Diversity of sediment associated Planctomycetes and its related phyla with special reference to anammox bacterial community in a high Arctic fjord
AbstractThe fjords of west Spitsbergen Svalbard, Arctic Norway, are undergoing a transformation as the impact of nutrient rich warmer Atlantic water is significantly altering the primary production and subsequently the carbon pool. Members of the phylumPlanctomycetes are ubiquitous in marine systems and are important in the mineralization of organic matter. Hence, the phylogenetic diversity and distribution pattern ofPlanctomycetes in the surface sediments of a high Arctic fjord, the Kongsfjorden were studied. Further, considering the release of ammonium as a part of mineralization, the diversity of bacterial community inv...
Source: World Journal of Microbiology and Biotechnology - July 8, 2020 Category: Microbiology Source Type: research

High-level soluble expression of human Cu,Zn superoxide dismutase with high activity in Escherichia coli
This study has opened up an effective avenue for industrial production of hCu,Zn-SOD through microbial fermentation in the future. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - July 8, 2020 Category: Microbiology Source Type: research

Microalgae harvesting by magnetic flocculation for biodiesel production: current status and potential
AbstractWith the increasing demand for energy, microalgae, as one of the promising feedstocks of biodiesel, has raised great awareness. Because of its small size, similar density to water and electrical stability, harvesting methods of microalgae that have low energy consumption and that are highly efficient, easy to large-scale and environmentally friendly have become a bottleneck restricting development of the whole process. Among the numerous possible harvesting methods, magnetic flocculation has the advantages of simple operation, fast separation and energy saving and thus is considered as a promising novel harvesting ...
Source: World Journal of Microbiology and Biotechnology - July 7, 2020 Category: Microbiology Source Type: research

Protective effects of Lactobacillus fermentum U-21 against paraquat-induced oxidative stress in Caenorhabditis elegans and mouse models
AbstractThe aims of this work were to identify in vivo manifestations of antioxidant activity ofLactobacillus strains isolated from healthy human biotopes and to show the possibility of protective action of the selected strain on the model of oxidative stress induced by paraquat in the model of early Parkinson ’s disease (PD) in mice. We studied the protective effects of 14Lactobacillus strains belonging to five species on the lifespan of the soil nematodeCaenorhabditis elegans experiencing oxidative stress induced by paraquat. TheLactobacillus strains used in this study were selected previously based on their abilit...
Source: World Journal of Microbiology and Biotechnology - July 6, 2020 Category: Microbiology Source Type: research

Application of machine learning algorithm and modified high resolution DNA melting curve analysis for molecular subtyping of Salmonella isolates from various epidemiological backgrounds in northern Thailand
In conclusion, the rapid, robust and specificSalmonella subtyping developed in the study can be performed in a local setting, enabling swift control and preventive measures to be initiated against potential epidemics of salmonellosis. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - July 2, 2020 Category: Microbiology Source Type: research

Natural antioxidant pterostilbene as an effective antibiofilm agent, particularly for gram-positive cocci
AbstractPterostilbene (PTE), a dimethylated analogue of resveratrol, mostly contained inVitis vinifera leaves or in other plant sources is well-known for its antioxidant activity. Due to its bioavailability, low hydrophilicity and thus ability to penetrate hydrophobic biological membranes it was found to be an antimicrobial agent. These properties of PTE offer the possibility of its use in the treatment of microbial infections. The emergence of antibiotic resistance of microorganisms is often caused by their ability to form biofilm; new substances with antibiofilm activity are therefore sought. The representatives of oppor...
Source: World Journal of Microbiology and Biotechnology - July 1, 2020 Category: Microbiology Source Type: research

Colistin heteroresistance in carbapenem-resistant Acinetobacter baumannii clinical isolates from a Thai university hospital
AbstractColistin is the last resort for the treatment of infections with carbapenem-resistant (CR) Gram-negative bacteria particularlyAcinetobacter baumannii (CRAB). Currently, both colistin-resistant and -heteroresistantA. baumannii isolates have been reported globally. We therefore investigated the colistin heteroresistance rate in 75 non-duplicate colistin-susceptible CRAB clinical isolates from a Thai university collected in 2016. Minimum inhibitory concentrations (MICs) of colistin for all isolates were determined by broth microdilution method and carbapenemase genes were detected by PCR methods. All isolates were gen...
Source: World Journal of Microbiology and Biotechnology - July 1, 2020 Category: Microbiology Source Type: research

