Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2.
Abstract Sulindac sulfone is a metabolite of sulindac, a non-steroidal anti-inflammatory drug (NSAID), without anti-inflammatory ability. However, sulindac sulfone has been reported to significantly reduce polyps in patients with colorectal adenomatous polyposis in clinical trials. Thus, sulindac sulfone is expected to be useful for the chemoprevention of neoplasia with few side effects related to anti-inflammatory ability. To date, the molecular targets of sulindac sulfone have not yet fully investigated. Therefore, in order to newly identify sulindac sulfone-binding proteins, we generated sulindac sulfone-fixed ...
Source: Biochemical and Biophysical Research communications - October 13, 2018 Category: Biochemistry Authors: Aono Y, Horinaka M, Iizumi Y, Watanabe M, Taniguchi T, Yasuda S, Sakai T Tags: Biochem Biophys Res Commun Source Type: research

Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice.
Abstract Mammalian target of rapamycin complex 1 (mTORC1) is a major regulator of cell growth and proliferation through fuel sensing. Systemic inhibition of mTOR as well as manipulation of its downstream products prevent diet-induced obesity. The purpose of this study was to determine the consequences of intestine-targeted mTORC1 inhibition. To attenuate intestinal mTORC1 activity, Villin-CreER mice were crossed with Raptorflox/flox mice, creating an intestinal-specific Raptor null line (i-Raptor -/-). Mice were fed a high fat diet (HFD) and compositional changes as well as food intake levels were assessed. O...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Onufer EJ, Tay S, Barron LK, Courtney CM, Warner BW, Guo J Tags: Biochem Biophys Res Commun Source Type: research

Targeting ninjurin 2 by miR-764 regulates hydrogen peroxide (H2O2)-induced neuronal cell death.
Abstract Ninjurin 2 (NINJ2) is a novel adhesion molecule expressed in neurons and glial cells. The current study determined if specific microRNA (miRNA) can regulate NINJ2 expression in human neuronal cells. Sequence analysis of NINJ2 mRNA 3'-untranslated region (3'-UTR) revealed that microRNA-764 (miR-764) putatively targets NINJ2. In SH-SY5Y/SK-N-BE neuronal cells and primary human neurons, lentivirus-mediated overexpression of miR-764 conferred significant repression on NINJ2 3'-UTR luciferase activity and downregulation of NINJ2 mRNA/protein. Conversely, transfection of the miR-764 inhibitor increased NINJ2 mR...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Jing D, Yinzhu L, Jinjing P, Lishuang L, Guozhuan Z Tags: Biochem Biophys Res Commun Source Type: research

Astrocytes in the mouse visual cortex reliably respond to visual stimulation.
In conclusion, our research demonstrated that astrocytes in the primary visual cortex of awake mice responded to visual stimuli with a delay of several seconds relative to the surrounding neurons, which may indicate the existence of a common mechanism of neuron-astrocyte communication across species. PMID: 30322614 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Sonoda K, Matsui T, Bito H, Ohki K Tags: Biochem Biophys Res Commun Source Type: research

MLN64 deletion suppresses RANKL-induced osteoclastic differentiation and attenuates diabetic osteoporosis in streptozotocin (STZ)-induced mice.
In conclusion, the results in our study suggested that MLN64 played a critical role in the meditation of osteoclastic differentiation, and its suppression alleviated diabetic osteoporosis in STZ-induced mice. Thus, MLN64 could be served as an essential target for developing effective therapeutic strategy to prevent diabetic osteoporosis. PMID: 30322615 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Lei C, Xueming H, Ruihang D Tags: Biochem Biophys Res Commun Source Type: research

miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.
Abstract microRNAs (miRNAs) control several processes known to be involved in progression of aneurysm. Here, intracranial aneurysms (IAs) were surgically induced in Sprague-Dawley rats, and we found that miR-448-3p was downregulated and KLF5 was upregulated in IA rats. We identified Klf5 as a direct target of miR-448-3p in smooth muscle cells (SMCs). In addition, aneurysms size and the lumen area of the aneurysms were smaller 4 weeks after IA induction in the miR-448-3p-treated group. miR-448-3p treatment protected the wall thickness ratio and suppressed macrophage infiltration after IA induction. IAs caused a sig...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Zhang JZ, Chen D, Lv LQ, Xu Z, Li YM, Wang JY, Han KW, Yu MK, Huang CG, Hou LJ Tags: Biochem Biophys Res Commun Source Type: research

