Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Identification and Characterization of a Novel Thermostable GDSL-type Lipase from < em > Geobacillus thermocatenulatus < /em >
J Microbiol Biotechnol. 2021 Feb 12;31(3):1-8. doi: 10.4014/jmb.2012.12036. Online ahead of print.ABSTRACTTwo putative genes, lip29 and est29, encoding lipolytic enzymes from the thermophilic bacterium Geobacillus thermocatenulatus KCTC 3921 were cloned and overexpressed in Escherichia coli. The recombinant Lip29 and Est29 were purified 67.3-fold to homogeneity with specific activity of 2.27 U/mg and recovery of 5.8% and 14.4-fold with specific activity of 0.92 U/mg and recovery of 1.3%, respectively. The molecular mass of each purified enzyme was estimated to be 29 kDa by SDS-PAGE. The alignment analysis of amino acid seq...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Eunhye Jo Jihye Kim Areum Lee Keumok Moon Jaeho Cha Source Type: research

Construction and Analysis of Food-grade < em > Lactobacillus kefiranofaciens < /em > β-galactosidase Overexpression System
In this study, LacLM and LacZ from L. kefiranofaciens ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n-lacs was constructed from pMG36e-lacs by replacing erythromycin with nisin as selective marker for food-grade expressing systems in Lactobacillus plantarum WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original L. kefiranofaciens. Moreover, the lacto...
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Xi He MingJian Luan Ning Han Ting Wang Xiangzhong Zhao Yanyan Yao Source Type: research

Increased risk of severe gastric symptoms by virulence factors < em > vacAs1c < /em > , < em > alpA < /em > , < em > babA2 < /em > , and < em > hopZ < /em > in < em > Helicobacter pylori < /em > infection
This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms, which will enable to predict the clinical disease outcome due to H. pylori infection.PMID:33622995 | DOI:10.4014/jmb.2101.01023 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 24, 2021 Category: Biotechnology Authors: Dong-Hae Lee Jong-Hun Ha Jeong-Ih Shin Kyu-Min Kim Jeong-Gyu Choi Seorin Park Jin-Sik Park Ji-Hyeun Seo Ji-Shook Park Min-Kyoung Shin Seung-Chul Baik Woo-Kon Lee Hee-Shang Youn Myung-Je Cho Hyung-Lyun Kang Myunghwan Jung Source Type: research

Assessment of Erythrobacter species diversity through pan-genome analysis with newly isolated Erythrobacter sp. 3-20A1M.
In this study, we report the complete genome sequence of the marine bacterium Erythrobacter sp. 3-20A1M. The genome size was 3.1 Mbp and its GC content was 64.8%. In total, 2998 genetic features were annotated, of which 2882 were annotated as functional coding genes. Using the genetic information of Erythrobacter sp. 3-20A1M, we performed pan-genome analysis with other Erythrobacter species. This revealed highly conserved secondary metabolite biosynthesis-related COG functions across Erythrobacter species. Through subsequent secondary metabolite biosynthetic gene cluster prediction and KEGG analysis, the carotenoid biosynt...
Source: Journal of Microbiology and Biotechnology - February 3, 2021 Category: Biotechnology Authors: Cho SH, Jeong Y, Lee E, Ko SR, Ahn CY, Oh HM, Cho BK, Cho S Tags: J Microbiol Biotechnol Source Type: research

Glabridin liposome ameliorating UVB-induced erythema and lethery skin by suppressing inflammatory cytokine production.
This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin ...
Source: Journal of Microbiology and Biotechnology - February 2, 2021 Category: Biotechnology Authors: Zhang C, Lu Y, Ai Y, Xu X, Zhu S, Zhang B, Tang M, Zhang L, He T Tags: J Microbiol Biotechnol Source Type: research

Removal of Salmonella Typhimurium biofilm from food contact surfaces using Quercus infectoria gall extract in combination with a surfactant.
This study suggests nutgall extract + CTAB may be an alternative to commonly used sanitizers to remove biofilm from food contact surfaces in the food industry and household. PMID: 33526753 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 1, 2021 Category: Biotechnology Authors: Damrongsaktrakul P, Ruengvisesh S, Rahothan A, Sukhumrat N, Tuitemwong P, Phung-On I Tags: J Microbiol Biotechnol Source Type: research