A review of the nature, role and control of lithobionts on stone cultural heritage: weighing-up and managing biodeterioration and bioprotection
AbstractLithobionts (rock-dwelling organisms) have been recognized as agents of aesthetic and physico-chemical deterioration of stonework. In consequence, their removal from cultural heritage stone surfaces (CHSS) is widely considered a necessary step in conservation interventions. On the other hand, lithobiontic communities, including microbial biofilms ( ‘biological patinas’), can help integrate CHSS with their environmental setting and enhance biodiversity. Moreover, in some cases bioprotective effects have been reported and even interpreted as potential biotechnological solutions for conservation. This pape...
Source: World Journal of Microbiology and Biotechnology - July 1, 2020 Category: Microbiology Source Type: research

Correction to: pH control and the production of extracellular dextransucrase by Leuconostoc mesenteroides
Unfortunately, M. M. Vrvi ć name has been published incorrectly in the original publication as M. M. Vrivić, corrected name appears in this erratum. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - June 30, 2020 Category: Microbiology Source Type: research

Disruption and overexpression of 6-phosphofructo-2-kinase influence organic acid production in Aspergillus carbonarius ITEM 5010
In this study, we identified a genef2kp encoding the enzyme 6-phosphofructo-2-kinase known as an allosteric regulator of the glycolytic flux and investigated its role in the production of organic acid. The strategy was to examine the impact of citric acid and malic acid production by overexpressing and disruptingf2kp, respectively. The overexpressing transformants expressedf2kp at higher level than the wild type, whereas no expression off2kp was detected in the knockout transformants. Citric acid and malic acid production by the knockout strains decreased sharply along with a significant lower sugar consumption, though the...
Source: World Journal of Microbiology and Biotechnology - June 29, 2020 Category: Microbiology Source Type: research

Genomic and biotechnological insights on stress-linked polyphosphate production induced by chromium(III) in Ochrobactrum anthropi DE2010
AbstractThe resistance of microorganisms to heavy metals in polluted environments is mediated by genetically determined mechanisms. One such mechanism includes the intracellular sequestration of heavy metals in polyphosphate (polyP) inclusions. In Cr(III) contaminated mediums,Ochrobactrum anthropi DE2010 is able to bind and sequester Cr(III) in polyP inclusions. In order to further study the relationship between Cr(III) tolerance and polyP production inO. anthropi DE2010, we carried out whole genomic sequencing, analysis of single nucleotide polymorphisms (SNPs), polyP chemical quantification, and determination of the rela...
Source: World Journal of Microbiology and Biotechnology - June 26, 2020 Category: Microbiology Source Type: research

CRISPR –Cas9/CRISPRi tools for cell factory construction in E. coli
AbstractThe innovative CRISPR –Cas based genome editing technology provides some functionality and advantages such as the high efficiency and specificity as well as ease of handling. Both aspects of the CRISPR–Cas9 system including genetic engineering and gene regulation are advantageously applicable to the construction of m icrobial cell factories. As one of the most extensively used cell factories,E. coli has been engineered to produce various high value-added chemical compounds such as pharmaceuticals, biochemicals, and biofuels. Therefore, to improve the production of valuable metabolites, many investigatio...
Source: World Journal of Microbiology and Biotechnology - June 25, 2020 Category: Microbiology Source Type: research

Spent brewer ’s yeast as a source of high added value molecules: a systematic review on its characteristics, processing and potential applications
AbstractDevelopment of new strategies to add-value to agro-industrial by-products are of environmental and economical importance. Innovative and low-cost sources of protein and bioactive peptides have been explored worldwide. Spent brewer ’s yeast (SBY) is the second most relevant by-product from the brewing industry, and despite its nutritional (about 50% protein, dry weight) and technological potential, it is still underused or needs to be disposed of. SBY cells need to be disrupted to release intracellular and cell wall proteins . This procedure has been performed using autolysis, glass bead milling, enzymatic hyd...
Source: World Journal of Microbiology and Biotechnology - June 24, 2020 Category: Microbiology Source Type: research

