Based on RNA-Seq analysis identification and expression analysis of Trans-scripusinA synthesize-related genes of UV-treatment in postharvest grape fruit.
Abstract Stilbenes, an active substances closely related to resistance and quality of grapes, are rarely found in natural resources. However its cumulative amount is affected by ultraviolet radiation (UV). The purpose of this study is to screen key genes in biosynthesis of stilbenes Trans-scripusin A and explore its synthetic pathway. We tested content of stilbenes with UHPLC-QQQ-MS2, results revealed that stilbenes accumulation is positively correlated with UV-B exposure time. Then, we performed transcriptome high-throughput sequencing of grapes under treatments. Results shown that 13,906 differentially expressed...
Source: Archives of Biochemistry and Biophysics - July 2, 2020 Category: Biochemistry Authors: Kong Q, Deng R, Li X, Zeng Q, Zhang X, Yu X, Ren X Tags: Arch Biochem Biophys Source Type: research

Expression of ectopic trypsin in atherosclerotic plaques and the effects of aprotinin on plaque stability.
CONCLUSIONS: Ectopic trypsin was significantly upregulated in atherosclerotic plaques, which increased pro-inflammatory cytokine levels by activating PAR-2 and promoted plaque instability by activating proMMP-9, thereby promoting atherosclerosis and plaque vulnerability. In addition, the high trypsin expression in human coronary intimal atherosclerotic plaques suggests that targeting trypsin may be a new strategy for acute coronary syndrome prevention. PMID: 32603715 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - June 27, 2020 Category: Biochemistry Authors: Shi JY, Pan HY, Liu K, Pan M, Si GJ Tags: Arch Biochem Biophys Source Type: research

Principal component analysis of data from NMR titration experiment of uniformly 15N labeled amyloid beta (1-42) peptide with osmolytes and phenolic compounds.
Abstract A simple NMR method to analyze the data obtained by NMR titration experiment of amyloid formation inhibitors against uniformly 15N-labeled amyloid-β 1-42 peptide (Aβ(1-42)) was described. By using solution nuclear magnetic resonance (NMR) measurement, the simplest method for monitoring the effects of Aβ fibrilization inhibitors is the NMR chemical shift perturbation (CSP) experiment using 15N-labeled Aβ(1-42). However, the flexible and dynamic nature of Aβ(1-42) monomer may hamper the interpretation of CSP data. Here we introduced principal component analysis (PCA) for visualizing...
Source: Archives of Biochemistry and Biophysics - June 25, 2020 Category: Biochemistry Authors: Iwaya N, Goda N, Matsuzaki M, Narita A, Shigemitsu Y, Tenno T, Abe Y, Hoshi M, Hiroaki H Tags: Arch Biochem Biophys Source Type: research

Conformational dynamics of p53  N-terminal TAD2 region under different solvent conditions.
This study is highlighting the importance of hydrophobic surfaces playing a crucial role in TAD2 interaction and conformational transitions. PMID: 32592801 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - June 24, 2020 Category: Biochemistry Authors: Kumar D, Mishra PM, Gadhave K, Giri R Tags: Arch Biochem Biophys Source Type: research

Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor exerts greater efficacy than atorvastatin on improvement of brain function and cognition in obese rats.
Abstract The accumulation of lipid as a result of long-term consumption of a high-fat diet (HFD) may lead to metabolic and brain dysfunction. Atorvastatin, a recommended first-line lipid-lowering agent, has shown beneficial effects on metabolic and brain functions in several models. Recently, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor was approved as an effective therapeutic drug for dyslipidemia patients. However, few studies have reported on the effect of this PCSK9 inhibitor on brain function. In addition, the comparative efficacy on the improvement of metabolic and brain functions between ...
Source: Archives of Biochemistry and Biophysics - June 24, 2020 Category: Biochemistry Authors: Arunsak B, Pratchayasakul W, Amput P, Chattipakorn K, Tosukhowong T, Kerdphoo S, Jaiwongkum T, Thonusin C, Palee S, Chattipakorn N, Chattipakorn S Tags: Arch Biochem Biophys Source Type: research

Biomarkers of placental redox imbalance in pregnancies with preeclampsia and consequent perinatal outcomes.
a ACM Abstract OBJECTIVE: To compare redox and inflammatory markers between normal and PE-derived placentas and to evaluate the relationship between placental redox imbalance markers and perinatal outcomes in pregnancies with PE. METHODS: This was a cross-sectional study conducted at the maternity hospital of a university hospital in Maceio-Alagoas, Brazil, in 2017, including women diagnosed with PE and healthy pregnant women and their conceptuses. After screening, standardized questionnaires containing socioeconomic, clinical, obstetric and anthropometric data were applied. After delivery, placental samples ...
Source: Archives of Biochemistry and Biophysics - June 24, 2020 Category: Biochemistry Authors: Ferreira RC, Fragoso MBT, Tenório MCDS, Martins ASDP, Borbely AU, Moura FA, Goulart MOF, Oliveira ACM Tags: Arch Biochem Biophys Source Type: research

