Isobavachin attenuates osteoclastogenesis and periodontitis-induced bone loss by inhibiting cellular iron accumulation and mitochondrial biogenesis
Biochem Pharmacol. 2024 Apr 12:116202. doi: 10.1016/j.bcp.2024.116202. Online ahead of print.ABSTRACTAs bone-resorbing cells rich in mitochondria, osteoclasts require high iron uptake to promote mitochondrial biogenesis and maintain a high-energy metabolic state for active bone resorption. Given that abnormal osteoclast formation and activation leads to imbalanced bone remodeling and osteolytic bone loss, osteoclasts may be crucial targets for treating osteolytic diseases such as periodontitis. Isobavachin (IBA), a natural flavonoid compound, has been confirmed to be an inhibitor of receptor activator of nuclear factor κB...
Source: Biochemical Pharmacology - April 14, 2024 Category: Drugs & Pharmacology Authors: Ting Li Yangge Du Hantao Yao Boxuan Zhao Zijun Wang Rourong Chen Yaoting Ji Minquan Du Source Type: research
Nanomaterials-induced programmed cell death: Focus on mitochondria
Toxicology. 2024 Apr 12:153803. doi: 10.1016/j.tox.2024.153803. Online ahead of print.ABSTRACTNanomaterials are widely utilized in several domains, such as everyday life, societal manufacturing, and biomedical applications, which expand the potential for nanomaterials to penetrate biological barriers and interact with cells. Multiple studies have concentrated on the particular or improper utilization of nanomaterials, resulting in cellular death. The primary mode of cell death caused by nanotoxicity is programmable cell death, which includes apoptosis, ferroptosis, necroptosis, and pyroptosis. Based on our prior publicatio...
Source: Toxicology - April 14, 2024 Category: Toxicology Authors: Shijia Qiao Yiyuan Kang Xiner Tan Xinru Zhou Can Zhang Shulin Lai Jia Liu Longquan Shao Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
Isobavachin attenuates osteoclastogenesis and periodontitis-induced bone loss by inhibiting cellular iron accumulation and mitochondrial biogenesis
Biochem Pharmacol. 2024 Apr 12:116202. doi: 10.1016/j.bcp.2024.116202. Online ahead of print.ABSTRACTAs bone-resorbing cells rich in mitochondria, osteoclasts require high iron uptake to promote mitochondrial biogenesis and maintain a high-energy metabolic state for active bone resorption. Given that abnormal osteoclast formation and activation leads to imbalanced bone remodeling and osteolytic bone loss, osteoclasts may be crucial targets for treating osteolytic diseases such as periodontitis. Isobavachin (IBA), a natural flavonoid compound, has been confirmed to be an inhibitor of receptor activator of nuclear factor κB...
Source: Biochemical Pharmacology - April 14, 2024 Category: Drugs & Pharmacology Authors: Ting Li Yangge Du Hantao Yao Boxuan Zhao Zijun Wang Rourong Chen Yaoting Ji Minquan Du Source Type: research
Overexpression of Rhodiola crenulata glutathione peroxidase 5 increases cold tolerance and enhances the pharmaceutical value of the hairy roots
In this study, we investigated the role of GPX5 in Rhodiola crenulata's cold tolerance mechanisms. By overexpressing Rhodiola crenulata GPX5 (RcGPX5) in yeast and Arabidopsis thaliana, we observed down-regulation of Arabidopsis thaliana GPX5 (AtGPX5) and increased cold tolerance in both organisms. Furthermore, the levels of antioxidants and enzyme activities in the ascorbate-glutathione cycle were elevated, and cold-responsive genes such as AtCBFs and AtCORs were induced. Additionally, RcGPX5 overexpressing lines showed insensitivity to exogenous abscisic acid (ABA), suggesting a negative regulation of the ABA pathway by R...
Source: Gene - April 14, 2024 Category: Genetics & Stem Cells Authors: Kai Hou Lu Cao Wen Li Zhi-Hui Fang Daqiang Sun Zhigang Guo Lipeng Zhang Source Type: research
Hepatocyte mitochondrial DNA mediates macrophage immune response in liver injury induced by trichloroethylene
Ecotoxicol Environ Saf. 2024 Apr 13;276:116317. doi: 10.1016/j.ecoenv.2024.116317. Online ahead of print.ABSTRACTWe have previously shown that excessive activation of macrophage proinflammatory activity plays a key role in TCE-induced immune liver injury, but the mechanism of polarization is unclear. Recent studies have shown that TLR9 activation plays an important regulatory role in macrophage polarization. In the present study, we demonstrated that elevated levels of oxidative stress in hepatocytes mediate the release of mtDNA into the bloodstream, leading to the activation of TLR9 in macrophages to regulate macrophage p...
