Genetic deletion of miR-204 improves glycemic control despite obesity in db/db mice.
In conclusion, the absence of miR-204 improves β-cell proliferation, decreases islet ER stress, and improves glycemic control with limited change in body weight in obese mice. PMID: 32950230 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 16, 2020 Category: Biochemistry Authors: Gaddam RR, Kim YR, Li Q, Jacobs JS, Gabani M, Mishra A, Promes JA, Imai Y, Irani K, Vikram A Tags: Biochem Biophys Res Commun Source Type: research

Cognition in some surprising places.
;ka F Abstract The most widely accepted view in the biopsychological sciences is that the cognitive functions that are diagnostic of mental operations, sentience or, more commonly, consciousness emerged fairly late in evolution, most likely in the Cambrian period. Our position dovetails with James's below - subjectivity, feeling, consciousness has a much longer evolutionary history, one that goes back to the first appearance of life. The Cellular Basis of Consciousness (CBC) model is founded on the presumption that sentience and life are coterminous; that all organisms, based on inherent cellular activit...
Source: Biochemical and Biophysical Research communications - September 16, 2020 Category: Biochemistry Authors: Reber AS, Baluška F Tags: Biochem Biophys Res Commun Source Type: research

Pathogenic mutations perturb calmodulin regulation of Nav1.8 channel.
Abstract The voltage-gated sodium channels play a key role in the generation and propagation of the cardiac action potential. Emerging data indicate that the Nav1.8 channel, encoded by the SCN10A gene, is a modulator of cardiac conduction and variation in the gene has been associated with arrhythmias such as atrial fibrillation (AF) and Brugada syndrome (BrS). The voltage gated sodium channels contain a calmodulin (CaM)-binding IQ domain involved in channel slow inactivation, we here investigated the role of CaM regulation of Nav1.8 channel function, and showed that CaM enhanced slow inactivation of the Nav1.8 cha...
Source: Biochemical and Biophysical Research communications - September 15, 2020 Category: Biochemistry Authors: Hong L, Zhang M, Sridhar A, Darbar D Tags: Biochem Biophys Res Commun Source Type: research

Mitochondria transfer from tumor-activated stromal cells (TASC) to primary Glioblastoma cells.
M, Oliver L Abstract The tumor microenvironment (TME) controls many aspects of cancer development but little is known about its effect in Glioblastoma (GBM), the main brain tumor in adults. Tumor-activated stromal cell (TASC) population, a component of TME in GBM, was induced in vitro by incubation of MSCs with culture media conditioned by primary cultures of GBM under 3D/organoid conditions. We observed mitochondrial transfer by Tunneling Nanotubes (TNT), extracellular vesicles (EV) and cannibalism from the TASC to GBM and analyzed its effect on both proliferation and survival. We created primary cultures of...
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Salaud C, Alvarez-Arenas A, Geraldo F, Belmonte-Beitia J, Calvo GF, Gratas C, Pecqueur C, Garnier D, Pérez-Garcià V, Vallette FM, Oliver L Tags: Biochem Biophys Res Commun Source Type: research

Implication of Staphylococcus aureus MsrB dimerization upon oxidation.
This study elucidated the structure of MsrB from Staphylococcus aureus Mu50 and its changes upon oxidation. The active site shows two reduced cysteines in a close contact, implying disulfide bond would form without major structural rearrangement. When the protein is exposed to an oxidative condition, a dimeric state is observed. The dimerization of SAMsrB creates a valley structure for accepting peptidyl substrates. To the best of our knowledge, oxidation induced dimerization of SAMsrB would help to understand mechanism behind redox control that has not been well characterized. PMID: 32943184 [PubMed - as supplied by ...
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Kim HJ Tags: Biochem Biophys Res Commun Source Type: research

Structural analysis of the sensor domain of the β-lactam antibiotic receptor VbrK from Vibrio parahaemolyticus.
Structural analysis of the sensor domain of the β-lactam antibiotic receptor VbrK from Vibrio parahaemolyticus. Biochem Biophys Res Commun. 2020 Sep 14;: Authors: Cho SY, Yoon SI Abstract Bacteria express β-lactamase to counteract the bactericidal effects of β-lactam antibiotics, which are the most widely employed antibacterial drugs. In gram-negative bacteria, the expression of β-lactamase is generally regulated in response to the muropeptide that is generated from the peptidoglycan of the cell wall during β-lactam antibiotic challenge. The direct regulation of β-lactama...
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Cho SY, Yoon SI Tags: Biochem Biophys Res Commun Source Type: research

