Molecular mechanism of high glucose-induced mitochondrial DNA damage in retinal ganglion cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):219-224. doi: 10.14715/cmb/2024.70.3.33.ABSTRACTMitochondrial DNA damage in retinal ganglion cells (RGCs) may be closely related to lesions of glaucoma. RGCs were cultured with different concentrations of glucose and grouped into 3 groups, namely normal control (NC) group, Low-Glu group, and High-Glu group. Cell viability was measured with cell counting kit-8, and cell apoptosis was measured using flow cytometry. The DNA damage was measured with comet assay, and the morphological changes of damaged mitochondria in RGCs were observed using TEM. Western blot analyzed the expr...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jinzi Zhou Fenghua Chen Aimin Yan Xiaobo Xia Source Type: research
The mitochondria-targeted antioxidant MitoQ ameliorates inorganic arsenic-induced DCs/Th1/Th2/Th17/Treg differentiation partially by activating PINK1-mediated mitophagy in murine liver
This study has provided new insights on the immunotoxicity of inorganic arsenic and established a foundation for exploring preventive and therapeutic strategies targeting PINK1-mediated mitophagy in acute liver injury. Consequently, the application of mitochondrial antioxidant MitoQ may offer a promising treatment for the metalloid-induced acute liver injury.PMID:38653026 | DOI:10.1016/j.ecoenv.2024.116350 (Source: Ecotoxicology and Environmental Safety)
Source: Ecotoxicology and Environmental Safety - April 23, 2024 Category: Environmental Health Authors: Hui Li Yaning Guo Wei Su Huan Zhang Xiaoxi Wei Xinyu Ma Shuwen Gong Gaoyang Qu Lin Zhang Hong Xu Fuhai Shen Shoufang Jiang Dingjie Xu Jinlong Li Source Type: research
Glaucoma Patient with Suspected Flammer Syndrome: Diagnostic Procedures and Therapeutic Implications
Klin Monbl Augenheilkd. 2024 Apr;241(4):355-360. doi: 10.1055/a-2275-2323. Epub 2024 Apr 23.ABSTRACTIf glaucoma damage develops despite normal intraocular pressure or if the damage progresses despite well-controlled intraocular pressure, we usually find other risk factors. One important group are the vascular factors. We should focus not only on the classical risk factors of atherosclerosis, such as arterial hypertension or dyslipidaemia, but also on dysregulation of blood flow, especially on primary vascular dysregulation (PVD). Low blood pressure, either current or in adolescence, low body mass index or frequently cold h...
Source: Klinische Monatsblatter fur Augenheilkunde - April 23, 2024 Category: Opthalmology Authors: Katarzyna Konieczka Source Type: research
Inhibiting Pink1/Parkin-mediated mitophagy enhances the anticancer effects of quercetin in hepatocellular carcinomaf
In this study, we aimed to explore the interplay among quercetin, mitochondrial dynamics and mitophagy and whether mitophagy-inhibition synergistically enhances the anti-tumor effects of quercetin. Huh7 and Hep3B cells were utilized for in vitro and in vivo studies. Results showed that quercetin treatment significantly increased the expression of mitochondrial fusion genes (MFN1 and MFN2) and decreased the expression of fission genes (DRP1 and FIS1) in Huh7 and Hep3B cells, leading to a more fused and elongated mitochondrial network. Quercetin upregulated the expression of key mitophagy regulators, PINK1 and PARK2, and enh...
Source: Biochemical and Biophysical Research communications - April 23, 2024 Category: Biochemistry Authors: Fang Chen Source Type: research
The role of mitochondrial dynamics and mitophagy in skeletal muscle atrophy: from molecular mechanisms to therapeutic insights
Cell Mol Biol Lett. 2024 Apr 23;29(1):59. doi: 10.1186/s11658-024-00572-y.ABSTRACTSkeletal muscle is the largest metabolic organ of the human body. Maintaining the best quality control and functional integrity of mitochondria is essential for the health of skeletal muscle. However, mitochondrial dysfunction characterized by mitochondrial dynamic imbalance and mitophagy disruption can lead to varying degrees of muscle atrophy, but the underlying mechanism of action is still unclear. Although mitochondrial dynamics and mitophagy are two different mitochondrial quality control mechanisms, a large amount of evidence has indica...
