Draft genome analysis of antimicrobial Streptomyces isolated from Himalayan lichen.
In conclusion, we expect that lichen-associated bacteria from the Himalayas can produce new secondary metabolites, and we found several secondary metabolite-related biosynthetic gene clusters to support this hypothesis. PMID: 31288301 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - July 10, 2019 Category: Biotechnology Authors: Kim B, Han SR, Lamichhane J, Park H, Oh TJ Tags: J Microbiol Biotechnol Source Type: research

Potential Antimicrobial Applications of Chitosan Nanoparticles (ChNP).
Abstract Polymeric nanoparticles are widely used for drug delivery due to its biodegradability property. Among the wide array of polymer, chitosan has received growing interest among researchers. It was widely used as a vehicle in polymeric nanoparticles for drug targeting. This review explored the current research on the antimicrobial activity of chitosan nanoparticles (ChNP) and the impact on the clinical applications. The antimicrobial activities of ChNP were widely reported against bacteria, fungi, yeasts and algae, on both in vivo and in vitro studies. For pharmaceutical applications, ChNP was used as antimic...
Source: Journal of Microbiology and Biotechnology - July 10, 2019 Category: Biotechnology Authors: Rozman NAS, Tong WY, Leong CR, Tan WN, Hasanolbasori MA, Abdullah SZ Tags: J Microbiol Biotechnol Source Type: research

Comparison of the Stability of Poly- γ-glutamate Hydrogels Prepared by UV and γ-ray Irradiation.
This study was conducted to enabling the γ-PGA hydrogel to maintain a stable form in solutions. The γ-PGA mixture for UV-irradiation was prepared with a cross-linker (N,N,N-trimethyl-3-[(2-methylacryloyl)amino]propan-1-aminium). Both γ-PGA hydrogels' characteristics, including stability in solutions, were examined. The UV irradiated γ-PGA hydrogel maintained a stable form during the nine weeks of the study, but the γ-ray irradiated hydrogel dissolved after one week. PMID: 31280528 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Park SJ, Uyama H, Kwak MS, Sung MH Tags: J Microbiol Biotechnol Source Type: research

Development of an Equine Antitoxin by Immunizing the Halla Horse with the Receptor-Binding Domain of Botulinum Neurotoxin Type A1.
Abstract Botulinum neurotoxins (BoNTs), produced by Clostridium botulinum, are the most toxic substances known. However, the number of currently approved medical countermeasures for these toxins is very limited. Therefore, studies on therapeutic antitoxins are essential to prepare for toxin-related emergencies. Currently, more than 10,000 Halla horses, a crossbreed between the native Jeju and Thoroughbred horses, are being raised in Jeju Island of Korea. They can be used for equine antitoxin experiments and production of hyperimmune serum against BoNT/A1. Instead of the inactivated BoNT/A1 toxoid, Halla horse was ...
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Kim NY, Park KE, Lee YJ, Kim YM, Hong SH, Son WR, Hong S, Lee S, Ahn HB, Yang J, Seo JP, Lim YK, Yu CH, Hur GH, Jeong ST, Lee HS, Song K, Kang TJ, Shin YK, Choi JY, Choi JS Tags: J Microbiol Biotechnol Source Type: research

Protective effect of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against hydrogen peroxide-induced oxidative stress in in vitro and in vivo model zebrafish.
Abstract We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly inhibited the cleavage of caspase-9 and PARP and reduced Bax level...
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Cho SH, Heo SJ, Yang HW, Ko EY, Jung MS, Cha SH, Ahn G, Jeon YJ, Kim KN Tags: J Microbiol Biotechnol Source Type: research

Optimized Production of Poly( γ-glutamic acid) By Bacillus sp. FBL-2 Through Response Surface Methodology Using Central Composite Design.
Optimized Production of Poly(γ-glutamic acid) By Bacillus sp. FBL-2 Through Response Surface Methodology Using Central Composite Design. J Microbiol Biotechnol. 2019 Jul 09;: Authors: Min JH, Reddy LV, Dimitris C, Kim YM, Wee YJ Abstract In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp. FBL-2 was studied using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield...
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Min JH, Reddy LV, Dimitris C, Kim YM, Wee YJ Tags: J Microbiol Biotechnol Source Type: research

Effect of Differential Thermal Drying Conditions on the Immunomodulatory Function of Ginger.
Abstract Thermal drying is a common process used in the food industry for the modification of agricultural products. However, whilst various studies have investigated the alteration in physiochemical properties and chemical composition after drying, research focusing on the relationship between different dehydration conditions and bioactivity are scarce. In the current study, we prepared dried ginger under nine different conditions by varying the processing time and temperature and compared their immunomodulatory effects. Interestingly, depending on the drying condition, there were significant differences in the i...
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Lee JS, Kim B, Kim JH, Jeong M, Lim S, Byun S Tags: J Microbiol Biotechnol Source Type: research

