Experimental and Molecular Medicine Experimental and Molecular Medicine RSS feedThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

ADAR1-dependent miR-3144-3p editing simultaneously induces MSI2 expression and suppresses SLC38A4 expression in liver cancer
Experimental & Molecular Medicine, Published online: 04 January 2023; doi:10.1038/s12276-022-00916-8Changes made to a small regulatory RNA molecule by RNA-editing enzymes help to promote the growth of liver tumors. Suk Woo Nam from the Catholic University of Korea in Seoul, South Korea, and colleagues showed that in liver cancer cells a microRNA called miR-3144-3p frequently undergoes a common RNA modification that alters its recognition of two target genes, resulting in elevated expression of one and diminished expression of the other. Silencing activity of the former gene or boosting activity of the latter both helpe...
Source: Experimental and Molecular Medicine - January 4, 2023 Category: Molecular Biology Authors: Hyung Seok Kim Min Jeong Na Keun Hong Son Hee Doo Yang Sang Yean Kim Eunbi Shin Jin Woong Ha Soyoung Jeon Keunsoo Kang Kiho Moon Won Sang Park Suk Woo Nam Source Type: research

Stable colonization of Akkermansia muciniphila educates host intestinal microecology and immunity to battle against inflammatory intestinal diseases
Experimental & Molecular Medicine, Published online: 04 January 2023; doi:10.1038/s12276-022-00911-zA modified gut bacterium that can colonize the gut more effectively with the help of magnets could offer a novel treatment for inflammatory bowel diseases (IBD). In experiments on mouse models, Ming Cui at the Chinese Academy of Medical Sciences and Peking Union Medical College, China, and co-workers showed that when the bacterium Akkermansia muciniphila successfully colonizes the gut it repairs damage caused by IBD and boosts the proliferation of another probiotic bacterium, Lactobacillus murinus. To enhance colonizatio...
Source: Experimental and Molecular Medicine - January 4, 2023 Category: Molecular Biology Authors: Bin Wang Xuheng Chen Zhiyuan Chen Huiwen Xiao Jiali Dong Yuan Li Xiaozhou Zeng Jinjian Liu Guoyun Wan Saijun Fan Ming Cui Source Type: research

ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5
Experimental & Molecular Medicine, Published online: 04 January 2023; doi:10.1038/s12276-022-00908-8The identification of a key tumor-suppressing protein could provide biomarkers and treatments for an aggressive and fast-growing form of brain and spinal cord cancer. Glioblastoma multiforme (GBM) is resistant to many forms of treatment, resulting in poor prognosis. By analyzing gene expression databases, Kyung-Chul Choi and Seong Who Kim at the University of Ulsan College of Medicine in Seoul, South Korea, and co-workers found that expression of a regulatory protein, the transcription factor ZBTB7A, is significantly red...
Source: Experimental and Molecular Medicine - January 4, 2023 Category: Molecular Biology Authors: Ji-Hoon Jeong Seung-Ho Park Hyunhee Kim Hae Yun Nam Sung-Hak Kim Minseok Jeong Min-Jeong Kong Jihyun Son Ji-Eun Jeong Ji-Hye Song Seong Who Kim Kyung-Chul Choi Source Type: research

TPX2 prompts mitotic survival via the induction of BCL2L1 through YAP1 protein stabilization in human embryonic stem cells
Experimental & Molecular Medicine, Published online: 04 January 2023; doi:10.1038/s12276-022-00907-9New details of the molecular mechanisms behind problematic genetic aberrations that can affect cultured human embryonic stem cells could help efforts to maintain stable cell lines that hold great promise for treating a wide variety of diseases. Researchers in South Korea led by Hyuk-Jin Cha at Seoul National University investigated abnormalities in stem cell control systems leading to a condition known as survival advantage, which can allow abnormal cells to proliferate. They identified a gene (TPX2) whose expression act...
Source: Experimental and Molecular Medicine - January 4, 2023 Category: Molecular Biology Authors: Yun-Jeong Kim Young-Hyun Go Ho-Chang Jeong Eun-Ji Kwon Seong-Min Kim Hyun Sub Cheong Wantae Kim Hyoung Doo Shin Haeseung Lee Hyuk-Jin Cha Source Type: research

Phenotype-based screening rediscovered benzopyran-embedded microtubule inhibitors as anti-neuroinflammatory agents by modulating the tubulin–p65 interaction
Experimental & Molecular Medicine, Published online: 12 December 2022; doi:10.1038/s12276-022-00903-zA screen for compounds that reduce inflammation in the brain has identified an effective compound with an unexpected mode of action, potentially opening a new therapeutic avenue for neurodegenerative diseases. Neuroinflammation contributes to the pathology of diseases like Alzheimer’s and Parkinson’s, but researchers have struggled to develop drugs targeting such inflammation. South Korean researchers led by Seung Bum Park of Seoul National University and Jongmin Park of Kangwon National University, Chuncheon, scree...
Source: Experimental and Molecular Medicine - December 12, 2022 Category: Molecular Biology Authors: Junhyeong Yim Jaeseok Lee Sihyeong Yi Ja Young Koo Sangmi Oh Hankum Park Seong Soon Kim Myung Ae Bae Jongmin Park Seung Bum Park Source Type: research