Chronic high fat diet impairs glucagon like peptide-1 sensitivity in vagal afferents.
This study aimed to examine the effect of chronic high fat diet on GLP-1 sensitivity in vagal afferents. C57/BL6 mice were fed either a high-fat or low-fat diet for 6-8 weeks. To evaluate gastrointestinal afferent sensitivity and nodose neurons' response to GLP-1, extracellular afferent recordings and patch clamp were performed, respectively. Exendin-4 (Ex-4) was used as an agonist of the GLP-1 receptor. C-Fos Expression was examined as an indication of afferent input to the nucleus tractus solitarius (NTS). Food intake was monitored in real-time before and after Ex-4 treatment to monitor the consequence of the high fat di...
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Al Helaili A, Park SJ, Beyak MJ Tags: Biochem Biophys Res Commun Source Type: research

1,2-Dilinoleoyl-sn-glycero-3-phosphocholine increases insulin sensitivity in palmitate-treated myotubes and induces lipolysis in adipocytes.
In conclusion, DLPC enhanced lipolysis and apoptosis via a TNFα-dependent pathway in adipocytes and attenuated palmitate-induced insulin resistance through PPARα-mediated suppression of inflammation in myocytes. PMID: 32943187 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Park J, Jung TW, Chung YH, Park ES, Jeong JH Tags: Biochem Biophys Res Commun Source Type: research

Structural characterization and cryo-electron tomography analysis of human islet amyloid polypeptide suggest a synchronous process of the hIAPP1-37 amyloid fibrillation.
Abstract Revealing the aggregation and fibrillation process of variant amyloid proteins is critical for understanding the molecular mechanism of related amyloidosis diseases. Here we characterized the fibrillation morphology and kinetics of type 2 diabetes (T2D) related human islet amyloid polypeptide (hIAPP1-37) fibril formation process using negative staining transmission electron microscopy (NS-TEM), cryo-electron microscopy (cryo-EM) analysis, and 3D cryo-electron tomography (cryo-ET) reconstruction, together with circular dichroism (CD) and Thioflavin-T (ThT) assays. Our results showed that various amyloid fi...
Source: Biochemical and Biophysical Research communications - September 14, 2020 Category: Biochemistry Authors: Zhang X, Li D, Zhu X, Wang Y, Zhu P Tags: Biochem Biophys Res Commun Source Type: research

The p105 NF- ĸB precursor is a pseudo substrate of the ubiquitin ligase FBXO7, and its binding to the ligase stabilizes it and results in stimulated cell proliferation.
The p105 NF-ĸB precursor is a pseudo substrate of the ubiquitin ligase FBXO7, and its binding to the ligase stabilizes it and results in stimulated cell proliferation. Biochem Biophys Res Commun. 2020 Sep 12;: Authors: Udasin RG, Gottfried Y, Fabre B, Bercovich B, Ziv T, Ciechanover A Abstract The NF-κB transcription factor is involved in inflammation and cell proliferation, survival, and transformation. It is a heterodimer made of p50 or p52 and a member of the Rel family of proteins. p50 and p52 are derived from limited ubiquitin- and proteasome-mediated proteolytic processing of the larger p...
Source: Biochemical and Biophysical Research communications - September 12, 2020 Category: Biochemistry Authors: Udasin RG, Gottfried Y, Fabre B, Bercovich B, Ziv T, Ciechanover A Tags: Biochem Biophys Res Commun Source Type: research

Arabidopsis NRT1.2 interacts with the PHOSPHOLIPASE D α1 (PLDα1) to positively regulate seed germination and seedling development in response to ABA treatment.
In this study, we demonstrate that NRT1.2 positively regulated the ABA response during germination and seedling development. The transgenic Arabidopsis NRT1.2-over-expressionors showed increased sensitivity to ABA during these processes. qRT-PCR assays indicated that NRT1.2 over-production in 7-days-old seedlings up-regulated the expression of ABA-responsive genes: ABI1, ABI2, ABI3, ABI4, ABI5, RAB18, RD29A, and RD29B and PHOSPHOLIPASE Dα1 (PLDα1). The expression of these genes was suppressed in the nrt1.2 mutant in comparison with the wild type following ABA treatment. Importantly, bimolecular fluorescence com...
Source: Biochemical and Biophysical Research communications - September 12, 2020 Category: Biochemistry Authors: Li J, Zhao C, Hu S, Song X, Lv M, Yao D, Song Q, Zuo K Tags: Biochem Biophys Res Commun Source Type: research

Identification of ribavirin-responsive cis-elements for GPAM suppression in the GPAM genome.
Abstract Glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) is a rate-limiting enzyme catalyzing triglyceride synthesis. Recently, we demonstrated that the anti-viral drug ribavirin (RBV) reduces GPAM expression by downregulating CCAAT/enhancer-binding protein α (C/EBPα). However, the precise mechanisms of GPAM suppression have remained unclear. Here, we found that RBV suppressed GPAM expression by downregulating not only C/EBPα, but also sterol regulatory element-binding protein-1c (SREBP-1c). We also found that cis-elements regulated by C/EBPα and SREBP-1c functioned as distal and...
Source: Biochemical and Biophysical Research communications - September 12, 2020 Category: Biochemistry Authors: Onomura D, Satoh S, Ueda Y, Dansako H, Kato N Tags: Biochem Biophys Res Commun Source Type: research

