In vitro biocompatibility of ammonia-free silver fluoride products on human dental pulp stem cells
This study aimed to compare the biological properties of two commercial SF products, one of them with ammonium (Riva Star; SDF) and the other ammonium-free (Riva Star Aqua; AgF), both with or without potassium iodide (KI), by the assessment of the cytotoxicity of the materials' eluates.METHODS: Human dental pulp stem cells (hDPSCs) were obtained from healthy 18-23-year-old donors. Three dilutions were prepared for the tested materials (0.005%, 0.0005%, and 0.0001%). The following groups were assessed: (AgF, AgF+KI, SDF, SDF+KI, KI, negative control). A series of cytocompatibility assays were performed: MTT assay, IC50 assa...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Sergio L ópez-García Jos é Luis Sanz Ricardo E O ñate-Sánchez Leopoldo Forner David Garc ía-Bernal Laura Murcia Francisco J Rodr íguez-Lozano Carmen Llena Source Type: research
VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis
This study aims to explore the role and the potential mechanism of VSIG4 in IBD. Clinical samples were obtained from IBD patients and were examined by immunohistochemical staining. THP-1 cells were differentiated into macrophages, and then stimulated with IL-4 plus IL-13 or LPS to induce pro-inflammatory (M1) or anti-inflammatory (M2) phenotype. Cell transfection was conducted to overexpress VSIG4. Western blot and immunofluorescence assays were performed to assess NLRP3 inflammasome- and pyroptosis-related proteins. Cytokines were measured using ELISA. A cell co-culture model of Caco-2 cells and VSIG4-mediated macrophages...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Yuan Liao Chaonan Deng Xichuan Wang Source Type: research
In vitro biocompatibility of ammonia-free silver fluoride products on human dental pulp stem cells
This study aimed to compare the biological properties of two commercial SF products, one of them with ammonium (Riva Star; SDF) and the other ammonium-free (Riva Star Aqua; AgF), both with or without potassium iodide (KI), by the assessment of the cytotoxicity of the materials' eluates.METHODS: Human dental pulp stem cells (hDPSCs) were obtained from healthy 18-23-year-old donors. Three dilutions were prepared for the tested materials (0.005%, 0.0005%, and 0.0001%). The following groups were assessed: (AgF, AgF+KI, SDF, SDF+KI, KI, negative control). A series of cytocompatibility assays were performed: MTT assay, IC50 assa...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Sergio L ópez-García Jos é Luis Sanz Ricardo E O ñate-Sánchez Leopoldo Forner David Garc ía-Bernal Laura Murcia Francisco J Rodr íguez-Lozano Carmen Llena Source Type: research
Transient receptor potential channel TRPM4 favors oxidized low-density lipoprotein-induced coronary endothelial cell dysfunction via a mechanism involving ferroptosis
This study aimed to reveal the potential role of TRPM4 in AS along with the mechanism. Human coronary artery endothelial cells (HCAECs) induced by ox-LDL was regarded as an in vitro model. The impacts of TRPM4 knockdown on cellular inflammation response, oxidative stress, normal endothelial function and lipid peroxidation were evaluated. Given that ferroptosis promotes AS progression, the effects of TRPM4 on intracellular iron ions and ferroptosis-related proteins was determined. Afterwards, HCAECs were treated with ferroptosis inducer erastin, and the influence of ferroptosis in the cellular model was revealed. TRPM4 was ...
Source: Tissue and Cell - December 16, 2023 Category: Cytology Authors: Fengxiang Ye Dongtao Liu Junjie Zhang Source Type: research
Transient receptor potential channel TRPM4 favors oxidized low-density lipoprotein-induced coronary endothelial cell dysfunction via a mechanism involving ferroptosis
This study aimed to reveal the potential role of TRPM4 in AS along with the mechanism. Human coronary artery endothelial cells (HCAECs) induced by ox-LDL was regarded as an in vitro model. The impacts of TRPM4 knockdown on cellular inflammation response, oxidative stress, normal endothelial function and lipid peroxidation were evaluated. Given that ferroptosis promotes AS progression, the effects of TRPM4 on intracellular iron ions and ferroptosis-related proteins was determined. Afterwards, HCAECs were treated with ferroptosis inducer erastin, and the influence of ferroptosis in the cellular model was revealed. TRPM4 was ...
