Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Diet-induced gut dysbiosis and leaky gut syndrome
This study highlights the significance of exploring the impact of diet on gut microbiota and the underlying mechanisms in the gut-liver and gut-brain axis. Nevertheless, limited research on the gut-liver axis poses challenges in comprehending the intricate connections between diet and the gut-brain axis. This underscores the need for comprehensive studies to elucidate the intricate gut-brain mechanisms underlying intestinal health and microbiota.PMID:38321650 | DOI:10.4014/jmb.2312.12031 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 7, 2024 Category: Biotechnology Authors: Yu-Rim Chae Yu Ra Lee Young-Soo Kim Ho-Young Park Source Type: research

 Lactobacillus Persisters Formation and Resuscitation
This study provides a method for forming and verifying the persistence of Lactobacillus and demonstrates that antibiotic-induced Lactobacillus persister cells show characteristics of dormancy, sensitivity of antibiotics, same as exponential cells, multi-drug tolerance, and resuscitation, which are characteristics of general persister cells. This study suggests that the mechanisms of formation and resuscitation may vary depending on the characteristics, such as the membrane structure of the bacterial species.PMID:38326923 | DOI:10.4014/jmb.2312.12035 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - February 7, 2024 Category: Biotechnology Authors: Hyein Kim Sejong Oh Sooyeon Song Source Type: research

In vitro anti-inflammatory and skin protective effects of Codium fragile extract on macrophages and human keratinocytes in atopic dermatitis
J Microbiol Biotechnol. 2024 Jan 17;34(4):1-10. doi: 10.4014/jmb.2312.12002. Online ahead of print.ABSTRACTCodium fragile has been traditionally used in oriental medicine to treat enterobiasis, dropsy, and dysuria, and it has been shown to possess many biological properties. Atopic dermatitis (AD) is one of the types of skin inflammation and barrier disruption, which leads to chronic inflammatory skin diseases. In the current investigation, the protective effects of C. fragile extract (CFE) on anti-inflammation and skin barrier improvement were investigated. In LPS-stimulated RAW 264.7 cells, nitric oxide generation and th...
Source: Journal of Microbiology and Biotechnology - February 5, 2024 Category: Biotechnology Authors: A-Yeong Jang JeongUn Choi Weerawan Rod-In Ki Young Choi Dae-Hee Lee Woo Jung Park Source Type: research

Secretory production of the Hericium erinaceus laccase from Saccharomyces cerevisiae 
This study isolated a novel laccase, named HeLac4c, from endophytic white-rot fungi Hericium erinaceus mushrooms. The cDNAs for this enzyme were 1569 bp in length and encoded a protein of 523 amino acids, including a 20 amino-acid signal peptide. Active extracellular production of glycosylated laccases from Saccharomyces cerevisiae was successfully achieved by selecting an optimal translational fusion partner. We observed that 5 and 10 mM Ca2+, Zn2+, and K+ increased laccase activity, whereas 5 mM Fe2+ and Al3+ inhibited laccase activity. The laccase activity was inhibited by the addition of low concentrations of sodium az...
Source: Journal of Microbiology and Biotechnology - February 5, 2024 Category: Biotechnology Authors: Jin Kang Thuat Van La Mi-Jin Kim Jung-Hoon Bae Bong Hyun Sung Seonghun Kim Jung-Hoon Sohn Source Type: research

Autolysis of Pseudomonas aeruginosa Quorum-Sensing Mutant Is Suppressed by Staphylococcus aureus through IronDependent Metabolism
J Microbiol Biotechnol. 2024 Feb 2;34(4):1-9. doi: 10.4014/jmb.2312.12028. Online ahead of print.ABSTRACTMicroorganisms usually coexist as a multifaceted polymicrobial community in the natural habitats and at mucosal sites of the human body. Two opportunistic human pathogens, Pseudomonas aeruginosa and Staphylococcus aureus commonly coexist in the bacterial infections for hospitalized and/or immunocompromised patients. Here, we observed that autolysis of the P. aeruginosa quorum-sensing (QS) mutant (lasRmvfR) was suppressed by the presence of the S. aureus cells in vitro. The QS mutant still displayed killing against S. au...
Source: Journal of Microbiology and Biotechnology - February 2, 2024 Category: Biotechnology Authors: Shin-Yae Choi In-Young Chung Hee-Won Bae You-Hee Cho Source Type: research