Elucidation of the Inhibitory Mechanisms of Nipponoparmelia laevior Lichen Extract against Influenza A (H1N1) Virus through Proteomic Analyses.
Abstract Lichens contain diverse bioactive secondary metabolites with various chemical and biological properties, which have been widely studied. However, details of the inhibitory mechanisms of their secondary metabolites against influenza A virus (IAV) have not been documented. Here, we investigated the antiviral effect of lichen extracts, obtained from South Korea, against IAV in the MDCK cells. Of the lichens tested, Nipponoparmelia laevior (LC24) exhibited the most potent inhibitory effect against IAV infection. LC24 extract significantly increased cell viability, and reduced apoptosis in IAV-infected cells. ...
Source: Journal of Microbiology and Biotechnology - July 9, 2019 Category: Biotechnology Authors: Tran CV, Cho SY, Kwon J, Kim D Tags: J Microbiol Biotechnol Source Type: research

Induced tolerance to salinity stress by halotolerant bacteria Bacillus aryabhattai H19-1 and B. mesonae H20-5 in tomato plants.
In this study, we investigated the effects of two selected strains, Bacillus aryabhattai H19-1 and B. mesonae H20-5, on responses of tomato plants against salinity stress. As a result, they alleviated decrease in plant growth and chlorophyll content; only strain H19-1 increased carotenoid content compared to that in untreated plants under salinity stress. Strains H19-1 and H20-5 significantly decreased electrolyte leakage, whereas increased Ca2+ content compared to that in the untreated control. Our results also indicated that H20-5-treated plants accumulated significantly higher levels of proline, abscisic acid (ABA), and...
Source: Journal of Microbiology and Biotechnology - June 20, 2019 Category: Biotechnology Authors: Yoo SJ, Weon HY, Song J, Sang MK Tags: J Microbiol Biotechnol Source Type: research

Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF- κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells.
Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells. J Microbiol Biotechnol. 2019 Jun 20;: Authors: Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD Abstract Probiotics are known to provide the host with immune-modulatory effects and are therefore of remarkable interest for therapeutic and prophylactic applications against various disorders, including inflammatory diseases. Weissella cibaria JW15 (JW15) has been reported to possess probiotic and antioxidant properties. However, t...
Source: Journal of Microbiology and Biotechnology - June 20, 2019 Category: Biotechnology Authors: Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD Tags: J Microbiol Biotechnol Source Type: research

Development of a redox dye-based rapid colorimetric assay for the quantitation of viability/mortality of pine wilt nematode.
Abstract Control of pine wilt disease, which is caused by pine wilt nematode, Bursaphelenchus xylophilus, is heavily dependent on the use of chemicals such as abamectin. Although such chemicals are highly effective, demands for alternatives that are derived preferentially from natural sources, are increasing out of environmental concerns. One of the challenges to discovery of alternative control agents is lack of fast and efficient screening method that can be used in high-throughput manner. Here we described the development of colorimetric assay for the rapid and accurate screening of candidate nematicidal compou...
Source: Journal of Microbiology and Biotechnology - June 20, 2019 Category: Biotechnology Authors: Han K, Lee J, Shanmugam G, Lee SK, Jeon J Tags: J Microbiol Biotechnol Source Type: research

A laboratory-scale study of the applicability of a halophilic sediment bioelectrochemical system for in situ reclamation of water and sediment in brackish aquaculture ponds: establishment, bacterial community and performance evaluation.
In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in-situ treatment of the water and the sediment of brackish aquaculture ponds polluted with uneaten feed. A SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. Such a tank (test tank) and the control (not containing the SBES) were fed with shrimp feed in a scheme that mimics a situation where 50% of feed is uneaten. After the SBES was inoculated with microbial sources from actual shrimp pond sediments, electricity generation was well observed from the first experimental week...
Source: Journal of Microbiology and Biotechnology - June 20, 2019 Category: Biotechnology Authors: Pham HT, Tran HT, Vu LT, Dang HT, Nguyen TTT, Dang THT, Nguyen MTT, Nguyen HQ, Kim HB Tags: J Microbiol Biotechnol Source Type: research

Heat-killed Lactobacillus plantarum KCTC 13314BP enhances phagocytic activity and immunomodulatory effects via activation of MAPK and STAT3 pathways.
Abstract Identification of novel probiotic strains is of great interest in the field of functional foods. Specific strains of heat-killed bacteria have been reported to exert immunomodulatory effects. Herein, we investigated the immune-stimulatory function of heat-killed Lactobacillus plantarum KCTC 13314BP (LBP). Treatment with LBP significantly increased the production of TNF-α and IL-6 by macrophages. More importantly, LBP was able to enhance the phagocytic activity of macrophages against bacterial particles. Activation of p38, JNK, ERK, NF-κB, and STAT3 was involved in the immunomodulatory function...
Source: Journal of Microbiology and Biotechnology - June 18, 2019 Category: Biotechnology Authors: Jeong M, Kim JH, Yang H, Kang SD, Song S, Lee D, Lee JS, Park JHY, Byun S, Lee KW Tags: J Microbiol Biotechnol Source Type: research

