Influence of flavonoids from Sedum aizoon L. on mitochondrial function of Rhizopus nigricans in strawberry

In this study, the minimum inhibitory concentration (MIC) of FSAL onR. nigricans and cell apoptosis tests were studied to explore the inhibitory effect of FSAL onR. nigricans. The effects of FSAL on mitochondria ofR. nigricans were investigated through the changes of mitochondrial permeability transition pore(mPTP), mitochondrial membrane potential(MMP), Ca2+ content, H2O2 content, cytochromec (Cyt c) content, the related enzyme activity and related genes of mitochondria. The results showed that the MIC of FSAL onR. nigricans was 1.800  mg/mL, with the addition of FSAL (1.800 mg/mL), the mPTP openness ofR. nigricans increased and the MMP reduced. Resulting in an increase in Ca2+ content, accumulation of H2O2 content and decrease ofCyt c content, the activity of related enzymes was inhibited and related genes were up-regulated (VDAC1, ANT) or down-regulated (SDHA, NOX2). This suggests that FSAL may achieve the inhibitory effect of fungi by damaging mitochondria, thereby realizing the postharvest freshness preservation of strawberries. This lays the foundation for the development of a new plant-derived antimicrobial agent.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research