Metalloproteinase inhibitor GM6001 delays regeneration in holothurians.
Abstract The effect of the GM6001 metalloproteinase inhibitor on the regeneration of ambulacral structures in Eupentacta fraudatrix has been investigated. Inhibition of proteinase activity exerts a marked effect on regeneration, being dependent on the time when GM6001 is injected. When administration of the inhibitor begins on day 3 post-injury, regeneration is completely abolished, and the animals die. This means that early activation of proteinases is crucial for triggering the regenerative process in holothurians. When GM6001 in first injected on day 7 post-injury, the regeneration rate decreases. However, this...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Dolmatov IY, Shulga AP, Ginanova TT, Eliseikina MG, Lamash NE Tags: Tissue Cell Source Type: research
Morphology and cytochemical patterns of peripheral blood cells in domestic pigeon (Columba livia).
In this study, a comprehensive investigation on the morphology and cytochemical patterns of peripheral blood cells in domestic pigeons were conducted by using wright's and various cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(ALP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Besides erythrocytes and thrombocytes, five types of leukocytes were identified: heterophils, eosinophils, basophils, lymphocytes and monocytes. Lymphocytes were the most abundant leuko...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Chen X, Wang J, Wei Q, Hanif M, Li E, Zhang S Tags: Tissue Cell Source Type: research
Gemcitabine, vinorelbine and cyclooxygenase inhibitors in the treatment of glioblastoma. Ultrastructural analyses in C6 glioma in vitro.
CONCLUSIONS: Detailed ultrastructural analysis of the effects of chemotherapeutic drugs may provide a broader insight about their actions and pave to develop better strategies in treatment of glioblastoma. PMID: 31383285 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Elmaci İ, Bilir A, Ozpinar A, Altinoz MA Tags: Tissue Cell Source Type: research
Expression of anti-aging type-XVII collagen (COL17A1/BP180) in hair follicle-associated pluripotent (HAP) stem cells during differentiation.
Abstract Hair-follicle-associated pluripotent (HAP) stem cells reside in the upper part of the bulge area of the the hair follicle. HAP stem cells are nestin-positive and keratin 15-negative and have the capacity to differentiate into various types of cells in vitro. HAP stem cells are also involved in nerve and spinal cord regeneration in mouse models. Recently, it was shown that the DNA-damage response in non-HAP hair follicle stem cells induces proteolysis of type-XVII collagen (COL17A1/BP180), which is involved in hair-follicle stem-cell maintenance. COL17A1 proteolysis stimulated hair-follicle stem-cell aging...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Shirai K, Obara K, Tohgi N, Yamazaki A, Aki R, Hamada Y, Arakawa N, Singh SR, Hoffman RM, Amoh Y Tags: Tissue Cell Source Type: research
A 3D tumor spheroid model for the T98G Glioblastoma cell line phenotypic characterization.
Abstract Major Glioblastoma's hallmarks include proliferation, invasion and heterogeneity. Biological 3D tumor spheroid models can serve as intermediate systems between traditional 2D cell culture and complex in vivo models. Tumor spheroids have been shown to more accurately reproduce the spatial organization and microenvironmental factors of in vivo micro-tumors, such as relevant gradients of nutrients and other molecular agents, while they maintain cell-to-cell and cell-to-matrix interactions. In vitro 3D assays are useful to monitor these properties. Here, we test the suitability of the well-known T98 G Gliob...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Oraiopoulou ME, Tampakaki M, Tzamali E, Tamiolakis T, Makatounakis V, Vakis AF, Zacharakis G, Sakkalis V, Papamatheakis J Tags: Tissue Cell Source Type: research
The importance of insect sperm: Sperm ultrastructure of Hermetia illucens (black soldier fly).
This study confirms sperm structure diversity between brachyceran subspecies and support its relationship with nematoceran subspecies. PMID: 31383288 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Kotzé RCM, Muller N, du Plessis L, van der Horst G Tags: Tissue Cell Source Type: research
Notch signaling regulates osteosarcoma proliferation and migration through Erk phosphorylation.
Abstract We used a murine spontaneous osteosarcoma cell line with high metastatic potential, the K7M2 cell line to study the role of Notch signaling in the biological manifestations of osteosarcoma, to understand its underlying mechanism in the regulation of cell proliferation and migration, and to improve patient prognosis in cases of osteosarcoma through the discovery of novel therapeutic targets, First, Notch expression in K7M2 was determined by immunostaining, and the γ-secretase inhibitor N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) was used to inhibit proteolytic cleavag...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Qin J, Wang R, Zhao C, Wen J, Dong H, Wang S, Li Y, Zhao Y, Li J, Yang Y, He X, Wang D Tags: Tissue Cell Source Type: research
Azoramide ameliorates fructose-induced nonalcoholic fatty liver disease in mice.