A 3'-tRNA-derived fragment enhances cell proliferation, migration and invasion in gastric cancer by targeting FBXO47.
Abstract Increasing evidence demonstrates that tRNA-derived fragments (tRFs) exert important effects and are dysregulated in various human cancer types. However, their roles in gastric cancer (GC) remain unknown. Here we identified the functional effects of tRF-3019a (derived from tRNA-Ala-AGC-1-1) in GC. We demonstrated that tRF-3019a was upregulated in GC tissues and cell lines. Phenotypic studies revealed that tRF-3019a overexpression enhances GC cell proliferation, migration and invasion. Conversely, tRF-3019a knockdown inhibits GC cell malignant activities. Mechanistic investigation implies that tRF-3019a dir...
Source: Archives of Biochemistry and Biophysics - June 24, 2020 Category: Biochemistry Authors: Zhang F, Shi J, Wu Z, Gao P, Zhang W, Qu B, Wang X, Song Y, Wang Z Tags: Arch Biochem Biophys Source Type: research

Characterization of oxyphytosterols generated by β-sitosterol ozonization.
Characterization of oxyphytosterols generated by β-sitosterol ozonization. Arch Biochem Biophys. 2020 Jun 23;:108472 Authors: Martins IR, Onuki J, Miyamoto S, Uemi M Abstract β-Sitosterol (βSito) is the most abundant phytosterol found in elevated concentrations in vegetable oils, nuts, seeds, cereals, fruits, and in many phytosterol-enriched foods. Although the benefits, there is a concern in terms of food quality and health due to the increasing consumption of phytosterols and the possible adverse side effects of their oxidation products, oxyphytosterols. βSito has a similar struc...
Source: Archives of Biochemistry and Biophysics - June 23, 2020 Category: Biochemistry Authors: Martins IR, Onuki J, Miyamoto S, Uemi M Tags: Arch Biochem Biophys Source Type: research

The impact of altered mechanobiology on aortic valve pathophysiology.
Abstract Calcific aortic valve disease (CAVD) is the most prevalent valvulopathy worldwide. Until recently, CAVD was viewed as a passive, degenerative process and an inevitable consequence of aging. Recent improvements in disease modeling, imaging, and analysis have greatly enhanced our understanding of CAVD. The aortic valve and its constituent cells are subjected to extreme changes in mechanical forces, so it follows that any changes in the underlying mechanobiology of the valve and its cells would have dire effects on function. Further, the mechanobiology of the aortic valve is intimately intertwined with numer...
Source: Archives of Biochemistry and Biophysics - June 23, 2020 Category: Biochemistry Authors: Bardon KM, Garelnabi M Tags: Arch Biochem Biophys Source Type: research

Dietary walnut as food factor to rescue from NSAID-induced gastrointestinal mucosal damages.
Abstract Nuclear factor erythroid-derived 2-like 2 (Nrf-2) is transcription factor implicated in the antioxidant response element-mediated induction of endogenous antioxidant enzyme such as heme oxygenase-1 (HO-1), glutamate-cysteine ligase, and NAD(P)H quinone dehydrogenase 1, among which HO-1 is an enzyme catalyzing the degradation of heme.producing biliverdin, ferrous iron, and carbon monoxide. In the stomach, as much as regulating gastric acid secretions, well-coordinated establishment of defense system stands for maintaining gastric integrity. In previous study, author et al. for the first time discovered HO-...
Source: Archives of Biochemistry and Biophysics - June 23, 2020 Category: Biochemistry Authors: An JM, Kim EH, Lee H, Lee HJ, Hahm KB Tags: Arch Biochem Biophys Source Type: research

Cold atmospheric plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in glioblastoma multiforme cells.
Abstract Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5 mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) ...
Source: Archives of Biochemistry and Biophysics - June 23, 2020 Category: Biochemistry Authors: Manaloto E, Gowen A, Lesniak A, He Z, Casey A, Cullen PJ, Curtin JF Tags: Arch Biochem Biophys Source Type: research

LINC00261 inhibits progression of pancreatic cancer by down-regulating miR-23a-3p.
In conclusion, LINC00261 overexpression repressed cell viability and invasion and enhanced apoptosis by decreasing miR-23a-3p expression in pancreatic cancer cells, indicating a new target for the treatment of pancreatic cancer. PMID: 32590069 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - June 23, 2020 Category: Biochemistry Authors: Wang X, Gao X, Tian J, Zhang R, Qiao Y, Hua X, Shi G Tags: Arch Biochem Biophys Source Type: research

Proteome-wide assessment of diabetes mellitus in Qatari identifies IGFBP-2 as a risk factor already with early glycaemic disturbances.
Abstract BACKGROUND: Proteomics is expected to provide novel insights in the underlying pathophysiology of type 2 diabetes mellitus. In the present study, we aimed to identify and biochemically characterize proteins associated with diabetes mellitus in a Qatari population. METHODS: In a diabetes case-control study (175 cases, 164 controls; Arab, South Asian and Philippine ethnicities), we conducted a discovery study to screen 1,141 blood protein levels for associations with diabetes mellitus. Additional analyses were done in controls in relation to Hb1Ac, and biochemical characterization of the main findings ...
Source: Archives of Biochemistry and Biophysics - June 22, 2020 Category: Biochemistry Authors: Noordam R, van Heemst D, Suhre K, Krumsiek J, Mook-Kanamori DO Tags: Arch Biochem Biophys Source Type: research

