Bioactive C-phycocyanin exerts immunomodulatory and antitumor activity in mice with induced melanoma
Toxicol Appl Pharmacol. 2024 Feb 28:116874. doi: 10.1016/j.taap.2024.116874. Online ahead of print.ABSTRACTMelanoma is the most aggressive and deadly skin cancer. The difficulty in its treatment arises from its ability to suppress the immune system, making it crucial to find a substance that increases anti-tumor immunity. C-phycocyanin (C-PC) appears as a promising bioactive, with multifaceted effects against several cancers, but its efficacy against melanoma has only been tested in vitro. Therefore, we investigated C-PC's the anti-tumor and immunomodulatory action in a murine melanoma model. The tumor was subcutaneously i...
Source: Toxicology and Applied Pharmacology - March 1, 2024 Category: Toxicology Authors: Mariana Teixeira Santos Figueiredo Salgado Mayara Cristini Sebasti ão Silva Camilly Fratelli Anna Rafaela Cavalcante Braga Thacyana Beatriz Guimar ães Lopes Enio Ferreira Istefani Luciene Dayse da Silva Luciana Souza de Paiva Ana Paula de Souza Votto Source Type: research

Tetramethylpyrazine inhibits the inflammatory response by downregulating the TNFR1/I κB-α/NF-κB p65 pathway after spinal cord injury
In conclusion, TMP inhibits inflammatory response and oxidative stress, thereby exerting a neuroprotective effect that may be related to the regulation of the TNFR1/IκB-α/NF-κB p65 signaling pathway.PMID:38428465 | DOI:10.1016/j.taap.2024.116872 (Source: Toxicology and Applied Pharmacology)
Source: Toxicology and Applied Pharmacology - March 1, 2024 Category: Toxicology Authors: Gang Liu Luyao Huo Bowen Deng Shengyuan Jiang Yi Zhao Yanjun Mo Huizhong Bai Lin Xu Chuanyu Hu Xiaohong Mu Source Type: research

Elucidating the distinctive regulatory effects and mechanisms of active compounds in Salvia miltiorrhiza Bunge via network pharmacology: Unveiling their roles in the modulation of platelet activation and thrombus formation
This study aimed to find out the potential targets and mechanisms of DS in the modulation of platelet activation and thrombosis, using network pharmacology and biological experimental. These compounds with anti-thrombotic activity in DS, cryptotanshinone (CPT), isoeugenol (ISO) and tanshinone IIA (TSA), together with the corresponding targets being Src, Akt and RhoA are screened by network pharmacology. We confirmed that ISO, CPT and TSA dose-dependently inhibited platelet activation in vitro, mainly by inhibiting agonist-induced clot retraction, aggregation and P-selectin and ATP release. The western blot findings indicat...
Source: Toxicology and Applied Pharmacology - February 29, 2024 Category: Toxicology Authors: Ying Zhang Guang Xin Qilong Zhou Xiuxian Yu Lijuan Feng Ao Wen Kun Zhang Tingyu Wen Xiaoli Zhou Qiuling Wu Hongchen He Wen Huang Source Type: research

Elucidating the distinctive regulatory effects and mechanisms of active compounds in Salvia miltiorrhiza Bunge via network pharmacology: Unveiling their roles in the modulation of platelet activation and thrombus formation
This study aimed to find out the potential targets and mechanisms of DS in the modulation of platelet activation and thrombosis, using network pharmacology and biological experimental. These compounds with anti-thrombotic activity in DS, cryptotanshinone (CPT), isoeugenol (ISO) and tanshinone IIA (TSA), together with the corresponding targets being Src, Akt and RhoA are screened by network pharmacology. We confirmed that ISO, CPT and TSA dose-dependently inhibited platelet activation in vitro, mainly by inhibiting agonist-induced clot retraction, aggregation and P-selectin and ATP release. The western blot findings indicat...
Source: Toxicology and Applied Pharmacology - February 29, 2024 Category: Toxicology Authors: Ying Zhang Guang Xin Qilong Zhou Xiuxian Yu Lijuan Feng Ao Wen Kun Zhang Tingyu Wen Xiaoli Zhou Qiuling Wu Hongchen He Wen Huang Source Type: research