Purification and Characterization of a Bacteriocin, BacBS2, Produced by Bacillus velezensis BS2 Isolated from Meong-ge Jeotgal.
Abstract Bacillus velezensis BS2 was isolated from Meong-ge (common sea squirt) jeotgal, a Korean fermented seafood, and produces a bacteriocin, BacBS2, which strongly inhibits Listeria monocytogenes and Bacillus cereus. BacBS2 was partially purified by Q-Sepharose column chromatography after ammonium sulfate precipitation of culture supernatant, then further purified by Sephadex G-50 column chromatography. Partially purified BacBS2 was estimated to be 6.5 kDa in size by Tricine-SDS PAGE and activity detection by gel-overlay. Enzyme treatment and FT-IR spectrum of partially purified BacBS2 confirmed its proteinaci...
Source: Journal of Microbiology and Biotechnology - June 18, 2019 Category: Biotechnology Authors: Perumal V, Yao Z, Kim JA, Kim HJ, Kim JH Tags: J Microbiol Biotechnol Source Type: research

Programmed Cell Death in Bacterial Community: Mechanisms of Action, Causes and Consequences.
Abstract In the bacterial community, unicellular organisms act as a multicellular being. Bacteria interact within the community and the programmed cell death (PCD) in prokaryotes is one of the altruistic actions for the whole population to thrive. Genetically, encoded cell death pathways are triggered by DNA damage or nutrient starvation. Given the environmental and bacterial diversity, different PCD mechanisms are operated. Still, their biochemical and physiological aspects remain unrevealed. There are three main pathways; thymineless death, apoptosis-like death, and toxin-antitoxin system. The discovery of the P...
Source: Journal of Microbiology and Biotechnology - June 18, 2019 Category: Biotechnology Authors: Lee H, Lee DG Tags: J Microbiol Biotechnol Source Type: research

Influence of the culture conditions on the production of NGPs by Aspergillus tubingensis.
In conclusion, this study can contribute to our knowledge regarding A. tubingensis to present an effective method to increase NGPs's production, which may support the development of current industrial processes. PMID: 31216791 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - June 18, 2019 Category: Biotechnology Authors: Lopez de Leon L, Caceres I, Bornot J, Choque E, Raynal J, Taillandier P, Mathieu F Tags: J Microbiol Biotechnol Source Type: research

Enhancing butyrate production, ruminal fermentation and microbial population through supplementation with Clostridium saccharobutylicum.
Abstract Butyrate is known to play a significant role in energy metabolism and regulating genomic activities which influences rumen nutrition utilization and function. Thus, this study investigated the effects of an isolated butyrate-producing bacteria, Clostridium saccharobutylicum, in rumen butyrate production, fermentation parameters and microbial population in Holstein-Friesian cow. An isolated butyrate-producing bacterium from the ruminal fluid of a Holstein-Friesian cow was identified and characterized as Clostridium saccharobutylicum RNAL841125 using 16S rRNA gene sequencing and phylogenetic analyses. The b...
Source: Journal of Microbiology and Biotechnology - June 13, 2019 Category: Biotechnology Authors: Miguel MA, Lee SS, Mamuad LL, Choi YJ, Jeong CD, Son A, Cho KK, Kim ET, Kim SB, Lee SS Tags: J Microbiol Biotechnol Source Type: research

Dose-dependent Inhibition of Melanoma Differentiation-Associated Gene 5-mediated Activation of Type I Interferon Responses by Methyltransferase of Hepatitis E Virus.
Abstract Hepatitis E virus (HEV) accounts for 20 million infections in humans worldwide. In most cases, the infections are self-limiting while HEV genotype 1 infection cases may lead to lethal infections in pregnant women (~ 20% fatality). The lack of small animal models has hampered detailed analysis of virus-host interactions and HEV-induced pathology. Here, by employing a recently developed culture-adapted HEV, we demonstrated that methyltransferase, a non-structural protein, strongly inhibits melanoma differentiation-associated gene 5 (MDA5)-mediated activation of type I interferon responses. Compared to uninf...
Source: Journal of Microbiology and Biotechnology - June 12, 2019 Category: Biotechnology Authors: Myoung J, Min KS Tags: J Microbiol Biotechnol Source Type: research

Gallium(III) Nitrate Inhibits Pathogenic Vibrio splendidus Vs by Interfering with the Iron Uptake Pathway.
In conclusion, our study suggested that although Ga(NO3)3 could interfere with the growth of V. splendidus Vs, it could also stimulate both the production of Fe3+-reducing HGA-melanin and the expression of feoA and feoB , which facilitate Fe2+ transport in V. splendidus Vs. PMID: 31216793 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - June 10, 2019 Category: Biotechnology Authors: Song T, Zhao X, Shao Y, Guo M, Li C, Zhang W Tags: J Microbiol Biotechnol Source Type: research

