The RING-type protein BOI negatively regulates the protein level of a CC-NBS-LRR in Arabidopsis
In conclusion, our results reveal a mechanism for the control of the stability of L5 protein and for the suppressed of L5-triggered cell death by a RING-type E3 ligase through the ubiquitin proteasome system.PMID:34560579 | DOI:10.1016/j.bbrc.2021.09.038 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 24, 2021 Category: Biochemistry Authors: Jianzhong Huang Xiaoqiu Wu Zhiyong Gao Source Type: research

Aromatic and aliphatic residues of the disordered region of TDP-43 are on a fast track for self-assembly
Biochem Biophys Res Commun. 2021 Sep 20;578:110-114. doi: 10.1016/j.bbrc.2021.09.040. Online ahead of print.ABSTRACTThe C-terminal, intrinsically disordered, prion-like domain (PrLD) of TDP-43 promotes liquid condensate and solid amyloid formation. These phase changes are crucial to the normal biological functions of the protein but also for its abnormal aggregation, which is implicated in amyotrophic lateral sclerosis (ALS) and certain dementias. We and other previously found that certain amyloid forms emerge from an intermediate condensed state that acts as a nucleus for fibrillization. To quantitatively ascertain the ro...
Source: Biochemical and Biophysical Research communications - September 24, 2021 Category: Biochemistry Authors: Douglas V Laurents Cristiana Stuani David Pantoja-Uceda Emanuele Buratti Miguel Mompe án Source Type: research

Effectiveness of dual-detergent strategy using Triton X-100 in membrane protein purification
Biochem Biophys Res Commun. 2021 Sep 20;578:122-128. doi: 10.1016/j.bbrc.2021.09.031. Online ahead of print.ABSTRACTMembrane solubilization by detergents is a critical step for successful membrane protein purification. Alkyl maltoside detergents such as DDM and DM are very expensive and are commonly used to produce most of the high-quality proteins in stable and functional form. Recently, dual-detergent strategy using inexpensive detergents for membrane solubilization step has been shown to be highly effective in purifying different classes of membrane proteins in a cost-effective manner. In this work, we have monitored th...
Source: Biochemical and Biophysical Research communications - September 24, 2021 Category: Biochemistry Authors: Anindita Das Arpan Bysack H Raghuraman Source Type: research

Proteasomal activator 28 gamma stabilizes hepatitis B virus X protein by competitively inhibiting the Siah-1-mediated proteasomal degradation
Biochem Biophys Res Commun. 2021 Sep 16;578:97-103. doi: 10.1016/j.bbrc.2021.09.028. Online ahead of print.ABSTRACTProteasomal activator 28 gamma (PA28γ) upregulates the levels of HBx, a regulatory protein of hepatitis B virus (HBV) to stimulate HBV replication; however, the detailed mechanism remains unknown. Here, we found that PA28γ impaired the ability of seven in absentia homolog 1 (Siah-1) as an E3 ubiquitin ligase of HBx. PA28γ competitively inhibited the binding of Siah-1 to HBx in human hepatoma cells. Accordingly, PA28γ increased the stability of HBx and decreased HBx ubiquitination, aboli...
Source: Biochemical and Biophysical Research communications - September 23, 2021 Category: Biochemistry Authors: Jiwoo Han Haeji Kim Hyerin Jeong Hyunyoung Yoon Kyung Lib Jang Source Type: research

PPBP as a marker of diabetic nephropathy podocyte injury via Bioinformatics Analysis
Biochem Biophys Res Commun. 2021 Aug 30;577:165-172. doi: 10.1016/j.bbrc.2021.08.087. Online ahead of print.ABSTRACTDiabetic nephropathy (DN) is a type of kidney injuries associated with diabetes mellitus and the prevalence of DN has increased dramatically. However, DN still pose problems in therapy, and prognosis. Identifying new DN biomarkers would be helpful in reducing morbidity and mortality from DN and developing novel preventive approaches. In the study, from GSE36336 dataset with DN glomeruli samples, we screened for 238 differentially expressed genes. Enrichment analysis were performed to find out biological funct...
Source: Biochemical and Biophysical Research communications - September 23, 2021 Category: Biochemistry Authors: Fengxia Zhang Nan Jiang Yan Gao Zuyan Fan Quhuan Li Guibao Ke Bohou Li Qiong Wu Ruiquan Xu Shuangxin Liu Source Type: research

Cox15 is a novel oncogene that required for lung cancer cell proliferation
Biochem Biophys Res Commun. 2021 Sep 14;578:70-76. doi: 10.1016/j.bbrc.2021.09.010. Online ahead of print.ABSTRACTLung cancer is one of the most malignant and prevalent tumors and accounts for the vast majority of cancer death worldwide. However, the molecular mechanisms underlying lung cancer progression are poorly understood. Here, we reveal that both transcription and protein expression levels of Cox15 were increased in lung cancer. Nrf2 specifically binds to the Cox15 promoter and triggers Cox15 expression at the transcriptional level. Cox15 functions as a novel oncogene that facilitates lung cancer cell proliferation....
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Cong Zhang Ning Li Ying-Ying Liu Tian Yuan Song Yang Xiang-Peng Wang Source Type: research