Effect of chromatin modifiers on the plasticity and immunogenicity of small-cell lung cancer
Experimental & Molecular Medicine, Published online: 12 December 2022; doi:10.1038/s12276-022-00905-xInvestigating how mutated protein complexes trigger immune evasion during small-cell lung cancer (SCLC) could yield possible therapeutic approaches for this intractable disease. SCLC is driven by loss-of-function mutations in tumour suppressor genes, which in turn alter the activity of chromatin modifiers, protein complexes that alter chromatin, the mixture of DNA and proteins forming chromosomes. Kwon-Sik Park at the University of Virginia in Charlottesville, USA, and co-workers have reviewed current understanding of t...
Source: Experimental and Molecular Medicine - December 12, 2022 Category: Molecular Biology Authors: Nicole A. Kirk Kee-Beom Kim Kwon-Sik Park Source Type: research

Drawing a line between histone demethylase KDM5A and KDM5B: their roles in development and tumorigenesis
Experimental & Molecular Medicine, Published online: 12 December 2022; doi:10.1038/s12276-022-00902-0Two enzymes that catalyze the removal of methyl groups (-CH3) from the histone proteins that bind to and package DNA within chromosomes play subtly different roles in controlling gene activity in normal development and in the onset of some cancers. So Hee Kwon and colleagues at Yonsei University in Incheon, South Korea, review the activities of the closely related ‘demethylase’ proteins known as KDM5A and KDM5B. They appraise mounting evidence supporting involvement of the proteins in maintaining stable healthy deve...
Source: Experimental and Molecular Medicine - December 12, 2022 Category: Molecular Biology Authors: Jung Yoo Go Woon Kim Yu Hyun Jeon Ji Yoon Kim Sang Wu Lee So Hee Kwon Source Type: research

SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
Experimental & Molecular Medicine, Published online: 09 December 2022; doi:10.1038/s12276-022-00891-0In a procedure applicable to many different genes, the celebrated gene-editing technology known as CRISPR can insert a drug-controlled intronic ‘off switch’ into selected genes in mice via one-step zygote injections, allowing fine-tuned investigation of gene function. A research team led by Szu-Hsien Sam Wu and Bon-Kyoung Koo developed the procedure to generate intron-based conditional knock-out alleles. Here, the genetic modification is achieved using CRISPR gene-editing to insert a Short Conditional intrON called ...
Source: Experimental and Molecular Medicine - December 9, 2022 Category: Molecular Biology Authors: Szu-Hsien Sam Wu Heetak Lee R éka Szép-Bakonyi Gabriele Colozza Ayse Boese Krista R. Gert Natalia Hallay Ji-Hyun Lee Jihoon Kim Yi Zhu Margot M. Linssen Sandra Pilat-Carotta Peter Hohenstein Hans-Christian Theussl Andrea Pauli Bon-Kyoung Koo Source Type: research

Loss of KDM5B ameliorates pathological cardiac fibrosis and dysfunction by epigenetically enhancing ATF3 expression
Experimental & Molecular Medicine, Published online: 08 December 2022; doi:10.1038/s12276-022-00904-yAn enzyme that is increased in heart tissues during cardiac fibrosis could provide a valuable treatment target. Injury or excessive pressure in the heart during disease can trigger cardiac remodeling: changes in the heart’s shape, size and function that often result in heart failure. A key feature of this remodeling is cardiac fibrosis, where heart cells are overactivated triggering excessive collagen deposition. Targeted therapies are limited. A team led by Zhenzhen Zhan at Tongji University and Xingguang Liu at Nava...
Source: Experimental and Molecular Medicine - December 8, 2022 Category: Molecular Biology Authors: Bo Wang Yong Tan Yunkai Zhang Sheng Zhang Xuewen Duan Yuyu Jiang Tong Li Qingqing Zhou Xingguang Liu Zhenzhen Zhan Source Type: research

ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis
Experimental & Molecular Medicine, Published online: 06 December 2022; doi:10.1038/s12276-022-00901-1Cells that line airway sacs in the lungs react to the mechanical strain imposed by ventilators by activating a stress response pathway that may be targetable to prevent or treat ventilator-induced pulmonary fibrosis. A team from Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine in China led by Zhengyu He and Yuan Gao profiled gene expression patterns in mice that developed lung fibrosis following mechanical ventilation and in cultured airway sac cells exposed to deforming tension in the laboratory. The r...
Source: Experimental and Molecular Medicine - December 6, 2022 Category: Molecular Biology Authors: Ri Tang Shuya Mei Qiaoyi Xu Jinhua Feng Yang Zhou Shunpeng Xing Zhengyu He Yuan Gao Source Type: research