Triosephosphate isomerase deficiency: Effect of F240L mutation on enzyme structure.
Abstract Eleven missense mutations have been describe in human triosephosphate isomerase (TPI), affecting its catalytic function. Several of these mutations generate triosephosphate isomerase deficiency, the consequences of which can in some cases be lethal. The missense F240L mutation was found in a Hungarian patient showing symptoms of chronic hemolytic anemia and neuromuscular dysfunction. In vitro studies using a recombinant version of this mutant showed that it affects kinetic parameters, thermal stability and dimeric stability. Using X-ray crystal structures, the present paper describes how this mutation aff...
Source: Archives of Biochemistry and Biophysics - June 22, 2020 Category: Biochemistry Authors: Romero JM Tags: Arch Biochem Biophys Source Type: research

High-yield expression of extracellular lipase from Yarrowia lipolytica and its interactions with lipopeptide biosurfactants: A biophysical approach.
Abstract The unconventional yeast Yarrowia lipolytica is known as a producer of extracellular lipases. Here we overexpressed extracellular lipase (YlLip2) in yeast strain Y. lipolytica AJD ΔXΔA-Lip2 harboring the overexpression cassette of the YALI0A20350 gene under the strong hybrid promoter UAS1B16-TEF. To maintain a high level of expression of YlLip2 two extracellular proteases of Y. lipolytica, AEPp and AXPp, were deleted. The purified recombinant YlLip2 presented optimal catalytic activities at 37 °C and pH 8.0. The effect of two lipopeptide biosurfactants, i.e., amphisin produced by Pseudom...
Source: Archives of Biochemistry and Biophysics - June 22, 2020 Category: Biochemistry Authors: Janek T, Mirończuk AM, Rymowicz W, Dobrowolski A Tags: Arch Biochem Biophys Source Type: research

Sirt3 promotes hepatocellular carcinoma cells sensitivity to regorafenib through the acceleration of mitochondrial dysfunction.
This study aimed to investigate the mechanism of Sirt3 involved in the mitochondrial dysfunction which associated with regorafenib treatment in liver cancer cells. We found regorafenib inhibited Sirt3 and p-ERK expression in HCC cells in a dose-dependent manner. Bioinformatics analysis showed that Sirt3 expression was down-regulated in liver cancer tissues and its low expression was correlated with worse overall survival (OS) in liver cancer patients. After transfected with Sirt3 overexpression plasmid, we found that Sirt3 sensitized liver cancer cells to regorafenib and resulted in much more apoptosis with a significant i...
Source: Archives of Biochemistry and Biophysics - June 17, 2020 Category: Biochemistry Authors: Wang R, Liu Y, Mi X, Chen Q, Jiang P, Hou J, Lin Y, Li S, Ji B, Fang Y Tags: Arch Biochem Biophys Source Type: research

DNA demethylation increases NETosis.
This study establishes a relationship between NETosis and epigenetics for the first time, and indicates that various diseases implicated to have an epigenetic component might be exacerbated by excessive NETosis also under epigenetic control. PMID: 32561201 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - June 16, 2020 Category: Biochemistry Authors: Yasuda H, Takishita Y, Morita A, Tsutsumi T, Tsuchiya M, Sato EF Tags: Arch Biochem Biophys Source Type: research

The metal- and substrate-dependences of 2,4'-Dihydroxyacetophenone dioxygenase.
Abstract While the enzyme, 2,4'-dihydroxyacetophenone dioxygenase (DAD), has been known for decades, very little has been characterized of the mechanism of the DAD-catalyzed oxidative cleavage of its reported substrate, 2,4'-dihydroxyacetophenone (DHA). The purpose of this study was to identify the active metal center and to characterize the substrate-dependence of the kinetics of the reaction to lay the foundation for deeper mechanistic investigation. To this, the DAD V1M mutant (bDAD) was overexpressed, purified, and reconstituted with various metal ions. Kinetic assays evaluating the activity of the reconstitut...
Source: Archives of Biochemistry and Biophysics - June 9, 2020 Category: Biochemistry Authors: Roberts KM, Connor GC, Cave CH, Rowe GT, Page CA Tags: Arch Biochem Biophys Source Type: research

Resveratrol potentiates the anti-tumor effects of rapamycin in papillary thyroid cancer: PI3K/AKT/mTOR pathway involved.
Abstract The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway plays an important role in the development of papillary thyroid cancer. While rapamycin has been shown to exhibit anti-tumor effects, it may also activate AKT, resulting in increased cell survival and drug resistance, thereby limiting its anti-tumor effects. Resveratrol can also inhibit tumor growth by regulating the PI3K/AKT/mTOR signaling pathway. The present study investigated the anti-tumor effects of the combined use of rapamycin and resveratrol in papillary thyroid cancer. We first ...
Source: Archives of Biochemistry and Biophysics - June 9, 2020 Category: Biochemistry Authors: Bian P, Hu W, Liu C, Li L Tags: Arch Biochem Biophys Source Type: research

