Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells
In this study, we sought to examine the effects of Kif26b loss-of-function on adipogenic differentiation of murine C3H10T1/2 multipotent stromal cells. In vitro loss-of-function studies demonstrated that Kif26b knockdown by lentivirus mediated shRNA markedly dampened the differentiation potential of C3H10T1/2 cells to adipocytes and suppressed the expression of adipogenesis-related genes e.g., Pparg, C/ebpα, Fabp4 and Adipoq. Analysis of cell cycle revealed that Kif26b knockdown resulted in elevated expression of cyclins (Ccnd1, Ccnb1, Ccna2) along with rapid cell cycle progression from G0/G1 to S and G2 phases. Mech...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Mingming Yan Lei Cai Xin Duan Muhammad Farooq Rai Source Type: research

All-trans retinoic acid inhibits HCV replication by downregulating core levels via E6AP-mediated proteasomal degradation
Biochem Biophys Res Commun. 2022 Jan 17;594:15-21. doi: 10.1016/j.bbrc.2022.01.052. Online ahead of print.ABSTRACTHere, we found that all-trans retinoic acid (ATRA), the most biologically active metabolite of vitamin A, strengthens the anti-viral defense mechanism of E6-associated protein (E6AP) that downregulates hepatitis C virus (HCV) Core levels via ubiquitin-dependent proteasomal degradation. For this effect, ATRA downregulated both protein and enzyme activity levels of DNA methyltransferase 1 and 3b and activated E6AP expression via promoter hypomethylation in HepG2 cells but not in Hep3B cells, in which p53 was abse...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Hye-Kyoung Lee Hyunyoung Yoon Kyung Lib Jang Source Type: research

Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period
In this study, we aimed to characterize the lncRNA expression profile in conceptuses during peri-implantation periods in sheep. We analyzed the RNA-sequence data of ovine conceptuses and endometria obtained from pregnant animals on days 15, 17, 19 and 21 (day 0 = day of estrus, n = 3 or 4/day). We predicted the protein coding ability of the assembled transcripts to identify the lncRNA candidates. This analysis identified 8808 lncRNAs, 3423 of which were novel lncRNAs. Gene ontology analysis revealed that lncRNA target genes were enriched for biological processes involved in the respiratory electron transport chain (RETC). ...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Yuta Matsuno Kazuya Kusama Kazuhiko Imakawa Source Type: research

A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases
Biochem Biophys Res Commun. 2022 Jan 15;594:31-37. doi: 10.1016/j.bbrc.2022.01.051. Online ahead of print.ABSTRACTThe main pathological characteristics of demyelinating diseases are central nervous system (CNS) myelin damage, and the differentiation of oligodendrocyte precursor cells is the therapeutic target of myelin repair. Previous studies have found that a large number of platelet-derived growth factor receptor α(PDGFRα) positive oligodendrocyte progenitor cells (OPCs) accumulate in the lesion area of myelin injury, and differentiation is blocked. However, the therapeutic effects of drugs currently used cl...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Yun Xiao Yuan Zhang Yu-Han Gao Zhuo-Hua Zhao Jin He Rui Gao Yu-Xin Guo Li-Bin Wang Xing Li Source Type: research

Stromal cell-derived factor-1 exerts opposing roles through CXCR4 and CXCR7 in angiotensin II-induced adventitial remodeling
Biochem Biophys Res Commun. 2022 Jan 13;594:38-45. doi: 10.1016/j.bbrc.2022.01.030. Online ahead of print.ABSTRACTRecent studies have emphasized the role of vascular adventitia inflammation and immune response in hypertension. It has been reported that stromal cell-derived factor-1 (SDF-1) plays various biological functions through its receptors C-X-C motif chemokine receptor 4 (CXCR4) and CXCR7 in tumor growth and tissue repair. However, it is unclear that whether SDF-1/CXCR4/CXCR7 axis is involved in hypertensive vascular remodeling. In the present study, the involvement of SDF-1/CXCR4/CXCR7 axis was evaluated with lenti...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Bei Song Dongrui Chen Zixiong Liu Yuwen Cheng Zebei Zhang Weiqing Han Ruiyan Zhang Yanchun Gong Source Type: research

Crystal structure of the plant feruloyl-coenzyme A monolignol transferase provides insights into the formation of monolignol ferulate conjugates
Biochem Biophys Res Commun. 2022 Jan 13;594:8-14. doi: 10.1016/j.bbrc.2022.01.037. Online ahead of print.ABSTRACTLignin is a highly complex phenolic polymer which is essential for plants, but also makes it difficult for industrial processing. Engineering lignin by introducing relatively labile linkages into the lignin backbone can render it more amenable to chemical depolymerization. It has been reported that introducing a feruloyl-coenzyme A monolignol transferase from Angelica sinensis (AsFMT) into poplar could incorporate monolignol ferulate conjugates (ML-FAs) into lignin polymers, suggesting a promising way to manipul...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Xi Liu Shuliu Dai Yu Zhou Jinyue Liu Dong Li Jun Zhang Yushan Zhu Qiao Zhao Yue Feng Yi Zhang Source Type: research