Skin commensal fungus Malassezia and its lipases.
Abstract Malassezia is the most abundant genus in the fungal microflora found on human skin, and it is associated with various skin diseases. Among the 18 different species of Malassezia that have been identified to date, M. restricta and M. globosa are the most predominant fungal species found on human skin. Several studies have suggested a possible link between Malassezia and skin disorders. However, our knowledge on the physiology and pathogenesis of Malassezia in human body is still limited. Malassezia is unable to synthesize fatty acids; hence, it uptakes external fatty acids as a nutrient source for survival...
Source: Journal of Microbiology and Biotechnology - January 27, 2021 Category: Biotechnology Authors: Park M, Park S, Jung WH Tags: J Microbiol Biotechnol Source Type: research

Antiviral Treatment Reveals a Cooperative Pathogenicity of Baculovirus and Iflavirus in Spodoptera exigua, a lepidopteran insect.
Abstract The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various vegetable crops. Two infectious insect viruses, baculovirus and iflavirus, are known to induce epizootic in S. exigua populations. Indeed, some laboratory colonies appeared to be covertly infected by these viruses. Diagnostic PCR tests detected two kinds of viruses: Spodoptera exigua multiple nucleopolyhedrosis virus (SeMNPV) and iflaviruses (SeIfV1 and SeIfV2). Viral extract from dead larvae of S. exigua could infect Sf9 cells and produce occlusion bodies (OBs). Feeding OBs to asymptomatic larvae of S. exigua caused signific...
Source: Journal of Microbiology and Biotechnology - January 22, 2021 Category: Biotechnology Authors: Roy MC, Ahmed S, Mollah MMI, Kim Y Tags: J Microbiol Biotechnol Source Type: research

Development of a Novel Multiple Cross-linking Spiral Amplification for Rapid and Sensitive Detection of HPV16 DNA.
In this study, a multiple cross-linking spiral amplification (MCLSA) assay was developed for the visual detection of HPV-16. In the MCLSA assay, samples were incubated under optimized conditions at 62°C for 45 min, and after mixing with the SYBR Green I (SGI) dye, the positive amplicons showed bright green fluorescence while the negative amplicons exhibited no obvious change. The specificity test revealed that the developed MCLSA technique had high specificity and could effectively distinguish all five HPV-16 strains from other pathogenic microorganisms. In terms of analytical sensitivity, the limit of detection (LoD) ...
Source: Journal of Microbiology and Biotechnology - January 22, 2021 Category: Biotechnology Authors: Zhang D, Liu D, Liu B, Ma X Tags: J Microbiol Biotechnol Source Type: research

Genetic Background behind the Amino Acid Profiles of Fermented Soybeans Produced by Four Bacillus spp.
Abstract Strains of four Bacillus spp. were respectively inoculated into sterilized soybeans and the free amino acid profiles of the resulting soybean cultures were analyzed to discern their metabolic traits. After the 30-day cultures, B. licheniformis showed the highest production of serine, threonine, and glutamic acid; B. subtilis exhibited the highest production of alanine, asparagine, glycine, leucine, proline, tryptophan, and lysine. B. velezensis increased the γ-aminobutyric acid (GABA) concentration to>200% of that in the control samples. B. sonorensis produced somewhat similar amino acid profile ...
Source: Journal of Microbiology and Biotechnology - January 22, 2021 Category: Biotechnology Authors: Jang M, Jeong DW, Heo G, Kong H, Kim CT, Lee JH Tags: J Microbiol Biotechnol Source Type: research

Effect of pH buffer and carbon metabolism on the yield and mechanical properties of bacterial cellulose produced by Komagataeibacter hansenii ATCC 53582.
This study broadens the understanding of how carbon sources affect BC biosynthesis. PMID: 33323677 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - December 16, 2020 Category: Biotechnology Authors: Li Z, Chen SQ, Cao X, Li L, Zhu J, Yu H Tags: J Microbiol Biotechnol Source Type: research

Chikungunya virus nsP2 impairs MDA5/RIG-I-mediated induction of NF- κB promoter activation: a potential target for virus-specific therapeutics.
Chikungunya virus nsP2 impairs MDA5/RIG-I-mediated induction of NF-κB promoter activation: a potential target for virus-specific therapeutics. J Microbiol Biotechnol. 2020 Dec 16;: Authors: Bae S, Lee JY, Myoung J Abstract Chikungunya virus (CHIKV) was first identified in 1952 as a causative agent of outbreaks. CHIKV is transmitted by two mosquito species, Aedes aegypti and A. albopictus. Symptoms after CHIKV infection in human are typically fever and joint pain, but can also include headache, muscle pain, joint swelling, polyarthralgia, and rash. CHIKV is an enveloped single-stranded, positive-...
Source: Journal of Microbiology and Biotechnology - December 16, 2020 Category: Biotechnology Authors: Bae S, Lee JY, Myoung J Tags: J Microbiol Biotechnol Source Type: research

