Histone demethylase KDM3B regulates the transcriptional network of cell-cycle genes in hepatocarcinoma HepG2 cells.
Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most lethal cancer worldwide. Although gene mutations associated with HCC development have been intensively studied, how epigenetic factors specifically modulate the functional properties of HCC by regulating target gene expression is unclear. Here we demonstrated the overexpression of KDM3B in liver tissue of HCC patients using public RNA-seq data. Ablation of KDM3B by CRISPR/Cas9 retarded the cell cycle and proliferation of hepatocarcinoma HepG2 cells. Approximately 30% of KDM3B knockout cells exhibited mitotic spindle multipol...
Source: Biochemical and Biophysical Research communications - December 1, 2018 Category: Biochemistry Authors: An MJ, Kim DH, Kim CH, Kim M, Rhee S, Seo SB, Kim JW Tags: Biochem Biophys Res Commun Source Type: research

miR1916 plays a role as a negative regulator in drought stress resistance in tomato and tobacco.
Abstract MicroRNAs (miRNAs) play important roles in regulating plant responses to various environmental stresses. In our study, overexpression of miR1916 in tomato (OE-1) reduced its tolerance to drought. The miR1916-silenced transgenic plants (ST-1 and Anti-7) significantly increased resistance to drought stress. The transgenic tobacco plants also have a similar result in displaying the tolerance of drought. Physiological analysis revealed that miR1916 affected the osmoregulation and reactive oxygen species (ROS) accumulation. In addition, transcript levels of the miR1916 target genes, histone deacetylases (HDAC)...
Source: Biochemical and Biophysical Research communications - December 1, 2018 Category: Biochemistry Authors: Chen L, Meng J, Luan Y Tags: Biochem Biophys Res Commun Source Type: research

5-aminolaevulinic acid (ALA), enhances heme oxygenase (HO)-1 expression and attenuates tubulointerstitial fibrosis and renal apoptosis in chronic cyclosporine nephropathy.
CONCLUSION: The activity of 5-ALA/SFC has important implications for clarifying the mechanism of HO-1 activity in CsA-induced nephrotoxicity and may provide a favorable basis for clinical therapy. PMID: 30514440 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 1, 2018 Category: Biochemistry Authors: Liu C, Zhu P, Fujino M, Isaka Y, Ito H, Takahashi K, Nakajima M, Tanaka T, Zhuang J, Li XK Tags: Biochem Biophys Res Commun Source Type: research

Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells.
CONCLUSIONS: Our study revealed the involvement of miR-218 in regulating the inflammatory response of macrophages against L. pneumophila infection. These findings suggest potential novel roles for miR-218 and RICTOR as therapeutic targets of L. pneumophila infection. PMID: 30509489 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 1, 2018 Category: Biochemistry Authors: Koriyama T, Yamakuchi M, Takenouchi K, Oyama Y, Takenaka H, Nagakura T, Masamoto I, Hashiguchi T Tags: Biochem Biophys Res Commun Source Type: research

SR 57227A is a partial agonist/partial antagonist of 5-HT3 receptor and inhibits subsequent 5-HT- or SR 57227A-induced 5-HT3 receptor current.
Abstract The serotonin (5-hydroxytryptamine) type 3 (5-HT3) receptors are transmembrane ligand-gated ion channels. Although several 5-HT3 receptor agonists have been used as preclinical tools, SR 57227A is the most commonly used 5-HT3 receptor agonist with the ability to cross the blood brain barrier. However, the precise pharmacological profile of SR 57227A remains unclear. Therefore, we examined the pharmacological profile of SR 57227A at the 5-HT3A and 5-HT3AB receptors. We microinjected Xenopus laevis oocytes with human 5-HT3A complementary RNA (cRNA) or a combination of human 5-HT3A and human 5-HT3AB cRNA and...
Source: Biochemical and Biophysical Research communications - December 1, 2018 Category: Biochemistry Authors: Nakamura Y, Kondo M, Koyama Y, Shimada S Tags: Biochem Biophys Res Commun Source Type: research

TLR5 binding and activation by KMRC011, a flagellin-derived radiation countermeasure.
Abstract Entolimod (CBLB502) is a flagellin-derived radiation countermeasure currently under clinical trial. Entolimod exerts radioprotective activity by directly interacting with TLR5, an innate immune receptor, using the conserved domains of flagellin. Entolimod was designed to contain an artificially introduced N-terminal region that is not related to drug effects and might trigger unexpected toxic immunogenic reactions in humans. To refine the entolimod drug design, we engineered entolimod into KMRC011 by removing its ancillary region. The TLR5 binding and activating capacities of KMRC011 were assessed through...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Song WS, Kim JH, Choi CM, Lee WJ, Yoon SI Tags: Biochem Biophys Res Commun Source Type: research

