Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from Geobacillus thermoglucosidasius SKF4
J Microbiol Biotechnol. 2023 Nov 15;34(2):1-21. doi: 10.4014/jmb.2306.06050. Online ahead of print.ABSTRACTSeveral thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Suleiman D Allison Nur AdeelaYasid Fairolniza Mohd Shariff Nor'Aini Abdul Rahman Source Type: research

Protective Effects of Heat-Killed < em > Ruminococcus albus < /em > against β-AmyloidInduced Apoptosis on SH-SY5Y Cells
J Microbiol Biotechnol. 2023 Oct 31;34(1):1-9. doi: 10.4014/jmb.2308.08045. Online ahead of print.ABSTRACTA high level of β-amyloid (Aβ) in the brains of patients with Alzheimer's disease (AD) generates reactive oxygen species that induce neuronal death and DNA damage. The interaction between the gut microbiota and brain health has attracted attention in recent years. Heat-killed Ruminococcus albus (hkRA) reportedly protects neurons against damage induced by oxidative stress. However, whether hkRA can inhibit Aβ-induced apoptosis and thus alleviate AD remains unclear. Hence, we aimed to evaluate the protective effects o...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Seungmoon Choo Mirae An Young-Hee Lim Source Type: research

In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide
This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally, mRNA levels of bone metabolism-related factors such as the collagen type I alpha 1 chain, osteocalcin (OCN), osterix, bone sialoprotein, and the Runt family-associated transcription factor 2 were increased in cells treated ...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Jae Min Hwang Mun-Hoe Lee Yuri Kwon Hee-Chul Chung Do-Un Kim Jin-Hee Lee Source Type: research

LPS-Induced Modifications in Macrophage Transcript and Secretion Profiles Are Linked to Muscle Wasting and Glucose Intolerance
This study aimed to explore the regulation of macrophage-secreted factors and their impact on muscle atrophy and glucose metabolism. By employing RNA sequencing (RNA-seq) and proteome array, we uncovered that factors secreted by lipopolysaccharide (LPS)-stimulated macrophages upregulated markers of muscle atrophy and pro-inflammatory cytokines, while concurrently reducing glucose uptake in muscle cells. The RNA-seq analysis identified alterations in gene expression patterns associated with immune system pathways and nutrient metabolism. The utilization of gene ontology (GO) analysis and proteome array with macrophage-condi...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Heeyeon Ryu Hyeon Hak Jeong Seungjun Lee Min-Kyeong Lee Myeong-Jin Kim Bonggi Lee Source Type: research

Optimizing Conditions for the Production of Bacterial Extracellular Vesicles of < em > Vibrio vulnificus < /em > and Analysis of the Inner Small RNA Compositions
J Microbiol Biotechnol. 2023 Dec 5;34(2):1-10. doi: 10.4014/jmb.2310.10002. Online ahead of print.ABSTRACTChemical and physical elements affecting the production of bacterial extracellular vesicles (BEVs) of the human pathogen Vibrio vulnificus were quantitatively assessed to optimize the conditions for the BEV production by using the western blot quantification for an outer membrane porin OmpU and by fluorescent dye FM4-64. When cells were cultured at 37oC in an enriched medium (2Luria Bertani; 2LB) in the presence of EDTA, they produced about 70% more BEVs. BEVs were purified from the cells cultured in the establis...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: JeongHeon Park Suji Song Soyee Kim Minjeong Kim Kun-Soo Kim Source Type: research

Anthocyanins Inhibits Oxidative Injury in Human Retinal Pigment Epithelial ARPE-19 Cells < em > vi < /em > a Activating Heme Oxygenase-1
This study aimed to investigate whether anthocyanins could inhibit hydrogen peroxide (H2O2)-induced oxidative damage in human retinal pigment epithelial ARPE-19 cells. Our results indicated that anthocyanins suppressed H2O2-induced genotoxicity, while inhibiting reactive oxygen species (ROS) production and preserving diminished glutathione. Anthocyanins also suppressed H2O2-induced apoptosis by reversing the Bcl-2/Bax ratio and inhibiting caspase-3 activation. Additionally, anthocyanins attenuated the release of cytochrome c into the cytosol, which was achieved by interfering with mitochondrial membrane disruption. Moreove...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Cheol Park Hyun Hwangbo Sung Ok Kim Jeong Sook Noh Shin-Hyung Park Su Hyun Hong Sang Hoon Hong Gi-Young Kim Yung Hyun Choi Source Type: research

Production of Mono-Hydroxylated Derivatives of Terpinen-4-ol by Bacterial CYP102A1 Enzymes
In this study, we investigated the catalytic activity of engineered CYP102A1 on terpinen-4-ol. Among CYP102A1 mutants tested here, the R47L/F81I/F87V/E143G/L188Q/N213S/E267V mutant showed the highest activity to terpinen-4-ol. Two major metabolites of terpinen-4-ol were generated by engineered CYP102A1. Characterization of major metabolites was confirmed by liquid chromatography-mass spectrometry (LC-MS), gas chromatography-MS, and nuclear magnetic resonance spectroscopy (NMR). Based on the LC-MS results, the difference in mass-to-charge ratio of an ion (m/z) between terpinen-4-ol and its major metabolites was 16. One majo...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Jeong-Hoon Kim Chan Mi Park Hae Chan Jeong Gyeong Han Jeong Gun Su Cha Sungbeom Lee Chul-Ho Yun Source Type: research

