biochemistry and Cell Biology - September 4, 2019 Category: Biochemistry Authors: Zhang Q, Cannavicci A, Dai SC, Wang C, Kutryk MJ Tags: Biochem Cell Biol Source Type: research

Genes responsive to rapamycin and serum deprivation are clustered on chromosomes and undergo re-organization within local chromatin environments.
Abstract We previously demonstrated that genome reorganization, through chromosome territory repositioning, occurred concurrently with significant changes in gene expression in normal primary human fibroblasts treated with the drug rapamycin, or stimulated into quiescence. Although these events occurred concomitantly, it is unclear how specific changes in gene expression relate to reorganization of the genome at higher resolution. Using computational analyses, genome organization assays and microscopy, the relationship between chromosome territory positioning and gene expression was investigated. We determined tha...
Source: Biochemistry and Cell Biology - September 3, 2019 Category: Biochemistry Authors: Belak ZR, Pickering JA, Gillespie ZE, Audette GF, Eramian M, Mitchell JA, Bridger JM, Kusalik A, Eskiw CH Tags: Biochem Cell Biol Source Type: research

MiR-204 suppresses cell proliferation and promotes apoptosis in ovarian granulosa cells via targeting TPT1 in polycystic ovary syndrome.
Abstract MicroRNA (miR)-204 is known to be associated with several different diseases. Polycystic ovary syndrome (PCOS) has the highest incidence rate among the endocrine disorders in females between the ages of 18 and 44. We aimed to illustrate the miR-204 function in PCOS. MiR-204 expression levels in tissue and cell were examined through RT-qPCR. Colony formation assay and MTT assay were applied to detect the cell viability. Flow cytometry was employed to examine the apoptosis and cell cycle in cells. RNA binding protein immunoprecipitation assay and luciferase reporter assay were provided to demonstrate the di...
Source: Biochemistry and Cell Biology - August 28, 2019 Category: Biochemistry Authors: Sun X, Su S, Zhang G, Zhang H, Yu X Tags: Biochem Cell Biol Source Type: research

Silencing LINC00511 inhibits cell proliferation, migration and EMT via PTEN/AKT/FOXO1 signaling pathway in lung cancer.
Abstract Lung cancer is the most common cause of cancer-related death worldwide. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotide transcripts and are not translated into proteins. Increasing evidence has shown that lncRNAs are associated with several biological processes in cancer. However, the roles of LINC00511 in lung cancer progression remain unknown. In the present study, we confirmed that LINC00511 knockdown significantly inhibited cell proliferation and migration in A549, SPCA1 and H460 cells. Western blot results showed that silencing LINC00511 inhibited epithelial-mesenchymal transition (EMT),...
Source: Biochemistry and Cell Biology - August 15, 2019 Category: Biochemistry Authors: Jiang L, Xie X, Ding F, Mei J, Bi R Tags: Biochem Cell Biol Source Type: research

RP5-1120P11.3 promotes hepatocellular carcinoma development via hsa-miR-196b-5p/WIPF2 axis.
Abstract Hepatocellular carcinoma (HCC) remains a huge threat to human health although the diagnosis and treatment strategies have been improved rapidly in the past few decades. Increasing evidence has illustrated the roles of non-coding RNA and their regulatory network which plays critical roles in pathology of HCC. Here, we identified a novel long non-coding RNA, RP5-1120P11.3 that ectopically expressed in HCC. Further characterization of RP5-1120P11.3 revealed that RP5-1120P11.3 promoted proliferation and invasion of HCC while inhibited apoptosis. Importantly, our data revealed that miR-196b-5p interacted with ...
Source: Biochemistry and Cell Biology - July 12, 2019 Category: Biochemistry Authors: Zhai H, Zhang X, Chen S, Fan M, Ma S, Sun X Tags: Biochem Cell Biol Source Type: research

Atypical chromatin structure of immune-related genes expressed in chicken erythrocytes.
Abstract The major biological role of red blood cells is to carry oxygen to the tissues in the body. However, another role of the erythroid cell is to participate in the immune response. Chicken mature erythrocytes express Toll-like receptors and several cytokines in response to immune stimulation. We previously reported the application of a biochemical fractionation protocol to isolate highly enriched transcribed DNA from chicken polychromatic erythrocytes. In conjunction with next-generation DNA, RNA sequencing, chromatin immunoprecipitation-DNA sequencing and FAIRE sequencing, we identified the active chromosom...
Source: Biochemistry and Cell Biology - July 5, 2019 Category: Biochemistry Authors: Jahan S, Beacon TH, Xu W, Davie JR Tags: Biochem Cell Biol Source Type: research

