Rare myxoid pleomorphic liposarcoma: a case report and literature review
We report a case of a middle-aged woman with a rapidly growing abdominal mass that was diagnosed as myxoid pleomorphic liposarcoma, a recently recognised, rare and aggressive subtype of liposarcoma. The tumour exhibits a combination of histological features from both myxoid liposarcoma and pleomorphic liposarcoma. Genetic analysis revealed mutations in TP53 and RB1, along with widespread loss of heterozygosity. However, no DDIT3 gene translocation or MDM2/CDK4 gene amplification was detected. These genetic characteristics can be used to distinguish this type of liposarcoma from others. Two unusual gene fusion/rearrangement...
Source: Journal of Clinical Pathology - April 18, 2024 Category: Pathology Authors: Shen, Y., Zhao, L., Li, A., Peng, Q., Liu, Q., Wang, L., Liu, Z. Tags: Short report Source Type: research
STMND1 is A Phylogenetically Ancient Stathmin which Localizes to Motile Cilia and Exhibits Nuclear Translocation that Is Inhibited When Soluble Tubulin Concentration Increases
Mol Biol Cell. 2024 Apr 17:mbcE23120514. doi: 10.1091/mbc.E23-12-0514. Online ahead of print.ABSTRACTStathmins are small, unstructured proteins that bind tubulin dimers and are implicated in several human diseases, but whose function remains unknown. We characterized a new stathmin, STMND1 (Stathmin Domain Containing 1) as the human representative of an ancient sub-family. STMND1 features a N-terminal myristoylated and palmitoylated motif which directs it to membranes and a tubulin-binding stathmin-like domain (SLD) that contains an internal nuclear localization signal. Biochemistry and proximity labeling showed that STMND...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Xiang Deng Bryan O Seguinot Gary Bradshaw Jong Suk Lee Shannon Coy Marian Kalocsay Sandro Santagata Timothy Mitchison Source Type: research
TLR Engagement Induces an Alternate Pathway for BCR Signaling that Results in PKC δ Phosphorylation
J Immunol. 2024 Apr 17:ji2300784. doi: 10.4049/jimmunol.2300784. Online ahead of print.ABSTRACTRecently, we reported that preexposure of B cells to IL-4 induced an alternate, signalosome-independent BCR signaling pathway leading to protein kinase C (PKC)δ phosphorylation (pTyr311), which occurs in the membrane compartment. This is considered to represent a form of receptor crosstalk and signal integration. Unlike the classical BCR signaling pathway, Lyn kinase is indispensable for BCR-induced downstream events in the alternate pathway. Our previous report that alternate BCR signaling leading to ERK phosphorylation is trig...
Source: Journal of Immunology - April 17, 2024 Category: Allergy & Immunology Authors: Naeem Khan Yongmei Hu Clifford A Lowell Thomas L Rothstein Source Type: research
Characterization of 35 novel NR5A1/SF-1 variants identified in individuals with atypical sexual development: The SF1next study
CONCLUSIONS: Genetic analyses and functional assays do not explain the observed wide phenotype of individuals with these novel NR5A1/SF-1 variants. In nine individuals, additional likely disease-causing variants in other genes were found, strengthening the hypothesis that the broad phenotype of DSD associated with NR5A1/SF-1 variants may be caused by an oligogenic mechanism.PMID:38623954 | DOI:10.1210/clinem/dgae251 (Source: The Journal of Clinical Endocrinology and Metabolism)
Source: The Journal of Clinical Endocrinology and Metabolism - April 16, 2024 Category: Endocrinology Authors: Rawda Naamneh Elzenaty Idoia Martinez de Lapiscina Chrysanthi Kouri Kay-Sara Sauter Grit Sommer Luis Casta ño Christa E Fl ück SF1next study group Source Type: research
Decoding cocaine-induced proteomic adaptations in the mouse nucleus accumbens
Sci Signal. 2024 Apr 16;17(832):eadl4738. doi: 10.1126/scisignal.adl4738. Epub 2024 Apr 16.ABSTRACTCocaine use disorder (CUD) is a chronic neuropsychiatric condition that results from enduring cellular and molecular adaptations. Among substance use disorders, CUD is notable for its rising prevalence and the lack of approved pharmacotherapies. The nucleus accumbens (NAc), a region that is integral to the brain's reward circuitry, plays a crucial role in the initiation and continuation of maladaptive behaviors that are intrinsic to CUD. Leveraging advancements in neuroproteomics, we undertook a proteomic analysis that spanne...
