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MiRNA-92a protects pancreatic B-cell function by targeting KLF2 in diabetes mellitus.
CONCLUSION: Altogether, we verified the function and mechanism of miRNA-92a and provide evidence that miRNA-92a may serve a potential candidate for the clinical treatment for DM. PMID: 29660330 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Wang W, Wang J, Yan M, Jiang J, Bian A Tags: Biochem Biophys Res Commun Source Type: research

Biophysical characterization of actin bundles generated by the Chlamydia trachomatis Tarp effector.
Abstract Chlamydia trachomatis entry into host cells is mediated by pathogen-directed remodeling of the actin cytoskeleton. The chlamydial type III secreted effector, translocated actin recruiting phosphoprotein (Tarp), has been implicated in the recruitment of actin to the site of internalization. Tarp harbors G-actin binding and proline rich domains required for Tarp-mediated actin nucleation as well as unique F-actin binding domains implicated in the formation of actin bundles. Little is known about the mechanical properties of actin bundles generated by Tarp or the mechanism by which Tarp mediates actin bundle...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Ghosh S, Park J, Thomas M, Cruz E, Cardona O, Kang H, Jewett T Tags: Biochem Biophys Res Commun Source Type: research

DUSP14 knockout accelerates cardiac ischemia reperfusion (IR) injury through activating NF- κB and MAPKs signaling pathways modulated by ROS generation.
DUSP14 knockout accelerates cardiac ischemia reperfusion (IR) injury through activating NF-κB and MAPKs signaling pathways modulated by ROS generation. Biochem Biophys Res Commun. 2018 Apr 13;: Authors: Lin B, Xu J, Feng DG, Wang F, Wang JX, Zhao H Abstract Inflammation and oxidative stress are significantly involved in the progression of a variety of diseases, including myocardial ischemia/reperfusion (IR). In the present study, we hypothesized a protective role of dual-specificity phosphatase 14 (DUSP14) in myocardial IR, as well as the underlying molecular mechanism. The results indicated tha...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Lin B, Xu J, Feng DG, Wang F, Wang JX, Zhao H Tags: Biochem Biophys Res Commun Source Type: research

Epitope-based ligation of ICAM-1: Therapeutic target for protection against the development of rheumatoid arthritis.
In this study, we noted that the disease progression, in terms of arthritis score and histopathology of joints, was significantly less severe in the MD-3-treated group than in the vehicle-treated group. Defective production of IL-6 and reduced proliferation of collagen-specific T cells were most remarkable laboratory findings. This type of ligation has a greater advantage over other types of therapeutics, in a sense that simple injection of this antibody inhibits antigen-specific T cell response. Due to the possibility of viral infection in this process, we regularly monitored cytomegalovirus reactivation status without de...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Lee JI, Park HJ, Park HJ, Choi YJ, Kim J, Park JK, Jung KC, Hwang ES, Lee EB, Park SH Tags: Biochem Biophys Res Commun Source Type: research

Fatty acids modulate the efficacy of lutein in cataract prevention: Assessment of oxidative and inflammatory parameters in rats.
Abstract BACKGROUND: Effects of lutein (L) and fatty acids [linoleic acid (LA), eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA) and oleic acid (OA)] on oxidative stress and inflammation in cataract were assessed. METHODS: Cataract was induced in male Wistar rat pups (11 days old) by giving a single dose of sodium selenite (25 μM/kg body weight) by IP. Lutein (1.3 μmol/kg body weight) was given one day before and five days after selenite injection as a micelle with 7.5 mM LA, or 7.5 mM EPA + DHA or 7.5 mM OA. Serum and lens oxidative stress and inflammatory param...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Padmanabha S, Baskaran V Tags: Biochem Biophys Res Commun Source Type: research

mTOR up-regulation of BEX4 promotes lung adenocarcinoma cell proliferation by potentiating OCT4.
Abstract Previously, BEX family members have been reported to participate in cancer development. However, little is known about the role of BEX4 in lung adenocarcinoma (LAC). Here, we found that BEX4 was over-expressed in LAC tissues compared with adjacent tissues. LAC tissues from metastatic patients exhibited higher expression of BEX4 comparing to those from non-metastatic ones. In vitro, BEX4 ectopic expression accelerated the proliferation of both A549 and H1975 cells. By contrast, knockdown of BEX4 suppressed the proliferation of A549 and H1975 cells. BEX4 positively regulated the expression of OCT4, sile...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Zhao Z, Li J, Tan F, Gao S, He J Tags: Biochem Biophys Res Commun Source Type: research

MicroRNA-155 inversely correlates with esophageal cancer progression through regulating tumor-associated macrophage FGF2 expression.
Abstract Esophageal cancer (EC) is one of the most common malignancies with high incidence and mortality. Tumor-associated macrophages (TAMs) in the tumor microenvironment have been linked to the accelerated tumor progression. MicroRNAs (miR) are 19-25 nucleotide-long, noncoding RNA molecules, functioning as modulators of gene expression, and mediate a variety of biological functions, including tumor growth. In the present study, the effects and molecular mechanism of miR-155 in TAMs isolated from EC were explored. The expression of miR-155 and fibroblast growth factor-2 (FGF2) in EC tissues and cell lines were an...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Wang P, Xu LJ, Qin JJ, Zhang L, Zhuang GH Tags: Biochem Biophys Res Commun Source Type: research