A transcriptome analysis of the antibacterial mechanism of flavonoids from Sedum aizoon L. against Shewanella putrefaciens
This study provided new insights into the anti-S. putrefaciens molecular mechanism underlying the treatment of FSAL, which may be served as the basis guide for identifying potential antimicrobial targets and application of FSAL in food safety.Graphic abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - June 20, 2020 Category: Microbiology Source Type: research

Integration of molecular tools in microbial phosphate solubilization research in agriculture perspective
AbstractPhosphorus (P) is the second most crucial nutrient for plant growth after nitrogen. However, its highly reactive nature causes formation of insoluble derivatives and limits uptake by the plant roots. The wide spread applications of P based chemical fertilizers cause detrimental effects on soil fertility, agricultural product quality and environment. In this regard, phosphate-solubilizing microorganisms (PSMs) stand out as the most remarkable and promising tools for the development of safer and sustainable technologies. As a result of this, many bacterial and fungal species with significant phosphate-solubilizing ac...
Source: World Journal of Microbiology and Biotechnology - June 20, 2020 Category: Microbiology Source Type: research

Molecular mechanisms in grass- Epichlo ë interactions: towards endophyte driven farming to improve plant fitness and immunity
AbstractAll plants harbor many microbial species including bacteria and fungi in their tissues. The interactions between the plant and these microbes could be symbiotic, mutualistic, parasitic or commensalistic. Mutualistic microorganisms are endophytic in nature and are known to play a role in plant growth, development and fitness. Endophytes display complex diversity depending upon the agro-climatic conditions and this diversity could be exploited for crop improvement and sustainable agriculture. Plant-endophyte partnerships are highly specific, several genetic and molecular cascades play a key role in colonization of en...
Source: World Journal of Microbiology and Biotechnology - June 19, 2020 Category: Microbiology Source Type: research

Isolation and molecular identification of Aspergillus flavus and the study of its potential for malathion biodegradation in water
This study was carried out for isolation and molecular identification of microorganisms from water for malathion biodegradation in aquatic system. PCR analysis was used for identification of the isolated fungus. The growth kinetics ofA. flavus in the presence of malathion under different environmental factors (pH, temperature and malathion concentration) were evaluated. Furthermore, the degradation kinetics of malathion byA. flavus in aqueous media under different environmental factors was evaluated. The isolated microorganism was identified asA. flavus with respect to it relation to the data from the gene bank and the low...
Source: World Journal of Microbiology and Biotechnology - June 18, 2020 Category: Microbiology Source Type: research

Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167
This study highlights the antifungal and nematicidal activity of chitinases produced byChitinophaga sp. S167. To the best of our knowledge, this is the first report of the biocontrol potential of the chitinases produced byChitinophaga sp. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - June 11, 2020 Category: Microbiology Source Type: research

The roles and applications of chaotropes and kosmotropes in industrial fermentation processes
AbstractChaotropicity has long been recognised as a property of some compounds. Chaotropes tend to disrupt non-covalent interactions in biological macromolecules (e.g. proteins and nucleic acids) and supramolecular assemblies (e.g. phospholipid membranes). This results in the destabilisation and unfolding of these macromolecules and assemblies. Unsurprisingly, these compounds are typically harmful to living cells since they act against multiple targets, comprising cellular integrity and function. Kosmotropes are the opposite of chaotropes and these compounds promote the ordering and rigidification of biological macromolecu...
Source: World Journal of Microbiology and Biotechnology - June 8, 2020 Category: Microbiology Source Type: research

Production and investigation of the physico-chemical properties of MEL-A from glycerol and coconut water
AbstractThis work reports the production of MEL-A using coconut water as the carbon source. Proximate analysis of coconut water indicated the presence of nutrients necessary for growth of the organism and production of desired metabolite. The amount of MEL produced using coconut water was 3.85  g/L (± 0.35) with 74% of it being MEL-A when compared to 2.58 g/L (± 0.15) with 60% being MEL-A using glycerol, a conventional carbon source. MEL-A from coconut water consisted of 38.1% long-chain saturated fatty acids (C16:0 and C18:0) whereas with glycerol it was 9.6%. The critical micell ar c...
Source: World Journal of Microbiology and Biotechnology - June 5, 2020 Category: Microbiology Source Type: research