Abstract Nonalcoholic fatty liver disease (NAFLD) is a frequent health problem. The insulin resistance and endoplasmic reticulum (ER) stress have been suggested to play important roles in the development and progression of NAFLD. However these processes and correlations have not fully been understood yet. Azoramide, an antidiabetic drug, has the potential for reducing insulin resistance and ER stress in obese mice. To date, there is no study on the effects of azoramide in NAFLD. The aim of this study was to investigate the potential role of azoramide on insulin resistance and ER stress in NAFLD induced mice. Forty...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Bagci R, Sahinturk V, Sahin E Tags: Tissue Cell Source Type: research
Recent trends in peripheral nervous regeneration using 3D biomaterials.
Abstract Mesenchymal stem cells (MSCs) owing their multipotency are known as progenitors for the regeneration of adult tissues including that of neuronal tissue. The repair and/or regeneration of traumatic nerves is still a challenging task for neurosurgeons. It is also a well-established fact that the microenvironment plays a primary role in determining the fate of stem cells to a specific lineage. In recent years, with the advent of nanotechnology and its positive influence on designing and fabrication of various 3D biomaterials have progressed to a greater extent. The production of 3D biomaterials such as nanof...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Ashraf R, Sofi HS, Beigh MA, Sheikh FA Tags: Tissue Cell Source Type: research
Segmentation and analysis of surface characteristics of oral tissues obtained by scanning electron microscopy to differentiate normal and oral precancerous condition.
Abstract Abnormal epithelial stratification is a sign of oral dysplasia and hence evaluation of surface characteristics of oral epithelial region can help in detection of cancerous progression. Surface characteristics can be better visualised by Scanning Electron Microscopy (SEM) in comparison to light microscopy. In our study we have developed automated image processing algorithms i.e. Gaussian with median filtering and Gradient filtering, using MATLAB 2016b, to segment the surface characteristics i.e. the ridges and pits in the SEM images of oral tissue of normal (13 samples) and Oral Submucous Fibrosis (OSF) (3...
Source: Tissue and Cell - August 1, 2019 Category: Cytology Authors: Nag R, Pal M, Paul RR, Chatterjee J, Kumar Das R Tags: Tissue Cell Source Type: research
Midazolam prevents sevoflurane-induced death in hippocampal neurons.
In this study, we identified the neuroprotective role of midazolam in hippocampal neurons. Midazolam treatment could alleviate the neuronal death and promote the neuronal maturation in hippocampal neurons in vitro. In vivo studies demonstrated that midazolam injection could improve behavioral deficit in sevoflurane-exposed animals. The anti-apoptotic function of midazolam in sevoflurane-exposed neurons was mediated by ERK signaling. Collectively, we elucidated a new role of midazolam in preventing hippocampal neuronal death from sevoflurane exposure, potentially providing a new strategy to resist the neurotoxicity in the c...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Yu D, Zhu Y, Cui C, Long R, Ma J Tags: Tissue Cell Source Type: research
Pazopanib regresses a doxorubicin-resistant synovial sarcoma in a patient-derived orthotopic xenograft mouse model.
Abstract Synovial sarcoma (SS) is an aggressive subgroup of soft tissue sarcoma (STS) with high grade and high risk of metastasis. However, there are no systemic therapies available that target SS. Therefore, transformative therapy is needed for SS. To establish a patient-derived orthotopic xenograft (PDOX) model, a patient tumor with high grade SS from a lower extremity was grown orthotopically in the right biceps femoris muscle of mice. To test the efficacy of drugs, the PDOX models were randomized into five groups: Group 1 (G1), control-without treatment; Group 2 (G2), doxorubicin (DOX); Group 3 (G3), temozolom...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Higuchi T, Singh SR, Tsuchiya H, Hoffman RM Tags: Tissue Cell Source Type: research
Expression of apoptosis inhibitor of macrophages in tissue macrophages, leukocytes and vascular endothelial cells of dogs.