Improved differentiation of human adipose stem cells to insulin-producing β-like cells using PDFGR kinase inhibitor Tyrphostin9.
In this study, we developed a modified conversion protocol with improved yield and functionality, which is achieved by changing the culture method and addition of Tyrphostin9, a platelet-derived growth factor receptor (PDGFR) kinase inhibitor. Tyrphostin9 was identified from a cell-based chemical screening using the mCherry reporter under the control of the Pdx1 promoter. The β-like cells differentiated under the new protocol showed a 3.6-fold increase in the expression of Pdx1, a marker for pancreatic differentiation, as compared to the previous protocol. We propose that Tyrphostin9 contributes to the β-like cel...
Source: Biochemical and Biophysical Research communications - September 12, 2020 Category: Biochemistry Authors: Mandal P, De D, Yun K, Kim KK Tags: Biochem Biophys Res Commun Source Type: research

Inchworm stepping of Myc-Max heterodimer protein diffusion along DNA.
Abstract Oncogenic protein Myc serves as a transcription factor to control cell metabolisms. Myc dimerizes via leucine zipper with its associated partner protein Max to form a heterodimer structure, which then binds target DNA sequences to regulate gene transcription. The regulation depends on Myc-Max binding to DNA and searching for target sequences via diffusional motions along DNA. Here, we conduct structure-based molecular dynamics (MD) simulations to investigate the diffusion dynamics of the Myc-Max heterodimer along DNA. We found that the heterodimer protein slides on the DNA in a rotation-uncoupled manner i...
Source: Biochemical and Biophysical Research communications - September 12, 2020 Category: Biochemistry Authors: Dai L, Yu J Tags: Biochem Biophys Res Commun Source Type: research

G protein subunit β1 is an important mediator of the late stage of endochondral ossification.
This study was designed to investigate the role of GNB1 in skeletal development using Gnb1-KO mice. Gnb1-KO mice showed dwarfism, shortening of limbs, and a decreased ossifying zone of long bones. In situ hybridization and RT-qPCR analyses revealed that Col10a1 and Mmp13 expression was reduced in long bones of Gnb1-KO mice, while Runx2, Osterix, Ihh, and Ppr expression levels were similar to those in wild-type littermates. Gnb1-KO-derived osteoblasts maintained calcification abilities and the expression levels of osteoblast marker genes were unaltered, indicating that osteoblast differentiation and function were not affect...
Source: Biochemical and Biophysical Research communications - September 11, 2020 Category: Biochemistry Authors: Ruengsinpinya L, Murakami T, Nakamura E, Takahata Y, Hata K, Nakaminami Y, Okae H, Nishimura R Tags: Biochem Biophys Res Commun Source Type: research

Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature.
Abstract Temperature governs states and dynamics of all biological molecules, and several cellular processes are often heat sources and/or sinks. Technical achievement of intracellular thermometry enables us to measure intracellular temperature, and it can offer novel perspectives in biology and medicine. However, little is known that changes of intracellular temperature throughout the cell-cycle and the manner of which cells regulates their thermogenesis in response to fluctuation of the environmental temperature. Here, cell-cycle-dependent changes of intracellular temperature were reconstructed from the snapshot...
Source: Biochemical and Biophysical Research communications - September 11, 2020 Category: Biochemistry Authors: Yamanaka R, Shindo Y, Hotta K, Hiroi N, Oka K Tags: Biochem Biophys Res Commun Source Type: research

Novel Sca-1+ macrophages modulate the pathogenic progress of endotoxemia.
Abstract Macrophages are important innate immune cells that play crucial roles in inflammatory responses. Accumulating evidence has demonstrated macrophage heterogeneity based on biomarkers, functions, and localization. Here, we report a novel stem cell antigen-1 (Sca-1)-positive macrophage population induced in the pathological conditions caused by lipopolysaccharide (LPS). Sca-1 is only upregulated in macrophages but not in monocytes and neutrophils upon LPS injection. Sca-1+ macrophages develop from resident peritoneal macrophages. LPS-induced Sca-1+ macrophage generation was partly blocked by anti-IFN-γ ...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Park MY, Kim HS, Jeong YS, Kim HY, Bae YS Tags: Biochem Biophys Res Commun Source Type: research

Corrigendum to "25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p ˮ.
Corrigendum to "25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5pˮ. Biochem Biophys Res Commun. 2020 Sep 10;: Authors: Zhang L, Lv Y, Xian G, Lin Y PMID: 32921410 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Zhang L, Lv Y, Xian G, Lin Y Tags: Biochem Biophys Res Commun Source Type: research

