Determinants of lentiviral Vpx-CRL4 E3 ligase-mediated SAMHD1 degradation in the substrate adaptor protein DCAF1.
Abstract The lentiviral accessory protein Vpx enhances viral replication in macrophages, dendritic cells and resting CD4+ T cells by utilizing the host CRL4-DCAF1 E3 ligase to trigger the degradation of the intrinsic antiviral factor SAMHD1. Distinct from the species-specific recognition of either the N or C-terminus of SAMHD1 by Vpx proteins of different HIV-2 and SIV lineages, Vpx recruits SAMHD1 onto the same CRL4-DCAF1 complex. However, the determinants in DCAF1 that are required for Vpx-mediated SAMHD1 degradation have not been well characterized. Here, we demonstrate that the viral protein Vpx is resistant t...
Source: Biochemical and Biophysical Research communications - April 17, 2019 Category: Biochemistry Authors: Guo H, Zhang N, Shen S, Yu XF, Wei W Tags: Biochem Biophys Res Commun Source Type: research

Corrigendum to 'Long non-coding RNA Linc00320 inhibits glioma cell proliferation through restraining Wnt/ β-catenin signaling', Biochem Biophys Res Commun. 2019 Jan 8; 508(2):458-464.
Corrigendum to 'Long non-coding RNA Linc00320 inhibits glioma cell proliferation through restraining Wnt/β-catenin signaling', Biochem Biophys Res Commun. 2019 Jan 8; 508(2):458-464. Biochem Biophys Res Commun. 2019 Apr 17;: Authors: Tian S, Liu W, Pan Y, Zhan S PMID: 31003778 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 17, 2019 Category: Biochemistry Authors: Tian S, Liu W, Pan Y, Zhan S Tags: Biochem Biophys Res Commun Source Type: research

Inhibition of GIP signaling extends lifespan without caloric restriction.
CONCLUSIONS: Although maintenance of CR is difficult, food intake and muscle endurance of Gipr-/- mice were similar to those of wild-type mice. Inhibition of GIP signaling may be a novel strategy to extend the lifespan of diabetic patients. PMID: 31003779 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 17, 2019 Category: Biochemistry Authors: Hoizumi M, Sato T, Shimizu T, Kato S, Tsukiyama K, Narita T, Fujita H, Morii T, Sassa MH, Seino Y, Yamada Y Tags: Biochem Biophys Res Commun Source Type: research

Arsenic trioxide inhibits EMT in hepatocellular carcinoma by promoting lncRNA MEG3 via PKM2.
In conclusion, our study demonstrates a novel mechanism by which arsenic trioxide inhibits EMT in hepatocellular carcinoma by promoting lncRNA MEG3 and PKM2 negatively regulating MEG3. PMID: 31003765 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Fan Z, He J, Fu T, Zhang W, Yang G, Qu X, Liu R, Lv L, Wang J Tags: Biochem Biophys Res Commun Source Type: research

Exosomes derived from plasma of septic patients inhibit apoptosis of T  lymphocytes by down-regulating bad via hsa-miR-7-5p.
In conclusion, we provide evidence that Sepsis-Exos participate in ameliorating apoptosis of T lymphocytes by directly suppressing Bad via hsa-miR-7-5p. PMID: 31003766 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Deng JN, Li YQ, Liu Y, Li Q, Hu Y, Xu JQ, Sun TY, Xie LX Tags: Biochem Biophys Res Commun Source Type: research

Effects of epigallocatechin-3-gallate on the HMGB1/RAGE pathway in PM2.5-exposed asthmatic rats.
In conclusion, our results suggest that HMGB1 and RAGE are involved in the pathogenesis of asthma. PM2.5 exposure significantly aggravated airway inflammation injury in asthmatic rats. EGCG can reduce lung injury and airway remodeling in PM2.5-exposed asthmatic rats and has lung protective effects. The mechanism may be related to regulation of the HMGB1/RAGE signaling pathway. Our results may provide new ideas and methods for the prevention and treatment of PM2.5-induced asthma. PMID: 31003767 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Li Y, Chen L, Guo F, Cao Y, Hu W, Shi Y, Lin X, Hou J, Li L, Ding X, Guo Y Tags: Biochem Biophys Res Commun Source Type: research

Process of immunogenic cell death caused by disulfiram as the anti-colorectal cancer candidate.
CONCLUSION: In conclusion, our results show that DSF/Cu exerts anti-colorectal cancer and its underlying mechanisms are associated with the enhancement of molecules expression of cell ICD. These results provide experimental evidence and theory basis of therapy for developing the DSF/Cu as the drug for CRC. PMID: 31003768 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: You SY, Rui W, Chen ST, Chen HC, Liu XW, Huang J, Chen HY Tags: Biochem Biophys Res Commun Source Type: research