In this study, to elucidate the physiological expression pattern of AIM in dogs, systemic expression and distribution of AIM of dogs were investigated. Mature healthy Beagles were used. Various tissues, peripheral blood cells, and bone marrow cells of normal dogs were collected for in situ hybridization, real-time RT-PCR, and immunohistochemistry. AIM mRNA and protein were expressed in tissue macrophages of the spleen, liver, lungs, and lymph nodes, but not in the microglia of the cerebrum. Proximal tubules in the kidney also expressed AIM protein. Monocytes and B lymphocytes in circulating blood and a part of microvascula...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Uchida M, Uchida K, Maeda S, Yonezawa T Tags: Tissue Cell Source Type: research
Comparison between two programs for image analysis, machine learning and subsequent classification.
Abstract In the early 1950s, flow cytometry was developed as the first method for automated quantitative cellular analysis. In the early 1990s, the first equipment for image cytometry (laser scanning cytometry, LSC) became commercially available. As flow cytometry was considered the gold standard, various studies found that the results of flow cytometry and LSC generated comparable results. One of the first programs for image analysis that included morphological parameters was ImageJ, published in 1997. One of the newer programs for image analysis that is not limited to fluorescence images is the free software Cel...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Ribeiro GP, Endringer DC, De Andrade TU, Lenz D Tags: Tissue Cell Source Type: research
Argon plasma-treated fluorinated ethylene propylene: Growth of primary dermal fibroblasts and mesenchymal stem cells.
ová NS, Švorčík V, Ruml T Abstract Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article, we show that FEP modified with Ar plasma with the power of 3 and 8 W for 40 and 240 s served as a suitable material for cultivation of primary human dermal fibroblasts (HDF), which showed significantly improved proliferation and spreadin...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Peterková L, Rimpelová S, Slepička P, Křížová I, Kasálková NS, Švorčík V, Ruml T Tags: Tissue Cell Source Type: research
Soy hydrolysate mimic autocrine growth factors effect of conditioned media to promote single CHO-K1 cell proliferation.
Abstract The increasingly competitive biopharmaceutical industry requires companies to focus on rapid and low-cost cell line development. Single-cell cloning (SCC) is a critical and high-value process for cell line development, and typically problematic because single cell proliferates slowly when cultivated at low cell densities. Conditioned media (CM) provide autocrine growth factors to facilitate single cell proliferation, thus improve SCC efficiency. However, conditioned media (CM) are not a feasible solution for industrial cell line development due to variation and cross-contamination concerns. Here, we have ...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Xu W, Yu X, Zhang J, Bhushan S, Prasad S, Prasad KN, Wu F, Yuan J, Poon HF Tags: Tissue Cell Source Type: research
Temozolomide targets and arrests a doxorubicin-resistant follicular dendritic-cell sarcoma patient-derived orthotopic xenograft mouse model.
Abstract Follicular dendritic cell sarcoma (FDCS) is a very rare and highly recalcitrant disease. A patient's doxorubicin-resistant FDCS was previously established orthotopically on the right high thigh into the biceps femoris of mice to establish a patient-derived orthotopic xenograft (PDOX) model. The aim of the present manuscript was to identify an effective drug for this recalcitrant tumor. Here, we evaluated the efficacy of temozolomide (TMZ), trabectedin (TRAB) and pazopanib (PAZ) on the FDCS PDOX model. PDOX mouse models were randomized into five groups of eight to nine mice, respectively. Group 1, untreate...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Oshiro H, Tome Y, Kiyuna T, Miyake K, Kawaguchi K, Higuchi T, Miyake M, Zang Z, Razmjooei S, Barangi M, Wangsiricharoen S, Nelson SD, Li Y, Bouvet M, Singh SR, Kanaya F, Hoffman RM Tags: Tissue Cell Source Type: research
Seminiferous tubule molecular imaging for evaluation of male fertility: Seeing is believing.
Abstract The proper assessment of male fertility is essential for diagnosing and treating male infertility. Currently, spermiogram and Johnsen testicular biopsy score counts are used to assess male fertility. However, spermiogram is not a suitable option for non-obstructive azoospermia patients, and Johnsen testicular biopsy scores only represent localized and not the overall spermatogenesis. Whole-mount staining was a novel method for evaluating protein expression in the tissue. Thus, we explored its application in human seminiferous tubules. Testicular biopsies from 57 azoospermia patients were categorized as ob...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Yao C, Zhao L, Tian R, Li P, Zhu Z, Xue Y, Chen H, Gong Y, Liu N, Yang C, He Z, Li Z Tags: Tissue Cell Source Type: research
PLLA scaffolds with controlled architecture as potential microenvironment for in vitro tumor model.
bba V Abstract The "microenvironment" where a tumor develops plays a fundamental role in determining its progression, the onset of metastasis and, eventually, its resistance to therapies. Tumor cells can be considered more or less invasive depending both on the nature of the cells and on the site where they are located. Commonly adopted laboratory culture protocols for the investigation of tumor cells take usually place on standard two-dimensional supports. However, such cultures do not allow for reproduction of the biophysical properties of the tumor's microenvironment, thus causing the cells to lose mo...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Lombardo ME, Carfì Pavia F, Vitrano I, Ghersi G, Brucato V, Rosei F, La Carrubba V Tags: Tissue Cell Source Type: research
Distribution of postganglionic neurons which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human middle cervical ganglion.