Source: Science Signaling - April 16, 2024 Category: Biomedical Science Authors: Philipp Mews Lucas Sosnick Ashik Gurung Simone Sidoli Eric J Nestler Source Type: research
Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2
This study investigated the impact of FSS and HP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and examined the distinctions and interactions between the two mechanisms. The results demonstrated that both FSS and HP individually enhanced the osteogenic differentiation of BMSCs, with a more pronounced effect observed through their combined application. BMSCs responded to external FSS and HP stimulation through the integrin-cytoskeleton and Piezo1 ion channel respectively. This led to the activation of downstream biochemical signals, resulting in the dephosphorylation and nuclear translocati...
Source: Biotechnology Journal - April 16, 2024 Category: Biotechnology Authors: Yi Zhou Pan Guo Ziyang Jin Miaomiao Chai Shuhong Zhang Xianwei Wang Wen-Song Tan Yan Zhou Source Type: research
GSE252201 New perspective on the origin of adaptive evolution: a study on cytidine deaminase in lampreys
This study evaluates the similarity of adaptive immune mechanisms between jawless and jawed vertebrates using lamprey cytidine deaminase (CDA). We identified the ancestral gene Lr-CDAs of the AID/APOBEC deaminase family and evaluated its biological function in vivo. Lr-CDA1 deletion affected the assembly of three types of variable lymphocyte receptors (VLRs). We identified a switch-like region in lamprey gVLRs bound to Lr-CDAs, which upon repression, downregulated VLRB expression. Overall, we propose that lampreys have an early form of class switch recombination (CSR) that is mediated by Lr-CDAs and acts on gVLRs, affectin...
Source: GEO: Gene Expression Omnibus - April 16, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Lethenteron reissneri Source Type: research
Re-repair after translocation of an anomalous left coronary artery
Ann Thorac Surg. 2024 Apr 13:S0003-4975(24)00282-0. doi: 10.1016/j.athoracsur.2024.03.036. Online ahead of print.ABSTRACTWe repaired a left anomalous aortic origin of coronary artery (AAOCA) using coronary ostium translocation/re-implantation. Re-repair was required due to suboptimal flow pattern in postoperative transesophageal echocardiography (TEE), confirmed with coronary angiography. We emphasize the importance of early recognition, diagnosis and intervention to deal with a problem with the coronary repair.PMID:38621655 | DOI:10.1016/j.athoracsur.2024.03.036 (Source: The Annals of Thoracic Surgery)
Source: The Annals of Thoracic Surgery - April 15, 2024 Category: Cardiovascular & Thoracic Surgery Authors: Alejandro Fernandez-Cisneros Eric Feins Jane Newburger Sepehr Sekhavat Bryan Quinn Luis Quinonez Source Type: research
Elemental composition and potential health risk of vegetable cultivated in residential area situated close to abandoned gold mine dump: Characteristics of soil quality on the vegetables
This study mainly aimed to investigate the elemental distribution of both toxic and essential elements in soils and leafy vegetables (Brassica oleracea) collected from eight different sites around the Davidsonville residential area, located closer to the abandoned Princess gold mine dump, Johannesburg, South Africa. The nutritional value of vegetables in the human diet was determined to assess their value to their health. The vegetables contained metals in the following descending order: Ca > Mg > Ca > Sb > Pb > Fe > Mo > Cr > Se > As > V > Ni > Co > Cd. The bioaccumulation factor (BA...
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - April 15, 2024 Category: Environmental Health Authors: Sihle Mngadi Philiswa Nosizo Nomngongo Shadung Moja Source Type: research
Long-Timescale Atomistic Simulations Uncover Loss-of-Function Mechanisms of Uncharacterized Angiogenin Mutants Associated with ALS
This study focused on investigating two uncharacterized ANG mutations, T11S and R122H, newly identified in the Project Mine consortium. Using extensive computational analysis, including structural modeling and microsecond-timescale molecular dynamics (MD) simulations, we observed conformational changes in the catalytic residue His114 of ANG induced by T11S and R122H mutations. These alterations impaired ribonucleolytic activity, as inferred through molecular docking and binding free energy calculations. Gibbs free energy landscape and residue-residue interaction network analysis further supported our findings, revealing th...
