Experimental and Molecular Medicine This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Mechanism of phase condensation for chromosome architecture and function
This study by Ryu and colleagues explores the patterns behind the molecular mechanisms (processes at a microscopic level) of chromosomal phase separations. The researchers reviewed the role of proteins and DNA molecules in this process. They discovered two distinct working models, Bridging-Induced Phase Separation (BIPS) and Self-association Induced Phase Separation (SIPS), are involved. The study concludes that the mechanism of chromosomal phase separation relies on the dynamics of proteins. This research enhances our understanding of chromosomal functions and could impact future studies in biological sciences.This summar...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Jeongveen Park Jeong-Jun Kim Je-Kyung Ryu Source Type: research

Emerging insights into transcriptional condensates
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01228-9This review delves into the intricate realm of transcriptional regulation, a process that shapes the unique gene expression profiles of different cell types. It focuses on recent advances in elucidating the composition, property and role of transcriptional condensates, which are recently identified liquid-like entities composed of various proteins involved in transcription. Transcriptional condensate is the embodiment of ‘transcription factory’ concept, which contains high concentration of transcriptional proteins and ...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Kwangmin Ryu Gunhee Park Won-Ki Cho Source Type: research

Viral remodeling of the 4D nucleome
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01207-0This research examines how viruses can change the 3D structure of host DNA, focusing on the role of CTCF and cohesin proteins in altering both viral and cellular 3D DNA structures. The scientists used various techniques, including chromosome conformation capture and next-generation sequencing, to study the 3D structure of both viral and cellular genomes. They discovered that viral infections can cause changes in gene loop formation, chromatin accessibility, and shifts in genome compartments. These changes can affect gene a...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Kyoung-Dong Kim Paul M. Lieberman Source Type: research

Advances in the multimodal analysis of the 3D chromatin structure and gene regulation
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01246-7Understanding how genes work and interact within our cells is key to understanding biology and disease. This review looks at the latest methods for analyzing the 3D structure of our genome, focusing on sequencing, and microscopy techniques. It shows how these technologies let us see our DNA structure in 3D and understand how this affects gene activity and cell functions. The authors aim to provide a fuller picture of how DNA’s spatial arrangement affects its function by combining different methods. They discuss methods t...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Man-Hyuk Han Jihyun Park Minhee Park Source Type: research

3C methods in cancer research: recent advances and future prospects
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01236-9The research explores the 3D structure of chromatin—a combination of DNA and proteins in our cells, and its role in cancer. Using Hi-C technology, a method that maps the closeness of different DNA regions within the cell nucleus, researchers have gained insights into these 3D structures, especially in cancer cells. The study involves data analysis using Hi-C to investigate chromatin’s spatial arrangement and its implications for cancer. By studying interactions between various DNA regions, researchers identify crucial ...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Insoo Yoon Uijin Kim Yousuk Song Taesoo Park Dong-Sung Lee Source Type: research

Enhancer–promoter specificity in gene transcription: molecular mechanisms and disease associations
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01233-yThis review summarizes the current understanding of enhancer-promoter interactions (EPIs), a key feature of genome structure, in gene regulation and disease. EPIs are crucial for proper gene activity, and their alteration by mutational events can be pathological, causing, among others, cancer, and neurodevelopmental disorders. The authors provide an overview of various methods used to study 3D genome architecture (the physical structure of chromosomes) and discuss recent insights that these techniques have afforded, especi...
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Meyer J. Friedman Tobias Wagner Haram Lee Michael G. Rosenfeld Soohwan Oh Source Type: research

4D nucleome: dynamic three-dimensional genome organization over time
Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01248-54D nucleome: dynamic three-dimensional genome organization over time (Source: Experimental and Molecular Medicine)
Source: Experimental and Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Hyoung-Pyo Kim Source Type: research

Author Correction: Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure
Experimental & Molecular Medicine, Published online: 24 April 2024; doi:10.1038/s12276-024-01238-7Author Correction: Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure (Source: Experimental and Molecular Medicine)
Source: Experimental and Molecular Medicine - April 24, 2024 Category: Molecular Biology Authors: Youngbum Yoo MyeongHoon Yeon Won-Kyung Kim Hyeon-Bin Shin Seung-Min Lee Mee-Sup Yoon Hyunju Ro Young-Kyo Seo Source Type: research

Loss of SREBP-1c ameliorates iron-induced liver fibrosis by decreasing lipocalin-2
Experimental & Molecular Medicine, Published online: 16 April 2024; doi:10.1038/s12276-024-01213-2Sterol regulatory element-binding protein (SREBP)-1c plays a role in liver fat regulation and is elevated in nonalcoholic steatohepatitis (NASH). Researchers investigated role of SREBP-1c in NASH using mice models and patient samples, focusing on lipocalin-2 (LCN2) regulation. In NASH mice, LCN2 expression was increased, driven by SREBP-1c. LCN2 treatment increased iron accumulation and fibrosis-related gene expression in hepatic stellate cells, dependent on SREBP-1c. LCN2 expression correlated with inflammation and fibros...
Source: Experimental and Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Eun-Ho Lee Jae-Ho Lee Do-Young Kim Young-Seung Lee Yunju Jo Tam Dao Kyung Eun Kim Dae-Kyu Song Ji Hae Seo Young-Kyo Seo Je Kyung Seong Changjong Moon Eugene Han Mi Kyung Kim Seungwan Ryu Minsang Shin Gu Seob Roh Hye Ra Jung Timothy F. Osborne Dongryeol Ry Source Type: research