Upregulation of α enolase (ENO1) crotonylation in colorectal cancer and its promoting effect on cancer cell metastasis
Biochem Biophys Res Commun. 2021 Sep 16;578:77-83. doi: 10.1016/j.bbrc.2021.09.027. Online ahead of print.ABSTRACTLysine crotonylation (Kcr) is a newly identified protein translational modification and is involved in major biological processes including glycolysis, but its role in colorectal cancer (CRC) is unknown. Here, we found that the Kcr of α enolase (ENO1) was significantly elevated in human CRC tissues compared with the paratumoral tissues. CREB-binding protein (CBP) functioned as a crotonyltranferase of ENO1, and SIRT2 was involved in the decrotonylation of ENO1. Using quantitative mass spectrometry for crot...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Jia-Yi Hou Jing Cao Li-Juan Gao Fu-Peng Zhang Jing Shen Lan Zhou Jian-Yun Shi Yan-Lin Feng Zi Yan De-Ping Wang Ji-Min Cao Source Type: research

Cryo-EM structure of the dual incretin receptor agonist, peptide-19, in complex with the glucagon-like peptide-1 receptor
In this study, we solved the structure of peptide-19 bound to the GLP-1R in complex with Gs protein, and compared the structure and dynamics of this complex to that of published structures of GLP-1R:Gs in complex with other receptor agonists. Unlike other peptide-bound receptor complexes, peptide-19:GLP-1R:Gs demonstrated a more open binding pocket where transmembrane domain (TM) 6, TM7 and the interconnecting extracellular loop 3 (ECL3) were located away from the peptide, with no interactions between peptide-19 and TM6/ECL3. Analysis of conformational variance of the complex revealed that peptide-19 was highly dynamic and...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Rachel M Johnson Xin Zhang Sarah J Piper Theodore J Nettleton Teresa H Vandekolk Christopher J Langmead Radostin Danev Patrick M Sexton Denise Wootten Source Type: research

The in  vitro and in vivo efficacy of CT-P59 against Gamma, Delta and its associated variants of SARS-CoV-2
Biochem Biophys Res Commun. 2021 Sep 13;578:91-96. doi: 10.1016/j.bbrc.2021.09.023. Online ahead of print.ABSTRACTThe SARS-CoV-2 variant is rapidly spreading across the world and causes to resurge infections. We previously reported that CT-P59 presented its in vivo potency against Beta variants, despite its reduced activity in cell experiments. Yet, it remains uncertain to exert the antiviral effect of CT-P59 on Gamma, Delta and its associated variants (L452R). To tackle this question, we carried out cell tests and animal studies. CT-P59 showed neutralization against Gamma, Delta, Epsilon, and Kappa variants in cells, with...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Dong-Kyun Ryu Bobin Kang Hanmi Noh Sun-Je Woo Min-Ho Lee Patricia M Nuijten Jong-In Kim Ji-Min Seo Cheolmin Kim Minsoo Kim Eunji Yang Gippeum Lim Seong-Gyu Kim Su-Kyeong Eo Jung-Ah Choi Manki Song Sang-Seok Oh Hyo-Young Chung Aloys Sl Tijsma Carel A van B Source Type: research

Cox15 is a novel oncogene that required for lung cancer cell proliferation
Biochem Biophys Res Commun. 2021 Sep 14;578:70-76. doi: 10.1016/j.bbrc.2021.09.010. Online ahead of print.ABSTRACTLung cancer is one of the most malignant and prevalent tumors and accounts for the vast majority of cancer death worldwide. However, the molecular mechanisms underlying lung cancer progression are poorly understood. Here, we reveal that both transcription and protein expression levels of Cox15 were increased in lung cancer. Nrf2 specifically binds to the Cox15 promoter and triggers Cox15 expression at the transcriptional level. Cox15 functions as a novel oncogene that facilitates lung cancer cell proliferation....
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Cong Zhang Ning Li Ying-Ying Liu Tian Yuan Song Yang Xiang-Peng Wang Source Type: research

Upregulation of α enolase (ENO1) crotonylation in colorectal cancer and its promoting effect on cancer cell metastasis
Biochem Biophys Res Commun. 2021 Sep 16;578:77-83. doi: 10.1016/j.bbrc.2021.09.027. Online ahead of print.ABSTRACTLysine crotonylation (Kcr) is a newly identified protein translational modification and is involved in major biological processes including glycolysis, but its role in colorectal cancer (CRC) is unknown. Here, we found that the Kcr of α enolase (ENO1) was significantly elevated in human CRC tissues compared with the paratumoral tissues. CREB-binding protein (CBP) functioned as a crotonyltranferase of ENO1, and SIRT2 was involved in the decrotonylation of ENO1. Using quantitative mass spectrometry for crot...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Jia-Yi Hou Jing Cao Li-Juan Gao Fu-Peng Zhang Jing Shen Lan Zhou Jian-Yun Shi Yan-Lin Feng Zi Yan De-Ping Wang Ji-Min Cao Source Type: research

Cryo-EM structure of the dual incretin receptor agonist, peptide-19, in complex with the glucagon-like peptide-1 receptor
In this study, we solved the structure of peptide-19 bound to the GLP-1R in complex with Gs protein, and compared the structure and dynamics of this complex to that of published structures of GLP-1R:Gs in complex with other receptor agonists. Unlike other peptide-bound receptor complexes, peptide-19:GLP-1R:Gs demonstrated a more open binding pocket where transmembrane domain (TM) 6, TM7 and the interconnecting extracellular loop 3 (ECL3) were located away from the peptide, with no interactions between peptide-19 and TM6/ECL3. Analysis of conformational variance of the complex revealed that peptide-19 was highly dynamic and...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Rachel M Johnson Xin Zhang Sarah J Piper Theodore J Nettleton Teresa H Vandekolk Christopher J Langmead Radostin Danev Patrick M Sexton Denise Wootten Source Type: research

