In vivo study on osteogenic efficiency of nHA/ gel porous scaffold with nacre water-soluble matrix
CONCLUSION: WSM promotes osteoblast differentiation and bone mineralization. The results confirm that the nHA/ Gel Porous Scaffold with Nacre Water-Soluble Matrix has a significant bone promoting effect and can be used as a choice for tissue engineering to repair bone defects.PMID:38489914 | DOI:10.1016/j.tice.2024.102347 (Source: Tissue and Cell)
Source: Tissue and Cell - March 15, 2024 Category: Cytology Authors: SiRi-GuLeng Li Zhu-Ling Guo Si-Yu Tao Tao Han Jie Zhou Wan-Yun Lin Xiang Guo Chu-Xing Li Sunchuri Diwas Xiao-Wen Hu Source Type: research
Propofol modulates Nrf2/NLRP3 signaling to ameliorate cigarette smoke-induced damage in human bronchial epithelial cells
This study aimed to elucidate the protective mechanisms of propofol against CSE-induced damage in human bronchial epithelial 16HBE cells. In CSE-induced 16HBE cells treated by propofol with or without transfection of nuclear factor erythroid 2-related factor 2 (Nrf2) interference plasmids, CCK-8 assay and lactate dehydrogenase (LDH) assay evaluated cytotoxicity. TUNEL assay and Western blot appraised cell apoptosis. ELISA and relevant assay kits severally measured inflammatory and oxidative stress levels. DCFH-DA fluorescent probe detected intracellular reactive oxygen species (ROS) activity. Immunofluorescence staining an...
Source: Tissue and Cell - March 13, 2024 Category: Cytology Authors: Linmei Xu Yanbin Wang Qumin Chen Xiaolei Zhu Jiageng Hong Source Type: research
Propofol modulates Nrf2/NLRP3 signaling to ameliorate cigarette smoke-induced damage in human bronchial epithelial cells
This study aimed to elucidate the protective mechanisms of propofol against CSE-induced damage in human bronchial epithelial 16HBE cells. In CSE-induced 16HBE cells treated by propofol with or without transfection of nuclear factor erythroid 2-related factor 2 (Nrf2) interference plasmids, CCK-8 assay and lactate dehydrogenase (LDH) assay evaluated cytotoxicity. TUNEL assay and Western blot appraised cell apoptosis. ELISA and relevant assay kits severally measured inflammatory and oxidative stress levels. DCFH-DA fluorescent probe detected intracellular reactive oxygen species (ROS) activity. Immunofluorescence staining an...
Source: Tissue and Cell - March 13, 2024 Category: Cytology Authors: Linmei Xu Yanbin Wang Qumin Chen Xiaolei Zhu Jiageng Hong Source Type: research
Propofol modulates Nrf2/NLRP3 signaling to ameliorate cigarette smoke-induced damage in human bronchial epithelial cells
This study aimed to elucidate the protective mechanisms of propofol against CSE-induced damage in human bronchial epithelial 16HBE cells. In CSE-induced 16HBE cells treated by propofol with or without transfection of nuclear factor erythroid 2-related factor 2 (Nrf2) interference plasmids, CCK-8 assay and lactate dehydrogenase (LDH) assay evaluated cytotoxicity. TUNEL assay and Western blot appraised cell apoptosis. ELISA and relevant assay kits severally measured inflammatory and oxidative stress levels. DCFH-DA fluorescent probe detected intracellular reactive oxygen species (ROS) activity. Immunofluorescence staining an...