Depression-like symptoms due to Dcf1 deficiency are alleviated by intestinal transplantation of Lactobacillus murine and Lactobacillus reuteri
Biochem Biophys Res Commun. 2022 Jan 12;593:137-143. doi: 10.1016/j.bbrc.2022.01.026. Online ahead of print.ABSTRACTDepression, characterized by low mood, is a complex mental disorder that is a serious threat to human health. Depression is thought to be caused by a combination of genetic, environmental and psychological factors. However, the pathophysiology of depression remains unclear. In the present study, we found that Dcf1 knockout (KO) mice had depression-like symptoms and disruptive changes in gamma-aminobutyric acid (GABA) concentration and GABA receptor expression were found in the hippocampus of Dcf1 KO and WT mi...
Source: Biochemical and Biophysical Research communications - January 23, 2022 Category: Biochemistry Authors: Haicong Zhou Shibo Zhang Xueqi Zhang Hong Zhou Tieqiao Wen Jiao Wang Source Type: research

The USP18-FBXO6 axis maintains the malignancy of ovarian cancer
Biochem Biophys Res Commun. 2022 Jan 11;593:101-107. doi: 10.1016/j.bbrc.2022.01.020. Online ahead of print.ABSTRACTUbiquitin-specific protease 18 (USP18) is a deubiquitinating enzyme that reverses the post-translational modification of target proteins by ISG15 or ubiquitin, and is involved in a variety of cellular processes, including signal transduction, viral infection, and cancer development. Although high levels of USP18 mRNA have been observed in several types of cancer, its pathological significance in ovarian cancer (OV) is still elusive. Here, by integrating the Cancer Genome Atlas (TCGA), Gene Expression Omnibus ...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Guanchu Li Wen Shi Yuxin Xu Kun Li Zefeng Chen Mingxiao Lv Jinyu Lv Teng Qiu Qilan Qian Jing Ji Wei Liu Bin Liu Yi Zhao Source Type: research

Differential growth inhibition, cell cycle arrest and apoptosis of MCF-7 and MDA-MB-231  cells to holocarboxylase synthetase suppression
Biochem Biophys Res Commun. 2022 Jan 14;593:108-115. doi: 10.1016/j.bbrc.2022.01.049. Online ahead of print.ABSTRACTHolocarboxylase synthetase (HLCS) catalyzes the covalent attachment of biotin onto the biotin-dependent carboxylases. Recent studies have shown that HLCS is over-expressed in breast cancer patients. Here we investigated the functional roles of free biotin and HLCS in supporting growth and migration of breast cancer cell lines. Depletion of biotin from culture medium markedly reduced biotinylation of the two most abundant biotin-carboxylases, acetyl-CoA carboxylase and pyruvate carboxylase. This was accompanie...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Siraprapa Siritutsoontorn Witchuda Sukjoi Steven W Polyak Chareeporn Akekawatchai Sarawut Jitrapakdee Source Type: research

A novel ribosome-dimerization protein found in the hyperthermophilic archaeon Pyrococcus furiosus using ribosome-associated proteomics
Biochem Biophys Res Commun. 2022 Jan 13;593:116-121. doi: 10.1016/j.bbrc.2022.01.043. Online ahead of print.ABSTRACTRibosome dimerization is one of the bacterial events that suppresses protein synthesis in the stationary phase. Protein factors responsible for ribosome dimerization in bacteria are well characterized, whereas no information is available for the corresponding factors in archaeal and eukaryotic cells. Here we describe a protein found among the ribosome-associated proteins which dimerizes the 30S ribosomal subunit of the archaeon Pyrococcus furiosus. The ribosome-associated proteins were prepared by high-salt w...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Chiaki Yaeshima Natsumi Murata Sonoko Ishino Ikuko Sagawa Kosuke Ito Toshio Uchiumi Source Type: research

The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein
Biochem Biophys Res Commun. 2022 Jan 13;593:122-128. doi: 10.1016/j.bbrc.2022.01.019. Online ahead of print.ABSTRACTChemoresistance is a major obstacle faced by oesophageal cancer patients and is synonymous with a poor prognosis. MCL1 is a pivotal member of the anti-apoptotic Bcl-2 protein family, which has been found to play an important role in cell survival, proliferation, differentiation and chemoresistance. Thus, it might be an ideal target for treating oesophageal cancer patients. Although it is known that MCL1 is degraded via the ubiquitin-proteasome system, the deubiquitylating enzyme (DUB) responsible for stabiliz...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Jinan Feng Pengyang Liu Xiaonan Li Dian Zhang Hanbin Lin Zhenzhu Hou Cairu Guo Yujie Niu Bingyu Dai Ouyang Wang Min Qi Huirui Wang Haitao Zhou Source Type: research

Neonatal exposure to sevoflurane impairs preference for social novelty in C57BL/6 female mice at early-adulthood
In conclusion, sevoflurane anesthesia in neonatal period could disturb development of social memory and impair preference for social novelty in female mice at early-adulthood, with the potential mechanism of decreasing PSD95 expression in the hippocampus of C57BL/6 mice.PMID:35063768 | DOI:10.1016/j.bbrc.2022.01.022 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Huayue Liu Xiaowen Meng Yixuan Li Shiwen Chen Yumeng Ji Shaoyong Song Fuhai Ji Xin Jin Source Type: research