DDX21 promotes gastric cancer proliferation by regulating cell cycle.
In this study, we observed that DDX21 was significantly up-regulated in gastric cancer tissues compared to paired adjacent normal tissues. The expression of DDX21 was closely related to the pathological stage of gastric cancer. In vitro and in vivo studies had shown that knockdown of DDX21 inhibited gastric cancer cell proliferation, colony formation, G1/S cell cycle transition and xenograft growth, while ectopic expression of DDX21 promoted these cell functions. Mechanically, DDX21 induced gastric cancer cell growth by up-regulating levels of Cyclin D1 and CDK2. Taken together, these results revealed a novel rol...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Cao J, Wu N, Han Y, Hou Q, Zhao Y, Pan Y, Xie X, Chen F Tags: Biochem Biophys Res Commun Source Type: research

The collagen-derived compound collagen tripeptide induces collagen expression and extends lifespan via a conserved p38 mitogen-activated protein kinase cascade.
In this study, we examined the effects of collagen tripeptide (CTP), a collagen-derived compound, on lifespan and aging. Our results showed that CTP upregulated collagen genes via the p38 mitogen-activated protein kinase (MAPK)/SKN-1 pathway. Moreover, CTP extended lifespan and delayed aging through p38 MAPK/SKN-1 pathway. In addition, CTP also induced collagen expression via the p38 MAPK pathway in mammals. Our findings supported that external stimuli such as CTP could promote ECM youthfulness using a conserved signaling pathway, thereby contributing to suppression of aging. PMID: 30322618 [PubMed - as supplied by pu...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Morikiri Y, Matsuta E, Inoue H Tags: Biochem Biophys Res Commun Source Type: research

Structural basis for multiple gene regulation by human DUX4.
Abstract DUX4 plays critical role in the molecular pathogenesis of the neuromuscular disorder facioscapulohumeral muscular dystrophy and acute lymphoblastic leukemia in humans. As a master transcription regulator, DUX4 can also bind the promoters and activate the transcription of hundreds ZGA-associated genes. Here we report on the structural and biochemical studies of DUX4 double homeodomains (DUX4-DH), representing the only structures contain both homeodomain 1 (HD1) and homeodomain 2 (HD2). HD1 and HD2 adopt classical homeobox fold; via the helix inserted into the major groove and the N-terminal extended loop i...
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Li Y, Wu B, Liu H, Gao Y, Yang C, Chen X, Zhang J, Chen Y, Gu Y, Li J, Ma J, Gan J Tags: Biochem Biophys Res Commun Source Type: research

SAPAP4 Deletion Causes Synaptic Dysfunction in the nucleus accumbens.
Abstract SAP90/PSD95-associated proteins (SAPAPs) are one type of scaffold protein in the postsynaptic density (PSD). Scaffold proteins play an important role in synaptic function. Recently, many studies have shown that mutations associated with scaffold proteins cause dysfunction in neuronal circuitry and in behavior. SAPAP4, as a protein in the SAPAP family, may have an impact on synaptic functions and on behaviors. To test this hypothesis, mice with a genetic deletion of SAPAP4 were used in our study. SAPAP4-/- mice displayed decreased cocaine sensitivity behavior after an acute injection of 20 mg/kg cocaine....
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Wu B, Li C, Lei H Tags: Biochem Biophys Res Commun Source Type: research

Zoledronate and high glucose levels influence osteoclast differentiation and bone absorption via the AMPK pathway.
In conclusion, the results suggest AMPK has potential as a new target for the treatment of osteoporosis, the high glucose environment promoted RAW264.7 cell migration, but suppressed osteoclast differentiation and bone absorption, and ZOL suppressed osteoclast migration, differentiation, and bone absorption via the AMPK pathway. PMID: 30322621 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 12, 2018 Category: Biochemistry Authors: Dong W, Qi M, Wang Y, Feng X, Liu H Tags: Biochem Biophys Res Commun Source Type: research

"Biochem Biophys Res Commun"; +237 new citations
237 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Biochem Biophys Res Commun" These pubmed results were generated on 2018/10/11PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 11, 2018 Category: Biochemistry Tags: Report Source Type: research

Editorial for BBRC lipidomics special issue.
PMID: 30293583 [PubMed - in process] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 7, 2018 Category: Biochemistry Authors: Spiegel S, Ntambi J Tags: Biochem Biophys Res Commun Source Type: research

Co3O4 nanoparticles at sublethal concentrations inhibit cell growth by impairing mitochondrial function.
This study uncovers a mitochondrial respiratory chain-related and ROS-independent toxicity mechanism of Co3O4 NPs in eukaryotic cells. PMID: 30293680 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 5, 2018 Category: Biochemistry Authors: Wang H, Ren T, Zhu N, Yu Q, Li M Tags: Biochem Biophys Res Commun Source Type: research