MicroRNA-146b-5p protects oligodendrocyte precursor cells from oxygen/glucose deprivation-induced injury through regulating Keap1/Nrf2 signaling via targeting bromodomain-containing protein 4.
In this study, we aimed to investigate the potential function of miR-146b-45p in regulating oxygen/glucose deprivation (OGD)-induced injury of OPCs and explore the underlying mechanism. Herein, we found that miR-146b-5p expression was reduced in OPCs exposed to OGD. Functional experiments showed that miR-146b-5p overexpression promoted cell growth and viability, and reduced the apoptosis and oxidative stress in OGD-injured OPCs, while miR-146b-5p inhibition showed an opposite effect. Interestingly, bromodomain-containing protein 4 (Brd4) was identified as a target gene of miR-146b-5p. Brd4 expression was negatively modulat...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Li X, Zhang W, Xiao M, Wang F, Zhou P, Yang J, Chen X Tags: Biochem Biophys Res Commun Source Type: research

Microglia promote the proliferation of neural precursor cells by secreting osteopontin.
Abstract Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation. Microglia surrounding the subventricular zone express osteopontin (OPN) during brain development. The present study investigated the role of microglia in proliferation of NPCs in vitro, and identified the OPN receptor critical for proliferation of NPCs. Microglia co-cultured with NPCs in the presence of an OPN-neutralizi...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Yamamiya M, Tanabe S, Muramatsu R Tags: Biochem Biophys Res Commun Source Type: research

Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII.
; J Abstract Blood coagulation factor VIII (FVIII) can bind calcium ions and ion-protein interactions appear central importance for both their structure and function in coagulation cascade. However, the mechanism and details of how calcium dependent structure change of proteins to fulfill their function remain to be fully defined. In this work, PeakForce Quantitative Nanomechanics (PF-QNM) mode atomic force microscopy (AFM) was used to map the topography and mechanical properties of FVIII with single protein resolution under different calcium concentrations. The obtained nanomechanical spectroscopy showed that cal...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Cheng J, Wang Y, Pan Y, Li X, Hu J, Lü J Tags: Biochem Biophys Res Commun Source Type: research

Construction of the common cortical space by spontaneous activity and its application in the mouse cortex.
Abstract Wide-field optical imaging of the animal brain is a useful technique for measuring brain dynamics, including spatial structure. However, quantitative inter-animal comparison is difficult due to lack of the common cortical space that can normalize individually imaged brains as done in human functional MRI studies. Here, by using wide-field functional Ca2+ imaging on anesthetized transgenic mice expressing G-CaMP7 in astrocytes and excitatory neutrons, we attempted to establish the common cortical space in mice, which can be useful as a standard of functional brain map. We initially reconstructed cortical a...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: O'Hashi K, Sohya K, Matsuno H, Tsuchimine S, Kunugi H Tags: Biochem Biophys Res Commun Source Type: research

Tetrandrine induces apoptosis in human neuroblastoma through regulating the Hippo/YAP signaling pathway.
In this study, we confirmed that TET inhibits proliferation and induces apoptosis in neuroblastoma (NB) in vitro and in vivo. Moreover, we revealed that the anti-cancer ability of TET is associated with a decreased expression of anti-apoptotic Bcl-2. Importantly, we demonstrated that the Hippo/YAP pathway is involved in down-regulating of Bcl-2. Notably, YAP overexpression promoted proliferation and suppressed apoptosis, even partially reversed TET-induced effects in NB cells. Our findings support the prospect that TET could be a potential therapeutic agent for NB, and suggest that targeting the Hippo/YAP pathway...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Zhao Q, Jia X, Zhang Y, Dong Y, Lei Y, Tan X, Williamson RA, Wang A, Zhang D, Ma J Tags: Biochem Biophys Res Commun Source Type: research

NIPA2 regulates osteoblast function via its effect on apoptosis pathways in type 2 diabetes osteoporosis.
In this study we aimed to investigate the specific role and mechanism of NIPA2 in the pathogenesis of type 2 diabetes osteoporosis. We first used western blotting, PCR, immunofluorescence, and magnesium ion probes to detect changes of NIPA2 and intracellular magnesium levels in osteoblasts at different concentrations of advanced glycation end products (AGEs). We then up- or down-regulated NIPA2 using a lentivirus and analyzed apoptotic biomarkers as well as the osteogenic ability of osteoblasts. We found that AGEs dose-dependently down-regulated the expression of NIPA2 in osteoblasts. NIPA2 also regulated osteoblast apopto...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Zhao W, Zhang WL, Yang B, Sun J, Yang MW Tags: Biochem Biophys Res Commun Source Type: research