Maternal exposure to glyphosate-based herbicide causes vascular dysfunction in offspring female rats
This study analyzed how glyphosate exposure in the gestational period affects vascular function in their female offspring and whether oxidative stress is involved in this effect. To this, pregnant Wistar rats were exposed through drinking water to 0.2% of a glyphosate commercial formulation, and we analyzed the response to acetylcholine and phenylephrine in the aorta from offspring of Glyphosate-based herbicide (O-GBH) and controls (O-CON) rats at six months of age. Relaxation to acetylcholine was reduced in O-GBH than in O-CON. Acute Indomethacin and Apocynin increased relaxation to acetylcholine in O-GBH. The aorta from ...
Source: Toxicology and Applied Pharmacology - February 28, 2024 Category: Toxicology Authors: Jeniffer Ailane Nunes Dayanne Cintia Alves de Lima Vivian Giselly Da Silva Moraes Marcos Ver íssimo de Oliveira Cardoso Luciano Augusto de Ara újo Ribeiro Diego Barbosa de Queiroz Fabr ício Souza Silva Source Type: research

Efficacy of a combined anti-seizure treatment against cholinergic established status epilepticus following a sarin nerve agent insult in rats
Toxicol Appl Pharmacol. 2024 Feb 21:116870. doi: 10.1016/j.taap.2024.116870. Online ahead of print.ABSTRACTThe development of refractory status epilepticus (SE) following sarin intoxication presents a therapeutic challenge. Here, we evaluated the efficacy of delayed combined double or triple treatment in reducing abnormal epileptiform seizure activity (ESA) and the ensuing long-term neuronal insult. SE was induced in rats by exposure to 1.2 LD50 sarin followed by treatment with atropine and TMB4 (TA) 1 min later. Double treatment with ketamine and midazolam or triple treatment with ketamine, midazolam and levetiracetam was...
Source: Toxicology and Applied Pharmacology - February 23, 2024 Category: Toxicology Authors: Lazar Shlomi Neufeld-Cohen Adi Egoz Inbal Baranes Shlomi Gez Rellie Glick Pnina Cohen Maayan Gutman Hila Chapman Shira Gore Ariel Source Type: research

Efficacy of a combined anti-seizure treatment against cholinergic established status epilepticus following a sarin nerve agent insult in rats
Toxicol Appl Pharmacol. 2024 Feb 21:116870. doi: 10.1016/j.taap.2024.116870. Online ahead of print.ABSTRACTThe development of refractory status epilepticus (SE) following sarin intoxication presents a therapeutic challenge. Here, we evaluated the efficacy of delayed combined double or triple treatment in reducing abnormal epileptiform seizure activity (ESA) and the ensuing long-term neuronal insult. SE was induced in rats by exposure to 1.2 LD50 sarin followed by treatment with atropine and TMB4 (TA) 1 min later. Double treatment with ketamine and midazolam or triple treatment with ketamine, midazolam and levetiracetam was...
Source: Toxicology and Applied Pharmacology - February 23, 2024 Category: Toxicology Authors: Lazar Shlomi Neufeld-Cohen Adi Egoz Inbal Baranes Shlomi Gez Rellie Glick Pnina Cohen Maayan Gutman Hila Chapman Shira Gore Ariel Source Type: research

Efficacy of a combined anti-seizure treatment against cholinergic established status epilepticus following a sarin nerve agent insult in rats
Toxicol Appl Pharmacol. 2024 Feb 21:116870. doi: 10.1016/j.taap.2024.116870. Online ahead of print.ABSTRACTThe development of refractory status epilepticus (SE) following sarin intoxication presents a therapeutic challenge. Here, we evaluated the efficacy of delayed combined double or triple treatment in reducing abnormal epileptiform seizure activity (ESA) and the ensuing long-term neuronal insult. SE was induced in rats by exposure to 1.2 LD50 sarin followed by treatment with atropine and TMB4 (TA) 1 min later. Double treatment with ketamine and midazolam or triple treatment with ketamine, midazolam and levetiracetam was...
Source: Toxicology and Applied Pharmacology - February 23, 2024 Category: Toxicology Authors: Lazar Shlomi Neufeld-Cohen Adi Egoz Inbal Baranes Shlomi Gez Rellie Glick Pnina Cohen Maayan Gutman Hila Chapman Shira Gore Ariel Source Type: research