Effect of betulinic acid and its ionic derivatives on M-MuLV replication.
In this study, we synthesized more water-soluble ionic derivatives of betulinic acid, and examined their activities against Moloney MuLV (M-MuLV). The mouse fibroblast cells stably infected with M-MuLV, 43D cells, were treated with various doses of betulinic acids and its derivatives, and the viral structural protein Gag in cells and media were detected by western blots. Two ionic derivatives containing the benzalkonium cation were found to inhibit the virus production into media and decreased Gag in cells. However, a cell proliferation assay showed that the benzalkonium cation inhibited the growth of 43D cells, suggesting...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Phillips J, Phillips I, Enya B, Zhao H, Nitta T Tags: Biochem Biophys Res Commun Source Type: research

Different molecular mechanisms of HTLV-1 and HIV LTR activation by TPA.
Abstract HTLV-1 and HIV-1 are retroviruses involved in different human diseases. However, following infection, these viruses inter into a latent state. Tax and Tat are regarded as trans-activators of HTLV-1 and HIV-1 respectively. As it known, during the latent state the infected cells contain low Tax and Tat protein levels, so the activation of these viruses must be independent of these proteins. Here we focus on exploring the mechanism of activation of these viruses by 12-O-tetradecanoylphorbol-13-acetate (TPA), which is a potent activator of protein kinase C (PKC) and considered as a stress-inducing agent. Our ...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Jabareen A, Salman M, Abu-Jaafar A, Huleihel M Tags: Biochem Biophys Res Commun Source Type: research

GLUT5 increases fructose utilization and promotes tumor progression in glioma.
Abstract Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the proliferation and colony formation ability of glioma cells in fructose medium, and found that the abilities of proliferation and colony formation of glioma cells in fructose medium were similar with abilities in glucose medium, however, fructose just partly restored proliferation ability of normal glial cells. To e...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Su C, Li H, Gao W Tags: Biochem Biophys Res Commun Source Type: research

Alteration of global protein SUMOylation in neurons and astrocytes in response to Alzheimer's disease-associated insults.
Abstract SUMOylation, a post-translational modification of lysine residues by small ubiquitin-like modifier (SUMO) proteins, has been implicated in the pathogenesis of neurodegenerative disorders including Alzheimer's disease (AD), and in neuron- and astrocyte-specific physiological functions. Global SUMOylation is increased in the AD mouse brain in the pre-plaque-forming stage but returns to wild-type levels in the plaque-bearing stage. To clarify the reason for the transient change in SUMOylation, we analyzed the alteration of global SUMOylation induced by AD-associated cytotoxic stimuli in neurons and astrocyte...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Maruyama T, Wada H, Abe Y, Niikura T Tags: Biochem Biophys Res Commun Source Type: research

Evaluation of synthetic promoters in Physcomitrella patens.
This study shows that few cis-elements are enough to establish a strong promoter for continuous expression of genes in plants. Along with this study, enhance the number of available promotors to be used in P. patens. It also demonstrate the potential to construct multiple non-native promoters on demand, which would aid to resolve the bottleneck issue of multiple pathway expression in P. patens and other plants. PMID: 29660341 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Peramuna A, Bae H, Rasmussen EK, Dueholm B, Waibel T, Critchley JH, Brzezek K, Roberts M, Simonsen HT Tags: Biochem Biophys Res Commun Source Type: research

Investigation of TRPV1 loss-of-function phenotypes in TRPV1 Leu206Stop mice generated by N-ethyl-N-nitrosourea mutagenesis.
l;der W Abstract N-ethyl-N-nitrosourea (ENU) random mutagenesis was used to generate a mouse model for the analysis of the transient receptor potential vanilloid 1 (TRPV1) cation channel. A transversion from T→A in exon 4 led to a Leu206Stop mutation generating a loss-of-function mutant. The TRPV1 agonist capsaicin was used to analyze functional and nociceptive parameters in vitro and in vivo in TRPV1 Leu206Stop mice and congenic C3HeB/FeJ controls. Capsaicin-induced [Ca2+]i changes in small diameter DRG neurons were significantly diminished in TRPV1 Leu206Stop mice and administration of capsaicin induced nei...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Christoph T, Kögel B, Schiene K, Peters T, Schröder W Tags: Biochem Biophys Res Commun Source Type: research

Transcription of blunt snout bream (Megalobrama amblycephala) HIF3 α and its localization in the nucleus under both normoxic and hypoxic conditions.
Transcription of blunt snout bream (Megalobrama amblycephala) HIF3α and its localization in the nucleus under both normoxic and hypoxic conditions. Biochem Biophys Res Commun. 2018 Apr 13;: Authors: Liu Z, Zhao X, Jiang X, Zou S Abstract Although hypoxia-inducible factor (HIF) 1α and 2α function as master regulators of the transcriptional response to hypoxia, the function of HIF3α and its responses to hypoxic stress remain unclear in teleost fish. Here, we characterized the HIF3α cDNA in hypoxia-sensitive blunt snout bream (Megalobrama amblycephala), with 3059 bp length, ...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Liu Z, Zhao X, Jiang X, Zou S Tags: Biochem Biophys Res Commun Source Type: research