Source: Cellular and Molecular Biology Letters - April 23, 2024 Category: Biochemistry Authors: Yuhang Lei Mailin Gan Yanhao Qiu Qiuyang Chen Xingyu Wang Tianci Liao Mengying Zhao Lei Chen Shunhua Zhang Ye Zhao Lili Niu Yan Wang Li Zhu Linyuan Shen Source Type: research
The Wolfram-like variant WFS1 < sup > E864K < /sup > destabilizes MAM and compromises autophagy and mitophagy in human and mice
Autophagy. 2024 Apr 23:1-12. doi: 10.1080/15548627.2024.2341588. Online ahead of print.ABSTRACTDominant variants in WFS1 (wolframin ER transmembrane glycoprotein), the gene coding for a mitochondria-associated endoplasmic reticulum (ER) membrane (MAM) resident protein, have been associated with Wolfram-like syndrome (WLS). In vitro and in vivo, WFS1 loss results in reduced ER to mitochondria calcium (Ca2+) transfer, mitochondrial dysfunction, and enhanced macroautophagy/autophagy and mitophagy. However, in the WLS pathological context, whether the mutant protein triggers the same cellular processes is unknown. Here, we sho...
Source: Autophagy - April 23, 2024 Category: Cytology Authors: Simone Patergnani M éghane S Bataillard Alberto Danese Stacy Alves Chantal Cazevieille Ren é Valéro Lisbeth Tranebj ærg Tangui Maurice Paolo Pinton Benjamin Delprat Elodie M Richard Source Type: research
Effect of NAMPT on the proliferation and apoptosis in odontoblast-like MDPC-23 cell
This study aimed to evaluate the physiological role of NAMPT associated with MDPC-23 odontoblast cell proliferation. Cell viability was measured using the (DAPI) staining, caspase activation analysis and immunoblotting were performed. Visfatin promoted MDPC-23 odontoblast cell growth in a dose-dependent manner. Furthermore, the up-regulation of Visfatin promoted odontogenic differentiation and accelerated mineralization through an increase in representative odontoblastic biomarkers in MDPC-23 cells. However, FK-866 cell growth in a dose-dependent manner induced nuclear condensation and fragmentation. FK-866-treated cells s...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Kyeong-Rok Kang Joo-Cheol Park Chun Sung Kim Heung-Joong Kim Sun-Kyoung Yu Hong Sung Chun HyangI Lim Jeong-Yeon Seo Jae-Sung Kim Do Kyung Kim Source Type: research
ATF3/EGR1 regulates myocardial ischemia/reperfusion injury induced autophagy and inflammation in cardiomyocytes
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):125-129. doi: 10.14715/cmb/2024.70.3.18.ABSTRACTMyocardial ischemia/reperfusion injury (MIRI) is an irreversible adverse event during the management of coronary heart disease that lacks effective controls. The underlying mechanism of MIRI still requires further investigation. Recent studies have suggested that overexpression of ATF3 protects against MIRI by regulating inflammatory responses, ferroptosis, and autophagy. The downstream target of ATF3, EGR1, also showed cardioprotective properties against MIRI by promoting autophagy. Therefore, further investigating the effect...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Li Li Gang Fu Caiyun Liu Yanling Liu Source Type: research
Molecular mechanism of high glucose-induced mitochondrial DNA damage in retinal ganglion cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):219-224. doi: 10.14715/cmb/2024.70.3.33.ABSTRACTMitochondrial DNA damage in retinal ganglion cells (RGCs) may be closely related to lesions of glaucoma. RGCs were cultured with different concentrations of glucose and grouped into 3 groups, namely normal control (NC) group, Low-Glu group, and High-Glu group. Cell viability was measured with cell counting kit-8, and cell apoptosis was measured using flow cytometry. The DNA damage was measured with comet assay, and the morphological changes of damaged mitochondria in RGCs were observed using TEM. Western blot analyzed the expr...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Jinzi Zhou Fenghua Chen Aimin Yan Xiaobo Xia Source Type: research
Glaucoma Patient with Suspected Flammer Syndrome: Diagnostic Procedures and Therapeutic Implications
Klin Monbl Augenheilkd. 2024 Apr;241(4):355-360. doi: 10.1055/a-2275-2323. Epub 2024 Apr 23.ABSTRACTIf glaucoma damage develops despite normal intraocular pressure or if the damage progresses despite well-controlled intraocular pressure, we usually find other risk factors. One important group are the vascular factors. We should focus not only on the classical risk factors of atherosclerosis, such as arterial hypertension or dyslipidaemia, but also on dysregulation of blood flow, especially on primary vascular dysregulation (PVD). Low blood pressure, either current or in adolescence, low body mass index or frequently cold h...
