Maternal and infant MTHFR gene polymorphisms and non-syndromic oral cleft susceptibility: An updated meta-analysis
In conclusion, we determined that there is a strong association between the MTHFR C677 T polymorphism and NSCLP.Graphical Abstract (Source: Process Biochemistry)
Source: Process Biochemistry - October 20, 2019 Category: Biochemistry Source Type: research

Antiangiogenic, anti-inflammatory and their antioxidant activities of Turnera subulata Sm. (Turneraceae)
Publication date: Available online 18 October 2019Source: Process BiochemistryAuthor(s): Mythili Saravanan, Palanisamy Senthilkumar, Vajjiram Chinnadurai, Kunnathur Murugasan Sakthivel, Raman Rajeshkumar, Arivalagan PugazhendhiAbstractTurnera subulata is a substantial medicinal plant used in folk medicine to treat various ailments. The current study was to assess the total phenolic and flavonoid contents to evaluate the antioxidant and anti-inflammatory activities of the sequentially extracted T. subulata plant samples. In vitro anti-angiogenic activity was evaluated by chick chorioallantoic membrane (CAM) model for chloro...
Source: Process Biochemistry - October 20, 2019 Category: Biochemistry Source Type: research

A whole-cell process for the production of ε-caprolactone in aqueous media
We report on a whole-cell biocatalytic conversion of cyclohexanol to ε-caprolactone using the combination of an alcohol dehydrogenase (ADH) with two stability-improved variants (QM and M15) of the Baeyer-Villiger monooxygenase CHMO with special focus on process development at the 200 mM scale. Influence of parameters such as volumetric mass transfer co-efficient, stirrer speed and catalytic loading (amount of E. coli whole-cells expressing ADH and CHMO) on the process efficiency were studied and optimised. This resulted in over 98% conversion, a product titer of 20 g L–1 and an isolated product ...
Source: Process Biochemistry - October 19, 2019 Category: Biochemistry Source Type: research

FDS, a novel saponin isolated from Felicium decipiens: Lectin interaction and Biological complementary activities
Publication date: Available online 16 October 2019Source: Process BiochemistryAuthor(s): Romero M.P. Brandão-Costa, Thiago Pajeu Nascimento, Raquel Pedrosa Bezerra, Ana L.F. PortoAbstractFilicium decipiens saponin (FDS) is the first saponin purified from F. decipiens seeds using ion-exchange and gel filtration chromatographies. In the present study, FDS and the aqueous crude extract of F. decipiens seeds were examined for their antifungal properties, hemolytic / cytotoxicity activity. The dried seeds were powdered and homogenized in 10% (w/v) for the preparation of crude extract. Sample obtained from Sephadex-LH-20 ...
Source: Process Biochemistry - October 17, 2019 Category: Biochemistry Source Type: research

Preparation of Photo-crosslinkable cinnamate modified hyaluronic acid for immobilization of horseradish peroxidase
Publication date: Available online 16 October 2019Source: Process BiochemistryAuthor(s): Raedah A.S. Alatawi, M. Monier, Nadia H. ElsayedAbstractIn this work, a photo-responsive hydrogel membrane based on cinnamate-modified hyaluronic acid (HA-CM) was developed and safely cross-linked under UV light curing. The obtained material was effectively utilized for immobilization of horseradish peroxidase (HRP) enzyme via encapsulation and entrapment strategy with efficiency above 95%. The prepared HA-CM biopolymer was investigated before the UV curing using instrumental and spectral techniques including Fourier transform infrared...
Source: Process Biochemistry - October 17, 2019 Category: Biochemistry Source Type: research

Inactivation and structural alteration of α-amylase by low-pressure carbon dioxide microbubbles
Publication date: Available online 16 October 2019Source: Process BiochemistryAuthor(s): Fumiyuki Kobayashi, Risa Nakajima, Asako Narai-Kanayama, Sachiko OdakeAbstractThe efficiency of low-pressure carbon dioxide microbubbles (CO2MB) to inactivate α-amylase was analysed kinetically, and structural alteration of α-amylase by CO2MB was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence analysis of tryptophan (Trp) residues. Activity and Trp fluorescence intensity of α-amylase treated by CO2MB decreased with increasing temperature, pressure and exposure time...
Source: Process Biochemistry - October 17, 2019 Category: Biochemistry Source Type: research