How tetraspanin-mediated cell entry of SARS-CoV-2 can dysregulate the shedding of the ACE2 receptor by ADAM17
Biochem Biophys Res Commun. 2022 Jan 15;593:52-56. doi: 10.1016/j.bbrc.2022.01.038. Online ahead of print.ABSTRACTCOVID-19, the respiratory infection caused by the novel coronavirus SARS-CoV-2, presents a clinical picture consistent with the dysregulation of many of the pathways mediated by the metalloprotease ADAM17. ADAM17 is a sheddase that plays a key role in the modulation of ACE2, the receptor which also functions as the point of attachment leading to cell entry by the virus. This work investigates the possibility that ADAM17 dysregulation and attachment of the SARS-CoV-2 virion to the ACE2 receptor are linked events...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Eamonn F Healy Source Type: research

Atorvastatin ameliorates depressive behaviors via regulation of α7nAChR expression by PI3K/Akt-BDNF pathway in mice
This study was to investigate the effect of AV treatment on depressive behaviors. Herein, we show that AV treatment had antidepressant-like effect in physiological conditions and antidepressant effect in depressive state which depended on α7 nicotinic acetylcholine receptor (α7nAChR) expression in the ventral hippocampus (vHPC), but not α4β2 nicotinic acetylcholine receptor (α4β2nAchR) expression in vHPC, nor the α7nAChR and α4β2nAchR expression in dorsal hippocampus (dHPC). By using MLA, a selective α7nAChR antagonist, we investigated the role of α7nAChR in AV...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Chao Yang Huan-Yu Ni Jia-Jie Yin Ting Zhou Qiu-Xiang Gu Ting-Ting Chen Cheng-Yun Cai Source Type: research

Icariin inhibits isoproterenol-induced cardiomyocyte hypertropic injury through activating autophagy via the AMPK/mTOR signaling pathway
In conclusion, these findings indicated that ICA attenuated cardiomyocyte hypertrophy induced by ISO and prevented cell injury, and the specific mechanism was mediated by AMPK/mTOR pathway to enhance autophagy and reduce autophagy-related cardiomyocyte apoptosis.PMID:35063771 | DOI:10.1016/j.bbrc.2022.01.029 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Lin Hu Ziyuan Wang Huan Li Jiarui Wei Fengyue Tang Qing Wang Jing Wang Xiaoqiao Zhang Qiufang Zhang Source Type: research

Identification of a novel target site for ATP-independent ERK2 inhibitors
Biochem Biophys Res Commun. 2022 Jan 15;593:73-78. doi: 10.1016/j.bbrc.2022.01.035. Online ahead of print.ABSTRACTExtracellular signal-regulated kinase 2 (ERK2) controls vital physiological processes involving proliferation and differentiation and is a drug target molecule for many diseases such as cancers. In silico screening focusing on an allosteric site that plays a crucial role in substrate anchoring conferred an ERK2 inhibitor (compound 1). However, a competitive binding assay indicated that compound 1 did not bind to the allosteric site. Here, the crystal structure of ERK2 in complex with compound 1 revealed a novel...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Mayu Yoshida Haruna Nagao Hajime Sugiyama Masaaki Sawa Takayoshi Kinoshita Source Type: research

Juggling with fluorescent proteins: Spectrum and structural changes of the mCardinal2 variants
Biochem Biophys Res Commun. 2022 Jan 14;593:79-83. doi: 10.1016/j.bbrc.2022.01.044. Online ahead of print.ABSTRACTmCardinal2 is a red fluorescent protein developed through the designs of mKate, mNeptune and mCardinal. Fluorescence spectrums of mCardinal2 and its five mutants (T143C, T143G, C158A, C158D and M160E) were measured with their quantum yields. C158A and C158D increased brightness with slight changes in fluorescence spectrums while T143C, T143G and M160E decreased brightness with blue shift in fluorescence spectrums, which resulted in green, cyan and green fluorescent proteins respectively. Crystal structures of a...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Tae-Yeon Kim Tae-Sung Yoon Sunghyun Kang Muhammad Afzal Source Type: research

Identification of ERCC8 as a novel cisplatin-resistant gene in esophageal cancer based on genome-scale CRISPR/Cas9 screening
Biochem Biophys Res Commun. 2022 Jan 13;593:84-92. doi: 10.1016/j.bbrc.2022.01.033. Online ahead of print.ABSTRACTEsophageal cancer (ESCA) is one of the most common malignant tumors of the digestive system worldwide. As a first-line drug for chemotherapy, cisplatin resistance is the major obstacle in the successful treatment of esophageal cancer. Previous studies largely failed to identify the key genes associated with cisplatin resistance. Hence, the aim of this study was to screen the cisplatin resistance-related genes of esophageal cancer using CRISPR/Cas9 gene-editing technology and Brunello iBar library. Of note, we i...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Xue Sui Xiaolong Tang Xi Wu Yongshuo Liu Source Type: research

PDGF-BB is involved in phosphate regulation via the phosphate transporters in human neuroblastoma SH-SY5Y cells
Biochem Biophys Res Commun. 2022 Jan 14;593:93-100. doi: 10.1016/j.bbrc.2022.01.045. Online ahead of print.ABSTRACTInorganic phosphate (Pi) is the second most abundant inorganic ion in the body. Since abnormalities in Pi metabolism are risk factors for various diseases, serum Pi levels are strictly controlled. Type-III sodium-dependent Pi transporters, PiT-1 (encoded by SLC20A1) and PiT-2 (encoded by SLC20A2), are distributed throughout the tissues of the body, including the central nervous system, and are known to be responsible for extracellular to intracellular Pi transport. Platelet-derived growth factor (PDGF) is a ma...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Naoko Takase Masatoshi Inden Yuto Murayama Ayane Mishima Hisaka Kurita Isao Hozumi Source Type: research

