Lactate Oxidation in Paracoccus denitrificans
We report proteomic, metabolomic, and biochemical studies that establish that the metabolism of lactate by P. denitrificans is mediated by two non-NAD-dependent lactate dehydrogenases. One prefers D-lactate over L-lactate (D-iLDH) and the other prefers L-lactate (L-iLDH). We cloned and produced the D-iLDH and characterized it. The Km for D-lactate was 34 μM, and for L-lactate it was 3.7 mM. Pyruvate was not a substrate, rendering the reaction unidirectional with lactate being converted to pyruvate for entry into the TCA cycle. The intracellular lactate was ∼14 mM such that both isomers could be metabolized by the enzyme...
Source: Archives of Biochemistry and Biophysics - April 17, 2024 Category: Biochemistry Authors: Geumsoo Kim Raul Covian Lanelle Edwards Yi He Robert S Balaban Rodney L Levine Source Type: research
New insights into the structure and dynamics of the TOM complex in mitochondria
Biochem Soc Trans. 2024 Apr 17:BST20231236. doi: 10.1042/BST20231236. Online ahead of print.ABSTRACTTo date, there is no general physical model of the mechanism by which unfolded polypeptide chains with different properties are imported into the mitochondria. At the molecular level, it is still unclear how transit polypeptides approach, are captured by the protein translocation machinery in the outer mitochondrial membrane, and how they subsequently cross the entropic barrier of a protein translocation pore to enter the intermembrane space. This deficiency has been due to the lack of detailed structural and dynamic informa...
Source: Biochemical Society Transactions - April 17, 2024 Category: Biochemistry Authors: Stephan Nussberger Robin Ghosh Shuo Wang Source Type: research
Modulation of mitochondrial function by extracellular acidosis in tumor cells and normal fibroblasts: Role of signaling pathways
Neoplasia. 2024 Apr 16;52:100999. doi: 10.1016/j.neo.2024.100999. Online ahead of print.ABSTRACTIn many tumors pronounced extracellular acidosis resulting from glycolytic metabolism is found. Since several environmental stress factors affect the mitochondrial activity the aim of the study was to analyze the impact of acidosis on cellular oxygen consumption and which signaling pathways may be involved in the regulation. In two tumor cell lines and normal fibroblasts cellular oxygen consumption rate (OCR) and mitochondrial function were measured after 3 h at pH 6.6. Besides the activation of ERK1/2, p38 and PI3K signaling in...
Source: Neoplasia - April 17, 2024 Category: Cancer & Oncology Authors: Carmen Degitz Sarah Reime Christina-Marie Baumbach Mandy Rauschner Oliver Thews Source Type: research
Electroacupuncture Inhibits Neural Ferroptosis in Rat Model of Traumatic Brain Injury via Activating System Xc-/GSH/GPX4 Axis
CONCLUSIONS: EA inhibits neuronal ferroptosis by up-regulated xCT expression and by activating System Xc-/GSH/GPX4 axis after TBI, confirming the relevant theories regarding the EA effect in treating TBI and providing theoretical support for clinical practice.PMID:38629369 | DOI:10.2174/0115672026297775240405073502 (Source: Current Neurovascular Research)
Source: Current Neurovascular Research - April 17, 2024 Category: Neurology Authors: Na Li Ruihui Wang Xia Ai Jie Guo Yuwang Bai Xinrong Guo Rongchao Zhang Xu Du Jingxuan Chen Hua Li Source Type: research
New insights into the structure and dynamics of the TOM complex in mitochondria
Biochem Soc Trans. 2024 Apr 24;52(2):911-922. doi: 10.1042/BST20231236.ABSTRACTTo date, there is no general physical model of the mechanism by which unfolded polypeptide chains with different properties are imported into the mitochondria. At the molecular level, it is still unclear how transit polypeptides approach, are captured by the protein translocation machinery in the outer mitochondrial membrane, and how they subsequently cross the entropic barrier of a protein translocation pore to enter the intermembrane space. This deficiency has been due to the lack of detailed structural and dynamic information about the membra...