The in  vitro and in vivo efficacy of CT-P59 against Gamma, Delta and its associated variants of SARS-CoV-2
Biochem Biophys Res Commun. 2021 Sep 13;578:91-96. doi: 10.1016/j.bbrc.2021.09.023. Online ahead of print.ABSTRACTThe SARS-CoV-2 variant is rapidly spreading across the world and causes to resurge infections. We previously reported that CT-P59 presented its in vivo potency against Beta variants, despite its reduced activity in cell experiments. Yet, it remains uncertain to exert the antiviral effect of CT-P59 on Gamma, Delta and its associated variants (L452R). To tackle this question, we carried out cell tests and animal studies. CT-P59 showed neutralization against Gamma, Delta, Epsilon, and Kappa variants in cells, with...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Dong-Kyun Ryu Bobin Kang Hanmi Noh Sun-Je Woo Min-Ho Lee Patricia M Nuijten Jong-In Kim Ji-Min Seo Cheolmin Kim Minsoo Kim Eunji Yang Gippeum Lim Seong-Gyu Kim Su-Kyeong Eo Jung-Ah Choi Manki Song Sang-Seok Oh Hyo-Young Chung Aloys Sl Tijsma Carel A van B Source Type: research

Cox15 is a novel oncogene that required for lung cancer cell proliferation
Biochem Biophys Res Commun. 2021 Sep 14;578:70-76. doi: 10.1016/j.bbrc.2021.09.010. Online ahead of print.ABSTRACTLung cancer is one of the most malignant and prevalent tumors and accounts for the vast majority of cancer death worldwide. However, the molecular mechanisms underlying lung cancer progression are poorly understood. Here, we reveal that both transcription and protein expression levels of Cox15 were increased in lung cancer. Nrf2 specifically binds to the Cox15 promoter and triggers Cox15 expression at the transcriptional level. Cox15 functions as a novel oncogene that facilitates lung cancer cell proliferation....
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Cong Zhang Ning Li Ying-Ying Liu Tian Yuan Song Yang Xiang-Peng Wang Source Type: research

Upregulation of α enolase (ENO1) crotonylation in colorectal cancer and its promoting effect on cancer cell metastasis
Biochem Biophys Res Commun. 2021 Sep 16;578:77-83. doi: 10.1016/j.bbrc.2021.09.027. Online ahead of print.ABSTRACTLysine crotonylation (Kcr) is a newly identified protein translational modification and is involved in major biological processes including glycolysis, but its role in colorectal cancer (CRC) is unknown. Here, we found that the Kcr of α enolase (ENO1) was significantly elevated in human CRC tissues compared with the paratumoral tissues. CREB-binding protein (CBP) functioned as a crotonyltranferase of ENO1, and SIRT2 was involved in the decrotonylation of ENO1. Using quantitative mass spectrometry for crot...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Jia-Yi Hou Jing Cao Li-Juan Gao Fu-Peng Zhang Jing Shen Lan Zhou Jian-Yun Shi Yan-Lin Feng Zi Yan De-Ping Wang Ji-Min Cao Source Type: research

Cryo-EM structure of the dual incretin receptor agonist, peptide-19, in complex with the glucagon-like peptide-1 receptor
In this study, we solved the structure of peptide-19 bound to the GLP-1R in complex with Gs protein, and compared the structure and dynamics of this complex to that of published structures of GLP-1R:Gs in complex with other receptor agonists. Unlike other peptide-bound receptor complexes, peptide-19:GLP-1R:Gs demonstrated a more open binding pocket where transmembrane domain (TM) 6, TM7 and the interconnecting extracellular loop 3 (ECL3) were located away from the peptide, with no interactions between peptide-19 and TM6/ECL3. Analysis of conformational variance of the complex revealed that peptide-19 was highly dynamic and...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Rachel M Johnson Xin Zhang Sarah J Piper Theodore J Nettleton Teresa H Vandekolk Christopher J Langmead Radostin Danev Patrick M Sexton Denise Wootten Source Type: research

The in  vitro and in vivo efficacy of CT-P59 against Gamma, Delta and its associated variants of SARS-CoV-2
Biochem Biophys Res Commun. 2021 Sep 13;578:91-96. doi: 10.1016/j.bbrc.2021.09.023. Online ahead of print.ABSTRACTThe SARS-CoV-2 variant is rapidly spreading across the world and causes to resurge infections. We previously reported that CT-P59 presented its in vivo potency against Beta variants, despite its reduced activity in cell experiments. Yet, it remains uncertain to exert the antiviral effect of CT-P59 on Gamma, Delta and its associated variants (L452R). To tackle this question, we carried out cell tests and animal studies. CT-P59 showed neutralization against Gamma, Delta, Epsilon, and Kappa variants in cells, with...
Source: Biochemical and Biophysical Research communications - September 21, 2021 Category: Biochemistry Authors: Dong-Kyun Ryu Bobin Kang Hanmi Noh Sun-Je Woo Min-Ho Lee Patricia M Nuijten Jong-In Kim Ji-Min Seo Cheolmin Kim Minsoo Kim Eunji Yang Gippeum Lim Seong-Gyu Kim Su-Kyeong Eo Jung-Ah Choi Manki Song Sang-Seok Oh Hyo-Young Chung Aloys Sl Tijsma Carel A van B Source Type: research