Computational identification of essential enzymes as potential drug targets in Shigella flexneri pathogenesis using metabolic pathway analysis and epitope mapping.
Abstract Shigella flexneri is a facultative intracellular pathogen that causes bacillary dysentery in humans. The organism can cause more than a million deaths yearly because of infections with Shigella flexneri and the victims are the children living in the developing countries. Therefore, identification of novel and unique drug targets of this pathogen is instrumental to overcome the problem of drug resistance to current therapy of antibiotic drugs given to the patients. In the current study, comparative metabolic pathway analysis was implemented to identify essential enzymes for the survival of the bacteria and...
Source: Journal of Microbiology and Biotechnology - December 14, 2020 Category: Biotechnology Authors: Narad P, Himanshu, Bansal H Tags: J Microbiol Biotechnol Source Type: research

High plasticity of the gut microbiome and muscle metabolome of Chinese mitten crab (Eriocheir sinensis) in diverse environments.
In this study, we conducted the integrated gut microbiome and muscle metabolome analysis on E. sinensis collected from three different environments, namely, artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of high phenotypic plasticity of E. sinensis. Our study presented three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments. Meanwhile, the metabolites associated with amino acids, fatty ac...
Source: Journal of Microbiology and Biotechnology - December 14, 2020 Category: Biotechnology Authors: Chen X, Chen H, Liu Q, Ni K, Ding R, Wang J, Wang C Tags: J Microbiol Biotechnol Source Type: research

The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells.
Abstract microRNA-361-3p (miR-361-3p) is involved in carcinogenesis of oral cancer and pancreatic catheter adenocarcinoma, and has anti-carcinogenic effects on non-small cell lung cancer (NSCLC), however, its effect on multiple myeloma (MM) is less reported. The current study found that up-regulating the expression of miR-361-3p inhibited MM cell viability and promoted MM apoptosis. Expressions of tumour necrosis factor receptor-associated factor 6 (TRAF6) and miR-361-3p of MM cells were measured. In addition, the viability, colony formation rate, apoptosis of MM cells were detected, and expressions of apoptosis-r...
Source: Journal of Microbiology and Biotechnology - December 14, 2020 Category: Biotechnology Authors: Fan Z, Wu Z, Yang B Tags: J Microbiol Biotechnol Source Type: research

Production of algal biomass production and high-value compounds mediated by the interaction of microalgal Oocystis sp. KNUA044 and bacterium Sphingomonas KNU100.
Abstract There is growing interest in the production of microalgae-based high-value by-products as an emerging green biotechnology. However, a cultivation platform for Oocystis sp. has yet to be established. To overcome this limitation, we examined the effect of bacterial culture additions on the growth and production of valuable compounds the Oocystis sp. KNUA044 microalgal strain isolated from a locally adapted region in Korea. This microalgal strain grew only in the presence of a clear supernatant of Sphingomonas sp. KNU100 culture solution and generated 28.57 mg/L/d of biomass productivity. Protein content (43...
Source: Journal of Microbiology and Biotechnology - December 14, 2020 Category: Biotechnology Authors: Na H, Jo SW, Do JM, Kim IS, Yoon HS Tags: J Microbiol Biotechnol Source Type: research

Function and molecular ecology significance of two catechol-degrading gene clusters in Pseudomonas putida ND6.
In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. Inthe presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is lik...
Source: Journal of Microbiology and Biotechnology - December 11, 2020 Category: Biotechnology Authors: Shi S, Yang L, Yang C, Li S, Zhao H, Ren L, Wang X, Lu F, Li Y, Zhao H Tags: J Microbiol Biotechnol Source Type: research

