Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Intracellular Flux Prediction of Recombinant Escherichia coli Producing Gamma-aminobutyric Acid
This study utilized the GEM to observe the internal metabolic fluxes of recombinant Escherichia coli producing gamma-aminobutyric acid (GABA). Recombinant E. coli was cultivated in a fermenter under three conditions: pH 7, pH 5, and additional succinic acids. External fluxes were calculated from cultivation results, and internal fluxes were calculated through flux optimization. Based on the internal flux analysis, glycolysis and pentose phosphate pathways were repressed under cultivation at pH 5, even though glutamate dehydrogenase increased GABA production. Notably, this repression was halted by adding succinic acid. Furt...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Sung Han Bae Myung Sub Sim Ki Jun Jeong Dan He Inchan Kwon Tae Wan Kim Hyun Uk Kim Jong-Il Choi Source Type: research

Optimization of Ultrasound-Assisted Pretreatment for Accelerating Rehydration of Adzuki Bean ( Vigna angularis )
This study aimed to determine the optimal conditions of ultrasound treatment for the improved hydration of adzuki beans using the response surface methodology (RSM). Independent variables chosen to regulate the hydration process of the adzuki beans were the soaking time (2-14 h, X1), treatment intensity (150-750 W, X2), and treatment time (1- 10 min, X3). Dependent variables chosen to assess the differences in the beans post-immersion were moisture content, water activity, and hardness. The optimal conditions for treatment deduced through RSM were a soaking time of 12.9 h, treatment intensity of 600 W, and treatment time o...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Changgeun Lee Hyengseop Kim Eunghee Kim Youngje Jo Jiyoon Park Choongjin Ban Seokwon Lim Source Type: research

Advancements in Sustainable Plant-Based Alternatives: Exploring Proteins, Fats, and Manufacturing Challenges in Alternative Meat Production
J Microbiol Biotechnol. 2024 Feb 19;34(5):1-10. doi: 10.4014/jmb.2312.12049. Online ahead of print.ABSTRACTThe rise in plant-based food consumption is propelled by concerns for sustainability, personal beliefs, and a focus on healthy dietary habits. This trend, particularly in alternative meat, has attracted attention from specialized brands and eco-friendly food companies, leading to increased interest in plant-based alternatives. The dominant plant-based proteins, derived mainly from legumes, include soy protein isolates, which significantly impact sensory factors. In the realm of plant-based fats, substitutes are catego...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Minju Jung YouKyeong Lee Sung Ok Han Jeong Eun Hyeon Source Type: research

Unveiling immunomodulatory effects of Euglena gracilis in immunosuppressed mice: Transcriptome and pathway analysis
This study was designed not only to investigate the immunomodulatory effects but also to determine the genetic mechanisms of Euglena and β-glucan in cyclophosphamide (CCP)-induced immunosuppressed mice. The animals were orally administered saline, Euglena (800 mg/kg B.W.) or β-glucan (400 mg/kg B.W.) for 19 days, and CCP (80 mg/kg B.W.) was subsequently administered to induce immunosuppression in the mice. The mice exhibited significant decreases in body weight, organ weight, and the spleen index. However, there were significant improvements in the spleen weight and the spleen index in CCP-induced mice after the oral adm...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Seon Ha Jo Kyeong Ah Jo Soo-Yeon Park Ji Yeon Kim Source Type: research

Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery
J Microbiol Biotechnol. 2024 Feb 19;34(5):1-10. doi: 10.4014/jmb.2311.11024. Online ahead of print.ABSTRACTDespite considerable advancements achieved using next-generation sequencing technologies in exploring microbial diversity, several species of the gut microbiome remain unknown. In this transformative era, culturomics has risen to prominence as a pivotal approach in unveiling realms of microbial diversity that were previously deemed inaccessible. Utilizing innovative strategies to optimize growth and culture medium composition, scientists have successfully cultured hard-to-cultivate microbes. This progress has fostered...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Hye Seon Song Yeon Bee Kim Joon Yong Kim Seong Woon Roh Tae Woong Whon Source Type: research

1-kestose blocks UVB-induced skin inflammation and promotes Type I procollagen synthesis via regulating MAPK/AP-1, NF- κB and TGF-β/Smad pathway
J Microbiol Biotechnol. 2024 Feb 22;35(5):1-10. doi: 10.4014/jmb.2311.11020. Online ahead of print.ABSTRACTSolar UVB irradiation cause skin photoaging by inducing the high expression of matrix metalloproteinase (MMPs) to inhibit the expression of Type1 procollagen synthesis. 1-kestose, a natural trisaccharide, has been indicated to show a cytoprotective role in UVB radiation-induced-HaCaT cells. However, few studies have confirmed the anti-aging effects. In the present study, we evaluated the anti-photoaging and pathological mechanism of 1-kestose using Human keratinocytes (HaCaT) cells. The results found that 1-kestose pr...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Jihye Baek Jong-Hwa Kim Jiwon Park Do Hyun Kim Soonok Sa Jung-Sook Han Wonyong Kim Source Type: research