Three-dimensional model of intracellular and intercellular Ca2+ waves propagation in endothelial cells.
In this study, we developed a three-dimensional (3D) simulation model for intercellular and intracellular Ca2+ waves in endothelial cells (ECs). 3D model of 2 cells was reconstructed from confocal microscopic images and was connected via gap junctions. Cells have membrane and cytoplasm domains, and metabolic reactions were divided into each domain. Finally, the intracellular and intercellular Ca2+ wave propagations were induced using microscopic stimulation and were compared between numerical simulations and experiments. The experiments showed that initial sharp increases in intracellular Ca2+ occurred approximately 0.3...
Source: Biochemical and Biophysical Research communications - October 5, 2018 Category: Biochemistry Authors: Sera T, Komine S, Arai M, Sunaga Y, Yokota H, Kudo S Tags: Biochem Biophys Res Commun Source Type: research

Crystal structure of an ENT domain from Trypanosoma brucei.
Abstract Trypanosoma brucei (T. brucei) is a parasitic protozoan causing human sleeping sickness and related animal diseases. ENT (EMSY N-terminal) domain was first found in EMSY protein which has been proved to be involved in multiple biological processes such as DNA repair, tumorigenesis, and transcriptional regulation. So far, little is known about the function and structure of ENT domains from protozoan. Q385P5 from T. brucei, containing an ENT domain at its N-terminus, is a conserved protein in related kinetoplastid parasites. In this work, the crystal structure of ENT domain of Q385P5 (TbENT) was s...
Source: Biochemical and Biophysical Research communications - October 4, 2018 Category: Biochemistry Authors: Mi J, Yang X, Zhang J, Zhang X, Xu C, Liao S, Tu X Tags: Biochem Biophys Res Commun Source Type: research

Corrigendum to "A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1" [Biochem. Biophys. Res. Commun. 504 (1) (2018 Sep 26) 328-333].
Corrigendum to "A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1" [Biochem. Biophys. Res. Commun. 504 (1) (2018 Sep 26) 328-333]. Biochem Biophys Res Commun. 2018 Oct 04;: Authors: Mussakhmetov A, Shumilin IA, Nugmanova R, Shabalin IG, Baizhumanov T, Toibazar D, Khassenov B, Minor W, Utepbergenov D PMID: 30293683 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 4, 2018 Category: Biochemistry Authors: Mussakhmetov A, Shumilin IA, Nugmanova R, Shabalin IG, Baizhumanov T, Toibazar D, Khassenov B, Minor W, Utepbergenov D Tags: Biochem Biophys Res Commun Source Type: research

NFAT5 promotes in  vivo development of murine melanoma metastasis.
In this study, we knocked down NFAT5 to investigate its role in melanoma cancer. shRNA-mediated knockdown of NFAT5 led to a significant decrease in cell proliferation in vitro. Additionally, depletion of NFAT5 inhibited the cell migratory ability of B16BL6 melanoma cells and led to more accumulation at the G2/M phase of the cell cycle. Furthermore, NFAT5 was essential for the development of melanoma cancer pathophysiology in an in vivo mouse model. NFAT5 knockdown-induced tumor growth was slow and tumor volume was significantly reduced compared to mock controls. Moreover, NFAT5 knockdown was associated with a low...
Source: Biochemical and Biophysical Research communications - October 4, 2018 Category: Biochemistry Authors: Kim DH, Kim KS, Ramakrishna S Tags: Biochem Biophys Res Commun Source Type: research

Oral administration of indole substituted dipyrido[2,3-d]pyrimidine derivative exhibits anti-tumor activity via inhibiting AKT and ERK1/2 on hepatocellular carcinoma.
In this study, we synthesized a series of novel indole substituted dihydropyrido[2,3-d]pyrimidines through the multicomponent reactions to connect pyrido[2,3-d]pyrimidine and indole moities via an one-pot three-component reaction of 3-cyanoacetyl indoles 1, various aromatic aldehyde 2, and 2,6-diaminopyrimidin-4(3H)-one 3. Subsequently, we screened their cytotoxicity via CCK-8 assay in HepG2 cells, a human hepatoma cell line and chose compound 4p that showed the lowest dosage of IC50 to study the antitumor activities to HCC. Interestingly, 4p significantly induced the cell cycle arrest and apoptosis of HepG2 via targeting ...
Source: Biochemical and Biophysical Research communications - October 4, 2018 Category: Biochemistry Authors: Gao X, Cen L, Li F, Wen R, Yan H, Yao H, Zhu S Tags: Biochem Biophys Res Commun Source Type: research

Novel effect of sildenafil on hair growth.
CONCLUSION: Our study demonstrates for the first time, the significant therapeutic potential of sildenafil on hair growth and its potential use in treatment of alopecia. PMID: 30292404 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Choi HI, Kang BM, Jang J, Hwang ST, Kwon O Tags: Biochem Biophys Res Commun Source Type: research

