Skeletal muscle-specific forkhead box protein-O1 overexpression suppresses atherosclerosis progression in apolipoprotein E-knockout mice.
In this study, we investigated whether skeletal muscle-specific FOXO-1 overexpression suppresses the progression of atherosclerosis in ApoE-KO mice. We generated ApoE-KO/FOXO-1 mice, in which an ApoE-KO mouse was crossbred with a mouse presenting skeletal muscle-specific FOXO-1 overexpression (FOXO-1Tg). The mice were sacrificed at 20 weeks of age, and atherosclerotic plaque area and protein expression in the plaque were measured. Additionally, we measured the tumor necrosis factor α (TNFα)- induced mRNA expression in human umbilical vein endothelial cells (HUVECs), using serum collected from the FOXO-1Tg mice....
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Shimba Y, Senda R, Katayama K, Morita A, Ikeda M, Kamei Y, Miura S Tags: Biochem Biophys Res Commun Source Type: research

Hypoxia-inducible factor-2 promotes liver fibrosis in non-alcoholic steatohepatitis liver disease via the NF- κB signalling pathway.
Hypoxia-inducible factor-2 promotes liver fibrosis in non-alcoholic steatohepatitis liver disease via the NF-κB signalling pathway. Biochem Biophys Res Commun. 2021 Jan 12;540:67-74 Authors: Cai H, Bai Z, Ge RL Abstract Non-alcoholic steatohepatitis (NASH) is one of the most common chronic liver diseases. Chronic hypoxia is related to the pathogenesis of NASH. HIF-2α is the key gene for lipid metabolism, fibrosis, and inflammation in many cells. To identify the molecular mechanism through which hypoxia exposure increases the morbidity of NASH, the expression level of HIF-2α was analy...
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Cai H, Bai Z, Ge RL Tags: Biochem Biophys Res Commun Source Type: research

Mesoporous silica nanoparticles combined with AKR1C3 siRNA inhibited the growth of castration-resistant prostate cancer by suppressing androgen synthesis in  vitro and in vivo.
In this study, mesoporous silica nanoparticles (MSNs) were employed to deliver small interfering RNA targeting AKR1C3 (siAKR1C3) to downregulate AKR1C3 expression in CPRC cells. The optimal weight ratio of MSNs/siAKR1C3 was determined by a gel retardation assay. Prostate cancer cells such as VCaP cells, which intracrinally express AKR1C3, and LNCaP-AKR1C3 cells stably transfected with AKR1C3 were used to investigate the antitumour effect of MSNs-siAKR1C3. Fluorescence detection and Western blot analyses were applied to confirm the entrance of MSNs-siAKR1C3 into the cells. A SRB (Sulforhodamine B) assay was employed to asse...
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Chen J, Yang Y, Xu D, Li J, Wu S, Jiang Y, Wang C, Yang Z, Zhao L Tags: Biochem Biophys Res Commun Source Type: research

Structural insights into a new substrate binding mode of a histidine acid phosphatase from Legionella pneumophila.
Abstract MapA is a histidine acid phosphatase (HAP) from Legionella pneumophila that catalyzes the hydroxylation of a phosphoryl group from phosphomonoesters by an active-site histidine. Several structures of HAPs, including MapA, in complex with the inhibitor tartrate have been solved and the substrate binding tunnel identified; however, the substrate recognition mechanism remains unknown. To gain insight into the mechanism of substrate recognition, the crystal structures of apo-MapA and the MapAD281A mutant in complex with 5'-AMP were solved at 2.2 and 2.6 Å resolution, respectively. The structure of ...
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Guo Y, Zhou D, Zhang H, Zhang NN, Qi X, Chen X, Chen Q, Li J, Ge H, Teng YB Tags: Biochem Biophys Res Commun Source Type: research

Effect of HIF1 α on the TRPC6 channel of glomerular podocytes under chronic hypoxia.
CONCLUSIONS: TRPC6 mRNA and protein expression levels were significantly increased in podocytes under hypoxia, which may result in the increase of intracellular Ca2+. This alternation of TRPC6 may be relevant to the modulation of HIF1α. Hypoxia in podocytes can result in cytoskeleton reorganization, which further leads to podocytes injury and disfunction. PMID: 33450580 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Hu G, He Y, Li Y, Hu X, Liu S, Liao C, Zhang R, Zhou X, Sun H Tags: Biochem Biophys Res Commun Source Type: research

Hypoxia maintains the fenestration of liver sinusoidal endothelial cells and promotes their proliferation through the SENP1/HIF-1 α/VEGF signaling axis.
Hypoxia maintains the fenestration of liver sinusoidal endothelial cells and promotes their proliferation through the SENP1/HIF-1α/VEGF signaling axis. Biochem Biophys Res Commun. 2021 Jan 11;540:42-50 Authors: Qing Z, Huang H, Yang S, Lin J, Zeng Z, Duan J, Yuan B, Ming T Abstract Liver sinusoidal endothelial cells (LSECs), which play a very critical role in liver regeneration, function in hypoxic environments, but few studies have elucidated the specific mechanism. As a hypoxia-sensitive gene, Sentrin/SUMO-specific protease 1(SENP1) is upregulated in solid tumors due to hypoxia and promotes tu...
Source: Biochemical and Biophysical Research communications - January 11, 2021 Category: Biochemistry Authors: Qing Z, Huang H, Yang S, Lin J, Zeng Z, Duan J, Yuan B, Ming T Tags: Biochem Biophys Res Commun Source Type: research