Prenylated Rab acceptor RABAC1 inhibits anti-apoptotic protein BCL2A1 and induces apoptosis.
Abstract The B cell lymphoma 2 (BCL2) family of proteins constitutes a critical intracellular checkpoint in the intrinsic apoptosis pathway. Among BCL2 members, the anti-apoptotic protein BCL2A1 mediates the resistance to BCL2 inhibitors and may be considered as a target for anti-cancer therapy. Here, we report that prenylated Rab acceptor 1 (RABAC1 or PRA1) inhibits the anti-apoptotic activity of BCL2A1 and induces apoptosis in AGS gastric cancer cells. Protein interaction of BCL2A1 and RABAC1 was verified by an in-vitro glutathione-S-transferase pull-down assay, immunoprecipitation, and confocal microscopy. When...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Kim JT, Cho HJ, Cho MY, Lim J, Park ES, Lim JS, Lee HG Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA LOXL1-AS1 acts as a ceRNA for miR-324-3p to  contribute to cholangiocarcinoma progression via modulation of ATP-binding cassette transporter A1.
Long non-coding RNA LOXL1-AS1 acts as a ceRNA for miR-324-3p to contribute to cholangiocarcinoma progression via modulation of ATP-binding cassette transporter A1. Biochem Biophys Res Commun. 2019 Apr 16;: Authors: Zhang B, Zhou M, Zou L, Miao J, Wang Y, Li Y, Lu S, Yu J Abstract Accumulating evidence has appreciated long non-coding RNAs (lncRNAs) as novel prognostic markers and therapeutic targets in malignant carcinomas. Here, we aim to investigate the value of a novel cancer-related lncRNA, LOXL1-AS1, in cholangiocarcinoma (CCA). LOXL1-AS1 was found overexpressed in CCA tissues screened by hig...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Zhang B, Zhou M, Zou L, Miao J, Wang Y, Li Y, Lu S, Yu J Tags: Biochem Biophys Res Commun Source Type: research

Ribavirin sensitizes nasopharyngeal carcinoma to 5-fluorouracil through suppressing 5-fluorouracil-induced ERK-dependent-eIF4E activation.
Abstract Although overexpression of eukaryotic translation initiation factor 4E (eIF4E) is associated with enhanced growth and poor prognosis in nasopharyngeal carcinoma (NPC), the function of eIF4E in NPC response to chemotherapy has not been revealed. In this work, we demonstrate that eIF4E inhibition using both ribavirin and siRNA targets NPC cells and enhances the efficacy of 5-fluorouracil (5-FU). Mechanism studies indicate that 5-FU treatment increases phosphorylation of eIF4E in NPC cells, and this is dependent on ERK activation. eIF4E inhibition thus significantly sensitizes NPC cell response to 5-FU. Of n...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Hu Z, Zhen L, Li Q, Han Q, Hua Q Tags: Biochem Biophys Res Commun Source Type: research

Sestrin 2 protects against metabolic stress in a p53-independent manner.
Abstract Glucose limitation activates p53, which functions as an adaptive response to maintain cell survival. However, p53 is frequently deleted or mutated in a variety of tumors, while most cancer cells can acclimatize themselves to metabolically unfavorable surrounding, indicating that alternative mechanisms other than p53 transactivation underly adaptive response of cancer cells with p53 deletion or mutation to metabolically hostile environment. Sestrin 2 (SESN2) is a p53 downstream target, which plays a protective role against various stressful stimuli, such as genotoxic, energetic, and oxidative stress. In th...
Source: Biochemical and Biophysical Research communications - April 16, 2019 Category: Biochemistry Authors: Wang JM, Liu BQ, Li C, Du ZX, Sun J, Yan J, Jiang JY, Wang HQ Tags: Biochem Biophys Res Commun Source Type: research

A novel circular RNA circ-ZNF652 promotes hepatocellular carcinoma metastasis through inducing snail-mediated epithelial-mesenchymal transition by sponging miR-203/miR-502-5p.
Abstract Circular RNA (circRNA), a special class of non-coding RNA, is increasingly being realized as a critical regulator in human diseases, including carcinomas. However, its role in hepatocellular carcinoma (HCC) metastasis remains largely unknown. Herein, we enrolled three Gene Expression Omnibus (GEO) databases and screened and identified a novel circRNA, circ-ZNF652 (hsa_circ_0003258), which was significantly upregulated in HCC tissues and cell lines. Importantly, HCC patients with high circ-ZNF652 expression were more prone to vascular invasion, intrahepatic metastasis, distant metastasis, and poor outcome....
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Guo J, Duan H, Li Y, Yang L, Yuan L Tags: Biochem Biophys Res Commun Source Type: research

microRNA-4500 inhibits human glioma cell progression by targeting IGF2BP1.
Abstract Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) overexpression promotes glioma cell progression. The aim of the current study is to silence IGF2BP1 in glioma cells by the microRNA (miRNA) strategy. The bio-informatic analyses identified that microRNA-4500 (miR-4500) putatively targets 3'-UTR (3'-untranslated region) of IGF2BP1. In A172 cells and primary human glioma cells ectopic overexpression of the wild-type miR-4500 (but not the mutant form) downregulated IGF2BP1 and its target genes (Gli1, IGF2 and c-Myc). Functional studies show that ectopic miR-4500 overexpression inhibited glioma c...
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Li ZW, Xue M, Zhu BX, Yue CL, Chen M, Qin HH Tags: Biochem Biophys Res Commun Source Type: research

Interleukin-6 is required for Neuregulin-1 induced HER2 signaling in lung epithelium.
Abstract A clear understanding of the mechanisms that regulate the alveolar epithelium's barrier is critical to develop new therapeutic strategies to mitigate lung injury. The HER2/HER3 receptor tyrosine kinase complex plays a central role in maintaining the alveolar-capillary barrier. This receptor complex is activated by its ligand, neuregulin-1 (NRG-1). Interleukin-6 (IL-6) is also known to induce HER2 signaling through HER2 transphosphorylation by the IL-6 receptor (IL-6R) complex (1). Due to this interaction, we hypothesized that NRG-1 and IL-6 cooperatively interacted to activate the HER2/HER3 complex. Studi...
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Mishra R, Foster DG, Finigan JH, Kern JA Tags: Biochem Biophys Res Commun Source Type: research

