Baohuoside I Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Apoptosis Signaling and NF-kB Pathway
This study aimed to investigate the inhibitory effect of Baohuoside I on the Human Hepatocellular Carcinoma (HCC) cell lines QGY7703, and underlying mechanisms. QGY7703 cells were used as the model to assess the function of Baohuoside I in vitro. The effects of Baohuoside I on QGY7703 cells' growth, proliferation, and invasiveness were confirmed by CCK-8, lactate dehydrogenase release, and invasion assays. Cell apoptosis was analyzed by flow cytometry, and the levels of cleaved Caspase-3, Bax, and Bcl-2 were quantified by western blot. Western blot analysis, nuclear translocation of NF-κB, and Q-PCR were used to measure t...
Source: Chemistry and Biodiversity - April 27, 2021 Category: Biochemistry Authors: Yunlong Sun Bo Pang Yingzhe Wang Jinglei Xiao Dacheng Jiang Source Type: research
Baohuoside I Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Apoptosis Signaling and NF-kB Pathway
This study aimed to investigate the inhibitory effect of Baohuoside I on the Human Hepatocellular Carcinoma (HCC) cell lines QGY7703, and underlying mechanisms. QGY7703 cells were used as the model to assess the function of Baohuoside I in vitro. The effects of Baohuoside I on QGY7703 cells' growth, proliferation, and invasiveness were confirmed by CCK-8, lactate dehydrogenase release, and invasion assays. Cell apoptosis was analyzed by flow cytometry, and the levels of cleaved Caspase-3, Bax, and Bcl-2 were quantified by western blot. Western blot analysis, nuclear translocation of NF-κB, and Q-PCR were used to measure t...
Source: Chemistry and Biodiversity - April 27, 2021 Category: Biochemistry Authors: Yunlong Sun Bo Pang Yingzhe Wang Jinglei Xiao Dacheng Jiang Source Type: research
High-level expression of a novel multifunctional GH3 family β-xylosidase/α-arabinosidase/β-glucosidase from Dictyoglomus turgidum in Escherichia coli
Bioorg Chem. 2021 Apr 20;111:104906. doi: 10.1016/j.bioorg.2021.104906. Online ahead of print.ABSTRACTA novel β-xylosidase Dt-2286 from Dictyoglomus turgidum was cloned and overexpressed in Escherichia coli BL21 (DE3). Dt-2286 belonging to glycoside hydrolase (GH) family 3 encodes a polypeptide with 762 amino acid residues with a molecular weight of 85.1 kDa. By optimization of the growth and induction conditions, the activity of β-xylosidase reached 273 U/mL, which is the highest yield reported to date from E. coli in a shake-flask. The optimal activities of the purified Dt-2286 were found at pH 5.0 and 98 °C. It also ...
Source: Bioorganic Chemistry - April 24, 2021 Category: Chemistry Authors: Xinyi Tong Zhipeng Qi Daiyi Zheng Jianjun Pei Qi Li Linguo Zhao Source Type: research
High-level expression of a novel multifunctional GH3 family β-xylosidase/α-arabinosidase/β-glucosidase from Dictyoglomus turgidum in Escherichia coli
Bioorg Chem. 2021 Apr 20;111:104906. doi: 10.1016/j.bioorg.2021.104906. Online ahead of print.ABSTRACTA novel β-xylosidase Dt-2286 from Dictyoglomus turgidum was cloned and overexpressed in Escherichia coli BL21 (DE3). Dt-2286 belonging to glycoside hydrolase (GH) family 3 encodes a polypeptide with 762 amino acid residues with a molecular weight of 85.1 kDa. By optimization of the growth and induction conditions, the activity of β-xylosidase reached 273 U/mL, which is the highest yield reported to date from E. coli in a shake-flask. The optimal activities of the purified Dt-2286 were found at pH 5.0 and 98 °C. It also ...
Source: Bioorganic Chemistry - April 24, 2021 Category: Chemistry Authors: Xinyi Tong Zhipeng Qi Daiyi Zheng Jianjun Pei Qi Li Linguo Zhao Source Type: research
IJERPH, Vol. 18, Pages 3671: A Placebo-Controlled, Pseudo-Randomized, Crossover Trial of Botanical Agents for Gulf War Illness: Reishi Mushroom (Ganoderma lucidum), Stinging Nettle (Urtica dioica), and Epimedium (Epimedium sagittatum)
This report is third in a three-part clinical trial series screening potential treatments for Gulf War Illness (GWI). The goal of the project was to rapidly identify agents to prioritize for further efficacy research. We used a placebo-controlled, pseudo-randomized, crossover design to test the effects of reishi mushroom (Ganoderma lucidum), stinging nettle (Uritca dioica), and epimedium (Epimedium sagittatum) in 29 men with GWI. Participants completed 30 days of symptom reports for baseline, then a botanical line consisting of 30 days of placebo, followed by 30 days each of lower-dose and higher-dose botanical. After comp...