Production of ρ-Hydroxyacetophenone by Engineered Escherichia coli Heterologously Expressing 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase
J Microbiol Biotechnol. 2023 Dec 8;34(2):1-10. doi: 10.4014/jmb.2310.10019. Online ahead of print.ABSTRACTρ-Hydroxyacetophenone is an important and versatile compound that has been widely used in medicine, cosmetics, new materials, and other fields. At present, there are two ways to obtain ρhydroxyacetophenone. One is to extract it from plants, such as Artemisia capillaris Thunb and Cynanchum otophyllum Schneid, and the other is to synthesize it by using chemical methods. Of these two methods, the second is the main one, although it has problems, such as flammable and explosive reagents, difficult separation of by-produc...
Source: Journal of Microbiology and Biotechnology - February 2, 2024 Category: Biotechnology Authors: Wenmei Wu Xiwei Yuan Xin Gao Chaoyang Tan Shunxiang Li Dehong Xu Source Type: research

Nasal Immunization Using Chitosan Nanoparticles with Glycoprotein B of Murine Cytomegalovirus
In this study, we tested the ability of nasally administered chitosan nanoparticles loaded with glycoprotein B of murine cytomegalovirus to induce an immune response in an animal model. The choice of chitosan nanoparticle type was made by in vitro evaluation of sorption efficiency and antigen release. Three types of chitosan nanoparticles were prepared: crosslinked with tripolyphosphate, coated with hyaluronic acid, and in complex with polycaprolactone. The hydrodynamic size of the nanoparticles by dynamic light scattering, zeta potential, Fourier transform infrared spectroscopy, scanning electron microscopy, stability, lo...
Source: Journal of Microbiology and Biotechnology - February 2, 2024 Category: Biotechnology Authors: Marcela Slovakova Sylva Janovska Radek Sleha Vera Radochova Alexandra Hatala Nikola Mannova Radovan Metelka Ludovit Pudelka Pavel Bostik Source Type: research

Polyphenol Contents, Gas Chromatography-Mass Spectrometry (GC-MS) and Antibacterial Activity of Methanol Extract and Fractions of Sonneratia Caseolaris Fruits from Ben Tre Province in Vietnam
J Microbiol Biotechnol. 2024 Jan 28;34(1):94-102. doi: 10.4014/jmb.2304.04019. Epub 2023 Sep 30.ABSTRACTPlants contain a large number of phytochemical components, many of which are known as bioactive compounds and responsible for the expression of various pharmacological activities. The extract of Sonneratia caseolaris fruit collected in Vietnam was investigated for its total phenolic and total flavonoid contents using methanol solvent and different fractions of S. caseolaris fruits (hexane, ethyl acetate, n-butanol, and aqueous). GC-MS analysis was conducted to identify the bioactive chemical constituents occurring in the...
Source: Journal of Microbiology and Biotechnology - January 29, 2024 Category: Biotechnology Authors: Thien Khanh Tran Pham Thi Thu Ha Robert J Henry Dang Nguyen Thao Nguyen Phung Thi Tuyen Nguyen Thanh Liem Source Type: research

Steamed Ginseng Berry Powder Ameliorates Skeletal Muscle Atrophy via Myogenic Effects
J Microbiol Biotechnol. 2024 Jan 28;34(1):157-166. doi: 10.4014/jmb.2309.09017. Epub 2023 Nov 17.ABSTRACTSarcopenia is an age-related loss of muscle mass and function for which there is no approved pharmacological treatment. We tested direct efficacy by evaluating grip strength improvement in a sarcopenia mouse model rather than drug screening, which inhibits specific molecular mechanisms. Various physiological functions of ginseng berries are beneficial to the human body. The present study aimed to evaluate the efficacy and safety of steamed ginseng berry powder (SGBP). SGBP administration increased myotube diameter and s...
Source: Journal of Microbiology and Biotechnology - January 29, 2024 Category: Biotechnology Authors: Jungbum Kim Hui-Ji Choi Donghyuk Seo Sang-Ah Lee Jong Beom Heo Dong Hyuk Baek Wonhwa Lee Gyu Yong Song Source Type: research