Reliable Identification of Bacillus cereus Group Species Using Low Mass Biomarkers by MALDI-TOF MS.
In this study, to overcome the limits of the current MALDI-TOF MS identification method using ribosomal protein spectra, we applied MALDI-TOF MS of low-mass profiling to the identification of two genetically related Bacillus species, the food-borne pathogen Bacillus cereus, and the insect pathogen Bacillus thuringiensis. The mass spectra of small molecules from 17 type strains of two bacilli were compared to the morphological, biochemical, and genetic identification methods of pathogens. The specific mass peaks in the low-mass range (m/z 500- 3,000) successfully identified various closely-related strains belonging to these...
Source: Journal of Microbiology and Biotechnology - June 7, 2019 Category: Biotechnology Authors: Ha M, Jo HJ, Choi EK, Kim Y, Kim J, Cho HJ Tags: J Microbiol Biotechnol Source Type: research

Comparative Genomics Approaches to Understanding Virulence and Antimicrobial Resistance of Salmonella Typhimurium ST1539 Isolated from a Poultry Slaughterhouse in Korea.
In this study, comparative genomics approaches were applied to two S. Typhimurium strains, ST1539 and ST1120, isolated from a duck slaughterhouse and a pig farm, respectively, to characterize their virulence and antimicrobial resistance-associated genomic determinants. ST1539 containing a chromosome (4,905,039 bp; 4,403 CDSs) and a plasmid (93,876 bp; 96 CDSs) was phylogenetically distinct from other S. Typhimurium strains such as ST1120 and LT2. Compared to the ST1120 genome (previously deposited in GenBank; CP021909.1 and CP021910.1), ST1539 possesses more virulence determinants, including ST64B prophage, plasmid spv ope...
Source: Journal of Microbiology and Biotechnology - June 4, 2019 Category: Biotechnology Authors: Kim E, Park S, Cho S, Hahn TW, Yoon H Tags: J Microbiol Biotechnol Source Type: research

Synthetic bacteria for therapeutics: applications of synthetic systems.
Abstract Synthetic biology builds programmed biological systems for a wide range of purposes such as improving human health, remedying the environment, and boosting the production of valuable chemical substances. In recent years, the rapid development of synthetic biology has enabled synthetic bacterium-based diagnoses and therapeutics superior to traditional methodologies by engaging bacterial sensing of and response to environmental signals inherent in these complex biological systems. Biosynthetic systems have opened a new avenue of disease diagnosis and treatment. In this review, we introduce designed syntheti...
Source: Journal of Microbiology and Biotechnology - June 4, 2019 Category: Biotechnology Authors: Vo PNL, Lee HM, Na D Tags: J Microbiol Biotechnol Source Type: research

Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coli.
Abstract Anthranilate derivates are used as flavoring and fragrant agents for a long period time. Recently, these compounds gain an attention due to the new biological functions including antinociceptive and analgesic activities. Three anthranilate derivatives, N-methylanthranilate, methyl anthrannilate, and methyl N-methylanthranilate were synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and PqsA encoding anthranilate Coenzyme A ligase from Pseudomo...
Source: Journal of Microbiology and Biotechnology - June 4, 2019 Category: Biotechnology Authors: Lee HL, Kim SY, Kim EJ, Han DY, Kim BG, Ahn JH Tags: J Microbiol Biotechnol Source Type: research

Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects.
Abstract Gamma-aminobutyric acid (GABA)-producing strains were isolated from four edible insects and subjected to 16S rRNA sequence analysis. Among the four GABA-producing bacteria, Enterococcus avium JS-N6B4 exhibited the highest GABA-production, and cultivation temperature, initial pH, aerobic condition, and mono-sodium glutamate (MSG) feeding were found to be the key factors affecting GABA production rate. The culture condition was optimized in terms of glucose, yeast extract, and MSG concentrations using the response surface methodology (RSM). GABA production up to 16.64 g/L was obtained under the conditions 7...
Source: Journal of Microbiology and Biotechnology - June 4, 2019 Category: Biotechnology Authors: Jo MH, Hong SJ, Lee HN, Ju JH, Park BR, Lee JH, Kim SA, Eun JB, Wee YJ, Kim YM Tags: J Microbiol Biotechnol Source Type: research

Structure and function of the influenza A virus non-structural protein 1.
Abstract The influenza A virus is a highly infectious respiratory pathogen and can cause respiratory disease annually. To prevent surges of this virus infection, an understanding of the characteristics of virus-host interaction and development of an anti-viral agent is urgently needed. The influenza A virus can infect mammalian species including humans, pigs, horses and seals. Furthermore, this virus can switch hosts and form a novel lineage. This so-called zoonotic infection provides an opportunity for virus adaptation to the new host and leads to pandemic. Most influenza A viruses express proteins that antagoniz...
Source: Journal of Microbiology and Biotechnology - June 4, 2019 Category: Biotechnology Authors: Han CW, Jeong MS, Jang SB Tags: J Microbiol Biotechnol Source Type: research