Alpha-neoendorphin can reduce UVB-induced skin photoaging by activating cellular autophagy.
Abstract Skin aging is influenced by several genetic, physiological, and environmental factors. In particular, ultraviolet (UV) exposure is an important factor involved in inducing skin photoaging. Autophagy controlling homeostatic balance between the synthesis, degradation, and recycling of cellular organelles and proteins plays important regulatory roles in several biological processes, including aging. The opioid neuropeptide α-neoendorphin (named NEP) is an endogenous decapeptide (N-YGGFLRKYPK-C) that activates the kappa opioid receptor and exhibits certain anti-aging and anti-wrinkling effects on skin c...
Source: Archives of Biochemistry and Biophysics - June 8, 2020 Category: Biochemistry Authors: Lim GE, Park JE, Cho YH, Lim DS, Kim AJ, Moh SH, Lee JH, Lee JS Tags: Arch Biochem Biophys Source Type: research

Dietary nitrate attenuated endothelial dysfunction and atherosclerosis in apolipoprotein E knockout mice fed a high-fat diet: A critical role for NADPH oxidase.
Abstract Nitric oxide (NO) deficiency and NADPH oxidase plays key roles in endothelial dysfunction and atherosclerotic plaque formation. Recent evidence demonstrates that nitrate-nitrite-NO pathway in vivo exerts beneficial effects upon the cardiovascular system. We aimed to investigate the effects of dietary nitrate on endothelial function and atherosclerosis in apolipoprotein E knockout (ApoE-/-) mice fed a high-fat diet. It was shown that dietary nitrate significantly attenuated aortic endothelial dysfunction and atherosclerosis in ApoE-/- mice. Mechanistic studies revealed that dietary nitrate significantly im...
Source: Archives of Biochemistry and Biophysics - June 7, 2020 Category: Biochemistry Authors: Peng R, Luo M, Tian R, Lu N Tags: Arch Biochem Biophys Source Type: research

Estrogen receptors participate in silibinin-caused nuclear translocation of apoptosis-inducing factor in human breast cancer MCF-7 cells.
Abstract Our previous studies showed that silibinin promoted activation of caspases to induce apoptosis in human breast cancer MCF-7 cells by down-regulating the protein expression level of estrogen receptor (ER) α and up-regulating ERβ. Recently, it has been reported that silibinin-induced apoptosis also involved nuclear translocation of apoptosis-inducing factor (AIF). Here we report that silibinin induces nuclear translocation of AIF through the down-regulation of ERα and up-regulation of ERβ in a concentration dependent manner in MCF-7 cells. AIF knockdown with siRNA significantly rev...
Source: Archives of Biochemistry and Biophysics - June 7, 2020 Category: Biochemistry Authors: Liu W, Ji Y, Sun Y, Si L, Fu J, Hayashi T, Onodera S, Ikejima T Tags: Arch Biochem Biophys Source Type: research

Liver fibrogenesis: UN update on established and emerging basic concepts.
Abstract Liver fibrogenesis is defined as a dynamic and highly integrated process occurring during chronic injury to liver parenchyma that can result in excess deposition of extracellular matrix (ECM) components (i.e., liver fibrosis). Liver fibrogenesis, together with chronic inflammatory response, is then primarily involved in the progression of chronic liver diseases (CLD) irrespective of the specific etiology. In the present review we will first offer a synthetic and updated overview of major basic concepts in relation to the role of myofibroblasts (MFs), macrophages and other hepatic cell populations involved...
Source: Archives of Biochemistry and Biophysics - June 7, 2020 Category: Biochemistry Authors: Novo E, Bocca C, Foglia B, Protopapa F, Maggiora M, Parola M, Cannito S Tags: Arch Biochem Biophys Source Type: research

Role of lycopene in smoke-promoted chronic obstructive pulmonary disease and lung carcinogenesis.
Abstract Chronic obstructive pulmonary disease (COPD) and lung cancer are a major cause of morbidity and mortality worldwide, with cigarette smoking being the single most important risk factor for both. Emerging evidence indicate alterations in reverse cholesterol transport-mediated removal of excess cholesterol from lung, and intracellular cholesterol overload to be involved in smoke-promoted COPD and lung cancer development. Since there are currently few effective treatments for COPD and lung cancer, it is important to identify food-derived, biologically active compounds, which can protect against COPD and lung ...
Source: Archives of Biochemistry and Biophysics - June 3, 2020 Category: Biochemistry Authors: Rakic JM, Wang XD Tags: Arch Biochem Biophys Source Type: research

Selenoprotein I is essential for murine embryogenesis.
Abstract Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI-/+ mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ∼ E6, blastocyst embryos (E3.5-E4.4) wer...
Source: Archives of Biochemistry and Biophysics - June 2, 2020 Category: Biochemistry Authors: Avery J, Yamazaki Y, Hoffmann FW, Folgelgren B, Hoffmann PR Tags: Arch Biochem Biophys Source Type: research