Efficacy of a combined anti-seizure treatment against cholinergic established status epilepticus following a sarin nerve agent insult in rats
Toxicol Appl Pharmacol. 2024 Feb 21:116870. doi: 10.1016/j.taap.2024.116870. Online ahead of print.ABSTRACTThe development of refractory status epilepticus (SE) following sarin intoxication presents a therapeutic challenge. Here, we evaluated the efficacy of delayed combined double or triple treatment in reducing abnormal epileptiform seizure activity (ESA) and the ensuing long-term neuronal insult. SE was induced in rats by exposure to 1.2 LD50 sarin followed by treatment with atropine and TMB4 (TA) 1 min later. Double treatment with ketamine and midazolam or triple treatment with ketamine, midazolam and levetiracetam was...
Source: Toxicology and Applied Pharmacology - February 23, 2024 Category: Toxicology Authors: Lazar Shlomi Neufeld-Cohen Adi Egoz Inbal Baranes Shlomi Gez Rellie Glick Pnina Cohen Maayan Gutman Hila Chapman Shira Gore Ariel Source Type: research

Efficacy of a combined anti-seizure treatment against cholinergic established status epilepticus following a sarin nerve agent insult in rats
Toxicol Appl Pharmacol. 2024 Feb 21:116870. doi: 10.1016/j.taap.2024.116870. Online ahead of print.ABSTRACTThe development of refractory status epilepticus (SE) following sarin intoxication presents a therapeutic challenge. Here, we evaluated the efficacy of delayed combined double or triple treatment in reducing abnormal epileptiform seizure activity (ESA) and the ensuing long-term neuronal insult. SE was induced in rats by exposure to 1.2 LD50 sarin followed by treatment with atropine and TMB4 (TA) 1 min later. Double treatment with ketamine and midazolam or triple treatment with ketamine, midazolam and levetiracetam was...
Source: Toxicology and Applied Pharmacology - February 23, 2024 Category: Toxicology Authors: Lazar Shlomi Neufeld-Cohen Adi Egoz Inbal Baranes Shlomi Gez Rellie Glick Pnina Cohen Maayan Gutman Hila Chapman Shira Gore Ariel Source Type: research

Branching morphogenesis of the mouse mammary gland after exposure to benzophenone-3
In conclusion, exposure to BP3 does not impair mammary branching morphogenesis in our models. However, BP3 affects PR transcriptional expression and its downstream mediators, suggesting that exposure to BP3 might affect other developmental stages of the mammary gland.PMID:38382712 | DOI:10.1016/j.taap.2024.116868 (Source: Toxicology and Applied Pharmacology)
Source: Toxicology and Applied Pharmacology - February 21, 2024 Category: Toxicology Authors: Gonzalo Schierano-Marotti Gabriela A Altamirano Sofia Oddi Ayelen L Gomez Nicole Meyer M ónica Muñoz-de-Toro Ana C Zenclussen Horacio A Rodr íguez Laura Kass Source Type: research

