The classical D1 dopamine receptor antagonist SCH23390 is a functional sigma-1 receptor allosteric modulator
In this study, we investigated whether SCH23390 functions as an allosteric modulator of Sig1R. We detected increased Sig1R dissociation from binding immunoglobulin protein (BiP) and translocation of Sig1R to the plasma membrane in response to SCH23390 in transfected HEK293T and SH-SY5Y cells, respectively. Activation of Sig1R by SCH23390 was further confirmed by inhibition of GSK3β activity in a time- and dose-dependent manner; this effect was blocked by pretreatment with the Sig1R antagonist, BD1047, and by knockdown of Sig1R. SCH23390 also inhibited GSK3β in wild-type mice but not in Sig1R knockout mice. Finally, we sh...
Source: Acta Pharmacologica Sinica - April 11, 2024 Category: Drugs & Pharmacology Authors: Gu-Fang Zhang Kai-Lian Zhu Qi Li Yue Zhang John L Waddington Xiang-Dong Du Xue-Chu Zhen Source Type: research
Urea promoted soil microbial community and reduced the residual ciprofloxacin in soil and its uptake by Chinese flowering cabbage
In conclusion, moderate urea addition significantly increased soil microbial activity and abundance, which in turn promoted the ciprofloxacin dissipation in soil and plant tissue. The present study provides an economical and operational strategy for the remediation of ciprofloxacin contaminated soils.PMID:38602632 | DOI:10.1007/s11356-024-33213-0 (Source: Environmental Science and Pollution Research International)
Source: Environmental Science and Pollution Research International - April 11, 2024 Category: Environmental Health Authors: Xiaolian Wu Chenze Jin Gengying Du Jianan Wang Jiayi Su Rongxuan Li Source Type: research
Prokaryotic communities of the French Polynesian sponge Dactylospongia metachromia display a site-specific and stable diversity during an aquaculture trial
Antonie Van Leeuwenhoek. 2024 Apr 11;117(1):65. doi: 10.1007/s10482-024-01962-0.ABSTRACTDynamics of microbiomes through time are fundamental regarding survival and resilience of their hosts when facing environmental alterations. As for marine species with commercial applications, such as marine sponges, assessing the temporal change of prokaryotic communities allows us to better consider the adaptation of sponges to aquaculture designs. The present study aims to investigate the factors shaping the microbiome of the sponge Dactylospongia metachromia, in a context of aquaculture development in French Polynesia, Rangiroa, Tua...
Source: Antonie van Leeuwenhoek - April 11, 2024 Category: Microbiology Authors: Mathilde Maslin Beno ît Paix Niels van der Windt Rohani Ambo-Rappe C écile Debitus Nabila Gaertner-Mazouni Raimana Ho Nicole J de Voogd Source Type: research
Talk to your neighbors in an emergency: Stromule-mediated chloroplast-nucleus communication in plant immunity
Curr Opin Plant Biol. 2024 Apr 10;79:102529. doi: 10.1016/j.pbi.2024.102529. Online ahead of print.ABSTRACTHypersensitive response-programmed cell death (HR-PCD) is a response mounted by plants to defend themselves against pathogens. Communication between the chloroplast and the nucleus is critical for the progression of HR-PCD. Tubular protrusions of chloroplasts, known as stromules, are tightly associated with the HR-PCD progression. There is emerging evidence that signaling molecules originating from chloroplasts are transferred to the nucleus through stromules. The translocation of signaling molecules from the chloropl...
