SIAH1 promotes senescence and apoptosis of nucleus pulposus cells to exacerbate disc degeneration through ubiquitinating XIAP
CONCLUSION: In IDD patients, SIAH1 can target and ubiquitinate XIAP, thereby mediating senescence, apoptosis, increased inflammation, and decreased ECM synthesis of NPCs, while METTL3-mediated m6A can methylate SIAH1 mRNA, producing harmful effects.PMID:35580525 | DOI:10.1016/j.tice.2022.101820 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shibing Fang Fangjun Zeng Rongchun Chen Mingheng Li Source Type: research

Decellularization of human amniotic membrane using detergent-free methods: Possibilities in tissue engineering
In conclusion, the present study showed that the use of detergent-free protocols is applicable for the decellularization of HAM, and the obtained tissues may be considered as inexpensive dressings for numerous tissue engineering applications.PMID:35580526 | DOI:10.1016/j.tice.2022.101818 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shahrzad Ashouri Seyede Atefe Hosseini Seyed Javad Hoseini Fatemeh Tara Alireza Ebrahimzadeh-Bideskan Thomas J Webster Saeid Kargozar Source Type: research

SIAH1 promotes senescence and apoptosis of nucleus pulposus cells to exacerbate disc degeneration through ubiquitinating XIAP
CONCLUSION: In IDD patients, SIAH1 can target and ubiquitinate XIAP, thereby mediating senescence, apoptosis, increased inflammation, and decreased ECM synthesis of NPCs, while METTL3-mediated m6A can methylate SIAH1 mRNA, producing harmful effects.PMID:35580525 | DOI:10.1016/j.tice.2022.101820 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shibing Fang Fangjun Zeng Rongchun Chen Mingheng Li Source Type: research

Decellularization of human amniotic membrane using detergent-free methods: Possibilities in tissue engineering
In conclusion, the present study showed that the use of detergent-free protocols is applicable for the decellularization of HAM, and the obtained tissues may be considered as inexpensive dressings for numerous tissue engineering applications.PMID:35580526 | DOI:10.1016/j.tice.2022.101818 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shahrzad Ashouri Seyede Atefe Hosseini Seyed Javad Hoseini Fatemeh Tara Alireza Ebrahimzadeh-Bideskan Thomas J Webster Saeid Kargozar Source Type: research

SIAH1 promotes senescence and apoptosis of nucleus pulposus cells to exacerbate disc degeneration through ubiquitinating XIAP
CONCLUSION: In IDD patients, SIAH1 can target and ubiquitinate XIAP, thereby mediating senescence, apoptosis, increased inflammation, and decreased ECM synthesis of NPCs, while METTL3-mediated m6A can methylate SIAH1 mRNA, producing harmful effects.PMID:35580525 | DOI:10.1016/j.tice.2022.101820 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shibing Fang Fangjun Zeng Rongchun Chen Mingheng Li Source Type: research

Decellularization of human amniotic membrane using detergent-free methods: Possibilities in tissue engineering
In conclusion, the present study showed that the use of detergent-free protocols is applicable for the decellularization of HAM, and the obtained tissues may be considered as inexpensive dressings for numerous tissue engineering applications.PMID:35580526 | DOI:10.1016/j.tice.2022.101818 (Source: Tissue and Cell)
Source: Tissue and Cell - May 17, 2022 Category: Cytology Authors: Shahrzad Ashouri Seyede Atefe Hosseini Seyed Javad Hoseini Fatemeh Tara Alireza Ebrahimzadeh-Bideskan Thomas J Webster Saeid Kargozar Source Type: research

Automatic microscopic diagnosis of diseases using an improved UNet++ architecture
Tissue Cell. 2022 May 7;76:101816. doi: 10.1016/j.tice.2022.101816. Online ahead of print.ABSTRACTAnthrax is a severe infectious disease caused by the Bacillus anthracis bacterium. This paper aims to design and implement a fast and reliable system based on microscopic image processing of patient tissue samples for the automatic diagnosis of anthrax and other tissues diseases, metastasis detection, patient prognosis, etc. An improved UNet++ architecture is proposed to segment microscopic images of patient tissue samples. The proposed model combines multi-scale features by adding skip connections in two paths; the forward pa...
Source: Tissue and Cell - May 15, 2022 Category: Cytology Authors: Fatemeh Hoorali Hossein Khosravi Bagher Moradi Source Type: research

Automatic microscopic diagnosis of diseases using an improved UNet++ architecture
Tissue Cell. 2022 May 7;76:101816. doi: 10.1016/j.tice.2022.101816. Online ahead of print.ABSTRACTAnthrax is a severe infectious disease caused by the Bacillus anthracis bacterium. This paper aims to design and implement a fast and reliable system based on microscopic image processing of patient tissue samples for the automatic diagnosis of anthrax and other tissues diseases, metastasis detection, patient prognosis, etc. An improved UNet++ architecture is proposed to segment microscopic images of patient tissue samples. The proposed model combines multi-scale features by adding skip connections in two paths; the forward pa...
Source: Tissue and Cell - May 15, 2022 Category: Cytology Authors: Fatemeh Hoorali Hossein Khosravi Bagher Moradi Source Type: research