Extraction, purification and characterization of water-soluble polysaccharides from green walnut husk with anti-oxidant and anti-proliferative capacities
Publication date: Available online 15 October 2019Source: Process BiochemistryAuthor(s): Nianwu He, Xichuan Zhai, Xiaobin Zhang, Xiaowen Zhang, Xinjun WangAbstractThis work was designed to study the physicochemical properties, and bioactivity of green walnut husk polysaccharides (GWHP). The crude GWHP was fractionated into three fractions, namely, GWHP-1, GWHP-2 and GWHP-3, using Cellulose DEAE-52 and Sephadex G-150. HPLC analysis showed that the three fractions were heteropolysaccharides consisting primarily of glucose, galactose and arabinose with average molecular weights of 165.06, 179.64 and 255.75 kDa, respect...
Source: Process Biochemistry - October 16, 2019 Category: Biochemistry Source Type: research

A Study on Increasing Enzymatic Stability and Activity of Baliospermum montanum Hydroxynitrile lyase in Biocatalysis
Publication date: Available online 15 October 2019Source: Process BiochemistryAuthor(s): Nisha Jangir, Preeti, Santosh Kumar PadhiAbstractHNL catalysis is usually carried out in a biphasic solvent and at low pH to suppress the non-enzymatic synthesis of racemic cyanohydrins. However, enzyme stability under these conditions remain a challenge. We have investigated the effect of different biocatalytic parameters, i.e., pH, temperature, buffer concentrations, presence of stabilizers, organic solvents, and chemical additives on the stability of Baliospermum montanum hydroxynitrile lyase (BmHNL). Unexpectedly, glycerol (50&thin...
Source: Process Biochemistry - October 16, 2019 Category: Biochemistry Source Type: research

Autocatalytic hydrothermal pretreatment by recycling byproduct organic acids to directionally depolymerize cassava straw
Publication date: Available online 14 October 2019Source: Process BiochemistryAuthor(s): Huisheng Lyu, Ziwei Zhai, Jinyi Zhou, Jia Zhang, Chunliu Lyu, Zhi Wang, Zhongfeng GengAbstractA two-stage autocatalytic hydrothermal pretreatment was proposed to improve the cassava straw utilization. The two-stage hydrothermal pretreatment was a process of which the first stage adopted lower-severity conditions (temperature and time) to improve the C-5 sugar yields and the second stage employed more severities to enhance C-6 sugar yield during enzyme hydrolysis. After employing this process, the maximum yields of C-5 and C-6 sugars we...
Source: Process Biochemistry - October 16, 2019 Category: Biochemistry Source Type: research

Gastrointestinal survival and potential bioactivities of Lactobacillus curieae CCTCC M2011381 in the fermentation of plant food
Publication date: Available online 13 October 2019Source: Process BiochemistryAuthor(s): Yafan Liu, Yiji Zhang, Kum-Song Ro, Honglin Li, Lixin Wang, Jingli Xie, Dongzhi WeiAbstractLactobacillus curieae CCTCC M2011381 is a novel strain isolated from stinky tofu brine. In order to evaluate the potential of the strain in fermenting plant foodstuff and possible bioactivity produced in fermentation, the growth of the strain in soymilk, soy protein isolate and ginkgo nut beverages was studied firstly. Cell counts of the strain could increase 4 log CFU/mL after 20 h of growth in all materials. The scavenging ratio of diphe...
Source: Process Biochemistry - October 14, 2019 Category: Biochemistry Source Type: research

