Intracellular Flux Prediction of Recombinant < em > Escherichia coli < /em > Producing Gamma-aminobutyric Acid
This study utilized the GEM to observe the internal metabolic fluxes of recombinant Escherichia coli producing gamma-aminobutyric acid (GABA). Recombinant E. coli was cultivated in a fermenter under three conditions: pH 7, pH 5, and additional succinic acids. External fluxes were calculated from cultivation results, and internal fluxes were calculated through flux optimization. Based on the internal flux analysis, glycolysis and pentose phosphate pathways were repressed under cultivation at pH 5, even though glutamate dehydrogenase increased GABA production. Notably, this repression was halted by adding succinic acid. Furthermore, proper sucA repression is a promising target for developing strains more capable of producing GABA.PMID:38379308 | DOI:10.4014/jmb.2312.12022
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Sung Han Bae Myung Sub Sim Ki Jun Jeong Dan He Inchan Kwon Tae Wan Kim Hyun Uk Kim Jong-Il Choi Source Type: research