Immobilization stress induces XBP1 splicing in the mouse brain.
Abstract Cells activate the unfolded protein response (UPR) to cope with endoplasmic reticulum (ER) stress. In the present study, we investigated the possible involvement of psychological stress on UPR induction in the mouse brain. When mice were exposed to immobilization stress for 8 h, XBP1 mRNA splicing was significantly induced in the hippocampus, cortex, hypothalamus, cerebellum, and brain stem. On the other hand, we did not observe any increase in XBP1 splicing in the liver, suggesting that this effect is specific to the brain. Stress-induced XBP1 splicing was attenuated 2 days after immobilization stress....
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Hosoi T, Kimura H, Yamawaki Y, Mori K, Ozawa K Tags: Biochem Biophys Res Commun Source Type: research

Generation of a novel isogenic trisomy panel in human embryonic stem cells via microcell-mediated chromosome transfer.
In this study, we established isogenic human embryonic stem (hES) cells of Ts8, Ts13, and Ts18 in addition to previously established Ts21 by transferring each single chromosome into parental hES cells via microcell-mediated chromosome transfer. Genes on each trisomic chromosome were globally overexpressed in each established cell line, and all Ts cell lines differentiated into all three embryonic germ layers. This cell line panel is expected to be a useful resource to elucidate molecular and epigenetic mechanisms of genetic imbalance and determine how aneuploidy is involved in various abnormal phenotypes including tumourig...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Hiramatsu K, Abe S, Kazuki K, Osaki M, Kajitani N, Yakura Y, Oshimura M, Kazuki Y Tags: Biochem Biophys Res Commun Source Type: research

Reactive sulfur species impair Ca2+/calmodulin-dependent protein kinase II via polysulfidation.
Abstract We previously reported that Ca2+/calmodulin-dependent protein kinase II (CaMKII) is inhibited by S-nitrosylation of Cys6 in cells. Herein, we show that polysulfidation of CaMKII at Cys6 limits its enzyme activity following reactive sulfur species (RSS) stimulus. In vitro incubation of CaMKII with the RSS donor, Na2S4, induced the inhibition of the enzyme via its polysulfidation. Treatment with dithiothreitol reversed the polysulfidation and the subsequent inhibition. The inhibition of CaMKII by Na2S4 is competitive with ATP but not with the peptide substrate Syntide-2. In transfected cells expressing...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Araki S, Takata T, Tsuchiya Y, Watanabe Y Tags: Biochem Biophys Res Commun Source Type: research

Circular RNA hsa_circ_0004015 regulates the proliferation, invasion, and TKI drug resistance of non-small cell lung cancer by miR-1183/PDPK1 signaling pathway.
In conclusion, circ_0016760/miR-1183/PDPK1 signaling pathway might play vital roles in the tumorigenesis of NSCLC. PMID: 30509491 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Zhou Y, Zheng X, Xu B, Chen L, Wang Q, Deng H, Jiang J Tags: Biochem Biophys Res Commun Source Type: research

LncRNA LCPAT1 is involved in DNA damage induced by CSE.
Abstract Cigarette smoking plays an important role in the process of lung cancer, during which DNA damage is proved to be involved. Non-coding RNAs are found to be involved in the DNA damage and repair processes induced by cigarette smoke. In the present study, we investigated the role of lncRNA LCPAT1 in DNA damage caused by CSE in Beas-2B cells. Our results indicate that LCPAT1, through RCC2 is involved in the CSE-induced DNA damage providing new insight into the lung carcinogenesis related to cigarette smoking. PMID: 30509493 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Gao S, Lin H, Yu W, Zhang F, Wang R, Yu H, Qian B Tags: Biochem Biophys Res Commun Source Type: research

CDH11 promotes liver fibrosis via activation of hepatic stellate cells.
Abstract Liver fibrosis, an important health condition associated with chronic liver injury that provides a permissive environment for cancer development, is characterized by the persistent deposition of extracellular matrix components that are mainly derived from activated hepatic stellate cells (HSCs). CDH11 belongs to a group of transmembrane proteins that are principally located in adherens junctions. CDH11 mediates homophilic cell-to-cell adhesion, which may promote the development of cirrhosis. The goal of this study was to determine whether CDH11 regulates liver fibrosis and to examine its mechanism by focu...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Ruan W, Pan R, Shen X, Nie Y, Wu Y Tags: Biochem Biophys Res Commun Source Type: research

Toxicity of food sweetener-sodium cyclamate on osteoblasts cells.
Abstract In this paper, the effect of commonly used food sweetener (sodium cyclamate) on the proliferation and differentiation of osteoblasts has been researched. The morophology change of osteoblasts was investigated by confocal laser scanning microscopy. Cell viability was studied by MTT analysis. BMP2 expression was analyzed by western blot and immunofluorescence. Mineralization ability of osteoblasts was researched by using alizarin red staining method. The results indicate that a very low concentration (0.06 μM) of sodium cyclamate can curle and fold microfilament and microtubule of osteoblasts. The incr...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Chen Z, Chen G, Zhou K, Zhang P, Ren X, Mei X Tags: Biochem Biophys Res Commun Source Type: research