Biofertilizers with beneficial rhizobacteria improved plant growth and yield in chili ( Capsicum annuum L.)
This study provided a theoretical and practical basis for large scale development of integrated biofertilizers using beneficial rhizobacterial strains from the desert pla nt rhizosphere. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - June 2, 2020 Category: Microbiology Source Type: research

Microcosm based analysis of arsenic release potential of Bacillus sp. strain IIIJ3-1 under varying redox conditions
This study confirms that microbe-mediated mineral weathering followed by respiratory reduction of As facilitates mobilization of sediment hosted As into aqueous phase, and provides a better insight into the catabolic ability of groundwater bacteria in mobilization of sediment hosted As in BRB region.Graphic abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - June 2, 2020 Category: Microbiology Source Type: research

Transcriptional regulation of drug resistance mechanisms in Salmonella : where we stand and what we need to know
AbstractSalmonellae have evolved a wide range of molecular mechanisms to neutralize the effect of antibiotics and evade the host immune system response. These mechanisms are exquisitely controlled by global and local regulators and enable the pathogens to use its energy as per need and hence allow the pathogen to economize the consumption of energy by its cellular machinery. Several families that regulate the expression of different drug resistance genes are known; some of these are: the TetR family (which affects tetracycline resistance genes), the AraC/XylS family (regulators that can act as both transcriptional activato...
Source: World Journal of Microbiology and Biotechnology - May 29, 2020 Category: Microbiology Source Type: research

Functional characterization of the endolysins derived from mycobacteriophage PDRPxv
In this study, we report the structural and functional characterization of endolysins (LysinA and LysinB) encoded by mycobacteriophage PDRPxv which belongs to B1 sub cluster. On in silico analysis, we found LysinA to be a modular protein having peptidase domain at the N-terminal (104 aa), a central amidase domain (174 aa) and  the peptidoglycan binding domain (62 aa) at the C-terminal. Additionally, ‘H-X-H’, which is a conserved motif and characteristic of peptidase domains, and the conserved residues His-His-Asp, which are characteristic of amidase domain were also observed. In LysinB enzyme, a single &al...
Source: World Journal of Microbiology and Biotechnology - May 28, 2020 Category: Microbiology Source Type: research

Retraction Note to: A method of multiplex PCR for detection of field released Beauveria bassiana , a fungal entomopathogen applied for pest management in jute ( Corchorus olitorius )
The Editor-in-Chief has retracted this article [1] due to similarities between lanes 1-4 in Figures 1 and 2. These similarities have raised concerns about the reliability of the data presented. The authors, Chinmay Biswas, Piyali Dey, B. S. Gotyal& Subrata Satpathy have not responded to correspondence about this retraction. [1] Biswas, C., Dey, P., Gotyal, B.S. et al. A method of multiplex PCR for detection of field released Beauveria bassiana, a fungal entomopathogen applied for pest management in jute (Corchorus olitorius). World J Microbiol Biotechnol 31, 675 -679 (2015). https://doi.org/10.1007/s11274-015-1821-...
Source: World Journal of Microbiology and Biotechnology - May 28, 2020 Category: Microbiology Source Type: research

Deciphering the crucial roles of AraC-type transcriptional regulator Cgl2680 on NADPH metabolism and l -lysine production in Corynebacterium glutamicum
AbstractLysine is widely used in food, medical and feed industries. The biosynthesis ofl-lysine is closely related to NADPH level, but the regulation mechanism between the biosynthesis ofl-lysine inC. glutamicum and the cofactor NADPH is still not clear. Here, a high intracellular NADPH level strainC. glutamicum XQ-5 Δpgi::(zwf-gnd) was constructed by blocking the glycolytic pathway and overexpressing the pentose phosphate pathway in the lysine-producing strainC. glutamicum XQ-5, and the intracellular NADPH level in strain XQ-5 Δpgi::(zwf-gnd) was increased from 3.57  × 10–5 nmol/...
Source: World Journal of Microbiology and Biotechnology - May 26, 2020 Category: Microbiology Source Type: research