Source: International Journal of Environmental Research and Public Health - April 1, 2021 Category: Environmental Health Authors: Jarred Younger Emily K. Donovan Kathleen S. Hodgin Timothy J. Ness Tags: Article Source Type: research
Corrigendum to “A novel herbal formulation consisting of red ginseng extract and Epimedium koreanum Nakai-attenuated dextran sulfate sodium induced colitis in mice” [J Ginseng Res 44 (2020) 833–842]
Publication date: March 2021Source: Journal of Ginseng Research, Volume 45, Issue 2Author(s): Evelyn Saba, Yuan Yee Lee, Minki Kim, Sun-Hee Hyun, Chae-Kyu Park, Eunjung Son, Dong-Seon Kim, Sung-Dae Kim, Man Hee Rhee (Source: Journal of Ginseng Research)
Source: Journal of Ginseng Research - March 24, 2021 Category: Complementary Medicine Source Type: research
Molecules, Vol. 26, Pages 1520: Direct Flavonoid-Focused Chemical Comparison Among Three Epimedium Plants by Online Liquid Extraction –High Performance Liquid Chromatography–Tandem Mass Spectrometry
Molecules, Vol. 26, Pages 1520: Direct Flavonoid-Focused Chemical Comparison Among Three Epimedium Plants by Online Liquid Extraction–High Performance Liquid Chromatography–Tandem Mass Spectrometry
Molecules doi: 10.3390/molecules26061520
Authors:
Xia Xu
Ting Li
Ke Zhang
Yan Cao
Li Liu
Shilin Zhang
Pengfei Tu
Yuelin Song
Yunfang Zhao
Jun Li
It is usually a tedious task to profile the chemical composition of a given herbal medicine (HM) using high performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) due to the time-consuming sample preparation and laborious post-acquisition...
Source: Molecules - March 10, 2021 Category: Chemistry Authors: Xia Xu Ting Li Ke Zhang Yan Cao Li Liu Shilin Zhang Pengfei Tu Yuelin Song Yunfang Zhao Jun Li Tags: Article Source Type: research
The Effect of TCM-Induced HAMP on Key Enzymes in the Hydrolysis of AD Model Cells
Neurochem Res. 2021 Mar 8. doi: 10.1007/s11064-021-03235-y. Online ahead of print.ABSTRACTAlzheimer's disease (AD) process is characterized classically by two hallmark pathologies: β-amyloid (Aβ) plaque deposition and neurofibrillary tangles of hyperphosphorylated tau. Aβ peptides play an important role in AD, but despite much effort the molecular mechanisms of how Aβ contributes to AD remain unclear. The present study evaluated the effects of the active components of Epimedium, Astragalus and Radix Puerariae induced HAMP on key enzymes in the hydrolysis of APP in HT22 cells. The active components of Epimedium, Astraga...
Source: Neurochemical Research - March 8, 2021 Category: Neuroscience Authors: Dong Xian-Hui Ma Dong-Xue Zhang Tian-Ci He Xiao-Ping Xu Li-Jun Liu Ya-Lei Li Hao Gao Wei-Juan Source Type: research
The Effect of TCM-Induced HAMP on Key Enzymes in the Hydrolysis of AD Model Cells
Neurochem Res. 2021 Mar 8. doi: 10.1007/s11064-021-03235-y. Online ahead of print.ABSTRACTAlzheimer's disease (AD) process is characterized classically by two hallmark pathologies: β-amyloid (Aβ) plaque deposition and neurofibrillary tangles of hyperphosphorylated tau. Aβ peptides play an important role in AD, but despite much effort the molecular mechanisms of how Aβ contributes to AD remain unclear. The present study evaluated the effects of the active components of Epimedium, Astragalus and Radix Puerariae induced HAMP on key enzymes in the hydrolysis of APP in HT22 cells. The active components of Epimedium, Astraga...
Source: Neurochemical Research - March 8, 2021 Category: Neuroscience Authors: Dong Xian-Hui Ma Dong-Xue Zhang Tian-Ci He Xiao-Ping Xu Li-Jun Liu Ya-Lei Li Hao Gao Wei-Juan Source Type: research
The Effect of TCM-Induced HAMP on Key Enzymes in the Hydrolysis of AD Model Cells
Neurochem Res. 2021 Mar 8. doi: 10.1007/s11064-021-03235-y. Online ahead of print.ABSTRACTAlzheimer's disease (AD) process is characterized classically by two hallmark pathologies: β-amyloid (Aβ) plaque deposition and neurofibrillary tangles of hyperphosphorylated tau. Aβ peptides play an important role in AD, but despite much effort the molecular mechanisms of how Aβ contributes to AD remain unclear. The present study evaluated the effects of the active components of Epimedium, Astragalus and Radix Puerariae induced HAMP on key enzymes in the hydrolysis of APP in HT22 cells. The active components of Epimedium, Astraga...
