ATG5 regulates mesenchymal stem cells differentiation and mediates chemosensitivity in acute myeloid leukemia.
Abstract Autophagy is related to the development of several tumors including acute myeloid leukemia (AML). Inhibition of autophagy in AML cells can make them more susceptible to chemotherapy. However, the influence of autophagy in mesenchymal stem cells (MSCs) remains inconclusive. In the present study, we demonstrated that the expression of ATG5 and autophagy were elevated in MSCs derived from AML patients (AML-MSCs) compared to healthy donors (HD-MSCs). After inhibiting autophagy by 3-Methyladenine (3 MA) or silencing ATG5, the differentiation potential of AML-MSCs was decreased, the fraction of G0/G1 phase...
Source: Biochemical and Biophysical Research communications - February 22, 2020 Category: Biochemistry Authors: Li Y, Jiang Y, Cheng J, Ma J, Li Q, Pang T Tags: Biochem Biophys Res Commun Source Type: research

Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds.
Abstract Collective cell migration is a key process during the development of multicellular organisms, in which the migrations of individual cells are coordinated through chemical guidance and physical contact between cells. Talin has been implicated in mechanical linkage between actin-based motile machinery and adhesion molecules, but how talin contributes to collective cell migration is unclear. Here we show that talin B is involved in chemical coordination between cells for collective cell migration at the multicellular mound stage in the development of Dictyostelium discoideum. From early aggregation to the mo...
Source: Biochemical and Biophysical Research communications - February 22, 2020 Category: Biochemistry Authors: Yamazaki SI, Hashimura H, Morimoto YV, Miyanaga Y, Matsuoka S, Kamimura Y, Ueda M Tags: Biochem Biophys Res Commun Source Type: research

Treatment of Yersinia similis with the cationic lipid DOTAP enhances adhesion to and invasion into intestinal epithelial cells - A proof-of-principle study.
This study tested the applicability of DOTAP for the enhancement of adhesion and invasion frequencies of Yersinia (Y.) similis to enable the analysis of the effects of low-pathogenic bacteria on intestinal epithelial cells. Incubation of Y. similis with DOTAP ahead of infection of C2BBe1 intestinal epithelial cells increased invasion and adhesion frequency four- and five-fold, respectively, in plating assays. Proteomic approaches confirmed the increased bacterial load on infected cells: analysis of protein extracts by two-dimensional difference gel electrophoresis (2D-DIGE) revealed higher amounts of bacterial protein...
Source: Biochemical and Biophysical Research communications - February 22, 2020 Category: Biochemistry Authors: Böhringer M, Murugaiyan J, Eravci M, Weise C, Roesler U, Neubauer H, Sprague LD Tags: Biochem Biophys Res Commun Source Type: research

Crystal structure of the M ěnglà virus VP30 C-terminal domain.
This study provides a preliminary model for investigation of MLAV VP30 CTD as an anti-filovirus drug development target. PMID: 32093889 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 22, 2020 Category: Biochemistry Authors: Dong S, Wen K, Chu H, Li H, Yu Q, Wang C, Qin X Tags: Biochem Biophys Res Commun Source Type: research

RapaLink-1 plays an antithrombotic role in antiphospholipid syndrome by improving autophagy both in  vivo and vitro.
CONCLUSION: Our results revealed that Rapalink-1 has a potential to inhibit the formation of thrombus plaque in APS and these effects were dependent on facilitating cell autophagy both in vivo and in vitro. PMID: 32093890 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 22, 2020 Category: Biochemistry Authors: Mu F, Jiang Y, Ao F, Wu H, You Q, Chen Z Tags: Biochem Biophys Res Commun Source Type: research

Melatonin suppresses ischemia-induced fibrosis by regulating miR-149.
Abstract Ischemic injury is a major risk factor for fibrosis. However, the precise mechanisms by which fibrosis is regulated and induced under ischemic oxidative stress conditions are unknown. To address this, we investigated the effect of melatonin on ischemia-induced fibrosis. In a hindlimb ischemia mouse model, ischemia induced fibrosis by increasing inflammation and the expression of extracellular matrix (ECM) proteins. Melatonin prevented ischemia-induced fibrosis in the injured tissues. In particular, melatonin suppressed the fibrosis-mediated inflammatory reaction in myoblasts through the microRNA-149 (miR-...
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Han YS, Lee JH, Lee SH Tags: Biochem Biophys Res Commun Source Type: research

Down-regulation of IDH2 sensitizes cancer cells to erastin-induced ferroptosis.
This study supports that IDH2 has protective effect against ferroptotic cell death, and that the enzyme could be targeted to sensitize cancer cells to ferroptosis. PMID: 32089268 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Kim H, Lee JH, Park JW Tags: Biochem Biophys Res Commun Source Type: research