Identification and heterologous expression of an isoquinolinequinone biosynthetic gene cluster from Streptomyces albus J1074.
This study uncovered a new biosynthetic machinery to produce nitrogen heterocycle natural products in microbes. PMID: 33445110 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 11, 2021 Category: Biochemistry Authors: Chai S, Cao M, Feng Z Tags: Biochem Biophys Res Commun Source Type: research

Effect of endothelin on sex-dependent regulation of tone in coronary resistance vessels.
Abstract BACKGROUND/AIMS: Sex dependent differences in coronary artery vasoregulation may be due to variations in responses to endogenous vasoactive compounds including endothelin (ET-1) and nitric oxide (NO). METHODS: Septal coronary arteries (
Source: Biochemical and Biophysical Research communications - January 11, 2021 Category: Biochemistry Authors: Kingma JG, Laher I Tags: Biochem Biophys Res Commun Source Type: research

Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2  cell injury via sponging microRNA-21-5p.
Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p. Biochem Biophys Res Commun. 2021 Jan 08;540:1-7 Authors: Wei W, Yao Y, Bi H, Xu W, Gao Y Abstract Our previous findings revealed that hsa_circ_0068,888 was markedly down-regulated in the plasma of patients with sepsis-associated acute kidney injury (AKI). However, its molecular mechanism in AKI remains unclear. Herein, we explored the role of hsa_circ_0068,888 in AKI. Human renal proximal tubular cell line HK-2 was stimulated with lipopolysaccharide (LPS) to mimic AKI in vitro...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Wei W, Yao Y, Bi H, Xu W, Gao Y Tags: Biochem Biophys Res Commun Source Type: research

Requirement of the exopolyphosphatase gene for cellular acclimation to phosphorus starvation in a cyanobacterium, Synechocystis sp. PCC 6803.
Abstract Polyphosphate, which is ubiquitous in cells in nature, is involved in a myriad of cellular functions, and has been recently focused on its metabolism related with microbial acclimation to phosphorus-source fluctuation. In view of the ecological importance of cyanobacteria as the primary producers, this study investigated the responsibility of polyphosphate metabolism for cellular acclimation to phosphorus starvation in a cyanobacterium, Synechocystis sp. PCC 6803, with the use of a disruptant (Δppx) as to the gene of exopolyphosphatase that is responsible for polyphosphate degradation. Δppx wa...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Hiyoshi T, Oyanagi K, Niki T, Fujiwara S, Sato N Tags: Biochem Biophys Res Commun Source Type: research

Uric acid inhibits HMGB1-TLR4-NF- κB signaling to alleviate oxygen-glucose deprivation/reoxygenation injury of microglia.
Uric acid inhibits HMGB1-TLR4-NF-κB signaling to alleviate oxygen-glucose deprivation/reoxygenation injury of microglia. Biochem Biophys Res Commun. 2021 Jan 08;540:22-28 Authors: Wang Q, Zhao H, Gao Y, Lu J, Xie, Yu W, He F, Liu W, Hisatome I, Yamamoto T, Wang W, Cheng J Abstract Mounting evidence has implicated inflammation in ischemia-reperfusion injury following acute ischemic stroke (AIS). Microglia remain the primary initiator and participant of brain inflammation. Emerging evidence has indicated that uric acid has promise for the treatment of AIS, but its explicit mechanisms remain elusiv...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Wang Q, Zhao H, Gao Y, Lu J, Xie, Yu W, He F, Liu W, Hisatome I, Yamamoto T, Wang W, Cheng J Tags: Biochem Biophys Res Commun Source Type: research

Spatial learning and memory impaired after infection of non-neurotropic influenza virus in BALB/c male mice.
In this study, adult mice were infected with influenza virus H1N1/PR8. Then, spontaneous behavior, body weight, expression of cytokines in brain, spatial learning ability and spatial memory ability were observed, until the complete recovery period. The results showed that cytokines in the brain were highly expressed in the convalescent phase (14 day post inoculation, dpi), especially BDNF, IBA1, CX3CL1 and CD200 were still highly expressed in the recovery phase (28 dpi). Otherwise the emotional and spatial memory ability of mice were impacted in the convalescent phase (14 dpi) and the recovery phase (28 dpi). In brief, BAL...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Gu L, Zhou Y, Wang G, Deng H, Song X, He X, Wang T, Chen X, Dai J, Li R Tags: Biochem Biophys Res Commun Source Type: research