Integrative lipidomic and transcriptomic analysis of X-linked adrenoleukodystrophy reveals distinct lipidome signatures between adrenomyeloneuropathy and childhood cerebral adrenoleukodystrophy.
In conclusion, the homeostatic imbalance of the complex lipid networks may be pathogenically important in X-ALD and the particular dysregulations of TG and CER may further influence the severity of CCALD among X-ALD patients. PMID: 30509496 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Lee DK, Long NP, Jung J, Kim TJ, Na E, Kang YP, Kwon SW, Jang J Tags: Biochem Biophys Res Commun Source Type: research

Loss of Limb-Bud-and-Heart (LBH) attenuates mammary hyperplasia and tumor development in MMTV-Wnt1 transgenic mice.
Abstract WNT/β-catenin signaling plays pivotal roles in mammary development and tumorigenesis; and aberrant activation of this pathway is frequently observed in human breast cancer, correlating with poor outcome. However, the mechanisms underlying WNT-driven mammary tumorigenesis remain incompletely understood. Here, we used mouse mammary tumor virus (MMTV)-Wnt1 transgenic mice, which develop aggressive mammary adenocarcinomas, to examine whether Limb-Bud-and-Heart (LBH) - a WNT/β-catenin target transcription co-factor overexpressed in human triple-negative breast cancers with WNT pathway hyperactivation...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Ashad-Bishop K, Garikapati K, Lindley LE, Jorda M, Briegel KJ Tags: Biochem Biophys Res Commun Source Type: research

Identification of Rfx6 target genes involved in pancreas development and insulin translation by ChIP-seq.
Abstract Regulatory Factor X-box binding transcriptional factor 6 (Rfx6) plays an important role in the differentiation and development of pancreas in mammals. However, the direct target genes of Rfx6 to regulate this process were largely unknown. The present study aimed to investigate the function of Rfx6 on regulating pancreatic differentiation and development in a physiologically-relevant context. We performed the chromatin immunoprecipitation followed by the next generation sequencing analysis (ChIP-seq) using whole pancreatic tissue harvested from C57/BL6 adult mice to find target genes of Rfx6. We captured 4...
Source: Biochemical and Biophysical Research communications - November 30, 2018 Category: Biochemistry Authors: Cheng C, Lu J, Cao X, Yang FY, Liu JY, Song LN, Shen H, Liu C, Zhu XR, Zhou JB, Yang JK Tags: Biochem Biophys Res Commun Source Type: research

Clinically relevant concentration of anti-viral drug ribavirin selectively targets pediatric osteosarcoma and increases chemosensitivity.
Abstract Ribavirin is an anti-viral drug but has recently gained attention as a potential candidate for cancer treatment. In line with these efforts, our work is the first to demonstrate that ribavirin, at clinically relevant concentration, selectively targets pediatric osteosarcoma and increases chemosensitivity. Using preclinical osteosarcoma cell and xenograft models, we found that ribavirin is active against osteosarcoma bulk and subpopulations with highly proliferative and invasive properties via inhibiting growth, inducing apoptosis and suppressing colony formation. At the same concentrations, ribavirin eith...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Chen J, Xu X, Chen J Tags: Biochem Biophys Res Commun Source Type: research

Mesenchymal stem cells in combination with low-dose rapamycin significantly prolong islet  allograft survival through induction of regulatory T cells.
Mesenchymal stem cells in combination with low-dose rapamycin significantly prolong islet allograft survival through induction of regulatory T cells. Biochem Biophys Res Commun. 2018 Nov 30;506(3):619-625 Authors: Duan W, Yu X, Ma D, Yang B, Li Y, Huang L, Liu L, Chen G, Xu D, Ding Y Abstract We previously demonstrated the protective effect of MSCs in an adaptive transfer mouse model. However, their therapeutic potential in an allogeneic immunocompetent setting mimicking clinical context of islet transplantation remained unknown. The aim of this study was to determine whether MSCs therapy, either...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Duan W, Yu X, Ma D, Yang B, Li Y, Huang L, Liu L, Chen G, Xu D, Ding Y Tags: Biochem Biophys Res Commun Source Type: research

Pyroptosis of HUVECs can be induced by heat stroke.
Abstract Heat stroke is a serious pathological condition with high mortality. Vascular endothelial cell injury is a key feature in the pathogenesis of heat stroke, but the specific pathophysiological process whereby this occurs is still unclear. Currently, relevant studies are primarily based upon examination of apoptosis. Recently, pyroptosis, a new form of inflammation-related programmed cell death, was also demonstrated to be involved in heat stroke pathophysiology. Herein, we present evidence that vascular endothelial cell pyroptosis can be induced by heat stress in a time- and temperature-dependent manner. Fu...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Pei Y, Geng Y, Su L Tags: Biochem Biophys Res Commun Source Type: research