A one-pot two-enzyme system on the production of high value-added D-allulose from Jerusalem artichoke tubers
Publication date: Available online 12 October 2019Source: Process BiochemistryAuthor(s): Ping Zhu, Yan Zeng, Peng Chen, Yan Men, Jiangang Yang, Xiaoping Yue, Jiangang Zhang, Yueming Zhu, Yuanxia SunAbstractA one-pot two-enzyme reaction system was developed to produce high-fructose syrup (HFS) containing healthy rare sugar d-allulose from Jerusalem artichoke (JA). Inulin in JA was converted through the cascade reaction of a novel exo-inulinase from Bacillus velezensis (BvInu) and D-allulose 3-epimerase from Ruminococcus sp. (RDAE). BvInu and RDAE were expressed in Bacillus subtilis and successfully secreted into the superna...
Source: Process Biochemistry - October 13, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: November 2019Source: Process Biochemistry, Volume 86Author(s): (Source: Process Biochemistry)
Source: Process Biochemistry - October 13, 2019 Category: Biochemistry Source Type: research

Preparation of a recyclable novel thermoresponsive affinity copolymer and its application towards ε-polylysine purification
Publication date: Available online 9 October 2019Source: Process BiochemistryAuthor(s): Fuhao Yang, Junfen Wan, Xuejun CaoAbstractThermo-responsive polymers have great potential for industrial applications of bio-separation and purification. However, the poor affinity and low recovery of thermoresponsive copolymers are the main factors limiting their large-scale application. In this paper, a recyclable thermoresponsive affinity copolymer (PNHM-IDA-Ni2+) was prepared by immobilizing nickel ions (Ni2+) on copolymer PNHM, and PNHM-IDA-Ni2+ was applied to the purification of ε-polylysine. The lower critical solution te...
Source: Process Biochemistry - October 10, 2019 Category: Biochemistry Source Type: research

Lignocellulosic conversion into value-added products: A review
Publication date: Available online 8 October 2019Source: Process BiochemistryAuthor(s): Dibyajyoti Haldar, Mihir Kumar PurkaitAbstractIn the present context of energy crisis, exploration of lignocellulosic biomass has emerged as potential substitute to maintain environmental sustainability. However, the conversion of biomass into value-added products still faces challenges to find a suitable unit operation. The stubborn dependency on the cost intensive enzymatic system, limits an effective saccharification to hydrolyze the biomass. India has been one of the top most agriculturally enriched countries with broad scope of uti...
Source: Process Biochemistry - October 9, 2019 Category: Biochemistry Source Type: research

Arthrospira (Spirulina) platensis: an effective biosorbent for nutrients
Publication date: Available online 8 October 2019Source: Process BiochemistryAuthor(s): Izabela Michalak, Małgorzata Mironiuk, Katarzyna Godlewska, Justyna Trynda, Krzysztof MaryczAbstractArthrospira (Spirulina) platensis was tested for biosorption properties. Preliminary experiments concerning biosorption kinetics were performed on Cr(III) ions. Equilibrium of biosorption was tested for Cr(III), Mn(II) and Mg(II) ions, since these elements are crucial for animals with metabolic disorders. In our study, Spirulina was proposed as a feed additive for animals suffering from diseases characterized by insulin dysregulation, ab...
Source: Process Biochemistry - October 9, 2019 Category: Biochemistry Source Type: research

Efficient and environmentally friendly method for carotenoid extraction from Paracoccus carotinifaciens utilizing naturally occurring Z-isomerization-accelerating catalysts
This study aimed to improve the efficiency of Paracoccus carotinifaciens-derived carotenoid (astaxanthin, adonirubin, adonixanthin) extraction using environmentally friendly Z-isomerization-accelerating catalysts. Adding naturally occurring catalysts such as isothiocyanates and polysulfides to the extraction solvent significantly improved the efficiency of carotenoid extraction, likely because of enhanced solubility of carotenoid Z-isomers compared with all-E-isomers. Indeed, addition of the catalysts markedly increased the content of carotenoid Z-isomers in the resulting extract. Addition of the catalyst allyl isothiocyan...
Source: Process Biochemistry - October 6, 2019 Category: Biochemistry Source Type: research