Hetero-oligomer formation of mouse UDP-glucuronosyltransferase (UGT) 2b1 and 1a1 results in the gain of glucuronidation activity towards morphine, an activity which is absent in homo-oligomers of either UGT.
Abstract UDP-Glucuronosyltransferase (UGT, Ugt) is a major drug metabolizing enzyme family involved in the glucuronidation and subsequent elimination of drugs and small lipophilic molecules. UGT forms homo- and hetero-oligomers that enhance or suppress UGT activity. In our previous study, we characterized mouse Ugt1a1 and all the Ugt isoform belonging to the Ugt2b subfamily and revealed that mouse Ugt2b1 and Ugt1a1 cannot metabolize morphine. Mouse Ugt2b1 had been believed to function similarly to rat UGT2B1, which plays a major role in morphine glucuronidation in rat liver. Thus, in this study, we hypothesized th...
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Miyauchi Y, Kurita A, Yamashita R, Takamatsu T, Ikushiro S, Mackenzie PI, Tanaka Y, Ishii Y Tags: Biochem Biophys Res Commun Source Type: research

Spexin as an anxiety regulator in mouse hippocampus: Mechanisms for transcriptional regulation of spexin gene expression by corticotropin releasing factor.
Abstract Spexin (SPX) acts as a neuropeptide with pleiotropic functions that can participate in anxiety regulation. Corticotropin releasing factor (CRF) is widely expressed in brain tissues and associated with depression and anxiety and addiction. With the anxious mice under chronic unpredictable stress, we found SPX mRNA expression level in the hippocampus of the brain was significantly reduced, while local CRF mRNA expression level was increased. Furthermore, CRF injection in the hippocampus could also decrease SPX mRNA expression levels in hippocampus and other brain tissues, including pituitary and hypothalamu...
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Zhuang M, Lai Q, Yang C, Ma Y, Fan B, Bian Z, Lin C, Bai J, Zeng G Tags: Biochem Biophys Res Commun Source Type: research

Keratinocytes-derived exosomal miRNA regulates osteoclast differentiation in middle ear cholesteatoma.
In conclusions, we provide evidence that exosomes miRNA-17 secreted by keratinocytes in patients with middle ear cholesteatoma can up-regulate the expression of RANKL in fibroblasts and induce osteoclast differentiation. PMID: 32093888 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Gong N, Zhu W, Xu R, Teng Z, Deng C, Zhou H, Xia M, Zhao M Tags: Biochem Biophys Res Commun Source Type: research

Oleic acid up-regulates myosin heavy chain (MyHC) 1 expression and increases mitochondrial mass and maximum respiration in C2C12 myoblasts.
In this study, we examined the effects of oleic acid on the muscle fiber type of C2C12 myotubes and its relationship with PPARδ. Our results showed that oleic acid treatment increased the levels of myosin heavy chain (MyHC)1, a known type 1 fiber marker, as well as mitochondrial mass and maximum respiration in C2C12 cells. To confirm the relationship between PPARδ activation and oleic acid-induced MyHC1 increase, we examined the effects of oleic acid in PPARδ knockdown C2C12 myoblasts. We found that oleic acid supplementation increased the mRNA expression of MyHC1 in PPARδ-knockdown C2C12 ...
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: Watanabe N, Komiya Y, Sato Y, Watanabe Y, Suzuki T, Arihara K Tags: Biochem Biophys Res Commun Source Type: research

Advanced glycation end products enhance M1 macrophage polarization by activating the MAPK pathway.
CONCLUSION: AGEs enhance the expression of proinflammatory molecules by activating the MAPK pathway. Moreover, these data imply that AGEs induce macrophage M1 phenotype polarization but restrain M2 polarization, which might contribute to β-cell dysfunction in the pathogenesis of T2DM. PMID: 32093892 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 21, 2020 Category: Biochemistry Authors: He S, Hu Q, Xu X, Niu Y, Chen Y, Lu Y, Su Q, Qin L Tags: Biochem Biophys Res Commun Source Type: research

Discovery of isopenicin A, a meroterpenoid as a novel inhibitor of tubulin polymerization.
Abstract Microtubules are involved in celluar processes of movement, intracellular trafficking and mitosis, thus microtubule-targeting agents have been widely used in cancer therapy. Herein, we report isopenicin A, a novel meroterpenoid isolated from the plant endophytic fungus of Penicillium sp. sh18, as a novel microtubule binding molecule that efficiently depolymerizes microtubule polymerization to evoke G2/M cell cycle arrest and subsequent cell apoptosis, contributing to proliferation inhibition of human tumor cell lines. The discovery of isopenicin A provides a new chemotype for discovery and development of ...
Source: Biochemical and Biophysical Research communications - February 20, 2020 Category: Biochemistry Authors: Chen L, Fan DM, Tang JW, An T, Li X, Kong LM, Puno PT, Li Y Tags: Biochem Biophys Res Commun Source Type: research