Changes of flavin-containing monooxygenases and trimethylamine-N-oxide may be involved in the promotion of non-alcoholic fatty liver disease by intestinal microbiota metabolite trimethylamine
In this study, we explored the role of TMA in the process of NAFLD. The human NAFLD liver puncture data set GSE89632 and rat TMAO gene chip GSE135856 was downloaded for gene differential expression analysis. Besides, oleic acid (OA) combined with palmitate were used to establish high-fat cell model. TMA, TMAO and FMO1-siRNA were used to stimulate L02 cells. Contents of free fatty acid (FFA), triglyceride (TG), TMAO, FMO1 and unfolded protein response (UPR) related proteins GRP78, XBP1, Derlin-1 were detected. Our results showed that FMO1 and PEG10 were important in the progression of NAFLD. Immunohistochemistry showed that...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Chunxia Shi Maohua Pei Yao Wang Qian Chen Pan Cao Luyi Zhang Jin Guo Wei Deng Luwen Wang Xun Li Zuojiong Gong Source Type: research

The USP18-FBXO6 axis maintains the malignancy of ovarian cancer
Biochem Biophys Res Commun. 2022 Jan 11;593:101-107. doi: 10.1016/j.bbrc.2022.01.020. Online ahead of print.ABSTRACTUbiquitin-specific protease 18 (USP18) is a deubiquitinating enzyme that reverses the post-translational modification of target proteins by ISG15 or ubiquitin, and is involved in a variety of cellular processes, including signal transduction, viral infection, and cancer development. Although high levels of USP18 mRNA have been observed in several types of cancer, its pathological significance in ovarian cancer (OV) is still elusive. Here, by integrating the Cancer Genome Atlas (TCGA), Gene Expression Omnibus ...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Guanchu Li Wen Shi Yuxin Xu Kun Li Zefeng Chen Mingxiao Lv Jinyu Lv Teng Qiu Qilan Qian Jing Ji Wei Liu Bin Liu Yi Zhao Source Type: research

Differential growth inhibition, cell cycle arrest and apoptosis of MCF-7 and MDA-MB-231  cells to holocarboxylase synthetase suppression
Biochem Biophys Res Commun. 2022 Jan 14;593:108-115. doi: 10.1016/j.bbrc.2022.01.049. Online ahead of print.ABSTRACTHolocarboxylase synthetase (HLCS) catalyzes the covalent attachment of biotin onto the biotin-dependent carboxylases. Recent studies have shown that HLCS is over-expressed in breast cancer patients. Here we investigated the functional roles of free biotin and HLCS in supporting growth and migration of breast cancer cell lines. Depletion of biotin from culture medium markedly reduced biotinylation of the two most abundant biotin-carboxylases, acetyl-CoA carboxylase and pyruvate carboxylase. This was accompanie...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Siraprapa Siritutsoontorn Witchuda Sukjoi Steven W Polyak Chareeporn Akekawatchai Sarawut Jitrapakdee Source Type: research

A novel ribosome-dimerization protein found in the hyperthermophilic archaeon Pyrococcus furiosus using ribosome-associated proteomics
Biochem Biophys Res Commun. 2022 Jan 13;593:116-121. doi: 10.1016/j.bbrc.2022.01.043. Online ahead of print.ABSTRACTRibosome dimerization is one of the bacterial events that suppresses protein synthesis in the stationary phase. Protein factors responsible for ribosome dimerization in bacteria are well characterized, whereas no information is available for the corresponding factors in archaeal and eukaryotic cells. Here we describe a protein found among the ribosome-associated proteins which dimerizes the 30S ribosomal subunit of the archaeon Pyrococcus furiosus. The ribosome-associated proteins were prepared by high-salt w...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Chiaki Yaeshima Natsumi Murata Sonoko Ishino Ikuko Sagawa Kosuke Ito Toshio Uchiumi Source Type: research

The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein
Biochem Biophys Res Commun. 2022 Jan 13;593:122-128. doi: 10.1016/j.bbrc.2022.01.019. Online ahead of print.ABSTRACTChemoresistance is a major obstacle faced by oesophageal cancer patients and is synonymous with a poor prognosis. MCL1 is a pivotal member of the anti-apoptotic Bcl-2 protein family, which has been found to play an important role in cell survival, proliferation, differentiation and chemoresistance. Thus, it might be an ideal target for treating oesophageal cancer patients. Although it is known that MCL1 is degraded via the ubiquitin-proteasome system, the deubiquitylating enzyme (DUB) responsible for stabiliz...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Jinan Feng Pengyang Liu Xiaonan Li Dian Zhang Hanbin Lin Zhenzhu Hou Cairu Guo Yujie Niu Bingyu Dai Ouyang Wang Min Qi Huirui Wang Haitao Zhou Source Type: research