Facile hydrothermal synthesis of nitrogen rich blue fluorescent carbon dots for cell bio-imaging of Candida albicans
Publication date: Available online 5 October 2019Source: Process BiochemistryAuthor(s): Thomas Nesakumar Jebakumar Immanuel Edison, Raji Atchudan, Namachivayam Karthik, Dangsheng Xiong, Yong Rok LeeAbstractNitrogen doped carbon dots (N-CDs) are well documented as an outstanding fluorogenic material for protein tags, live cell imaging and protein-receptor based fluorescence sensors owing to its good optical features with less cytotoxicity and better water solubility. In this regard, the present work describes the synthesis of nitrogen rich blue fluorescent carbon dots (NR-CDs) through hydrothermal treatment of citric acid m...
Source: Process Biochemistry - October 6, 2019 Category: Biochemistry Source Type: research

Mg2+ distribution in activated sludge and its effects on the nitrifying activity and the characteristics of extracellular polymeric substances and sludge flocs
Publication date: Available online 5 October 2019Source: Process BiochemistryAuthor(s): Lanhe Zhang, Qiannan Zhao, Mingshuang Zhang, Jingbo Guo, Jing Zheng, Zicheng Chen, Yanping Jia, Jian Zhang, Zheng Li, Haifeng ZhangAbstractDivalent cations act as bridges among extracellular polymeric substances (EPS) and form cross-linkage for the self-immobilization of microbial biomass. However, their effects on the nitrification performance during the biological nitrogen removal are still unclear. In the present study, the effects of Mg2+ on the nitrifying activity, EPS and floc characteristics were investigated using a lab-scale se...
Source: Process Biochemistry - October 6, 2019 Category: Biochemistry Source Type: research

Hypoglycemic benefit and potential mechanism of a polysaccharide from Hericium erinaceus in streptozotoxin-induced diabetic rats
In this study, the hypoglycemic effect and possible mechanism of a polysaccharide, HEP-C, isolated from the fruit body of Hericium erinaceus were evaluated in streptozotoxin (STZ)-induced diabetic rats. Compared with the untreated STZ-induced diabetic rats, the supplements with HEP-C (150 and 300 mg/kg body weight [BW]) could significantly and dose-dependently relieve BW loss and organ injures, reduce fasting blood glucose, enhance glucose tolerance, alleviate hepatic function and serum lipid metabolism, elevate antioxidant enzyme activities, and suppress lipid peroxidation, which contributed to its potent hypoglyce...
Source: Process Biochemistry - October 4, 2019 Category: Biochemistry Source Type: research

Human serum albumin interaction, in silico and anticancer evaluation of pine-gold nanoparticles
Publication date: Available online 3 October 2019Source: Process BiochemistryAuthor(s): K. Anand, R. Rajamanikandan, A. Selva Sharma, M. Ilanchelian, Faez Iqbal Khan, C. Tiloke, Naresh Kumar Katari, P. Boomi, M. Saravanan, S. Palanisamy, M. Ramesh, C. Balakumar, Dakun Lai, A.A. ChuturgoonAbstractUnique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles (AuNPs) of 16 nm in size were generated using aqueous extracts of pine park (PB) and further complexed with human serum albumin (H...
Source: Process Biochemistry - October 4, 2019 Category: Biochemistry Source Type: research

Integration of metabolomic and transcriptomic profiling to compare two protocols of differentiation of human induced pluripotent stem cells into hepatocytes
Publication date: Available online 30 September 2019Source: Process BiochemistryAuthor(s): Rachid Jellali, Stephane Poulain, Myriam Lereau Bernier, Françoise Gilard, Yannick Tauran, Sachi Kato, Mathieu Danoy, Bertrand David Segard, Taketomo Kido, Atsushi Miyajima, Charles Plessy, Yasuyuki Sakai, Eric LeclercAbstractHuman hepatocyte-like cells derived from human induced pluripotent stem cells (hiPSC) may provide an unlimited supply of cells for in vitro liver models. However, hiPSC differentiation remains a major challenge due to immaturity of the hepatocytes obtained and the high cost of differentiation protocols cu...
Source: Process Biochemistry - October 1, 2019 Category: Biochemistry Source Type: research

