BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research
C-terminal region of Rv1039c (PPE15) protein of Mycobacterium tuberculosis targets host mitochondria to induce macrophage apoptosis
This study investigated the role of C-terminal region of Rv1039c (PPE15) in inducing mitochondrial perturbations and macrophage apoptosis. Ourin-silico studies revealed the disordered, coiled, and hydrophobic C-terminal region in Rv1039c has similarity with C-terminal of mitochondria-targeting pro-apoptotic host proteins. Wild type Rv1039c and C-terminal deleted Rv1039c (Rv1039c-/-Cterm) recombinant proteins were purified and theirM. smegmatis knock-in strains were constructed which were used forin-vitro experiments. Confocal microscopy showed localization of Rv1039c to mitochondria of PMA-differentiated THP1 macrophages; ...
Source: Apoptosis - April 14, 2024 Category: Molecular Biology Source Type: research
The mechanisms of mitochondrial abnormalities that contribute to sleep disorders and related neurodegenerative diseases
Ageing Res Rev. 2024 Apr 11:102307. doi: 10.1016/j.arr.2024.102307. Online ahead of print.ABSTRACTSleep is a highly intricate biological phenomenon, and its disorders play a pivotal role in numerous diseases. However, the specific regulatory mechanisms remain elusive. In recent years, the role of mitochondria in sleep disorders has gained considerable attention. Sleep deprivation not only impairs mitochondrial morphology but also decreases the number of mitochondria and triggers mitochondrial dysfunction. Furthermore, mitochondrial dysfunction has been implicated in the onset and progression of various sleep disorder-relat...
Source: Ageing Research Reviews - April 13, 2024 Category: Genetics & Stem Cells Authors: Wentao Zhang Dan Liu Mei Yuan Ling-Qiang Zhu Source Type: research
Design of a prodrug photocage for cancer cells detection and anticancer drug release
This article presents the design and synthesis of a novel Rhodamine B fluorescence probe. The design strategy involves utilizing an anticancer drug (Melphalan) to bind with a fluorescent group (HRhod-OH), forming HRhod-MeL, which is non-fluorescent. However, when exposed to the high levels of reactive oxygen species (ROS) of cancer cells, HRhod-MeL transforms into a red-emitting Photocage (Rhod-MeL), and selectively accumulates in the mitochondria of cancer cells, where, when activated with green light (556 nm), anti-cancer drugs released. The Photocage improve the efficacy of anti-cancer drugs and enables the precise diag...
Source: Talanta - April 13, 2024 Category: Chemistry Authors: Qianshan Shao Fei Zhang Chunxiao Li Yuyu Yang Shihan Liu Guang Chen Baolei Fan Source Type: research
The mechanisms of mitochondrial abnormalities that contribute to sleep disorders and related neurodegenerative diseases
Ageing Res Rev. 2024 Apr 11:102307. doi: 10.1016/j.arr.2024.102307. Online ahead of print.ABSTRACTSleep is a highly intricate biological phenomenon, and its disorders play a pivotal role in numerous diseases. However, the specific regulatory mechanisms remain elusive. In recent years, the role of mitochondria in sleep disorders has gained considerable attention. Sleep deprivation not only impairs mitochondrial morphology but also decreases the number of mitochondria and triggers mitochondrial dysfunction. Furthermore, mitochondrial dysfunction has been implicated in the onset and progression of various sleep disorder-relat...
Source: Ageing Research Reviews - April 13, 2024 Category: Genetics & Stem Cells Authors: Wentao Zhang Dan Liu Mei Yuan Ling-Qiang Zhu Source Type: research
Preface: Special issue: 14th International Conference on Brain Energy Metabolism: Energy substrates and microbiome govern brain bioenergetics and cognitive function with aging
This special issue, entitled “Energetics and cognition in aging brain: Impact of substrates and microbiome”, contains manuscripts from attendees at the 14th International Conference on Brain Energy Metabolism (ICBEM) held in Santa Fe, New Mexico, USA, October 24–27, 2022. Major topics include neuronal and mitochondrial en ergetics, higher brain functions involving astrocyte-neuron interactions, unique roles for glycogen, glycolytic upregulation during activation, determination of metabolite levels with genetically-encoded biosensors, dietary intervention in aging, gut-brain signaling, spreading depolarization and fun...
Source: Journal of Neurochemistry - April 13, 2024 Category: Neuroscience Authors: Mary C. McKenna,
In ‐Young Choi,
Arne Schousboe Tags: REVIEW Source Type: research
MARCH5 promotes aerobic glycolysis to facilitate ovarian cancer progression via ubiquitinating MPC1
In this study, we demonstrate that the abnormal upregulation of MARCH5 is accompanied by significantly increased aerobic glycolysis in OC. Mechanistically, MARCH5 promotes aerobic glycolysis via ubiquitinating and degrading mitochondrial pyruvate carrier 1 (MPC1), which mediates the transport of cytosolic pyruvate into mitochondria by localizing on mitochondria outer membrane. In line with this, MPC1 expression is significantly decreased and its downregulation is closely correlated with unfavorable survival. Furthermore, in vitro and in vivo assays revealed that MARCH5 upregulation-enhanced aerobic glycolysis played a crit...