Source: Biochemical Society Transactions - April 17, 2024 Category: Biochemistry Authors: Stephan Nussberger Robin Ghosh Shuo Wang Source Type: research
Modulation of mitochondrial function by extracellular acidosis in tumor cells and normal fibroblasts: Role of signaling pathways
Neoplasia. 2024 Apr 16;52:100999. doi: 10.1016/j.neo.2024.100999. Online ahead of print.ABSTRACTIn many tumors pronounced extracellular acidosis resulting from glycolytic metabolism is found. Since several environmental stress factors affect the mitochondrial activity the aim of the study was to analyze the impact of acidosis on cellular oxygen consumption and which signaling pathways may be involved in the regulation. In two tumor cell lines and normal fibroblasts cellular oxygen consumption rate (OCR) and mitochondrial function were measured after 3 h at pH 6.6. Besides the activation of ERK1/2, p38 and PI3K signaling in...
Source: Neoplasia - April 17, 2024 Category: Cancer & Oncology Authors: Carmen Degitz Sarah Reime Christina-Marie Baumbach Mandy Rauschner Oliver Thews Source Type: research
Electroacupuncture Inhibits Neural Ferroptosis in Rat Model of Traumatic Brain Injury via Activating System Xc-/GSH/GPX4 Axis
CONCLUSIONS: EA inhibits neuronal ferroptosis by up-regulated xCT expression and by activating System Xc-/GSH/GPX4 axis after TBI, confirming the relevant theories regarding the EA effect in treating TBI and providing theoretical support for clinical practice.PMID:38629369 | DOI:10.2174/0115672026297775240405073502 (Source: Current Neurovascular Research)
Source: Current Neurovascular Research - April 17, 2024 Category: Neurology Authors: Na Li Ruihui Wang Xia Ai Jie Guo Yuwang Bai Xinrong Guo Rongchao Zhang Xu Du Jingxuan Chen Hua Li Source Type: research
Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway
In conclusion, our study reveals for the first time that nerolidol can ameliorate hippocampal damage, neuroinflammation, synaptic, ER, and mitochondrial damage in diabetic rats. Furthermore, we suggest that nerolidol may inhibit NLRP3 inflammasome activation via MAPK and AKT signaling pathways. These findings provide a new experimental basis for the use of nerolidol to ameliorate diabetes-induced brain tissue damage and the associated disease. AbstractDespite the observation of diabetes-induced brain tissue damage and impaired learning and memory, the underlying mechanism of damage remains elusive, and effective, targeted ...
Source: BioFactors - April 17, 2024 Category: Biochemistry Authors: Yining Lei,
Manqin Li,
Xinran Liu,
Lu Zhang,
Ruyi Zhang,
Fei Cai Tags: RESEARCH ARTICLE Source Type: research
The effects of photobiomodulation on the improvement of sperm parameters: A review study
In this study, a comprehensive analysis of the existing literature, specifically examining the mechanisms of action of PBM has been presented. Our objective was to enhance knowledge in the field of laser light therapy in order to promote the usage of irradiation in clinical settings in a more effective way. Within the realm of reproductive science, the utilization of PBM has been employed to enhance the metabolic processes, motility, and viability of spermatozoa. This is attributed to its advantageous effects on mitochondria, resulting in the activation of the mitochondrial respiratory chain and subsequent synthesis of ATP...