Protective role of podocyte autophagy against glomerular endothelial dysfunction in diabetes.
In conclusion, podocyte autophagy plays a renoprotective role against diabetes-related structural endothelial damage, providing an additional insight into the pathogenesis of massive proteinuria in diabetic nephropathy. PMID: 32089264 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 20, 2020 Category: Biochemistry Authors: Yoshibayashi M, Kume S, Yasuda-Yamahara M, Yamahara K, Takeda N, Osawa N, Chin-Kanasaki M, Nakae Y, Yokoi H, Mukoyama M, Asanuma K, Maegawa H, Araki SI Tags: Biochem Biophys Res Commun Source Type: research

Aldehyde dehydrogenase 2 protects cardiomyocytes against lipotoxicity via the AKT/glycogen synthase kinase 3 beta pathways.
Abstract Aldehyde dehydrogenase 2, a mitochondrial matrix enzyme, plays a crucial role in protecting the heart against stress, such as ischemia reperfusion and alcohol injury. The present study aimed to investigate the effect of aldehyde dehydrogenase 2 on lipotoxic cardiomyopathy and to explore the possible mechanisms in vitro. Primary cardiomyocytes in the lipotoxic group were treated with oxidatively modified low-density lipoprotein (50 mg/L) for 24 h. Overexpression of aldehyde dehydrogenase 2 was achieved using the aldehyde dehydrogenase 2 activator, Alda-1 (20 μM). We found that cardio...
Source: Biochemical and Biophysical Research communications - February 20, 2020 Category: Biochemistry Authors: Zhao L, Fu K, Li X, Zhang R, Wang W, Xu F, Ji X, Chen Y, Li C Tags: Biochem Biophys Res Commun Source Type: research

From transglutaminases (TGs) to arylamine N-acetyltransferases (NATs): Insight into the role of a spatially conserved aromatic amino acid position in the active site of these two families of enzymes.
Abstract Transglutaminases (TG) and arylamine N-acetyltransferases (NAT) are important family of enzymes. Although they catalyze different reactions and have distinct structures, these two families of enzymes share a spatially conserved catalytic triad (Cys, His, Asp residues). In active TGs, a conserved Trp residue located close to the triad cysteine is crucial for catalysis through stabilization of transition states. Here, we show that in addition to sharing a similar catalytic triad with TGs, functional NAT enzymes also possess in their active site an aromatic residue (Phe, Tyr or Trp) occupying a structural po...
Source: Biochemical and Biophysical Research communications - February 20, 2020 Category: Biochemistry Authors: Xu X, Zhang W, Berthelet J, Liu R, Michail C, Chaffotte AF, Dupret JM, Rodrigues-Lima F Tags: Biochem Biophys Res Commun Source Type: research

Communication involvement of caveolin-1-mediated transcytosis in the intratumoral accumulation of liposomes.
Abstract For achieving efficient cancer treatment, it is important to elucidate the mechanism responsible for the accumulation of nanoparticles in tumor tissue. Recent studies suggest that nanoparticles are not delivered merely through gaps between tumor endothelial cells. We previously reported that the maturation of the vascular structure by the vascular endothelial cell growth factor receptor 2 (VEGFR2) using a previously developed siRNA delivery technology (RGD-MEND) significantly enhanced the accumulation of nanoparticles in types of cancers that area vessel-rich (renal cell carcinoma). This result was comple...
Source: Biochemical and Biophysical Research communications - February 20, 2020 Category: Biochemistry Authors: Sakurai Y, Kato A, Harashima H Tags: Biochem Biophys Res Commun Source Type: research

Negative auto-regulation of sulfur dioxide generation in vascular endothelial cells: AAT1 S-sulfenylation.
Abstract Recently, endogenous sulfur dioxide (SO2) has been found to exert an important function in the cardiovascular system. However, the regulatory mechanism for SO2 generation has not been entirely clarified. Hence, we aimed to explore the possible auto-regulation of endogenous SO2 generation and its mechanisms in vascular endothelial cells. We showed that SO2 did not affect the protein expression of aspartate aminotransferase 1 (AAT1), a major SO2 synthesis enzyme, but significantly inhibited AAT activity in primary human umbilical vein endothelial cells (HUVECs) and porcine purified AAT1 protein. An AAT1 enz...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Song Y, Peng H, Bu D, Ding X, Yang F, Zhu Z, Tian X, Zhang L, Wang X, Tang C, Huang Y, Du J, Jin H Tags: Biochem Biophys Res Commun Source Type: research

pH-sensitive CuS@Cu2S@Au nanoparticles as a drug delivery system for the chemotherapy against colon cancer.
Abstract pH-sensitive CuS@Cu2S@Au nanoparticles (NPs) are successfully prepared by sacrificing template method. The NPs are of hollow structure, which is certified by transmission electron microscopy (TEM). The CuS@Cu2S@Au NPs can be used as carriers for doxorubicin (DOX). The DOX loaded NPs exhibit pH-sensitive release of the drug, which are prospective as controllable drug delivery system. Besides, relative to the high toxicity and lethality of the free DOX, the time-dependent release of drug from the NPs is more suitable for the long-time and lasting treatment for colon cancer. The CuS@Cu2S@Au NPs with good bio...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Yan E, Zhang Z Tags: Biochem Biophys Res Commun Source Type: research