Lnc-SNHG1 may promote the progression of non-small cell lung cancer by acting as a sponge of miR-497.
In this study, we investigated the detailed effects and possible molecular mechanisms. The transcript level of lnc-SNHG1 was higher in lung adenocarcinoma specimens and NSCLC cell lines than in noncancer tissue and cells. The level of expression was positively correlated with invasiveness and was negatively correlated with the level of miR-497 in vivo and in vitro. In exploring the regulatory mechanism, we found that lnc-SNHG1 might modulate tumor growth by sponging miR-497. The inhibitory effect of si-lnc-SNHG1 on NSCLC cell proliferation, migration and invasion could be rescued by miR-497 inhibition, while the ...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Li Z, Lu Q, Zhu D, Han Y, Zhou X, Ren T Tags: Biochem Biophys Res Commun Source Type: research

Role of DDX53 in taxol-resistance of cervix cancer cells in  vitro.
In this study, the role of DDX53 in taxol-resistance of cervix cancer cells was investigated. In taxol-resistant HelaTR cells, DDX53 was significantly increased as compared to the parental HeLa cells. HelaTR cells also showed upregulation of multidrug resistant gene MDR1, invasive characteristics and decreased apoptosis. In addition, increased autophagy level was observed in HelaTR cells. Overexpression of DDX53 in HeLa and SiHa markedly led to greater resistance to taxol and cisplatin, whereas knockdown of DDX53 in HelaTR cells restored sensitivity, demonstrating that DDX53 regulated taxol resistance in cervix cancer cell...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Park SY, Kim WJ, Byun JH, Lee JJ, Jeoung D, Park ST, Kim Y Tags: Biochem Biophys Res Commun Source Type: research

High sensitivity of cerebellar neurons to homocysteine is determined by expression of GluN2C and GluN2D subunits of NMDA receptors.
Abstract Homocysteine (HCY) induced neurotoxicity largely depends on interaction of this endogenous amino acid with glutamate NMDA receptors (NMDARs). This receptor type is composed by GluN1 and different GluN2 (A, B, C or D) subunits. However, the receptor activity of HCY in brain regions which differ in relative contribution of GluN2 subunits was not tested so far. In the current study, we explored the action of HCY on cerebellar neurons which natively express GluN2C and GluN2D subunits of NMDARs and compared this with the action of HCY on cortical neurons which are mainly composed by GluN2A and GluN2B subunits....
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Sibarov DA, Giniatullin R, Antonov SM Tags: Biochem Biophys Res Commun Source Type: research

Rational affinity enhancement of fragmented antibody by ligand-based affinity improvement approach.
Abstract Antibody engineering is now a noteworthy area in biopharmaceuticals as the next generation of marketed antibodies is engineered antibodies such as affinity- or stability-improved antibodies, fragmented or fused antibodies, antibody drug conjugates (ADCs), and PEGylated antibody fragments. In the current study, affinity enhancement of Nb against PlGF was performed by an in silico affinity maturation and molecular dynamics (MD) simulation. First, 300 single-point mutants were designed by identifying the residues involved in interaction with PlGF and different energy distributions. An energy based screening ...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Ebrahimi Z, Asgari S, Ahangari Cohan R, Hosseinzadeh R, Hosseinzadeh G, Arezumand R Tags: Biochem Biophys Res Commun Source Type: research

Atypical Switch-I Arginine plays a catalytic role in GTP hydrolysis by Rab21 from Entamoeba histolytica.
Abstract Entamoeba histolytica, the causative agent of amoebic dysentery, liver abscess and colitis, exploits its vesicular trafficking machinery for survival and virulence. Rab family of small GTPases play a key role in the vesicular transport by undergoing the GTP/GDP cycle which is central to the biological processes. Amoebic genome encodes several atypical Rab GTPases which are unique due to absence of conserved sequence motif(s) or atypical residues in their catalytic site [Saito-Nakano et al., 2005 ]. Previously, EhRab21 has been reported to involve in amoebic invasion and migration [Emmanuel et al...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Kotyada C, Chandra M, Tripathi A, Narooka AR, Datta S, Verma A Tags: Biochem Biophys Res Commun Source Type: research

Leukocytic toll-like receptor 2 knockout protects against diabetes-induced cardiac dysfunction.
Abstract Diabetic cardiomyopathy is characterized by the deterioration of the myocardial function. Emerging evidences have indicated that leukocytic toll-like receptor 2 (TLR2) played an important role in the development of diabetic cardiomyopathy. Our study aimed to investigate whether TLR2 knockout (KO) exerted a cardioprotective effect in vivo. The establishment of diabetes model was set up in mice via intraperitoneal injection of streptozotocin (STZ). Results demonstrated that blocking of TLR2 significantly suppressed the enhanced left ventricular end-diastolic dimension (LVEDD), left ventricular end syst...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Lei L, Hu H, Lei Y, Feng J Tags: Biochem Biophys Res Commun Source Type: research