MFSD2A expression predicts better prognosis in gastric cancer.
Abstract Major facilitator superfamily domain containing-2A (MFSD2A) is reported to correlated with some tumors, but its clinical significance in gastric cancer (GC) is still unknown. The expression of MFSD2A and CD34 were examined on tissue microarrays of 170 set of GC and adjacent normal tissue (ANT) by immunohistochemistry. The relationship of MFSD2A with microvessel density (MVD) and clinicopathological characteristics was also investigated. MFSD2A expression is lower in GC tissue (35.3%) than in ANT (78.2%, P 
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Shi X, Huang Y, Wang H, Zheng W, Chen S Tags: Biochem Biophys Res Commun Source Type: research

Sterol regulatory element binding protein-1: Molecular cloning, tissue distribution and gene expression level in response to nutritional regulation in mud crab, Scylla paramamosain.
Abstract In the present study, SREBP-1 cDNA was cloned from the hepatopancreas of mud crab (Scylla paramamosain) and characterized by performing rapid-amplification of cDNA ends. The 3361bp long full-length cDNA encodes a polypeptide with 1039 amino acids. Tissue distribution analysis revealed that SREBP-1 transcripts were widely distributed in various organs, with higher mRNA levels in the eyestalk and cranial ganglia. Further, expression level of SREBP-1 mRNA were up-regulated in proportion to the replacement of dietary fish oil (FO) with soybean oil (SO). These results may contribute to better understanding of ...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Hao M, Lin Z, Rong H, Zhu D, Wen X Tags: Biochem Biophys Res Commun Source Type: research

MicroRNA-182-5p attenuates cerebral ischemia-reperfusion injury by targeting Toll-like receptor 4.
Abstract Cerebral ischemia-reperfusion-induced microglial activation causes neuronal death through the release of inflammatory cytokines. Increasing evidence suggests that microRNAs (miRNAs) exert a neuroprotective effect by modulating the inflammatory process in cerebral ischemia-reperfusion injury. Furthermore, Toll-like receptor 4 (TLR4) is increasingly being considered to have a significant role in the regulation of inflammation. However, whether miRNAs mediate their neuroprotective effects by regulating TLR4-mediated inflammatory responses remains unknown. To explore this gap in the literature, we conducted b...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Wang J, Xu Z, Chen X, Li Y, Chen C, Wang C, Zhu J, Wang Z, Chen W, Xiao Z, Xu R Tags: Biochem Biophys Res Commun Source Type: research

Kin1 kinase localizes at the hyphal septum and is dephosphorylated by calcineurin but is dispensable for septation and virulence in the human pathogen Aspergillus fumigatus.
Abstract Studies in yeasts have implicated the importance of Kin1 protein kinase, a member of the eukaryotic PAR1/MARK/MELK family, in polarized growth, cell division and septation through coordinated activity with the phosphatase, calcineurin. Kin1 is also required for virulence of the fungal pathogens Cryptococcus neoformans and Fusarium graminearum. Here we show that kin1 deletion in the human fungal pathogen Aspergillus fumigatus does not affect hyphal growth and septation but results in differential susceptibility to antifungals targeting the cell wall and cell membrane. The Δkin1 strain remained virule...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Juvvadi PR, Cole DC, Falloon K, Waitt G, Soderblom EJ, Moseley MA, Steinbach WJ Tags: Biochem Biophys Res Commun Source Type: research

Development of trophoblast cystic structures from human induced pluripotent stem cells in limited-area cell culture.
Abstract We developed a novel engineering technique to induce differentiation of human induced pluripotent stem cells (hiPSCs) into organoids mimicking the trophectoderm (TE). Here, hiPSCs were cultured on a limited area of 2-4 mm in diameter. After 15-20 days, spherical cysts appeared on the surface of the limited area. Secretion of human chorionic gonadotrophin (hCG) began to increase after ∼ 20 days and remained dramatically elevated over the next 20 days. Limited-area-cultured cysts exhibited expression of hCG, which was a result of epithelial differentiation. Low expression levels of pluripotent gen...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Li Z, Kurosawa O, Iwata H Tags: Biochem Biophys Res Commun Source Type: research

Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in  vitro and in vivo.
Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo. Biochem Biophys Res Commun. 2018 Oct 03;: Authors: Jia C, Kong D, Guo Y, Li L, Quan L Abstract Bortezomib (BTZ) is one of the most frequently used drugs in treatment of multiple myeloma (MM), but drug-resistance often occurs and limits its clinical efficacy. Annexin A1 (ANXA1) is upregulated in MM, and its knockdown enhances chemosensitivity in MM. However, whether ANXA1 inhibition can increase antitumor activity of BTZ in MM cells remains unknown. In the presen...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Jia C, Kong D, Guo Y, Li L, Quan L Tags: Biochem Biophys Res Commun Source Type: research