Initial conditioning and re-conditioning recruit different populations of 'fear neurons' in the basal amygdala of rats.
Abstract 'Fear neurons' in the basal amygdala (Ba) acquire excitatory responsiveness to conditioned stimuli (CS) after fear conditioning and are believed to encode aversive valence of conditioned fear. However, it is unclear whether identical fear conditioning sessions given at different times engage the same population of 'fear neurons'. Here, we recorded electrical activity from single neurons in the Ba while the same fear conditioning paradigm was performed at two different times. Conditioned fear was monitored during CS presentation after each conditioning session in order to identify 'fear neurons'. Surprisin...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Park S, Choi S Tags: Biochem Biophys Res Commun Source Type: research

Probing the effect of matrix stiffness in endocytic signalling pathway of corneal epithelium.
Abstract Matrix stiffness regulates the physiology of the cells and plays an important role in maintaining its homeostasis. It has been reported to regulate cell division, proliferation, migration, extracellular uptake and various other physiological processes. The alteration in matrix stiffness has also been well reported in various disease pathologies. However, in ocular system, Keratoconus (KC) is an ideal model to study the effect of matrix stiffness on endocytosis since the progression of the disease is controlled by increasing the stromal elasticity. Our study using corneal epithelial and retinal pigment epi...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Amit C, Padmanabhan P, Elchuri SV, Narayanan J Tags: Biochem Biophys Res Commun Source Type: research

A novel mechanism of RyeA/SraC induction under acid stress.
Abstract RyeA/SraC is a cis-encoded small RNA (sRNA), which act as an anti-toxin to RpoS-regulated RyeB toxin in Escherichia coli. Ectopic expression of RyeA was reported to diminish the RyeB accumulation by serving as a RNA trap. Lower abundance of RyeA in the early exponential growth phase turned out to be the outcome of its degradation by RNase BN/Z. In the current study, we show that RyeA is an acid stress inducible sRNA, and global stress responsive factor RpoS appeared to be inessential in RyeA induction. Although, ryeB-pphA dicistronic transcript at low pH condition was stimulated by ∼4-fold, however, R...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Gupta AK, Siddiqui N, Dutta T Tags: Biochem Biophys Res Commun Source Type: research

Lipin1 mediates cognitive impairment in fld mice via PKD-ERK pathway.
Abstract Lipin1 is important in lipid synthesis because of its phosphatidate phosphatase activity, and it also functions as transcriptional coactivators to regulate the expression of genes involved in lipid metabolism. We found that fld mice exhibit cognitive impairment, and it is related to the DAG-PKD-ERK pathway. We used fld mice to explore the relationship between lipin1 and cognitive function. Our results confirmed the presence of cognitive impairment in the hippocampus of lipin1-deficient mice. As shown in behavioral test, the spatial learning and memory ability of fld mice was much worse than that of wild-t...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Shang P, Zheng F, Han F, Song Y, Pan Z, Yu S, Zhuang X, Chen S Tags: Biochem Biophys Res Commun Source Type: research

Corrigendum to "Three-dimensional spheroids of mesenchymal stem/stromal cells promote osteogenesis by activating stemness and Wnt/ β-catenin".
Corrigendum to "Three-dimensional spheroids of mesenchymal stem/stromal cells promote osteogenesis by activating stemness and Wnt/β-catenin". Biochem Biophys Res Commun. 2020 Feb 19;: Authors: Imamura A, Kajiya H, Fujisaki S, Maeshiba M, Yanagi T, Kojima H, Ohno J PMID: 32087967 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Imamura A, Kajiya H, Fujisaki S, Maeshiba M, Yanagi T, Kojima H, Ohno J Tags: Biochem Biophys Res Commun Source Type: research

Insufficient efferocytosis by M2-like macrophages as a possible mechanism of neuropathic pain induced by nerve injury.
In this study, we show that M2-like macrophages accumulate in injured nerves to participate in the clearance of dead or dying cells (i.e., efferocytosis). Because MerTK (a receptor of dead or dying cells) levels on the surface of macrophages are limited, it seems to induce the insufficient of efferocytosis, such that the levels of dead or dying cells cannot be controlled in injured nerves. Given that efferocytosis is pivotal for resolution of inflammation, our data suggest that insufficient efferocytosis is a contributing factor in the development of chronic inflammation in injured nerves. PMID: 32087968 [PubMed - as ...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Kobayashi D, Kiguchi N, Saika F, Kishioka S, Matsuzaki S Tags: Biochem Biophys Res Commun Source Type: research