In this study, immunohistochemistry for catecholamine-synthesizing enzymes and neuropeptides was performed on the MCG in human cadavers. In 4 samples of human cadavers, MCG swellings contained numerous postganglionic neurons. In another sample, a distinct swelling of the MCG could not be detected. However, neuronal cell bodies were present within the sympathetic nerve trunk between the superior cervical and stellate ganglia. The cell size analysis demonstrated that cell bodies of postganglionic neurons measured 94.1-1774.1 μm2 (mean ± S.D. = 578.1 ± 127.7&th...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Kokubun S, Sato T, Yajima T, Ichikawa H Tags: Tissue Cell Source Type: research
In vitro heterogeneity of porcine adipose tissue-derived stem cells.
is I Abstract Tissue-specific adult stem cells (ASC) are heterogeneous and characterized by a mix of progenitor cells that produce cells at various stages of differentiation, and ultimately different terminally differentiated cells. Understanding the heterogeneity of ASCs may lead to the development of improved protocols of cell isolation and optimized cell therapy clinical protocols. Using a combination of enzymatic and explant culture protocols, we obtained pADSC population, which is composed by two distinct morphologies: fibroblast-like cells (FLCs) and endothelial-like cells (ELCs). Both cell sub-types efficie...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Santos Rizzo Zuttion MS, Dias Câmara DA, Dariolli R, Takimura C, Wenceslau C, Kerkis I Tags: Tissue Cell Source Type: research
Effect of CREB1 promoter non-CpG island methylation on its differential expression profile on sheep ovaries associated with prolificacy.
This study aimed to investigate the effect of different methylated regions of cyclic-AMP response element binding protein 1 (CREB1) by comparing the high prolificacy (HP) group and low prolificacy (LP) group, which was detected in our previous study. The expression level of CREB1 mRNA in the ovaries of the HP group was higher than in the LP group (P
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Zhao J, Li FZ, Wu J, Yang H, Zheng J, Pang J, Meng FX, Wang F, Zhang YL Tags: Tissue Cell Source Type: research
Characterization and expression profiles of cyp19a1a in the schizothoracine fish Schizothorax prenanti.
Abstract Aromatase plays a central role in ovarian differentiation and development in teleosts. In the present study, we identified a cyp19a1a homologue from the ovary of Schizothorax prenanti and analysed its expression at both the mRNA and protein levels. Cyp19a1a of S. prenanti showed high homology with that of other teleosts, especially S. kozlovi. The ovary and testis were the main sites of cyp19a1a expression in S. prenanti, and cyp19a1a transcript levels peaked in the mid-vitellogenic (MVG)-stage ovary and the mid-spermatogenic (MS)-stage testis. Signals of Cyp19a1a immunopositivity were detected in the spe...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Yan T, Cai Y, He J, Zhang Q, Wang X, Zhang S, He L, He Z Tags: Tissue Cell Source Type: research
A comparative study of deep learning architectures on melanoma detection.
Abstract Melanoma is the most aggressive type of skin cancer, which significantly reduces the life expectancy. Early detection of melanoma can reduce the morbidity and mortality associated with skin cancer. Dermoscopic images acquired by dermoscopic instruments are used in computational analysis for skin cancer detection. However, some image quality limitations such as noises, shadows, artefacts exist that could compromise the robustness of the skin image analysis. Hence, developing an automatic intelligent system for skin cancer diagnosis with accurate detection rate is crucial. In this paper, we evaluate the per...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Hosseinzadeh Kassani S, Hosseinzadeh Kassani P Tags: Tissue Cell Source Type: research
Accessory structures that originate from photoreceptor bundles in the retina of the teleost Notopterus notopterus (Pallas).