Ultrasound microbubble-mediated miR-503-5p downregulation suppressed in vitro CRC progression via promoting SALL1 expression
CONCLUSION: Ultrasound microbubble-mediated miR-503-5p downregulation suppressed in vitro CRC progression via promoting SALL1 expression.PMID:35567907 | DOI:10.1016/j.tice.2022.101811 (Source: Tissue and Cell)
Source: Tissue and Cell - May 14, 2022 Category: Cytology Authors: Yaru Wang Yanting Li Xin Li Source Type: research

Ultrasound microbubble-mediated miR-503-5p downregulation suppressed in vitro CRC progression via promoting SALL1 expression
CONCLUSION: Ultrasound microbubble-mediated miR-503-5p downregulation suppressed in vitro CRC progression via promoting SALL1 expression.PMID:35567907 | DOI:10.1016/j.tice.2022.101811 (Source: Tissue and Cell)
Source: Tissue and Cell - May 14, 2022 Category: Cytology Authors: Yaru Wang Yanting Li Xin Li Source Type: research

Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3 β-dependent manner
Tissue Cell. 2022 May 2;76:101813. doi: 10.1016/j.tice.2022.101813. Online ahead of print.ABSTRACTBromodomain-containing protein 7 (BRD7) is linked to a variety of pathophysiological conditions. However, it is still unclear whether BRD7 is connected with diabetic nephropathy. This research explored the relevance of BRD7 in diabetic nephropathy using high glucose (HG)-stimulated podocytes in vitro. BRD7 expression in podocytes was decreased after HG stimulation. Podocytes with forced BRD7 expression were protected from HG-induced apoptosis, oxidative stress and inflammation. Further data revealed that forced expression of B...
Source: Tissue and Cell - May 13, 2022 Category: Cytology Authors: Xiangyou Yu Ning Jiang Jing Li Xiaofeng Li Shenglin He Source Type: research

Functional morphology of the seminal receptacle of Mithrax, Mithraculus and Omalacantha spider crabs (Brachyura: Mithracidae)
Tissue Cell. 2022 Apr 27;76:101806. doi: 10.1016/j.tice.2022.101806. Online ahead of print.ABSTRACTMost Majoidea crabs display high sperm competition rates due to the development of spermatic strata within the seminal receptacle (SR). To verify the occurrence of sperm competition, SR anatomy and histochemistry analyses were performed in Mithrax hispidus, Omalacantha bicornuta, and Mithraculus forceps. The SR of the three Mithracidae species is classified into two regions, one of mesodermal origin (dorsal), consisting of a stratified epithelium with desquamation cells that produce a holocrine secretion, and the other, an ec...
Source: Tissue and Cell - May 13, 2022 Category: Cytology Authors: C O Assugeni F J Zara Source Type: research

Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3 β-dependent manner
Tissue Cell. 2022 May 2;76:101813. doi: 10.1016/j.tice.2022.101813. Online ahead of print.ABSTRACTBromodomain-containing protein 7 (BRD7) is linked to a variety of pathophysiological conditions. However, it is still unclear whether BRD7 is connected with diabetic nephropathy. This research explored the relevance of BRD7 in diabetic nephropathy using high glucose (HG)-stimulated podocytes in vitro. BRD7 expression in podocytes was decreased after HG stimulation. Podocytes with forced BRD7 expression were protected from HG-induced apoptosis, oxidative stress and inflammation. Further data revealed that forced expression of B...
Source: Tissue and Cell - May 13, 2022 Category: Cytology Authors: Xiangyou Yu Ning Jiang Jing Li Xiaofeng Li Shenglin He Source Type: research

Extracellular vesicles from blood of breast cancer women induce angiogenic processes in HUVECs
In this study we studied whether EV fractions deprived of platelet EVs from breast cancer women (BC EVs) can mediate cell processes related with angiogenesis in human umbilical vein endothelial cells (HUVECs). Our findings demonstrate that BC EVs enhance migration, invasion and formation of new tubules in HUVECs, compared with EV fractions deprived of platelet EVs from healthy women (Ctrl EVs). In summary, we demonstrate, for the first time, that BC EVs induce cellular processes in HUVECs that participate in angiogenesis.PMID:35526310 | DOI:10.1016/j.tice.2022.101814 (Source: Tissue and Cell)
Source: Tissue and Cell - May 8, 2022 Category: Cytology Authors: Alejandra Garcia-Hernandez Emmanuel Reyes-Uribe Claudia Arce-Salinas Karen-Griselda de la Cruz-Lopez Joaquin Manzo-Merino Ana-Laura Guzman-Ortiz Hector Quezada Pedro Cortes-Reynosa Fernando Breton-Mora Irma Elizalde-Acosta Rocio Thompson-Bonilla Eduardo P Source Type: research

Conditioned medium from amniotic fluid mesenchymal stem cells could modulate Alzheimer's disease-like changes in human neuroblastoma cell line SY-SY5Y in a paracrine manner
CONCLUSION: Amniotic fluid stem cell secretion reduces AD-like pathologies in the human neuronal lineage.PMID:35526311 | DOI:10.1016/j.tice.2022.101808 (Source: Tissue and Cell)
Source: Tissue and Cell - May 8, 2022 Category: Cytology Authors: Milad Hasanpour Reza Rahbarghazi Alireza Nourazarian Fatemeh Khaki-Khatibi Çigir Biray Avci Mehdi Hassanpour Mehdi Talebi Hossein Taghavi Leila Salimi Source Type: research