Source: Tissue and Cell - December 16, 2023 Category: Cytology Authors: Fengxiang Ye Dongtao Liu Junjie Zhang Source Type: research
Tertiary lymphoid structures, a historical reappraisal
Tissue Cell. 2023 Dec 10;86:102288. doi: 10.1016/j.tice.2023.102288. Online ahead of print.ABSTRACTTertiary lymphoid structures (TLSs) are accumulations of lymphoid cells within non-lymphoid organs that share the cellular compartments, spatial organization, vasculature, chemokines, and function with secondary lymphoid organs, especially lymph nodes. TLSs are organized into a separate T cell and B cell compartments which contain germinal centers with follicular dendritic cells. In most cases, TLSs contain Peripheral Node addressin (PNAD) expressing high endothelial venules (HEVs). TLSs have been described in various mouse m...
Source: Tissue and Cell - December 15, 2023 Category: Cytology Authors: Domenico Ribatti Source Type: research
Tertiary lymphoid structures, a historical reappraisal
Tissue Cell. 2023 Dec 10;86:102288. doi: 10.1016/j.tice.2023.102288. Online ahead of print.ABSTRACTTertiary lymphoid structures (TLSs) are accumulations of lymphoid cells within non-lymphoid organs that share the cellular compartments, spatial organization, vasculature, chemokines, and function with secondary lymphoid organs, especially lymph nodes. TLSs are organized into a separate T cell and B cell compartments which contain germinal centers with follicular dendritic cells. In most cases, TLSs contain Peripheral Node addressin (PNAD) expressing high endothelial venules (HEVs). TLSs have been described in various mouse m...
Source: Tissue and Cell - December 15, 2023 Category: Cytology Authors: Domenico Ribatti Source Type: research
Zic family member 3 attenuates oxidative stress-induced vascular smooth muscle cell apoptosis in patients with chronic kidney disease
Tissue Cell. 2023 Dec 8;86:102286. doi: 10.1016/j.tice.2023.102286. Online ahead of print.ABSTRACTNeointimal hyperplasia is reportedly essential for arteriovenous fistulas (AVF) in patients undergoing hemodialysis. Oxidative stress is vital in the progression of uremic venous intimal hyperplasia. Studies have suggested that zinc ions obstruct vascular calcification in patients with chronic kidney disease (CKD). Recent studies have shown that the zinc finger protein, Zic family member 3 (ZIC3), is crucial for the earliest cardiovascular progenitors. ZIC3 mutations are associated with congenital heart disease. However, the m...
Source: Tissue and Cell - December 13, 2023 Category: Cytology Authors: Jianya Gao Lei Liu Zecheng Wu Hua Gan Source Type: research
Zic family member 3 attenuates oxidative stress-induced vascular smooth muscle cell apoptosis in patients with chronic kidney disease
Tissue Cell. 2023 Dec 8;86:102286. doi: 10.1016/j.tice.2023.102286. Online ahead of print.ABSTRACTNeointimal hyperplasia is reportedly essential for arteriovenous fistulas (AVF) in patients undergoing hemodialysis. Oxidative stress is vital in the progression of uremic venous intimal hyperplasia. Studies have suggested that zinc ions obstruct vascular calcification in patients with chronic kidney disease (CKD). Recent studies have shown that the zinc finger protein, Zic family member 3 (ZIC3), is crucial for the earliest cardiovascular progenitors. ZIC3 mutations are associated with congenital heart disease. However, the m...
Source: Tissue and Cell - December 13, 2023 Category: Cytology Authors: Jianya Gao Lei Liu Zecheng Wu Hua Gan Source Type: research
Zic family member 3 attenuates oxidative stress-induced vascular smooth muscle cell apoptosis in patients with chronic kidney disease
Tissue Cell. 2023 Dec 8;86:102286. doi: 10.1016/j.tice.2023.102286. Online ahead of print.ABSTRACTNeointimal hyperplasia is reportedly essential for arteriovenous fistulas (AVF) in patients undergoing hemodialysis. Oxidative stress is vital in the progression of uremic venous intimal hyperplasia. Studies have suggested that zinc ions obstruct vascular calcification in patients with chronic kidney disease (CKD). Recent studies have shown that the zinc finger protein, Zic family member 3 (ZIC3), is crucial for the earliest cardiovascular progenitors. ZIC3 mutations are associated with congenital heart disease. However, the m...