IGF2BP1 promotes LPS-induced NF κB activation and pro-inflammatory cytokines production in human macrophages and monocytes.
CONCLUSION: IGF2BP1 promotes LPS-induced NFκB signalling and transcriptional activation in human macrophages and monocytes. PMID: 31000200 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Xie J, Li Q, Zhu XH, Gao Y, Zhao WH Tags: Biochem Biophys Res Commun Source Type: research

Diastereomers of a mono-substituted phosphoryl guanidine trideoxyribonucleotide: Isolation and properties.
Abstract Recently, a new type of nucleic acid analogues with modified phosphate group, namely, phosphoryl guanidine oligonucleotides, has been described. In the present work, we assess the difference between diastereomers of a mono-substituted phosphoryl guanidine oligonucleotide and analyze their resistance to nuclease digestion. Individual diastereomers ('fast' and 'slow') of a trideoxynucleotide d (TpCp*A) were isolated by reverse-phase HPLC. Snake venom phosphodiesterase digestion showed that the native trideoxynucleotide was fully degraded after 30 min, whereas both 'fast' and 'slow' diastereomers of d (...
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Lomzov AA, Kupryushkin MS, Shernyukov AV, Nekrasov MD, Dovydenko IS, Stetsenko DA, Pyshnyi DV Tags: Biochem Biophys Res Commun Source Type: research

Dysregulation of humoral immunity in Foxp3 conditional-knockout mice.
In this study, we developed Foxp3 conditional-knockout mice (Foxp3floxR26CreERT2) in which the Foxp3 gene was inducibly deleted by tamoxifen administration. After oral administration of tamoxifen, titers of immunoglobulins, particularly IgG2c and IgE, were increased in Foxp3floxR26CreERT2 mice compared with that in controls. Under these conditions, CD4+ T cells from Foxp3floxR26CreERT2 mice had increased expression of several activation markers, including inducible costimulator and CD40 ligand, as well as the cytokines interleukin 4 and interferon gamma. In addition, the proportions of T follicular helper (Tfh) cells and g...
Source: Biochemical and Biophysical Research communications - April 15, 2019 Category: Biochemistry Authors: Tai Y, Sakamoto K, Takano A, Haga K, Harada Y Tags: Biochem Biophys Res Commun Source Type: research

Construction of the safe neutralizing assay system using pseudotyped Nipah virus and G protein-specific monoclonal antibody.
Abstract Nipah virus (NiV) is a recently emerged paramyxovirus that causes acute respiratory illness and fatal encephalitis in a broad spectrum of vertebrates, including humans. Due to its high pathogenicity and mortality rates, NiV requires handling in biosafety level-4 (BSL-4) containment facilities and no effective vaccines or therapeutic agents are currently available. Since current diagnostic tests for detecting serum neutralizing antibodies against NiV mainly employ live viruses, establishment of more safe and robust alternative diagnostic methods is an essential medical requirement. Here, we have developed ...
Source: Biochemical and Biophysical Research communications - April 14, 2019 Category: Biochemistry Authors: Bae SE, Kim SS, Moon ST, Cho YD, Lee H, Lee JY, Shin HY, Lee HJ, Kim YB Tags: Biochem Biophys Res Commun Source Type: research

Structural and mechanistic insights into modified G-quadruplex thrombin-binding DNA aptamers.
Abstract Thrombin-binding aptamer (TBA) can fold into a G-quadruplex structure necessary for interacting with thrombin. When one thymidine residue of the TGT loop at position 7 is replaced with unlocked uracil (UNA), d-isothymidine (D-isoT) or l-isothymidine (L-isoT), these modified sequences display different activities. To date, the mechanisms of how D/L-isoT and UNA influence the biological properties of TBA have not been illustrated in the literature. In this paper, we fill this gap by probing the structure variations and binding modes of these modified TBAs via molecular dynamics (MD) simulation and free ener...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Sun L, Xie X, Weng W, Jin H Tags: Biochem Biophys Res Commun Source Type: research

Subpollens delivery of Platanus acerifolia pollen allergen Pla a3 and nucleic acid into lungs and cells.
This study focused on the release, transport and characteristic of Pla a3 allergen of the Platanus acerifolia pollen. Pla a3 protein was purified by prokaryotic expression system for preparation of polyclonal antibody. The distribution and release of Pla a3 protein in pollen were observed by immunohistochemistry. Mice were immunized with purified Pla a3 protein and SPPs, respectively. The pathological examination of mouse lung tissue proved that SPPs, as a fine particle in the range of 0.1-1μm, can enter the deep part of the lung directly through the respiratory tract and led to inflammation. Furthermore, DAPI staining ...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Fucheng Y, Zheng Z, Yichun L, Yang L, Luying Z, Jiaxian P, Wei Z, Shumin Z, Senlin L Tags: Biochem Biophys Res Commun Source Type: research