Source: Current Opinion in Plant Biology - April 11, 2024 Category: Biology Authors: Seungmee Jung Jongchan Woo Eunsook Park Source Type: research
GPCR kinase subtype requirements for arrestin-2 and -3 translocation to the cannabinoid CB < sub > 1 < /sub > receptor and the consequences on G protein signalling
This study supports the hypothesis that arrestin-biased ligands for CB1 must engage GRK5/6 rather than GRK2/3, and G protein-biased ligands must have inherently low efficacy.PMID:38604257 | DOI:10.1016/j.bcp.2024.116190 (Source: Biochemical Pharmacology)
Source: Biochemical Pharmacology - April 11, 2024 Category: Drugs & Pharmacology Authors: Jamie J Manning David B Finlay Michelle Glass Source Type: research
The classical D1 dopamine receptor antagonist SCH23390 is a functional sigma-1 receptor allosteric modulator
In this study, we investigated whether SCH23390 functions as an allosteric modulator of Sig1R. We detected increased Sig1R dissociation from binding immunoglobulin protein (BiP) and translocation of Sig1R to the plasma membrane in response to SCH23390 in transfected HEK293T and SH-SY5Y cells, respectively. Activation of Sig1R by SCH23390 was further confirmed by inhibition of GSK3β activity in a time- and dose-dependent manner; this effect was blocked by pretreatment with the Sig1R antagonist, BD1047, and by knockdown of Sig1R. SCH23390 also inhibited GSK3β in wild-type mice but not in Sig1R knockout mice. Finally, we sh...
Source: Acta Pharmacologica Sinica - April 11, 2024 Category: Drugs & Pharmacology Authors: Gu-Fang Zhang Kai-Lian Zhu Qi Li Yue Zhang John L Waddington Xiang-Dong Du Xue-Chu Zhen Source Type: research
Talk to your neighbors in an emergency: Stromule-mediated chloroplast-nucleus communication in plant immunity
Curr Opin Plant Biol. 2024 Apr 10;79:102529. doi: 10.1016/j.pbi.2024.102529. Online ahead of print.ABSTRACTHypersensitive response-programmed cell death (HR-PCD) is a response mounted by plants to defend themselves against pathogens. Communication between the chloroplast and the nucleus is critical for the progression of HR-PCD. Tubular protrusions of chloroplasts, known as stromules, are tightly associated with the HR-PCD progression. There is emerging evidence that signaling molecules originating from chloroplasts are transferred to the nucleus through stromules. The translocation of signaling molecules from the chloropl...
Source: Current Opinion in Plant Biology - April 11, 2024 Category: Biology Authors: Seungmee Jung Jongchan Woo Eunsook Park Source Type: research
Talk to your neighbors in an emergency: Stromule-mediated chloroplast-nucleus communication in plant immunity
Curr Opin Plant Biol. 2024 Apr 10;79:102529. doi: 10.1016/j.pbi.2024.102529. Online ahead of print.ABSTRACTHypersensitive response-programmed cell death (HR-PCD) is a response mounted by plants to defend themselves against pathogens. Communication between the chloroplast and the nucleus is critical for the progression of HR-PCD. Tubular protrusions of chloroplasts, known as stromules, are tightly associated with the HR-PCD progression. There is emerging evidence that signaling molecules originating from chloroplasts are transferred to the nucleus through stromules. The translocation of signaling molecules from the chloropl...
Source: Current Opinion in Plant Biology - April 11, 2024 Category: Biology Authors: Seungmee Jung Jongchan Woo Eunsook Park Source Type: research
Talk to your neighbors in an emergency: Stromule-mediated chloroplast-nucleus communication in plant immunity
Curr Opin Plant Biol. 2024 Apr 10;79:102529. doi: 10.1016/j.pbi.2024.102529. Online ahead of print.ABSTRACTHypersensitive response-programmed cell death (HR-PCD) is a response mounted by plants to defend themselves against pathogens. Communication between the chloroplast and the nucleus is critical for the progression of HR-PCD. Tubular protrusions of chloroplasts, known as stromules, are tightly associated with the HR-PCD progression. There is emerging evidence that signaling molecules originating from chloroplasts are transferred to the nucleus through stromules. The translocation of signaling molecules from the chloropl...
Source: Current Opinion in Plant Biology - April 11, 2024 Category: Biology Authors: Seungmee Jung Jongchan Woo Eunsook Park Source Type: research
Talk to your neighbors in an emergency: Stromule-mediated chloroplast-nucleus communication in plant immunity
Curr Opin Plant Biol. 2024 Apr 10;79:102529. doi: 10.1016/j.pbi.2024.102529. Online ahead of print.ABSTRACTHypersensitive response-programmed cell death (HR-PCD) is a response mounted by plants to defend themselves against pathogens. Communication between the chloroplast and the nucleus is critical for the progression of HR-PCD. Tubular protrusions of chloroplasts, known as stromules, are tightly associated with the HR-PCD progression. There is emerging evidence that signaling molecules originating from chloroplasts are transferred to the nucleus through stromules. The translocation of signaling molecules from the chloropl...