Source: Tissue and Cell - March 13, 2024 Category: Cytology Authors: Linmei Xu Yanbin Wang Qumin Chen Xiaolei Zhu Jiageng Hong Source Type: research
STUB1 increases adiponectin expression by inducing ubiquitination and degradation of NR2F2, thereby reducing hepatic stellate cell activation and alleviating non-alcoholic fatty liver disease
CONCLUSION: This study highlights the role of STUB1 in reducing HSC activation and alleviating NAFLD by attenuating NR2F2-mediated transcriptional repression of APN.PMID:38471267 | DOI:10.1016/j.tice.2024.102345 (Source: Tissue and Cell)
Source: Tissue and Cell - March 12, 2024 Category: Cytology Authors: Zheng Mao Jindong Zhao Fan Cui Zhen Li Jinjin Cao Jingjing Zhou Mingliang Hou Zengkun Qian Source Type: research
STUB1 increases adiponectin expression by inducing ubiquitination and degradation of NR2F2, thereby reducing hepatic stellate cell activation and alleviating non-alcoholic fatty liver disease
CONCLUSION: This study highlights the role of STUB1 in reducing HSC activation and alleviating NAFLD by attenuating NR2F2-mediated transcriptional repression of APN.PMID:38471267 | DOI:10.1016/j.tice.2024.102345 (Source: Tissue and Cell)
Source: Tissue and Cell - March 12, 2024 Category: Cytology Authors: Zheng Mao Jindong Zhao Fan Cui Zhen Li Jinjin Cao Jingjing Zhou Mingliang Hou Zengkun Qian Source Type: research
Human buccal epithelial cells as a model system for molecular analysis of DNA, RNA and protein
We report use of human buccal epithelial cells as an easy model system for isolation and molecular analysis of genomic DNA, RNA, and protein to study any gene of interest by Polymerase Chain Reaction (PCR), RNA expression by Reverse Transcription-PCR (RT-PCR), protein-profiling, and expression by western blot as well as DNA-methylation by Msp I/Hpa II-restriction digestion. We used simple methods to isolate genomic DNA, RNA and protein from human buccal cells collected by oral swab and cultured them in-vitro. The microscopic observation of haematoxylin and eosin (EA-50) stained cells, genomic PCR of house-keeping genes (GA...
Source: Tissue and Cell - March 10, 2024 Category: Cytology Authors: Ajay Kumar Danga Pramod C Rath Source Type: research
Human buccal epithelial cells as a model system for molecular analysis of DNA, RNA and protein
We report use of human buccal epithelial cells as an easy model system for isolation and molecular analysis of genomic DNA, RNA, and protein to study any gene of interest by Polymerase Chain Reaction (PCR), RNA expression by Reverse Transcription-PCR (RT-PCR), protein-profiling, and expression by western blot as well as DNA-methylation by Msp I/Hpa II-restriction digestion. We used simple methods to isolate genomic DNA, RNA and protein from human buccal cells collected by oral swab and cultured them in-vitro. The microscopic observation of haematoxylin and eosin (EA-50) stained cells, genomic PCR of house-keeping genes (GA...
Source: Tissue and Cell - March 10, 2024 Category: Cytology Authors: Ajay Kumar Danga Pramod C Rath Source Type: research
Therapeutic effect of human umbilical cord mesenchymal stem cells and their conditioned medium on LPS-induced endometritis in mice
CONCLUSIONS: Intrauterine infusion of hUC-MSCs and MSC-CM can alleviate LPS induced endometritis.PMID:38460354 | DOI:10.1016/j.tice.2024.102346 (Source: Tissue and Cell)
Source: Tissue and Cell - March 9, 2024 Category: Cytology Authors: Jingyi Li Xiaodi Yin Ming Du Caiyi Wang Feng Zou Jun Ma Yuxia Song Source Type: research
Therapeutic effect of human umbilical cord mesenchymal stem cells and their conditioned medium on LPS-induced endometritis in mice
CONCLUSIONS: Intrauterine infusion of hUC-MSCs and MSC-CM can alleviate LPS induced endometritis.PMID:38460354 | DOI:10.1016/j.tice.2024.102346 (Source: Tissue and Cell)
Source: Tissue and Cell - March 9, 2024 Category: Cytology Authors: Jingyi Li Xiaodi Yin Ming Du Caiyi Wang Feng Zou Jun Ma Yuxia Song Source Type: research
Effects of endoplasmic reticulum stress on chondrocyte apoptosis via the PI3K/AKT signaling pathway
Tissue Cell. 2024 Feb 25;87:102340. doi: 10.1016/j.tice.2024.102340. Online ahead of print.ABSTRACTChondrocytes undergo endoplasmic reticulum stress (ERS)-induced apoptosis under abnormal stimulation. However, the underlying molecular mechanism remains unclear. We investigated the regulatory effect of the PI3K/AKT signaling pathway on ERS and its effect on chondrocyte apoptosis. In addition, we established a unilateral anterior crossbite (UAC) model in rats to induce temporomandibular joint osteoarthritis (TMJOA). Chondrocytes were isolated from the temporomandibular joints and treated with lipopolysaccharide (LPS) in vitr...