Hd3a and OsFD1 negatively regulate rice resistance to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola.
Abstract In rice, Hd3a, GF14 and OsFD1 proteins, forming florigen activation complex, are key components in flowering time regulation. GF14 genes in rice response to biotic and abiotic stress has also been well addressed. The role of GF14e in rice defense has been well studied. However, whether Hd3a and OsFD1 play roles in defense is unclear. In present study, we identified that Hd3a and OsFD1 expression is repressed by Xoo and JA, and validated that Hd3a and OsFD1 negatively regulate resistance to Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc). hd3a and osfd1 mutants increase resis...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Ke Y, Wu M, Zhang Q, Li X, Xiao J, Wang S Tags: Biochem Biophys Res Commun Source Type: research

CACNB2 is associated with aberrant RAS-MAPK signaling in hypertensive Dahl Salt-Sensitive rats.
Abstract Several independent genome-wide association studies (GWAS) have indicated that calcium (Ca2+) voltage-gated channel auxiliary subunit beta 2 (CACNB2) an L-type Ca2+ channel (LTCC) associated protein has strong association with hypertension. However, the molecular mechanism of CACNB2 and its role in the pathophysiology of hypertension is not clear. To address this knowledge gap, we utilized in vitro and in vivo approaches using HEK293 cells and genetically hypertensive, Dahl Salt-Sensitive (SS) rats. We demonstrated that CACNB2 over-expression in HEK293 cells triggers cell proliferation via a...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Durairaj Pandian V, Giovannucci DR, Vazquez G, Kumarasamy S Tags: Biochem Biophys Res Commun Source Type: research

Modulation of the cell cycle regulating transcription factor E2F1 pathway by the proteasome following amino acid starvation.
Abstract The proteasome is one of the main catalytic machineries of eukaryotic cells responsible for protein degradation, and is known to be regulated during several cellular stress conditions. Recent studies suggest that the activity of the proteasome is modulated following mTOR inhibition. However, it is not clear how this process affects the proteome. In the present study, we investigated the role of the proteasome in the modulation of the proteome of HeLa cells following amino acid starvation, a stress known to inhibit mTOR activity. We used label-free quantitative proteomics to identify proteins regulated by ...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Fabre B, Livneh I, Ziv T, Ciechanover A Tags: Biochem Biophys Res Commun Source Type: research

Distinct roles of Deinococcus radiodurans RecFOR and RecA in DNA transformation.
Abstract RecFOR and RecA are key recombination factors in Deinococcus radiodurans, a bacterium that possesses robust DNA repair capability and is also naturally transformable. While RecFOR functioning as a RecA loader during DNA repair has been established, their relative roles in transformation need further exploration. Here, we constructed recFOR and recA deletion mutants of D. radiodurans, and investigated the effect of these mutations on DNA transformation. recA deletion causes defects in both plasmid and chromosomal transformation. However, it was found that recFOR is not involved in chromosomal transfor...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Wang Y, Xu G, Wang L, Hua Y Tags: Biochem Biophys Res Commun Source Type: research

Intrauterine low-protein diet disturbs metanephric gene expression and induces urinary tract developmental abnormalities in mice.
In this study, we reported that intrauterine low-protein diet can cause congenital anomalies of the kidney and urinary tract (CAKUT) phenotypes, which was dominated by the duplicated collecting system phenotype. At the same time, ectopic ureteric buds were increased under intrauterine low-protein diet and the number of UB branches was reduced in the serum-free culture. Intrauterine low-protein diet can change metanephric gene expression. Slit2/Robo2 and Spry1 expression levels were decreased, Ret expression was increased, and downstream p-Akt activity enhanced with apoptosis abnormal in ureteric bud tissue, which may be th...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Yu M, Tan L, Chen J, Zhai Y, Wu X, Xu H, Shen Q Tags: Biochem Biophys Res Commun Source Type: research

Protein interactome of the deamidase phosphoribosylformylglycinamidine synthetase (PFAS) by LC-MS/MS.
This study is first to report the interaction of PFAS with several proteins which play physiological roles in tumor development including CAD, CCT2, PRDX1, and PHGDH. Furthermore, we show here that PFAS is able to deamidate PHGDH, and induce other posttranslational modification into CAD, CCT2 and PRDX1. The present data provide insight on the biological function of PFAS. Further study to explore the role of these protein interactions in tumorigenesis and other diseases is recommended. PMID: 30987822 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Lu A, Disoma C, Zhou Y, Chen Z, Zhang L, Shen Y, Zhou M, Du A, Zheng R, Li S, Alsaadawe M, Li S, Li J, Wang W, Jiang T, Peng J, Xia Z Tags: Biochem Biophys Res Commun Source Type: research

Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae).
Abstract The eukaryotic ribosomal DNA (rDNA) cluster consists of multiple copies of three genes (18S, 5.8S, and 28S rDNA) and two internal transcribed spacers (ITS1 and ITS2). Typically, rDNA is thought to be evolved in a strict concerted evolution pattern, in which show little or even no variations within species, while sequence diversity between species or higher taxa. However, in the present study, we reported a high level of intra-individual polymorphism of ITS1-5.8S-ITS2 rDNA in the genome of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae), suggesting it conformed a non-concerted evolutionary pattern...
Source: Biochemical and Biophysical Research communications - April 13, 2019 Category: Biochemistry Authors: Gong L, Luo H, Shi W, Yang M Tags: Biochem Biophys Res Commun Source Type: research