Early reprecipitation of sulfate salts in coal biodesulfurization processes using acidophilic chemolithotrophic bacteria
This study evaluated the effect of three sulfate salt-based culture media on the reprecipitation of sulfur under the action of two types of bacterial inoculum, a pure strain ofAcidithiobacillus ferrooxidans (ATCC 23270) and a consortium of this strain andAcidithiobacillus thiooxidans (ATCC 15494), in a biodesulfurization process for coal (particle size  
Source: World Journal of Microbiology and Biotechnology - May 25, 2020 Category: Microbiology Source Type: research

Effects of Spo0A on Clostridium acetobutylicum with an emphasis on biofilm formation
AbstractClostridium acetobutylicum is a well-known strain for biofuel production. In previous work, it was found that this strain formed biofilm readily during fermentation processes. Biofilm formation could protect cells and enhance productivities under environmental stresses in our previous work. To explore the molecular mechanism of biofilm formation, Spo0A ofC. acetobutylicum was selected to investigate its influences on biofilm formation and other physiological performances. Whenspo0A gene was disrupted, thespo0A mutant could hardly form biofilm. The aggregation and adhesion abilities of thespo0A mutant as well as its...
Source: World Journal of Microbiology and Biotechnology - May 22, 2020 Category: Microbiology Source Type: research

The role of endogenous and exogenous hydrogen in the microbiology of biogas production systems
This study also investigates (1) the role of endogenous hydrogen as an intermediate metabolite and of interspecies electron transfer in anaerobic digestion, (2) effects of exogenous hydrogen addition on microbial community structure and metabolic processes, and (3) recent developments regarding in-situ and ex-situ biogas upgrading systems via hydrogen addition. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - May 22, 2020 Category: Microbiology Source Type: research

Novel formulations of Bacillus thuringiensis var . kurstaki: an eco-friendly approach for management of lepidopteran pests
AbstractBacillus thuringiensis (Bt) and entomopathogenic fungi (EPF) are in use for management of insect pests. Continuous use of Bt can lead to problem of resistance development in insect pests. Hence use of combination formulations (CF) of microbials with diverse modes of action has been attempted to slow down the process of resistance development. Suspension concentrate (SC) formulations of a local strain of Bt var.kurstaki DOR Bt-127 were developed singly and in combination with conidia of the EPFNomuraea rileyi (Nr) andBeauveria bassiana (Bb). Electron microscopy of Bt  + Bb CF treated larvae ofHelicover...
Source: World Journal of Microbiology and Biotechnology - May 15, 2020 Category: Microbiology Source Type: research

Antimicrobial peptide (AMP) from Zingiber zerumbet rhizomes with inhibitory effect on Pythium myriotylum secretory proteases and zoospore viability
AbstractProtease mediated proteolysis has been widely implicated in virulence of necrotrophic fungal pathogens. This is counteracted in plants by evolving new and effective antimicrobial peptides (AMP) that constitute important components of innate immune system. Peptide extraction from rhizome ofZingiber zerumbet was optimized using ammonium sulphate (50 –80% w/v) and acetone (60 and 100% v/v) with maximal protein recovery of 1.2 ± 0.4 mg/g obtained using 100% acetone. Evaluation of inhibitory potential ofZ.zerumbet rhizome protein extract to prominent hydrolases of necrotrophicPythium myri...
Source: World Journal of Microbiology and Biotechnology - May 13, 2020 Category: Microbiology Source Type: research

Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye
In this study, loop-mediated isothermal amplification (LAMP) assay with the azo dye, hydroxynaphthol blue (HNB) was developed targeting species-specifictlh gene. The assay was carried out on 62 seafood samples that included clam and shrimp and compared with conventional LAMP assay performed with the commonly used fluorescent dye, conventional PCR, and real-time PCR (RT-PCR). Of 62 samples studied fortlh gene, 32 (51.61%) gave positive by HNB-LAMP, which comprised 22 (70.96%) clam samples and 10 (32.25%) shrimp samples. The HNB-LAMP assay was found to be highly sensitive, specific, and superior to conventional PCR (p&thinsp...
Source: World Journal of Microbiology and Biotechnology - May 10, 2020 Category: Microbiology Source Type: research