PET neuroimaging reveals upregulation of dopamine D2 receptor contributes to amygdaloid dysfunction in rat acute restraint stress model
Biochem Biophys Res Commun. 2021 Sep 15:S0006-291X(21)01251-1. doi: 10.1016/j.bbrc.2021.08.068. Online ahead of print.NO ABSTRACTPMID:34538482 | DOI:10.1016/j.bbrc.2021.08.068 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 20, 2021 Category: Biochemistry Authors: Zhi Yan Yue Li Xiaojing Zhang Linfeng Li Yuping Gao Qiaozhen Chen Mei Tian Bin Cong Source Type: research

PET neuroimaging reveals upregulation of dopamine D2 receptor contributes to amygdaloid dysfunction in rat acute restraint stress model
Biochem Biophys Res Commun. 2021 Sep 15:S0006-291X(21)01251-1. doi: 10.1016/j.bbrc.2021.08.068. Online ahead of print.NO ABSTRACTPMID:34538482 | DOI:10.1016/j.bbrc.2021.08.068 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 20, 2021 Category: Biochemistry Authors: Zhi Yan Yue Li Xiaojing Zhang Linfeng Li Yuping Gao Qiaozhen Chen Mei Tian Bin Cong Source Type: research

A splicing variant of Charlatan, a Drosophila REST-like molecule, preferentially localizes to axons
Biochem Biophys Res Commun. 2021 Sep 4;578:35-41. doi: 10.1016/j.bbrc.2021.09.002. Online ahead of print.ABSTRACTNeuron-restrictive silencing factor (NRSF), also known as RE-1 silencing transcription factor (REST), has pivotal functions in many neuron-specific genes. Previous studies revealed that neuron-specific alternative splicing (AS) of REST produces divergent forms of REST variants and provides regulatory complexity in the nervous system. However, the biological significance of these variants in the regulation of neuronal activities remains to be clarified. Here, we revealed that Charlatan (Chn), a Drosophila REST-li...
Source: Biochemical and Biophysical Research communications - September 18, 2021 Category: Biochemistry Authors: Yasutoyo Yamasaki Young-Mi Lim Ryunosuke Minami Leo Tsuda Source Type: research

Designing of various biosensor devices for determination of apoptosis: A comprehensive review
Biochem Biophys Res Commun. 2021 Sep 9;578:42-62. doi: 10.1016/j.bbrc.2021.08.089. Online ahead of print.ABSTRACTApoptosis is a type of cell death caused by the occurrence of both pathological and physiological conditions triggered by ligation of death receptors outside the cell or triggered by DNA damage and/or cytoskeleton disruption. Timely monitoring of apoptosis can effectively help early diagnosis of related diseases and continuous assessment of the effectiveness of drugs. Detecting caspases, a protease family closely related to cellular apoptosis, and its identification as markers of apoptosis is a popular procedure...
Source: Biochemical and Biophysical Research communications - September 18, 2021 Category: Biochemistry Authors: Rumeysa Ak çapınar Bora Garipcan Vahabodin Goodarzi Lokman Uzun Source Type: research

The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana
Biochem Biophys Res Commun. 2021 Sep 14;578:63-69. doi: 10.1016/j.bbrc.2021.09.022. Online ahead of print.ABSTRACTPentatricopeptide repeat (PPR) proteins are a large family in land plants that play a role in organellular RNA processing, editing, and splicing. Here, we identify an Arabidopsis thaliana mutant, gend1-1, which exhibits a short root phenotype with reduced meristem size and cell numbers. Positional cloning of GEND1 revealed that it encodes a PPR protein, and functional analysis showed that GEND1 can bind and edit mitochondrial ccmFn-1 mRNA, causing gend1 mutants to have decreased levels of cytochrome C. GEND1 wa...
Source: Biochemical and Biophysical Research communications - September 18, 2021 Category: Biochemistry Authors: Zhengfei Guo Xiaoyu Wang Zhubing Hu Chengyun Wu Zhenguo Shen Source Type: research

Insights on the disruption of the complex between human positive coactivator 4 and p53 by small molecules
Biochem Biophys Res Commun. 2021 Sep 10;578:15-20. doi: 10.1016/j.bbrc.2021.09.020. Online ahead of print.ABSTRACTInteraction between human positive coactivator 4 (PC4), an abundant nuclear protein, and the tumor suppressor protein p53 plays a crucial role in initiating apoptosis. In certain neurodegenerative diseases PC4 assisted-p53-dependent apoptosis may play a central role. Thus, disruption of p53-PC4 interaction may be a good drug target for certain disease pathologies. A p53-derived short peptide (AcPep) that binds the C-terminal domain of PC4 (C-PC4) is known to disrupt PC4-p53 interaction. To fully characterize it...
Source: Biochemical and Biophysical Research communications - September 17, 2021 Category: Biochemistry Authors: Bhawna Pandey Aditya Dev Debamitra Chakravorty Vishwambhar Vishnu Bhandare Smarajit Polley Siddhartha Roy Gautam Basu Source Type: research