Neonatal exposure to sevoflurane impairs preference for social novelty in C57BL/6 female mice at early-adulthood
In conclusion, sevoflurane anesthesia in neonatal period could disturb development of social memory and impair preference for social novelty in female mice at early-adulthood, with the potential mechanism of decreasing PSD95 expression in the hippocampus of C57BL/6 mice.PMID:35063768 | DOI:10.1016/j.bbrc.2022.01.022 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Huayue Liu Xiaowen Meng Yixuan Li Shiwen Chen Yumeng Ji Shaoyong Song Fuhai Ji Xin Jin Source Type: research

How tetraspanin-mediated cell entry of SARS-CoV-2 can dysregulate the shedding of the ACE2 receptor by ADAM17
Biochem Biophys Res Commun. 2022 Jan 15;593:52-56. doi: 10.1016/j.bbrc.2022.01.038. Online ahead of print.ABSTRACTCOVID-19, the respiratory infection caused by the novel coronavirus SARS-CoV-2, presents a clinical picture consistent with the dysregulation of many of the pathways mediated by the metalloprotease ADAM17. ADAM17 is a sheddase that plays a key role in the modulation of ACE2, the receptor which also functions as the point of attachment leading to cell entry by the virus. This work investigates the possibility that ADAM17 dysregulation and attachment of the SARS-CoV-2 virion to the ACE2 receptor are linked events...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Eamonn F Healy Source Type: research

Atorvastatin ameliorates depressive behaviors via regulation of α7nAChR expression by PI3K/Akt-BDNF pathway in mice
This study was to investigate the effect of AV treatment on depressive behaviors. Herein, we show that AV treatment had antidepressant-like effect in physiological conditions and antidepressant effect in depressive state which depended on α7 nicotinic acetylcholine receptor (α7nAChR) expression in the ventral hippocampus (vHPC), but not α4β2 nicotinic acetylcholine receptor (α4β2nAchR) expression in vHPC, nor the α7nAChR and α4β2nAchR expression in dorsal hippocampus (dHPC). By using MLA, a selective α7nAChR antagonist, we investigated the role of α7nAChR in AV...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Chao Yang Huan-Yu Ni Jia-Jie Yin Ting Zhou Qiu-Xiang Gu Ting-Ting Chen Cheng-Yun Cai Source Type: research

Icariin inhibits isoproterenol-induced cardiomyocyte hypertropic injury through activating autophagy via the AMPK/mTOR signaling pathway
In conclusion, these findings indicated that ICA attenuated cardiomyocyte hypertrophy induced by ISO and prevented cell injury, and the specific mechanism was mediated by AMPK/mTOR pathway to enhance autophagy and reduce autophagy-related cardiomyocyte apoptosis.PMID:35063771 | DOI:10.1016/j.bbrc.2022.01.029 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Lin Hu Ziyuan Wang Huan Li Jiarui Wei Fengyue Tang Qing Wang Jing Wang Xiaoqiao Zhang Qiufang Zhang Source Type: research

Identification of a novel target site for ATP-independent ERK2 inhibitors
Biochem Biophys Res Commun. 2022 Jan 15;593:73-78. doi: 10.1016/j.bbrc.2022.01.035. Online ahead of print.ABSTRACTExtracellular signal-regulated kinase 2 (ERK2) controls vital physiological processes involving proliferation and differentiation and is a drug target molecule for many diseases such as cancers. In silico screening focusing on an allosteric site that plays a crucial role in substrate anchoring conferred an ERK2 inhibitor (compound 1). However, a competitive binding assay indicated that compound 1 did not bind to the allosteric site. Here, the crystal structure of ERK2 in complex with compound 1 revealed a novel...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Mayu Yoshida Haruna Nagao Hajime Sugiyama Masaaki Sawa Takayoshi Kinoshita Source Type: research

Juggling with fluorescent proteins: Spectrum and structural changes of the mCardinal2 variants
Biochem Biophys Res Commun. 2022 Jan 14;593:79-83. doi: 10.1016/j.bbrc.2022.01.044. Online ahead of print.ABSTRACTmCardinal2 is a red fluorescent protein developed through the designs of mKate, mNeptune and mCardinal. Fluorescence spectrums of mCardinal2 and its five mutants (T143C, T143G, C158A, C158D and M160E) were measured with their quantum yields. C158A and C158D increased brightness with slight changes in fluorescence spectrums while T143C, T143G and M160E decreased brightness with blue shift in fluorescence spectrums, which resulted in green, cyan and green fluorescent proteins respectively. Crystal structures of a...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Tae-Yeon Kim Tae-Sung Yoon Sunghyun Kang Muhammad Afzal Source Type: research

Identification of ERCC8 as a novel cisplatin-resistant gene in esophageal cancer based on genome-scale CRISPR/Cas9 screening
Biochem Biophys Res Commun. 2022 Jan 13;593:84-92. doi: 10.1016/j.bbrc.2022.01.033. Online ahead of print.ABSTRACTEsophageal cancer (ESCA) is one of the most common malignant tumors of the digestive system worldwide. As a first-line drug for chemotherapy, cisplatin resistance is the major obstacle in the successful treatment of esophageal cancer. Previous studies largely failed to identify the key genes associated with cisplatin resistance. Hence, the aim of this study was to screen the cisplatin resistance-related genes of esophageal cancer using CRISPR/Cas9 gene-editing technology and Brunello iBar library. Of note, we i...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Xue Sui Xiaolong Tang Xi Wu Yongshuo Liu Source Type: research