Abstract The teleost species Notopterus notopterus (Pallas) possess bundled photoreceptors in their retina. It was found that the margin of the inner portion (the vitreal half) of photoreceptor bundles emits thin processes. Each process terminates on the contralateral photoreceptor bundle, or the processes from adjacent photoreceptor bundle may fuse. The site of the inner half of the photoreceptor bundles from where they arise shows minimal support of the photoreceptor bundles by retinal pigment epithelium, and so it is likely that those accessory structures may aid to hold the photoreceptor bundles in correct ori...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Nag TC Tags: Tissue Cell Source Type: research
A biomimetic cartilage gradient hybrid scaffold for functional tissue engineering of cartilage.
In this study, chitosan/Sodium β-glycerophosphate/Gelatin (Cs/GP/Gel) biomimetic gradient scaffolds were prepared by regulating the mass ratio of single layer raw materials. The same ratio of Cs/GP/Gel hybrid scaffold material was used as the control. Physical properties such as water absorption, porosity and the degradation rate of the material were compared to optimize the proportion of scaffold materials. P3 Bone Mesenchymal Stem Cells (BMSCs) were inoculated on the gradient and the control scaffolds to investigate its biocompatibility. Scanning electron microscopy (SEM) results show that 3:1:2, 6:1:3.5, 9:1:5, 12:...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Hu X, Li W, Li L, Lu Y, Wang Y, Parungao R, Zheng S, Liu T, Nie Y, Wang H, Song K Tags: Tissue Cell Source Type: research
An improved method of culturing somatotropic cells from rat adenohypophysis.
This study aimed to propose a simple and practical method for culturing primary rat somatotropic cells in vitro free of pericytes contamination. Rat adenohypophyses were randomly divided into two groups. An improved method was used in group A (digesting adenohypophysis with 0.25% trypsin-EDTA, followed by removing pericytes by double filtration and using serum-free medium for culturing somatotropic cells). The traditional method was used in group B (digesting adenohypophysis with 0.35% collagenase, using serum medium for culturing somatotropic cells, and removing pericytes by changing the culture dish). The numbers and via...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Mao J, Bao Y, Mei F, Liao X, Liu F, Zhou L, Qi S, Qiu B Tags: Tissue Cell Source Type: research
Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation.
AC, Oliveira Favaron P Abstract The amniotic membrane can be considered as one of the sources of isolation of these cells, since it is found in the fetal maternal interface and has low immunogenicity. Mesenchymal stromal/stem cells (MSCs) have not been identified in canine amniotic membrane (AMC). Therefore, our objective was to isolate, culture, characterize and differentiate cells derived from canine amniotic membrane (AMC) and to verify its immunological and tumorigenic potential. For this, 12 dogs fetuses of each gestational age 32, 43 and 55 days were used, and the isolation and culture of the AMC were perfo...
Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Borghesi J, Ferreira Lima M, Mario LC, de Almeida da Anunciação AR, Silveira Rabelo AC, Giancoli Kato Cano da Silva M, Assunpção Fernandes F, Miglino MA, Oliveira Carreira AC, Oliveira Favaron P Tags: Tissue Cell Source Type: research
Ultrastructural organisation of the spermatozoon of Allopodocotyle tunisiensis Derbel and Neifar, 2009 (Digenea, Opecoelidae), an intestinal parasite of Solea aegyptiaca Chabanaud, 1927 (Teleostei, Soleidae).
Abstract The ultrastructure of the spermatozoon of Allopodocotyle tunisiensis (Digenea, Opecoelidae), an intestinal parasite of Solea aegyptiaca (Teleostei, Soleidae), is described by transmission electron microscopy (TEM). The mature spermatozoon is a filiform cell that exhibits two axonemes of different length with the 9+'1' pattern of trepaxonematan Platyhelminthes. In the anterior spermatozoon extremity, cortical microtubules are absent. They appear after the disappearance of an anterior electron-dense material, being initially in a continuous and submembranous layer. They surround only partially the sperm cel...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Kacem H, Diagne PM, Miquel J Tags: Tissue Cell Source Type: research
Immunophenotypic characterization, multi-lineage differentiation and aging of zebrafish heart and liver tissue-derived mesenchymal stem cells as a novel approach in stem cell-based therapy.
In this study, mesenchymal stem cells (MSCs) were isolated from heart and liver tissue of Zebrafish (Danio rerio). The flow-cytometry as well as RT-PCR were used to analyze the expression of a panel of cell surface markers CD44, CD90, CD31 and CD34. In the following, alizarin red, oil red-O and toluidine blue staining were carried out to evaluate the multi-lineage differentiation of zebrafish heart and liver tissue-derived MSCs. Subsequently, the gene and protein expression of Oct4, Sox2 and Nanog as pluri-potent markers were analyzed by RT-PCR and western blotting, respectively. In addition, MTT assay was used for cell pr...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Fathi E, Farahzadi R, Sheikhzadeh N Tags: Tissue Cell Source Type: research
Expression of miR-210 mediated by adeno-associated virus performed neuroprotective effects on a rat model of acute spinal cord injury.