Study on immunogenicity and antigenicity of a novel brucella multiepitope recombined protein.
In this study, the recombinant protein was used as an antigen to study the immune response produced by immunized mice, and goat serum was used to verify its diagnostic accuracy. The production of antibodies was successfully induced in the vaccinated mice. Flow cytometric analysis revealed that the percentage of CD4+, CD8+ and the CD4+/CD8+ ratios were increased by T cell subsets in mouse splenocytes, indicating that the recombinant protein induced a strong immune response had strong immunoreactivity. Using indirect ELISA, the recombinant protein correctly diagnosed positive and negative brucellosis samples. Compared with t...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Yin D, Bai Q, Li L, Xu K, Zhang J Tags: Biochem Biophys Res Commun Source Type: research

Expression of glucose transporters in human neurodegenerative diseases.
Abstract The central nervous system (CNS) plays an important role in the human body. It is involved in the receive, store and participation in information retrieval. It can use several substrates as a source of energy, however, the main source of energy is glucose. Cells of the central nervous system need a continuous supply of energy, therefore, transport of glucose into these cells is very important. There are three distinct families of glucose transporters: sodium-independent glucose transporters (GLUTs), sodium-dependent glucose cotransporters (SGLTs), and uniporter, SWEET protein. In the human brain only GLUT...
Source: Biochemical and Biophysical Research communications - January 8, 2021 Category: Biochemistry Authors: Głuchowska K, Pliszka M, Szablewski L Tags: Biochem Biophys Res Commun Source Type: research

Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN.
Abstract In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization. The results show that π-π stacking and hydrogen bonds account for the strong adsorption under neutral as well as acidic conditions. While hydrophilic and electrostatic interaction are the major modes of the peptide under alkaline condition. These different interaction mod...
Source: Biochemical and Biophysical Research communications - January 7, 2021 Category: Biochemistry Authors: Wang Y, Yu Y, Yao W, Li M, Wang P Tags: Biochem Biophys Res Commun Source Type: research

Cataract-causing mutations L45P and Y46D impair the thermal stability of γC-crystallin.
In this study, we explored the thermal stability of wild type (WT), L45P, and Y46D mutants of γC-crystallin at low and high concentrations, as well as the effect of αA-crystallin on the thermal stability of mutants. Spectroscopic experiments were used to monitor the structural changes on temperature-gradient and time-course heating process. Intermediate morphologies were determined through cryo-electron microscopy. The thermal stability of WT and mutants at concentrations ranging up to hundreds of milligrams were assessed via the UNcle multifunctional protein stability analysis system. The results showed that L...
Source: Biochemical and Biophysical Research communications - January 7, 2021 Category: Biochemistry Authors: Fu C, Xu J, Yang X, Chen X, Yao K Tags: Biochem Biophys Res Commun Source Type: research

Pro-inflammatory cytokines suppress HYBID (hyaluronan (HA) -binding protein involved in HA depolymerization/KIAA1199/CEMIP) -mediated HA metabolism in human skin fibroblasts.
Abstract In the skin, the metabolism of hyaluronan (HA) is highly regulated. Aging leads to chronic low-grade inflammation, which is characterized by elevated levels of pro-inflammatory cytokines; however, the relationship between inflammation and HA metabolism is not clear. Herein, we investigated the effects of a mixture of pro-inflammatory cytokines containing TNF-α, IL-1β, and IL-6 on HA metabolism in human skin fibroblasts. Treatment with the cytokine mixture for 24 h suppressed HA depolymerization via downregulation of HYBID (HA-binding protein involved in HA depolymerization/KIAA1199/CEMIP) ...
Source: Biochemical and Biophysical Research communications - January 7, 2021 Category: Biochemistry Authors: Sato S, Mizutani Y, Yoshino Y, Masuda M, Miyazaki M, Hara H, Inoue S Tags: Biochem Biophys Res Commun Source Type: research

Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA.
Abstract CRISPR-Cas systems, including Cas9 and Cpf1 (Cas12a), are promising tools for generating gene knockout mouse models. Unlike Cas9, Cpf1 can generate multiple crRNAs from a single concatemeric crRNA precursor, which is favorable for multiplex gene editing. Recently, a hybrid guide RNA (hgRNA) system employing both Cas9 and Cpf1 was developed for multiplex gene editing. As the crRNA of Cpf1 was linked to the 3' end of the sgRNA for Cas9, it can be split into separate guide RNAs by Cpf1. To examine whether this Cas9-Cpf1 hybrid system is suitable for multiplex gene knockouts in the mouse embryo, we generated ...
Source: Biochemical and Biophysical Research communications - January 6, 2021 Category: Biochemistry Authors: Oh SH, Lee HJ, Ahn MK, Jeon MY, Yoon JS, Jung YJ, Kim GN, Baek IJ, Kim I, Kim KM, Sung YH Tags: Biochem Biophys Res Commun Source Type: research

Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells.
This study suggests that ferritinophagy, in addition to GSH depletion, plays an important role in cystine deprivation-induced ferroptosis in glioblastoma cells. PMID: 33421769 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 6, 2021 Category: Biochemistry Authors: Hayashima K, Kimura I, Katoh H Tags: Biochem Biophys Res Commun Source Type: research

