Advances in the multimodal analysis of the 3D chromatin structure and gene regulation

Experimental & Molecular Medicine, Published online: 25 April 2024; doi:10.1038/s12276-024-01246-7Understanding how genes work and interact within our cells is key to understanding biology and disease. This review looks at the latest methods for analyzing the 3D structure of our genome, focusing on sequencing, and microscopy techniques. It shows how these technologies let us see our DNA structure in 3D and understand how this affects gene activity and cell functions. The authors aim to provide a fuller picture of how DNA’s spatial arrangement affects its function by combining different methods. They discuss methods that can concurrently map the landscape of chromatin structure along with the transcriptomic, proteomic, and epigenetic landscape, all critical for biological functions. Such a comprehensive perspective is vital for accurately unraveling the complex interactions and regulatory mechanisms within the nucleus, thereby enhancing our understanding of the functional significance of 3D chromatin architecture.This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
Source: Experimental and Molecular Medicine - Category: Molecular Biology Authors: Source Type: research