N-glycosylation of Siglec-15 decreases its lysosome-dependent degradation and promotes its transportation to the cell membrane.
Abstract Siglec-15 was recently reported to be an immunosuppressive molecule that is expressed by tumor-associated macrophages and upregulated in some solid tumors. Targeting Siglec-15 is a potential strategy for normalization cancer immunotherapy. Here, we identified the important post-translational modification, N-glycosylation of Siglec-15, which is regulated by glucose uptake. Using a series of glycosidase and glycosylation inhibitors, we demonstrated that Siglec-15 was completely N-glycosylated in vitro and in vivo. The precise glycosylation site was determined. N-glycosylation stabilized Siglec-15 ...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Chen X, Dang X, Song J, Wang G, Liu C, Cui L, Huang Z Tags: Biochem Biophys Res Commun Source Type: research

Brave new world revisited: Focus on nanomedicine.
Abstract Nanomedicine is at a crossroads: with relatively few success stories in terms of clinical translation despite more and more research on increasingly sophisticated nanomaterials, it is important to consider whether we are on the right track. Indeed, it is crucial that we address the fact that while considerable efforts are being made to overcome barriers to translation from the bench to the clinic, scientists are still struggling to decipher fundamental aspects of nanomaterial interactions with biological systems. We believe that a key to the successful adoption of nanomedicines in oncology and beyond lies...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Fadeel B, Alexiou C Tags: Biochem Biophys Res Commun Source Type: research

A hypothesis for insulin resistance in primary human adipocytes involving MRTF-A and suppression of PPAR γ.
A hypothesis for insulin resistance in primary human adipocytes involving MRTF-A and suppression of PPARγ. Biochem Biophys Res Commun. 2020 Sep 10;: Authors: Hansson B, Schumacher S, Fryklund C, Morén B, Björkqvist M, Swärd K, Stenkula KG Abstract Obesity is the main risk factor behind insulin resistance and type 2 diabetes. Still, the mechanism behind adipocyte dysfunction is not yet resolved. Recently, we reported that rapid actin remodeling correlates with adipose cell size changes after short-term overfeeding. Therefore, we hypothesized that the actin-driven myocardin-relate...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Hansson B, Schumacher S, Fryklund C, Morén B, Björkqvist M, Swärd K, Stenkula KG Tags: Biochem Biophys Res Commun Source Type: research

Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution. The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively. These results suggest that IgG is advantageous for generating binders against spacious epitopes such as gl...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Ogasawara S, Suzuki K, Naruchi K, Nakamura S, Shimabukuro J, Tsukahara N, Kaneko MK, Kato Y, Murata T Tags: Biochem Biophys Res Commun Source Type: research

Pseudomonas donghuensis HYS 7-hydroxytropolone contributes to pathogenicity toward Caenorhabditis elegans and is influenced by pantothenic acid.
Abstract Pseudomonas donghuensis HYS, a bacterial strain identified from Donghu Lake, has tremendous toxicity toward Caenorhabditis elegans and is characterized by high 7-hydroxytropolone siderophore production. Here, the relationship between pathogenic siderophore production and pantothenic acid was evaluated. The pathogenicity of P. donghuensis HYS was illustrated using C. elegans as a host. Based on slow-killing assay findings, a 7-hydroxytropolone deficiency-causing mutation attenuated P. donghuensis HYS pathogenicity, which was restored by the addition of extracted 7-hydroxytropolone. Moreover, data...
Source: Biochemical and Biophysical Research communications - September 10, 2020 Category: Biochemistry Authors: Gui Z, You J, Xie G, Qin Y, Wu T, Xie Z Tags: Biochem Biophys Res Commun Source Type: research

Acidic electrolyzed water potently inactivates SARS-CoV-2 depending on the amount of free available chlorine contacting with the virus.
Abstract Alcohol-based disinfectant shortage is a serious concern in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Acidic electrolyzed water (EW) with a high concentration of free available chlorine (FAC) shows strong antimicrobial activity against bacteria, fungi, and viruses. Here, we assessed the SARS-CoV-2-inactivating efficacy of acidic EW for use as an alternative disinfectant. The quick virucidal effect of acidic EW depended on the concentrations of contained-FAC. The effect completely disappeared in acidic EW in which FAC was lost owing to long-time storage after generation. In...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Takeda Y, Uchiumi H, Matsuda S, Ogawa H Tags: Biochem Biophys Res Commun Source Type: research

SARS-CoV-2 E protein is a potential ion channel that can be inhibited by Gliclazide and Memantine.
In conclusion, as a route to curb viral virulence and abate COVID-19, we point to the E protein of SARS-CoV-2 as an attractive drug target and identify off-label compounds that inhibit it. PMID: 32828269 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Singh Tomar PP, Arkin IT Tags: Biochem Biophys Res Commun Source Type: research