Hyperhomocysteinemia and hyperandrogenemia share PCSK9-LDLR pathway to disrupt lipid homeostasis in PCOS.
Abstract Women with polycystic ovary syndrome (PCOS) are at increased risk of cardiovascular diseases (CVD); however, the independent role of PCOS in the incident CVD remains unknown. There are reports that hyperhomocysteinemia (HHcy), a potential cause of CVD, is frequently associated with PCOS. The present study investigates the independent attributes of hyperandrogenemia (HA), the integral associate of PCOS, and HHcy in causing atherogenic dyslipidemia. Twenty-five-day old rats were treated with homocysteine (Hcy) at 50 mg/kg/day dose level for 12 weeks. The HepG2 cell lines transfected with siRNA directed to...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Mondal K, Chakraborty P, Kabir SN Tags: Biochem Biophys Res Commun Source Type: research

A novel heme oxygenase-1 splice variant, 14kDa HO-1, promotes cell proliferation and increases relative telomere length.
Abstract Alternative splicing is a routine phenomenon which greatly increases the diversity of proteins in eukaryotic cells. In humans, most multi-exonic genes are alternatively spliced and their splice variants confer distinct functions. Heme oxygenase-1 (HO-1, 32 kDa) is an inducible stress responsive protein, which possesses multiple functions in many cellular processes. In the current study, we identified a novel alternative splice isoform of 14 kDa HO-1 generated through exclusion of exon 3, and it is highly expressed in immortalized cells. In contrast to nuclear accumulation of the full-length 32&n...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Bian C, Zhong M, Nisar MF, Wu Y, Ouyang M, Bartsch JW, Zhong JL Tags: Biochem Biophys Res Commun Source Type: research

Wash-free and selective imaging of epithelial cell adhesion molecule (EpCAM) expressing cells with fluorogenic peptide ligands.
Abstract Detection of the cells expressing an epithelial cell adhesion molecule (EpCAM) is a crucial step to identify circulating tumor cells (CTCs) from blood. To detect the EpCAM, we here designed and synthesized a series of fluorogenic peptides. Specifically, we functionalized an EpCAM-binding peptide, Ep114, by replacing its amino acids to an aminophenylalanine that was modified with environmentally sensitive 7-nitro-2,1,3-benzoxadiazole (NBD-amPhe). Among six synthesized peptides, we have found that two peptides, Q4X and V6X (X represents NBD-amPhe), retain the Ep114's binding ability and specifically mark Ep...
Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Tara Bahadur KC, Suga K, Isoshima T, Aigaki T, Ito Y, Shiba K, Uzawa T Tags: Biochem Biophys Res Commun Source Type: research

Interleukin-enhanced binding factor 2 interacts with NLRP3 to inhibit the NLRP3 inflammasome activation.
In this study, by establishing and using a reconstructed NLRP3 inflammasome activation system, we reveal that the NLRP3 inflammasome activation, pro-caspase-1 cleavage, and pro-interleukin-1β (pro-IL-1β) activation are repressed by the interleukin-enhanced binding factor 2 (ILF2). Further studies demonstrate that ILF2 represses the activation of NLRP3 inflammasome through interacting with the NACHT-associated domain (NAD) of NLRP3 and co-localized with NLRP3 in the cytoplasm of HEK293T cells. Finally, by generating a THP-1 cell line stably expressing ILF2 protein using the lentivirus infection system, we demons...
Source: Biochemical and Biophysical Research communications - April 12, 2018 Category: Biochemistry Authors: Jin J, Li A, Wang W, Wu J Tags: Biochem Biophys Res Commun Source Type: research

HDAC11 deletion reduces fructose-induced cardiac dyslipidemia, apoptosis and inflammation by attenuating oxidative stress injury.
Abstract Diabetes mellitus (DM) is a risk factor for abnormal heart development, but the molecular mechanism remains obscure. Histone deacetylase 11 (HDAC11), the most recently identified histone deacetylase, is the sole member of class IV HDACs. However, its role in diabetic cardiac injury is still poorly understood. In the present study, we attempted to explore the effects of HDAC11 on fructose (Fru)-induced cardiac injury using the wild type (HDAC11+/+) and knockout (HDAC11-/-) mice. The results indicated that HDAC11 was significantly expressed in human and mouse diabetic heart failure (DHF) hearts. HDAC11-/- r...
Source: Biochemical and Biophysical Research communications - April 12, 2018 Category: Biochemistry Authors: Fan XD, Wan LL, Duan M, Lu S Tags: Biochem Biophys Res Commun Source Type: research