High-performance centrifugal partition chromatography (HPCPC) for efficient isolation of diphlorethohydroxycarmalol (DPHC) and screening of its antioxidant activity in a zebrafish model
Publication date: Available online 30 September 2019Source: Process BiochemistryAuthor(s): Hyun-Soo Kim, Lei Wang, Thilina U. Jayawardena, Eun-A Kim, Soo-Jin Heo, I.P. Shanura Fernando, Ji-Hyeok Lee, You-Jin JeonAbstractHigh-performance centrifugal partition chromatography (HPCPC) can be used for the rapid isolation of biologically active metabolites from natural sources. The present study investigates the one-step isolation of diphlorethohydroxycarmalol (DPHC), an algal polyphenol, from the brown seaweed Ishige okamurae by HPCPC and its protective effect against 2,2’-azobis dihydrochloride (AAPH) induced oxidative s...
Source: Process Biochemistry - October 1, 2019 Category: Biochemistry Source Type: research

Preparation of silver nanoparticles and riboflavin embedded electrospun polymer nanofibrous scaffolds for in vivo wound dressing application
Publication date: Available online 27 September 2019Source: Process BiochemistryAuthor(s): Suganya Bharathi Balakrishnan, Stalin ThambusamyAbstractBiocompatible silver-based nanofibrous frameworks have attracted intensive attention in wound dressing materials ascribed to their greater stability, minimal toxicity, excellent antibacterial activity, and extended therapeutic efficiency. The present investigation delineates a simple approach to synthesize silver nanoparticles (Ag NPs), and riboflavin (RF) decorated polyvinyl alcohol/β-Cyclodextrin (PVA/β-CD) electrospun nanofibrous scaffolds envisioning their applicat...
Source: Process Biochemistry - September 28, 2019 Category: Biochemistry Source Type: research

Induction of browning and antioxidant capacity of cooked shark meats by pre-treatment with fermented rice bran suspension
Publication date: Available online 27 September 2019Source: Process BiochemistryAuthor(s): Junna Shibayama, Takashi Kuda, Hajime Takahashi, Bon KimuraAbstractPre-treatment of shark meats in 10% w/v sucrose/5% NaCl with rice bran suspension (RB) fermented by Saccharomyces cerevisiae Misaki-SU1 and Lactobacillus plantarum Sanriku-SU8 (FRB) improves the flavour and taste. In the present study, effects of RB and FRB pre-treatments on the browning (Maillard reaction) and antioxidant capacity of retorted (115 °C for 15 min) shark meats. Browning of the meat was suppressed and promoted by RB and FRB, respectivel...
Source: Process Biochemistry - September 28, 2019 Category: Biochemistry Source Type: research

Corrigendum to “Use of deep eutectic solvents in the enzyme catalysed production of ethyl lactate” [Process Biochem. 84 (2019) 53–59]
Publication date: Available online 26 September 2019Source: Process BiochemistryAuthor(s): Azime Arıkaya, Ayşe Ezgi Ünlü, Serpil Takaç (Source: Process Biochemistry)
Source: Process Biochemistry - September 27, 2019 Category: Biochemistry Source Type: research

Protein hydrolysate from Organic Fraction of Municipal Solid Waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826
Publication date: Available online 25 September 2019Source: Process BiochemistryAuthor(s): Jon Kepa Izaguirre, Thomas Dietrich, M. Carmen Villarán, Sonia CastañónAbstractIn this work a strategy for obtaining free amino-acids concentrate from an organic fraction of municipal solid waste compost and its use as a nitrogen source for lactic acid production, a compound widely used in different industries, using L.fermentumATCC 9338 and L.plantarum NCIMB 8826 strains is described. Enzymatic digestion is based on the combined action of endoprotease Alcalase 1.5 MG and exoprotease Flavourzyme 500&thinsp...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