circ-NOTCH1 acts as a sponge of miR-637 and affects the expression of its target gene Apelin to regulate gastric cancer cell growth.
Abstract Recent studies have indicated that circRNAs have important functions in the occurrence and progression of GC. Studies on circ-NOTCH1, which was shown to be highly expressed in GC, have indicated that miR-637 could bind to circ-NOTCH1 at multiple sites, and a dual-luciferase reporter gene assay further confirmed that miR-637 indeed targeted circ-NOTCH1 and Apelin. Circ-NOTCH1 and Apelin were highly expressed in GC cells and tissues, while the expression of miR-637 was reduced. Circ-NOTCH1 and miR-637 could not regulate each other's expression levels, but circ-NOTCH1 could significantly upregulate the expre...
Source: Biochemistry and Cell Biology - July 5, 2019 Category: Biochemistry Authors: Guan EC, Xu XG, Xue FX Tags: Biochem Cell Biol Source Type: research

MicroRNA-378-3p/5p suppresses migration and invasion of oral squamous carcinoma cells by inhibiting KLK4 expression.
This study aimed to evaluate the role of miR-378 in the migration and invasion of OSCC in vitro and in vivo. According to our results, the migration and invasion abilities were increased in miR-378-overexpressing cells, while decreased in miR-378-3p/5p-silencing cells. In addition, overexpression of miR-378 suppressed the expressions and activities of MMP-9 and MMP-2. Epithelial-mesenchymal transition (EMT) was restrained by overexpression of miR-378 as evidenced by increase in E-cadherin expression and decrease in N-cadherin and uPA expression. However, the miR-378-3p/5p knockdown groups had the opposite results. Moreover...
Source: Biochemistry and Cell Biology - July 2, 2019 Category: Biochemistry Authors: Cui Z, Sun S, Liu Q, Zhou X, Gao S, Peng P, Li Q Tags: Biochem Cell Biol Source Type: research

LncRNA SNHG4 promotes proliferation, migration, invasion and epithelial-mesenchymal transition of lung cancer cells by regulating miR-98-5p.
In conclusion, our findings demonstrate that SNHG4 is required by lung cancer cells to maintain malignant phenotype. SNHG4 probably exerts its pro-survival and pro-metastatic effects by sponging anti-tumor miR-98-5p. PMID: 31220419 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - June 20, 2019 Category: Biochemistry Authors: Tang Y, Wu L, Zhao M, Zhao G, Mao S, Wang L, Liu S, Wang X Tags: Biochem Cell Biol Source Type: research

MiR-19a enhances cell proliferation, migration and invasion through improving lymphangiogenesis via targeting thrombospondin-1 in colorectal cancer.
In conclusion, miR-19a inhibition suppressed the viability, migration and invasion of CRC cells and suppressed migration and tube formation abilities of HLECs, as well as inhibited tumor growth and lymphatic tube formation in vivo via targeting THBS1. PMID: 31199884 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - June 14, 2019 Category: Biochemistry Authors: Yin Q, Wang PP, Peng R, Zhou H Tags: Biochem Cell Biol Source Type: research

Circ_RUSC2 upregulates the expression of miR-661 target gene SYK and regulates the function of vascular smooth muscle cells.
Abstract Nowadays, many studies have found that circRNA is a prospective direction in the field of cardiovascular researches. Detection of circRNA expression in different VSMC phenotypes revealed that circ_RUSC2 was upregulated in proliferative VSMCs. Sequence analysis of circ_RUSC2 showed that there were multiple binding sites of miR-661 on circ_RUSC2, and SYK was an important target gene of miR-661. MiR-661 expression was downregulated in proliferative VSMCs, while SYK expression was upregulated. Circ_RUSC2 and miR-661 did not affect each other expression, but circ_RUSC2 could promote SYK expression and inhibit ...
Source: Biochemistry and Cell Biology - June 14, 2019 Category: Biochemistry Authors: Sun JG, Zhang ZG, Yang SG Tags: Biochem Cell Biol Source Type: research

Non-coding RNAs: what are we missing?
Abstract Over the past two decades, the importance of small non-coding RNAs (sncRNAs) as regulatory molecules has become apparent in all three domains of life. In fact, sncRNAs play an important role in control of gene expression at both the transcriptional and the post-transcriptional level, with crucial roles in fine-tuning cell responses during internal and external stress. Multiple pathways for sncRNA biogenesis and diverse mechanisms of regulation have been reported, and although biogenesis and mechanisms of sncRNAs in prokaryotes and eukaryotes are different, remarkable similarities exist. Here, we briefly r...
Source: Biochemistry and Cell Biology - May 31, 2019 Category: Biochemistry Authors: Carvalho Barbosa C, Calhoun SH, Wieden HJ Tags: Biochem Cell Biol Source Type: research