Generation of Full-length Infectious cDNA Clones of Middle East Respiratory Syndrome Coronavirus.
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia in 2012 and its infection has been reported over 20 countries. Roughly 10,000 human cases were reported and fatality reaches up to 40%. The majority of cases occurred in Saudi Arabia and mostly sporadic outside the country except the outbreak in South Korea in 2015. The Korean MERS-CoV isolate was isolated from the second Korean patient and its genome was fully sequenced and deposited. To develop virus-specific protective and therapeutic agents against the Korean isolate and to investigate molecular determinants o...
Source: Journal of Microbiology and Biotechnology - June 3, 2019 Category: Biotechnology Authors: Lee JY, Bae S, Myoung J Tags: J Microbiol Biotechnol Source Type: research

Application of the Dynamic Regulation to Increase L-Phenylalanine Production in Escherichia coli.
Abstract Current strategies of strain improvement processes are mainly focused on enhancing the synthetic pathway of the products. However, excessive metabolic flux often creates metabolic imbalances, which leads to growth retardation and ultimately limit the yield of the product. To solve this problem, we applied a dynamic regulation strategy to produce L-phenylalanine (L-Phe) in Escherichia coli. Firstly, we constructed a series of Phe-induced promoters that exhibited different strengths through modification of the promoter region of tyrP. Then two engineered promoters were separately introduced into a Phe-produ...
Source: Journal of Microbiology and Biotechnology - May 27, 2019 Category: Biotechnology Authors: Wu J, Liu Y, Zhao S, Sun J, Jin Z, Zhang D Tags: J Microbiol Biotechnol Source Type: research

A Novel miR-1958 Is a Promoter towards Mtb Survival in RAW264.7 Cells by Inhibiting Autophagy via Atg5.
In this study, we found miR-1958, a new miRNA that could regulate autophagy by interacting with 3'UTR of autophagy-related gene 5 (Atg5). In addition, Mtb infection triggered miR-1958 expression in RAW264.7 cells. What's more, miR- 1958 overexpression blocked autophagic flux by impairing fusion of autophagosome and lysosome. Over expression of miR-1958 reduced Atg5 expression and LC3 puncta while inhibition of miR-1958 brought an increase of Atg5 and LC3 puncta; opposite results were observed in detection of p62. The survival of Mtb in RAW264.7 cells transfected with mimic of miR-1958 was enhanced. Taken together, our rese...
Source: Journal of Microbiology and Biotechnology - May 27, 2019 Category: Biotechnology Authors: Ding S, Qu Y, Yang S, Zhao Y, Xu G Tags: J Microbiol Biotechnol Source Type: research

Brewing rutin-enriched lager beer with buckwheat malt as adjuncts.
Abstract Brewing with buckwheat as an ingredient has been proven to be successful in several previous studies. However, few studies have focused on the effects of buckwheat on the rutin content and antioxidant activity of beer. In order to develop a lager beer with high rutin content and desirable sensory characteristics, tartary buckwheat malt was used as a brewing adjunct. The results showed that the rutin-degrading enzyme was the key factor affecting the rutin content in the wort and beer. Compared to beer made using the common mashing method, the rutin content in the buckwheat beers produced using an improved ...
Source: Journal of Microbiology and Biotechnology - May 24, 2019 Category: Biotechnology Authors: Deng Y, Lim J, Lee GH, Nguyen TTH, Xiao Y, Piao M, Kim D Tags: J Microbiol Biotechnol Source Type: research

Isolation and characterization of novel Chlorella species with cold resistance and high lipid accumulation for biodiesel production.
In this study, two novel Chlorella strains (ABC-002, ABC-008) were isolated from a freshwater lake in South Korea during the winter season and were examined for possible use in the biofuel production process. The comparison of ABC-002 and ABC-008 strains with Chlorella vulgaris UTEX265 under two different temperatures (10 °C, 25 °C) revealed their cold-tolerant phenotypes as well as high biomass yields. The maximum quantum yields of UTEX265, ABC-002, and ABC-008 at 10 °C were 0.5594, 0.6747, and 0.7150, respectively, providing evidence of the relatively higher cold resistance capabilities of these two strains. ...
Source: Journal of Microbiology and Biotechnology - May 24, 2019 Category: Biotechnology Authors: Koh HG, Kang NK, Kim EK, Suh WI, Park WK, Lee B, Chang YK Tags: J Microbiol Biotechnol Source Type: research

Immobilization and Performance of Penicillin G Acylase on Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO Nanocomposites.
Abstract Magnetic Ni0.7Co0.3Fe2O4 nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites, on which Penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and 45 °C while immobilized PGA's activities reached the peak at pH 7.5 and 45 °C. immobilized PGA had better thermal stability than Free PGA at range of 30-50 °C for d...
Source: Journal of Microbiology and Biotechnology - May 24, 2019 Category: Biotechnology Authors: Lv Z, Yu Q, Wang Z, Liu R Tags: J Microbiol Biotechnol Source Type: research