Involvement of follistatin-like 3 in preeclampsia.
DISCUSSION: Aberrant expression of FSTL3 in preeclampsia led to the dysfunction of trophoblast, indicating its involvement in the pathogenesis of preeclampsia. PMID: 30454705 [PubMed - in process] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Xie J, Xu Y, Wan L, Wang P, Wang M, Dong M Tags: Biochem Biophys Res Commun Source Type: research

Combinatory inhibition of TRIM65 and MDM2 in lung cancer cells.
Abstract In addition to the involvement in white matter lesion, tripartite-motif protein family member 65 (TRIM65) has also been implicated in tumorigenesis as a potential oncogene. However, the underlining mechanisms of TRIM65 functions and its clinical implication still remain to be further elucidated. In the present study, we found that TRIM65 binds to the N-terminus of p53 tumor suppressor and thus competes with MDM2 for p53 binding. Intriguingly, analysis of the Cancer Genome Atlas (TCGA) gene alteration database revealed that elevated expression of TRIM65 is mutually exclusive to MDM2 up-regulation in human ...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Chen G, Zhou T, Liu Y, Yu Z Tags: Biochem Biophys Res Commun Source Type: research

Pazopanib-induced changes in protein expression signatures of extracellular vesicles in synovial sarcoma.
The objective of this study was to elucidate the role of proteins secreted from synovial sarcoma cells in the regulatory network underlying pazopanib response. We performed a comprehensive analysis of expression of proteins secreted from four synovial sarcoma cell lines (SYO-1, HS-SYII, 1273/99, and YaFuSS) using mass spectroscopy. Comparison of up-regulated proteins in cells, extracellular vesicles-free conditioned media, and extracellular vesicles revealed significantly up-regulated Wnt in synovial sarcoma vesicles. Furthermore, we compared protein signatures of cells, conditioned media, and extracellular vesicles before...
Source: Biochemical and Biophysical Research communications - November 22, 2018 Category: Biochemistry Authors: Shiozawa K, Yoshioka Y, Qiao Z, Shuting J, Ochiya T, Kondo T Tags: Biochem Biophys Res Commun Source Type: research

STAM-binding protein regulates melanoma metastasis through SLUG stabilization.
In this study, we demonstrate that STAMBP is a key molecule for regulating melanoma migration and invasion, but not survival, by knocking down STAMBP in vitro. STAMBP regulates SLUG expression through a post-transcriptional mechanism to control protein stability and further contributes to the in vivo metastatic potential of melanoma. Collectively, these results indicate the importance of STAMBP in melanoma metastasis by regulating SLUG. It is therefore a potential therapeutic target. PMID: 30454887 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Iwakami Y, Yokoyama S, Watanabe K, Hayakawa Y Tags: Biochem Biophys Res Commun Source Type: research

Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1 / DDAH / PPAR- γ/ eNOS pathways in streptozotocin-induced diabetic rats.
This study investigated the efficacy and mechanism of probucol in improving erectile function in streptozotocin-induced diabetic rats. METHODS: In our study, thirty 12-week-old Sprague-Dawley male rats were fasted for 12 h. All rats received a 1-time injection of intraperitoneal streptozotocin(60 mg/kg) or vehicle. After 72 h, STZ-treated rats (with random blood glucose concentrations consistently greater than 16.7 mmol/L) were considered diabetic. The diabetic rats were randomly assigned into 2 groups and treated with daily gavage feedings of probucol at doses of 0 and 500 mg/kg for 12 weeks. A positive con...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Hu LL, Zhang KQ, Tian T, Zhang H, Fu Q Tags: Biochem Biophys Res Commun Source Type: research

Linc01230, transcriptionally regulated by PPAR γ, is identified as a novel modifier in endothelial function.
In this study, we not only verified that PPARγ could antagonize the adverse effects brought from ox-LDL, but also found a novel factor related to PPARγ, named linc01230. According to our study, PPARγ transcriptionally regulated linc01230 by specifically combining with two binding regions, which have superposition effect, in the upstream of linc01230 promoter. In addition, linc01230 reduced ox-LDL induced endothelial dysfunction and affected the phosphorylation of Akt. These results conclude linc01230 as a novel modifier in PPARγ-mediated activation of Akt in endothelial function. PMID: 30454889...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Liu L, Wang Z, Jia J, Shi Y, Lian T, Han X Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA GClnc1 promotes tumorigenesis in osteosarcoma by inhibiting p53 signaling.
This study shed light on the oncogene role of lncRNA GClnc1, a new modulator of p53 signaling, in OS. PMID: 30454890 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Sui Y, Han Y, Zhao X, Li D, Li G Tags: Biochem Biophys Res Commun Source Type: research