A combination of irinotecan/cisplatinum and irinotecan/temozolomide or tumor-targeting Salmonella typhimurium A1-R arrest doxorubicin- and temozolomide-resistant myxofibrosarcoma in a PDOX mouse model.
ngh SR, Zhao M, Hoffman RM Abstract Myxofibrosarcoma (MFS) is the most common sarcomas in elderly patients and is either chemo-resistant or recurs with metastasis after chemotherapy. This recalcitrant cancer in need of improved treatment. We have established a patient-derived orthotopic xenograft (PDOX) of MFS. The MFS PDOX model was established in the biceps femoris of nude mice and randomized into 7 groups of 7 mice each: control; doxorubicin (DOX); pazopanib (PAZ); temozolomide (TEM); Irinotecan (IRN); IRN combined with TEM; IRN combined with cisplatinum (CDDP) and Salmonella typhimurium A1-R (S. typhimuri...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Kiyuna T, Tome Y, Murakami T, Miyake K, Igarashi K, Kawaguchi K, Oshiro H, Higuchi T, Miyake M, Sugisawa N, Zhang Z, Razmjooei S, Wangsiricharoen S, Chmielowski B, Nelson SD, Russell TA, Dry SM, Li Y, Eckardt MA, Singh AS, Chawla S, Kanaya F, Eilber FC, S Tags: Biochem Biophys Res Commun Source Type: research

A role of GUNs-Involved retrograde signaling in regulating Acetyl-CoA carboxylase 2 in Arabidopsis.
In conclusion, our results suggested that the GUN-involved plastid-to-nucleus retrograde communication played a role in regulating ACC2 in Arabidopsis. PMID: 30292412 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Wang J, Xia H, Zhao SZ, Hou L, Zhao CZ, Ma CL, Wang XJ, Li PC Tags: Biochem Biophys Res Commun Source Type: research

Inflammatory production of reactive oxygen species by Drosophila hemocytes activates cellular immune defenses.
Abstract The production of reactive oxygen species (ROS) is a prominent response to infection among innate immune cells such as macrophages and neutrophils. To better understand the relationship between antimicrobial and regulatory functions of blood cell ROS, we have characterized the ROS response to infection in Drosophila hemocytes. Using fluorescent probes, we find a biphasic hemocyte ROS response to bacterial infection. In the first hour, virtually all hemocytes generate a transient ROS signal, with nonphagocytic cells including prohemocytes and crystal cells displaying exceptionally strong responses. A disti...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Myers AL, Harris CM, Choe KM, Brennan CA Tags: Biochem Biophys Res Commun Source Type: research

LncRNA-CIR promotes articular cartilage degeneration in osteoarthritis by regulating autophagy.
This study aims to analyze the roles of long noncoding RNA (lncRNA)CIR and autophagy in cartilages and determine their overall contribution to the degradation of extracellular matrix. Patients with OA possessed high levels of lncRNA-CIR and MMP3 and low level of COL2A1. The levels of autophagy-related proteins, including LC3BI/II and beclin-1, increased from 12 h to 48 h. The use of si-lncRNA-CIR reversed the trend compared with that in the OA group. The negative effect of lncRNA-CIR was assessed in vivo by establishing a model of surgically induced OA. Moreover, si-lncRNA-CIR-treated joints exhibited fewer OA cha...
Source: Biochemical and Biophysical Research communications - October 3, 2018 Category: Biochemistry Authors: Wang CL, Peng JP, Chen XD Tags: Biochem Biophys Res Commun Source Type: research

Adherens junction-associated pores mediate the intercellular transport of endosomes and cytoplasmic proteins.
Abstract Intercellular endosomes (IEs) are endocytosed vesicles shuttled through the adherens junctions (AJs) between two neighboring epidermal cells during Drosophila dorsal closure. The cell-to-cell transport of IEs requires DE-cadherin (DE-cad), microtubules (MTs) and kinesin. However, the mechanisms by which IEs can be transported through the AJs are unknown. Here, we demonstrate the presence of AJ-associated pores with MTs traversing through the pores. Live imaging allows direct visualization of IEs being transported through the AJ-associated pores. By using an optogenetic dimerization system, we observe that...
Source: Biochemical and Biophysical Research communications - October 2, 2018 Category: Biochemistry Authors: Yang WT, Li YC, Lin CM, Tang WC, Chen BC, Suzuki E, Hsu JC Tags: Biochem Biophys Res Commun Source Type: research

Aspergillus fumigatus phospholipase D may enhance reactive oxygen species production by accumulation of histone deacetylase 6.
Abstract Aspergillus fumigatus, an airborne pathogen, causes many diseases, including aspergilloma, invasive aspergillosis, and allergic bronchopulmonary aspergillosis. Phospholipase D (PLD) is an important virulence factor for A. fumigatus infection, but the manner by which PLD contributes to the virulence of this pathogen is not clear. Our results show that expression of A. fumigatus PLD in human cells was able to increase the production of reactive oxygen species (ROS), which play an important role in several signaling pathways as well as in lung infection. Meanwhile, A. fumigatus PLD was found t...
Source: Biochemical and Biophysical Research communications - October 1, 2018 Category: Biochemistry Authors: Yu S, Huo K Tags: Biochem Biophys Res Commun Source Type: research