Overexpression of a repressor MdMYB15L negatively regulates anthocyanin and cold tolerance in red-fleshed callus.
In this study, we identified a bHLH binding motif in the MdMYB15L protein using protein sequence analyses. Yeast two-hybrid and pull-down assays showed that MdMYB15L could interact with MdbHLH33. Overexpressing MdMYB15L in red-fleshed callus inhibited the expression of MdCBF2 and resulted in reduced cold tolerance but did not affect anthocyanin levels. Chip-PCR and EMSA analysis showed that MdMYB15L could bind the type II cis-acting element found in the MdCBF2 promoter. Overexpressing MdMYB15L in red-fleshed callus overexpressing MdbHLH33 also reduced cold tolerance and reduced MdbHLH33-induced anthocyanin biosynthesis. Kn...
Source: Biochemical and Biophysical Research communications - April 12, 2018 Category: Biochemistry Authors: Xu H, Yang G, Zhang J, Wang Y, Zhang T, Wang N, Jiang S, Zhang Z, Chen X Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA UCA1/miR-182/PFKFB2 axis modulates glioblastoma-associated stromal cells-mediated glycolysis and invasion of glioma cells.
In this study, lncRNA UCA1/miR-182 axis has been regarded as a nodal driver of glioma invasion mediated by GB-associated stromal cells (GASCs) and GASC-secreted chemokine CXCL14. In clinical specimens, CXCL14 upregulation in GASCs also correlated with poor prognosis. Notably, CXCL14-high GASCs mediated lncRNA UCA1 upregulation and miR-182 downregulation in glioma cells. Moreover, miR-182 directly bound to the fructose-2,6-biphosphatase PFKFB2; UCA1/miR-182 axis thereby modulated GASC-induced glycolysis in glioma cells. Overall, UCA1/miR-182/PFKFB2 axis modulates chemokine CXCL14 secretion, glycolysis and invasion of glioma...
Source: Biochemical and Biophysical Research communications - April 12, 2018 Category: Biochemistry Authors: He Z, You C, Zhao D Tags: Biochem Biophys Res Commun Source Type: research

Inhibition of NLRP9b attenuates acute lung injury through suppressing inflammation, apoptosis and oxidative stress in murine and cell models.
Abstract Acute lung injury (ALI), known a severe disease along with high morbidity and mortality, is lacking of specific therapies. Inflammation, apoptosis and oxidative stress are critical pathologies that contribute to ALI. Recently, there is study indicated that NLRP9b, a NOD-like receptor (NLR) member, is critical in modulation of inflammatory response. However, the effects of NLRP9b on sepsis-associated ALI, and the underlying molecular mechanism have not been understood. In the present study, the wild type (WT) and NLRP9b-knockout (NLRP9b-/-) mice with C57B/L6 background were subjected to a cecal ligation an...
Source: Biochemical and Biophysical Research communications - April 12, 2018 Category: Biochemistry Authors: Yanling Q, Xiaoning C, Fei B, Liyun F, Huizhong H, Daqing S Tags: Biochem Biophys Res Commun Source Type: research

Role of acid responsive genes in the susceptibility of Escherichia coli to ciclopirox.
Abstract Antibiotic resistance poses a huge threat to the effective treatment of bacterial infections. To circumvent the limitations in developing new antibiotics, researchers are attempting to repurpose pre-developed drugs that are known to be safe. Ciclopirox, an off-patent antifungal agent, inhibits the growth of Gram-negative bacteria, and genes involved in galactose metabolism and lipopolysaccharide (LPS) biosynthesis are plausible antibacterial targets for ciclopirox, since their expression levels partially increase susceptibility at restrictive concentrations. In the present study, to identify new target ge...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Shin J, Cho H, Kim S, Kim KS Tags: Biochem Biophys Res Commun Source Type: research

GSK126 alleviates the obesity phenotype by promoting the differentiation of thermogenic beige adipocytes in diet-induced obese mice.
Abstract A close relationship between epigenetic regulation and obesity has been demonstrated in several recent studies. Histone methyltransferase enhancer of Zeste homolog 2 (Ezh2), which mainly catalyzes trimethylation of histone H3K27 to form H3K27me3 was found to be required for the differentiation of white and brown adipocytes in vitro. Here, we investigated the effects of the Ezh2-specific inhibitor GSK126 in a mouse model of obesity induced by a high-fat diet (HFD). We found that GSK126 treatment reduced body fat, improved glucose tolerance, increased lipolysis and improved cold tolerance in mice by promoti...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Wu X, Wang Y, Wang Y, Wang X, Li J, Chang K, Sun C, Jia Z, Gao S, Wei J, Xu J, Xu Y, Li Q Tags: Biochem Biophys Res Commun Source Type: research

Sphingolipidomics analysis of large clinical cohorts. Part 1: Technical notes and practical considerations.
Abstract Lipids comprise an exceptionally diverse class of bioactive macromolecules. While quantitatively abundant lipid species serve fundamental roles in cell structure and energy metabolism, thousands of structurally-distinct, quantitatively minor species may serve as important regulators of cellular processes. Historically, a complete understanding of the biological roles of these lipids has been limited by a lack of sensitive, discriminating analytical techniques. The class of sphingolipids alone, for example, is known to consist of over 600 different confirmed species, but is likely to include tens of thousa...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Chew WS, Seow WL, Chong JR, Lai MKP, Torta F, Wenk MR, Herr DR Tags: Biochem Biophys Res Commun Source Type: research