Suppressing NLRP2 expression accelerates hepatic steatosis: A  mechanism involving inflammation and oxidative stress.
Suppressing NLRP2 expression accelerates hepatic steatosis: A mechanism involving inflammation and oxidative stress. Biochem Biophys Res Commun. 2018 Nov 16;: Authors: Li C, Liu Q, Xie L Abstract Nonalcoholic fatty liver disease (NAFLD) is characterized by lipid accumulation and inflammation in the liver, contributing to a broad spectrum of severe pathologies, such as metabolic syndrome and hepatocellular carcinoma. Presently, the pathogenesis that attributes to NAFLD has not been fully understood. NLRP2 has been shown to inhibit the NF-κB signaling, and thus may contribute to regulate the ...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Li C, Liu Q, Xie L Tags: Biochem Biophys Res Commun Source Type: research

The positive feedback between lncRNA TNK2-AS1 and STAT3 enhances angiogenesis in non-small cell lung cancer.
Abstract Evidence has shown the importance of long non-coding RNAs (lncRNAs) during angiogenesis and lung cancer progression. However, the potential functions of TNK2-AS1 in non-small cell lung cancer (NSCLC) remain elusive. By lncRNA profiling, we identified TNK2-AS1 as a novel oncogenic lncRNA in NSCLC. TNK2-AS1 was significantly upregulated in NSCLC and correlated with poor prognosis. We found that TNK2-AS1 could increase viability and migration of NSCLC cells in vitro. TNK2-AS1 also promoted NSCLC xenograft tumor growth and metastasis in vivo. TNK2-AS1 could interact with STAT3 to increase its protei...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Wang Y, Han D, Pan L, Sun J Tags: Biochem Biophys Res Commun Source Type: research

Interleukin-7 stimulation inhibits nephrin activation and induces podocyte injury.
In this study, we found that the interleukin-7 receptor (IL-7R) was induced in the glomeruli of adriamycin (ADR)-induced mouse nephropathy, a rodent model of nephrotic syndrome. In addition, IL-7R was also induced by ADR in mouse podocytes cultured in vitro. Functionally, we discovered that IL-7R activation through the stimulation of recombinant IL-7 induced apoptosis of podocytes, and moreover, IL-7 stimulation inhibited nephrin activation and caused actin cytoskeleton disorganization, indicating that IL-7 stimulation induces podocyte injury. Furthermore, IL-7 stimulation impaired the filtration barrier function of p...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Zhai S, Zhao L, Zhang Y, Ma Q Tags: Biochem Biophys Res Commun Source Type: research

Effects of none-steroidal anti-inflammatory and antibiotic drugs on the oral immune system and oral microbial composition in rats.
CONCLUSION: Our results indicate that antibiotic and none-steroidal anti-inflammatory drugs could influence the oral microbiota composition, which could also destroy the original oral micro-ecosystem environment. The non-antibiotic drug effect on the oral microbiota and oral immune system similar to the antibiotic drug. All these changes may have a negative influence on host health. PMID: 30454894 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Cheng X, Huang F, Zhang K, Yuan X, Song C Tags: Biochem Biophys Res Commun Source Type: research

Chemoattractant receptors activate, recruit and capture G proteins for wide range chemotaxis.
Abstract The wide range sensing of extracellular signals is a common feature of various sensory cells. Eukaryotic chemotactic cells driven by GPCRs and their cognate G proteins are one example. This system endows the cells directional motility towards their destination over long distances. There are several mechanisms to achieve the long dynamic range, including negative regulation of the receptors upon ligand interaction and spatial regulation of G proteins, as we found recently. However, these mechanisms are insufficient to explain the 105-fold range of chemotaxis seen in Dictyostelium. Here, we reveal that the ...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Miyanaga Y, Kamimura Y, Kuwayama H, Devreotes PN, Ueda M Tags: Biochem Biophys Res Commun Source Type: research

Targeting TPX2 suppresses proliferation and promotes apoptosis via repression of the PI3k/AKT/P21 signaling pathway and activation of p53 pathway in breast cancer.
In this study, the differentially expressed genes including TPX2 were screened in GEO database for gene expression microarray of breast cancer. The TPX2 expression level was significantly increased in breast cancer cells and the breast malignant tissues compared with those controls. In vitro experiment further confirmed that knockdown of TPX2 by small hairpin RNA inhibited breast cancer cell proliferatio, migration, and induced cell apoptosis. TPX2 silencing decreased the expression of PI3K and extent of AKT phosphorylation, as well as increased expression of p53 and p21. Taken together, our findings indicate that TPX...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Chen M, Zhang H, Zhang G, Zhong A, Ma Q, Kai J, Tong Y, Xie S, Wang Y, Zheng H, Guo L, Lu R Tags: Biochem Biophys Res Commun Source Type: research