High-strength wastewater treatment using microbial biofilm reactor: a critical review
AbstractBiofilm reactors retain  microbial cells in the form of biofilm which is attached to free moving or fixed carrying materials, thus providing a high active biomass concentration and automatic liquid and solid separation. Nowadays, microbial biofilm reactors have been widely used in high-strength wastewater treatment wher e very high pollutant removal efficiency is required, which usually requires excessive space and aeration energy for conventional activated sludge-based treatment. This paper provides an overview of microbial biofilm reactors developed over the last half-...
Source: World Journal of Microbiology and Biotechnology - May 10, 2020 Category: Microbiology Source Type: research

Biodegradation of recalcitrant compounds and phthalates by culturable bacteria isolated from Liometopum apiculatum microbiota
AbstractLiometopum apiculatum is a species of ants widely distributed in arid and semi-arid ecosystems where there is a relative food shortage compared with tropical ecosystems.L. apiculatum has established an ecological balance involving symbiotic interactions, which have allowed them to survive through mechanisms that are still unknown. Therefore, the aim of this study was to explore the metabolic potential of isolated bacteria fromL. apiculatum using enzymatic activity assay and substrate assimilation. Results revealed a complex bacteria consortium belonging to Proteobacteria, Firmicutes, and Actinobacteria phylum. Most...
Source: World Journal of Microbiology and Biotechnology - May 9, 2020 Category: Microbiology Source Type: research

Isolation, characterization and anti-inflammatory mechanism of probiotics in lipopolysaccharide-stimulated RAW 264.7 macrophages
AbstractProbiotics are known to modulate gut microbiota, intestinal barrier function and host immune response, but due to the species and strain specific response their mechanisms are not clearly understood. Thus, the present study was designed to isolate, assess the anti-inflammatory potential and underlying modulatory mechanisms of indigenous probiotics in murine macrophage cell line, RAW 264.7. Forty lactic acid bacteria (LAB) were isolated from different sources and monitored for their anti-inflammatory potential against lipopolysaccharide (LPS) induced inflammatory stress employing RAW 264.7 cells. Among these isolate...
Source: World Journal of Microbiology and Biotechnology - May 9, 2020 Category: Microbiology Source Type: research

Characterization of purified antimicrobial peptide produced by Pediococcus pentosaceus LJR1, and its application in preservation of white leg shrimp
AbstractThe bacteriocinogenic lactic acid bacteriumPediococcus pentosaceus LJR1 isolated from rumen liquor of goat had strong anti-bacterial activity towardListeria monocytogenes in vitro. This antibacterial activity was lost on treatment with protease indicating that the bacteriocin is proteinaceous in nature. The bacteriocin LJR1 produced byP. pentosaceus was purified following a three step procedure consisting of ammonium sulphate precipitation, gel filtration chromatography and reverse phase-high performance liquid chromatography. The molecular weight of purified bacteriocin was determined to be 4.6  kDa using Tri...
Source: World Journal of Microbiology and Biotechnology - May 4, 2020 Category: Microbiology Source Type: research

Growth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis
AbstractThe cosmopolitan entomopathogenic and root endophytic fungusMetarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores ofMetarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and gro...
Source: World Journal of Microbiology and Biotechnology - April 28, 2020 Category: Microbiology Source Type: research

Effect of curcumin on the expression of ahyI / R quorum sensing genes and some associated phenotypes in pathogenic Aeromonas hydrophila fish isolates
This study demonstrated the use of curcumin to attenuateahyI/R QS genes and several QS associated phenotypes inA. hydrophila. These findings indicate the therapeutic potential of curcumin as an anti-QS agent, to be used againstA. hydrophila pathogenesis in aquaculture. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - April 28, 2020 Category: Microbiology Source Type: research