Cytoprotective role of ubiquitin against toxicity induced by polyglutamine-expanded aggregates.
In this study, we ectopically expressed polyQ-expanded aggregates (Q103) in MEFs and observed inclusion body formation at the juxtanuclear region, which was independent of cellular Ub levels. In contrast to arsenite treatment, polyQ expression did not affect proteasome function. However, we observed an increased accumulation of Q103 aggregates in Ubc-/- MEFs, which was due to impaired autophagic clearance. Finally, we demonstrated that the increased accumulation of Q103 aggregates under Ub deficiency dramatically reduced the viability of cells. Therefore, our results suggest that the maintenance of proper levels of cellula...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Bae JS, Ryu KY Tags: Biochem Biophys Res Commun Source Type: research

PPM1B depletion in U2OS cells supresses cell growth through RB1-E2F1 pathway and stimulates bleomycin-induced cell death.
Abstract PPM1B is a metal-dependent serine/threonine protein phosphatase, with a similar structure and function to the well-known oncogene in breast cancer, PPM1D (WIP1). However, clinical significance of PPM1B as a pharmacological target in cancer therapy has not been explored. To test if PPM1B can be a drug target in the cellular proliferation and death pathway, the lentiviral PPM1B shRNA was stably expressed in cancer cell lines and its regulatory function in the RB1-E2F1 pathway was examined. We found that PPM1B depletion suppressed cellular proliferation of U2OS cells, accompanied by hyper-phosphorylation of ...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Miller RE, Uwamahoro N, Park JH Tags: Biochem Biophys Res Commun Source Type: research

Sphingolipidomics analysis of large clinical cohorts. Part 2: Potential impact and applications.
Abstract It has been known for decades that the regulation of sphingolipids (SLs) is essential for the proper function of many cellular processes. However, a complete understanding of these processes has been complicated by the structural diversity of these lipids. A well-characterized metabolic pathway is responsible for homeostatic maintenance of hundreds of distinct SL species. This pathway is perturbed in a number of pathological processes, resulting in derangement of the "sphingolipidome." Recently, advances in mass spectrometry (MS) techniques have made it possible to characterize the sphingolipido...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Chong JR, Xiang P, Wang W, Hind T, Chew WS, Ong WY, Lai MKP, Herr DR Tags: Biochem Biophys Res Commun Source Type: research

Regulation of hair follicle development by exosomes derived from dermal papilla cells.
CONCLUSION: DPC-Exos contribute to the regulation of HF growth and development, and provide a potential avenue for the treatment of hair loss. PMID: 29654758 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Zhou L, Wang H, Jing J, Yu L, Wu X, Lu Z Tags: Biochem Biophys Res Commun Source Type: research

Small nucleolar RNA U2_19 promotes hepatocellular carcinoma progression by regulating Wnt/ β-catenin signaling.
Small nucleolar RNA U2_19 promotes hepatocellular carcinoma progression by regulating Wnt/β-catenin signaling. Biochem Biophys Res Commun. 2018 Apr 11;: Authors: Wang H, Ma P, Liu P, Chen B, Liu Z Abstract Emerging evidence suggests that small nucleolar RNAs (snoRNAs) have malfunctioning roles in oncogenesis. In the present study, we investigated the role of box C/D small nucleolar RNA U2_19 (snoU2_19) in the tumorigenesis of hepatocellular carcinoma (HCC). Recently, we screened snoRNAs differential signatures by performing high-throughput small RNA sequence in HCC tissues and validated that upre...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Wang H, Ma P, Liu P, Chen B, Liu Z Tags: Biochem Biophys Res Commun Source Type: research

Melanosome degradation in epidermal keratinocytes related to lysosomal protease cathepsin V.
In this study, we focused on a lysosomal protease cathepsin V (CTSV) to clarify the mechanism underlying melanosome degradation in the keratinocytes. Using immunohistochemical observation, we found that CTSV was highly expressed across the entire epidermis in normal skin; however, CTSV expression levels were lower in the basal layer than those in the stratum corneum side in the hyperpigmented region. Moreover, we found that melanosome degradation was suppressed in CTSV knockdown cells. These results indicated that CTSV is involved in melanosome degradation. PMID: 29654760 [PubMed - as supplied by publisher] (Source: B...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Homma T, Kageyama S, Nishikawa A, Nagata K Tags: Biochem Biophys Res Commun Source Type: research

Particulate matter increases beta-amyloid and activated glial cells in hippocampal tissues of transgenic Alzheimer's mouse: Involvement of PARP-1.
Abstract Exposure to air pollutants, such as particulate matter (PM), has been implicated in neurodegenerative disorders including Alzheimer's disease (AD). However, direct effects of PM on production of β-amyloid (Aβ), a key pathogenic molecule in AD, and its underlying mechanism are still elusive. Given PM's potential to induce oxidative stress in other tissues, we hypothesized that poly(ADP-ribose) polymerase (PARP-1) might be involved in PM-induced neurotoxicity. To address this, we used an ex vivo model of AD, the organotypic hippocampal slice tissue culture from old (12-14 months-of-age) triple tra...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Jang S, Kim EW, Zhang Y, Lee J, Cho SY, Ha J, Kim H, Kim E Tags: Biochem Biophys Res Commun Source Type: research