Mitochondrial PARP1 regulates NAD+-dependent poly ADP-ribosylation of mitochondrial nucleoids
Experimental & Molecular Medicine, Published online: 06 December 2022; doi:10.1038/s12276-022-00894-xAn enzyme critical to healthy DNA repair may help scientists clarify the mechanisms behind mitochondrial dysfunction in aging. As humans age, DNA damage increases, triggering increased activity of the poly [ADP-ribose] polymerase 1 enzyme (PARP1). However, persistent PARP1 activation may be detrimental and could even accelerate aging. PARP1 activity in cell nuclei is well documented, but whether the enzyme is present in mitochondria is disputed. Vilhelm Bohr at the National Institute of Aging, Baltimore, USA, and co-wor...
Source: Experimental and Molecular Medicine - December 6, 2022 Category: Molecular Biology Authors: Jong-Hyuk Lee Mansoor Hussain Edward W. Kim Shang-Jung Cheng Anthony K. L. Leung Nima Borhan Fakouri Deborah L. Croteau Vilhelm A. Bohr Source Type: research

Inflammation promotes synucleinopathy propagation
Experimental & Molecular Medicine, Published online: 06 December 2022; doi:10.1038/s12276-022-00895-wThe growth of protein aggregates that cause neurodegenerative diseases is greatly accelerated by inflammatory immune responses, researchers in Korea and Germany have shown. Diseases such as Alzheimer’s and Parkinson’s show unique patterns of aggregates, which spread via the cell-to-cell transfer of proteins such as α-synuclein. To investigate other factors that may contribute to aggregation, Seung-Jae Lee at Seoul National University, South Korea, and co-workers developed a variant of α-synuclein called V40G, whic...
Source: Experimental and Molecular Medicine - December 6, 2022 Category: Molecular Biology Authors: Tae-Kyung Kim Eun-Jin Bae Byung Chul Jung Minsun Choi Soo Jean Shin Sung Jun Park Jeong Tae Kim Min Kyo Jung Ayse Ulusoy Mi-Young Song Jun Sung Lee He-Jin Lee Donato A. Di Monte Seung-Jae Lee Source Type: research

Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing
Experimental & Molecular Medicine, Published online: 02 December 2022; doi:10.1038/s12276-022-00892-zMethods that analyze single-cell RNA sequences in fresh clinical samples are skewed toward examining a different subset of cell types than methods analyzing single-nucleus sequences in frozen tissues. A team led by Chang Eun Yoo and Woong-Yang Park from the Samsung Medical Center in Seoul, South Korea, applied two different RNA-sequencing techniques in parallel on a common set of colon and liver tissues collected from cancer patients. They showed that single-cell profiling, which works best with fresh samples that can b...
Source: Experimental and Molecular Medicine - December 2, 2022 Category: Molecular Biology Authors: Jin-Mi Oh Minae An Dae-Soon Son Jinhyuk Choi Yong Beom Cho Chang Eun Yoo Woong-Yang Park Source Type: research

Neurovascular coupling in bone regeneration
Experimental & Molecular Medicine, Published online: 29 November 2022; doi:10.1038/s12276-022-00899-6Further exploration of the interactions between nerve cells and vascularization (blood vessel development into tissue) during bone repair could provide therapeutic targets for bone diseases, including osteoarthritis and bone cancer. Seungyoung Lee at Incheon National University (Incheon, South Korea), Aaron James at Johns Hopkins School of Medicine (Baltimore, USA), and co-workers reviewed the roles of peripheral nerve cell groups and blood vessels in the regulation of bone remodeling. The growth of peripheral nerves an...
Source: Experimental and Molecular Medicine - November 29, 2022 Category: Molecular Biology Authors: Qizhi Qin Seungyong Lee Nirali Patel Kalah Walden Mario Gomez-Salazar Benjamin Levi Aaron W. James Source Type: research

Dissecting exosome inhibitors: therapeutic insights into small-molecule chemicals against cancer
Experimental & Molecular Medicine, Published online: 29 November 2022; doi:10.1038/s12276-022-00898-7Understanding how cells produce and release exosomes, nano-sized membrane-bound vesicles containing biological molecules, holds promise for developing new cancer therapies. Tumors release exosomes that remodel the nearby environment to promote cancer growth, suppress the immune response and induce drug resistance. Although some aspects of exosomes have been studied, little attention has been paid to exosome inhibitors and how they work. Moon-Chang Baek at Kyungpook National University in Daegu, South Korea, and co-worke...
Source: Experimental and Molecular Medicine - November 29, 2022 Category: Molecular Biology Authors: Jong Hyun Kim Chan-Hyeong Lee Moon-Chang Baek Source Type: research