Isolation of a Potential Probiotic Levilactobacillus brevis and Evaluation of Its Exopolysaccharide for Antioxidant and α-Glucosidase Inhibitory Activities
J Microbiol Biotechnol. 2024 Jan 28;34(1):167-175. doi: 10.4014/jmb.2304.04043. Epub 2023 Oct 13.ABSTRACTThe probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. Levilactobacillus brevis L010 was one of the most active strains across all the in vitro tests. The cell-free supernatant (50 g/l) of L. brevis L010 showed high levels of both α-glucosidase inhibitory activity (98.73 ± 1.32%) and 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity (32.29 ± 3.86%). The EPS isolate...
Source: Journal of Microbiology and Biotechnology - January 29, 2024 Category: Biotechnology Authors: Se-Young Kwun Jeong-Ah Yoon Ga-Yeon Kim Young-Woo Bae Eun-Hee Park Myoung-Dong Kim Source Type: research

Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6
J Microbiol Biotechnol. 2024 Jan 28;34(1):224-231. doi: 10.4014/jmb.2308.08017. Epub 2023 Nov 13.ABSTRACTThe proteins carried by the extracellular vesicles of Lactobacillus salivarius SNK-6 (LsEVs) were identified to provide a foundation for further explorations of the probiotic activities of L. salivarius SNK-6. LsEVs were isolated from the culture media of L. salivarius SNK-6 and morphological analysis was conducted by scanning electron microscopy. Subsequent transmission electron microscopy and nanoparticle tracking analysis were performed to assess the morphology and particle size of the LsEVs. In addition, the protein...
Source: Journal of Microbiology and Biotechnology - January 29, 2024 Category: Biotechnology Authors: Jiwen Huang Ayong Zhao Daqian He Xiao Wu Huaxiang Yan Lihui Zhu Source Type: research

A Narrative Review on the Advance of Probiotics to Metabiotics
This study showed that neurological disorders, such as depression, anxiety, autism spectrum disorder, Alzheimer's disease, and Parkinson's disease, can be treated and prevented according to the gut-brain axis theory by changing the intestinal microflora. In addition, various studies are being conducted on the immunomodulatory and anticancer effects of substances related to metabiotics of the microbiome. In particular, its efficacy is expected to be confirmed through human studies on various cancers. Therefore, developing various health functional effects of the next-generation probiotics such as metabiotics to prevent or t...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Hye Ji Jang Na-Kyoung Lee Hyun-Dong Paik Source Type: research

A Replication-Competent Retroviral Vector Expressing the HERV-W Envelope Glycoprotein is a Potential Tool for Cancer Gene Therapy
In this study, we observed syncytium formation upon transfection of HeLa and TE671 human cancer cells with plasmids containing the HERV-W 483 gene. To explore the potential of a semi-replication-competent retroviral (s-RCR) vector encoding HERV-W 483 for FMG-mediated cancer gene therapy, we developed two replication-defective retroviral vectors: a gag-pol vector encoding HERV-W 483 (MoMLV-HERV-W 483) and an env vector encoding VSV-G (pCLXSN-VSV-G-EGFP). When MoMLV-HERV-W 483 and pCLXSN-VSV-G-EGFP were co-transfected into HEK293T cells to produce the s-RCR vector, gradual syncytium formation was observed. However, the titer...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Byoung Kwon Kang Yong-Tae Jung Source Type: research

Taxonomic Variations of Bacterial and Fungal Communities Depending on Fermentation Temperature in Traditional Korean Fermented Soybean Food, Doenjang
This study helps our understanding of meju fermentation process at different fermentation temperatures and highlights different bacteria and fungi associated with specific fermentation periods which may influence the nutritional and organoleptic properties of the final fermented soybean foods doenjang.PMID:38247211 | DOI:10.4014/jmb.2312.12024 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Eunhye Jo Hyeyoung Lee Younshil Song Jaeho Cha Source Type: research

Oral administration of Alcohol-Tolerant Lactic Acid Bacteria Alleviates Blood Alcohol Levels and Ethanol-Induced Liver Damage in Rodents
This study aimed to evaluate the potential of lactic acid bacteria (LAB) to alleviate alcohol-induced effects and behavioral responses. The two LAB strains isolated from kimchi, Levilactobacillus brevis WiKim0168 and Leuconostoc mesenteroides WiKim0172, were selected for their ethanol tolerance and potential to alleviate hangover symptoms. Enzyme activity assays for alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) were conducted to evaluate the role of these bacteria in alcohol metabolism. Through in vitro and in vivo studies, these strains were assessed for their ability to reduce blood alcohol concentrat...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Misun Yun Hee Eun Jo Namhee Kim Hyo Kyeong Park Young Seo Jang Ga Hee Choi Ha Eun Jo Seo Jeong Hyun Ji Ye Mok Sang Min Park Hak-Jong Choi Source Type: research