Inhibitory effects of aptamer targeted teicoplanin encapsulated PLGA nanoparticles for Staphylococcus aureus strains
In this study, we report teicoplanin delivery nanoparticles of Poly Lactic-co-Glycolic Acid (PLGA), which are functionalized withS. aureus specific aptamers. A 32-fold decrease in minimum inhibitory concentration (MIC) values of teicoplanin forS. aureus was demonstrated for susceptible strains and about 64-fold decline in MIC value was achieved for moderately resistant clinical isolates of MRSA upon teicoplanin treatment with aptamer-PLGA nanoparticles. Although teicoplanin delivery in PLGA nanoparticles without targeting demonstrated eightfold decrease in MIC of susceptible strains ofS. aureus andS. epidermidis and twofol...
Source: World Journal of Microbiology and Biotechnology - April 25, 2020 Category: Microbiology Source Type: research

Autolysis, plasmolysis and enzymatic hydrolysis of baker's yeast ( Saccharomyces cerevisiae ): a comparative study
AbstractSaccharomyces cerevisiae is being used for long as a rich source of proteins, sugars, nucleotides, vitamins and minerals. Autolyzed and hydrolyzed yeast biomass has found numerous applications in the health food industry as well as livestock feeds. Here, we have compared three lysis methods for production of yeast lysates using autolysis, plasmolysis (ethyl acetate 1.5%), and enzymatic hydrolysis (Alcalase 0.2%). The efficiency of each process was compared according to soluble solid and protein contents, cell lysis monitoring, and release of intracellular materials, cell viability and microscopic analysis. Results ...
Source: World Journal of Microbiology and Biotechnology - April 24, 2020 Category: Microbiology Source Type: research

Rapid biotransformation of the insensitive munitions compound, 3-nitro-1,2,4-triazol-5-one (NTO), by wastewater sludge
The objective of this study was to explore the ability of wastewater sludges to promote the biotransformation of NTO. Three different sludges, i.e., anaerobic granular sludge, anaerobic digested sludge, and return activated sludge, were used to study the biotransformation of NTO under anaerobic conditions. Three different electron donor amendments were compared- hydrogen, ethanol, and acetate. Mixed microbial communities in each of the three sludge sources were effective in the reductive biotransformation of NTO. 3-amino-1,2,4-triazol-5-one (ATO) was observed as the major product of NTO biotransformation. The highest maxim...
Source: World Journal of Microbiology and Biotechnology - April 23, 2020 Category: Microbiology Source Type: research

Nature and bioprospecting of haloalkaliphilics: a review
AbstractThe haloalkaliphilics are an important subset of extremophiles that grow in salt [upto 33% (wt/vol) NaCl] and alkaline pH (>  9). They are found in hypersaline environments especially in the brines in arid, coastal and deep sea locations, and in alkaline environments, such as soda soils, lakes and deserts. Some authors have described haloalkaliphilic bacteria as moderate halophilic bacteria, but the molecular and classi cal studies revealed that they belong to moderately to extremely halophilic bacteria and archaea. Organic solutes, such as glycine, betaine and other amino acid derivatives, sugars such as...
Source: World Journal of Microbiology and Biotechnology - April 23, 2020 Category: Microbiology Source Type: research

Response of particle-associated bacteria to long-term heavy metal contamination in a tropical estuary
AbstractEstuaries being the connecting link between terrestrial and marine environment, experience spatial variations in the hydrographic variables as well as concentrations of pollutants. The present study reports a contrasting difference in the metal tolerance and enzyme activity of particle-associated bacteria (PAB) isolated from the upstream and downstream reaches of a tropical estuary [Cochin Estuary (CE) in the southwest coast of India], exposed to different levels of heavy metal contamination. The upstream of the estuary has been overloaded with heavy metals in the last few decades, while the downstream is less poll...
Source: World Journal of Microbiology and Biotechnology - April 22, 2020 Category: Microbiology Source Type: research

The promiscuity of Phaseolus vulgaris L. (common bean) for nodulation with rhizobia: a review
AbstractPhaseolus vulgaris L. (common bean) is a legume indigenous to American countries currently cultivated in all continents, which is nodulated by different rhizobial species and symbiovars. Most of species able to nodulate this legume worldwide belong to the genusRhizobium, followed by those belonging to the generaEnsifer (formerlySinorhizobium) andPararhizobium (formerlyRhizobium) and minority by species of the genusBradyrhizobium. All these genera belong to the phylum alpha-Proteobacteria, but the nodulation ofP. vulgaris has also been reported for some species belonging toParaburkholderia andCupriavidus from the be...
Source: World Journal of Microbiology and Biotechnology - April 20, 2020 Category: Microbiology Source Type: research