Mechanisms involved in the unbalanced redox homeostasis in osteoblastic cellular model of Alkaptonuria.
Abstract Alkaptonuria (AKU) is a rare metabolic disease correlated with the deficiency of homogentisate 1,2-dioxygenase and leading to an accumulation of the metabolite homogentisic acid (HGA) which can be subjected to oxidation and polymerization reactions. These events are considered a trigger for the induction of oxidative stress in AKU but, despite the large description of an altered redox status, the underlying pathogenetic processes are still unstudied. In the present study, we investigated the molecular mechanisms responsible for the oxidative damage present in an osteoblast-based cellular model of AKU. Bon...
Source: Archives of Biochemistry and Biophysics - June 2, 2020 Category: Biochemistry Authors: Schiavone ML, Pecorelli A, Woodby B, Ferrara F, Pambianchi E, Santucci A, Valacchi G Tags: Arch Biochem Biophys Source Type: research

Role of amino acids in regulation of ROS balance in cancer.
Abstract Cancer cells display increased oxidative stress from reactive oxygen species (ROS) and constantly have to counteract them below a tolerable threshold to avoid any toxicity due to overload of ROS. The involvement of ROS in cancer progression from precursor lesions to aggressive tumor and metastasis formation is still debated, but it is recognized that cancer cells succeed to use ROS for their own benefit in circumstances that are tumor cell-type specific. In this review we focus on amino acids' metabolic pathways that tumor cells activate as antioxidants including cysteine, methionine metabolisms and their...
Source: Archives of Biochemistry and Biophysics - June 1, 2020 Category: Biochemistry Authors: Jaune-Pons E, Vasseur S Tags: Arch Biochem Biophys Source Type: research

HCN2 activation modulation: An electrophysiological and molecular study of the well-preserved LCI sequence in the pore channel.
In this study we used site-directed mutagenesis and electrophysiology in frog oocytes to determine whether the LCI sequence affects the kinetics of HCN2 currents. Permeability and voltage dependence were evaluated, and we found a role of LCI in the gating mechanism combined with changes in ion permeability. The I residue resulted critical to this function. PMID: 32492375 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 31, 2020 Category: Biochemistry Authors: Hernandez A, Hernández-Centeno R, Espino-Saldaña ÁE, Martínez-Torres A Tags: Arch Biochem Biophys Source Type: research

The leucine-rich repeat domain of human peroxidasin 1 promotes binding to laminin in basement membranes.
In this study, we probed the interactions of four PXDN constructs with different domain compositions with components of a basement membrane extract by immunoprecipitation. Strong binding of the LRR-containing construct was detected with the major ECM protein laminin. Analysis of these interactions by surface plasmon resonance spectroscopy revealed similar kinetics and affinities of binding of the LRR-containing construct to human and murine laminin-111, with calculated dissociation constants of 1.0 and 1.5 μM, respectively. The findings are discussed with respect to the recently published in-solution structures of the...
Source: Archives of Biochemistry and Biophysics - May 30, 2020 Category: Biochemistry Authors: Sevcnikar B, Schaffner I, Chuang CY, Gamon L, Paumann-Page M, Hofbauer S, Davies MJ, Furtmüller PG, Obinger C Tags: Arch Biochem Biophys Source Type: research

Structural and functional characterization of sticholysin III: A newly discovered actinoporin within the venom of the sea anemone Stichodactyla helianthus.
zo Á Abstract Actinoporins are a family of pore-forming toxins produced by sea anemones as part of their venomous cocktail. These proteins remain soluble and stably folded in aqueous solution, but when interacting with sphingomyelin-containing lipid membranes, they become integral oligomeric membrane structures that form a pore permeable to cations, which leads to cell death by osmotic shock. Actinoporins appear as multigenic families within the genome of sea anemones: several genes encoding very similar actinoporins are detected within the same species. The Caribbean Sea anemone Stichodactyla helianthus pr...
Source: Archives of Biochemistry and Biophysics - May 30, 2020 Category: Biochemistry Authors: Rivera-de-Torre E, Palacios-Ortega J, Garb JE, Slotte JP, Gavilanes JG, Martínez-Del-Pozo Á Tags: Arch Biochem Biophys Source Type: research

Role of carbonic anhydrases in ferroptosis-resistance.
Abstract Iron is essential for all the lives on earth but may trigger a switch toward ferroptosis, a novel form of regulated necrosis. Carbonic anhydrases (CAs) are ubiquitous enzymes from microbes to humans. The primary function of CAs is to regulate cellular pH by hydrating carbon dioxide (CO2) to protons (H+) and bicarbonate ions (HCO3-). Furthermore, CAs play roles in biosynthetic reactions, such as gluconeogenesis, lipogenesis, ureagenesis and are also associated with tumor metabolism, suggesting that CAs may be a potential target for the treatment of cancers. We have recently revealed a novel function of CA ...
Source: Archives of Biochemistry and Biophysics - May 30, 2020 Category: Biochemistry Authors: Li Z, Jiang L, Toyokuni S Tags: Arch Biochem Biophys Source Type: research