Enhanced cellular uptake of CpG DNA by α-helical antimicrobial peptide Kn2-7: Effects on macrophage responsiveness to CpG DNA.
This study examined the effects of four α-helical antimicrobial peptides on the immune responses induced by CpG DNA. The antimicrobial peptide FIKRIARLLRKIF, known as Kn2-7, increased the CpG DNA-dependent secretion of interleukin-10 (IL-10) and tumor necrosis factor-α from mouse macrophage-like RAW264.7 cells. Kn2-7 enhanced the cellular uptake of CpG DNA; this effect was decreased by the substitution of arginine residues with alanine residues, and increased by the substitution of lysine residues with arginine residues. The degree to which these peptides enhanced the cellular uptake of CpG DNA correlated ...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Nishihara S, Kawasaki K Tags: Biochem Biophys Res Commun Source Type: research

AAV9-DOK7 gene therapy reduces disease severity in Smn2B/- SMA model mice.
on CL Abstract Spinal Muscular Atrophy (SMA) is an autosomal recessive neuromuscular disease caused by deletions or mutations in the survival motor neuron (SMN1) gene. An important hallmark of disease progression is the pathology of neuromuscular junctions (NMJs). Affected NMJs in the SMA context exhibit delayed maturation, impaired synaptic transmission, and loss of contact between motor neurons and skeletal muscle. Protection and maintenance of NMJs remains a focal point of therapeutic strategies to treat SMA, and the recent implication of the NMJ-organizer Agrin in SMA pathology suggests additional NMJ organizi...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Kaifer KA, Villalón E, Smith CE, Simon ME, Marquez J, Hopkins AE, Morcos TI, Lorson CL Tags: Biochem Biophys Res Commun Source Type: research

Hippo pathway cooperates with ChREBP to regulate hepatic glucose utilization.
Abstract Hippo pathway plays a crucial role as a regulator of organ size and tumorigenesis that negatively regulates cell growth and survival. Recently lots of evidences show that Hippo pathway plays a crucial role in glucose metabolic metabolism to regulate energy status with cell growth. However, the detailed mechanism is still unclear. Here we report that Yes-associated protein (YAP), the terminal effector of Hippo pathway, interacts with carbohydrate response element binding protein (ChREBP) in the nucleus of the hepatocytes thereby promoting glycolysis and lipogenesis. A high carbohydrate (HCHO) diet could in...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Shu ZP, Yi GW, Deng S, Huang K, Wang Y Tags: Biochem Biophys Res Commun Source Type: research

Activation of NF- κB by TOPK upregulates Snail/Slug expression in TGF-β1 signaling to induce epithelial-mesenchymal transition and invasion of breast cancer cells.
Activation of NF-κB by TOPK upregulates Snail/Slug expression in TGF-β1 signaling to induce epithelial-mesenchymal transition and invasion of breast cancer cells. Biochem Biophys Res Commun. 2020 Sep 10;530(1):122-129 Authors: Lee YJ, Park JH, Oh SM Abstract TGF-β1 is known to induce epithelial-mesenchymal transition (EMT), which is a prerequisite for cancer cell invasion. Here we reveal that TOPK upregulates EMT and invasion of human breast cancer MDA-MB-231 or Hs578T cells via NF-κB-dependent Snail/Slug in TGF-β1 signaling. Endogenous TOPK expression was significantly incr...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Lee YJ, Park JH, Oh SM Tags: Biochem Biophys Res Commun Source Type: research

Unique synaptic topography of crest-type synapses in the interpeduncular nucleus.
In conclusion, our data reveals a hitherto unknown unique topographical feature of crest synapses in the IPN. PMID: 32828274 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Parajuli LK, Wako K, Maruo S, Kakuta S, Koike M Tags: Biochem Biophys Res Commun Source Type: research

CoAsy knockdown in TNBC cell lines resulted in no overt effect on cell proliferation in  vitro.
CoAsy knockdown in TNBC cell lines resulted in no overt effect on cell proliferation in vitro. Biochem Biophys Res Commun. 2020 Sep 10;530(1):136-141 Authors: Kharabsheh HA, Scott JE Abstract Triple-negative breast cancer (TNBC) remains the most challenging breast cancer subtype to treat. CoA synthase (CoAsy) is a bifunctional enzyme, encoded by the COASY gene, which catalyzes the last two steps of CoA biosynthesis. COASY has been reported as a hit in several large RNAi library screens for cancer. Therefore, we sought to investigate the dependency of TNBC cell line proliferation on CoAsy expressi...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Kharabsheh HA, Scott JE Tags: Biochem Biophys Res Commun Source Type: research