Source: Neurochemical Research - March 8, 2021 Category: Neuroscience Authors: Dong Xian-Hui Ma Dong-Xue Zhang Tian-Ci He Xiao-Ping Xu Li-Jun Liu Ya-Lei Li Hao Gao Wei-Juan Source Type: research
Corrigendum for “A novel herbal formulation consisting of red ginseng extract and Epimedium koreanum Nakai-attenuated dextran sulfate sodium induced colitis in mice” [J Ginseng Res 44 (2020) 833–842]
Publication date: Available online 6 March 2021Source: Journal of Ginseng ResearchAuthor(s): Evelyn Saba, Yuan Yee Lee, Minki Kim, Sun-Hee Hyun, Chae-Kyu Park, Eunjung Son, Dong-Seon Kim, Sung-Dae Kim, Man Hee Rhee (Source: Journal of Ginseng Research)
Source: Journal of Ginseng Research - March 7, 2021 Category: Complementary Medicine Source Type: research
Biotransformation of the total flavonoid extract of epimedium into icaritin by two thermostable glycosidases from Dictyoglomus thermophilum DSM3960
Publication date: Available online 4 March 2021Source: Process BiochemistryAuthor(s): Shanshan Zhang, Jianianhua Luo, Yurong Dong, Zhenzhong Wang, Wei Xiao, Linguo Zhao (Source: Process Biochemistry)
Source: Process Biochemistry - March 5, 2021 Category: Biochemistry Source Type: research
Combined protective effects of icariin and selenomethionine on novel chronic tubulointerstitial nephropathy models < em > in vivo < /em > and < em > vitro < /em >
This study was investigated to evaluate the joint effects of ICA and SeMet on CTIN and explore the mechanism. Based on a novel CTIN model developed in our previous study, mice were randomly divided into five groups (a: control; b: model; c: model+ICA; d: model+SeMet; e: model+ICA+SeMet). Renal tubule epithelial cells were treated with cyclosporine A and ochratoxin A without/with ICA or/and SeMet. The results showed ICA or/and SeMet ameliorated CTIN by inhibiting the uptrends of blood urine nitrogen, serum creatinine, urine protein, urine gravity, histopathological damage degree and collagen Ⅰ deposition. ICA or/and SeMet...
Source: The British Journal of Nutrition - March 5, 2021 Category: Nutrition Authors: Lili Hou Ziman Lin Anyuan Xu Guannan Le Lei Ge Shuiping Liu Azhar Muhmood Fang Gan Kehe Huang Source Type: research
Combined protective effects of icariin and selenomethionine on novel chronic tubulointerstitial nephropathy models < em > in vivo < /em > and < em > vitro < /em >
This study was investigated to evaluate the joint effects of ICA and SeMet on CTIN and explore the mechanism. Based on a novel CTIN model developed in our previous study, mice were randomly divided into five groups (a: control; b: model; c: model+ICA; d: model+SeMet; e: model+ICA+SeMet). Renal tubule epithelial cells were treated with cyclosporine A and ochratoxin A without/with ICA or/and SeMet. The results showed ICA or/and SeMet ameliorated CTIN by inhibiting the uptrends of blood urine nitrogen, serum creatinine, urine protein, urine gravity, histopathological damage degree and collagen Ⅰ deposition. ICA or/and SeMet...
Source: The British Journal of Nutrition - March 5, 2021 Category: Nutrition Authors: Lili Hou Ziman Lin Anyuan Xu Guannan Le Lei Ge Shuiping Liu Azhar Muhmood Fang Gan Kehe Huang Source Type: research
Combined protective effects of icariin and selenomethionine on novel chronic tubulointerstitial nephropathy models < em > in vivo < /em > and < em > vitro < /em >
This study was investigated to evaluate the joint effects of ICA and SeMet on CTIN and explore the mechanism. Based on a novel CTIN model developed in our previous study, mice were randomly divided into five groups (a: control; b: model; c: model+ICA; d: model+SeMet; e: model+ICA+SeMet). Renal tubule epithelial cells were treated with cyclosporine A and ochratoxin A without/with ICA or/and SeMet. The results showed ICA or/and SeMet ameliorated CTIN by inhibiting the uptrends of blood urine nitrogen, serum creatinine, urine protein, urine gravity, histopathological damage degree and collagen Ⅰ deposition. ICA or/and SeMet...
Source: The British Journal of Nutrition - March 5, 2021 Category: Nutrition Authors: Lili Hou Ziman Lin Anyuan Xu Guannan Le Lei Ge Shuiping Liu Azhar Muhmood Fang Gan Kehe Huang Source Type: research