In  vitro differentiation of mouse T cell-derived hybrid cells obtained through cell fusion with embryonic stem cells.
In vitro differentiation of mouse T cell-derived hybrid cells obtained through cell fusion with embryonic stem cells. Biochem Biophys Res Commun. 2019 Apr 12;: Authors: Nakajima M, Suzuki T, Hara T, Kitajima K Abstract Nuclear reprogramming is an innovative advance in cell biology. An important research initiative in this field is cell fusion-mediated nuclear reprogramming, wherein the nuclei of somatic cells, such as thymocytes, are initialized through cell fusion with embryonic stem cells (ESCs). However, hybrid cells obtained through cell fusion between ESCs and thymocytes failed to contribute...
Source: Biochemical and Biophysical Research communications - April 12, 2019 Category: Biochemistry Authors: Nakajima M, Suzuki T, Hara T, Kitajima K Tags: Biochem Biophys Res Commun Source Type: research

Construction of small molecular CTLA4 analogs with CD80-binding affinity.
Abstract A variety of CTLA4-Fc fusion proteins and anti-CTLA4 monoclonal antibody have been approved. Given the shortcomings of macromolecular antibodies, recombinant proteins derived from the tenth unit of human type III fibronectin (FN3) termed monobody were studied as CTLA4 analogs in this study. A peptide EL161 derived from CD80-binding domain (MYPPPY motifs) in the complementarity determining region (CDR) 3 of CTLA4 was found to inhibit the interaction of CTLA4 with CD80 significantly. Afterward, the peptide EL16 as well as the CDR1 of CTLA4 which is also critical for its binding to CD80 were grafted onto FN3...
Source: Biochemical and Biophysical Research communications - April 12, 2019 Category: Biochemistry Authors: Liu F, Su L, Chen Z, Feng D, Wei J, Sun J Tags: Biochem Biophys Res Commun Source Type: research

Identification of a potent inhibitor of type II secretion system from Pseudomonas aeruginosa.
Abstract Pseudomonas aeruginosa is an opportunistic pathogen infecting human population. The pathogen is becoming a serious health problem due to its ability to evade normal immune response of the host and multiple drug resistance to many antibiotics. The pathogen has 2 major virulence systems of which the type III secretion system (T3SS) is of major concern to humans. A third system, type 2 secretion system (T2SS), is common to bacteria and used to secrete exotoxin A (ExoA) responsible for human cell destruction. To help bypass the drug resistance, a strategy to block the T2SS based on a low similarity between hu...
Source: Biochemical and Biophysical Research communications - April 12, 2019 Category: Biochemistry Authors: Swietnicki W, Czarny A, Antkowiak L, Zaczynska E, Kolodziejczak M, Sycz J, Stachowicz L, Alicka M, Marycz K Tags: Biochem Biophys Res Commun Source Type: research

Pellino1 specifically binds to phospho-Thr18 of p53 and is recruited to sites of DNA damage.
This study provides evidence that Pellino1 translocates to damaged DNA in the nucleus and has a functional role in p53 signaling and the DNA damage response. PMID: 30987826 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 12, 2019 Category: Biochemistry Authors: Dai L, Lin J, Said AB, Yau YH, Shochat SG, Ruiz-Carrillo D, Sun K, Chandrasekaran R, Sze SK, Lescar J, Cheung PC Tags: Biochem Biophys Res Commun Source Type: research

Mitofusin 2 is involved in chemotaxis of neutrophil-like differentiated HL-60  cells.
Mitofusin 2 is involved in chemotaxis of neutrophil-like differentiated HL-60 cells. Biochem Biophys Res Commun. 2019 Apr 12;: Authors: Mazaki Y, Takada S, Nio-Kobayashi J, Maekawa S, Higashi T, Onodera Y, Sabe H Abstract Neutrophils rapidly migrate to infection sites after the recognition of invaders. During chemotaxis, neutrophils require energy supplied by mitochondria oxidative phosphorylation (OXPHOS), whereas neutrophils rely heavily on glycolysis under normal conditions. Mitochondrial OXPHOS correlates with mitochondrial morphology. Here, we examined the mitochondrial morphology of neutrophil...
Source: Biochemical and Biophysical Research communications - April 12, 2019 Category: Biochemistry Authors: Mazaki Y, Takada S, Nio-Kobayashi J, Maekawa S, Higashi T, Onodera Y, Sabe H Tags: Biochem Biophys Res Commun Source Type: research