Abstract Acute spinal cord injuries (ASCI) are common neural disorders in traumatology medicine. MicroRNA-210 (miR-210) plays a crucial role in cell survival, endothelial cell migration and cell regeneration. This paper is aim to validate the pathophysiological function of miR-210 on ASCI. We built a rat model of ASCI and utilized an adeno-associated virus (rAAV)-expressing miR-210 for stable over-expression of miR-210. We tested in vivo miR-210 gain of function on ASCI by microinjected rAAV-miR-210 into the rat spinal cord. We further screened the targeting genes of miR-210 by PCR array and detected relate...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Li R, Bao L, Hu W, Liang H, Dang X Tags: Tissue Cell Source Type: research
Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius).
wak B Abstract Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had ...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Dang M, Nowell C, Nguyen T, Bach L, Sonne C, Nørregaard R, Stride M, Nowak B Tags: Tissue Cell Source Type: research
Comparison of human articular chondrocyte and chondroprogenitor cocultures and monocultures: To assess chondrogenic potential and markers of hypertrophy.
CONCLUSION: In conclusion, this study suggests the need for improved sorting techniques based on a characteristic differentiating biomarker for selection of cells which are true representatives of CPs possessing properties of enhanced chondrogenesis and reduced hypertrophy. PMID: 30947962 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Vinod E, Kachroo U, Ozbey O, Sathishkumar S, Boopalan PRJVC Tags: Tissue Cell Source Type: research
In vivo articular cartilage regeneration through infrapatellar adipose tissue derived stem cell in nanofiber polycaprolactone scaffold.
In this study, we report the development of a nanofiber polycaprolactone scaffold that can act as a stem cell carrier to induce chondrogenesis and promote cartilage repair in vivo. Infrapatellar fat pads were obtained from sheep knee and the stem cells were isolated and characterized by flow cytometry. Defects were created in sheep knee, two defects received adipose tissue derived stem cells (ASCs)-polycaprolactone construct, second group received polycaprolactone (PCL), the third group was chosen as the ASCs group and the fourth group was control group. Morphological evaluation showed that defects treated with ASCs-scaffo...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Vahedi P, Jarolmasjed S, Shafaei H, Roshangar L, Soleimani Rad J, Ahmadian E Tags: Tissue Cell Source Type: research
Protective effects of exercise on heart and aorta in high-fat diet-induced obese rats.
Abstract We investigated the protective effects of swimming exercise on high-fat diet-induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide (NO) system. Sprague Dawley rats were fed either standard chow (STD, 6% fat) or high-fat diet (HFD; 45% fat) for 18 weeks, with half of the animals trained by daily swimming sessions (EXC; 1 h per day for 5 days/week) for the last 6 weeks of the experimental period and half kept sedentary (SED). Heart and aorta tissues were prepared for routine light and electron microscopy evaluation. Endothelial NOS (eNOS) and inducible NOS (iN...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Acikel Elmas M, Cakıcı SE, Dur IR, Kozluca I, Arınc M, Binbuga B, Bingol Ozakpınar O, Kolgazi M, Sener G, Ercan F Tags: Tissue Cell Source Type: research
Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus).
This study is the first to provide comprehensive details on the ultrastructure of spermatozoa in E. maclovinus. PMID: 30947965 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Ulloa-Rodríguez P, Contreras P, Dumorné K, Figueroa E, Risopatrón J, Valdebenito I, Farías JG Tags: Tissue Cell Source Type: research
Effect of royal jelly on testicular antioxidant enzymes activity, MDA level and spermatogenesis in rat experimental Varicocele model.
Abstract Varicocele is one of the most prevalent causes of infertility. It causes induction of oxidative stress, increases lipid peroxidation in the testis and disrupts spermatogenesis cycle. The aim of the study was to investigate the possible protective effects of royal jelly against varicocele induced oxidative stress, biochemical and histological alterations in the experimental varicocele model in rat. Twenty-one adult Wistar rats were divided into three groups. The control group (I), Varicocele and administration of normal saline (II), varicocele and treatment with RJ (III). At the end of the experiment, all ...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Asadi N, Kheradmand A, Gholami M, Saidi SH, Mirhadi SA Tags: Tissue Cell Source Type: research
The histomorphometric parameters of endocrine pancreas after bariatric surgery in healthy animal models.