Egress of murine regulatory T cells from the thymus requires TIPE2.
Abstract Regulatory T cells (Tregs) can be divided into thymus-derived Treg (tTregs) and peripheral induced Tregs (pTregs) in vivo according to their origins and are essential for the maintenance of immune hemostasis and immune tolerance. Tumor necrosis factor-α-induced protein 8 like 2 (TIPE2) is expressed primarily by immune cells and is a negative regulator of the innate and adaptive immune response. Previous studies indicate that TIPE2 is required for the expression of Treg signature genes and promotes leading-edge formation in neutrophils through cytoskeleton remodeling. In the current study, we showed ...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Fan T, Huang X, Liu C, Liu R, Wang T, Ruan Q Tags: Biochem Biophys Res Commun Source Type: research

ST6GAL1 negatively regulates monocyte transendothelial migration and atherosclerosis development.
In this study, we demonstrated that the expression level of ST6GAL1 in vascular endothelium was significantly decreased in atherosclerosis development process, while obviously recovered after atherosclerosis regression. Further analysis showed that knockdown of ST6GAL1 by RNA interference in vascular endothelial EA. hy926 obviously promoted TNFα-triggered monocyte-transendothelial migration, whereas overexpression of ST6GAL1 strongly inhibited monocyte-transendothelial migration. Moreover, we firstly found β-catenin is a sialylated protein and its sialylation level is decreased in TNFα-treated EA. hy926 ...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Zhang J, Liu Y, Deng X, Chen L, Yang X, Yu C Tags: Biochem Biophys Res Commun Source Type: research

Rg1 inhibits high glucose-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway.
Abstract Recent study has showed that Ginsenoside Rg1, the mian active compound of Panax ginseng, could ameliorate oxidative stress and myocardial apoptosis in diabetes mellitus. However, the roles and mechanisms of Rg1 in proliferative diabetic retinopathy (PDR) are still unclear. In the present study, we aimed to investigate the effects of Rg1 on mesenchymal activation of high-glucose (HG) cultured müller cells. High glucose conditions up-regulate MMP-2, MMP-9 and down-regulate TIMP-2, and promote mesenchymal activation in Müller cells. And Rg1 inhibits the HG-induced mesenchymal activation and HG-incr...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Xue LP, Fu XL, Hu M, Zhang LW, Li YD, Peng YL, Ding P Tags: Biochem Biophys Res Commun Source Type: research

Adipose mesenchymal stem cell-derived exosomes stimulated by hydrogen peroxide enhanced skin flap recovery in ischemia-reperfusion injury.
Abstract BACKGROUND: Mesenchymal stem cell (MSC)-derived exosomes have been recognized as new candidates for the treatment of ischemic disease or injury and may be an alternative treatment for cell therapy. This aim of the study was to evaluate whether exosomes derived from adipose mesenchymal stem cell (ADSC) can protect the skin flap during ischemia-reperfusion (I/R) injury and induce neovascularization. METHODS: To investigate the effects of exosomes in the I/R injury of flap transplantation in vivo, flaps were subjected to 6 h of ischemia by ligating the left superficial inferior epigastric vessels (SIE...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Bai Y, Han YD, Yan XL, Ren J, Zeng Q, Li XD, Pei XT, Han Y Tags: Biochem Biophys Res Commun Source Type: research

Orai1 downregulation impairs lymphocyte function in type 2 diabetes mellitus.
CONCLUSION: This study demonstrates that the declined Orai1 expression, at least partly, contributes to the downregulated Ca2+ entry during lymphocyte excitation, providing an important mechanism for T lymphocyte malfunction in diabetes. PMID: 29654766 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Wang H, Wang C, Wang L, Liu T, Wang Z, You H, Zheng Y, Luo D Tags: Biochem Biophys Res Commun Source Type: research

A novel small RNA S042 increases acid tolerance in Lactococcus lactis F44.
This study served to highlight the role of the novel sRNA S042 in acid resistance of L. lactis and provided new insights into the response mechanism of acid stress. PMID: 29654767 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Wu H, Song S, Tian K, Zhou D, Wang B, Liu J, Zhu H, Qiao J Tags: Biochem Biophys Res Commun Source Type: research

Seed-specific overexpression of AtFAX1 increases seed oil content in Arabidopsis.
In this study, we overexpressed AtFAX1 under the control of a seed specific promoter in Arabidopsis. The resultant overexpression lines (OEs) produced seeds which contained 21-33% more oil and 24-30% more protein per seed than those of the wild type (WT). The increased oil content was probably because of the enhanced FA and TAG synthetic activity. The seed size and weight were both increased accordingly. In addition, the seed number per silique and silique number per plant had no changes in transgenic plants. Taken together, our results demonstrated that seed specific overexpression of AtFAX1 could promote oil accumulation...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Tian Y, Lv X, Xie G, Zhang J, Xu Y, Chen F Tags: Biochem Biophys Res Commun Source Type: research