Adenosine A2A receptor activation prevents DOCA-salt induced hypertensive cardiac remodeling via iBAT.
Abstract Hypertensive cardiac remodeling is a constellation of abnormalities that includes cardiomyocyte hypertrophy and death and tissue fibrosis. Adenosine is a long-known vasodilator, through interacting with its four cell surface receptor subtypes in cardiovascular system. However, it is unclear that whether adenosine A2A receptor (A2AR) activation is involved in the cardiac remodeling in hypertension. WT mice were utilized to induce DOCA-salt sensitive hypertension and received A2AR agonist CGS21680 or antagonist KW6002 treatment. Cardiac functional phenotyping measurement by echocardiography showed that CGS2...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Zhou YP, Ruan CC, Kong LR, Gao PJ Tags: Biochem Biophys Res Commun Source Type: research

An RNA-binding-protein, NONO governs energy metabolism by regulating NAMPT in lung cancer.
Abstract The RNA binding proteins (RBPs) have multiple roles in human cancer. However, their molecular target and function have not been clearly identified. Our genomic analysis derived from patients reveals that NONO is a potential oncogenic gene in lung cancer. NONO is highly expressed in lung cancer tissues compared with normal tissues, and its expression has been correlated with the prognosis of lung cancer patients. We found that NONO significantly influences cancer cell proliferation in lung cancer. Gene expression profiles with NONO-depleted cells revealed that the sirtuin signaling pathway is highly correl...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Kim SJ, Ju JS, Park SS, Suh YA, Yoo HJ, Choi EK, Seong-Yun-Jeong, Shin Y, Park YY Tags: Biochem Biophys Res Commun Source Type: research

Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer.
Abstract Berberine (BBR), a natural isoquinoline alkaloid, has been shown to be a promising therapeutic agent for colorectal cancer (CRC), but the molecular mechanism remains unclear. Here, we used mass spectrometry-based label-free proteomics to explore the potential targets of BBR in CRC cells. Comprehensive proteomic profiles demonstrated that of 8051 identified proteins, 503 and 277 differentially expressed proteins (DEPs) were screened out of CACO2 and LOVO cells, respectively. 83 DEPs were overlapped and most of these were down-regulated. A pathway enrichment analysis pinpointed mitochondrial translation, re...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Tong M, Liu H, Hao J, Fan D Tags: Biochem Biophys Res Commun Source Type: research

EpCAM inhibits differentiation of human liver progenitor cells into hepatocytes in  vitro by activating Notch1 signaling.
EpCAM inhibits differentiation of human liver progenitor cells into hepatocytes in vitro by activating Notch1 signaling. Biochem Biophys Res Commun. 2020 Feb 19;: Authors: Tang D, Chen Y, Fu GB, Yuan TJ, Huang WJ, Wang ZY, Li WJ, Jiao YF, Yu WF, Yan HX Abstract In both normal turnover of the hepatic tissue and acute hepatic injury, the liver predominantly activates terminally differentiated hepatocytes to proliferate and repair. However, in chronic and severe chronic injury, this capacity fails, and liver progenitor cells (LPCs) can give rise to hepatocytes to restore both hepatic architecture an...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Tang D, Chen Y, Fu GB, Yuan TJ, Huang WJ, Wang ZY, Li WJ, Jiao YF, Yu WF, Yan HX Tags: Biochem Biophys Res Commun Source Type: research

Microglial Hv1 exacerbates secondary damage after spinal cord injury in mice.
Abstract The pathological process of spinal cord injury (SCI) is complex, particularly during secondary damage that triggers a multiphasic glial reaction consisting of both detrimental and beneficial effects. Deletion of a novel voltage-gated proton channel (Hv1) functionally expressed in microglia has been shown to confer neuroprotection during ischemic stroke. Here, we hypothesized that microglial Hv1 may also participate in the process of SCI through modulating glial responses. To test this hypothesis, we employed an SCI model in Hv1-knockout (Hv1-/-) and wild type (WT) mice and assessed resulting microglial po...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Li X, Liu R, Yu Z, He D, Zong W, Wang M, Xie M, Wang W, Luo X Tags: Biochem Biophys Res Commun Source Type: research

Phosphorylation and dephosphorylation of Ca2+/calmodulin-dependent protein kinase kinase β at Thr144 in HeLa cells.
In this report, we found that phosphorylation of CaMKKβ is dynamically regulated by protein phosphatase/kinase system in HeLa cells. Global phosphoproteomic analysis revealed the constitutive phosphorylation at 8 Ser residues including Ser128, 132, and 136 in the N-terminal regulatory domain of rat CaMKKβ in unstimulated HeLa cells as well as inducible phosphorylation of Thr144 in the cells treated with a phosphatase inhibitor, okadaic acid (OA). Thr144 phosphorylation in CaMKKβ has shown to be rapidly induced by OA treatment in a time- and dose-dependent manner in transfected HeLa cells, indicating that Thr...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Takabatake S, Fukumoto Y, Ohtsuka S, Kanayama N, Magari M, Sakagami H, Hatano N, Tokumitsu H Tags: Biochem Biophys Res Commun Source Type: research