Identification of a 1-deoxy-D-xylulose-5-phosphate synthase (DXS) mutant with improved crystallographic properties.
In this report, we describe a truncated Deinococcus radiodurans 1-deoxy-D-xylulose-5-phosphate synthase (DXS) protein that retains enzymatic activity, while slowing protein degradation and showing improved crystallization properties. With modern drug-design approaches relying heavily on the elucidation of atomic interactions of potential new drugs with their targets, the need for co-crystal structures with the compounds of interest is high. DXS itself is a promising drug target, as it catalyzes the first reaction in the 2-C-methyl-D-erythritol 4-phosphate (MEP)-pathway for the biosynthesis of the universal precursors of te...
Source: Biochemical and Biophysical Research communications - January 5, 2021 Category: Biochemistry Authors: Gierse RM, Reddem ER, Alhayek A, Baitinger D, Hamid Z, Jakobi H, Laber B, Lange G, Hirsch AKH, Groves MR Tags: Biochem Biophys Res Commun Source Type: research

Functional inhibition of fatty acid binding protein 4 ameliorates impaired ciliogenesis in GCs.
Abstract Ciliogenesis is often impaired in some cancer cells, leading to acceleration of cancer phenotypes such as cell migration and proliferation. From the investigation of primary cilia of 16 gastric cancer cells (GCs), we found that GCs could be grouped into four primary cilia (PC)-positive GCs and 12 PC-negative GCs. The proliferation of the PC-positive GCs was lower than that of PC-negative GCs. To explore the role of fatty acid binding protein 4 (FABP4), which is a known oncogenic factor, in ciliogenesis, FABP4 expression and function were inhibited by transfection of cells with short interfering RNA t...
Source: Biochemical and Biophysical Research communications - January 5, 2021 Category: Biochemistry Authors: Jung Y, Cho SM, Kim S, Cheong JH, Kwon HJ Tags: Biochem Biophys Res Commun Source Type: research

Hoxb5 defines the heterogeneity of self-renewal capacity in the hematopoietic stem cell compartment.
Abstract Self-renewal and multipotency are essential functions of hematopoietic stem cells (HSCs). To maintain homeostatic hematopoiesis, functionally uniform HSCs have been thought to be an ideal cell-of-origin. Recent technological advances in the field have allowed us to analyze HSCs with single cell resolution and implicate that functional heterogeneity may exist even within the highly purified HSC compartment. However, due in part to the technical limitations of analyzing extremely rare populations and our incomplete understanding of HSC biology, neither the biological meaning of why heterogeneity exists nor ...
Source: Biochemical and Biophysical Research communications - January 5, 2021 Category: Biochemistry Authors: Sakamaki T, Kao KS, Nishi K, Chen JY, Sadaoka K, Fujii M, Takaori-Kondo A, Weissman IL, Miyanishi M Tags: Biochem Biophys Res Commun Source Type: research

What gaze direction can tell us about cognitive processes in invertebrates.
Abstract Most visually guided animals shift their gaze using body movements, eye movements, or both to gather information selectively from their environments. Psychological studies of eye movements have advanced our understanding of perceptual and cognitive processes that mediate visual attention in humans and other vertebrates. However, much less is known about how these processes operate in other organisms, particularly invertebrates. We here make the case that studies of invertebrate cognition can benefit by adding precise measures of gaze direction. To accomplish this, we briefly review the human visual attent...
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Winsor AM, Pagoti GF, Daye DJ, Cheries EW, Cave KR, Jakob EM Tags: Biochem Biophys Res Commun Source Type: research

A systems biology approach for defining the potential molecular framework of idiopathic hypereosinophilic syndrome with cutaneous involvement.
PMID: 33413980 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Wang W, Ma J, Sun X, Ba W, Meng X, Zhu Y, Leng L, Li C Tags: Biochem Biophys Res Commun Source Type: research

Discovery of a chemical compound that suppresses expression of BEX2, a dormant cancer stem cell-related protein.
Abstract Cancer stem cells (CSCs) are believed to cause cancer metastasis and recurrence. BEX2 (brain expressed X-linked gene 2) is a CSC-related gene that is expressed in dormant CSCs in cholangiocarcinoma and induces resistance against chemotherapy. The aim of the present study was to identify small compounds that have activity to inhibit BEX2 expression and result in the attenuation of CSC-related phenotypes. We screened 9600 small chemical compounds in high-throughput screening using cholangiocarcinoma cell line HuCCT1 expressing BEX2 protein fused with NanoLuc, and identified a compound, BMPP (1, 3-Benzenedio...
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Saijoh S, Nakamura-Shima M, Shibuya-Takahashi R, Ito R, Sugawara A, Yamazaki T, Imai T, Asada Y, Matsuura K, Iwai W, Wakui Y, Abue M, Kawamura S, Katayose Y, Fujimori H, Mochizuki M, Yasuda J, Yamaguchi K, Sugamura K, Satoh K, Katori Y, Tamai K Tags: Biochem Biophys Res Commun Source Type: research

