The efficient synthesis and purification of 2 ′3’- cGAMP from Escherichia coli
Agonists of the stimulator of interferon genes (STING) pathway are being explored as potential immunotherapeutics for the treatment of cancer and as vaccine adjuvants for infectious diseases. Although chemical synthesis of 2′3’ - cyclic Guanosine Monophosphate–Adenosine Monophosphate (cGAMP) is commercially feasible, the process results in low yields and utilizes organic solvents. To pursue an efficient and environmentally friendly process for the production of cGAMP, we focused on the recombinant production of cGAMP via a whole-cell biocatalysis platform utilizing the murine cyclic Guanosine monophosphate–Adenosin...
Source: Frontiers in Microbiology - March 8, 2024 Category: Microbiology Source Type: research
STING dependent BAX-IRF3 signaling results in apoptosis during late-stage Coxiella burnetii infection
Cell Death & Disease, Published online: 08 March 2024; doi:10.1038/s41419-024-06573-1STING dependent BAX-IRF3 signaling results in apoptosis during late-stage Coxiella burnetii infection (Source: Cell death and disease)
Source: Cell death and disease - March 8, 2024 Category: Internal Medicine Authors: Manish Chauhan Chelsea A. Osbron Heather S. Koehler Alan G. Goodman Source Type: research
ASFV pA151R negatively regulates type I IFN production via degrading E3 ligase TRAF6
In this study, we demonstrated that ASFV pA151R negatively regulated type I IFN production. Ectopic expression of pA151R dramatically inhibited K63-linked polyubiquitination and Ser172 phosphorylation of TANK-binding kinase 1 (TBK1). Mechanically, we demonstrated that E3 ligase TNF receptor-associated factor 6 (TRAF6) participated in the ubiquitination of TBK1 in cGAS-STING signaling pathway. We showed that pA151R interacted with TRAF6 and degraded it through apoptosis pathway, leading to the disruption of TBK1 and TRAF6 interaction. Moreover, we clarified that the amino acids H102, C109, C132, and C135 in pA151R were cruc...
Source: Herpes - March 7, 2024 Category: Infectious Diseases Authors: You Li Li Huang Hui Li Yingqi Zhu Zilong Yu Xiaojie Zheng Changjiang Weng Wen-Hai Feng Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
ASFV pA151R negatively regulates type I IFN production via degrading E3 ligase TRAF6
In this study, we demonstrated that ASFV pA151R negatively regulated type I IFN production. Ectopic expression of pA151R dramatically inhibited K63-linked polyubiquitination and Ser172 phosphorylation of TANK-binding kinase 1 (TBK1). Mechanically, we demonstrated that E3 ligase TNF receptor-associated factor 6 (TRAF6) participated in the ubiquitination of TBK1 in cGAS-STING signaling pathway. We showed that pA151R interacted with TRAF6 and degraded it through apoptosis pathway, leading to the disruption of TBK1 and TRAF6 interaction. Moreover, we clarified that the amino acids H102, C109, C132, and C135 in pA151R were cruc...
Source: Herpes - March 7, 2024 Category: Infectious Diseases Authors: You Li Li Huang Hui Li Yingqi Zhu Zilong Yu Xiaojie Zheng Changjiang Weng Wen-Hai Feng Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
Role of mitochondrial dysfunction in kidney disease: Insights from the cGAS-STING signaling pathway
Chin Med J (Engl). 2024 Mar 6. doi: 10.1097/CM9.0000000000003022. Online ahead of print.ABSTRACTOver the past decade, mitochondrial dysfunction has been investigated as a key contributor to acute and chronic kidney disease. However, the precise molecular mechanisms linking mitochondrial damage to kidney disease remain elusive. The recent insights into the cyclic guanosine monophosphate-adenosine monophosphate (GMP-AMP) synthetase (cGAS)-stimulator of interferon gene (STING) signaling pathway have revealed its involvement in many renal diseases. One of these findings is that mitochondrial DNA (mtDNA) induces inflammatory re...
Source: Chinese Medical Journal - March 6, 2024 Category: General Medicine Authors: Lu Li Fei Liu Chunyue Feng Zhenjie Chen Nan Zhang Jianhua Mao Source Type: research
BRD4 promotes LPS-induced endothelial cells senescence via activating and cooperating STING-IRF3 pathway
Cell Signal. 2024 Mar 4:111127. doi: 10.1016/j.cellsig.2024.111127. Online ahead of print.ABSTRACTEndothelial cells (ECs) senescence is closely associated with the initiation and development of multiple age-related cardiovascular diseases. It is necessary to explore the underlying molecular mechanisms of ECs senescence, which is not only the basis to decipher cellular senescence, but also a novel therapeutic target for the endothelial senescence-related diseases. BRD4, a key epigenetic regulator, is universally related to gene expression regulation and has been reported to accelerate cell senescence. Besides, emerging evid...
