Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
Elucidating the mechanism of IL-1 β-Mediated Piezo1 expression regulation of chondrocyte autophagy and apoptosis via the PI3K/AKT/mTOR signaling Pathway
Tissue Cell. 2023 Dec 19;86:102291. doi: 10.1016/j.tice.2023.102291. Online ahead of print.ABSTRACTFor the pathogenesis of osteoarthritis (OA), the classical view is that chondrocyte apoptosis is associated with and may cause age-related joint degeneration. Recent observations indicate that Piezo1, a mechanical stress channel expressed in articular cartilage, plays a crucial role in this process. We wanted to investigate whether other conditions activate the expression of Piezo1 in chondrocytes. Therefore, we simulated OA to investigate whether Piezo1 gene expression and channel function were affected by the inflammatory f...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Wanzhuo Chen Haining Zhang Source Type: research
Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
Elucidating the mechanism of IL-1 β-Mediated Piezo1 expression regulation of chondrocyte autophagy and apoptosis via the PI3K/AKT/mTOR signaling Pathway
Tissue Cell. 2023 Dec 19;86:102291. doi: 10.1016/j.tice.2023.102291. Online ahead of print.ABSTRACTFor the pathogenesis of osteoarthritis (OA), the classical view is that chondrocyte apoptosis is associated with and may cause age-related joint degeneration. Recent observations indicate that Piezo1, a mechanical stress channel expressed in articular cartilage, plays a crucial role in this process. We wanted to investigate whether other conditions activate the expression of Piezo1 in chondrocytes. Therefore, we simulated OA to investigate whether Piezo1 gene expression and channel function were affected by the inflammatory f...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Wanzhuo Chen Haining Zhang Source Type: research
Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
Elucidating the mechanism of IL-1 β-Mediated Piezo1 expression regulation of chondrocyte autophagy and apoptosis via the PI3K/AKT/mTOR signaling Pathway
Tissue Cell. 2023 Dec 19;86:102291. doi: 10.1016/j.tice.2023.102291. Online ahead of print.ABSTRACTFor the pathogenesis of osteoarthritis (OA), the classical view is that chondrocyte apoptosis is associated with and may cause age-related joint degeneration. Recent observations indicate that Piezo1, a mechanical stress channel expressed in articular cartilage, plays a crucial role in this process. We wanted to investigate whether other conditions activate the expression of Piezo1 in chondrocytes. Therefore, we simulated OA to investigate whether Piezo1 gene expression and channel function were affected by the inflammatory f...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Wanzhuo Chen Haining Zhang Source Type: research
Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
Elucidating the mechanism of IL-1 β-Mediated Piezo1 expression regulation of chondrocyte autophagy and apoptosis via the PI3K/AKT/mTOR signaling Pathway
Tissue Cell. 2023 Dec 19;86:102291. doi: 10.1016/j.tice.2023.102291. Online ahead of print.ABSTRACTFor the pathogenesis of osteoarthritis (OA), the classical view is that chondrocyte apoptosis is associated with and may cause age-related joint degeneration. Recent observations indicate that Piezo1, a mechanical stress channel expressed in articular cartilage, plays a crucial role in this process. We wanted to investigate whether other conditions activate the expression of Piezo1 in chondrocytes. Therefore, we simulated OA to investigate whether Piezo1 gene expression and channel function were affected by the inflammatory f...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Wanzhuo Chen Haining Zhang Source Type: research
Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
Elucidating the mechanism of IL-1 β-Mediated Piezo1 expression regulation of chondrocyte autophagy and apoptosis via the PI3K/AKT/mTOR signaling Pathway
Tissue Cell. 2023 Dec 19;86:102291. doi: 10.1016/j.tice.2023.102291. Online ahead of print.ABSTRACTFor the pathogenesis of osteoarthritis (OA), the classical view is that chondrocyte apoptosis is associated with and may cause age-related joint degeneration. Recent observations indicate that Piezo1, a mechanical stress channel expressed in articular cartilage, plays a crucial role in this process. We wanted to investigate whether other conditions activate the expression of Piezo1 in chondrocytes. Therefore, we simulated OA to investigate whether Piezo1 gene expression and channel function were affected by the inflammatory f...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Wanzhuo Chen Haining Zhang Source Type: research
Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions
This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchym...
Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research
VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis
This study aims to explore the role and the potential mechanism of VSIG4 in IBD. Clinical samples were obtained from IBD patients and were examined by immunohistochemical staining. THP-1 cells were differentiated into macrophages, and then stimulated with IL-4 plus IL-13 or LPS to induce pro-inflammatory (M1) or anti-inflammatory (M2) phenotype. Cell transfection was conducted to overexpress VSIG4. Western blot and immunofluorescence assays were performed to assess NLRP3 inflammasome- and pyroptosis-related proteins. Cytokines were measured using ELISA. A cell co-culture model of Caco-2 cells and VSIG4-mediated macrophages...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Yuan Liao Chaonan Deng Xichuan Wang Source Type: research
In vitro biocompatibility of ammonia-free silver fluoride products on human dental pulp stem cells
This study aimed to compare the biological properties of two commercial SF products, one of them with ammonium (Riva Star; SDF) and the other ammonium-free (Riva Star Aqua; AgF), both with or without potassium iodide (KI), by the assessment of the cytotoxicity of the materials' eluates.METHODS: Human dental pulp stem cells (hDPSCs) were obtained from healthy 18-23-year-old donors. Three dilutions were prepared for the tested materials (0.005%, 0.0005%, and 0.0001%). The following groups were assessed: (AgF, AgF+KI, SDF, SDF+KI, KI, negative control). A series of cytocompatibility assays were performed: MTT assay, IC50 assa...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Sergio L ópez-García Jos é Luis Sanz Ricardo E O ñate-Sánchez Leopoldo Forner David Garc ía-Bernal Laura Murcia Francisco J Rodr íguez-Lozano Carmen Llena Source Type: research
VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis
This study aims to explore the role and the potential mechanism of VSIG4 in IBD. Clinical samples were obtained from IBD patients and were examined by immunohistochemical staining. THP-1 cells were differentiated into macrophages, and then stimulated with IL-4 plus IL-13 or LPS to induce pro-inflammatory (M1) or anti-inflammatory (M2) phenotype. Cell transfection was conducted to overexpress VSIG4. Western blot and immunofluorescence assays were performed to assess NLRP3 inflammasome- and pyroptosis-related proteins. Cytokines were measured using ELISA. A cell co-culture model of Caco-2 cells and VSIG4-mediated macrophages...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Yuan Liao Chaonan Deng Xichuan Wang Source Type: research
In vitro biocompatibility of ammonia-free silver fluoride products on human dental pulp stem cells
This study aimed to compare the biological properties of two commercial SF products, one of them with ammonium (Riva Star; SDF) and the other ammonium-free (Riva Star Aqua; AgF), both with or without potassium iodide (KI), by the assessment of the cytotoxicity of the materials' eluates.METHODS: Human dental pulp stem cells (hDPSCs) were obtained from healthy 18-23-year-old donors. Three dilutions were prepared for the tested materials (0.005%, 0.0005%, and 0.0001%). The following groups were assessed: (AgF, AgF+KI, SDF, SDF+KI, KI, negative control). A series of cytocompatibility assays were performed: MTT assay, IC50 assa...
Source: Tissue and Cell - December 19, 2023 Category: Cytology Authors: Sergio L ópez-García Jos é Luis Sanz Ricardo E O ñate-Sánchez Leopoldo Forner David Garc ía-Bernal Laura Murcia Francisco J Rodr íguez-Lozano Carmen Llena Source Type: research