Source: Archives of Biochemistry and Biophysics - April 15, 2024 Category: Biochemistry Authors: Deeksha Dewangan Aryaman Joshi Aditya K Padhi Source Type: research
Re-repair after translocation of an anomalous left coronary artery
Ann Thorac Surg. 2024 Apr 13:S0003-4975(24)00282-0. doi: 10.1016/j.athoracsur.2024.03.036. Online ahead of print.ABSTRACTWe repaired a left anomalous aortic origin of coronary artery (AAOCA) using coronary ostium translocation/re-implantation. Re-repair was required due to suboptimal flow pattern in postoperative transesophageal echocardiography (TEE), confirmed with coronary angiography. We emphasize the importance of early recognition, diagnosis and intervention to deal with a problem with the coronary repair.PMID:38621655 | DOI:10.1016/j.athoracsur.2024.03.036 (Source: The Annals of Thoracic Surgery)
Source: The Annals of Thoracic Surgery - April 15, 2024 Category: Cardiovascular & Thoracic Surgery Authors: Alejandro Fernandez-Cisneros Eric Feins Jane Newburger Sepehr Sekhavat Bryan Quinn Luis Quinonez Source Type: research
Optimizing properties of translocation-enhancing transmembrane proteins
In this study, we demonstrate that specific properties of transmembrane proteins/peptides can enhance membrane permeation of amphiphilic peptides. Using coarse-grained molecular dynamics with free energy calculations, we identify key translocation-enhancing attributes of transmembrane proteins/peptides: a continuous hydrophilic patch, charged residues preferably in the membrane center, and aromatic hydrophobic residues. By employing both coarse-grained and atomistic simulations, complemented by experimental validation, we show that these properties not only enhance peptide translocation but also speed up lipid flip-flop. T...
Source: Biophysical Journal - April 14, 2024 Category: Physics Authors: Ladislav Barto š Martina Drabinov á Robert V ácha Source Type: research
TXNIP deficiency attenuates renal fibrosis by modulating mTORC1/TFEB-mediated autophagy in diabetic kidney disease
In this study, we found that TXNIP knockout suppressed renal fibrosis and activation of mammalian target of rapamycin complex 1 (mTORC1) and restored transcription factor EB (TFEB) and autophagy activation in diabetic kidneys. Simultaneously, TXNIP interference inhibited epithelial-to-mesenchymal transformation (EMT), collagen I and fibronectin expression, and mTORC1 activation, increased TFEB nuclear translocation, and promoted autophagy restoration in HK-2 cells exposed to high glucose (HG). Rapamycin, an inhibitor of mTORC1, increased TFEB nuclear translocation and autophagy in HK-2 cells under HG conditions. Moreover, ...
Source: Renal Failure - April 14, 2024 Category: Urology & Nephrology Authors: Yunxia Du Ming Wu Shan Song Yawei Bian Yonghong Shi Source Type: research
Cerium Oxide and Neodymium Oxide Phytoextraction by Ryegrass in Bioenhanced Hydroponic Environments
In this study, the effect of biostimulation and bioaugmentation on phytorecovery of REE nanoparticles (REE-NP) was investigated by exposing ryegrass seeds to REE-NP in hydroponic environments. This was studied in two sets of experiments: bioaugmentation (using CeO2 nanoparticles and Methylobacterium extorquens AM1 pure culture), and biostimulation (using CeO2 or Nd2O3 nanoparticles and endogenous microorganisms). Addition of M. extorquens AM1 in bioaugmentation experiment including 500 mg/L CeO2 nanoparticles could not promote the nanoparticles accumulation in both natural and surface-sterilized treatments. However, it enh...
Source: Environmental Pollution - April 14, 2024 Category: Environmental Health Authors: Maedeh Soleimanifar Lucia Rodriguez-Freire Source Type: research
Optimizing properties of translocation-enhancing transmembrane proteins
In this study, we demonstrate that specific properties of transmembrane proteins/peptides can enhance membrane permeation of amphiphilic peptides. Using coarse-grained molecular dynamics with free energy calculations, we identify key translocation-enhancing attributes of transmembrane proteins/peptides: a continuous hydrophilic patch, charged residues preferably in the membrane center, and aromatic hydrophobic residues. By employing both coarse-grained and atomistic simulations, complemented by experimental validation, we show that these properties not only enhance peptide translocation but also speed up lipid flip-flop. T...
Source: Biophysical Journal - April 14, 2024 Category: Physics Authors: Ladislav Barto š Martina Drabinov á Robert V ácha Source Type: research