Source: Tissue and Cell - December 13, 2023 Category: Cytology Authors: Jianya Gao Lei Liu Zecheng Wu Hua Gan Source Type: research
Emodin suppresses adipogenesis of bone marrow derived mesenchymal stem cells from aplastic anemia via increasing TRIB3 expression
CONCLUSION: Our findings propose that emodin mediated TRIB3 up-regulation alleviates the adipogenic capacity of AA BM-MSCs, and emodin could serve as a potential therapeutic regimen for AA therapy.PMID:38086146 | DOI:10.1016/j.tice.2023.102287 (Source: Tissue and Cell)
Source: Tissue and Cell - December 12, 2023 Category: Cytology Authors: Xianning Zhang Lulu Liu Jian Wang Mingkang Yao Lei Liu Haihui Liu Saisai Ren Peng Wei Panpan Cheng Xiyu Li Hao Zhang Mingtai Chen Source Type: research
Emodin suppresses adipogenesis of bone marrow derived mesenchymal stem cells from aplastic anemia via increasing TRIB3 expression
CONCLUSION: Our findings propose that emodin mediated TRIB3 up-regulation alleviates the adipogenic capacity of AA BM-MSCs, and emodin could serve as a potential therapeutic regimen for AA therapy.PMID:38086146 | DOI:10.1016/j.tice.2023.102287 (Source: Tissue and Cell)
Source: Tissue and Cell - December 12, 2023 Category: Cytology Authors: Xianning Zhang Lulu Liu Jian Wang Mingkang Yao Lei Liu Haihui Liu Saisai Ren Peng Wei Panpan Cheng Xiyu Li Hao Zhang Mingtai Chen Source Type: research
A review of the therapeutic potential of dental stem cells as scaffold-free models for tissue engineering application
This study delves into the latest advancements in leveraging dental stem cells and scaffold-free techniques for the regeneration of various tissues. This paper offers a comprehensive summary of recent developments in the utilization of dental stem cells and scaffold-free methods for tissue generation. It explores the potential of these approaches to advance tissue engineering and their effectiveness in therapies aimed at tissue regeneration.PMID:38070384 | DOI:10.1016/j.tice.2023.102281 (Source: Tissue and Cell)
Source: Tissue and Cell - December 9, 2023 Category: Cytology Authors: Elaheh Dalir Abdolahinia Shayan Golestani Sepideh Seif Narges Afra Khotan Aflatoonian Ali Jalalian Nasrin Valizadeh Elham Dalir Abdollahinia Source Type: research
A review of the therapeutic potential of dental stem cells as scaffold-free models for tissue engineering application
This study delves into the latest advancements in leveraging dental stem cells and scaffold-free techniques for the regeneration of various tissues. This paper offers a comprehensive summary of recent developments in the utilization of dental stem cells and scaffold-free methods for tissue generation. It explores the potential of these approaches to advance tissue engineering and their effectiveness in therapies aimed at tissue regeneration.PMID:38070384 | DOI:10.1016/j.tice.2023.102281 (Source: Tissue and Cell)
Source: Tissue and Cell - December 9, 2023 Category: Cytology Authors: Elaheh Dalir Abdolahinia Shayan Golestani Sepideh Seif Narges Afra Khotan Aflatoonian Ali Jalalian Nasrin Valizadeh Elham Dalir Abdollahinia Source Type: research
The E3 ubiquitin ligase RNF135 modulates chemotherapy resistance to oxaliplatin for colorectal cancer by modulating autophagy
Tissue Cell. 2023 Nov 30;86:102282. doi: 10.1016/j.tice.2023.102282. Online ahead of print.ABSTRACTBACKGROUND: RING finger protein 135 plays an important role in tumorigenesis and is associated with drug resistance.METHODS: Bioinformatics analysis showed that RNF135 was significantly differentially expressed in colorectal cancer. RT-qPCR and western blot were used to detect the expression of RNF135. Immunohistochemical analysis were used to measure the expression of RNF135 and Ki-67.RESULTS: The expression of RNF135 was up-regulated in human tissue samples and colorectal cancer and was positively correlated with Ki-67. Com...
Source: Tissue and Cell - December 6, 2023 Category: Cytology Authors: Zhen Qiu Shuyan He Boyi Lu Yuejun Sun Ting Zhang Wei Lv Dong Shen Source Type: research