Source: Tissue and Cell - March 6, 2024 Category: Cytology Authors: Libo Zhou Fan Wu Jing Wang Yuqing Zhao Gaoyi Wu Yucheng Su Source Type: research
Effects of endoplasmic reticulum stress on chondrocyte apoptosis via the PI3K/AKT signaling pathway
Tissue Cell. 2024 Feb 25;87:102340. doi: 10.1016/j.tice.2024.102340. Online ahead of print.ABSTRACTChondrocytes undergo endoplasmic reticulum stress (ERS)-induced apoptosis under abnormal stimulation. However, the underlying molecular mechanism remains unclear. We investigated the regulatory effect of the PI3K/AKT signaling pathway on ERS and its effect on chondrocyte apoptosis. In addition, we established a unilateral anterior crossbite (UAC) model in rats to induce temporomandibular joint osteoarthritis (TMJOA). Chondrocytes were isolated from the temporomandibular joints and treated with lipopolysaccharide (LPS) in vitr...
Source: Tissue and Cell - March 6, 2024 Category: Cytology Authors: Libo Zhou Fan Wu Jing Wang Yuqing Zhao Gaoyi Wu Yucheng Su Source Type: research
Effects of endoplasmic reticulum stress on chondrocyte apoptosis via the PI3K/AKT signaling pathway
Tissue Cell. 2024 Feb 25;87:102340. doi: 10.1016/j.tice.2024.102340. Online ahead of print.ABSTRACTChondrocytes undergo endoplasmic reticulum stress (ERS)-induced apoptosis under abnormal stimulation. However, the underlying molecular mechanism remains unclear. We investigated the regulatory effect of the PI3K/AKT signaling pathway on ERS and its effect on chondrocyte apoptosis. In addition, we established a unilateral anterior crossbite (UAC) model in rats to induce temporomandibular joint osteoarthritis (TMJOA). Chondrocytes were isolated from the temporomandibular joints and treated with lipopolysaccharide (LPS) in vitr...
Source: Tissue and Cell - March 6, 2024 Category: Cytology Authors: Libo Zhou Fan Wu Jing Wang Yuqing Zhao Gaoyi Wu Yucheng Su Source Type: research
D-beta-hydroxybutyrate up-regulates Claudin-1 and alleviates the intestinal hyperpermeability in lipopolysaccharide-treated mice
In conclusion, BHB may alleviate the hyperpermeability of intestinal epithelium via up-regulation of Claudin-1 in colon in LPS-treated mice.PMID:38442546 | DOI:10.1016/j.tice.2024.102343 (Source: Tissue and Cell)
Source: Tissue and Cell - March 5, 2024 Category: Cytology Authors: Ting Wang Yuchen Zhuang Chenglong Yu Zhaobo Wang Yuan Liu Qian Xu Kun Liu Yanning Li Source Type: research
Hypoxia preconditioning of human amniotic mesenchymal stem cells enhances proliferation and migration and promotes their homing via the HGF/C-MET signaling axis to augment the repair of acute liver failure
CONCLUSIONS: Hypoxic preconditioning depicted no impact on the morphology and phenotype features of the human amniotic mesenchymal stem cells, but it can promote their proliferation, migration, anti-apoptotic effect, and homing rate and improve the repair of acute liver failure, which might be mediated by the HGF/c-Met signaling axis.PMID:38442547 | DOI:10.1016/j.tice.2024.102326 (Source: Tissue and Cell)
Source: Tissue and Cell - March 5, 2024 Category: Cytology Authors: Qian Wang Yuwen Li Hui Yuan Linya Peng Zixing Dai Ye Sun Rui Liu Wenting Li Jun Li Chuanlong Zhu Source Type: research