Biosynthesis of desferrioxamine siderophores initiated by decarboxylases: A functional investigation of two lysine/ornithine-decarboxylases from Gordonia rubripertincta CWB2 and Pimelobacter simplex 3E.
In this study we focused on the genes grdesA form Gordonia rubripertincta CWB2 and psdesA from Pimelobacter simplex VkMAC-2033D that encode decarboxylases presumed to be involved in the synthesis of desferrioxamine siderophores. The corresponding proteins GrDesA and PsDesA, were heterologously produced in Escherichia coli and purified. PsDesA was isolated bound to the cofactor pyridoxal 5-phosphate and GrDesA was purified in its apo form. PsDesA showed a moderate substrate preference for lysine (Km = 0.17 mM, kcat = 0.26 s-1) compared to ornithine (Km = 0.13 mM, kcat = 0.14 s-1), while GrDesA exhibited ...
Source: Archives of Biochemistry and Biophysics - May 29, 2020 Category: Biochemistry Authors: Hofmann M, Martin Del Campo JS, Sobrado P, Tischler D Tags: Arch Biochem Biophys Source Type: research

Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.
CONCLUSION: Silencing of circ-FARSA restricted the growth of CRC through regulating the miR-330-5p/LASP1 axis, providing a novel regulatory mechanism for CRC tumorigenesis. PMID: 32473899 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 28, 2020 Category: Biochemistry Authors: Lu C, Fu L, Qian X, Dou L, Cang S Tags: Arch Biochem Biophys Source Type: research

Severity and duration of hypoxic stress differentially regulates HIF-1 α-mediated cardiomyocyte apoptotic signaling milieu during myocardial infarction.
CONCLUSION: ROS-mediated HIF-1α stabilization promotes cardiomyocyte apoptosis on one hand while NO-mediated stabilization of HIF-1α disrupts apoptosis depending upon the severity and duration of hypoxia. Therefore the outcome of modulation of cardiac HIF-1α activity is regulated by both the severity and duration of ischemic stress. PMID: 32473132 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 27, 2020 Category: Biochemistry Authors: Chaudhuri RD, Banerjee D, Banik A, Sarkar S Tags: Arch Biochem Biophys Source Type: research

Resveratrol suppresses gastric cancer cell proliferation and survival through inhibition PIM-1 kinase activity.
In conclusion, the proapototic and anti-proliferative effects of resveratrol in gastric cancer cells are likely to be mediated through suppression of PIM-1 kinase activity, which may represent a novel mechanism underlying its chemopreventive and anticarcinogenic actions. PMID: 32473133 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 27, 2020 Category: Biochemistry Authors: Kim S, Kim W, Kim DH, Jang JH, Kim SJ, Park SA, Hahn H, Han BW, Na HK, Chun KS, Choi BY, Surh YJ Tags: Arch Biochem Biophys Source Type: research

Black soybean improves the vascular function through an increase in nitric oxide and a decrease in oxidative stress in healthy women.
In this study, we investigated the effect of black soybean consumption on the vascular function and oxidative stress associating with the polyphenol concentrations in healthy women. Lowered vascular age was observed in 33 out of 44 volunteers who completed the 8-week trial. It was observed that improvement of the vascular stiffness, increasing in the urinary NO2 and NO3 level, and decreasing in the oxidative stress markers, 8-hydroxy-2'-deoxyguanosine, hexanoyl-lysine and myeloperoxidase. In addition, concentration of 12 polyphenols in black soybean increased in the plasma and urine. Increased concentration of polyphenols ...
Source: Archives of Biochemistry and Biophysics - May 26, 2020 Category: Biochemistry Authors: Yamashita Y, Wang L, Nakamura A, Nanba F, Saito S, Toda T, Nakagawa J, Ashida H Tags: Arch Biochem Biophys Source Type: research

Necroptosis in renal ischemia/reperfusion injury: A major mode of cell death?
Abstract Ischemic acute kidney injury (AKI) is a frequent complication resulting from a myriad of conditions that decrease effective arterial blood volume to the kidneys including myocardial ischemia, sepsis, and hypovolemia. Following acute ischemic insult, restoration of renal blood flow inevitably leads to the aggravation of renal injury due to a widely researched condition known as ischemia/reperfusion (I/R) injury. For decades, apoptosis and necrosis have been proposed as being the two cell death pathways responsible for the pathogenesis of renal ischemic AKI. There is recent evidence to show that necrosis co...
Source: Archives of Biochemistry and Biophysics - May 26, 2020 Category: Biochemistry Authors: Jun W, Benjanuwattra J, Chattipakorn SC, Chattipakorn N Tags: Arch Biochem Biophys Source Type: research

Aronia berry inhibits disruption of Caco-2 intestinal barrier function.
The objective of this work was to determine how aronia berry polyphenols and its microbial catabolites improve intestinal barrier function. Caco-2 cells were cultured on transwell plates and allowed differentiate to form a model intestinal barrier, having baseline transepithelial electrical resistance (TEER) ≥ 300 Ω cm2. Barrier function of differentiated Caco-2 cells was compromised by the addition of an inflammatory cocktail (IC: TNF-α, IL-1β, and IFN-γ to the basolateral media and lipopolysaccharide to the apical media). Polyphenol-rich aronia berry powder or individual polyphenols ...
Source: Archives of Biochemistry and Biophysics - May 25, 2020 Category: Biochemistry Authors: Valdez JC, Cho J, Bolling BW Tags: Arch Biochem Biophys Source Type: research