Phosphine modification of proline-glycine-proline tripeptide and study of its neuroprotective properties.
CONCLUSION: Preliminary biological tests have shown that the obtained phosphine analog of the proline-glycine-proline tripeptide is involved in modulating the formation of sediments in the cellular system of proteinopathy, which may indicate their potential antiaggregatory properties. PMID: 33412416 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Alexey VV, Maxim DE, Lyudmila AA, Alexey UA, Valery SP, Kristina SN, Boris LV, Vladimir FM, Yuriy DA, Valery RV, Nikolay MF Tags: Biochem Biophys Res Commun Source Type: research

G βγ recruits and activates P-Rex1 via two independent binding interfaces.
In conclusion, we reconcile previous findings and propose a mechanistic model of P-Rex1 activation; accordingly, Gβγ recruits P-Rex1 via the Gβγ/PDZ-PDZ interface followed by a second contact involving the Gβγ/DH/PH interface to unleash P-Rex1 RacGEF activity at the plasma membrane. PMID: 33412417 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Cervantes-Villagrana RD, Beltrán-Navarro YM, García-Jiménez I, Adame-García SR, Olguín-Olguín A, Reyes-Cruz G, Vázquez-Prado J Tags: Biochem Biophys Res Commun Source Type: research

Not a piece of junk anymore: Pseudogene T04B2.1 performs non-conventional regulatory role and modulates aggregation of α- synuclein and β-amyloid proteins in C. elegans.
This study unveils the non-conventional regulatory role of pseudogene T04B2.1 with respect to effects associated with neurodegenerative diseases and encourages further studies to decipher the regulatory mechanism governed by pseudogenes. PMID: 33412418 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 4, 2021 Category: Biochemistry Authors: Shukla S, Srividya K, Nazir A Tags: Biochem Biophys Res Commun Source Type: research

Systemic effect of FHL1 on neuromuscular junction and myotube formation via insulin-like growth factor and myostatin signaling pathways.
Abstract Four-and-a-half LIM domain protein 1 (FHL1) is a member of the FHL protein family that serves as a scaffold protein to maintain normal cellular structure and function. Its mutations have been implicated in multiple muscular diseases. These FHL1 related myopathies are characterized by symptoms such as progressive muscle loss, rigid or bent spine, even cardiac or respiratory failure in some patients, which implies pathological problems not only in muscles, but also in the nervous system. Moreover, decreased FHL1 protein level has been found in patients with FHL1 mutations, indicating the protein loss-of-fun...
Source: Biochemical and Biophysical Research communications - January 2, 2021 Category: Biochemistry Authors: Wu J, Zhao K, Du Z, Chen Y, Zhang F, Jiang W, Zheng J, Wu X, Shen C, Xiao X Tags: Biochem Biophys Res Commun Source Type: research

Cephalopods: Ambassadors for rethinking cognition.
Abstract Traditional approaches in comparative cognition have a long history of focusing on a narrow range of vertebrate species. However, in recent years the range of model species has expanded. Despite this development, invertebrate taxa are still largely neglected in comparative cognition, which limits our ability to locate the origins of cognitive traits. The time has come to rethink cognition and develop a more comprehensive understanding of cognitive evolution by expanding comparative analyses to include a diverse range of invertebrate taxa. In this review, we contend that cephalopods are suitable ambassador...
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Schnell AK, Clayton NS Tags: Biochem Biophys Res Commun Source Type: research

Corrigendum to "Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF- κB signaling" [Biochem. Biophys. Res. Commun. 524 (1) (2020) 1-7].
Corrigendum to "Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF-κB signaling" [Biochem. Biophys. Res. Commun. 524 (1) (2020) 1-7]. Biochem Biophys Res Commun. 2020 Dec 31;: Authors: Yamanaka S, Sato Y, Oikawa D, Goto E, Fukai S, Tokunaga F, Takahashi H, Sawasaki T PMID: 33390248 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Yamanaka S, Sato Y, Oikawa D, Goto E, Fukai S, Tokunaga F, Takahashi H, Sawasaki T Tags: Biochem Biophys Res Commun Source Type: research

Cardiac-specific overexpression of HIF-1 α during acute myocardial infarction ameliorates cardiomyocyte apoptosis via differential regulation of hypoxia-inducible pro-apoptotic and anti-oxidative genes.
In this study, we report for the first time that myocardium-targeted nanotized HIF-1α overexpression during MI downregulates cardiomyocyte apoptosis. HIF-1α overexpression attenuates bnip3-mediated apoptosis indirectly by promoting HO-1-induced anti-oxidant response. Chromatin immunoprecipitation experiment revealed that HIF-1α overexpression in hypoxic cardiomyocytes increases binding of HIF-1α to the hypoxia-responsive element in the promoter of its target anti-oxidant gene ho-1 which is known to attenuate ROS accumulation. ROS accumulation in hypoxic cardiomyocytes causes cysteine oxidation of th...
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Datta Chaudhuri R, Banik A, Mandal B, Sarkar S Tags: Biochem Biophys Res Commun Source Type: research