Preclinical evidence that MNK/eIF4E inhibition by cercosporamide enhances the response to antiangiogenic TKI and mTOR inhibitor in renal cell carcinoma.
In this study, we investigated the anti-RCC potential of a unique Mnk inhibitor cercosporamide. We showed that cercosporamide is active against RCC cells via suppressing growth, survival and migration. Combination indices value indicated that the combination of cercosporamide with sunitinib or temsirolimus are synergistic in RCC. In two independent RCC xenograft mouse models, complete tumor growth arrest or reverse was observed throughout the duration of drug treatment in the combination of cercosporamide with sunitinib or temsirolimus groups. Of note, cercosporamide inhibited RCC angiogenesis via negatively regulating a n...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Chen S, Cui L, Hu Q, Shen Y, Jiang Y, Zhao J Tags: Biochem Biophys Res Commun Source Type: research

Ribosomal protein S3-derived repair domain peptides regulate UV-induced matrix metalloproteinase-1.
Abstract Ultraviolet (UV) radiation is a major factor that causes wrinkle formation by affecting the collagen level in the skin. Here, we show that a short peptide (A8) derived from the repair domain of the ribosomal protein S3 (rpS3) reduces UV irradiation-induced increase in matrix metalloproteinase-1 (MMP-1) and prevents collagen degradation by reducing the activation of the mitogen-activated protein kinase (MAPK) signaling proteins (extracellular signal-regulated kinase [ERK], p38, and c-Jun N-terminal kinases [JNK]) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in cells. Fur...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Yang HW, Jung Y, Kim HD, Kim J Tags: Biochem Biophys Res Commun Source Type: research

Curcumin protects cardiomyopathy damage through inhibiting the production of reactive oxygen species in type 2 diabetic mice.
Abstract Type 2 diabetes mellitus (DM)-induced cardiomyopathy is a multifactorial and complex disease involving oxidative stress, lipids, and fibrosis. It is based on metabolic disorders and microvascular disease and causes extensive focal necrosis of the heart muscle. Curcumin (CUR) is a natural polyphenol isolated from turmeric rhizomes and plays an important role in the antioxidant, anti-apoptotic and anti-inflammatory effects of diabetes. Therefore, we established a mouse model of diabetic cardiomyopathy (DCM) in type 2 diabetic db/db mice in our study. We divided the experiment into three groups: the control ...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Wu X, Huang L, Zhou X, Liu J Tags: Biochem Biophys Res Commun Source Type: research

Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo- β-N-acetylglucosaminidase.
Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase. Biochem Biophys Res Commun. 2020 Sep 10;530(1):155-159 Authors: Ohashi T, Fujisawa Y, Hayes MR, Misaki R, Pietruszka J, Fujiyama K Abstract Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties. Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired. An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, w...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Ohashi T, Fujisawa Y, Hayes MR, Misaki R, Pietruszka J, Fujiyama K Tags: Biochem Biophys Res Commun Source Type: research

Rosiglitazone binds to RXR α to induce RXRα tetramerization and NB4 cell differentiation.
In this study, we defined that rosiglitazone was also a ligand of retinoid X receptor alpha (RXRα) and displayed RXRα-dependent activities. We found that rosiglitazone directly bound to the ligand binding domain (LBD) of RXRα and induced RXRα/LBD tetramerization. Rosiglitazone inhibited the agonist-induced transcriptional activity of RXRα homodimers and heterodimers likely through inhibiting RXRα homo- and hetero-dimerization. In acute promyelocytic leukemia (APL) NB4 cells, rosiglitazone inhibited cell proliferation and induced cell differentiation, resulting from inhibiting RXRα/...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Huang F, Li Y, Chen J, Zhang XK, Zhou H Tags: Biochem Biophys Res Commun Source Type: research

The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes.
Abstract It is hard to explain the decrease in mechanosensitivity of osteocytes under microgravity. Primary cilia are essential mechanosensor for osteocytes. The cilia become shorter under the simulated microgravity (SMG) environment. The cilia change may be the reason for the mechanosensitivity decrease of osteocytes under SMG. To reveal the role of primary cilia in weightless-induced osteocyte dysfunction, we investigate intraflagellar transport (IFT) to understand the mechanism of the decreased cilia length of osteocytes when subjected to SMG. We measure the number of anterograde IFT particles with GFP::IFT88 a...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Ding D, Yang X, Luan HQ, Wu XT, Sun LW, Fan YB Tags: Biochem Biophys Res Commun Source Type: research

Combined therapy of adipose-derived stem cells and photobiomodulation on accelerated bone healing of a critical size defect in an osteoporotic rat model.
Abstract We investigated the impact of human demineralized bone matrix (hDBM) plus adipose-derived stem cells (hADS) plus photobiomodulation (PBM) on a critical-sized femoral defect (CSFD) in ovariectomy induced osteoporosis in rats. There were 6 groups as follows. In group 1 (control, C), only CSFDs were created. Groups 2-6 were implanted with DBM into the CSFD (DBM-CSFD). In group 2 (S), only DBM was transplanted into the CSFD. In group 3 (S + PBM), the DBM-CSFDs were treated with PBM. In group 4, the DBM-CSFDs were treated with alendronate (S + ALN). In group 5, ADSs were seeded into DBM-CSF...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Asgari M, Gazor R, Abdollahifar MA, Fadaei Fathabady F, Zare F, Norouzian M, Amini A, Khosravipour A, Kiani P, Atashgah RB, Rezaei F, Ghoreishi SK, Chien S, Hamblin MR, Bayat M Tags: Biochem Biophys Res Commun Source Type: research