Comparison of Bioethanol Production by Candida molischiana and Saccharomyces cerevisiae from Glucose, Cellobiose, and Cellulose.
In this study, we compared the production of bioethanol by Candida molischiana and Saccharomyces cerevisiae at different initial concentrations of cellobiose and glucose. The results showed that C. molischiana can utilize both glucose and cellobiose, whereas S. cerevisiae can only utilize glucose. The ethanol yields were 43-51% from different initial concentrations of carbon source. In addition, different concentrations of microcrystalline cellulose (Avicel) were directly converted to ethanol by a combination of Trichoderma reesei and two yeasts. Cellulose was first hydrolyzed by a fully enzymatic saccharification process ...
Source: Journal of Microbiology and Biotechnology - May 24, 2019 Category: Biotechnology Authors: Zheng J, Negi A, Khomlaem C, Kim BS Tags: J Microbiol Biotechnol Source Type: research

Polyaromatic resin HP-20 induced accumulation of intermediate azaphilones in Monascus purpureus ΔmppC and Δmpp7 strains.
This study demonstrates the possibility that extractive fermentation with Diaion HP-20 resin can be used to obtain cryptic azaphilone metabolites. PMID: 31091861 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Lim YJ, Lee DW, Choi JJ, Park SH, Kwon HJ Tags: J Microbiol Biotechnol Source Type: research

Synthetic biology tools for novel secondary metabolite discovery in Streptomyces.
Abstract Streptomyces are attractive microbial cell factories that have industrial capability to produce a wide array of bioactive secondary metabolites. However, the genetic potential of the Streptomyces species has not been fully utilized because most of their secondary metabolite biosynthetic gene clusters (SM-BGCs) are silent under laboratory culture conditions. In an effort to activate SM-BGCs encoded in Streptomyces genomes, synthetic biology has emerged as a robust strategy to understand, design, and engineer the biosynthetic capability of Streptomyces secondary metabolites. In this regard, diverse syntheti...
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Lee N, Hwang S, Lee Y, Cho S, Palsson B, Cho BK Tags: J Microbiol Biotechnol Source Type: research

Lactobacillus casei Zhang prevents jejunal epithelial damage to early-weaned piglets induced by Escherichia coli K88 via regulation of intestinal mucosal integrity, tight junction proteins and immune factor expression.
Abstract Farm animals such as piglets are often affected by environmental stress, which can disturb the gut ecosystem. Antibiotics were commonly used to prevent diarrhea in weaned piglets, but this was banned by the European Union due to the development of antibiotic resistance. However, the use of probiotics instead of antibiotics may reduce the risk posed by pathogenic microorganisms and reduce the incidence of gastrointestinal diseases. Therefore, this study was conducted to investigate the effects of Lactobacillus casei Zhang on the mechanical barrier and immune function of early-weaned piglets infected using ...
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Wang Y, Yan X, Zhang W, Liu Y, Han D, Teng K, Ma Y Tags: J Microbiol Biotechnol Source Type: research

Synthesis of thienopyrimidine derivatives as inhibitors of STAT3 activation induced by IL-6.
Abstract A series of thienopyrimidine compounds (6Aa-g and 6Ba-d) were synthesized and characterized by NMR spectroscopy and mass spectrometry. These compounds (6Aa-g and 6Ba-d) potently inhibited STAT3 expression induced by IL-6 in a dose-dependent manner with IC50 values of 5.73-0.32 μΜ. Among the prepared thienopyrimidine derivatives, 6Aa, 6Ab, 6Ba and 6Bc significantly suppressed the phosphorylation of STAT3 and ERK1/2 stimulated by IL-6 in Hep3B cells. Furthermore, the synthesized compounds might be useful remedies for the treatment of inflammatory diseases by inhibiting the action of IL-6. PMID: 3...
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Jang HJ, Kim SM, Rho MC, Lee SW, Song YH Tags: J Microbiol Biotechnol Source Type: research

Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus.
This report provided a method and P. italicum-specific primers, which might greatly contribute to citrus postharvest industry. PMID: 31091865 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Chen K, Tian Z, Jiang F, Long CA Tags: J Microbiol Biotechnol Source Type: research

Nano-encapsulation of plant growth promoting rhizobacteria and their metabolites using alginate-silica nanoparticles and carbon nanotube improves UCB1 pistachio micropropagation.
Abstract UCB-1 is the commercial rootstock of pistachio. Reproduction of this rootstock by tissue culture is limited by low levels of proliferation rate. Therefore, any compound that improves the proliferation rate and the quality of the shoots can be used in the process of commercial reproduction of this rootstock. Use of plant growth promoting bacteria is one of the best ideas. Given the beneficial effects of nanoparticles in enhancement of the growth in plant tissue cultures, the aim of the present study was to investigate the effects of nanoencapsulation of plant growth promoting rhizobacteria (using silica na...
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Moradipour M, Saberi-Riseh R, Mohammadinejad R, Hosseini A Tags: J Microbiol Biotechnol Source Type: research