PDGF-BB is involved in phosphate regulation via the phosphate transporters in human neuroblastoma SH-SY5Y cells
Biochem Biophys Res Commun. 2022 Jan 14;593:93-100. doi: 10.1016/j.bbrc.2022.01.045. Online ahead of print.ABSTRACTInorganic phosphate (Pi) is the second most abundant inorganic ion in the body. Since abnormalities in Pi metabolism are risk factors for various diseases, serum Pi levels are strictly controlled. Type-III sodium-dependent Pi transporters, PiT-1 (encoded by SLC20A1) and PiT-2 (encoded by SLC20A2), are distributed throughout the tissues of the body, including the central nervous system, and are known to be responsible for extracellular to intracellular Pi transport. Platelet-derived growth factor (PDGF) is a ma...
Source: Biochemical and Biophysical Research communications - January 22, 2022 Category: Biochemistry Authors: Naoko Takase Masatoshi Inden Yuto Murayama Ayane Mishima Hisaka Kurita Isao Hozumi Source Type: research

Binding mode of brazzein to the taste receptor based on crystal structure and docking simulation
Biochem Biophys Res Commun. 2022 Jan 7;592:119-124. doi: 10.1016/j.bbrc.2022.01.004. Online ahead of print.ABSTRACTSeveral natural substances including protein produce sweet taste. Brazzein, derived from the plant Pentadipladra brazzeana, is one of the sweet proteins that bind to the taste receptor with stronger sweetness than sugar. Mutations of this protein affect its flavour, yielding higher sweetness in D29K and lower sweetness in R43A. To elucidate its sweet mechanism in the taste receptor, we determined the structures of two variants, D29K and R43A, to a resolution of 1.5 Å and 1.3 Å, respectively. Struct...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Tae-Yeon Kim Eui-Jeon Woo Tae-Sung Yoon Source Type: research

Effects of pulsed electric fields on exciton propagation efficiency along M üller cell intermediate filaments. Possible separation mechanism of high- and low-contrast images by the eye-brain system
Biochem Biophys Res Commun. 2022 Jan 12;593:1-4. doi: 10.1016/j.bbrc.2022.01.021. Online ahead of print.ABSTRACTIn the current study, we tested a possible mechanism of low- and high-contrast image component discrimination by the vertebrate eye-brain system. Apparently the eye-brain system has to discriminate between the low-contrast image component formed by light scattered within the retina, due to interaction of photons with cells and their parts, and the high-contrast image component transmitted by excitons via the quantum mechanism. Presently, effects of pulsed electric fields applied to Müller cell (MC) intermedi...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Igor Khmelinskii Vladimir I Makarov Source Type: research

Neuroprotective effect of exosomes derived from bone marrow mesenchymal stem cells via activating TGR5 and suppressing apoptosis
Biochem Biophys Res Commun. 2022 Jan 13;593:13-19. doi: 10.1016/j.bbrc.2022.01.039. Online ahead of print.ABSTRACTCerebral infarction has become one of the most common neurovascular diseases, and it leads to a high disability and death rate. The exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs-exo) have been viewed as a potential therapeutic method for some diseases. However, the role of BM-MSCs-exo in cerebral infarction remains unclear. Middle cerebral artery occlusion (MCAO) rat and oxygen and glucose deprived cell models were established. Neurological score, animal behaviors, TTC-staining, HE staining,...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Zhiqiang Liu Xing Li Zhongxing Ye Hai Lin Source Type: research

Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways
Biochem Biophys Res Commun. 2022 Jan 7;593:20-27. doi: 10.1016/j.bbrc.2022.01.011. Online ahead of print.ABSTRACTA cancer-inhibiting role of mesenchyme homeobox 2 (MEOX2) has been observed in several malignancies. However, the association between MEOX2 and breast carcinoma has not been addressed. This research focused on investigating the possible relevance of MEOX2 in breast carcinoma. Initial expression analysis by TCGA data uncovered low levels of MEOX2 in breast carcinoma. We then confirmed that MEOX2 was poorly expressed in clinical tumor specimens of breast carcinoma by real-time quantitative PCR and immunoblotting a...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Yun Cai Yi Liu Ye Sun Yu Ren Source Type: research

Periaqueductal gray is required for controlling chronic stress-induced depression-like behavior
CONCLUSIONS: These results demonstrate that psychological stress-elicited depression-like behavior is related to a remarkable decrease in glutamatergic transmission in the vlPAG. The maladaptive behaviors are attributed to hypoactivity of glutamatergic neurons in the vlPAG, and direct enhancement of glutamatergic neuronal activity in the vlPAG rescues depression-like behaviors. The present results prove that vlPAG is critical for controlling stress-induced depression-like behaviors through alteration of glutamatergic transmission.PMID:35051779 | DOI:10.1016/j.bbrc.2022.01.025 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Wei-Hao Peng Hung-Wei Kan Yu-Cheng Ho Source Type: research

Serotype-based evaluation of an optogenetic construct in rat cortical astrocytes
Biochem Biophys Res Commun. 2022 Jan 11;593:35-39. doi: 10.1016/j.bbrc.2022.01.027. Online ahead of print.ABSTRACTOptogenetics is a modern technique which has been recently expanded to non-neuronal cell types, e.g., astrocytes, and involves targeted gene delivery of light-sensitive ion channels like Channelrhodopsin-2 (ChR2). Optogenetic regulation of astrocytic activity can be used for therapeutic intervention of several neurological disorders. Astrocytic gene delivery, viz adeno-associated viral (AAV) vectors, have proven to be robust, time-, and cost-efficient contrary to the generation of transgenic animal models. When...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Lakshmini Balachandar Diana Borrego Jorge Riera Diaz Source Type: research