Apixaban attenuates ischemia-induced myocardial fibrosis by inhibition of Gq/PKC signaling.
Abstract It was previously found that patients with symptom of myocardial dysfunction had increased levels of thrombin. Apixaban is one of the novel oral anticoagulant drugs widely used in clinic. As the inhibitor of FXa (prothrombin), it inhibits prothrombin conversion into thrombin leading to thrombin deficiency in vivo. However, the effects of apixaban on myocardial fibrosis were still unclear, and the concomitant molecular mechanisms remain to be investigated. Here, we showed that myocardial fibrosis-bearing mice induced by continuous myocardial ischemia (MI) had higher levels of thrombin. Orally administratio...
Source: Biochemical and Biophysical Research communications - April 11, 2018 Category: Biochemistry Authors: Shi G, Yang X, Pan M, Sun J, Ke H, Zhang C, Geng H Tags: Biochem Biophys Res Commun Source Type: research

β1-integrin is a cell-autonomous factor mediating the Numb pathway for cardiac progenitor maintenance.
β1-integrin is a cell-autonomous factor mediating the Numb pathway for cardiac progenitor maintenance. Biochem Biophys Res Commun. 2018 Apr 10;: Authors: Gibbs B, Shenje L, Andersen P, Miyamoto M, Kwon C Abstract Proper control of multipotent/stem cell number and fate is essential for ensuing organ formation during development. β1-integrin, a subfamily of cell surface receptors, has a conserved role in maintenance of multipotent/stem cells, including renal progenitor cells, follicle stem cells, epidermal stem cells and neural stem cells. However, it remains unclear whether β1-integrin h...
Source: Biochemical and Biophysical Research communications - April 10, 2018 Category: Biochemistry Authors: Gibbs B, Shenje L, Andersen P, Miyamoto M, Kwon C Tags: Biochem Biophys Res Commun Source Type: research

Visceral adipose tissue-derived serine protease inhibitor accelerates cholesterol efflux by up-regulating ABCA1 expression via the NF- κB/miR-33a pathway in THP-1 macropahge-derived foam cells.
Visceral adipose tissue-derived serine protease inhibitor accelerates cholesterol efflux by up-regulating ABCA1 expression via the NF-κB/miR-33a pathway in THP-1 macropahge-derived foam cells. Biochem Biophys Res Commun. 2018 Apr 10;: Authors: Gao JH, Zeng MY, Yu XH, Zeng GF, He LH, Zheng XL, Zhang DW, Ouyang XP, Tang CK Abstract Atherosclerosis is a dyslipidemia disease characterized by foam cell formation driven by the accumulation of lipids. Visceral adipose tissue-derived serine protease inhibitor (vaspin) is known to suppress the development of atherosclerosis via its anti-inflammatory prop...
Source: Biochemical and Biophysical Research communications - April 10, 2018 Category: Biochemistry Authors: Gao JH, Zeng MY, Yu XH, Zeng GF, He LH, Zheng XL, Zhang DW, Ouyang XP, Tang CK Tags: Biochem Biophys Res Commun Source Type: research

Sterol regulatory element binding protein 1 trans-activates 25-hydroxy vitamin D3 24-hydroxylase gene expression in renal proximal tubular cells.
Abstract The physiological activity of the steroid derived hormone vitamin D is regulated by several enzymatic steps. Both 25-hydroxy vitamin D3 1α-hydroxylase (CYP27B1) and 25-hydroxyvitamin D3 24-hydroxylase (CYP24A1) modulate blood levels of 1,25-dihydroxyvitamin D3, an activated form of vitamin D. We previously demonstrated that CYP27B1 expression was trans-activated by sterol regulatory element binding protein 1 (SREBP1), although whether SREBP1 also regulates CYP24A1 transcription was unclear. Here we investigated the ability of SREBP1 to affect CYP24A1 transcription. In a luciferase reporter assay, mo...
Source: Biochemical and Biophysical Research communications - April 10, 2018 Category: Biochemistry Authors: Kagawa T, Kozai M, Masuda M, Harada N, Nakahashi O, Tajiri M, Yoshikawa R, Nakao M, Takei Y, Iwano M, Takeda E, Taketani Y, Yamamoto H Tags: Biochem Biophys Res Commun Source Type: research

The malaria parasite RhopH protein complex interacts with erythrocyte calmyrin identified from a comprehensive erythrocyte protein library.
Abstract Malaria merozoite apical organelles; microneme and rhoptry secreted proteins play functional roles during and following invasion of host erythrocytes. Among numerous proteins, the rhoptries discharge high molecular weight proteins known as RhopH complex. Recent reports suggest that the RhopH complex is essential for growth and survival of the malaria parasite within erythrocytes. However, an in-depth understanding of the host-parasite molecular interactions is indispensable. Here we utilized a comprehensive mouse erythrocyte protein library consisting of 443 proteins produced by a wheat germ cell-free sys...
Source: Biochemical and Biophysical Research communications - April 10, 2018 Category: Biochemistry Authors: Miura T, Takeo S, Ntege EH, Otsuki H, Sawasaki T, Ishino T, Takashima E, Tsuboi T Tags: Biochem Biophys Res Commun Source Type: research