Cytoplasmic recruitment of Mdm2 as a common characteristic of G protein-coupled receptors that undergo desensitization.
This study suggests that the property of a receptor that causes a change in subcellular localization of Mdm2, from the nuclear to cytoplasmic, could be used as a biomarker to predict the desensitization of a receptor. PMID: 32828283 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Zheng M, Zhang X, Min X, Sun N, Kim KM Tags: Biochem Biophys Res Commun Source Type: research

Protective role of mesenchymal stem cells transfected with miRNA-378a-5p in phosgene inhalation lung injury.
In conclusion, MSCs transfected with miRNA-378a-5p were more effective in treating phosgene-induced lung injury by repairing the secretion of alveolar epithelial cells and improving the permeability of vascular endothelial cells compared with MSCs alone. PMID: 32828284 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Qu Y, Zhang L, He D, Xu N, Tang Y, Shao Y, Shen J Tags: Biochem Biophys Res Commun Source Type: research

A potential mechanism underlying U1 snRNP inhibition of the cleavage step of mRNA 3' processing.
Abstract It is well established that U1 snRNP inhibits the cleavage of cryptic polyadenylation site (PAS) within introns, thereby facilitating full-length mRNA transcription for numerous genes in vertebrate cells, yet the underlying mechanism remains poorly understood. Here, by using a model PAS of wdr26 mRNA, we show that U1 snRNP predominantly interferes with the association of PAS with a core 3' processing factor CstF64, which can promote the cleavage step of mRNA 3' processing. Furthermore, we provide evidence that U1A, a component of U1 snRNP, might directly interfere with CstF64 binding on PAS through its RN...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Deng Y, Shi J, Ran Y, Xiang AP, Yao C Tags: Biochem Biophys Res Commun Source Type: research

Crystal structure of bacterial L-arabinose 1-dehydrogenase in complex with L-arabinose and NADP.
Abstract L-Arabinose 1-dehydrogenase (AraDH) is responsible for the first step of the non-phosphorylative L-arabinose pathway from bacteria, and catalyzes the NAD(P)+-dependent oxidation of L-arabinose to L-arabinonolactone. This enzyme belongs to the so-called Gfo/Idh/MocA protein superfamily, but has a very poor phylogenetic relationship with other functional members. We previously reported the crystal structures of AraDH without a ligand and in complex with NADP+. To clarify the underlying catalytic mechanisms in more detail, we herein elucidated the crystal structure in complex with L-arabinose and NADP+. In a...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Yoshiwara K, Watanabe S, Watanabe Y Tags: Biochem Biophys Res Commun Source Type: research

MicroRNAs are indispensable for the proliferation and differentiation of adult neural progenitor cells in mice.
a W Abstract More than two decades after the discovery of adult neurogenesis in humans, researchers still struggle to elucidate the underlying transcriptional and post-transcriptional mechanisms. RNA interference is a crucially important process in the central nervous system, and its role in adult neurogenesis is poorly understood. In this work, we address the role of Dicer-dependent microRNA biogenesis in neuronal differentiation of adult neural stem cells within the subventricular zone of the mouse brain. Loss of the Dicer1 gene in the tailless (Tlx)-positive cells did not cause the decline in their numbers, but...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Xu Y, Hajdukiewicz K, Tiwari A, Przybyś J, Parkitna JR, Novak M, Vinnikov IA, Schütz G, Konopka W Tags: Biochem Biophys Res Commun Source Type: research

Apo structure of the transcriptional regulator PadR from Bacillus subtilis: Structural dynamics and conserved Y70 residue.
Abstract PadR is a bacterial transcriptional regulator that controls the expression of phenolic acid decarboxylase (PadC) in response to phenolic acids to prevent their toxic effects. During transcriptional repression, PadR associates with the operator sequence at the promoter site of the padC gene. However, when phenolic acids are present, PadR directly binds the phenolic acids and undergoes an interdomain rearrangement to dissociate from the operator DNA. To further examine the structural dynamics of PadR, we determined the apo structure of Bacillus subtilis PadR. Apo-PadR exhibits significant interdomain flexib...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Park SC, Song WS, Yoon SI Tags: Biochem Biophys Res Commun Source Type: research