Identification of SRSF3 target mRNAs using inducible TRIBE
In this study, we used inducible TRIBE (targets of RNA binding sites by editing) to identify RNA targets and binding motifs of SRSF3 in human embryonic stem cells (hESCs). We identified 3888 confident binding sites of SRSF3, corresponding to 1222 gene targets. Our results showed that nearly half of the binding sites were distributed in exons, reflecting the alternative splicing function of SRSF3. Motif analysis demonstrated that two of the SRSF3 recognition sequences were the same as the motifs identified in mouse embryonic stem cells, suggesting the recognition sequences of SRSF3 may be conserved in mammals. Overall, our ...
Source: Biochemical and Biophysical Research communications - September 17, 2021 Category: Biochemistry Authors: Siqian Jin Ziwei Xue Jinchun Zhang Ziqi Wang Jin Zhang Di Chen Wanlu Liu Junxin Lin Source Type: research

The largest isoform of Ankyrin-G is required for lattice structure of the axon initial segment
Biochem Biophys Res Commun. 2021 Sep 8;578:28-34. doi: 10.1016/j.bbrc.2021.09.017. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is the most frequent neurodegenerative disease and a common dementia in elderly individuals. Previous studies found a strong correlation between axon initial segment (AIS) defects and AD, but it remains unclear whether AD itself changes the arrangement of AIS components, and the mechanisms by which adaptor proteins and ion channels in the AIS are disturbed in AD are not well understood. With super-resolution structured illumination microscopy (SIM) revealing axonal structures, here we im...
Source: Biochemical and Biophysical Research communications - September 17, 2021 Category: Biochemistry Authors: Yiming Wang Meiling Guan Han Wang Yu Li Karl Zhanghao Peng Xi Yan Zhang Source Type: research

SIRT6 silencing overcomes resistance to sorafenib by promoting ferroptosis in gastric cancer
This study presents that SIRT6 silencing overcomes sorafenib resistance by promoting ferroptosis, which is a novel form of cell death. Mechanistically, SIRT6 inhibition led to the inactivation of the Keap1/Nrf2 signalling pathway and downregulation of GPX4. The overexpression of GPX4 or activation of Keap1/Nrf2 reverses the effects of the downregulation of SIRT6 on sorafenib-induced ferroptosis. Thus, targeting the SIRT6/Keap1/Nrf2/GPX4 signalling pathway may be a potential strategy for overcoming sorafenib resistance in GC.PMID:34530350 | DOI:10.1016/j.bbrc.2021.08.080 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 16, 2021 Category: Biochemistry Authors: Shunv Cai Shuang Fu Weikang Zhang Xiaohong Yuan Yun Cheng Jun Fang Source Type: research

Developmentally regulated GTP-binding protein-2 regulates adipocyte differentiation
This study revealed increases in body weight and adiposity in DRG2 transgenic (Tg) mice overexpressing DRG2. Consistent with these results, DRG2 Tg mice showed increased expression of genes involved in adipogenesis and lipid metabolism in the white adipose tissue. DRG2 was also identified to control adipogenesis by cooperating with peroxisome proliferator activated receptor-γ (PPAR-γ) in cultured adipocytes. Overall, the findings of the current study suggest that DRG2 plays an active role in regulating adipocyte differentiation, and thus participates in the development of obesity during exposure to a fat-rich d...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Byong Seo Park Hye Li Im Nal Ae Yoon Thai Hien Tu Jeong Woo Park Jae Geun Kim Byung Ju Lee Source Type: research

UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1
Biochem Biophys Res Commun. 2021 Sep 6;578:7-14. doi: 10.1016/j.bbrc.2021.08.057. Online ahead of print.ABSTRACTUbiquitin-conjugating enzyme E2S (UBE2S), an important E2 enzyme in the process of ubiquitination, has exhibited oncogenic activities in various malignant tumors. However, it remains unknown whether UBE2S plays a role in urinary bladder cancer (UBC) development. In the current study, our data confirmed UBE2S upregulation in UBC. In vitro and in vivo experiments demonstrated that UBE2S knockdown resulted in attenuated proliferation and enhanced apoptosis, which was inverse to the phenotypes with UBE2S overexpressi...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Hao Tang Tong Fang Meng Ji Jun-Ping Wang Le-Le Song Qiu-Yan Zhang Jin-Sheng Wu Source Type: research

Developmentally regulated GTP-binding protein-2 regulates adipocyte differentiation
This study revealed increases in body weight and adiposity in DRG2 transgenic (Tg) mice overexpressing DRG2. Consistent with these results, DRG2 Tg mice showed increased expression of genes involved in adipogenesis and lipid metabolism in the white adipose tissue. DRG2 was also identified to control adipogenesis by cooperating with peroxisome proliferator activated receptor-γ (PPAR-γ) in cultured adipocytes. Overall, the findings of the current study suggest that DRG2 plays an active role in regulating adipocyte differentiation, and thus participates in the development of obesity during exposure to a fat-rich d...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Byong Seo Park Hye Li Im Nal Ae Yoon Thai Hien Tu Jeong Woo Park Jae Geun Kim Byung Ju Lee Source Type: research

UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1
Biochem Biophys Res Commun. 2021 Sep 6;578:7-14. doi: 10.1016/j.bbrc.2021.08.057. Online ahead of print.ABSTRACTUbiquitin-conjugating enzyme E2S (UBE2S), an important E2 enzyme in the process of ubiquitination, has exhibited oncogenic activities in various malignant tumors. However, it remains unknown whether UBE2S plays a role in urinary bladder cancer (UBC) development. In the current study, our data confirmed UBE2S upregulation in UBC. In vitro and in vivo experiments demonstrated that UBE2S knockdown resulted in attenuated proliferation and enhanced apoptosis, which was inverse to the phenotypes with UBE2S overexpressi...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Hao Tang Tong Fang Meng Ji Jun-Ping Wang Le-Le Song Qiu-Yan Zhang Jin-Sheng Wu Source Type: research

Developmentally regulated GTP-binding protein-2 regulates adipocyte differentiation
This study revealed increases in body weight and adiposity in DRG2 transgenic (Tg) mice overexpressing DRG2. Consistent with these results, DRG2 Tg mice showed increased expression of genes involved in adipogenesis and lipid metabolism in the white adipose tissue. DRG2 was also identified to control adipogenesis by cooperating with peroxisome proliferator activated receptor-γ (PPAR-γ) in cultured adipocytes. Overall, the findings of the current study suggest that DRG2 plays an active role in regulating adipocyte differentiation, and thus participates in the development of obesity during exposure to a fat-rich d...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Byong Seo Park Hye Li Im Nal Ae Yoon Thai Hien Tu Jeong Woo Park Jae Geun Kim Byung Ju Lee Source Type: research

UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1
Biochem Biophys Res Commun. 2021 Sep 6;578:7-14. doi: 10.1016/j.bbrc.2021.08.057. Online ahead of print.ABSTRACTUbiquitin-conjugating enzyme E2S (UBE2S), an important E2 enzyme in the process of ubiquitination, has exhibited oncogenic activities in various malignant tumors. However, it remains unknown whether UBE2S plays a role in urinary bladder cancer (UBC) development. In the current study, our data confirmed UBE2S upregulation in UBC. In vitro and in vivo experiments demonstrated that UBE2S knockdown resulted in attenuated proliferation and enhanced apoptosis, which was inverse to the phenotypes with UBE2S overexpressi...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Hao Tang Tong Fang Meng Ji Jun-Ping Wang Le-Le Song Qiu-Yan Zhang Jin-Sheng Wu Source Type: research

Developmentally regulated GTP-binding protein-2 regulates adipocyte differentiation
This study revealed increases in body weight and adiposity in DRG2 transgenic (Tg) mice overexpressing DRG2. Consistent with these results, DRG2 Tg mice showed increased expression of genes involved in adipogenesis and lipid metabolism in the white adipose tissue. DRG2 was also identified to control adipogenesis by cooperating with peroxisome proliferator activated receptor-γ (PPAR-γ) in cultured adipocytes. Overall, the findings of the current study suggest that DRG2 plays an active role in regulating adipocyte differentiation, and thus participates in the development of obesity during exposure to a fat-rich d...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Byong Seo Park Hye Li Im Nal Ae Yoon Thai Hien Tu Jeong Woo Park Jae Geun Kim Byung Ju Lee Source Type: research

UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1
Biochem Biophys Res Commun. 2021 Sep 6;578:7-14. doi: 10.1016/j.bbrc.2021.08.057. Online ahead of print.ABSTRACTUbiquitin-conjugating enzyme E2S (UBE2S), an important E2 enzyme in the process of ubiquitination, has exhibited oncogenic activities in various malignant tumors. However, it remains unknown whether UBE2S plays a role in urinary bladder cancer (UBC) development. In the current study, our data confirmed UBE2S upregulation in UBC. In vitro and in vivo experiments demonstrated that UBE2S knockdown resulted in attenuated proliferation and enhanced apoptosis, which was inverse to the phenotypes with UBE2S overexpressi...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Hao Tang Tong Fang Meng Ji Jun-Ping Wang Le-Le Song Qiu-Yan Zhang Jin-Sheng Wu Source Type: research

Developmentally regulated GTP-binding protein-2 regulates adipocyte differentiation
This study revealed increases in body weight and adiposity in DRG2 transgenic (Tg) mice overexpressing DRG2. Consistent with these results, DRG2 Tg mice showed increased expression of genes involved in adipogenesis and lipid metabolism in the white adipose tissue. DRG2 was also identified to control adipogenesis by cooperating with peroxisome proliferator activated receptor-γ (PPAR-γ) in cultured adipocytes. Overall, the findings of the current study suggest that DRG2 plays an active role in regulating adipocyte differentiation, and thus participates in the development of obesity during exposure to a fat-rich d...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Byong Seo Park Hye Li Im Nal Ae Yoon Thai Hien Tu Jeong Woo Park Jae Geun Kim Byung Ju Lee Source Type: research

UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1
Biochem Biophys Res Commun. 2021 Sep 6;578:7-14. doi: 10.1016/j.bbrc.2021.08.057. Online ahead of print.ABSTRACTUbiquitin-conjugating enzyme E2S (UBE2S), an important E2 enzyme in the process of ubiquitination, has exhibited oncogenic activities in various malignant tumors. However, it remains unknown whether UBE2S plays a role in urinary bladder cancer (UBC) development. In the current study, our data confirmed UBE2S upregulation in UBC. In vitro and in vivo experiments demonstrated that UBE2S knockdown resulted in attenuated proliferation and enhanced apoptosis, which was inverse to the phenotypes with UBE2S overexpressi...
Source: Biochemical and Biophysical Research communications - September 14, 2021 Category: Biochemistry Authors: Hao Tang Tong Fang Meng Ji Jun-Ping Wang Le-Le Song Qiu-Yan Zhang Jin-Sheng Wu Source Type: research