DNA methylation and regulation of DNA methyltransferases in a freeze tolerant vertebrate.
We examined global DNA methylation and DNA methyltransferases (DNMTs) in the liver and muscle of frozen and glucose-loaded wood frogs. The results showed that 5mC levels increased in the muscle, suggesting elevated DNA methylation during freezing. DNMT activities also decreased in muscle during thawing, glucose loading, and in vitro glucose experiments. Liver DNMT activities were similar to muscle; however, a varied response to DNMT levels and a decrease in 5mC highlight the metabolic role the liver plays during freezing. Glucose was also shown to decrease wood frog DNMT activity levels in vitro, elucidating a potentially ...
Source: Biochemistry and Cell Biology - May 22, 2019 Category: Biochemistry Authors: Zhang J, Hawkins LJ, Storey KB Tags: Biochem Cell Biol Source Type: research

The Impact of Epigenetics on Cardiovascular Disease.
Abstract Mortality and morbidity from the cardiovascular diseases (CVDs) represents a huge burden to society. It is recognized that environmental and individual lifestyle factors play important roles in disease susceptibility but the link between these external risk factors and our genetics was previously unclear. However, the discovery of sequence-independent heritable DNA changes (epigenetics) have helped explain the link between genes and environment. Multiple diverse epigenetic processes, including DNA methylation, histone modification and the expression of non-coding RNA molecules, affect the expression of ge...
Source: Biochemistry and Cell Biology - May 21, 2019 Category: Biochemistry Authors: Prasher D, Greenway SC, Singh RB Tags: Biochem Cell Biol Source Type: research

How to continually make the case for fundamental science - from the perspective of a protein kinase.
Abstract The strength of the scientific process is its immunity from human frailties. The built-in error correction and robustness of principles protect and nurture truth, despite both intended and unintended errors and naivety. What it doesn't secure is understanding of how the scientific sausage is made. Here, a scientific journey revolving around a single protein that spans nearly 35 years is used to illustrate the twists and turns that can accompany any scientific path. Lessons learned from such exploration speak to the need for story-telling in communicating scientific meaning - and the effectiveness of this ...
Source: Biochemistry and Cell Biology - May 21, 2019 Category: Biochemistry Authors: Woodgett JR Tags: Biochem Cell Biol Source Type: research

Label Free Proteomic Analysis Reveals Large Dynamic Changes to the Cellular Proteome Upon Expression of the miRNA-23a-27a-24-2 MicroRNA Cluster.
Abstract In deciphering the regulatory networks of gene expression controlled by the small non-coding RNAs known as microRNAs (miRNAs), a major challenge has been with the identification of the true mRNA targets by these RNAs within the context of the enormous numbers of predicted targets for each of these small RNAs. To facilitate the system wide identification of miRNA targets a variety of system wide methods, such as proteomics, have been implemented. Here we describe the utilization of quantitative label-free proteomics and bioinformatics to identify the most significant changes to the proteome upon expression...
Source: Biochemistry and Cell Biology - May 16, 2019 Category: Biochemistry Authors: Piragasam RS, Hussain SF, Chaulk SG, Siddiqi ZA, Fahlman RP Tags: Biochem Cell Biol Source Type: research

MicroRNA-211-5p promotes the apoptosis and inhibits the migration of osteosarcoma cells by targeting proline-rich protein PRR11.
Abstract Osteosarcoma remains fatal in adolescents and young adults, with a 5-year survival rate of less than 20%. However, the detailed mechanism that regulates osteosarcoma metastasis is poorly understood. We analyzed the expression levels of miR-211-5p in clinical osteosarcoma samples and cell lines, which revealed that miR-211-5p expression was reduced in osteosarcoma. Moreover, induction of miR-211-5p in several osteosarcoma cell lines dramatically inhibited their migration and invasion capacity. Furthermore, miR-211-5p overexpression led to a significant increase of osteosarcoma cell apoptosis. Importantly, ...
Source: Biochemistry and Cell Biology - May 10, 2019 Category: Biochemistry Authors: Song D, Yang K, Wang W, Tian R, Wang H, Wang K Tags: Biochem Cell Biol Source Type: research

Mitochondrial dysfunction regulates the JAK/STAT pathway by LKB1-mediated AMPK activation in an ER stress-independent manner.
Abstract Mitochondria affect cellular functions alone or in cooperation with other cellular organelles. Recent research has demonstrated the close relationship of mitochondria with the endoplasmic reticulum (ER), both at the physical and the functional level. In an effort to define the combined effect mitochondrial dysfunction (MD) and ER stress in the proinflammatory activities of macrophages, the human macrophage-like monocytic leukemia cell line THP-1 was treated with mitochondrial electron transport chain (ETC) blockers, and changes in the cellular responses upon stimulation by interferon (IFN)-γ were an...
Source: Biochemistry and Cell Biology - May 9, 2019 Category: Biochemistry Authors: Kim DY, Lim SG, Suk K, Lee WH Tags: Biochem Cell Biol Source Type: research