The role of UNC5b in ox-LDL inhibiting migration of RAW264.7 macrophages and the involvement of CCR7.
Abstract The formation of macrophage foam cells by ingesting ox-LDL and focal retention in the subendothelial space are the hallmarks of the early atherosclerotic lesion. The C-C chemokine receptor type 7 (CCR7) is positively correlated with the macrophage migration. But the mechanism of CCR7 regulation is not fully clearness. In the present study, we demonstrates that expression in UNC5b and netrin-1 was enhanced in respond to ox-LDL in Raw264.7 macrophage and associated with decreasing cell migration. Interestingly, it was found that ox-LDL significantly downregulate CCR7 gene expression. The expression of CCR7 ...
Source: Biochemical and Biophysical Research communications - October 1, 2018 Category: Biochemistry Authors: Yang X, Zhang J, Chen L, Yuan Z, Qin X, Wu Q, Shen D, He H, Yu C Tags: Biochem Biophys Res Commun Source Type: research

Serum thyroid hormones levels are significantly decreased in pregnant rats with acute pancreatitis.
In this study, maternal thyroid injury as well as pancreas injury occurred in a time-dependent manner. The activities of serum amylase, lipase and levels of MDA, TNF-α and IL-1β were markedly increased in acute pancreatitis rats, the levels of serum FT3 and FT4 were obviously decreased in APIP groups, and the expressions of MIF, ICAM-1 and CD68 were significantly increased in the thyroid of the APIP group. Ultrastructural thyroid injuries were observed in the APIP group. Our research suggests that thyroid injury is involved in the rat experimental model of APIP. The degree of thyroid dysfunction is associated wi...
Source: Biochemical and Biophysical Research communications - October 1, 2018 Category: Biochemistry Authors: Wang CY, Zhao L, Hong YP, Mei FC, Zhou Y, Shi Q, Guo WY, Deng WH, Chen C, Yu J, Wang WX Tags: Biochem Biophys Res Commun Source Type: research

Intrathecal circHIPK3 shRNA alleviates neuropathic pain in diabetic rats.
We present the first evidence that intrathecal circHIPK3 shRNA treatment can be used to treat neuropathic pain of diabetic rats. PMID: 30286957 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - October 1, 2018 Category: Biochemistry Authors: Wang L, Luo T, Bao Z, Li Y, Bu W Tags: Biochem Biophys Res Commun Source Type: research

β1,6 GlcNAc branches-modified protein tyrosine phosphatase Mu attenuates its tyrosine phosphatase activity and promotes glioma cell migration through PLCγ-PKC pathways.
In this study, We found that GnT-V expression was negatively correlated with receptor protein tyrosine phosphatase type μ(RPTPμ) in human glioma tissues. To study whether RPTPμ is a novel substance of GnT-V which further affect RPTPμ's downstream dephosphorylation function, we preform lentiviral infection with GnT-V gene to construct stably transfected GnT-V glial cell lines. We found RPTPμ undergone severer cleavage in GnT-V transfected glioma cells compare to Mock cells. RPTPμ intracellular domain fragments increased while β1,6-GlcNAc-branched N-glycans increased, in consistent with the decrease of...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Gao Y, Yang F, Su Z, He Z, Xiao J, Xu Y, Zha X, Xu F, Wang L Tags: Biochem Biophys Res Commun Source Type: research

DNA polymerase β deficiency in the p53 null cerebellum leads to medulloblastoma formation.
DNA polymerase β deficiency in the p53 null cerebellum leads to medulloblastoma formation. Biochem Biophys Res Commun. 2018 Sep 29;: Authors: Kim J, Kim J, Lee Y Abstract Defects in DNA damage response or repair mechanisms during neurogenesis result in genomic instability, which is causative for several neural defects. These include brain tumors, particularly medulloblastoma, which occurs in the cerebellum with a high incidence in children. We generated an animal model with defective base excision repair during brain development through selective inactivation of DNA polymerase β (Polb) in ne...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Kim J, Kim J, Lee Y Tags: Biochem Biophys Res Commun Source Type: research

Long noncoding RNA LOXL1-AS1 regulates prostate cancer cell proliferation and cell cycle progression through miR-541-3p and CCND1.
Abstract Prostate cancer is one of the most frequent malignancies affecting men. Long non-coding RNAs (lncRNAs) are involved in the pathogenesis of prostate cancer. LncRNA LOXL1-AS1 participates in the pathogenesis of the exfoliation syndrome. However, the role of LOXL1-AS1 in cancer remains largely unknown. Here, we found that LOXL1-AS1 down-regulation inhibited prostate cancer cell proliferation and cell cycle progression. RNA sequencing analysis revealed that it regulates the expression of cell cycle-related genes. LOXL1-AS1 is predominantly distributed in the cytoplasm, where it interacts with miR-541-3p. In a...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Long B, Li N, Xu XX, Li XX, Xu XJ, Liu JY, Wu ZH Tags: Biochem Biophys Res Commun Source Type: research