LINC00028 regulates the development of TGF β1-treated human tenon capsule fibroblasts by targeting miR-204-5p.
LINC00028 regulates the development of TGFβ1-treated human tenon capsule fibroblasts by targeting miR-204-5p. Biochem Biophys Res Commun. 2020 Feb 19;: Authors: Sui H, Fan S, Liu W, Li Y, Zhang X, Du Y, Bao H Abstract Glaucoma is a leading cause of blindness worldwide with complex pathogenesis. The excessive proliferation and fibrosis of human tenon capsule fibroblasts (HTFs) trigger the scar formation after glaucoma filtration surgery. The purpose was to investigate the role of long intergenic non-protein coding RNA 28 (LINC00028) and mechanism in transforming growth factor β1 (TGFβ1)-...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Sui H, Fan S, Liu W, Li Y, Zhang X, Du Y, Bao H Tags: Biochem Biophys Res Commun Source Type: research

Biochemical and structural characterization of the Holliday junction resolvase RuvC from Pseudomonas aeruginosa.
Abstract The Holliday junction, a four-way DNA structure, is an important intermediate of homologous recombination. Proper Holliday junction resolution is critical to complete the recombination process. In most bacterial cells, the Holliday junction cleavage is mainly performed by a specific endonuclease RuvC. Here, we describe the biochemical properties and the crystal structure of RuvC from an opportunistic pathogen, Pseudomonas aeruginosa (PaRuvC). PaRuvC specifically binds to the Holliday junction DNA and preferentially cleaves it at the consensus 5'-TTC-3'. PaRuvC uses Mg2+ as the preferred divalent metal cof...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Hu Y, He Y, Lin Z Tags: Biochem Biophys Res Commun Source Type: research

Cellular mRNA export factor UAP56 recognizes nucleic acid binding site of influenza virus NP protein.
Abstract Influenza A virus nucleoprotein (NP) is a structural component that encapsulates the viral genome into the form of ribonucleoprotein complexes (vRNPs). Efficient assembly of vRNPs is critical for the virus life cycle. The assembly route from RNA-free NP to the NP-RNA polymer in vRNPs has been suggested to require a cellular factor UAP56, but the mechanism is poorly understood. Here, we characterized the interaction between NP and UAP56 using recombinant proteins and showed that UAP56 features two NP binding sites. In addition to the UAP56 core comprised of two RecA domains, we identified the N-terminal ex...
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Morris AK, Wang Z, Ivey AL, Xie Y, Hill PS, Schey KL, Ren Y Tags: Biochem Biophys Res Commun Source Type: research

Septin4 as an autophagy modulator regulates Angiotensin-II mediated VSMCs proliferation and migration.
In conclusion, our results indicated that during the process of VSMCs proliferation and migration induced by Ang-II, Septin4 modulated autophagy and thus regulated the activity of proliferation and migration. PMID: 32085901 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 19, 2020 Category: Biochemistry Authors: Wang N, Xu F, Lu S, Zhang N, Sun Y Tags: Biochem Biophys Res Commun Source Type: research

1 α, 25(OH)2D3 regulates agrin-induced acetylcholine receptor clustering through upregulation of rapsyn expression in C2C12 myotubes.
1α, 25(OH)2D3 regulates agrin-induced acetylcholine receptor clustering through upregulation of rapsyn expression in C2C12 myotubes. Biochem Biophys Res Commun. 2020 Feb 18;: Authors: Arakawa M, Wagatsuma A Abstract The active form of vitamin D (1α, 25-dihydroxyvitamin D3 [1α, 25(OH)2D3], referred to as 1,25D) has been suggested to play a pivotal role in skeletal muscle function and metabolism. However, the mechanisms through which 1,25D functions in this tissue remain to be elucidated. Recent studies have shown that vitamin D signaling regulates neuromuscular maintenance and improve...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Arakawa M, Wagatsuma A Tags: Biochem Biophys Res Commun Source Type: research

Glioma-derived endothelial cells promote glioma cells migration via extracellular vesicles-mediated transfer of MYO1C.
Abstract Extracellular vesicles (EV), as the intercellular information transfer molecules which can regulate the tumor microenvironment, promote migration and tumor progression. Previous studies reported that EV from endothelial cells was used to guide the fate and survival of gliomas, but many researches focus on normal human endothelial cells (NhEC) rather than tumor-derived endothelial cells. Our laboratory isolated human endothelial cells from glioma issue (GhEC). We have previously demonstrated that EV from GhEC and NhEC, which both can promote glioma stem cells (GSC) proliferation and tumorsphere formation i...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Tian Y, Wang Z, Wang Y, Yin B, Yuan J, Qiang B, Han W, Peng X Tags: Biochem Biophys Res Commun Source Type: research