Source: Klinische Monatsblatter fur Augenheilkunde - April 23, 2024 Category: Opthalmology Authors: Katarzyna Konieczka Source Type: research
Molecules, Vol. 29, Pages 1907: Electropositive Citric Acid-Polyethyleneimine Carbon Dots Carrying the PINK1 Gene Regulate ATP-Related Metabolic Dysfunction in APP/PS1-N2a Cells
Conclusions: These findings indicate that the PINK gene can alleviate AD by increasing bioenergetic metabolism, reducing A&beta;1-40 and A&beta;1-42, and increasing ATP production. (Source: Molecules)
Source: Molecules - April 23, 2024 Category: Chemistry Authors: Si Yu Feng Guo Yuzhen Luo Xingfang Zhang Chenyu Wang Yiheng Liu Haiying Zhang Tags: Article Source Type: research
N-methyl-D-aspartate receptors and thymoquinone induce apoptosis and alteration in mitochondria in colorectal cancer cells
This study aimed to determine the roles of N-Methyl-D-Aspartate agonists and Thymoquinone on mitochondria and apoptosis. HT-29 cells were treated with different TQ and MK-801 concentrations. We analyzed cell viability, apoptosis, and alteration of mitochondria. Cell viability significantly decreased depending on doses of TQ and MK-801. Apoptosis and mitochondrial dysfunctions induced by low and high doses of TQ and MK-801. Our study emphasizes the need for further safety evaluation of MK-801 due to the potential toxicity risk of TQ and MK-801. Optimal and toxic doses of TQ and MK-801 were determined for the treatment of co...
Source: Medical Oncology - April 23, 2024 Category: Cancer & Oncology Source Type: research
Brazilin Actuates Ferroptosis in Breast Cancer Cells via p53/SLC7A11/GPX4 Signaling Pathway
ConclusionsBrazilin actuated ferroptosis in breast cancer cells, and the underlying mechanism is mainly associated with the p53/SLC7A11/GPX4 signaling pathway. (Source: Chinese Journal of Integrative Medicine)
Source: Chinese Journal of Integrative Medicine - April 23, 2024 Category: Internal Medicine Source Type: research
Effect of the Mitochondrial Transaminase (GOT2) on Membrane Potential Sensitive Respiration in Mitochondria of Differentiated C2C12 Muscle Cells
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00576.2023. Online ahead of print.ABSTRACTWe previously showed that the transaminase inhibitor, aminooxyacetic acid, reduced respiration energized at complex II (succinate dehydrogenase, SDH) in mitochondria isolated from mouse hindlimb muscle. The effect required a reduction in membrane potential with resultant accumulation of oxaloacetate (OAA), a potent inhibitor of SDH. To specifically assess the effect of the mitochondrial transaminase, glutamic oxaloacetic transaminase (GOT2) on complex II respiration and to determine the effect in intact cells as well as i...
Source: Am J Physiol Cell Ph... - April 22, 2024 Category: Cytology Authors: Ritu Som Brian D Fink Liping Yu William I Sivitz Source Type: research
Effect of the Mitochondrial Transaminase (GOT2) on Membrane Potential Sensitive Respiration in Mitochondria of Differentiated C2C12 Muscle Cells
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00576.2023. Online ahead of print.ABSTRACTWe previously showed that the transaminase inhibitor, aminooxyacetic acid, reduced respiration energized at complex II (succinate dehydrogenase, SDH) in mitochondria isolated from mouse hindlimb muscle. The effect required a reduction in membrane potential with resultant accumulation of oxaloacetate (OAA), a potent inhibitor of SDH. To specifically assess the effect of the mitochondrial transaminase, glutamic oxaloacetic transaminase (GOT2) on complex II respiration and to determine the effect in intact cells as well as i...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Ritu Som Brian D Fink Liping Yu William I Sivitz Source Type: research