Fenpropathrin provoked kidney damage via controlling the NLRP3/Caspase-1/GSDMD-mediated pyroptosis: The palliative role of curcumin-loaded chitosan nanoparticles
This study assessed the ability of formulated curcumin-loaded chitosan nanoparticles (CU-CS-NPs) to reduce the kidney damage resulting from fenpropathrin (FPN) in rats compared to curcumin (CU) in rats. Sixty male Sprague Dawley rats were separated into six groups and orally administered 1 mL/kg b.wt corn oil, 50 mg CU/kg b.wt, 50 mg CU-CS-NPs /kg b.wt., 15 mg FPN /kg b.wt, CU+ FPN or CU-CS-NPs + FPN for 60 days. Then, serum renal damage products were assessed. Total antioxidant capacity, reactive oxygen species, interleukin 1β (IL-1β), malondialdehyde, NF-κB P65, cleaved-Caspase-1, and Caspase-8 were estimated in kidne...
Source: Toxicology and Applied Pharmacology - February 21, 2024 Category: Toxicology Authors: Yasmina M Abd-Elhakim Amany Abdel-Rahman Mohamed Ahmed E Noreldin Tarek Khamis Areej A Eskandrani Ghalia Shamlan Wafa S Alansari Badriyah S Alotaibi Manal Alosaimi Mohammed Ageeli Hakami Samah S Abuzahrah Source Type: research

Branching morphogenesis of the mouse mammary gland after exposure to benzophenone-3
In conclusion, exposure to BP3 does not impair mammary branching morphogenesis in our models. However, BP3 affects PR transcriptional expression and its downstream mediators, suggesting that exposure to BP3 might affect other developmental stages of the mammary gland.PMID:38382712 | DOI:10.1016/j.taap.2024.116868 (Source: Toxicology and Applied Pharmacology)
Source: Toxicology and Applied Pharmacology - February 21, 2024 Category: Toxicology Authors: Gonzalo Schierano-Marotti Gabriela A Altamirano Sofia Oddi Ayelen L Gomez Nicole Meyer M ónica Muñoz-de-Toro Ana C Zenclussen Horacio A Rodr íguez Laura Kass Source Type: research

Fenpropathrin provoked kidney damage via controlling the NLRP3/Caspase-1/GSDMD-mediated pyroptosis: The palliative role of curcumin-loaded chitosan nanoparticles
This study assessed the ability of formulated curcumin-loaded chitosan nanoparticles (CU-CS-NPs) to reduce the kidney damage resulting from fenpropathrin (FPN) in rats compared to curcumin (CU) in rats. Sixty male Sprague Dawley rats were separated into six groups and orally administered 1 mL/kg b.wt corn oil, 50 mg CU/kg b.wt, 50 mg CU-CS-NPs /kg b.wt., 15 mg FPN /kg b.wt, CU+ FPN or CU-CS-NPs + FPN for 60 days. Then, serum renal damage products were assessed. Total antioxidant capacity, reactive oxygen species, interleukin 1β (IL-1β), malondialdehyde, NF-κB P65, cleaved-Caspase-1, and Caspase-8 were estimated in kidne...
Source: Toxicology and Applied Pharmacology - February 21, 2024 Category: Toxicology Authors: Yasmina M Abd-Elhakim Amany Abdel-Rahman Mohamed Ahmed E Noreldin Tarek Khamis Areej A Eskandrani Ghalia Shamlan Wafa S Alansari Badriyah S Alotaibi Manal Alosaimi Mohammed Ageeli Hakami Samah S Abuzahrah Source Type: research

Can exposure to lisdexamfetamine dimesylate from juvenile period to peripubertal compromise male reproductive parameters in adult rats?
This study evaluated the effects of exposure to LDX from the juvenile to peripubertal period (critical stages of development) on systemic and reproductive toxicity parameters in male rats. Male Wistar rats (23 days old) were treated with 0; 5.2; 8.6 or 12.1 mg/kg/day of LDX from post-natal day (PND) 23 to 53, by gavage. LDX treatment led to reduced daily food and water consumption, as well as a decrease in social behaviors. The day of preputial separation remained unaltered, although the treated animals exhibited reduced weight. At PND 54, the treated animals presented signs of systemic toxicity, evidenced by a reduction i...
Source: Toxicology and Applied Pharmacology - February 20, 2024 Category: Toxicology Authors: Julia Stein B árbara Campos Jorge L ívia Trippe Nagaoka Ana Carolina Casali Reis Beatriz de Matos Manoel Alana Rezende Godoi Vanessa Caroline Fioravante Francisco Eduardo Martinez Patr ícia Fernanda Felipe Pinheiro Andr é Sampaio Pupo Arielle Cristina Source Type: research