Non-thermal plasma-activated lactate solution kills U251SP glioblastoma cells in an innate reductive manner with altered metabolism.
Abstract Ringer's lactate solution irradiated by non-thermal plasma, comprised of radicals, electrons, and ions, is defined as plasma-activated lactate (PAL). PAL exhibited antitumor effects in glioblastoma U251SP cells, which we termed PAL-specific regulated cell death. In contrast to the oxidative stress condition typical of cells incubated in plasma-activated medium (PAM), U251SP cells treated with Ringer's lactate solution or PAL exhibited changes in intracellular metabolites that were reductive in the redox state, as measured by the ratio of oxidative/reductive glutathione concentrations. In the metabolomic p...
Source: Archives of Biochemistry and Biophysics - May 25, 2020 Category: Biochemistry Authors: Ishikawa K, Hosoi Y, Tanaka H, Jiang L, Toyokuni S, Nakamura K, Kajiyama H, Kikkawa F, Mizuno M, Hori M Tags: Arch Biochem Biophys Source Type: research

Lipids and phenylketonuria: Current evidences pointed the need for lipidomics studies.
Abstract Phenylketonuria (PKU) is the most prevalent inborn error of amino acid metabolism. The disease is due to the deficiency of phenylalanine (Phe) hydroxylase activity, which causes the accumulation of Phe. Early diagnosis through neonatal screening is essential for early treatment implementation, avoiding cognitive impairment and other irreversible sequelae. Treatment is based on Phe restriction in the diet that should be maintained throughout life. High dietary restrictions can lead to imbalances in specific nutrients, notably lipids. Previous studies in PKU patients revealed changes in levels of plasma/ser...
Source: Archives of Biochemistry and Biophysics - May 24, 2020 Category: Biochemistry Authors: Guerra IMS, Ferreira HB, Neves B, Melo T, Diogo LM, Domingues MR, Moreira ASP Tags: Arch Biochem Biophys Source Type: research

Phoenixin 14 inhibits ischemia/reperfusion-induced cytotoxicity in microglia.
Abstract The process of ischemia/reperfusion (IR) in ischemic stroke often leads to significant cell death and permanent neuronal damage. Safe and effective treatments are urgently needed to mitigate the damage caused by IR injury. The naturally occurring pleiotropic peptide phoenixin 14 (PNX-14) has recently come to light as a potential treatment for IR injury. In the present study, we examined the effects of PNX-14 on several key processes involved in ischemic injury, such as pro-inflammatory cytokine expression, oxidative stress, and the related cascade mediated through the toll-like receptor 4 (TLR4) pathway, ...
Source: Archives of Biochemistry and Biophysics - May 22, 2020 Category: Biochemistry Authors: Ma H, Su D, Wang Q, Chong Z, Zhu Q, He W, Wang W Tags: Arch Biochem Biophys Source Type: research

The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation -induced mitochondrial and renal injury.
Abstract Kidneys from deceased donors used for transplantation are placed in cold storage (CS) solution during the search for a matched recipient. However, CS induces mitochondrial and cellular injury, which exacerbates renal graft dysfunction, highlighting the need for therapeutic interventions. Using an in vitro model of renal CS, we recently reported that pharmacological activation of the mitochondrial BK channel (mitoBK) during CS protected against CS-induced mitochondrial injury and cell death. Here, we used an in vivo syngeneic rat model of renal CS (18 h) followed by transplantation (24 h reperfus...
Source: Archives of Biochemistry and Biophysics - May 21, 2020 Category: Biochemistry Authors: Shrum S, Tobacyk J, Lo S, Parajuli N, MacMillan-Crow LA Tags: Arch Biochem Biophys Source Type: research

Curcumin suppress glioblastoma cell proliferation by p-AKT/mTOR pathway and increased the PTEN expression.
CONCLUSIONS: These results indicated that curcumin inhibited proliferation by decreasing the p-AKT/p-mTOR pathway, and promoted apoptosis by increasing the PTEN and p53 expression. Our study provided the molecular mechanisms by which curcumin inhibited glioblastoma and its targeted interventions. PMID: 32445778 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 20, 2020 Category: Biochemistry Authors: Wang Z, Liu F, Liao W, Yu L, Hu Z, Li M, Xia H Tags: Arch Biochem Biophys Source Type: research

MicroRNA-27 attenuates pressure overload-Induced cardiac hypertrophy and dysfunction by targeting galectin-3.
In conclusion, MiR-27b be used to exert a protective role against cardiac dysfunction and hypertrophy by decreasing the expression level of galectin-3. The methodology suggested in this study provides a novel therapeutic strategy against cardiac hypertrophy. PMID: 32439330 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 18, 2020 Category: Biochemistry Authors: Zhang M, Cheng K, Chen H, Tu J, Shen Y, Pang L, Wu W Tags: Arch Biochem Biophys Source Type: research