Source: Current Opinion in Plant Biology - April 11, 2024 Category: Biology Authors: Seungmee Jung Jongchan Woo Eunsook Park Source Type: research
A rare complex structural variant of novel intragenic inversion combined with reciprocal translocation t(X;1)(p21.2;p13.3) in Duchenne muscular dystrophy
Duchenne muscular dystrophy (DMD) is the most common form of X-linked recessive muscular dystrophy caused by pathogenic variants in the dystrophin (DMD) gene (OMIM:#310200) [1]. Approximately 1 in 5000 live male births suffer from DMD [2]. The most common dystrophin mutations are deletions and duplications, followed by point mutations [3]. Conventional diagnostic techniques, such as multiplex ligation probe amplification (MLPA) or whole exome sequencing (WES), are typically used to detect pathogenic variants in the DMD gene. (Source: Neuromuscular Disorders)
Source: Neuromuscular Disorders - April 11, 2024 Category: Neurology Authors: Yaye Wang, Xinmei Wen, Xin-Ming Shen, Li Di, Yanan Sun, Yun Li, Shu Zhang, Qi Wen, Jingsi Wang, Jianying Duo, Yue Huang, Yan Lu, Min Xu, Min Wang, Hai Chen, Wenjia Zhu, Yuwei Da Tags: Case report Source Type: research
Investigating the Effects of Perampanel on Autophagy-mediated Regulation of GluA2 and PSD95 in Epilepsy
This study aimed to investigate the intrinsic molecular regulatory mechanisms involved in the downregulation of GluA2, a key subunit of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, following epileptic seizures. Primary mouse hippocampal neurons were cultured and subjected to an epilepsy cell model. The expression levels of GluA2 and autophagy-related proteins were assessed using Western blotting and real-time flu orescent quantitative PCR. Immunofluorescence and immunohistochemistry techniques were employed to investigate the nuclear translocation of CREB-regulated transcriptional coactivator 1 (CRTC1)....
Source: Molecular Neurobiology - April 11, 2024 Category: Neurology Source Type: research
Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis
CONCLUSION: Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.PMID:38596488 | PMC:PMC11000083 | DOI:10.3748/wjg.v30.i10.1405 (Source: World Journal of Gastroenterology : WJG)
Source: World Journal of Gastroenterology : WJG - April 10, 2024 Category: Gastroenterology Authors: Ye Tian Xin Li Xu Wang Si-Ting Pei Hong-Xin Pan Yu-Qi Cheng Yi-Chen Li Wen-Ting Cao Jin-Dong Ding Petersen Ping Zhang Source Type: research
Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis
CONCLUSION: Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.PMID:38596488 | PMC:PMC11000083 | DOI:10.3748/wjg.v30.i10.1405 (Source: World Journal of Gastroenterology)
Source: World Journal of Gastroenterology - April 10, 2024 Category: Gastroenterology Authors: Ye Tian Xin Li Xu Wang Si-Ting Pei Hong-Xin Pan Yu-Qi Cheng Yi-Chen Li Wen-Ting Cao Jin-Dong Ding Petersen Ping Zhang Source Type: research
The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore detection
Chem Commun (Camb). 2024 Apr 10. doi: 10.1039/d4cc00961d. Online ahead of print.ABSTRACTThrough controlling the ssDNA product length of rolling circle amplification with AcyNTP, here we develop a nanopore signal enhancement strategy (STSS), which can successfully transfer the short oligonucleotide targets into long ssDNAs with appropriate lengths that can generate significant translocation currents. By labelling the RCA product with tags such as tetrahedral structures and isothermal amplicons, the resolution, signal specificity, and target range of the STSS can be further extended.PMID:38597243 | DOI:10.1039/d4cc00961d (So...
Source: Chemical Communications - April 10, 2024 Category: Chemistry Authors: Jin Yu Chunxu Yu Yanru Li Chunmiao Yu Yesheng Wang Ruiping Wu Bingling Li Source Type: research