AgsA oligomer acts as a functional unit.
In this study, different N-terminal and C-terminal deletion mutants of AgsA were constructed and their effects on AgsA oligomer assembly and chaperone activity were investigated. We found that the IXI motif at the C-terminus and the α-helix at the N-terminus affected the oligomerization and molecular chaperone activity of AgsA. In this work, we obtained a 6.8 Å resolution structure of AgsA using Electron cryo-microscopy (cryo-EM), and found that the functional form of AgsA was an 18-mer with D3 symmetry. Through amino acid mutations, disulfide bonds were introduced into two oligomeric interfaces, namely di...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Liu D, Chen Q, Zhang L, Hu H, Yin C Tags: Biochem Biophys Res Commun Source Type: research

Integrative protocols for an in  vitro generation of pancreatic progenitors from human dental pulp stem cells.
Integrative protocols for an in vitro generation of pancreatic progenitors from human dental pulp stem cells. Biochem Biophys Res Commun. 2020 Sep 10;530(1):222-229 Authors: Sawangmake C, Rodprasert W, Osathanon T, Pavasant P Abstract Efficiency of the induction protocol is crucial for the generation of insulin-producing cells (IPCs) from human dental pulp stem cells (hDPSCs). Here, we established the integrative induction protocol by merging genetic manipulation technique with our previous published 3-step induction protocol aiming to enhance the pancreatic progenitor commitment and production y...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Sawangmake C, Rodprasert W, Osathanon T, Pavasant P Tags: Biochem Biophys Res Commun Source Type: research

Effect of TNIK upregulation on JQ1-resistant human colorectal cancer HCT116  cells.
Effect of TNIK upregulation on JQ1-resistant human colorectal cancer HCT116 cells. Biochem Biophys Res Commun. 2020 Sep 10;530(1):230-234 Authors: Takahashi C, Kondo S, Sadaoka K, Ishizuka S, Noguchi K, Kato Y, Sugimoto Y Abstract JQ1 disrupts the binding of bromodomain and extra-terminal (BET) family of proteins to acetylated histones, modulates the expression of various genes, and inhibits the proliferation of cancer cells. We established two JQ1-resistant sublines from human colorectal cancer HCT116 cells. These resistant cells showed an 8- to 9-fold higher resistance to JQ1, and a 2- to ...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Takahashi C, Kondo S, Sadaoka K, Ishizuka S, Noguchi K, Kato Y, Sugimoto Y Tags: Biochem Biophys Res Commun Source Type: research

RNA N6-methyladenosine demethylase FTO regulates PD-L1 expression in colon cancer cells.
We examined the relevance of FTO to programmed cell death-ligand 1 (PD-L1) expression in colon cancer cells. HCT-116 cells showed high expression of both FTO and PD-L1 proteins. The knockdown of FTO by small interfering RNA decreased mRNA and protein levels of PD-L1 in HCT-116 cells. To elucidate the underlying mechanism by which FTO regulates the expression of PD-L1, we depleted FTO in HCT-116 in the presence of IFN-γ, which is a major stimulus to upregulate PD-L1 expression. Depletion of FTO reduced PD-L1 expression in an IFN-γ signaling-independent manner. RNA immunoprecipitation assay revealed the...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Tsuruta N, Tsuchihashi K, Ohmura H, Yamaguchi K, Ito M, Ariyama H, Kusaba H, Akashi K, Baba E Tags: Biochem Biophys Res Commun Source Type: research

The effects of membrane potential and extracellular matrix composition on vascular differentiation of cardiac progenitor cells.
Abstract Historically, the field of tissue engineering has been adept at modulating the chemical and physical microenvironment. This approach has yielded significant progress, but it is imperative to further integrate our understanding of other fundamental cell signaling paradigms into tissue engineering methods. Bioelectric signaling has been demonstrated to be a vital part of tissue development, regeneration, and function across organ systems and the extracellular matrix is known to alter the bioelectric properties of cells. Thus, there is a need to bolster our understanding of how matrix and bioelectric signals...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Daley MC, Bonzanni M, MacKenzie AM, Kaplan DL, Black LD Tags: Biochem Biophys Res Commun Source Type: research

Synthetic protease-activated class B GPCRs.
Abstract G-protein coupled receptors (GPCRs) are the ligand detection machinery of a majority of extracellular signaling systems in metazoans. Novel chemical and biological tools to probe the structure-function relationships of GPCRs have impacted both basic and applied GPCR research. To better understand the structure-function of class B GPCRs, we generated receptor-ligand fusion chimeric proteins that can be activated by exogenous enzyme application. As a prototype, fusion proteins of the glucagon-like peptide-1 receptor (GLP-1R) with GLP-1(7-36) and exendin-4(1-39) peptides incorporating enterokinase-cleavable ...
Source: Biochemical and Biophysical Research communications - August 24, 2020 Category: Biochemistry Authors: Willard FS, Meredith TD, Showalter AD, Ma W, Ho JD, Sauder JM, Sloop KW Tags: Biochem Biophys Res Commun Source Type: research