Source: Cellular Signalling - March 6, 2024 Category: Cytology Authors: Ruigong Zhu Lei Zhang Hao Zhang Zhifeng Hu Source Type: research
Role of mitochondrial dysfunction in kidney disease: Insights from the cGAS-STING signaling pathway
Chin Med J (Engl). 2024 Mar 6. doi: 10.1097/CM9.0000000000003022. Online ahead of print.ABSTRACTOver the past decade, mitochondrial dysfunction has been investigated as a key contributor to acute and chronic kidney disease. However, the precise molecular mechanisms linking mitochondrial damage to kidney disease remain elusive. The recent insights into the cyclic guanosine monophosphate-adenosine monophosphate (GMP-AMP) synthetase (cGAS)-stimulator of interferon gene (STING) signaling pathway have revealed its involvement in many renal diseases. One of these findings is that mitochondrial DNA (mtDNA) induces inflammatory re...
Source: Chinese Medical Journal - March 6, 2024 Category: General Medicine Authors: Lu Li Fei Liu Chunyue Feng Zhenjie Chen Nan Zhang Jianhua Mao Source Type: research
BRD4 promotes LPS-induced endothelial cells senescence via activating and cooperating STING-IRF3 pathway
Cell Signal. 2024 Mar 4:111127. doi: 10.1016/j.cellsig.2024.111127. Online ahead of print.ABSTRACTEndothelial cells (ECs) senescence is closely associated with the initiation and development of multiple age-related cardiovascular diseases. It is necessary to explore the underlying molecular mechanisms of ECs senescence, which is not only the basis to decipher cellular senescence, but also a novel therapeutic target for the endothelial senescence-related diseases. BRD4, a key epigenetic regulator, is universally related to gene expression regulation and has been reported to accelerate cell senescence. Besides, emerging evid...
Source: Cellular Signalling - March 6, 2024 Category: Cytology Authors: Ruigong Zhu Lei Zhang Hao Zhang Zhifeng Hu Source Type: research
Role of mitochondrial dysfunction in kidney disease: Insights from the cGAS-STING signaling pathway
Chin Med J (Engl). 2024 Mar 6. doi: 10.1097/CM9.0000000000003022. Online ahead of print.ABSTRACTOver the past decade, mitochondrial dysfunction has been investigated as a key contributor to acute and chronic kidney disease. However, the precise molecular mechanisms linking mitochondrial damage to kidney disease remain elusive. The recent insights into the cyclic guanosine monophosphate-adenosine monophosphate (GMP-AMP) synthetase (cGAS)-stimulator of interferon gene (STING) signaling pathway have revealed its involvement in many renal diseases. One of these findings is that mitochondrial DNA (mtDNA) induces inflammatory re...
Source: Chinese Medical Journal - March 6, 2024 Category: General Medicine Authors: Lu Li Fei Liu Chunyue Feng Zhenjie Chen Nan Zhang Jianhua Mao Source Type: research
Nanoplastics causes heart aging/myocardial cell senescence through the Ca2+/mtDNA/cGAS-STING signaling cascade
Nanoplastics (NPs) are now a new class of pollutants widely present in the soil, atmosphere, freshwater and marine environments. Nanoplastics can rapidly penetrate cell membranes and accumulate in human tissue... (Source: Journal of Nanobiotechnology)
Source: Journal of Nanobiotechnology - March 6, 2024 Category: Nanotechnology Authors: Kaihao Wang, Yipeng Du, Peixin Li, Chang Guan, Min Zhou, Lanlan Wu, Zengfu Liu and Zheng Huang Tags: Research Source Type: research
Smart responsive Fe/Mn nanovaccine triggers liver cancer immunotherapy via pyroptosis and pyroptosis-boosted cGAS-STING activation
The prognosis for hepatocellular carcinoma (HCC) remains suboptimal, characterized by high recurrence and metastasis rates. Although metalloimmunotherapy has shown potential in combat... (Source: Journal of Nanobiotechnology)
Source: Journal of Nanobiotechnology - March 6, 2024 Category: Nanotechnology Authors: Qianying Du, Ying Luo, Lian Xu, Chier Du, Wenli Zhang, Jie Xu, Yun Liu, Bo Liu, Sijin Chen, Yi Wang, Zhigang Wang, Haitao Ran, Junrui Wang and Dajing Guo Tags: Research Source Type: research
Radiofrequency hyperthermia enhances the effect of OK-432 for Hepatocellular carcinoma by activating of TLR4-cGAS-STING pathway
In conclusion, RFH in combination with OK-432 might reduce the residual and recurrent tumor after RFA of large and irregular HCCs, and serve as a new option for other solid malignancies treated by RFA.PMID:38442584 | DOI:10.1016/j.intimp.2024.111769 (Source: International Immunopharmacology)
Source: International Immunopharmacology - March 5, 2024 Category: Allergy & Immunology Authors: Bo Sun Qingqing Zhang Tao Sun Jiayun Liu Yanyan Cao Bin Liang Chuansheng Zheng Xuefeng Kan Source Type: research