TUBA1C expression promotes proliferation by regulating the cell cycle and indicates poor prognosis in glioma
This study demonstrated that high TUBA1C expression correlated with poor outcomes in glioma patients and that knocking down TUBA1C suppressed glioma cell proliferation via cell cycle arrest. In addition, TUBA1C might be a therapeutic biomarker for gliomas.PMID:34517210 | DOI:10.1016/j.bbrc.2021.08.079 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 13, 2021 Category: Biochemistry Authors: Shikai Gui Peng Chen Yu Liu Qiaorong Chen Tianxiang Cheng Shulong Lv Tong Zhou Zhen Song Juexian Xiao Wei He Shengtao Yuan Zujue Cheng Source Type: research

Etmopterus lantern sharks use coelenterazine as the substrate for their luciferin-luciferase bioluminescence system
In this study, we detected both coelenterazine and coelenterazine-dependent luciferase activity in the ventral photophore tissue of Etmopterus molleri. The results suggested that bioluminescence in lantern sharks is produced using coelenterazine as the substrate for the luciferin-luciferase reaction, as some luminous bony fishes.PMID:34517211 | DOI:10.1016/j.bbrc.2021.09.007 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 13, 2021 Category: Biochemistry Authors: Gaku Mizuno Daichi Yano Jos é Paitio Hiromitsu Endo Yuichi Oba Source Type: research

Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells
Biochem Biophys Res Commun. 2021 Sep 10;577:146-151. doi: 10.1016/j.bbrc.2021.09.015. Online ahead of print.ABSTRACTThe human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a...
Source: Biochemical and Biophysical Research communications - September 13, 2021 Category: Biochemistry Authors: Michihito Sasaki Mai Kishimoto Yukari Itakura Koshiro Tabata Kittiya Intaruck Kentaro Uemura Shinsuke Toba Takao Sanaki Akihiko Sato William W Hall Yasuko Orba Hirofumi Sawa Source Type: research

Protein-DNA complex structure modeling based on structural template
Biochem Biophys Res Commun. 2021 Sep 8;577:152-157. doi: 10.1016/j.bbrc.2021.09.018. Online ahead of print.ABSTRACTDNA-binding is an important feature of proteins, and protein-DNA interaction involves in many life processes. Various computational methods have been developed to predict protein-DNA complex structures due to the difficulty of experimentally obtaining protein-DNA complex structures. However, prediction of protein-DNA complex is still a challenging problem compared with prediction of protein-RNA complex, this may be due to the large conformational changes between bound and unbound structure in both protein and ...
Source: Biochemical and Biophysical Research communications - September 13, 2021 Category: Biochemistry Authors: Juan Xie Jinfang Zheng Xu Hong Xiaoxue Tong Xudong Liu Qi Song Sen Liu Shiyong Liu Source Type: research

Phospholipase C γ1 represses colorectal cancer growth by inhibiting the Wnt/β-catenin signaling axis
Biochem Biophys Res Commun. 2021 Sep 7;577:103-109. doi: 10.1016/j.bbrc.2021.09.012. Online ahead of print.ABSTRACTAs essential phospholipid signaling regulators, phospholipase C (PLC)s are activated by various extracellular ligands and mediate intracellular signal transduction. PLCγ1 is involved in regulating various cancer cell functions. However, the precise in vivo link between PLCγ1 and cancer behavior remains undefined. To investigate the role of PLCγ1 in colorectal carcinogenesis, we generated an intestinal tissue-specific Plcg1 knock out (KO) in adenomatous polyposis coli (Apc) Min/+ mice. Plcg1 d...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Kyeong Jin Shin Hyun-Jun Jang Yu Jin Lee Yu Geon Lee Pann-Ghill Suh Yong Ryoul Yang Young Chan Chae Source Type: research

Structure and hybridization properties of phosphoryl guanidine oligonucleotides under crowding conditions
Biochem Biophys Res Commun. 2021 Sep 4;577:110-115. doi: 10.1016/j.bbrc.2021.09.001. Online ahead of print.ABSTRACTPhosphoryl guanidine oligonucleotides (PGOs) are promising uncharged analogs of nucleic acids and are used in a variety of applications. The importance of hydration is frequently ignored during the design of modified nucleic acid probes. Such hydrophobic modifications (phosphoryl guanidine) are expected to have a significant impact on the structure and thermal stability of the affected oligo with complementary nucleic acids. Here we aimed to investigate (by the osmotic stress method) hydration changes upon the...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Maria A Kanarskaya Victor M Golyshev Dmitrii V Pyshnyi Alexander A Lomzov Source Type: research

The use of a two-step removal protocol and optimized culture conditions improve development and quality of zona free mouse embryos
Biochem Biophys Res Commun. 2021 Sep 7;577:116-123. doi: 10.1016/j.bbrc.2021.09.013. Online ahead of print.ABSTRACTThe zona pellucida (ZP) plays an important role in both the fertilization and embryonic development. For the successful handling of early stage blastomeres for differentiation analysis, the production of identical twins or quadruplets, nuclear transfer or gene introduction requires the removal of the ZP (ZPR). Although single use of either acidic Tyrode's solution or pronase are commonly used for ZPR, long-term exposure to these agents can result in the inhibition of development with the collapse of the three-...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Weihong Fan Misato Homma Renliang Xu Hiroki Kunii Hanako Bai Manabu Kawahara Toshikazu Kawaguchi Masashi Takahashi Source Type: research