Sirt6 stabilizes atherosclerosis plaques by promoting macrophage autophagy and reducing contact with endothelial cells.
In conclusion, our study indicates a new mechanism-based strategy to therapeutically stimulate atherosclerosis plaque stability. PMID: 31063699 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - May 7, 2019 Category: Biochemistry Authors: Wang T, Sun C, Hu L, Gao E, Li C, Wang H, Sun D Tags: Biochem Cell Biol Source Type: research

Particulate matter with a diameter of ≤2.5 μm induces and enhances bleomycin-induced pulmonary fibrosis by stimulating endoplasmic reticulum stress in rat.
This study was designed to investigate the effect of particulate matter with a diameter of ≤2.5 μm (PM2.5) on bleomycin (BLM) induced pulmonary fibrosis. Thirty-two Sprague Dawley rats were assigned into four groups (intratracheal instillation of 500 μL of PBS (control), 2 mg/kg PM2.5, 3.5 mg/kg BLM A5, and BLM plus 2.0 mg/kg PM2.5) and were fed for 14 days. All rats were sacrificed after the study. Lung tissues and bronchoalveolar lavage fluid were prepared for histological and biological analysis. We found that PM2.5 caused dose-trend pulmonary alveolitis and fibrosis. Histological scores, expression of α-...
Source: Biochemistry and Cell Biology - May 6, 2019 Category: Biochemistry Authors: Xu P, Yao Y, Zhou J Tags: Biochem Cell Biol Source Type: research

Role of syndecan-1 and exogenous heparin in hepatoma sphere formation.
In conclusion, we found that SDC1 affected CSC marker CD13 and CD44 expression. SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development. Application of heparin mimics in reduction of hepatoma stem cells might be potential. PMID: 31042409 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - May 1, 2019 Category: Biochemistry Authors: Lin SC, Wu CP, Tseng T, Jhang Y, Lee S Tags: Biochem Cell Biol Source Type: research

miR-628-5p promotes the growth and migration of osteosarcoma by targeting IFI44L.
This study investigated the role of miR-628-5p and interferon-induced protein 44-like (IFI44L) in osteosarcoma (OS) and determined whether miR-628-5p modulated OS growth by regulating IFI44L. Based on the data downloaded from Gene Expression Omnibus (GEO) database, we revealed that the expression of IFI44L was down-regulated in OS and low expression of IFI44L was correlated with better prognosis of patients with OS. Biological prediction of its upstream regulatory miRNAs on the miRWalk website found that miR-628-5p is a possible upstream regulatory miRNA of IFI44L. Luciferase activity assay demonstrated that miR-628-5p cou...
Source: Biochemistry and Cell Biology - April 24, 2019 Category: Biochemistry Authors: Wang JY, Lu SB, Wang JQ Tags: Biochem Cell Biol Source Type: research

Propofol promotes apoptosis of colorectal cancer cells via alleviating the suppression of lncRNA HOXA11-AS on miRNA let-7i.
Abstract Recently, surgical resection is still the mainstay for colorectal cancer (CRC) treatment. Propofol (2,6-diisopropylphenol), one of the most commonly used intravenous anaesthetic agents, has been reported to be involved in modulating the malignancy of a variety of human cancers. However, the underlying mechanisms remain poorly understood. In the present study, we found that propofol could promote cell apoptosis and inhibit cell proliferation using Cell Counting Kit-8 (CCK-8), flow cytometry and caspase 3 cleavage assays, which causing the down-regulation of HOXA11-AS and up-regulation of let-7i in both Col...
Source: Biochemistry and Cell Biology - April 23, 2019 Category: Biochemistry Authors: Ren YL, Zhang W Tags: Biochem Cell Biol Source Type: research

Long non-coding RNA XIST regulates the EGF receptor to promote TGF- β1-induced epithelial-mesenchymal transition in pancreatic cancer.
CONCLUSIONS: XIST promotes TGF-β1-induced EMT by regulating miR-34a/YAP/EGFR axis in PC. PMID: 31013436 [PubMed - as supplied by publisher] (Source: Biochemistry and Cell Biology)
Source: Biochemistry and Cell Biology - April 23, 2019 Category: Biochemistry Authors: Zou L, Chen FR, Xia RP, Wang HW, Xie ZR, Xu Y, Yu JH, Wang KH Tags: Biochem Cell Biol Source Type: research