Preparation of bovine hemoglobin surface molecularly imprinted cotton for selective protein recognition
Publication date: Available online 26 September 2019Source: Process BiochemistryAuthor(s): Pei Wang, Xu Tang, Lingquan Hu, Yankun Yin, Shaohua Chen, Jing Xu, Hua WangABSTRACTWe have developed a bovine hemoglobin (BHb) surface molecularly imprinted cotton based on degreasing cotton via surface imprinting technique for the efficient selective adsorption of BHb. The morphological structure of the samples was characterized by Scanning Electron Microscopy (SEM), and the chemical modification steps were characterized by Fourier Transform Infrared Spectroscopy (FTIR). The maximum adsorption capacity of the molecularly imprinted c...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

Enzymatic preparation and identification of 5′-adenosyl-methylthiopropylamine for the impurity control in SAM fermentation
Publication date: Available online 10 September 2019Source: Process BiochemistryAuthor(s): Dandan Tang, Feng Shi, Xiuliang Yang, Wei Liu, Weijun Zhao, Ruiqing Xian, Lei Huang, Leming Cheng, Zhinan XuAbstractS-adenosyl-L-methionine (SAM) is an important biosynthetic drug used for various diseases such as depression, arthritis syndromes and cirrhosis in clinical therapeutics. In order to ensure the high quality of SAM as a clinical drug, the key impurity, 5′-adenosyl-methylthiopropylamine (decarboxylated SAM, dC-SAM) should be monitored during the whole production process and in end tablet product. In the present work,...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

Sinensetin isolated from Orthosiphon aristatus inhibits cell proliferation and induces apoptosis in hepatocellular carcinoma cells
Publication date: Available online 24 September 2019Source: Process BiochemistryAuthor(s): Divakar Samidurai, Ashok Kumar Pandurangan, Senthil KumarKrishnamoorthi, Madan Kumar Perumal, Raaman NanjianAbstractOrthosiphon aristatus is a popular traditional folk medicine extensively used in Southeast Asia for its various pharmacological effects, including antioxidant, antitumor, and hypoglycemic activities. Orthosiphon extracts have been found to be cytotoxic to hepatocellular carcinoma (HCC) cells, which is attributed to their phytochemical content. However, the mechanism of action underlying the cytotoxic effects of Sinenset...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

A novel thermophilic β-mannanase with broad-range pH stability from Lichtheimia ramosa and its synergistic effect with α-galactosidase on hydrolyzing palm kernel meal
In this study, a novel GH5 family β-mannanase gene (LrMan5B) with 381 amino acid residues was identified from Lichtheimia ramosa, and highly expressed in Pichia pastoris X33. The amino acid sequence shares the highest identity (64%) with the β-mannanase from Rhizomucor miehei. Purified recombinant LrMan5B showed the optimal activity at pH 5.0 and 65 °C. It had broad-range pH stability (retaining>65% activity after incubation at pH 3.0–8.0 at 37 °C for 24 h) and was highly thermostable (retaining>80% activity after incubation at 60 °C for 30 min). LrMan5B ...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

Biochemical insights into a novel thermo/organo tolerant bilirubin oxidase from Thermosediminibacter oceani and its application in dye decolorization
Publication date: Available online 24 September 2019Source: Process BiochemistryAuthor(s): Issa Sadeghian, Zahra Rezaie, Seyyed Soheil Rahmatabadi, Shiva HemmatiAbstractHigher activity and stability at neutral pH and tolerance toward anions have made bilirubin oxidases (BODs) proper candidates for industrial utilizations. A putative BOD from Thermosediminibacter oceani (ToBOD) exhibited high stability over a pH range of 3.5-10.0 after heterologous expression and purification. The optimal temperature for the enzyme activity was 75 ˚C. ToBOD displayed a high thermostability with a half-life of 180 min at 70 ˚C and 120 min ...
Source: Process Biochemistry - September 26, 2019 Category: Biochemistry Source Type: research