Fucoxanthin exerts anti-fibrogenic effects in hepatic stellate cells.
The objective of this study was to evaluate whether fucoxanthin (FCX) have anti-fibrogenic properties in hepatic stellate cells (HSCs). FCX significantly decreased basal and transforming growth factor β1 (TGFβ1)-induced mRNA levels of fibrogenic genes with concomitant decreases in their protein levels in LX-2 cells. The phosphorylation of SMA- and MAD-related protein (SMAD3) was increased by TGFβ1, which was attenuated by FCX. Importantly, when LX-2 cells were treated with FCX and SIS3, a SMAD3 inhibitor, there was synergistic repression of fibrogenic gene expression. The anti-fibrogenic effect of FCX wa...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Kim MB, Bae M, Hu S, Kang H, Park YK, Lee JY Tags: Biochem Biophys Res Commun Source Type: research

Ad5-EMC6 mediates antitumor activity in gastric cancer cells through the mitochondrial apoptosis pathway.
In this study, we report that adenovirus-mediated ectopic overexpression of EMC6 (Ad5-EMC6) in BGC823 and SGC7901 gastric cancer cells decreases the activity of ERK1/2, down-regulates the levels of BCL-2 protein and phosphorylated BCL-2, increases the expression of tBID and BAX, and decreases mitochondrial membrane potential and subsequently leading to cell apoptosis. In a xenograft tumor model, we found that Ad5-EMC6 impairs the tumorigenesis of SGC7901 gastric cancer cells in nude mice. Additionally, Ad5-EMC6 enhances the sensitivity of gastric cancer cells to the chemotherapeutic drug etoposide. Collectively, these resu...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Li R, Wang X, Zhang X, Yu J, Feng J, Lv P, Lou Y, Chen Y Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA MNX1-AS1 promotes hepatocellular carcinoma proliferation and invasion through targeting miR-218-5p/COMMD8 axis.
In this study, we found that MNX1-AS1 was remarkably upregulated in HCC tissues and cell lines. Furthermore, MNX1-AS1 overexpression was related to advanced stage and metastasis, and predicted poor prognosis. Loss-of-function assays showed that MNX1-AS1 knockdown suppressed the proliferation, migration and invasion of HCC cells in vitro. Further investigation indicated that MNX1-AS1 silencing delayed HCC growth in vivo. Mechanistically, we identified that MNX1-AS1 was a competing endogenous RNA (ceRNA) for miR-218-5p. We demonstrated that MNX1-AS1 promoted COMMD8 expression through sponging miR-218-5p, and then c...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Ji D, Wang Y, Sun B, Yang J, Luo X Tags: Biochem Biophys Res Commun Source Type: research

Biodelivery of nerve growth factor and gold nanoparticles encapsulated in chitosan nanoparticles for schwann-like cells differentiation of human adipose-derived stem cells.
Abstract The constant release of neurotrophic factors through a nanomaterial-based delivery system can be an important strategy in medical and pharmaceutical fields for nerve tissue engineering. The present study was aimed at encapsulating NGF and AuNPs in chitosan nanoparticles (NGF-CNPs and AuNPs-CSNPs) and its evaluation on the differentiation potential of human adipose-derived stem cells (h-ADSCs) to Schwann-like cells. The NGF-CNPs were prepared by ionotropic gelation method with tripolyphosphate (TPP) as a crosslinker. After synthesis and characterization of nanoparticles, NGF encapsulation efficiency and re...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Razavi S, Seyedebrahimi R, Jahromi M Tags: Biochem Biophys Res Commun Source Type: research

Disruption of SpoIIID decreases sporulation, increases extracellular proteolytic activity and virulence in Bacillus anthracis.
Abstract Endospores are important for maintenance of the B. anthracis lifecycle and necessary for its effective spread between hosts. Our experiments with B. anthracis showed that disruption of SpoIIID results in a spore formation defect, as determined by heat resistance assays and microscopic assessment. We further found complete engulfment by the ΔspoIIID mutant strain by membrane morphology staining but no synthesis of the clarity coat and exosporium by transmission electron microscopy. Reduced transcription and expression of small acid-soluble spore protein(sasP-2) and the spore development ass...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Lyu Y, Gu M, Chen M, Feng E, Zhu L, Pan C, Wang D, Liu X, Wang H Tags: Biochem Biophys Res Commun Source Type: research

NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1 β processing during Streptococcus pneumoniae infection.
In this study, mouse peritoneal neutrophils from C57BL/6 WT and inflammasome components knockout mice were infected by S. pneumoniae in vitro. The results showed that NLRP3 inflammasome is critically involved in neutrophil IL-1β secretion, while the AIM2 and NLRC4 inflammasomes were dispensable. Moreover, the upstream kinase, JNK, modulates ASC oligomerization and consequent caspase-1 activation and IL-1β secretion. Additionally, neutrophil serine proteases also participate in IL-1β secretion by mediating ASC oligomerization and caspase-1 activation. Taken together, these findings indicated that bo...
Source: Biochemical and Biophysical Research communications - April 11, 2019 Category: Biochemistry Authors: Zhang T, Du H, Feng S, Wu R, Chen T, Jiang J, Peng Y, Ye C, Fang R Tags: Biochem Biophys Res Commun Source Type: research

