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Oral administration of Alcohol-Tolerant Lactic Acid Bacteria Alleviates Blood Alcohol Levels and Ethanol-Induced Liver Damage in Rodents
This study aimed to evaluate the potential of lactic acid bacteria (LAB) to alleviate alcohol-induced effects and behavioral responses. The two LAB strains isolated from kimchi, Levilactobacillus brevis WiKim0168 and Leuconostoc mesenteroides WiKim0172, were selected for their ethanol tolerance and potential to alleviate hangover symptoms. Enzyme activity assays for alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) were conducted to evaluate the role of these bacteria in alcohol metabolism. Through in vitro and in vivo studies, these strains were assessed for their ability to reduce blood alcohol concentrat...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Misun Yun Hee Eun Jo Namhee Kim Hyo Kyeong Park Young Seo Jang Ga Hee Choi Ha Eun Jo Seo Jeong Hyun Ji Ye Mok Sang Min Park Hak-Jong Choi Source Type: research

Hepatoprotective Effect of Lactiplantibacillus plantarum DSR330 in Mice with High Fat Diet-Induced Nonalcoholic Fatty Liver Disease
In this study, the hepatoprotective effects of DSR330 were examined against non-alcoholic fatty liver disease (NAFLD) in a high-fat diet (HFD)-fed C57BL/6 mouse model. To induce the development of fatty liver, a HFD was administered for five weeks, and then silymarin (positive control) or DSR330 (108 or 109 CFU/day) was administered along with the HFD for seven weeks. DSR330 significantly decreased body weight and altered serum and hepatic lipid profiles, including a reduction in triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels compared to those in the HFD group. DSR330 significantly alleviat...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Na-Kyoung Lee Yunjung Lee Da-Soul Shin Jehyeon Ra Yong-Min Choi Byung Hee Ryu Jinhyeuk Lee Eunju Park Hyun-Dong Paik Source Type: research

Gromwell ( Lithospermum erythrorhizon ) Attenuates High-Fat-Induced Skeletal Muscle Wasting by Increasing Protein Synthesis and Mitochondrial Biogenesis
J Microbiol Biotechnol. 2023 Dec 30;34(3):1-10. doi: 10.4014/jmb.2311.11034. Online ahead of print.ABSTRACTGromwell (Lithospermum erythrorhizon, LE) can mitigate obesity-induced skeletal muscle atrophy in C2C12 myotubes and high-fat diet (HFD)-induced obese mice. The purpose of this study was to investigate the anti-skeletal muscle atrophy effects of LE and the underlying molecular mechanism. C2C12 myotubes were pretreated with LE or Shikonin, and active component of LE, for 24 h and then treated with 500 µM palmitic acid (PA) for an additional 24 h. Additionally, mice were fed a HFD for 8 weeks to induced obesity, and th...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Ji-Sun Kim Hyunjung Lee Ahyoung Yoo Hang Yeon Jeong Chang Hwa Jung Jiyun Ahn Tae-Youl Ha Source Type: research

Ozonated Sunflower Oil (OSO) alleviates inflammatory responses in oxazolone-induced atopic dermatitis (AD)-like mice and LPS- treated RAW 264.7 cells
J Microbiol Biotechnol. 2024 Jan 12;34(4):1-10. doi: 10.4014/jmb.2310.10037. Online ahead of print.ABSTRACTOzone, a highly reactive oxidant molecule, is widely used as a complementary therapy for various skin diseases, including wound healing, pressure ulcers, diabetic foot, and infections. However, there is limited research on the effectiveness of ozone for atopic dermatitis (AD). Ozonated sunflower oil (OSO) is an active ingredient obtained from partially ozonated sunflower oil (SO). OSO markedly reduced the LPS-induced increase in IL-1β and nitric oxide (NO) levels in RAW 264.7 mouse macrophage cells. Oxazolone (OXZ) w...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Su-Young Kim Jung Ok Lee Sue Lee Jihye Heo Kyung-Hyun Cho Ashutosh Bahuguna Kwang-Ho Yoo Beom Joon Kim Source Type: research

Molecular and Phenotypic Investigation on Antibacterial Activities of Limonene Isomers and Its Oxidation Derivative against Xanthomonas oryzae pv. Oryzae 
J Microbiol Biotechnol. 2024 Jan 17;34(3):1-10. doi: 10.4014/jmb.2311.11016. Online ahead of print.ABSTRACTXanthomonas oryzae pv. oryzae (Xoo) causes a devastating bacterial leaf blight in rice. Here, the antimicrobial effects of D-limonene, L-limonene, and its oxidative derivative carveol against Xoo were investigated. We revealed that carveol treatment at ≥ 0.1 mM in liquid culture resulted in significant decrease in Xoo growth rate (> 40%) in a concentration-dependent manner, and over 1 mM, no growth was observed. The treatment with D-limonene and L-limonene also inhibited the Xoo growth but to a lesser extent comp...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Hyeonbin Kim Mi Hee Kim Ui-Lim Choi Moonsoo Chung Chul-Ho Yun Youngkun Shim Jaejun Oh Sungbeom Lee Gun Woong Lee Source Type: research

Inhibition of Polyphosphate Degradation in Synechocystis sp. PCC6803 through Inactivation of the phoU Gene
J Microbiol Biotechnol. 2024 Jan 8;34(2):1-8. doi: 10.4014/jmb.2311.11046. Online ahead of print.ABSTRACTPhosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis sp. with inhibited polyphosphate degradation and the effect of this engineered Synechocystis biomass on lettuce growth. ...
Source: Journal of Microbiology and Biotechnology - January 22, 2024 Category: Biotechnology Authors: Han-Bin Ryu Mi-Jin Kang Kyung-Min Choi Il-Kyu Yang Seong-Joo Hong Choul-Gyun Lee Source Type: research