Source: Photochemistry and Photobiology - April 17, 2024 Category: Science Authors: Ali Parvin,
Gisou Erabi,
Mohammad Reza Saboohi Tasooji,
Sonia Sadeghpour,
Hassan Mellatyar,
Sahar Rezaei Arablouydareh,
Leila Navapour,
Mortaza Taheri‐Anganeh,
Hojat Ghasemnejad‐Berenji Tags: INVITED REVIEW Source Type: research
Spatial and single-cell explorations uncover prognostic significance and immunological functions of mitochondrial calcium uniporter in breast cancer
The mitochondrial calcium uniporter (MCU) is a transmembrane protein facilitating the entry of calcium ions into mitochondria from the cell cytosol. Maintaining calcium balance is crucial for enhancing cellula... (Source: Cancer Cell International)
Source: Cancer Cell International - April 17, 2024 Category: Cancer & Oncology Authors: Chia-Jung Li, Yen-Dun Tony Tzeng, Jui-Hu Hsiao, Ling-Ming Tseng, Tzu-Sheng Hsu and Pei-Yi Chu Tags: Research Source Type: research
Attenuating mitochondrial dysfunction and morphological disruption with PT320 delays dopamine degeneration in MitoPark mice
Mitochondria are essential organelles involved in cellular energy production. Changes in mitochondrial function can lead to dysfunction and cell death in aging and age-related disorders. Recent research sugges... (Source: Journal of Biomedical Science)
Source: Journal of Biomedical Science - April 17, 2024 Category: Biomedical Science Authors: Vicki Wang, Kuan-Yin Tseng, Tung-Tai Kuo, Eagle Yi-Kung Huang, Kuo-Lun Lan, Zi-Rong Chen, Kuo-Hsing Ma, Nigel H. Greig, Jin Jung, Ho-II Choi, Lars Olson, Barry J. Hoffer and Yuan-Hao Chen Tags: Research Source Type: research
Focusing on mitochondria in the brain: from biology to therapeutics
AbstractMitochondria have multiple functions such as supplying energy, regulating the redox status, and producing proteins encoded by an independent genome. They are closely related to the physiology and pathology of many organs and tissues, among which the brain is particularly prominent. The brain demands 20% of the resting metabolic rate and holds highly active mitochondrial activities. Considerable research shows that mitochondria are closely related to brain function, while mitochondrial defects induce or exacerbate pathology in the brain. In this review, we provide comprehensive research advances of mitochondrial bio...
Source: Translational Neurodegeneration - April 17, 2024 Category: Neurology Source Type: research
Autophagy-dependent lysosomal calcium overload and the ATP5B-regulated lysosomes-mitochondria calcium transmission induce liver insulin resistance under perfluorooctane sulfonate exposure
In this study, we found that PFOS exposure noticeably raised lysosomal calcium in L-02 hepatocytes from 0.5 h. In the PFOS-cultured L-02 cells, inhibiting autophagy alleviated lysosomal calcium overload. Inhibition of mitochondrial calcium uptake aggravated the accumulation of lysosomal calcium, while inhibition of lysosomal calcium outflowing reversed PFOS-induced mitochondrial calcium overload and IR. Transient receptor potential mucolipin 1 (TRPML1), the calcium output channel of lysosomes, interacted with voltage-dependent anion channel 1 (VDAC1), the calcium intake channel of mitochondria, in the PFOS-cultured cells. ...
Source: Ecotoxicology and Environmental Safety - April 16, 2024 Category: Environmental Health Authors: Jixun Li Yu Ma Tianming Qiu Jianyu Wang Jingyuan Zhang Xiance Sun Liping Jiang Qiujuan Li Xiaofeng Yao Source Type: research
Neuronal control of microglia through the mitochondria
This study provides evidence that neurons may trigger microglia activation and subsequent neuroinflammation. In addition, we demonstrate that microglial activation may have a protective role in ameliorating neuronal innate immune activation, at least in the initial phase. This work challenges the current understanding of neuroinflammation by assigning the primary role to neurons.PMID:38626829 | DOI:10.1016/j.bbadis.2024.167167 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: A R Pereira-Santos Emanuel Candeias J D Magalh ães Nuno Empadinhas A Raquel Esteves Sandra M Cardoso Source Type: research
Exercise-induced cardiac mitochondrial reorganization and enhancement in spontaneously hypertensive rats
Pflugers Arch. 2024 Apr 16. doi: 10.1007/s00424-024-02956-7. Online ahead of print.ABSTRACTThe myocardium is a highly oxidative tissue in which mitochondria are essential to supply the energy required to maintain pump function. When pathological hypertrophy develops, energy consumption augments and jeopardizes mitochondrial capacity. We explored the cardiac consequences of chronic swimming training, focusing on the mitochondrial network, in spontaneously hypertensive rats (SHR). Male adult SHR were randomized to sedentary or trained (T: 8-week swimming protocol). Blood pressure and echocardiograms were recorded, and hearts...
Source: Pflugers Archiv : European Journal of Physiology - April 16, 2024 Category: Physiology Authors: Joshua Godoy Coto Erica V Pereyra Fiorella A Cavalli Carlos A Valverde Claudia I Caldiz Sabina M Mat é Alejandra M Yeves Irene L Ennis Source Type: research