Isolation, Cloning and Co-expression of lipase and foldase genes of Burkholderia territorii GP3 from Mount Papandayan Soil.
Abstract Lipases are industrial enzymes that catalyze both triglyceride hydrolysis and ester synthesis. The overexpression of lipase gene is considered as one of the best approach to increase the enzymatic production for industrial applications. Subfamily I.2. lipases require a chaperone or foldase in order to become a fully-activated enzyme. The goal of this research was to isolate, clone, and co-express genes that encode lipase and foldase from Burkholderia territorii GP3, a lipolytic bacterial isolate obtained from Mount Papandayan soil via growth on Soil Extract Rhodamine Agar. Genes that encode for lipase (li...
Source: Journal of Microbiology and Biotechnology - May 14, 2019 Category: Biotechnology Authors: Putra L, Natadiputri GH, Meryandini A, Suwanto A Tags: J Microbiol Biotechnol Source Type: research

Erratum to: Orally-Administered Bifidobacterium animalis subsp. lactis Strain BB12 on Dextran Sodium Sulfate-Induced Colitis in Mice.
Abstract This erratum is being published to correct the author's contribution of above manuscript by Chae et al. that was published in Journal of Microbiology and Biotechnology (2018, 28:11, 1800-1805). The ninth author (Jin Hyup Lee) should be marked as corresponding author (*) with latest author (Young Jun Kim). The correspondence should appear as: *Corresponding authors Young Jun Kim Phone: +82-44-860-1435; Fax: +82-44-860-1586; E-mail: yk46@korea.ac.kr Jin Hyup Lee Phone: +82-44-860-1437 Fax: +82-44-860-1586; E-mail: jinhyuplee@korea.ac.kr. PMID: 31048595 [PubMed - in process] (Source: Journal of Microbio...
Source: Journal of Microbiology and Biotechnology - April 28, 2019 Category: Biotechnology Authors: Chae JM, Heo W, Cho HT, Lee DH, Kim JH, Rhee MS, Park TS, Kim YK, Lee JH, Kim YJ Tags: J Microbiol Biotechnol Source Type: research

Secondary fermented extract of Chaga-Cheonggukjang attenuates the effects of obesity and suppresses inflammatory response in the liver and spleen of high-fat diet-induced obese mice.
Abstract Cheonggukjang and Chaga mushroom have numerous health benefits, and have been used in alternative medicine. The powder mixture of 98: Cheonggukjang and 2: Chaga extracts were fermented with Lactobacillus acidophilus KCTC3925 (FCC) and its obesity effects in high fat diet (HFD)-induced obese mice were determined. Five-week-old male ICR mice were fed a normal diet or HFD in the presence or absence of 3% and 5% FCC (FCC3 and FCC5) by weight (n = 10 per group). After 12 weeks, the mice were sacrificed, and serum and tissue samples were examined. Body weight and epididymal fat pad weight were significantly low...
Source: Journal of Microbiology and Biotechnology - April 27, 2019 Category: Biotechnology Authors: Na HG, Park Y, Kim MA, Lee JW, So G, Kim SH, Jang KH, Kim MJ, Namkoong S, Koo HJ, Lee SR, Sohn EH Tags: J Microbiol Biotechnol Source Type: research

Complete Genome Sequencing of Bacillus velezensis WRN014, and Comparison with Genome Sequences of other Bacillus velezensis strains.
This study reveals that the strain WRN014 and other Bacillus velezensis strains have potential to be used as plant growth-promoting rhizobacteria (PGPR) and biopesticide. PMID: 31030454 [PubMed - as supplied by publisher] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 27, 2019 Category: Biotechnology Authors: Wang J, Xing J, Lu J, Sun Y, Zhao J, Miao S, Xiong Q, Zhang Y, Zhang G Tags: J Microbiol Biotechnol Source Type: research

Increased tolerance to furfural by introduction of polyhydroxybutyrate synthetic genes to Escherichia coli.
In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB productio...
Source: Journal of Microbiology and Biotechnology - April 27, 2019 Category: Biotechnology Authors: Jung HR, Lee JH, Moon YM, Choi TR, Yang SY, Song HS, Park JY, Park YL, Bhatia SK, Gurav R, Ko BJ, Yang YH Tags: J Microbiol Biotechnol Source Type: research

Development of Molecular Marker through Genome Realignment for Specific Detection of Xanthomonas campestris pv. campestris race 5, the Pathogen of Black Rot Disease.
In this study, we developed a molecular marker specific to Xcc race 5. To do this, the available whole-genome sequence of Xcc races/strains and Xc subspecies were aligned and identified a highly variable genomic region (XccR5-89.2). Subsequently, a primer set covering the 'XccR5-89.2' region was designed and tested against the genomic DNA of Xcc races/strains, Xc subspecies and other plant infecting bacterial strains (Pseudomonas syringae pv. maculicola and Erwinia carotovora subsp. carotovora). The results showed that the 'XccR5-89.2' primer pair amplified 2172-bp fragment specific to Xcc race 5. Besides, they also amplif...
Source: Journal of Microbiology and Biotechnology - April 27, 2019 Category: Biotechnology Authors: Afrin KS, Rahim MA, Jung HJ, Park JI, Kim HT, Nou IS Tags: J Microbiol Biotechnol Source Type: research

Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber tree Ficus elastica Leaves.
In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi were evaluated against five pathogenic microorganisms. Two strains Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum) displayed broad-spectrum bioactivities. Our following study emphasized on the isolation, identification and bioactivity test of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3-5) were...
Source: Journal of Microbiology and Biotechnology - April 22, 2019 Category: Biotechnology Authors: Ding Z, Tao T, Wang L, Zhao Y, Huang H, Zhang D, Liu M, Wang Z, Han J Tags: J Microbiol Biotechnol Source Type: research

Cyanidin 3-O-glucoside Chloride Attenuates Streptococcus suis-Induced Inflammation by Inhibiting MAPK and NF- κB Signaling Pathways in Murine Macrophage J774 Cells.
Cyanidin 3-O-glucoside Chloride Attenuates Streptococcus suis-Induced Inflammation by Inhibiting MAPK and NF-κB Signaling Pathways in Murine Macrophage J774 Cells. J Microbiol Biotechnol. 2019 Apr 22;: Authors: Shen X, Li G, Deng X, Wang J, Zhang P Abstract Streptococcus suis serotype 2 (SS2), considered to be the most frequent and virulent type among identified serotypes, is an important emerging or re-emerging zoonotic pathogen. Inflammation is thought to be a hallmark of SS2 infection, as demonstrated by most clinical symptoms varying from an asymptomatic bacteremia to a fulminant systemic di...
Source: Journal of Microbiology and Biotechnology - April 22, 2019 Category: Biotechnology Authors: Shen X, Li G, Deng X, Wang J, Zhang P Tags: J Microbiol Biotechnol Source Type: research

Antidiabetic drugs and their nanoconjugates repurposed as novel antimicrobial agents against Acanthamoeba castellanii.
Abstract Acanthamoeba castellanii belonging to the T4 genotype may cause fatal brain infection known as granulomatous amoebic encephalitis, and vision-threatening eye infection Acanthamoeba keratitis. The aim of this study was to evaluate the antiamoebic effects of three clinically available antidiabetic drugs Glimepiride, Vildagliptin and Repaglinide against A. castellanii belonging to the T4 genotype. Furthermore, these drugs were attempted to conjugate with silver nanoparticles (AgNPs) to enhance antiamoebic effects. Amoebicidal, encystation, excystation, and host cells cytotoxicity assays were performed to unr...
Source: Journal of Microbiology and Biotechnology - April 22, 2019 Category: Biotechnology Authors: Anwar A, Siddiqui R, Raza Shah M, Khan NA Tags: J Microbiol Biotechnol Source Type: research

Microencapsulation of probiotic Lactobacillus acidophilus KBL409 by extrusion technology to enhance survival under simulated intestinal and freeze-drying conditions.
Abstract The probiotic Lactobacillus acidophilus KBL409 was encapsulated with alginate (Al) and alginate-chitosan (Al/Chi) through extrusion method. The sizes and zeta potentials of microspheres were measured to confirm encapsulation. To evaluate the protective effect of microspheres against gastrointestinal fluids, all the samples were exposed to simulated gastric fluids (SGFs, pH 1.5) at 37°C for 1 or 2 h, followed by incubation with simulated intestinal fluids (SIFs, pH 7.0) for 2 h. The mucoadhesive ability of microspheres was evaluated using the intestinal epithelial cell line HT29-MTX. To extend the shel...
Source: Journal of Microbiology and Biotechnology - April 22, 2019 Category: Biotechnology Authors: Lee Y, Ji YR, Lee S, Choi MJ, Cho Y Tags: J Microbiol Biotechnol Source Type: research

Soybean fermented with Bacillus amyloliquefaciens (Cheonggukjang) ameliorate atopic dermatitis-like skin lesion in mice by suppressing infiltration of mast cells and production of IL-31 cytokine in skin lesions.
Abstract The present study was conducted with the aim to investigate the ameliorative effects of new soybean product (cheonggukjang) fermented with Bacillus amyloliquefaciens SCGB1 (SFBA) in atopic dermatitis (AD) mouse model. Visual evaluation of AD induction in the mice indicated a remarkable control of SFBA in reducing pathological severity of AD-like skin lesions reported as the SCORAD score of AD clinical symptoms. The results revealed that SFBA reduced dorsal skin and epidermal thickness to a similar extent with prednisolone. Further analysis revealed the dominance of SFBA in restraining mast cell infiltrati...
Source: Journal of Microbiology and Biotechnology - April 12, 2019 Category: Biotechnology Authors: Cho BO, Shin JY, Kim JS, Che DN, Kang HJ, Jeong DY, Jang SI Tags: J Microbiol Biotechnol Source Type: research

Micronized and heat-treated Lactobacillus plantarum LM1004 stimulates host immune responses via the TLR-2/MAPK/NF- κB signalling pathway in vitro and in vivo.
This study aimed to identify the detailed biochemical mechanism of immune modulation by micronized and heat-treated L. plantarum LM1004 (MHT-LM1004,
Source: Journal of Microbiology and Biotechnology - April 12, 2019 Category: Biotechnology Authors: Lee J, Jung I, Choi JW, Lee CW, Cho S, Choi TG, Sohn M, Park YI Tags: J Microbiol Biotechnol Source Type: research