Overproduction of < em > xanthophyll < /em > Pigment in < em > Flavobacterium < /em > sp. JSWR-1 under Optimized Culture Conditions
J Microbiol Biotechnol. 2023 Nov 24;34(3):1-15. doi: 10.4014/jmb.2310.10034. Online ahead of print.ABSTRACTFlavobacterium can synthesize xanthophyll, particularly the pigment zeaxanthin, which has significant economic value in nutrition and pharmaceuticals. Recently, the use of carotenoid biosynthesis by bacteria and yeast fermentation technology has shown to be very efficient and offers significant advantages in large-scale production, cost-effectiveness, and safety. In the present study, JSWR-1 strain capable of producing xanthophyll pigment was isolated from a freshwater reservoir in Wanju-gun, Republic of Korea. Based ...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Jegadeesh Raman Young-Joon Ko Jeong-Seon Kim Da-Hye Kim Soo-Jin Kim Source Type: research

Serotype-Dependent Inhibition of < em > Streptococcus pneumoniae < /em > Growth by Short-Chain Fatty Acids
In this study, we investigated the antimicrobial activity of SCFAs (acetate, propionate, and butyrate) against various serotypes pneumococci. Propionate generally inhibited the growth of S. pneumoniae serotypes included in the pneumococcal conjugate vaccine (PCV) 13, except for serotype 3 and 7F, though butyrate and acetate showed no or low inhibition, depending on the serotypes. Of note, butyrate showed strong inhibition against serotype 3, the most prevalent invasive strain since the introduction of the PCV vaccine. No SCFAs showed inhibitory effects against serotype 7F. Remarkably, the nonencapsulated pneumococcal strai...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Suwon Lim Dongwook Lee Sungho Jeong Jeong Woo Park Jintaek Im Bokeum Choi Donghyun Gwak Cheol-Heui Yun Ho Seong Seo Seung Hyun Han Source Type: research

Discovery of a Novel Cellobiose Dehydrogenase from < em > Cellulomonas palmilytica < /em > EW123 and Its Sugar Acids Production
This study establishes CpCDH as a novel and attractive bacterial CDH, representing the first report of its kind in the Cellulomonas genus.PMID:38044713 | DOI:10.4014/jmb.2307.07004 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Ake-Kavitch Siriatcharanon Sawannee Sutheeworapong Sirilak Baramee Rattiya Waeonukul Patthra Pason Akihiko Kosugi Ayaka Uke Khanok Ratanakhanokchai Chakrit Tachaapaikoon Source Type: research

Inhibitory Effects of < em > Latilactobacillus curvatus < /em > BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic Mice
In this study, the effect of BYB3, Lactobacillus rhamnosus GG, and Lactobacillus acidophilus GP1B strain extracts at various concentrations was examined on B16F10, a mouse melanoma cell line. Cell viability was examined via MTT assay, and the results indicated that compared to the other two probiotics, BYB3 significantly decreased the total percentages of viable cells. The effects of BYB3 on cell migration and proliferation in B16F10 cells were evaluated using wound healing mobility and proliferation assays, respectively; the results indicated that BYB3 inhibits cell migration and proliferation in a concentration-dependent...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Dingyun Li Xing Wang Dong-June Park Dong Hun Lee Sejong Oh Source Type: research

Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from Geobacillus thermoglucosidasius SKF4
J Microbiol Biotechnol. 2023 Nov 15;34(2):1-21. doi: 10.4014/jmb.2306.06050. Online ahead of print.ABSTRACTSeveral thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Suleiman D Allison Nur AdeelaYasid Fairolniza Mohd Shariff Nor'Aini Abdul Rahman Source Type: research

Protective Effects of Heat-Killed < em > Ruminococcus albus < /em > against β-AmyloidInduced Apoptosis on SH-SY5Y Cells
J Microbiol Biotechnol. 2023 Oct 31;34(1):1-9. doi: 10.4014/jmb.2308.08045. Online ahead of print.ABSTRACTA high level of β-amyloid (Aβ) in the brains of patients with Alzheimer's disease (AD) generates reactive oxygen species that induce neuronal death and DNA damage. The interaction between the gut microbiota and brain health has attracted attention in recent years. Heat-killed Ruminococcus albus (hkRA) reportedly protects neurons against damage induced by oxidative stress. However, whether hkRA can inhibit Aβ-induced apoptosis and thus alleviate AD remains unclear. Hence, we aimed to evaluate the protective effects o...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Seungmoon Choo Mirae An Young-Hee Lim Source Type: research

In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide
This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally, mRNA levels of bone metabolism-related factors such as the collagen type I alpha 1 chain, osteocalcin (OCN), osterix, bone sialoprotein, and the Runt family-associated transcription factor 2 were increased in cells treated ...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Jae Min Hwang Mun-Hoe Lee Yuri Kwon Hee-Chul Chung Do-Un Kim Jin-Hee Lee Source Type: research

LPS-Induced Modifications in Macrophage Transcript and Secretion Profiles Are Linked to Muscle Wasting and Glucose Intolerance
This study aimed to explore the regulation of macrophage-secreted factors and their impact on muscle atrophy and glucose metabolism. By employing RNA sequencing (RNA-seq) and proteome array, we uncovered that factors secreted by lipopolysaccharide (LPS)-stimulated macrophages upregulated markers of muscle atrophy and pro-inflammatory cytokines, while concurrently reducing glucose uptake in muscle cells. The RNA-seq analysis identified alterations in gene expression patterns associated with immune system pathways and nutrient metabolism. The utilization of gene ontology (GO) analysis and proteome array with macrophage-condi...
Source: Journal of Microbiology and Biotechnology - December 4, 2023 Category: Biotechnology Authors: Heeyeon Ryu Hyeon Hak Jeong Seungjun Lee Min-Kyeong Lee Myeong-Jin Kim Bonggi Lee Source Type: research