Skip participates in formation and lipid metabolism of beige adipose and might mediate the effects of SIRT1 activator BTM-0512 on beige remodeling.
In conclusion, our study demonstrated that Skip might play an important role in the beige remodeling of white adipocytes as well as lipid metabolism of beige adipose. PMID: 33388413 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Liao ZM, Li AN, Cai Y, Chen JJ, Xu Y, Sui LH, Wang JL, Jin P, Wang KS, Yang ZC Tags: Biochem Biophys Res Commun Source Type: research

Graft-versus-host disease develops in mice transplanted with lymphocyte-depleted bone marrow cells from signal-transducing adaptor protein-2 transgenic mice.
Abstract Graft-versus-host disease (GVHD) is the most frequent complication after allogeneic hematopoietic stem cell transplantation (HSCT), and is one of the major causes of non-relapse mortality. Transferred mature lymphocytes are thought to be responsible for GVHD based on the findings that mice transplanted with lymphocyte-depleted bone marrow (BM) cells from MHC-mismatched donors do not develop GVHD. However, we found that overexpression of signal-transducing adaptor protein (STAP)-2 in lymphoid cells could induce GVHD after lymphocyte-depleted BM transplantation. To examine the function of STAP-2, which has ...
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Saito H, Ichii M, Toda J, Kitai Y, Muromoto R, Kashiwakura JI, Saitoh K, Tanimura A, Yokota T, Shibayama H, Matsuda T, Oritani K, Kanakura Y, Hosen N Tags: Biochem Biophys Res Commun Source Type: research

LSR promotes epithelial ovarian cancer cell survival under energy stress through the LKB1-AMPK pathway.
In this study, we evaluated liver kinase B1 (LKB1) mediated AMP-activated protein kinase (AMPK) activity and investigated the effect of LSR on EOC cell survival under energy stress. LSR increased the levels of phospho-AMPKα at Thr172 and phospho-acetyl-CoA carboxylase (ACC) at Ser79 via LKB1-AMPK pathway in glucose deprivation in vitro. The increase of P-AMPKα (Thr172) and P-ACC (Ser79) was also detected in tumor microenvironment in vivo. Meanwhile, LSR promoted LKB1 localization at the cell membrane of EOC cells. By cell survival analysis, LSR attenuated glucose deprivation-induced cell death in EOC ...
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Takahashi Y, Serada S, Ohkawara T, Fujimoto M, Hiramatsu K, Ueda Y, Kimura T, Takemori H, Naka T Tags: Biochem Biophys Res Commun Source Type: research

Combination of talaporfin photodynamic therapy and Poly (ADP-Ribose) polymerase (PARP) inhibitor in gastric cancer.
Abstract Photodynamic therapy (PDT) utilizes photochemical reactions induced by a photosensitizer and light in the target tissue and is used to treat various cancers. There is a high degree of anticipation of success regarding the application of PDT with talaporfin (photosensitizer) for gastric cancer. Olaparib is an oral inhibitor of Poly (ADP-Ribose) polymerase (PARP) and has demonstrated optimal efficacy and clinical activity in trials. Therefore, the aim of the present study was to investigate the efficacy of talaporfin PDT combined with olaparib for gastric cancer. MKN45, a gastric cancer cell line, was incub...
Source: Biochemical and Biophysical Research communications - December 31, 2020 Category: Biochemistry Authors: Tanaka M, Sasaki M, Suzuki T, Nishie H, Kataoka H Tags: Biochem Biophys Res Commun Source Type: research

Dynamic coupling analysis on plant sesquiterpene synthases provides leads for the identification of product specificity determinants.
Abstract Sesquiterpene synthases catalyse cyclisation of farnesyl pyrophosphate to produce diverse sesquiterpenes. Despite utilising the same substrate and exhibiting significant sequence and structural homology, these enzymes form different products. Previous efforts were based on identifying the effect of divergent residues present at the catalytic binding pocket on the product specificity of these enzymes. However, the rationales deduced for the product specificity from these studies were not generic enough to be applicable to other phylogenetically distant members of this family. To address this problem, we ha...
Source: Biochemical and Biophysical Research communications - December 30, 2020 Category: Biochemistry Authors: Singh S, Thulasiram HV, Sengupta D, Kulkarni K Tags: Biochem Biophys Res Commun Source Type: research

Engineering of an oleate hydratase for efficient C10-Functionalization of oleic acid.
Abstract Oleate hydratase catalyzes the hydration of unsaturated fatty acids, giving access to C10-functionalization of oleic acid. The resultant 10-hydroxystearic acid is a key material for the synthesis of many biomass-derived value-added products. Herein, we report the engineering of an oleate hydratase from Paracoccus aminophilus (PaOH) with significantly improved catalytic efficiency (from 33 s-1 mM-1 to 119 s-1 mM-1), as well as 3.4 times increased half-life at 30 °C. The structural mechanism regarding the impact of mutations on the improved catalytic activity and thermostability...
Source: Biochemical and Biophysical Research communications - December 30, 2020 Category: Biochemistry Authors: Sun QF, Zheng YC, Chen Q, Xu JH, Pan J Tags: Biochem Biophys Res Commun Source Type: research