Source: Apoptosis - April 13, 2024 Category: Molecular Biology Source Type: research
Influence of flavonoids from Sedum aizoon L. on mitochondrial function of Rhizopus nigricans in strawberry
In this study, the minimum inhibitory concentration (MIC) of FSAL onR. nigricans and cell apoptosis tests were studied to explore the inhibitory effect of FSAL onR. nigricans. The effects of FSAL on mitochondria ofR. nigricans were investigated through the changes of mitochondrial permeability transition pore(mPTP), mitochondrial membrane potential(MMP), Ca2+ content, H2O2 content, cytochromec (Cyt c) content, the related enzyme activity and related genes of mitochondria. The results showed that the MIC of FSAL onR. nigricans was 1.800 mg/mL, with the addition of FSAL (1.800 mg/mL), the mPTP openness ofR. nigricans incr...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research
MARCH5 promotes aerobic glycolysis to facilitate ovarian cancer progression via ubiquitinating MPC1
In this study, we demonstrate that the abnormal upregulation of MARCH5 is accompanied by significantly increased aerobic glycolysis in OC. Mechanistically, MARCH5 promotes aerobic glycolysis via ubiquitinating and degrading mitochondrial pyruvate carrier 1 (MPC1), which mediates the transport of cytosolic pyruvate into mitochondria by localizing on mitochondria outer membrane. In line with this, MPC1 expression is significantly decreased and its downregulation is closely correlated with unfavorable survival. Furthermore, in vitro and in vivo assays revealed that MARCH5 upregulation-enhanced aerobic glycolysis played a crit...
Source: Apoptosis - April 13, 2024 Category: Molecular Biology Source Type: research
Energy metabolism as therapeutic target for aged wound repair by engineered extracellular vesicle
Sci Adv. 2024 Apr 12;10(15):eadl0372. doi: 10.1126/sciadv.adl0372. Epub 2024 Apr 12.ABSTRACTAging skin, vulnerable to age-related defects, is poor in wound repair. Metabolic regulation in accumulated senescent cells (SnCs) with aging is essential for tissue homeostasis, and adequate ATP is important in cell activation for aged tissue repair. Strategies for ATP metabolism intervention hold prospects for therapeutic advances. Here, we found energy metabolic changes in aging skin from patients and mice. Our data show that metformin engineered EV (Met-EV) can enhance aged mouse skin repair, as well as ameliorate cellular senes...
Source: Adv Data - April 12, 2024 Category: Epidemiology Authors: Yu Zhuang Shengjie Jiang Xiaoling Deng An Lao Xiaolin Hua Yun Xie Lingyong Jiang Xudong Wang Kaili Lin Source Type: research
Evaluation of Mitochondrial Phagy (Mitophagy) in Human Non-small Adenocarcinoma Tumor Cells
This study not only sheds light on the significance of mitophagy in NSCLC but also establishes a foundational approach for its investigation, paving way for future research in this critical area of cancer biology.PMID:38607594 | DOI:10.1007/7651_2024_532 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 12, 2024 Category: Molecular Biology Authors: Javad Alizadeh Simone C da Silva Rosa Marco Cordani Saeid Ghavami Source Type: research
Membraneless and Membrane-bound Organelles in an Anhydrobiotic Cell Line are Protected from Desiccation-induced Damage
This study offers several exciting avenues for future studies in the animal model and Pv11 cell line that will further our understanding of anhydrobiosis and may lead to advancements in storing sensitive biologics at ambient temperatures for months or years.PMID:38608858 | DOI:10.1016/j.cstres.2024.04.002 (Source: Cell Stress and Chaperones)
Source: Cell Stress and Chaperones - April 12, 2024 Category: Cytology Authors: Clinton J Belott Oleg A Gusev Takahiro Kikawada Michael A Menze Source Type: research
Ferroptosis contributes to airway epithelial E-cadherin disruption in a mixed granulocytic asthma mouse model
This study was aimed to evaluate the role of ferroptosis and to investigate whether ferroptosis mediates E-cadherin disruption in mixed granulocyte asthma (MGA). Two murine models of MGA were established using toluene diisocyanate (TDI) or ovalbumin with Complete Freund's Adjuvant (OVA/CFA). Specific antagonists of ferroptosis, including Liproxstatin-1 (Lip-1) and Ferrostatin-1 (Fer-1) were given to the mice. The allergen-exposed mice displayed markedly shrunk mitochondria in the airway epithelia, with decreased volume and denser staining accompanied by down-regulated GPX4 as well as up-regulated FTH1 and malondialdehyde, ...
Source: Experimental Cell Research - April 12, 2024 Category: Cytology Authors: Sudan Gan Liqin Lin Zemin Chen Hailing Zhang Haixiong Tang Changyun Yang Jing Li Shiyue Li Lihong Yao Source Type: research