CONCLUSION: Our purpose was to show a comprehensive interpretation to the consequences that bariatric surgeries had on the pancreatic islets cellularity. Moreover, we included the main tests to report the cellularity in histological samples. PMID: 30947967 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Camacho-Ramírez A, Almorza-Gomar D, Díaz-Gómez A, Mateo-Gavira I, Macias-Rodriguez M, Pérez-Arana GM, Prada-Oliveira JA Tags: Tissue Cell Source Type: research
Comparative assessment of CNN architectures for classification of breast FNAC images.
Abstract Fine needle aspiration cytology (FNAC) entails using a narrow gauge (25-22 G) needle to collect a sample of a lesion for microscopic examination. It allows a minimally invasive, rapid diagnosis of tissue but does not preserve its histological architecture. FNAC is commonly used for diagnosis of breast cancer, with traditional practice being based on the subjective visual assessment of the breast cytopathology cell samples under a microscope to evaluate the state of various cytological features. Therefore, there are many challenges in maintaining consistency and reproducibility of findings. However, the ...
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Saikia AR, Bora K, Mahanta LB, Das AK Tags: Tissue Cell Source Type: research
The prognostic value of sex hormone receptors expression in laryngeal carcinoma.
In conclusion, ER-β and PR may be considered as markers for poor biological behavior of laryngeal carcinoma. PMID: 30947969 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - April 1, 2019 Category: Cytology Authors: Atef A, El-Rashidy MA, Elzayat S, Kabel AM Tags: Tissue Cell Source Type: research
Merkel cells immunohistochemical study in striped dolphin (Stenella coeruleoalba) skin.
gio C Abstract Cetacean mechanical senses, such as hearing, echolocation, active touch and the perception of water movements, are essential for their survival. Dolphins skin possesses dense packing of dermal papillae associated with the cutaneous ridges that suggests a sensory function, furthermore they are well innervated and very sensitive to touch. This is mediated by mechanoreceptors, abundant in the region of the head and in the dorsal part of the body. Most odontocetes possess vibrissae (i.e., sensory hair) that have been well described in literature and present a microanatomy similar to that of terrestrial ...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Lauriano ER, Pergolizzi S, Aragona M, Spanò N, Guerrera MC, Capillo G, Faggio C Tags: Tissue Cell Source Type: research
Allogeneic platelet rich plasma serves as a scaffold for articular cartilage derived chondroprogenitors.
Abstract Limited self-restorative ability of the cartilage has necessitated the use of cell and tissue engineering based therapies. Recent advances in the isolation, expansion and characterization of articular cartilage derived chondroprogenitors(CPs) has gained popularity in its role for cartilage repair. Platelet rich plasma (PRP) is a reliable biological scaffold for in-vitro and in-vivo studies with reported therapeutic applications in cartilage and bone pathologies. The aim of this study was to evaluate whether human allogeneic PRP could serve as a biological scaffold for chondroprogenitors (CPs) in cartilage...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Vinod E, Vinod Francis D, Manickam Amirtham S, Sathishkumar S, Boopalan PRJVC Tags: Tissue Cell Source Type: research
Dimethyl fumarate up-regulates expression of major neurotrophic factors in the epidermal neural crest stem cells.
Abstract There is an agreement that combining treatments can lead to substantial improvement, therefore the present study assessed the effects of different concentrations of dimethyl fumarate (DMF) on viability of epidermal neural crest stem cells (EPI-NCSCs). In addition, this investigation was designed to evaluate the effects of DMF on relative expression of major trophic factors mainly the ones with neurotrophic effects, expressed in EPI-NCSCs in order to enhance their therapeutic potential. To determine the appropriate concentration of DMF for EPI-NCSCs treatment, the MTT assay was employed and based on the ob...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Salehi MS, Borhani-Haghighi A, Pandamooz S, Safari A, Dargahi L, Dianatpour M, Tanideh N Tags: Tissue Cell Source Type: research
Anti-fibrotic effect of black tea (Camellia sinensis) extract in experimental pulmonary fibrosis.
This study aims to investigate the anti-fibrotic effect of BTE against experimental pulmonary fibrosis. Four groups of animals were selected for this study. Group 1: control group mice. Group 2: mice exposed to bleomycin for 21 days, Group 3 and Group 4: bleomycin exposed mice treated with 25 mg BTE/kg b.w./day, p.o and 50 mg BTE/kg b.w./day, p.o. respectively for 21 days. Bleomycin exposed mice showed increased collagen deposition and wet/dry weight ratio, which were attenuated upon 50 mg BTE/kg b.w. treatment. The increased level of histopathological parameters in bleomycin-induced mice was significa...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Chakraborty K, Dey A, Bhattacharyya A, Dasgupta SC Tags: Tissue Cell Source Type: research
Adipose derived mesenchymal stem cells improve the structure and function of autografted mice ovaries through reducing oxidative stress and inflammation: A stereological and biochemical analysis.