CST3 and GDF15 ameliorate renal fibrosis by inhibiting fibroblast growth and activation.
Abstract The final strategies to care patients with end-stage renal fibrosis rely on dialysis and kidney transplantation. Because such treatments are invasive and cause health problems eventually, it is necessary to develop new therapeutic strategies for delaying the disease progress. We here searched for cytokines showing an anti-fibrotic activity in cell-based experiments. Cystatin C (CST3) and Growth differentiation factor 15 (GDF15) were identified to have anti-fibrotic activities in a cytokine array screening. In primary fibroblasts isolated from the mouse kidneys subjected to ureteral obstruction-induced fib...
Source: Biochemical and Biophysical Research communications - April 10, 2018 Category: Biochemistry Authors: Kim YI, Shin HW, Chun YS, Park JW Tags: Biochem Biophys Res Commun Source Type: research

H2A.Z-containing nucleosomes are evicted to activate AtMYB44 transcription in response to salt stress.
Abstract Transcripts of the Arabidopsis transcription factor gene, AtMYB44, accumulate rapidly to mediate a tolerance mechanism in response to salt stress. The AtMYB44 promoter is activated by salt stress, as illustrated in AtMYB44pro::GUS transgenic plants. Chromatin immunoprecipitation (ChIP) assays revealed that RNA polymerases were enriched on the AtMYB44 gene, especially on TSS-proximal regions, and nucleosome density was markedly reduced in the AtMYB44 gene-body region in response to salt stress. In addition, H2A.Z occupation was significantly decreased at the AtMYB44 promoter, transcription start site (TSS)...
Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Nguyen NH, Cheong JJ Tags: Biochem Biophys Res Commun Source Type: research

Involvement of prostatic interstitial cells of Cajal in inflammatory cytokines-elicited catecholamines production: Implications for the pathophysiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).
Abstract In a previous work using guinea pig prostate, we have identified a novel interstitial cells of Cajal (ICCs) which possess close contacts between sympathetic nerve bundles and smooth muscle cells. The ability of prostatic ICCs in mediating excitatory neural inputs was therefore studied using isolated murine prostate ICCs by collagenase digestion combined with FACS method. RT-PCR and Western blotting analyses revealed that prostatic ICCs under a quiescent state expressed abundantly the rate-limiting enzymes essential for catecholamine synthesis. Moreover, distinct proinflammatory cytokines (e.g. IL-1β,...
Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Wang J, Zhang B, Jiao Y, Xu Z, Qian B, Wang Q Tags: Biochem Biophys Res Commun Source Type: research

Autophagy alteration prevents primary cilium disassembly in RPE1 cells.
In this study, we found that the gradual abatement in autophagy during serum-restimulation was a dynamic process and significantly correlated with the disassembly of primary cilium in human retinal pigmented epithelial (RPE1) cells. Although autophagy activity was gradually decreased during serum-restimulation, the alteration in autophagy under the same condition prevented the disassembly of the primary cilium. Autophagy inhibitors such as chloroquine, U18666A and 3-methyladenine (3-MA) retained both the number of ciliated cells and cilium length. In contrast, rapamycin treatment during serum-restimulation maintained the n...
Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Maharjan Y, Lee JN, Kwak S, Lim H, Dutta RK, Liu ZQ, So HS, Park R Tags: Biochem Biophys Res Commun Source Type: research

A spherical aberration-free microscopy system for live brain imaging.
Abstract The high-resolution in vivo imaging of mouse brain for quantitative analysis of fine structures, such as dendritic spines, requires objectives with high numerical apertures (NAs) and long working distances (WDs). However, this imaging approach is often hampered by spherical aberration (SA) that results from the mismatch of refractive indices in the optical path and becomes more severe with increasing depth of target from the brain surface. Whereas a revolving objective correction collar has been designed to compensate SA, its adjustment requires manual operation and is inevitably accompanied by considerab...
Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Ue Y, Monai H, Higuchi K, Nishiwaki D, Tajima T, Okazaki K, Hama H, Hirase H, Miyawaki A Tags: Biochem Biophys Res Commun Source Type: research

High glucose downregulates the effects of autophagy on osteoclastogenesis via the AMPK/mTOR/ULK1 pathway.
Abstract Diabetes is a chronic disease that disrupts the balance between bone formation and bone desorption, which can lead to osteoporosis, increasing the risk of fracture. However, compared with osteoblasts, the biological effects of hyperglycemia on osteoclastogenesis remain to be elucidated. Therefore, we investigated the impact of glucose at different concentrations (5.5, 10.5, 15.5, 20.5, 25.5, and 30.5 mM) on osteoclastogenesis using RAW264.7 cells. Cell proliferation was measured with the cell counting kit-8 assay, and osteoclastogenesis was detected with tartrate-resistant acid phosphatase staining an...
Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Cai ZY, Yang B, Shi YX, Zhang WL, Liu F, Zhao W, Yang MW Tags: Biochem Biophys Res Commun Source Type: research