A novel long non-coding RNA PCLN16 facilitates androgen receptor signaling in prostate cancer.
Abstract The prostate cancer (PCa) poses serious threat to men's health. The androgen receptor (AR) is essential for normal prostate development and prostate cancer progression. We identified a novel lncRNA PCLN16 which is significantly correlated with AR signaling during prostate cancer progression. The AR-regulated PCLN16 was abundantly overexpressed in localized or metastatic prostate cancer tissues and AR-dependent cell lines. PCLN16 silence suppressed AR signaling and tumor growth. PCLN16 interacted with Huntingtin interacting protein 1 (HIP1) transcript to reduce HIP1 degradation. Therefore, PCLN16 could aug...
Source: Biochemical and Biophysical Research communications - December 30, 2020 Category: Biochemistry Authors: Shi Z, Chen J, Wumaner A, Li M, Liang C, Li M Tags: Biochem Biophys Res Commun Source Type: research

Novel tankyrase inhibitors suppress TDP-43 aggregate formation.
Abstract Transactive response DNA-binding protein of 43 kDa (TDP-43) abnormally forms aggregates in certain subtypes of frontotemporal lobar degeneration (FTLD) and in amyotrophic lateral sclerosis (ALS). The pathological forms of TDP-43 have reported to be associated with poly(ADP-ribose) (PAR), which regulates the properties of these aggregates. A recent study has indicated that tankyrase, a member of the PAR polymerase (PARP) family, regulates pathological TDP-43 formation under conditions of stress, and tankyrase inhibitors suppress TDP-43 aggregate formation and cytotoxicity. Since we reported the develo...
Source: Biochemical and Biophysical Research communications - December 30, 2020 Category: Biochemistry Authors: Tanji K, Mori F, Shirai F, Fukami T, Seimiya H, Utsumi J, Kakita A, Wakabayashi K Tags: Biochem Biophys Res Commun Source Type: research

Identification of novel compounds that inhibit SnRK2 kinase activity by high-throughput screening.
Abstract Abscisic acid (ABA) is a major phytohormone that regulates abiotic stress responses and development. SNF1-rerated protein kinase 2 (SnRK2) is a key regulator of ABA signaling. To isolate compounds which directly affect SnRK2 activity, we optimized a fluorescence-based system for high-throughput screening (HTS) of SnRK2 kinase regulators. Using this system, we screened a chemical library consisting of 16,000 compounds and identified ten compounds (INH1-10) as potential SnRK2 inhibitors. Further characterization of these compounds by in vitro phosphorylation assays confirmed that three of the ten compo...
Source: Biochemical and Biophysical Research communications - December 29, 2020 Category: Biochemistry Authors: Matsuoka S, Sato K, Maruki-Imamura R, Noutoshi Y, Okabe T, Kojima H, Umezawa T Tags: Biochem Biophys Res Commun Source Type: research

D-Serine inhibits the attachment and biofilm formation of methicillin-resistant Staphylococcus aureus.
CONCLUSION: D-Ser inhibits the attachment and biofilm formation of MRSA by reducing the expression of the related genes. Also, the administration of D-Ser reduces the severity of catheter infection in the mouse model. Therefore, D-Ser may be a promising therapeutic option for MRSA as well as E. coli infection. PMID: 33385805 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Iwata Y, Sakai N, Yoneda I, Senda Y, Sakai-Takemori Y, Oshima M, Nakagawa-Yoneda S, Ogura H, Sato K, Minami T, Kitajima S, Toyama T, Yamamura Y, Miyagawa T, Hara A, Shimizu M, Furuichi K, Matsushima K, Wada T Tags: Biochem Biophys Res Commun Source Type: research

miR-30b-5p modulate renal epithelial-mesenchymal transition in diabetic nephropathy by directly targeting SNAI1.
CONCLUSION: These findings suggest that miR-30b-5p play a protective role by targeting SNAI1 in renal EMT in DN. PMID: 33383483 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Wang Y, Liu Y, Zhang L, Bai L, Chen S, Wu H, Sun L, Wang X Tags: Biochem Biophys Res Commun Source Type: research

ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase.
In this study, we show that ADAM10 and ADAM17 are degraded through the lysosomal pathway. The lysosomal cysteine protease, AEP, plays an important role in the degradation of ADAM10/17. AEP directly cleaves ADAM10/17. Knockout of AEP increases the content of ADAM10/17 in the brain. Given the protective role of ADAM10 and ADAM17 against AD, AEP-mediated degradation of ADAM10/17 may be involved in the pathogenesis of AD. PMID: 33383559 [PubMed - as supplied by publisher] (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Zhang X, Tang L, Zhang Z Tags: Biochem Biophys Res Commun Source Type: research