Abstract The aim was to investigate the effect of injecting adipose derived mesenchymal stem cells (ADSCs) on reducing oxidative stress, inflammation and improving the structure and function of the autografted ovaries through stereological and biochemical evaluations. Mice (4-5 weeks old) were divided into three groups: control, autograft, and autograft + ADSCs (six mice per group). 7 days after ovary autografting and ADSCs injection, serum concentrations of IL-6, TNF-α and IL-10, malondialdehyde and superoxide dismutase activity were measured. On day 28, ovary histology and CD31 expression was...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Shojafar E, Soleimani Mehranjani M, Shariatzadeh SMA Tags: Tissue Cell Source Type: research
Reactivity of microglia and astrocytes after an excitotoxic injury induced by kainic acid in the rat spinal cord.
Abstract After injury of the nervous system glial cells react according to the stimuli by modifying their morphology and function. Glia activation was reported in different kainic acid (KA)-induced neurodegeneration models. Here, we describe glial morphometric changes occurring in an excitotoxic KA-induced cervical spinal cord injury model. Concomitant degenerative and apoptotic processes are also reported. Male rats injected at the spinal cord C5 segment either with KA or saline were euthanized at post-injection (PI) days 1, 2, 3 or 7. Anti-IBA-1 and anti-GFAP antibodies were used to identify microglia and activa...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Zanuzzi CN, Nishida F, Sisti MS, Barbeito CG, Portiansky EL Tags: Tissue Cell Source Type: research
The effect of docosahexaenoic acid on apelin distribution of nervous system in the experimental mouse model of Parkinson's disease.
In conclusion; DHA has been implicated in the expression of the apelin receptor and has reduced the expression of APJ receptor. PMID: 30736903 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Acar N, Parlak H, Ozkan A, Soylu H, Avcı S, Ustunel I, Izgut-Uysal VN, Agar A Tags: Tissue Cell Source Type: research
High efficient differentiation of human adipose-derived stem cells into retinal pigment epithelium-like cells in medium containing small molecules inducers with a simple method.
CONCLUSION: Human adipose stem cells can differentiate into retinal pigmented epithelium cells, which can be used in cell replacement therapy for degenerative diseases including age-related macular degeneration (AMD) as well as retinitis pigmentosa (RP). PMID: 30736904 [PubMed - in process] (Source: Tissue and Cell)
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Aboutaleb Kadkhodaeian H, Salati A, Lashay A Tags: Tissue Cell Source Type: research
Multilineage potential research on pancreatic mesenchymal stem cells of bovine.
In this study, pancreatic was collected to isolate MSCs from bovine, and then their biological characteristics such as growth kinetics, surface antigen, and multilineage potential were examined. Pancreatic MSCs of bovine (B-PMSCs) could be cultured for 65 passages in vitro. Growth kinetics analyses indicated that B-PMSCs had a strong capacity for self-renewal in vitro and their proliferation capacity appeared to decrease by passaging. Surface antigen detection showed that B-PMSCs expressed CD29, CD44, CD73, CD90, CD106, CD166, Vimentin, Nestin and Insulin, but not expressed CD34 and CD45. Furthermore, B-PMSCs could be indu...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Gao F, Wu Y, Wen H, Zhu W, Ren H, Guan W, Tian X Tags: Tissue Cell Source Type: research
Morphology of nutrient storage cells in the gonadal area of the Pacific oyster, Crassostrea gigas (Thunberg, 1793).
Abstract Successful gametogenesis in invertebrates is tightly associated with functioning of specific nutrient-storing cells. In oysters, cells of vesicular connective tissue (VCT-cells), also referred to as storage cells, which form a meshwork around gonadal acini, are the major population of cells that accumulate and provide nutrients for developing gametes. During the annual reproductive cycle, populations of developing germ cells and VCT-cells demonstrate the inversely proportional size dynamics: the larger the acini, the smaller the VCT-cells. In the present study, the morphology of VCT-cells in the Pacific o...
Source: Tissue and Cell - February 1, 2019 Category: Cytology Authors: Yurchenko OV, Kalachev AV Tags: Tissue Cell Source Type: research