High glucose-induced circHIPK3 downregulation mediates endothelial cell injury.
Abstract High glucose (HG) induces vascular endothelial cell injury. However, the underlying mechanisms are poorly understood. Circular RNA HIPK3 (circHIPK3) is a highly conserved non-coding RNA. Here we show that circHIPK3 is downregulated in HG-treated human umbilical vein endothelial cells (HUVECs) and in primary aortic endothelial cells (HAECs) from diabetic patients. In both HUVECs and HAECs, lentivirus-mediated circHIPK3 overexpression inhibited HG-induced cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted siRNA exacerbated HG-induced endothelial cell death and apoptosis. Further, circHIPK...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Cao Y, Yuan G, Zhang Y, Lu R Tags: Biochem Biophys Res Commun Source Type: research

Insulin-like growth factor binding Protein-3 suppresses osteoblast differentiation via bone morphogenetic protein-2.
In this study, we investigated the function of IGFBP-3 in osteoblast differentiation. Our results showed that IGFBP-3 decreases the expression of osteoblast differentiation markers, whose expression is enhanced by bone morphogenetic protein-2 (BMP-2). IGFBP-3 also reduced BMP-2 effect on ALP activity and mineral nodule formation. In addition, IGFBP-3 suppresses the activity of the Smad Binding Element (SBE) reporter, induced by BMP-2 signaling. These results suggest that IGFBP-3 inhibits osteoblast differentiation through the BMP-2 signal pathway, and that IGFBP-3 might play a role in bone mass maintenance in an IGF-1-depe...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Eguchi K, Akiba Y, Akiba N, Nagasawa M, Cooper LF, Uoshima K Tags: Biochem Biophys Res Commun Source Type: research

Minocycline prevents peroxidative permeabilization of cardiolipin-containing bilayer lipid membranes mediated by cytochrome c.
Abstract Peroxidase activity of cytochrome c stimulated by interaction of the protein with cardiolipin is considered to be involved in the induction of mitochondrial apoptosis associated with cytochrome c release from mitochondria. In model systems, this activity has been previously shown to cause permeabilization of cardiolipin-containing membranes. Here, we found that the antibiotic minocycline, known to have neuroprotective properties, inhibited both the binding of cyt c to cardiolipin-containing membranes and the cyt c/H2O2-induced liposome permeabilization. The results could be relevant to inhibition of cyt c...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Firsov AM, Kotova EA, Antonenko YN Tags: Biochem Biophys Res Commun Source Type: research

Rnf112 deletion protects brain against intracerebral hemorrhage (ICH) in mice by inhibiting TLR-4/NF- κB pathway.
Rnf112 deletion protects brain against intracerebral hemorrhage (ICH) in mice by inhibiting TLR-4/NF-κB pathway. Biochem Biophys Res Commun. 2018 Nov 16;: Authors: Zhang F, Zhang C Abstract Intracerebral hemorrhage (ICH) is reported as a common and often fatal type of stroke accompanied with high morbidity and mortality, and it frequently results in long-lasting neurological dysfunctions. However, the pathogenesis that contributes to ICH has not been fully understood. Rnf112, also known as Znf179, is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain an...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Zhang F, Zhang C Tags: Biochem Biophys Res Commun Source Type: research

AURKA contributes to the progression of oral squamous cell carcinoma (OSCC) through modulating epithelial-to-mesenchymal transition (EMT) and apoptosis via the regulation of ROS.
Abstract Oral squamous cell carcinoma (OSCC) is known as one of the most common cancer influencing the head and neck region. However, the molecular mechanisms revealing OSCC progression is largely unclear. Aurora kinase A (AURKA) is a serine-threonine kinase that functions in mitotic spindle formation and chromosome segregation, and is associated with the progression of human cancers. But its role in regulating OSCC development has not yet been investigated. In the study, we found that AURKA expression was up-regulated in OSCC cell lines and tumor specimens from patients. OSCC patients with high expression of AURK...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Dawei H, Honggang D, Qian W Tags: Biochem Biophys Res Commun Source Type: research

MFAP5 suppression inhibits migration/invasion, regulates cell cycle and induces apoptosis via promoting ROS production in cervical cancer.
Abstract Cervical cancer is one of the most lethal types of cancer among female. Microfibrillar-associated protein 5 (MFAP5) is an extracellular matrix (ECM) glycoprotein, and is confirmed to be involved in cell signaling during microfibril assembly, elastinogenesis and cell survival. However, the role of MFAP5 in cervical cancer development and progression remains poorly understood. In the study, MFAP5 was over-expressed in human cervical cancers, and in different cervical cancer cell lines. Patients suffering from cervical cancer with low MFAP5 expression exhibited better survival rate. Suppressing MFAP5 in cerv...
Source: Biochemical and Biophysical Research communications - November 16, 2018 Category: Biochemistry Authors: Li Q, Zhang Y, Jiang Q Tags: Biochem Biophys Res Commun Source Type: research