Improving the Three-Dimensional Printability of Potato Starch Loaded onto Food Ink
This study focuses on improving the 3D printability of pea protein with the help of food inks designed for jet-type 3D printers. Initially, the food ink base was formulated using nanocellulose-alginate with a gradient of native potato starch and its 3D printability was evaluated. The 3D-printed structures using only candidates for the food ink base formulated with or without potato starch exhibited dimensional accuracy exceeding 95% on both the X and Y axes. However, the accuracy of stacking on the Z-axis was significantly affected by the ink composition. Food ink with 1% potato starch closely matched the CAD design, with ...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Yourim Oh Seungmin Lee Nam Keun Lee Jin-Kyu Rhee Source Type: research

Establishment of a Dual-Vector System for Gene Delivery Utilizing Prototype Foamy Virus
This study introduces a novel platform technology using a pseudo-virus with single-round infectivity. In contrast to previous vector approaches, we developed a technique employing only two vectors, pcHFV lacking Env and pCMV-Env, to introduce the desired genes into target cells. Our investigation demonstrated the efficacy of the prototype foamy virus (PFV) dual-vector system in producing viruses and delivering transgenes into host cells. To optimize viral production, we incorporated the codon-optimized Env (optEnv) gene in pCMV-Env and the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) at the 3' en...
Source: Journal of Microbiology and Biotechnology - February 21, 2024 Category: Biotechnology Authors: Soo-Yeon Cho Yoon Jae Lee Seong-Mook Jung Young Min Son Cha-Gyun Shin Eui Tae Kim Kyoung-Dong Kim Source Type: research

Specific Alternation of Gut Microbiota and the Role of Ruminococcus gnavus in the Development of Diabetic Nephropathy
In this study, we aim to investigate the precise alterations in the gut microbiota during the onset and advancement of diabetic nephropathy (DN) and examine the impact of Ruminococcus gnavus (R. gnavus) on DN. Eight-week-old male KK-Ay mice were administered antibiotic cocktails for a duration of two weeks, followed by oral administration of R. gnavus for an additional eight weeks. Our study revealed significant changes in the gut microbiota during both the initiation and progression of DN. Specifically, we observed a notable increase in the abundance of Clostridia at the class level, higher levels of Lachnospirales and Os...
Source: Journal of Microbiology and Biotechnology - February 12, 2024 Category: Biotechnology Authors: Jinni Hong Tingting Fu Weizhen Liu Yu Du Junmin Bu Guojian Wei Miao Yu Yanshan Lin Cunyun Min Datao Lin Source Type: research

Understanding a Core Pilin of the Type IVa Pili of Acidithiobacillus thiooxidans , PilV
In this study we present a successful ad hoc methodology for the heterologous expression and purification of extracellular proteins such as the minor pilin PilV of the T4P of A. thiooxidans, a pilin exposed to extreme conditions of acidity and high oxidation-reduction potentials, and that interact with metal sulfides in an environment rich in dissolved minerals. Once obtained, the model structure of A. thiooxidans PilV revealed the core basic architecture of T4P pilins. Because of the acidophilic condition, we carried out in silico characterization of the protonation status of acidic and basic residues of PilV in order to ...
Source: Journal of Microbiology and Biotechnology - February 12, 2024 Category: Biotechnology Authors: Araceli Hern ández-Sánchez Edgar D P áez-Pérez Elvia Alfaro-Salda ña Vanesa Olivares-Illana J Viridiana Garc ía-Meza Source Type: research

Coffee Husk By-Product as Novel Ingredients for Cascara Kombucha Production
This study aims to investigate the potential suitability of cascara as a novel ingredient for kombucha production. Our findings suggested that cascara is a suitable substrate for kombucha production. Fermentation elevated the total phenolic and flavonoid content in cascara, which enhanced the antioxidant, antibacterial, and prebiotic characteristics of the product. Furthermore, the accumulation of acetic acid-induced the pH lowering reached 2.7 after 14 days of fermentation, which achieved the microbiological safety of the product. Moreover, 14 days of fermentation resulted in a balanced amalgamation of acidity, sweetness,...
Source: Journal of Microbiology and Biotechnology - February 12, 2024 Category: Biotechnology Authors: Bao Xuyen Nguyen Le Thach Phan Van Quang Khai Phan Gia Bao Pham Hoa Pham Quang Anh Duy Do Source Type: research

Synbiotic of Pediococcus acidilactici and Inulin Ameliorates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice
In this study, a synbiotic composed of inulin and Pediococcus acidilactici (P. acidilactici) was investigated for its ability to alleviate dextran sulfate sodium (DSS)-induced colitis. The results revealed that the synbiotic, composed of inulin and P. acidilactici, attenuated the body weight loss and disease activity index (DAI) score in mice with DSS-mediated colitis. Determination of biochemical indicators found that the synbiotic increased anti-oxidation and alleviated inflammation in mice. Additionally, histopathological examination revealed that colonic goblet cell loss and severe mucosal damage in the model group wer...
Source: Journal of Microbiology and Biotechnology - February 12, 2024 Category: Biotechnology Authors: Mingzhu Wang Longzhou Zhang Huiyan Piao Yuanming Jin Chengdu Cui Xin Jin Lianhua Cui Chunri Yan Source Type: research