MicroRNA-621 inhibits the growth of gastric cancer cells by targeting SYF2.
Abstract Increasing evidence indicates that aberrantly expressed microRNAs play a role in tumorigenesis and progression of gastric cancer. Recently, a novel cancer-related microRNA, miR-621, was found to be involved in cancer pathogenesis. However, the precise molecular mechanisms underlying the oncogenic activity of miR-621 remain unclear and require further investigation. In the current study, we demonstrate that miR-621 expression is downregulated in gastric cancer tissues and cell lines, and its reduction is associated with malignant clinical features including tumor size, lymph node metastasis, tumor-node-met...
Source: Archives of Biochemistry and Biophysics - May 14, 2020 Category: Biochemistry Authors: Tao Y, Zhao Y, Peng Y, Ma X, Sun C, Xu K Tags: Arch Biochem Biophys Source Type: research

IL-10 regulates the malignancy of hemangioma-derived endothelial cells via regulation of PCNA.
Abstract Hemangioma (HA) is the most common benign tumor and formed by the proliferating endothelial cells of blood vessels. Interleukins (ILs) have been reported to be critical for HA progression. Our present study found that the expression of IL-10 was decreased in HA cells and tissues as compared to their corresponding controls. Treatment with recombinant IL-10 (rIL-10) can suppress the proliferation of HA cells via suppression of proliferating cell nuclear antigen (PCNA), while over expression of PCNA can attenuate rIL-10-inhibited cell proliferation. Further, rIL-10 can decrease the promoter activity and mRNA...
Source: Archives of Biochemistry and Biophysics - May 13, 2020 Category: Biochemistry Authors: Zeng Z, Chen H, Cai J, Huang Y, Yue J Tags: Arch Biochem Biophys Source Type: research

Suppression of cathepsin a inhibits growth, migration, and invasion by inhibiting the p38 MAPK signaling pathway in prostate cancer.
This study aimed at investigating the correlation between the cathepsin A (CTSA) gene and prostate cancer. Our findings indicated a significantly elevated level of CTSA gene expression in the tissues of patients with prostate cancer when compared with normal prostate tissues. Furthermore, the knockdown of the CTSA gene in the representative prostate cancer cell lines PC3 and DU145 led to reduced proliferation and a marked reduction in anchorage-independent colony formation, which was shown to be caused by cell cycle arrest in the S phase. In addition, CTSA gene-knockdown prostate cancer cell lines showed a substantial decr...
Source: Archives of Biochemistry and Biophysics - May 11, 2020 Category: Biochemistry Authors: Park S, Kwon W, Park JK, Baek SM, Lee SW, Cho GJ, Ha YS, Lee JN, Kwon TG, Kim MO, Ryoo ZY, Han SH, Han JE, Choi SK Tags: Arch Biochem Biophys Source Type: research

AK002210 promotes the proliferation, migration and invasion of trophoblast cell through regulating miR-590/NAIP signal axis.
In conclusion, we found that AK002210 knockdown may play a critical role in the progression of PE via miR-590-3p/NAIP and ERK/MMP signaling. It has potential to be a novel prognostic or therapeutic marker of PE. PMID: 32387473 [PubMed - as supplied by publisher] (Source: Archives of Biochemistry and Biophysics)
Source: Archives of Biochemistry and Biophysics - May 6, 2020 Category: Biochemistry Authors: Zhao L, Liang X, Ma Y, Li J, Liao S, Chen J, Wang C Tags: Arch Biochem Biophys Source Type: research

Characterization of hydroxymethylpyrimidine phosphate kinase from mesophilic and thermophilic bacteria and structural insights into their differential thermal stability.
dez V Abstract The hydroxymethylpyrimidine phosphate kinases (HMPPK) encoded by the thiD gene and involved in the thiamine biosynthesis pathway, can perform two consecutive phosphorylations of 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) and are found in thermophilic and mesophilic bacteria, and only a few characterizations of mesophilic enzymes are available. The presence of another homolog enzyme (pyridoxal kinase) that can only catalyze the first phosphorylation of HMP and encoded by pdxK gene, has hampered a precise annotation in this enzyme family. Here we report the kinetic characterization of two HMPPK...
Source: Archives of Biochemistry and Biophysics - May 5, 2020 Category: Biochemistry Authors: Cea PA, Araya G, Vallejos G, Recabarren R, Alzate-Morales J, Babul J, Guixé V, Castro-Fernandez V Tags: Arch Biochem Biophys Source Type: research

The high-resolution crystal structure of lobster hemocyanin shows its enzymatic capability as a phenoloxidase.
Abstract Hemocyanin (Hc) and phenoloxidase (PO) are the members of type 3 copper protein family. Although arthropod Hc and PO exhibit similar three-dimensional structures of the copper-containing active site, Hc functions as an oxygen transport protein, showing minimal or no phenoloxidase activity. Here, we present the crystal structure of the oxy form of Hc from Panulirus japonicus (PjHc) at 1.58 Å resolution. The structure of the di-copper active site of PjHc was found to be almost identical to that of PO. Although conserved amino acids and the water molecule crucial for the enzymatic activity were obser...
Source: Archives of Biochemistry and Biophysics - May 4, 2020 Category: Biochemistry Authors: Masuda T, Baba S, Matsuo K, Ito S, Mikami B Tags: Arch Biochem Biophys Source Type: research