Spectral signature of multiple sclerosis. Preliminary studies of blood fraction by ATR FTIR technique
Biochem Biophys Res Commun. 2022 Jan 13;593:40-45. doi: 10.1016/j.bbrc.2022.01.046. Online ahead of print.ABSTRACTMultiple sclerosis (MS) is a chronic, neurodegenerative disease of central nervous system, characterized by inflammation, demyelination, and gliosis. It is commonly known the rapid and accurate diagnosis of MS determines treatment success. The standard diagnosis contains clinical symptoms observation, magnetic resonance imaging (MRI) of central nervous system (CNS), and analysis of cerebrospinal fluid (CSF). Nonetheless, since CSF sampling is considered invasive and not all individuals are eligible for MRI we h...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Magdalena Ko łodziej Karolina Chrab ąszcz Ewa Pi ęta Natalia Piergies Julia Rudnicka-Czerwiec Halina Bartosik-Psujek Czes ława Paluszkiewicz Marian Cholewa Wojciech M Kwiatek Source Type: research

Carbonic anhydrase amyloid fibrils composed of laterally associated protofilaments show reduced cytotoxicity
Biochem Biophys Res Commun. 2022 Jan 13;593:46-51. doi: 10.1016/j.bbrc.2022.01.040. Online ahead of print.ABSTRACTCytotoxicity of amyloid fibrils has been shown to depend on their structure. However, specific features of toxic and non-toxic amyloids remain unclear. Here we focus on the relationship between structural characteristics of the fibrils and their cytotoxicity. Bovine carbonic anhydrase B (BCAB) serves as the object of this study because its amyloids reduce cell viability. Limited proteolysis and mass spectrometry were used to determine BCAB regions forming the core of amyloid fibrils. Four BCAB mutants with subs...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Natalya Ryabova Liliia Fakhranurova Vitaly Balobanov Victor Marchenkov Anatoly Glukhov Nelly Ilyina Alexey Kochetov Mariya Suvorina Alexey Surin Natalya Katina Source Type: research

Neuron-derived neurotrophic factor protects against dexamethasone-induced skeletal muscle atrophy
In conclusion, our findings indicated that NDNF prevents skeletal muscle atrophy in vivo and in vitro through reduction of ubiquitin E3-ligases expression, suggesting that NDNF could be a novel therapeutic target of muscle atrophy.PMID:35051783 | DOI:10.1016/j.bbrc.2022.01.028 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Yuta Ozaki Koji Ohashi Naoya Otaka Hayato Ogawa Hiroshi Kawanishi Tomonobu Takikawa Lixin Fang Minako Tatsumi Mikito Takefuji Takashi Enomoto Mohamed Darwish Yoko Iijima Takatoshi Iijima Toyoaki Murohara Noriyuki Ouchi Source Type: research

Binding mode of brazzein to the taste receptor based on crystal structure and docking simulation
Biochem Biophys Res Commun. 2022 Jan 7;592:119-124. doi: 10.1016/j.bbrc.2022.01.004. Online ahead of print.ABSTRACTSeveral natural substances including protein produce sweet taste. Brazzein, derived from the plant Pentadipladra brazzeana, is one of the sweet proteins that bind to the taste receptor with stronger sweetness than sugar. Mutations of this protein affect its flavour, yielding higher sweetness in D29K and lower sweetness in R43A. To elucidate its sweet mechanism in the taste receptor, we determined the structures of two variants, D29K and R43A, to a resolution of 1.5 Å and 1.3 Å, respectively. Struct...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Tae-Yeon Kim Eui-Jeon Woo Tae-Sung Yoon Source Type: research

Effects of pulsed electric fields on exciton propagation efficiency along M üller cell intermediate filaments. Possible separation mechanism of high- and low-contrast images by the eye-brain system
Biochem Biophys Res Commun. 2022 Jan 12;593:1-4. doi: 10.1016/j.bbrc.2022.01.021. Online ahead of print.ABSTRACTIn the current study, we tested a possible mechanism of low- and high-contrast image component discrimination by the vertebrate eye-brain system. Apparently the eye-brain system has to discriminate between the low-contrast image component formed by light scattered within the retina, due to interaction of photons with cells and their parts, and the high-contrast image component transmitted by excitons via the quantum mechanism. Presently, effects of pulsed electric fields applied to Müller cell (MC) intermedi...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Igor Khmelinskii Vladimir I Makarov Source Type: research

Neuroprotective effect of exosomes derived from bone marrow mesenchymal stem cells via activating TGR5 and suppressing apoptosis
Biochem Biophys Res Commun. 2022 Jan 13;593:13-19. doi: 10.1016/j.bbrc.2022.01.039. Online ahead of print.ABSTRACTCerebral infarction has become one of the most common neurovascular diseases, and it leads to a high disability and death rate. The exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs-exo) have been viewed as a potential therapeutic method for some diseases. However, the role of BM-MSCs-exo in cerebral infarction remains unclear. Middle cerebral artery occlusion (MCAO) rat and oxygen and glucose deprived cell models were established. Neurological score, animal behaviors, TTC-staining, HE staining,...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Zhiqiang Liu Xing Li Zhongxing Ye Hai Lin Source Type: research

Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways
Biochem Biophys Res Commun. 2022 Jan 7;593:20-27. doi: 10.1016/j.bbrc.2022.01.011. Online ahead of print.ABSTRACTA cancer-inhibiting role of mesenchyme homeobox 2 (MEOX2) has been observed in several malignancies. However, the association between MEOX2 and breast carcinoma has not been addressed. This research focused on investigating the possible relevance of MEOX2 in breast carcinoma. Initial expression analysis by TCGA data uncovered low levels of MEOX2 in breast carcinoma. We then confirmed that MEOX2 was poorly expressed in clinical tumor specimens of breast carcinoma by real-time quantitative PCR and immunoblotting a...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Yun Cai Yi Liu Ye Sun Yu Ren Source Type: research

Periaqueductal gray is required for controlling chronic stress-induced depression-like behavior
CONCLUSIONS: These results demonstrate that psychological stress-elicited depression-like behavior is related to a remarkable decrease in glutamatergic transmission in the vlPAG. The maladaptive behaviors are attributed to hypoactivity of glutamatergic neurons in the vlPAG, and direct enhancement of glutamatergic neuronal activity in the vlPAG rescues depression-like behaviors. The present results prove that vlPAG is critical for controlling stress-induced depression-like behaviors through alteration of glutamatergic transmission.PMID:35051779 | DOI:10.1016/j.bbrc.2022.01.025 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Wei-Hao Peng Hung-Wei Kan Yu-Cheng Ho Source Type: research

Serotype-based evaluation of an optogenetic construct in rat cortical astrocytes
Biochem Biophys Res Commun. 2022 Jan 11;593:35-39. doi: 10.1016/j.bbrc.2022.01.027. Online ahead of print.ABSTRACTOptogenetics is a modern technique which has been recently expanded to non-neuronal cell types, e.g., astrocytes, and involves targeted gene delivery of light-sensitive ion channels like Channelrhodopsin-2 (ChR2). Optogenetic regulation of astrocytic activity can be used for therapeutic intervention of several neurological disorders. Astrocytic gene delivery, viz adeno-associated viral (AAV) vectors, have proven to be robust, time-, and cost-efficient contrary to the generation of transgenic animal models. When...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Lakshmini Balachandar Diana Borrego Jorge Riera Diaz Source Type: research

Spectral signature of multiple sclerosis. Preliminary studies of blood fraction by ATR FTIR technique
Biochem Biophys Res Commun. 2022 Jan 13;593:40-45. doi: 10.1016/j.bbrc.2022.01.046. Online ahead of print.ABSTRACTMultiple sclerosis (MS) is a chronic, neurodegenerative disease of central nervous system, characterized by inflammation, demyelination, and gliosis. It is commonly known the rapid and accurate diagnosis of MS determines treatment success. The standard diagnosis contains clinical symptoms observation, magnetic resonance imaging (MRI) of central nervous system (CNS), and analysis of cerebrospinal fluid (CSF). Nonetheless, since CSF sampling is considered invasive and not all individuals are eligible for MRI we h...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Magdalena Ko łodziej Karolina Chrab ąszcz Ewa Pi ęta Natalia Piergies Julia Rudnicka-Czerwiec Halina Bartosik-Psujek Czes ława Paluszkiewicz Marian Cholewa Wojciech M Kwiatek Source Type: research

Carbonic anhydrase amyloid fibrils composed of laterally associated protofilaments show reduced cytotoxicity
Biochem Biophys Res Commun. 2022 Jan 13;593:46-51. doi: 10.1016/j.bbrc.2022.01.040. Online ahead of print.ABSTRACTCytotoxicity of amyloid fibrils has been shown to depend on their structure. However, specific features of toxic and non-toxic amyloids remain unclear. Here we focus on the relationship between structural characteristics of the fibrils and their cytotoxicity. Bovine carbonic anhydrase B (BCAB) serves as the object of this study because its amyloids reduce cell viability. Limited proteolysis and mass spectrometry were used to determine BCAB regions forming the core of amyloid fibrils. Four BCAB mutants with subs...
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Natalya Ryabova Liliia Fakhranurova Vitaly Balobanov Victor Marchenkov Anatoly Glukhov Nelly Ilyina Alexey Kochetov Mariya Suvorina Alexey Surin Natalya Katina Source Type: research

Neuron-derived neurotrophic factor protects against dexamethasone-induced skeletal muscle atrophy
In conclusion, our findings indicated that NDNF prevents skeletal muscle atrophy in vivo and in vitro through reduction of ubiquitin E3-ligases expression, suggesting that NDNF could be a novel therapeutic target of muscle atrophy.PMID:35051783 | DOI:10.1016/j.bbrc.2022.01.028 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - January 20, 2022 Category: Biochemistry Authors: Yuta Ozaki Koji Ohashi Naoya Otaka Hayato Ogawa Hiroshi Kawanishi Tomonobu Takikawa Lixin Fang Minako Tatsumi Mikito Takefuji Takashi Enomoto Mohamed Darwish Yoko Iijima Takatoshi Iijima Toyoaki Murohara Noriyuki Ouchi Source Type: research