Anti-Inflammatory Activity of Vacuum Distillate from < em > Panax ginseng < /em > Root on LPS-Induced RAW264.7 Cells

J Microbiol Biotechnol. 2024 Jan 13;34(4):1-10. doi: 10.4014/jmb.2312.12001. Online ahead of print.ABSTRACTPanax ginseng has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of Panax ginseng distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on lipopolysaccharide-induced macrophages was assessed. The results showed that GSD effectively inhibited nitric oxide (NO) formation and activation of inducible nitric oxide synthase (iNOS) mRNA in murine macrophage cell, but not cyclooxy-genase-2 production. The mRNA expression pattern of tumor necrosis factor alpha and IL-6 were also reduced by GSD. Furthermore, we confirmed that GSD exerted its anti-inflammatory effects by downregulating c-Jun NH2-terminal kinase (JNK) phosphorylation, the extracellular signal-regulated kinase phosphorylation, and signaling pathway of nuclear factor kappa B (NF-κB). Our findings revealed that the inflammatory regulation activity of GSD could be induced by iNOS and NO formation inhibition mediated by regulation of nuclear factor kappa B and p38/JNK MAPK pathways.PMID:38213284 | DOI:10.4014/jmb.2312.12001
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Source Type: research