Source: Ecotoxicology and Environmental Safety - April 14, 2024 Category: Environmental Health Authors: Lei Gao Xu-Lei Zuo Luo-Lun Dong Si-Fan Zhou Zhou-Jian Wang Yuan-Sheng Duan Mu-Yue Chen Qi-Xing Zhu Jia-Xiang Zhang Source Type: research
Nanomaterials-induced programmed cell death: Focus on mitochondria
Toxicology. 2024 Apr 12:153803. doi: 10.1016/j.tox.2024.153803. Online ahead of print.ABSTRACTNanomaterials are widely utilized in several domains, such as everyday life, societal manufacturing, and biomedical applications, which expand the potential for nanomaterials to penetrate biological barriers and interact with cells. Multiple studies have concentrated on the particular or improper utilization of nanomaterials, resulting in cellular death. The primary mode of cell death caused by nanotoxicity is programmable cell death, which includes apoptosis, ferroptosis, necroptosis, and pyroptosis. Based on our prior publicatio...
Source: Toxicology - April 14, 2024 Category: Toxicology Authors: Shijia Qiao Yiyuan Kang Xiner Tan Xinru Zhou Can Zhang Shulin Lai Jia Liu Longquan Shao Source Type: research
Health risk assessment of heavy metal(loid)s in road dust via dermal exposure pathway from a low latitude plateau provincial capital city: The importance of toxicological verification
In this study, the road dust samples were harvested from a typical low-latitude plateau provincial capital city Kunming, Southwest China. The total concentration and dermal bioaccessibility of heavy metal(loid)s in road dust were determined, and their health risks and cytotoxicity on human skin keratinocytes were also assessed. The average concentrations of As (28.5 mg/kg), Cd (2.65 mg/kg), Mn (671 mg/kg), and Zn (511 mg/kg) exceeded the soil background values. Arsenic had the highest bioaccessibility after 2 h (3.79%), 8 h (4.24%), and 24 h (16.6%) extraction. Although soil assessment methods showed no health risk in the ...
Source: Environmental Research - April 14, 2024 Category: Environmental Health Authors: Jingya Li Daolei Cui Ziyue Yang Jiaoyang Ma Jianjun Liu Yingxin Yu Xianfeng Huang Ping Xiang Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
RNF31 alleviates liver steatosis by promoting p53/BNIP3-related mitophagy in hepatocytes
CONCLUSIONS: The findings highlight RNF31's essential role in the regulation of mitochondrial homeostasis in hepatocytes, emphasizing its potential as a therapeutic target for NAFLD.PMID:38615890 | DOI:10.1016/j.freeradbiomed.2024.04.214 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - April 14, 2024 Category: Biology Authors: Yifei Chen Fuji Yang Yujie Shi Jingyu Sheng Yanjin Wang Liting Zhang Jing Zhou Yi Jin Yongmin Yan Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
RNF31 alleviates liver steatosis by promoting p53/BNIP3-related mitophagy in hepatocytes
CONCLUSIONS: The findings highlight RNF31's essential role in the regulation of mitochondrial homeostasis in hepatocytes, emphasizing its potential as a therapeutic target for NAFLD.PMID:38615890 | DOI:10.1016/j.freeradbiomed.2024.04.214 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - April 14, 2024 Category: Biology Authors: Yifei Chen Fuji Yang Yujie Shi Jingyu Sheng Yanjin Wang Liting Zhang Jing Zhou Yi Jin Yongmin Yan Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
C-terminal region of Rv1039c (PPE15) protein of Mycobacterium tuberculosis targets host mitochondria to induce macrophage apoptosis
This study investigated the role of C-terminal region of Rv1039c (PPE15) in inducing mitochondrial perturbations and macrophage apoptosis. Ourin-silico studies revealed the disordered, coiled, and hydrophobic C-terminal region in Rv1039c has similarity with C-terminal of mitochondria-targeting pro-apoptotic host proteins. Wild type Rv1039c and C-terminal deleted Rv1039c (Rv1039c-/-Cterm) recombinant proteins were purified and theirM. smegmatis knock-in strains were constructed which were used forin-vitro experiments. Confocal microscopy showed localization of Rv1039c to mitochondria of PMA-differentiated THP1 macrophages; ...
Source: Apoptosis - April 14, 2024 Category: Molecular Biology Source Type: research