KATNAL1 is a more active and stable isoform of katanin, and is expressed dominantly in neurons.
Abstract Microtubule severing is essential for reorganization of microtubules during neuronal migration and process elongation. Katanin is a microtubule-severing enzyme, of which the major catalytic subunits are katanin A1 (KATNA1) and katanin A-like 1 (KATNAL1). The domain organization of the two subunits are almost the same; however, little is known about their functional difference. Here, we compared the expression pattern, microtubule-severing activity, intracellular degradation and knockdown phenotype in cultured cells of the two subunits. While KATNA1 was expressed ubiquitously among tissues of young adult m...
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Hatakeyama E, Hayashi K Tags: Biochem Biophys Res Commun Source Type: research

Thioredoxin-interacting protein-derived peptide (TN13) inhibits LPS-induced inflammation by inhibiting p38 MAPK signaling.
Abstract Inflammation comprises an innate immune response, and is mainly induced by macrophages to protect the host from pathogens and mechanical injuries. The p38 mitogen-activated protein kinase (MAPK) pathway is a key regulator of inflammatory responses in macrophages. Here, we investigated the anti-inflammatory effects of thioredoxin-interacting protein-derived peptide (TN13) in macrophages in vitro and in vivo. Human immunodeficiency virus (HIV) trans-activator protein (TAT)-conjugated TN13 (TAT-TN13) was found to penetrate RAW 264.7 cells and decrease p38 MAPK activation in a dose-dependent manne...
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Kim DO, Byun JE, Seong HA, Yoon SR, Choi I, Jung H Tags: Biochem Biophys Res Commun Source Type: research

CD44v-dependent upregulation of xCT is involved in the acquisition of cisplatin-resistance in human lung cancer A549  cells.
CD44v-dependent upregulation of xCT is involved in the acquisition of cisplatin-resistance in human lung cancer A549 cells. Biochem Biophys Res Commun. 2018 Nov 15;: Authors: Horibe S, Kawauchi S, Tanahashi T, Sasaki N, Mizuno S, Rikitake Y Abstract Cisplatin (CDDP) is widely used as an anti-cancer platinum agent but its therapeutic efficacy is limited by acquired drug resistance. To develop a new therapeutic strategy that could overcome this resistance, it is important to characterize CDDP-resistant cancer cells. Here we established human lung cancer A549 cell-derived low- and high-grade CDDP-resis...
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Horibe S, Kawauchi S, Tanahashi T, Sasaki N, Mizuno S, Rikitake Y Tags: Biochem Biophys Res Commun Source Type: research

Hydrogen sulfide ameliorated lipopolysaccharide-induced acute lung injury by inhibiting autophagy through PI3K/Akt/mTOR pathway in mice.
CONCLUSION: Autophagy inhibition through PI3K/Akt/mTOR pathway was involved in H2S prevention of LPS-induced ALI in mice. PMID: 30448177 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Xu X, Li H, Gong Y, Zheng H, Zhao D Tags: Biochem Biophys Res Commun Source Type: research

Hop/STIP1 depletion alters nuclear structure via depletion of nuclear structural protein emerin.
Abstract Hop/STIP1 is a co-chaperone of Hsp70 and Hsp90 that regulates a number of cell biology processes via interactions with cellular proteins. Here we report a new relationship between Hop and the nuclear structural protein emerin in maintenance of nuclear morphology. Depletion or overexpression of Hop resulted in the reduction of emerin protein levels via proteasomal and lysosomal pathways. Co-immunoprecipitation assays confirmed that Hop and emerin are in a common complex, which could accommodate Hsp70 but not Hsp90, and that TPR2AB is required for the association. Loss of Hop or emerin led to a deformation ...
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Kituyi SN, Edkins AL Tags: Biochem Biophys Res Commun Source Type: research

MicroRNA-98-5p ameliorates oxygen-glucose deprivation/reoxygenation (OGD/R)-induced neuronal injury by inhibiting Bach1 and promoting Nrf2/ARE signaling.
In this study, we investigated the role and mechanism of miR-98-5p in regulating neuronal injury induced by oxygen-glucose deprivation/reoxygenation (OGD/R), an in vitro model of cerebral ischemia/reperfusion injury. We found that miR-98 expression was significantly altered in neurons in response to OGD/R treatment. Functional experiments showed that overexpression of miR-98-5p inhibited OGD/R-induced apoptosis and reactive oxygen species (ROS) production in neurons, whereas inhibition of miR-98-5p showed the opposite effect. Interestingly, bioinformatics analysis predicted that BTB and CNC homology 1 (Bach1) was a po...
Source: Biochemical and Biophysical Research communications - November 15, 2018 Category: Biochemistry Authors: Sun X, Li X, Ma S, Guo Y, Li Y Tags: Biochem Biophys Res Commun Source Type: research