Nodulation Experiment by Cross-Inoculation of Nitrogen-Fixing Bacteria Isolated from Root Nodules of Several Leguminous Plants
This study isolated the endophytic root-nodule bacteria from the nodules of legumes and examined them to determine whether they could be used to promote the formation of nodules in other legumes. Forty-six isolates were collected from five leguminous plants and screened for housekeeping (16S rRNA), nitrogen fixation (nifH), and nodulation (nodC) genes. Based on the 16S rRNA gene sequencing and phylogenetic analysis, the bacterial isolates WC15, WC16, WC24, and GM5 were identified as Rhizobium, Sphingomonas, Methylobacterium, and Bradyrhizobium, respectively. The four isolates were found to have the nifH gene, and the study...
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Ahyeon Cho Alpana Joshi Hor-Gil Hur Ji-Hoon Lee Source Type: research

Fisetin-Mediated Perturbations of Membrane Permeability and Intracellular pH in Candida albicans 
J Microbiol Biotechnol. 2024 Jan 10;34(4):1-10. doi: 10.4014/jmb.2311.11027. Online ahead of print.ABSTRACTThe antifungal activity of fisetin against Candida albicans is explored, elucidating a mechanism centered on membrane permeabilization and ensuing disruption of pH homeostasis. The Minimum Inhibitory Concentration (MIC) of fisetin, indicative of its interaction with the fungal membrane, increases in the presence of ergosterol. Hoechst 33342 and propidium-iodide staining reveal substantial propidium-iodide accumulation in fisetin-treated C. albicans cells at their MIC, with crystal violet uptake assays confirming fiset...
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Younhee Kim Source Type: research

Anti-Inflammatory Activity of Vacuum Distillate from Panax ginseng Root on LPS-Induced RAW264.7 Cells
J Microbiol Biotechnol. 2024 Jan 13;34(4):1-10. doi: 10.4014/jmb.2312.12001. Online ahead of print.ABSTRACTPanax ginseng has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of Panax ginseng distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on lipopolysaccharide-induced macrophages was assessed. The results showed that GSD effectively inhibited nitric oxide (NO) for...
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Chanwoo Lee Seul Lee Young Pyo Jang Junseong Park Source Type: research

Innate Immune-Enhancing Effect of Pinus densiflora Pollen Extract via NF- κB Pathway Activation
J Microbiol Biotechnol. 2023 Nov 29;34(4):1-10. doi: 10.4014/jmb.2309.09026. Online ahead of print.ABSTRACTConsidering the emergence of various infectious diseases, including the coronavirus disease 2019 (COVID-19), people's attention has shifted towards immune health. Consequently, immune-enhancing functional foods have been increasingly consumed. Hence, developing new immune-enhancing functional food products is needed. Pinus densiflora pollen can be collected from the male red pine tree, which is commonly found in Korea. P. densiflora pollen extract (PDE), obtained by water extraction, contained polyphenols (216.29 ± 0...
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Sehyeon Jang San Kim Se Jeong Kim Jun Young Kim Da Hye Gu Bo Ram So Jung A Ryu Jeong Min Park Sung Ran Yoon Sung Keun Jung Source Type: research

Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters
This study presents an effective strategy for enhancing indigo production using a combination of transporters.PMID:38213292 | DOI:10.4014/jmb.2308.08039 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Hyun Jin Kim Sion Ham Nara Shin Jeong Hyeon Hwang Suk Jin Oh Tae-Rim Choi Jeong Chan Joo Shashi Kant Bhatia Yung-Hun Yang Source Type: research

Enzymatic Activity and Amino Acids Production of Predominant Fungi from Traditional Meju During Soybean Fermentation
This study showed the potential of traditional meju strains as starters for soybean fermentation. However, further analysis of processes such as the production of G-peptide for kokumi taste and volatile compounds for flavor and safety is needed.PMID:38213301 | DOI:10.4014/jmb.2309.09008 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - January 12, 2024 Category: Biotechnology Authors: Dong Hyun Kim Byung Hee Chun Jae-Jung Lee Oh Cheol Kim Jiye Hyun Dong Min Han Che Ok Jeon Sang Hun Lee Sang-Han Lee Yong-Ho Choi Seung-Beom Hong Source Type: research

Structural and Biochemical Analysis of 3-Dehydroquinate Dehydratase from Corynebacterium glutamicum 
J Microbiol Biotechnol. 2023 Dec 28;33(12):1595-1605. doi: 10.4014/jmb.2305.05018. Epub 2023 Aug 18.ABSTRACTDehydroquinate dehydratase (DHQD) catalyzes the conversion of 3-dehydroquinic acid (DHQ) into 3-dehydroshikimic acid in the mid stage of the shikimate pathway, which is essential for the biosynthesis of aromatic amino acids and folates. Here, we report two the crystal structures of type II DHQD (CgDHQD) derived from Corynebacterium glutamicum, which is a widely used industrial platform organism. We determined the structures for CgDHQDWT with the citrate at a resolution of 1.80Å and CgDHQDR19A with DHQ complexed form...
Source: Journal of Microbiology and Biotechnology - December 28, 2023 Category: Biotechnology Authors: Chan Hwi Lee Sangwoo Kim Hogyun Seo Kyung-Jin Kim Source Type: research