Improvement of unsaturated fatty acid production from Porphyridium cruentum using a two-phase culture system in a photobioreactor with light-emitting diodes (LEDs).
In this study, the culture conditions for Porphyridium cruentum were optimized to obtain the maximum biomass and lipid productions. The eicosapentaenoic acid content was increased by pH optimization. Porphyridium cruentum was cultured with modified F/2 medium in 14-L photo-bioreactors using a two-phase culture system, in which the green (520 nm) and red (625 nm) light-emitting diodes (LEDs) were used during the first and second phases for biomass production and lipid production, respectively. Various parameters, including aeration rate, light intensity, photoperiod, and pH were optimized. The maximum biomass concentration ...
Source: Journal of Microbiology and Biotechnology - December 7, 2020 Category: Biotechnology Authors: Kim SH, Lee UH, Lee SB, Jeong GT, Kim SK Tags: J Microbiol Biotechnol Source Type: research

Mechanisms and Control Strategies of Antibiotic Resistance in Pathological Biofilms.
Abstract Bacterial biofilm is a community of bacteria that are embedded and structured in self-secreted extracellular matrix. An important clinical-related characteristic of bacterial biofilms is that they are much more resistant to antimicrobial agents than the planktonic cells (up to 1000 times), which is one of the main causes of antibiotic resistance in clinics. Therefore, infections caused by biofilms are notoriously difficult to eradicate, such as lung infection caused by Pseudomonas aeruginosa in cystic fibrosis patients. Understanding mechanisms of resistance of biofilms will provide direct insights to how...
Source: Journal of Microbiology and Biotechnology - December 7, 2020 Category: Biotechnology Authors: Luo Y, Yang Q, Zhang D, Yan W Tags: J Microbiol Biotechnol Source Type: research

Influenza chimeric protein (3M2e-3HA2-NP) adjuvanted with PGA/alum conferred cross-protection against heterologous influenza A viruses.
In this study, we produced a 3M2e-3HA2-NP chimeric protein as a vaccine antigen candidate using an Escherichia coli expression system. The vaccination of chimeric protein (15 μg) conferred complete protection against A/Puerto Rico/8/1934 (H1N1; PR8) in mice. It strongly induced influenza virus-specific antibody responses, cytotoxic T lymphocyte activity, and antibody-dependent cellular cytotoxicity. To spare the dose and enhance the cross-reactivity of the chimeric, we used a complex of poly-γ-glutamic acid and alum (PGA/alum) as an adjuvant. PGA/alum-adjuvanted low-dose chimeric protein (1 or 5 μg) exhibited h...
Source: Journal of Microbiology and Biotechnology - December 2, 2020 Category: Biotechnology Authors: Kwak C, Nguyen QT, Kim J, Kim TH, Poo H Tags: J Microbiol Biotechnol Source Type: research

The effect of honokiol on ergosterol biosynthesis and vacuole function in Candida albicans.
Abstract Ergosterol, an essential constituent of membrane lipids of yeast, is distributed in both the cell membrane and the intracellular endomembrane system such as vacuoles. Honokiol, a major polyphenol isolated from Magnolia officinalis, has been shown to inhibit the growth of Candida albicans. Here, we assessed the effect of honokiol on ergosterol biosynthesis and vacuole function in C. albicans. Honokiol could decrease the ergosterol content and upregulate the expression of genes related with the ergosterol biosynthesis pathway. The exogenous supply of ergosterol attenuated the toxicity of honokiol against C....
Source: Journal of Microbiology and Biotechnology - November 27, 2020 Category: Biotechnology Authors: Sun L, Liao K Tags: J Microbiol Biotechnol Source Type: research

Periplanetasin-2 enhances the antibacterial properties of vancomycin or chloramphenicol in Escherichia coli.
Abstract Periplanetasin-2 from cockroach exhibits broad spectrum of antimicrobial activity. The underlying antibacterial mechanisms rely on the stimulation of reactive oxygen species overproduction to induce apoptotic cell death. A promising strategy to increase the bioavailability of periplanetasin-2 involves reducing the dose through combination therapy with other antibacterials, that show synergistic effects. Thus, the synergistic antibacterial activity of periplanetasin-2 with conventional antibacterial agents and its mechanisms was examined against Escherichia coli in this study. Among the agents tested, the ...
Source: Journal of Microbiology and Biotechnology - November 27, 2020 Category: Biotechnology Authors: Lee H, Hwang JS, Lee DG Tags: J Microbiol Biotechnol Source Type: research

Anti-endotoxin 9-meric peptide with therapeutic potential for the treatment of endotoxemia.
Abstract Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacterium can lead to severe septic shocks. With recent emergence of multidrug-resistant gram-negative bacteria as well as lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We had previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. To further exam...
Source: Journal of Microbiology and Biotechnology - November 20, 2020 Category: Biotechnology Authors: Krishnan M, Choi J, Choi S, Kim Y Tags: J Microbiol Biotechnol Source Type: research