The protein hydration layer in high glucose concentration: Dynamical responses in folded and intrinsically disordered dimeric states
Biochem Biophys Res Commun. 2021 Sep 5;577:124-129. doi: 10.1016/j.bbrc.2021.09.005. Online ahead of print.ABSTRACTThis exposition reveals the effect of glucose as a molecular crowder on the solvent environment in proximity of the protein surface in putative folded (Ubiquitin) and intrinsically disordered (dimeric Amyloid beta) states. Atomistic simulations reveal markedly higher structural perturbation in the disordered systems due to crowding effects, while the folded state retains overall structural fidelity. Key hydrophobic contacts in the disordered dimer are lost. However, glucose induced crowding results in elevated...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Brataraj Ghosh Neelanjana Sengupta Source Type: research

LncRNA AL592284.1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
CONCLUSION: The present study proves that AL592284.1 plays an tumor-promotive role in CC via regulating the miR-30a-5p/Vimentin axis, and inhibition of AL592284.1 may pave the way for CC treatment.PMID:34509725 | DOI:10.1016/j.bbrc.2021.09.014 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Jing Zhang Hong-Li Liu Jing-Bo Liu Yuan Zhang Jing Liu Yan-Hua Li Source Type: research

Phospholipase C γ1 represses colorectal cancer growth by inhibiting the Wnt/β-catenin signaling axis
Biochem Biophys Res Commun. 2021 Sep 7;577:103-109. doi: 10.1016/j.bbrc.2021.09.012. Online ahead of print.ABSTRACTAs essential phospholipid signaling regulators, phospholipase C (PLC)s are activated by various extracellular ligands and mediate intracellular signal transduction. PLCγ1 is involved in regulating various cancer cell functions. However, the precise in vivo link between PLCγ1 and cancer behavior remains undefined. To investigate the role of PLCγ1 in colorectal carcinogenesis, we generated an intestinal tissue-specific Plcg1 knock out (KO) in adenomatous polyposis coli (Apc) Min/+ mice. Plcg1 d...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Kyeong Jin Shin Hyun-Jun Jang Yu Jin Lee Yu Geon Lee Pann-Ghill Suh Yong Ryoul Yang Young Chan Chae Source Type: research

Structure and hybridization properties of phosphoryl guanidine oligonucleotides under crowding conditions
Biochem Biophys Res Commun. 2021 Sep 4;577:110-115. doi: 10.1016/j.bbrc.2021.09.001. Online ahead of print.ABSTRACTPhosphoryl guanidine oligonucleotides (PGOs) are promising uncharged analogs of nucleic acids and are used in a variety of applications. The importance of hydration is frequently ignored during the design of modified nucleic acid probes. Such hydrophobic modifications (phosphoryl guanidine) are expected to have a significant impact on the structure and thermal stability of the affected oligo with complementary nucleic acids. Here we aimed to investigate (by the osmotic stress method) hydration changes upon the...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Maria A Kanarskaya Victor M Golyshev Dmitrii V Pyshnyi Alexander A Lomzov Source Type: research

The use of a two-step removal protocol and optimized culture conditions improve development and quality of zona free mouse embryos
Biochem Biophys Res Commun. 2021 Sep 7;577:116-123. doi: 10.1016/j.bbrc.2021.09.013. Online ahead of print.ABSTRACTThe zona pellucida (ZP) plays an important role in both the fertilization and embryonic development. For the successful handling of early stage blastomeres for differentiation analysis, the production of identical twins or quadruplets, nuclear transfer or gene introduction requires the removal of the ZP (ZPR). Although single use of either acidic Tyrode's solution or pronase are commonly used for ZPR, long-term exposure to these agents can result in the inhibition of development with the collapse of the three-...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Weihong Fan Misato Homma Renliang Xu Hiroki Kunii Hanako Bai Manabu Kawahara Toshikazu Kawaguchi Masashi Takahashi Source Type: research

The protein hydration layer in high glucose concentration: Dynamical responses in folded and intrinsically disordered dimeric states
Biochem Biophys Res Commun. 2021 Sep 5;577:124-129. doi: 10.1016/j.bbrc.2021.09.005. Online ahead of print.ABSTRACTThis exposition reveals the effect of glucose as a molecular crowder on the solvent environment in proximity of the protein surface in putative folded (Ubiquitin) and intrinsically disordered (dimeric Amyloid beta) states. Atomistic simulations reveal markedly higher structural perturbation in the disordered systems due to crowding effects, while the folded state retains overall structural fidelity. Key hydrophobic contacts in the disordered dimer are lost. However, glucose induced crowding results in elevated...
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Brataraj Ghosh Neelanjana Sengupta Source Type: research

LncRNA AL592284.1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
CONCLUSION: The present study proves that AL592284.1 plays an tumor-promotive role in CC via regulating the miR-30a-5p/Vimentin axis, and inhibition of AL592284.1 may pave the way for CC treatment.PMID:34509725 | DOI:10.1016/j.bbrc.2021.09.014 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - September 12, 2021 Category: Biochemistry Authors: Jing Zhang Hong-Li Liu Jing-Bo Liu Yuan Zhang Jing Liu Yan-Hua Li Source Type: research