Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain
J Microbiol Biotechnol. 2022 Feb 11;32(4):1-8. doi: 10.4014/jmb.2112.12036. Online ahead of print.ABSTRACTTo obtain the icaritin with high pharmacological activities from icariin, which content ratio over 58% in the total flavonoids of Epimedium Herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in the medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Zhenghao Wang Chunying Liu Hongshan Yu Bo Wu Baoyu Huai Ziyu Zhuang Changkai Sun Longquan Xu Fengxie Jin Source Type: research
Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway
Toxicol Appl Pharmacol. 2022 Mar 5:115969. doi: 10.1016/j.taap.2022.115969. Online ahead of print.ABSTRACTIcariin (ICA), extracted from Epimedium, is a flavonoid used in traditional Chinese medicine. Di(2-ethylhexyl) phthalate (DEHP) is a phthalate used in commercial products as a plasticizer that can influence the human endocrine and reproduction system. We previously found that ICA reversed DEHP-induced damage through the prevention of reactive oxygen species accumulation and promotion of testosterone secretion. Here we investigated the mechanisms of ICA in promoting testosterone secretion from murine Leydig cells. We us...
Source: Toxicology and Applied Pharmacology - March 8, 2022 Category: Toxicology Authors: Jiandong Sun Weiwei Xu Shuyuan Zheng Chengyu Lv Jianmin Lin Siqi Chen Yonghong Qiu Xia Jiang Eman Draz Shie Wang Source Type: research
Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway
Toxicol Appl Pharmacol. 2022 Mar 5:115969. doi: 10.1016/j.taap.2022.115969. Online ahead of print.ABSTRACTIcariin (ICA), extracted from Epimedium, is a flavonoid used in traditional Chinese medicine. Di(2-ethylhexyl) phthalate (DEHP) is a phthalate used in commercial products as a plasticizer that can influence the human endocrine and reproduction system. We previously found that ICA reversed DEHP-induced damage through the prevention of reactive oxygen species accumulation and promotion of testosterone secretion. Here we investigated the mechanisms of ICA in promoting testosterone secretion from murine Leydig cells. We us...
Source: Toxicology and Applied Pharmacology - March 8, 2022 Category: Toxicology Authors: Jiandong Sun Weiwei Xu Shuyuan Zheng Chengyu Lv Jianmin Lin Siqi Chen Yonghong Qiu Xia Jiang Eman Draz Shie Wang Source Type: research
Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway
Toxicol Appl Pharmacol. 2022 Mar 5:115969. doi: 10.1016/j.taap.2022.115969. Online ahead of print.ABSTRACTIcariin (ICA), extracted from Epimedium, is a flavonoid used in traditional Chinese medicine. Di(2-ethylhexyl) phthalate (DEHP) is a phthalate used in commercial products as a plasticizer that can influence the human endocrine and reproduction system. We previously found that ICA reversed DEHP-induced damage through the prevention of reactive oxygen species accumulation and promotion of testosterone secretion. Here we investigated the mechanisms of ICA in promoting testosterone secretion from murine Leydig cells. We us...
Source: Toxicology and Applied Pharmacology - March 8, 2022 Category: Toxicology Authors: Jiandong Sun Weiwei Xu Shuyuan Zheng Chengyu Lv Jianmin Lin Siqi Chen Yonghong Qiu Xia Jiang Eman Draz Shie Wang Source Type: research
Trifluoro-icaritin alleviates chronic inflammatory pain through α7nAChR-mediated suppression of HMGB1/NF-κB signaling in the spinal cord of rats
Brain Res Bull. 2022 Feb 21:S0361-9230(22)00053-3. doi: 10.1016/j.brainresbull.2022.02.014. Online ahead of print.ABSTRACTInflammatory pain is a chronic, persistent and serious disease that greatly impacts public health, which is often accompanied by allodynia, hyperalgesia, and spontaneous pain. It is evident that α7 nicotinic acetylcholine receptor (α7nAChR) plays a key role in cholinergic anti-inflammatory pathway and exhibits the inhibition of neuroinflammation in chronic pain. Trifluoro-icaritin (ICTF), a derivative of icaritin from the extract of a genus of Epimedium plant, is identified to possess profound anti-in...
Source: Brain Research - February 24, 2022 Category: Neurology Authors: Yalan Sun Dandan Jia Meng Xue Zhihua Huang Cheng Huang Source Type: research