Zika Virus NS2A and NS4A Downregulate the Promoter Activity of Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B Cells.
This study could help understand the mechanisms how ZIKV controls innate immune responses and may be provided for development of ZIKV-specific medicines. PMID: 33203823 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - November 17, 2020 Category: Biotechnology Authors: Lee JY, Nguyen TTN, Myoung J Tags: J Microbiol Biotechnol Source Type: research

Physico-chemical characterization of biosurfactant from Pseudomonas aeruginosa PU1 and its application in microbial enhance oil recovery.
Abstract Biosurfactant-producing bacteria were isolated from transformer oil-contaminated soil. The strain was identified as Pseudomonas aeruginosa PU1 based on its molecular characterization by 16S rRNA gene sequencing. The extraction of biosurfactant was done by acid precipitation method using 2 N hydrochloric acid and further purified by silica-gel column chromatography. The highest rhamnolipid biosurfactant production (8.92 ± 0.08 g/L) was obtained using molasses (6%, w/v) and ammonium nitrate (5%, w/v). The purified biosurfactant showed a reduction of surface tension of water from 70.23 mN/m to 29.77 m...
Source: Journal of Microbiology and Biotechnology - November 17, 2020 Category: Biotechnology Authors: Domdi L, Lakra AK, Tilwani YM, Arul V Tags: J Microbiol Biotechnol Source Type: research

Production of Polyhydroxyalkanoates (PHA) by Haloferax mediterranei from Food Waste Derived Nutrients for Biodegradable Plastic Applications.
This study aimed to utilize food waste as low-cost feedstock to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by Haloferax mediterranei . The effects of acetate (Ac), propionate (Pr), butyrate (Bu), and the short-chain carboxylates derived from food waste were examined on the microbial growth and PHBV production. Results showed that a mixture of carboxylates provided a 55% higher PHBV yield than glucose. The food-waste-derived nutrients achieved the yields of 0.41 to 0.54 g PHBV/g Ac from initial loadings of 450 mg/L to 1800 mg/L Ac of total carboxylates. And the consumption of individual carboxylate varied b...
Source: Journal of Microbiology and Biotechnology - November 17, 2020 Category: Biotechnology Authors: Wang K, Zhang R Tags: J Microbiol Biotechnol Source Type: research

The first report to evaluate safety of cyanobacterium Leptolyngbya sp. KIOST-1 for use as a food ingredient: Oral acute toxicity and genotoxicity study.
Abstract Leptolyngbya sp. KIOST-1 (LK1) is a newly isolated cyanobacterium that shows no obvious cytotoxicity and contains high protein content for both human and animal diets. However, only limited information is available on its toxic effects. The purpose of this study was to validate the safety of LK1 powder. Following Organisation for Economic Co-operation and Development (OECD) guidelines, a single-dose oral toxicity test in Sprague Dawley rats was performed. Genotoxicity was assessed using a bacterial reverse mutation test with Salmonella typhimurium (strains TA98, TA100, TA1535, and TA1537) and Escherichia ...
Source: Journal of Microbiology and Biotechnology - November 17, 2020 Category: Biotechnology Authors: Lee Y, Kim T, Lee WK, Ryu YK, Kim JH, Jeong Y, Park A, Lee YJ, Oh C, Kang DH Tags: J Microbiol Biotechnol Source Type: research

Methylglyoxal-scavenging enzyme activities trigger erythroascorbate peroxidase and cytochrome c peroxidase in glutathione-depleted Candida albicans.
Abstract γ-glutamylcysteine synthetase (Gcs1) and glutathione reductase (Glr1) activity maintains minimal levels of cellular methylglyoxal in Candida albicans. In glutathione-depleted Δgcs1, we previously saw that NAD(H)-linked methylglyoxal oxidoreductase (Mgd1) and alcohol dehydrogenase (Adh1) are the most active methylglyoxal scavengers. With methylglyoxal accumulation, disruptants lacking MGD1 or ADH1 exhibit a poor redox state. However, there is little convincing evidence for a reciprocal relationship between methylglyoxal scavenger genes-disrupted mutants and changes in glutathione-(in)dependent ...
Source: Journal of Microbiology and Biotechnology - November 16, 2020 Category: Biotechnology Authors: Kang SO, Kwak MK Tags: J Microbiol Biotechnol Source Type: research