BAF53A regulates androgen receptor-mediated gene expression and proliferation in LNCaP cells.
Abstract The actin-like protein of the SWI/SNF complex, BAF53A, regulates gene expression by the gene-specific chromatin remodeling of target genes. However, the function of BAF53A in the androgen receptor pathway in prostate cancer cells remains unclear. Here, we demonstrated that BAF53A positively regulates the expression of endogenous AR target genes (e.g. PSA, TMPRSS2, FKBP5, and KLK2) in LNCaP cells. It functions as a coactivator in AR-mediated transcription by interacting with other nuclear receptor coactivators, such as p300 and FLII, and is associated with AR in the presence of dihydrotestosterone (DHT). T...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Jin ML, Kim YW, Jeong KW Tags: Biochem Biophys Res Commun Source Type: research

Selective toxicity of caffeic acid in hepatocellular carcinoma cells.
In this study we found caffeic acid (CA) but not ferulic, sinapic or cinnamic acids inhibited proliferation of hepatocellular carcinoma cells (HCC) and reduced cell numbers by inducing apoptosis. Only transient exposure to CA was required for these lethal effects that are associated with disruption of mitochondrial membrane potential and induction of reactive oxygen species. By comparison, primary hepatocytes resisted CA toxicity for nearly 48 h, consistent with selective sensitivity of HCC to CA. These results support use of CA as an anti-tumor agent to inhibit HCC, especially if delivered by locoregional catheterizatio...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Brautigan DL, Gielata M, Heo J, Kubicka E, Wilkins LR Tags: Biochem Biophys Res Commun Source Type: research

Ferricrocin, the intracellular siderophore of Trichoderma virens, is involved in growth, conidiation, gliotoxin biosynthesis and induction of systemic resistance in maize.
Abstract Fungal siderophores are known to be involved in iron acquisition and storage, as well as pathogenicity of mammals and plants. As avirulent plant symbionts, Trichoderma spp. colonize roots and induce resistance responses both locally and systemically. To study the role of intracellular siderophore(s) in Trichoderma-plant interactions, we have obtained mutants in a non-ribosomal peptide synthetase, TvTex10, that was predicted to be involved in intracellular siderophore(s) biosynthesis. This gene has a detectable basal level of expression and is also upregulated under iron-deplete conditions. This is unlike ...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Mukherjee PK, Hurley JF, Taylor JT, Puckhaber L, Lehner S, Druzhinina I, Schumacher R, Kenerley CM Tags: Biochem Biophys Res Commun Source Type: research

Maternal supplementation with calcium varying with feeding time daily during late pregnancy affects lipid metabolism and transport of placenta in pigs.
Abstract To investigate effects of Ca level varying with feeding time daily in sows during late pregnancy on placental lipid metabolism and transport in pigs, sixty pregnant sows were assigned to 3 groups: the CON group was fed low-Ca diet with 11.25 g CaCO3 at 0600 h and 1500 h, H-L group was fed low-Ca diet with 22.5 g CaCO3 at 0600 h and low-Ca diet at 1500 h, and L-H group was fed low-Ca diet at 0600 h and low-Ca diet with 22.5 g CaCO3 at 1500 h, respectively. Serum from sows and umbilical cord and placenta were collected during delivery. Results showed that, compared with the CON group, H-L ...
Source: Biochemical and Biophysical Research communications - September 29, 2018 Category: Biochemistry Authors: Gao LM, Xie CY, Zhang TY, Wu X, Yin YL Tags: Biochem Biophys Res Commun Source Type: research

The galectin LEC-5 is a novel binding partner for RAB-11.
Abstract RAB-11/Rab11 is an endosomal GTPase with conserved roles in directional trafficking and apical domain formation in polarized epithelial cells. From a yeast two-hybrid screen using full-length C. elegans RAB-11 as bait, we identified LEC-5 as a novel binding protein for RAB-11. LEC-5 is an ortholog of mammalian Galectin-9 which associates with glycosphingolipids and is implicated in apical cargo sorting. We further confirmed the interaction between RAB-11 and LEC-5 via GST-pull down, co-immunoprecipitation and bimolecular fluorescence complementation. In addition, we showed that LEC-5 binds to RAB-11 ...
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Zhang N, Wang X, Gobel V, Zhang X Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA OGFRP1 regulates LYPD3 expression by sponging miR-124-3p and promotes non-small cell lung cancer progression.
Abstract Long noncoding RNA (OGFRP1) has been reported to be involved in the progression of non-small cell lung cancer (NSCLC). However, the expression pattern, functions and molecular mechanisms of OGFRP1 in NSCLC remains unclear. In the present study, we found that OGFRP1 expression was significantly up-regulated in both NSCLC tissues and cell lines, and the upregulation of OGFRP1 expression is a powerful predictor of advanced clinical stage, lymph nodes metastasis and poor prognosis for NSCLC patients. Loss-of-function assay indicated that knockdown of OGFRP1 inhibited proliferation, migration and invasion, and...
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Tang LX, Chen GH, Li H, He P, Zhang Y, Xu XW Tags: Biochem Biophys Res Commun Source Type: research