Transcriptome analysis of Penicillium italicum in response to the flavonoids from Sedum aizoon L.
In this study, RNA sequence analysis was used to investigate the underlying anti-fungal mechanism of flavonoids fromSedum aizoon L. (FSAL) onP. italicum. Significant differences in 3592 genes were observed, including 2507 up-regulated and 1085 down-regulated genes between the FSAL-treated and the control groups. Furthermore, the GO and KEGG analysis results indicated that FSAL inhibited genes related to the integral components of membrane, oxidation –reduction process, mitochondrion, ribosome, and amino acid metabolism. In the presence of FSAL, the cellular constituents, including DNA and RNA were leaked from hyphae ...
Source: World Journal of Microbiology and Biotechnology - April 20, 2020 Category: Microbiology Source Type: research

Dynamics and species diversity of lactic acid bacteria involved in the spontaneous fermentation of various palm tree saps during palm wine tapping in C ôte d’Ivoire
This study is the first to investigate extensively the LAB diversity and dynamics throughout palm trees tapping process in C ôte d’Ivoire and is relevant for future selection of health promoting bacteria. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - April 20, 2020 Category: Microbiology Source Type: research

Biological leaching of rare earth elements
AbstractThe distinctive physico-chemical features of rare earth elements (REEs) have led to an increase in demand by the global market due to their multiple uses in industrial, medical and agricultural implementations. However, the scarcity of REEs and the harsh eco-unfriendly leaching processes from primary sources beside obliviousness  to their recycling from secondary sources, together with the geopolitical situation, have created the need to develop a more sustainable mining strategy. Therefore, there is a growing interest in bio-hydrometallurgy, which may contribute to the scavenging...
Source: World Journal of Microbiology and Biotechnology - April 13, 2020 Category: Microbiology Source Type: research

Incorporation of nonstandard amino acids into proteins: principles and applications
AbstractThe cellular ribosome shows a naturally evolved strong preference for the synthesis of proteins with standard amino acids. An in-depth understanding of the translation process enables scientists to go beyond this natural limitation and engineer translating systems capable of synthesizing proteins with artificially designed and synthesized non-standard amino acids (nsAA) featuring more bulky sidechains. The sidechains can be functional groups, with chosen biophysical or chemical activities, that enable the direct application of these proteins. Alternatively, the sidechains can be designed to contain highly reactive ...
Source: World Journal of Microbiology and Biotechnology - April 8, 2020 Category: Microbiology Source Type: research

Expression of acetaldehyde dehydrogenase ( aldB ) improved ethanol production from xylose by the ethanologenic Escherichia coli RM10
In this study, the role ofaldB gene (encoding for acetaldehyde dehydrogenase, AldB, which oxidizes acetaldehyde to acetic acid) was evaluated for ethanol/acetaldehyde tolerance and xylose fermentation by RM10. Deletion ofaldB gene decreased ethanol tolerance, fermentative cell growth and ethanol production from xylose; while overexpression ofaldB gene improved fermentative cell growth, and increased ethanol production from xylose. The improvement is likely attributed to preventing acetaldehyde accumulation (a toxic intermediate of homoethanol pathway) via AldB catalyzed oxidation. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - March 31, 2020 Category: Microbiology Source Type: research

Interspecific hybridization between cultivated morels Morchella importuna and Morchella sextelata by PEG-induced double inactivated protoplast fusion
AbstractThe commercial production ofMorchella mushrooms calls for urgent breeding of excellent varieties or strains with appropriate tools, such as protoplast fusion. However, the protoplast fusion in morels has not been studied. In this paper, interspecific hybridization between cultivated morels ofM. importuna andM. sextelata by PEG-induced protoplast fusion was conducted. Apart from functional complementation of double inactivated protoplasts, the fusants were characterized by cultural and cultivated characters and molecular markers of random amplified polymorphic DNA (RAPD). The results suggested that the hybrids and t...
Source: World Journal of Microbiology and Biotechnology - March 31, 2020 Category: Microbiology Source Type: research