Overcoming BRAF and CDK4/6 inhibitor resistance by inhibiting MAP3K3-dependent protection against YAP lysosomal degradation
Experimental & Molecular Medicine, Published online: 16 April 2024; doi:10.1038/s12276-024-01210-5Cancer treatment often uses specific drugs, but sometimes the body can resist these, causing the cancer to return. A study led by Yonsei University College of Medicine researchers looked at the role of a protein, yes-associated protein (YAP), in this drug resistance. They found that another protein, MAP3K3, helps keep YAP stable, which then helps the body resist cancer drugs. By stopping MAP3K3, the researchers could lower YAP levels and overcome drug resistance in breast cancer and skin cancer cells. This suggests that fo...
Source: Experimental and Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Sanghyun Park Won-Ji Ryu Tae Yeong Kim Yumi Hwang Hyun Ju Han Jeong Dong Lee Gun Min Kim Joohyuk Sohn Sang Kyum Kim Min Hwan Kim Joon Kim Source Type: research

Central neurocytoma exhibits radial glial cell signatures with FGFR3 hypomethylation and overexpression
This study used advanced technology to analyze the genetic makeup of CN. The researchers found that CNs don't have any major repeated changes in their genes, gene combinations, or gene copies that could cause the tumor. Instead, they found that the increase in activity of the PI3K-AKT pathway (a pathway involved in cell growth) and changes in several pathways involved in nerve cell development are controlled by an increase in FGFR3 (a protein that helps cells grow and divide) in CNs. This research provides important information about the genetics of CN and could help guide future research and treatments.This summary was in...
Source: Experimental and Molecular Medicine - April 12, 2024 Category: Molecular Biology Authors: Yeajina Lee Tamrin Chowdhury Sojin Kim Hyeon Jong Yu Kyung-Min Kim Ho Kang Min-Sung Kim Jin Wook Kim Yong-Hwy Kim So Young Ji Kihwan Hwang Jung Ho Han Jinha Hwang Seong-Keun Yoo Kyu Sang Lee Gheeyoung Choe Jae-Kyung Won Sung-Hye Park Yong Kyu Lee Joo Heon Source Type: research

Regulation of cargo selection in exosome biogenesis and its biomedical applications in cancer
Experimental & Molecular Medicine, Published online: 05 April 2024; doi:10.1038/s12276-024-01209-yExtracellular vesicles (EVs)—tiny structures produced by cells that are important for cell-to-cell communication—contain various molecules, a process known as cargo sorting. However, how these molecules are selected and packaged into EVs is not fully understood. This research by Lee, Shin, and Chae provides a detailed analysis of our current knowledge of cargo sorting in exosome biogenesis. They reviewed the literature on the types of molecules in exosome cargo, the factors and machinery controlling cargo selection and...
Source: Experimental and Molecular Medicine - April 5, 2024 Category: Molecular Biology Authors: Yu Jin Lee Kyeong Jin Shin Young Chan Chae Source Type: research

Entosis: the core mechanism and crosstalk with other cell death programs
In this study, the authors review recent findings uncovering the molecular regulation of entosis and dicuss its potential implications in cancer. They discuss how entosis is controlled by a network of regulations involving cell adhesion, the RhoA–ROCK signaling pathway, and actomyosin contraction. Interestingly, entosis can occur alongside other cell death forms in populations, suggesting it may influence how cell groups respond to stress. The study concludes that entosis may contribute to aggressive cancers’ development by favoring cells with low tension setpoints, promoting the creation of aneuploid cell lineages, an...
Source: Experimental and Molecular Medicine - April 2, 2024 Category: Molecular Biology Authors: Sunghoon Kim Donghyuk Lee Sung Eun Kim Michael Overholtzer Source Type: research

Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure
Experimental & Molecular Medicine, Published online: 01 April 2024; doi:10.1038/s12276-024-01199-xAging often results in a decrease in muscle mass and function, a condition called sarcopenia. This research examines the role of a protein found in mitochondria (the energy factories of cells), cardiolipin synthase 1 (CRLS1), in muscle health and aging. The scientists discovered that levels of CRLS1 and cardiolipin, a fat it produces, decrease in the muscles of older mice. When CRLS1 levels were artificially lowered in young mice, their muscle mass and strength reduced. On the other hand, increasing CRLS1 levels in older m...
Source: Experimental and Molecular Medicine - April 1, 2024 Category: Molecular Biology Authors: Youngbum Yoo MyeongHoon Yeon Won-Kyung Kim Hyeon-Bin Shin Seung-Min Lee Mee-Sup Yoon Hyunju Ro Young-Kyo Seo Source Type: research

Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2
This study could pave the way for new liver disease treatments.This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author. (Source: Experimental and Molecular Medicine)
Source: Experimental and Molecular Medicine - April 1, 2024 Category: Molecular Biology Authors: Wei Dong Qing Li Xing Lu Jianfeng Lan Zhidong Qiu Xuehong Wang Junnan Wang Xiaojiao Zheng Sifan Chen Chong Zhang Junfei Jin Source Type: research