Protective effect of electro-acupuncture at maternal different points on perinatal nicotine exposure-induced pulmonary dysplasia in offspring based on HPA axis and signal transduction pathway.
In this study, we compared the different effect of ST 36 and "Fenglong" (ST 40), which belong to the same meridian, in terms of lung pulmonary function and morphology, PPARγ, β-catenin, GR levels in the lung tissues and CORT in the serum of perinatal nicotine exposure offspring, and explored the mechanism of acupuncture based on the maternal hypothalamus-pituitary-adrenal (HPA) axis. It is shown that EA applied to ST 36 could restore the normal function of maternal HPA axis and alleviate maternal glucocorticoid overexposure in offspring, thereby it can up-regulate the PTHrP/PPARγ and down-regulat...
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Liu Y, Ji B, Zhao G, Su H, Ge Y, Dai J, Lu Y, Sakurai R, Rehan VK Tags: Biochem Biophys Res Commun Source Type: research

MiR-374b-5p-FOXP1 feedback loop regulates cell migration, epithelial-mesenchymal transition and chemosensitivity in ovarian cancer.
In conclusion, miR-374b-5p-FOXP1 feedback loop regulates tumor progression and chemosensitivity in ovarian cancer. PMID: 30274777 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Li H, Liang J, Qin F, Zhai Y Tags: Biochem Biophys Res Commun Source Type: research

Low dose Emodin induces tumor senescence for boosting breast cancer chemotherapy via silencing NRARP.
CONCLUSION: These findings provide preclinical evidence for repurposing use of Emodin in combination with chemotherapeutic agents to treat breast cancer as an alternative salvage regimen. PMID: 30274778 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Zu C, Qin G, Yang C, Liu N, He A, Zhang M, Zheng X Tags: Biochem Biophys Res Commun Source Type: research

Induced protein degradation of anaplastic lymphoma kinase (ALK) by proteolysis targeting chimera (PROTAC).
Abstract Recently, proteolysis targeting chimera (PROTAC) technology is highlighted in drug discovery area as a new therapeutic approach. PROTAC as a heterobifunctional molecule is comprised of two ligands, which recruit target protein and E3 ligase, respectively. To degrade the anaplastic lymphoma kinase (ALK) fusion protein, such as NPM-ALK or EML4-ALK, we generated several ALK-PROTAC molecules consisted of ceritinib, one of the ALK inhibitors, and ligand of von Hippel-Lindau (VHL) E3 ligase. Among these molecules, TD-004 effectively induced ALK degradation and inhibited the growth of ALK fusion positive cell li...
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Kang CH, Lee DH, Lee CO, Du Ha J, Park CH, Hwang JY Tags: Biochem Biophys Res Commun Source Type: research

SDF-1/CXCR4 axis promotes the growth and sphere formation of hypoxic breast cancer SP cells by c-Jun/ABCG2 pathway.
In conclusion, CXCR4 increases the growth and SFE of breast cancer SP cells under hypoxia through c-Jun-mediated transcriptional activation of ABCG2. PMID: 30274780 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: He C, Zhang H, Wang B, He J, Ge G Tags: Biochem Biophys Res Commun Source Type: research

Evaluation of collagen mixture on promoting skin wound healing in zebrafish caused by acetic acid administration.
Abstract The aim of this study is to use zebrafish embryos as a quick platform for wound healing studies. At beginning, we optimized a protocol to induce skin lesion by acetic acid injection. The acetic acid injection induced regional inflammation wound hyperpigmentation by recruiting pigment cells to the wound area. Later, we applied established platform to evaluate the effect of tilapia's collagen peptide mixtures, including demonstration on promoting skin wound healing and eliminating inflammatory response. Results showed that after treating TY001, one of the above fish collagen peptide mixtures, not only repai...
Source: Biochemical and Biophysical Research communications - September 28, 2018 Category: Biochemistry Authors: Xiong XY, Liu Y, Shan LT, Xu YQ, Liang J, Lai YH, Hsiao CD Tags: Biochem Biophys Res Commun Source Type: research