BTF3 promotes stemness and inhibits Type ⅠInterferon signaling pathway in triple-negative breast cancer.
BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer. Biochem Biophys Res Commun. 2020 Dec 28;537:22-28 Authors: Wang H, Gao L, Qi M, Su P, Xiong X, Zhao J, Hu J, Han B Abstract Triple-negative breast cancer (TNBC) is a major challenge in clinical practice due to its aggressiveness and lack of targeted treatment. Cancer stem-like traits contribute to tumorigenesis and immune privilege of TNBC. However, the relationship of stemness and immunosurveillance remains unclear. Here, we demonstrate that BTF3 expression is related with stem-like properties in...
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Wang H, Gao L, Qi M, Su P, Xiong X, Zhao J, Hu J, Han B Tags: Biochem Biophys Res Commun Source Type: research

Swelling-activated ClC-3 activity regulates prostaglandin E2 release in human OUMS-27 chondrocytes.
In this study, we reveal that in human OUMS-27 chondrocytes, ICl,swell can be elicited by hypoosmotic stimulation (180 mOsm) and be inhibited by classical Cl- channel blockers, 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS) and niflumic acid, and be attenuated by siRNA knockdown of ClC-3. Our molecular analyses revealed that ClC-3A is expressed as a major splice variant in both human articular chondrocytes and OUMS-27 cells. The onset and early phase of RVD following hypoosmotic stress in OUMS-27 cells were affected by DIDS and ClC-3 knockdown. Hypoosmotic stimulation caused Ca2+ influx and subsequent re...
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Yamada S, Suzuki Y, Bernotiene E, Giles WR, Imaizumi Y, Yamamura H Tags: Biochem Biophys Res Commun Source Type: research

Peptide OM-LV20 exerts neuroprotective effects against cerebral ischemia/reperfusion injury in rats.
Abstract Ischemia/reperfusion (I/R) is a common injury leading to ischemic stroke. At present, I/R treatment remains limited, highlighting the urgent need for the discovery and development of new protective drugs for brain injury. Here, we investigated the neuroprotective effects of short peptide OM-LV20 previously identified from amphibian against I/R rats. Results showed that intraperitoneal administration of OM-LV20 (20 ng/kg) significantly reduced infarct area formation, improved behavioral abnormalities, and protected cortical and hippocampal neurons against death caused by I/R. Moreover, the underlying ...
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Yin S, Yang M, Li Y, Li S, Fu Z, Liu N, Wang Y, Hu Y, Xie C, Shu L, Pang A, Gu Y, Wang Y, Sun J, Yang X Tags: Biochem Biophys Res Commun Source Type: research

Cataract formation in transgenic HO-1 G143H mutant mice: Involvement of oxidative stress and endoplasmic reticulum stress.
Abstract Oxidative stress and endoplasmic reticulum (ER) stress are the key contributing factors for cataract progression. In our previous studies, we demonstrated that the nuclear factor erythroid 2-like-2 (Nrf-2)/heme oxygenase-1 (HO-1)/carbon monoxide (CO) axis protects lens epithelial cells (LECs) against oxidants and ER stress. In the present study, transgenic FVB/N mice overexpressing the negative dominant mutant HO-1 G143H (TgHO-1 G143H) were generated to evaluate the crosstalk among HO-1, oxidative stress and ER stress in maintaining lens transparency. Slit-lamp examination revealed that nuclear cataracts ...
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Huang Y, Ye Z, Yin Y, Ma T, Zhang Q, Shang K, Chen W, Li Z Tags: Biochem Biophys Res Commun Source Type: research

Exogenous hydrogen sulfide inhibits neutrophils extracellular traps formation via the HMGB1/TLR4/p-38 MAPK/ROS axis in hyperhomocysteinemia rats.
Abstract Hydrogen sulfide (H2S) prevents platelet activation and neutrophils extracellular traps (NETs) formation. However, the mechanism of sodium hydrosulfide (NaHS, a donor that produces H2S) inhibits the formation of NETs in hyperhomocysteinemia (HHcy) rats has not been previously investigated. In the experiment, the expressions of HMGB1 of platelets, the expressions of TLR4, PAD4 and the phosphor-p38 of neutrophils were measured. The NETs formations, the concentration of DNA in the serum and the culture solution of cultured neutrophils which was stimulated by platelet-rich plasma (PRP) were tested. Additional...
Source: Biochemical and Biophysical Research communications - December 28, 2020 Category: Biochemistry Authors: Zhao X, Zhang L, Liu X, Zhao Z, Zhong X, Wang Y Tags: Biochem Biophys Res Commun Source Type: research

Odour priming of a mosquito-specialist predator's vision-based detouring decisions.
Abstract A capacity to execute long detours that are planned ahead of time has cognitive implications pertaining to reliance on internal representation. Here we investigate the detouring behaviour of Evarcha culicivora, an East African salticid spider that specializes at preying on blood-carrying mosquitoes. The findings from our experiments are the first evidence of a salticid making detouring plans based on whether the path chosen leads to more preferred instead of less preferred prey, as well as the first evidence of olfactory priming effects on motivation and selective attention in the context of detouring. Te...
Source: Biochemical and Biophysical Research communications - December 26, 2020 Category: Biochemistry Authors: Cross FR, Jackson RR Tags: Biochem Biophys Res Commun Source Type: research