Gonadotropin regulates NUCB2/nesfatin-1 expression in the mouse ovary and uterus.
Abstract NUCB2/nesfatin-1 is expressed in the hypothalamus and regulates food intake and energy metabolism. Recent studies showed that NUCB2/nesfatin-1 also plays a role in other organs. However, its expression pattern and function in female reproductive organs are unclear. Therefore, we investigated NUCB2/nesfatin-1 expression in the ovary and uterus of mice and determined whether it is regulated by gonadotropins and sex steroid hormones. NUCB2 mRNA and nesfatin-1 protein were detected in the ovary and uterus of mice. NUCB2/nesfatin-1 expression in both organs was highest in the estrus period of the estrus cycle....
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Kim J, Sun S, Lee D, Youk H, Yang H Tags: Biochem Biophys Res Commun Source Type: research

Oleic acid-induced defective autolysosome shows impaired lipid degradation.
In this study, we evaluated the effect of oleic acid (OA) on lipophagy in cells. We found that OA treatment results in accumulation of p62 and LC3-II proteins and reduces red fluorescence in cells stably expressing mCherry-GFP-LC3. In addition, OA inhibits the co-localization of LC3 with LAMP1 under serum-deprived condition, suggesting that OA blocks autophagosome-lysosome fusion. In the cells with ATG5 or ULK1 gene deletion, LDs did not increase upon OA treatment more than in wild type cells. However, cell starvation following OA removal resulted in reduced lipid accumulation by lipophagy and recovery of autophagy flux, s...
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Lee DH, Ahn J, Jang YJ, Ha TY, Jung CH Tags: Biochem Biophys Res Commun Source Type: research

Nr4a1 as a myogenic factor is upregulated in satellite cells/myoblast under proliferation and differentiation state.
Abstract Myogenic differentiation is precisely regulated with a cascade of genes and pathways. The previous study has demonstrated the muscle-specific deletion of Nr4a1 impairs muscle growth. However, it is still unclear whether muscular Nr4a1 deletion may directly impact myoblast physiology. Here, the present study delves into the molecular mechanism of Nr4a1 in C2C12. Through the analysis of RNAseq and microarray data, Nr4a1 was identified to highly correlate with the expression of myogenic factors. In C2C12, except confirming the induction of Nr4a1 mRNA and protein levels upon the initiation of differentiation,...
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Pan X, Liu B, Chen S, Ding H, Yao X, Cheng Y, Xu D, Yin Y, Dai X, Sun J, Xu G, Pan J, Xiao L, Xie L Tags: Biochem Biophys Res Commun Source Type: research

PFKFB3/HIF-1 α feedback loop modulates sorafenib resistance in hepatocellular carcinoma cells.
PFKFB3/HIF-1α feedback loop modulates sorafenib resistance in hepatocellular carcinoma cells. Biochem Biophys Res Commun. 2019 Apr 10;: Authors: Long Q, Zou X, Song Y, Duan Z, Liu L Abstract Hepatocellular carcinoma (HCC) is a prevalent malignancy with increasing incidence and extremely poor prognosis worldwide. The multi-kinase inhibitor sorafenib is widely used as a first-line systematic treatment agent of advanced hepatocellular carcinoma. However, the benefit of sorafenib in clinical treatment is often impeded by drug resistance. Therefore, it is of critical importance to investigate the mol...
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Long Q, Zou X, Song Y, Duan Z, Liu L Tags: Biochem Biophys Res Commun Source Type: research

Lipopolysaccharide protects against acetaminophen-induced hepatotoxicity by reducing oxidative stress via the TNF- α/TNFR1 pathway.
Lipopolysaccharide protects against acetaminophen-induced hepatotoxicity by reducing oxidative stress via the TNF-α/TNFR1 pathway. Biochem Biophys Res Commun. 2019 Apr 10;: Authors: Zhao S, Sheng D, Shi R, Jing Y, Jiang J, Meng Y, Fu Z, Hou X, Liu W, Yang X, Li R, Han Z, Wei L Abstract Robust evidence suggested that gut-derived lipopolysaccharide (LPS) plays a significant role in various liver injury diseases; however, the role of gut-derived LPS in acetaminophen (APAP) overdose-induced acute liver injury remains unclear. The present study aimed to investigate the effect of gut-derived LPS on AP...
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Zhao S, Sheng D, Shi R, Jing Y, Jiang J, Meng Y, Fu Z, Hou X, Liu W, Yang X, Li R, Han Z, Wei L Tags: Biochem Biophys Res Commun Source Type: research

Sirtuin 2 expression suppresses oxidative stress and senescence of nucleus pulposus cells through inhibition of the p53/p21 pathway.
Abstract Intervertebral disc degeneration (IDD) is a kind of disease associated with nucleus pulposus (NP) cell senescence. Previous studies have shown that the sirtuin family plays an extremely important role in the progress of cell aging. However, whether sirtuin2 (Sirt2) protects against IDD remains unknown. The aim of this study was to determine whether Sirt2 protected NP from degradation in IDD. The expression of Sirt2 in different degree of degenerate disc tissues was determined by reverse transcription-polymerase chain reaction. Interleukin 1 beta (IL-1β) was used to stimulate the degeneration of NP ce...
Source: Biochemical and Biophysical Research communications - April 10, 2019 Category: Biochemistry Authors: Yang M, Peng Y, Liu W, Zhou M, Meng Q, Yuan C Tags: Biochem Biophys Res Commun Source Type: research