Estrogen Receptor alpha depletion affects the biomechanical properties and cytoskeleton rearrangements in breast cancer cells.
In this study, we found that tamoxifen-resistant breast cancer cells (MCF-7/TamR) altered cell morphology and lacked of ERα expression during the process of the Tamoxifen resistance induction. To determine whether this change was influenced by ERα expression, we transiently constructed another ERα depleted model with ERα siRNA (MCF-7/ERα siRNA) and used atomic force microscope (AFM) to detect morphological and biophysical changes. The results indicated that the roughness and Young's modulus of ERα expression depleted cells were significantly increased, accompanied by rearrangement of the...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Zhao C, Hou X, Peng Z, Sun X, Li E, Yang H, Lu Y, Zhu L Tags: Biochem Biophys Res Commun Source Type: research

Disrupted blood-brain barrier in 5 ×FAD mouse model of Alzheimer's disease can be mimicked and repaired in vitro with neural stem cell-derived exosomes.
Disrupted blood-brain barrier in 5×FAD mouse model of Alzheimer's disease can be mimicked and repaired in vitro with neural stem cell-derived exosomes. Biochem Biophys Res Commun. 2020 Feb 18;: Authors: Liu Y, Huber CC, Wang H Abstract Alzheimer's disease (AD) is a devastating neurodegenerative disease and is associated with blood-brain barrier (BBB) disruption. AD mice and cell culture models play an essential role in understanding AD pathogenesis and validation of therapeutic reagents. One of the commonly used AD mice is the 5 × FAD mouse and previous studies have shown th...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Liu Y, Huber CC, Wang H Tags: Biochem Biophys Res Commun Source Type: research

Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA.
In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of wa...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Tateishi-Karimata H, Banerjee D, Ohyama T, Matsumoto S, Miyoshi D, Nakano SI, Sugimoto N Tags: Biochem Biophys Res Commun Source Type: research

Invasive phenotype induced by low extracellular pH requires mitochondria dependent metabolic flexibility.
Abstract Metabolic reprogramming is required for tumors to meet the bioenergetic and biosynthetic demands of malignant progression. Numerous studies have established a causal relationship between oncogenic drivers and altered metabolism, most prominently aerobic glycolysis, which supports rapid growth and affects the tumor microenvironment. Less is known about how the microenvironment modulates cancer metabolism. In the present study, we found that low extracellular pH, a common feature of solid tumors, provoked PDAC cells to decrease glycolysis and become resistant to glucose starvation. This was accompanied by i...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Shin SC, Thomas D, Radhakrishnan P, Hollingsworth MA Tags: Biochem Biophys Res Commun Source Type: research

Ubiquitination of Src promotes its secretion via small extracellular vesicles.
Abstract Upregulation of the Src tyrosine kinase is implicated in the progression of cancer. The oncogenic potential of Src is suppressed via several negative regulation systems including degradation via the ubiquitin-proteasome pathway. Here, we show that ubiquitination of Src promotes its secretion via small extracellular vesicles (sEVs) to suppress its oncogenic potential. In MDCK cells expressing a modified Src that can be activated by hydroxytamoxifen, activated Src was transported to late endosomes/lysosomes and secreted via sEVs. The secretion of Src was suppressed by ablation of Cbl E3-ligase, suggesting t...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Tanaka K, Ito Y, Kajiwara K, Nada S, Okada M Tags: Biochem Biophys Res Commun Source Type: research

Characterization of heteromeric complexes between chemokine (C-X-C motif) receptor 4 and α1-adrenergic receptors utilizing intermolecular bioluminescence resonance energy transfer assays.
Characterization of heteromeric complexes between chemokine (C-X-C motif) receptor 4 and α1-adrenergic receptors utilizing intermolecular bioluminescence resonance energy transfer assays. Biochem Biophys Res Commun. 2020 Feb 18;: Authors: Gao X, Enten GA, DeSantis AJ, Volkman BF, Gaponenko V, Majetschak M Abstract Recently, we reported that chemokine (C-X-C motif) receptor 4 (CXCR4) heteromerizes with α1-adrenergic receptors (AR) on the cell surface of vascular smooth muscle cells, through which the receptors cross-talk. Direct biophysical evidence for CXCR4:α1-AR heteromers, however...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Gao X, Enten GA, DeSantis AJ, Volkman BF, Gaponenko V, Majetschak M Tags: Biochem Biophys Res Commun Source Type: research

Unraveling the molecular mechanism of BNC105, a phase II clinical trial vascular disrupting agent, provides insights into drug design.
Abstract Microtubules are made up of tubulin protein and play a very important part in numerous cellular events of eukaryotic cells, which is why they are seen as attractive targets for tumor chemotherapy. BNC105, a known vascular targeting agent, has entered in phase II clinical trials. It has previously been confirmed that BNC105 is an effective microtubule targeting agent for various cancers. BNC105 exhibits selectivity for tumor cells, elicits vascular disrupting effects, and inhibits tumor growth. However, the molecular mechanism of BNC105 is still elusive. Herein, the crystal structure of BNC105 in complex w...
Source: Biochemical and Biophysical Research communications - February 18, 2020 Category: Biochemistry Authors: Wang T, Wu C, Wang C, Zhang G, Arnst KE, Yao Y, Zhang Z, Wang Y, Pu D, Li W Tags: Biochem Biophys Res Commun Source Type: research