Chromophorylation of a novel cyanobacteriochrome GAF domain from Spirulina and its response to copper ions.
This study identified a novel PEB-binding cyanobacteriochrome-based fluorescent protein with the highest quantum yield reported to date and indicated its potential as a biosensor for the rapid detection of copper ions. PMID: 33203817 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - November 14, 2020 Category: Biotechnology Authors: Jiang SD, Sheng Y, Wu XJ, Zhu YL, Li PP Tags: J Microbiol Biotechnol Source Type: research

Volatile metabolic markers for monitoring Pectobacterium carotovorum subsp. carotovorum using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry.
In this study, we used headspace solid-phase microextraction followed by gas chromatography coupled with mass spectrometry (HS-SPME-GC-MS) to identify volatile compounds (VCs) in PCC-inoculated cabbage at different storage temperatures. HS-SPME-GC-MS allowed for recognition of extracellular metabolites in PCC-infected cabbages by identifying specific volatile metabolic markers. 4-Ethyl-5-methylthiazole and 3-butenyl isothiocyanate were identified as markers of fresh cabbages, whereas 2,3-butanediol and ethyl acetate were identified as markers of soft rot in PCC-infected cabbages. These analytical results demonstrate a suit...
Source: Journal of Microbiology and Biotechnology - November 14, 2020 Category: Biotechnology Authors: Yang JS, Lee HW, Song H, Ha JH Tags: J Microbiol Biotechnol Source Type: research

Pine needle extract applicable to topical treatment for the prevention of human papillomavirus infection.
In conclusion, PN-MC represents a potential topical virucide for HPV infection. PMID: 33203819 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - November 10, 2020 Category: Biotechnology Authors: Lee HJ, Park M, Choi H, Nowakowska A, Moon C, Kwak JH, Kim YB Tags: J Microbiol Biotechnol Source Type: research

Antioxidant and Antibacterial Activity of Caprylic Acid Vanillyl Ester Produced by Lipase-Mediated Transesterification.
In this study, the antibacterial activity of vanillyl ester with medium chain fatty acid was first revealed. Zeta potential measurements confirmed that BAVE and CAVE were inserted into B. coagulans membrane. In addition, the propidium iodide uptake assay and fluorescent microscopy showed that CAVE increased B. coagulans membrane permeability. Therefore, CAVE is expected to play an important role in the food and cosmetics industries as a bi-functional material with both antioxidant and antibacterial activities. PMID: 33203820 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - November 10, 2020 Category: Biotechnology Authors: Kim JJ, Kim HK Tags: J Microbiol Biotechnol Source Type: research

Repurposing screens of FDA-approved drugs identify 29 inhibitors of SARS-CoV-2.
We report this new list of inhibitors to quickly provide basic information for consideration in developing potential therapies. PMID: 33203821 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - November 10, 2020 Category: Biotechnology Authors: Ku KB, Shin HJ, Kim HS, Kim BT, Kim SJ, Kim C Tags: J Microbiol Biotechnol Source Type: research

NaCl concentration-dependent aminoglycoside resistance of Halomonas socia CKY01 and identification of related genes.
Abstract Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, there was not much information on antibiotic resistance of Halomonas species. As an effort to find antibiotic resistance of Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics, including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resist...
Source: Journal of Microbiology and Biotechnology - November 4, 2020 Category: Biotechnology Authors: Park YL, Choi TR, Kim HJ, Song HS, Lee HS, Park SL, Lee SM, Kim SH, Park S, Bhatia SK, Gurav R, Sung C, Seo SO, Yang YH Tags: J Microbiol Biotechnol Source Type: research

Aucklandia lappa causes membrane permeation of Candida albicans.
Abstract Candida albicans is a major fungal pathogen in humans. In our previous study, we reported that an ethanol extract from Aucklandia lappa weakens C. albicans cell wall by inhibiting synthesis or assembly of both (1,3)-β-D-glucan polymers and chitin. In the current study, we found that the extract is involved in permeabilization of C. albicans cell membranes. While uptake of ethidium bromide (EtBr) was 3.0% in control cells, it increased to 7.4% for 30 min in the presence of the A. lappa ethanol extract at its minimal inhibitory concentration (MIC), 0.78 mg/mL, compared to uptake by heat-killed cells. B...
Source: Journal of Microbiology and Biotechnology - November 4, 2020 Category: Biotechnology Authors: Lee HS, Kim Y Tags: J Microbiol Biotechnol Source Type: research