Structures of human galectin-10/monosaccharide complexes demonstrate potential of monosaccharides as effectors in forming Charcot-Leyden crystals.
Abstract The galectins are a family of β-galactoside-specific animal lectins, and have attracted much attention as novel regulators of the immune system. Galectin-10 is well-expressed in eosinophils, and spontaneously forms Charcot-Leyden crystals (CLCs), during prolonged eosinophilic inflammatory reactions, which are frequently observed in eosinophilic diseases. Although biochemical and structural characterizations of galectin-10 have been done, its biological role and molecular mechanism are still unclear, and few X-ray structures of galectin-10 in complex with monosaccharides/oligosaccharides have been rep...
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Itoh A, Nonaka Y, Nakakita SI, Yoshida H, Nishi N, Nakamura T, Kamitori S Tags: Biochem Biophys Res Commun Source Type: research

Mutations of histone demethylase genes encoded by X and Y chromosomes, Kdm5c and Kdm5d, lead to noncompaction cardiomyopathy in mice.
In this study, to explore the functional conservation and divergence between these two genes in other organs, we generated female mice lacking Kdm5c (homozygous X5c- X5c- females) and male mice lacking both Kdm5c and Kdm5d (compound hemizygous X5c- Y5d- males). Both X5c- X5c- females and X5c- Y5d- males showed lower body weights and postnatal lethality. Histological examination of the hearts showed prominent trabecular extension and a thin layer of compacted myocardium in the left and right ventricles, indicating noncompaction cardiomyopathy. However, hemizygous males lacking either Kdm5c or Kdm5d showed no signs of noncom...
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Kosugi M, Otani M, Kikkawa Y, Itakura Y, Sakai K, Ito T, Toyoda M, Sekita Y, Kimura T Tags: Biochem Biophys Res Commun Source Type: research

Menthol relieves acid reflux inflammation by regulating TRPV1 in esophageal epithelial cells.
Abstract Transient receptor potential cation channel subfamily V member 1 (TRPV1) plays an important role in pain and inflammatory responses. Previous studies have shown that the expression of TRPV1 increases in the sensory neurons of the esophagus during the development of gastroesophageal reflux disease and esophagitis, but the response of TRPV1 in esophageal epithelial cells (EECs), which directly confront the refluxed acid, is still unknown. Here, we found that acid reflux triggered esophageal damage, which was accompanied by increased expression of TRPV1 in EECs and TRPV1 channel activity in these cells. Furt...
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Zhang Z, Wu X, Zhang L, Mao A, Ma X, He D Tags: Biochem Biophys Res Commun Source Type: research

Formononetin recovered injured nerve functions by enhancing synaptic plasticity in ischemic stroke rats.
CONCLUSIONS: Formononetin recovered injured nerve functions after ischemic stroke. PI3K/AKT/ERK pathway might involve in the beneficial effect of formononetin on the neuronal differentiation and synaptic plasticity. PMID: 32081422 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Wu QL, Cheng YQ, Liu AJ, Zhang WD Tags: Biochem Biophys Res Commun Source Type: research

Simultaneous tracking of two motor domains reveals near simultaneous steps and stutter steps of myosin 10 on actin filament bundles.
Abstract Myosin X (Myo10) has several unique design features including dimerization via an anti-parallel coiled coil and a long lever arm, which allow it to preferentially move on actin bundles. To understand the stepping behavior of single Myo10 on actin bundles, we labeled two heads of Myo10 dimers with different fluorophores. Unlike previously described for myosin V (Myo5) and VI (Myo6), which display alternating hand-over-hand stepping, Myo10 frequently took near simultaneous steps of both heads, and less frequently, 2-3 steps of one head before the other head stepped. We suggest that this behavior results fro...
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Qin X, Yoo H, Man Cheng HC, Nguyen QQ, Li J, Liu X, Prunetti L, Chen X, Liu T, Sweeney HL, Park H Tags: Biochem Biophys Res Commun Source Type: research

The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase.
Abstract GPI anchored proteins (GPI-APs) act at the frontiers of cells, decoding environmental cues and determining host-pathogen interactions in several lower eukaryotes. They are also essential for viability in lower eukaryotes. The GPI biosynthetic pathway begins at the ER and follows a roughly linear pathway to generate the complete precursor (CP) glycolipid. The GPI transamidase (GPIT) transfers this glycolipid to the C-terminal end of newly translated proteins after removing their GPI attachment signal sequence (SS). The GPIT subunit that cleaves SS is Gpi8, a protein with a conserved Cys/His catalytic dyad ...
Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Sah SK, Shefali S, Yadav A, Som P, Komath SS Tags: Biochem Biophys Res Commun Source Type: research