In vitro antibacterial effect analysis of stabilized PEGylated allicin-containing extract from Allium sativum in conjugation with other antibiotics
Publication date: Available online 22 September 2019Source: Process BiochemistryAuthor(s): Souptik Bhattacharya, Dwaipayan Sen, Chiranjib BhattacharjeeAbstractAllicin is an organosulfur plant defensin mimetic compound, responsible for the bacteriostatic properties of Allium sativum (garlic). It is a major pharmaceutically important phytochemical that is capable of several biological activities including wide spectrum antibiotic action and synergy. However, the most crucial issue with the compound is its stability. Hence, the present study mainly focused on enhancing the stability of allicin for its commercial applicability...
Source: Process Biochemistry - September 24, 2019 Category: Biochemistry Source Type: research

Valorization of corn cob for the obtention and purification of endoglucanase produced by SSF
Publication date: Available online 22 September 2019Source: Process BiochemistryAuthor(s): María Julia Boggione, María Belén Allasia, Cristóbal Noé Aguilar, Beatriz FarruggiaAbstractAgro-food by-products contain valuable nutrients which are wasted. In this work, solid-state fermentation (SSF) was carried out using corn cob as substrate for endoglucanase production. The radial growth of three fungal strains -Trichoderma harzianum T104, Aspergillus niger GH1 and Aspergillus niger NRRL3- was analyzed in order to select the most appropriate. Radial growth data were analyzed with a mixed linea...
Source: Process Biochemistry - September 24, 2019 Category: Biochemistry Source Type: research

Green approach synthesis of Pd@TiO2 nanoparticles: characterization, visible light active picric acid degradation and anticancer activity
Publication date: Available online 21 September 2019Source: Process BiochemistryAuthor(s): D. Hariharan, P. Thangamuniyandi, P. Selvakumar, U. Devan, A. Pugazhendhi, R. Vasantharaja, L.C. NehruAbstractTitanium dioxide (TiO2) and palladium (Pd) doped TiO2 (Pd@TiO2) nanoparticles have been synthesized using green approach technique. X-ray diffraction analysis reveals the formation of anatase phases for both pure TiO2 and Pd@TiO2. The optical properties confirm that the synthesized Pd@TiO2 eventually absorbs visible light. High resolution transmission electron microscopy analysis shows that the tiny dots seen all over the sur...
Source: Process Biochemistry - September 22, 2019 Category: Biochemistry Source Type: research

Metabolite profiling, molecular docking and in vitro anticancer potential of marine ascidian Didemnum sp
Publication date: Available online 21 September 2019Source: Process BiochemistryAuthor(s): Velusamy Arumugam, Manigandan Venkatesan, Selvakumar Murugesan, Rathinam Ayyasamy, Saravanan Ramachandran, Umamaheswari Sundaresan, Arivalagan PugazhendhiAbstractMarine ascidians contain potential bioactive compounds, which exhibit significant pharmacological activities. In the present study, the MeOH extract of Didemnum sp. was subjected to FT-IR, GC-MS analysis, the possible interactions with EGFR through docking analysis and in vitro cytotoxic activity against KB (Human oral cancer) cell line were also studied. The FT-IR analysis ...
Source: Process Biochemistry - September 22, 2019 Category: Biochemistry Source Type: research

Partitioning of tylosin in recyclable aqueous two-phase systems based on two pH-responsive polymers
Publication date: Available online 19 September 2019Source: Process BiochemistryAuthor(s): Shan Bai, JunFen Wan, XueJun CaoAbstractAqueous two-phase systems (ATPS) are a promising biotechnology in bioseparation. However, recycling of the phase-forming polymer is a key issue. In this research, new recyclable ATPS composed of two pH-responsive polymers (PADB4.01 and PMDBH3.27) were prepared. The ATPS could be recycled by adjusting the pH of the solution, with recovery of 93.8%. The ATPS were evaluated for the first time in the partitioning of tylosin. The effects of the main parameters, such as the polymer concentration, pH,...
Source: Process Biochemistry - September 20, 2019 Category: Biochemistry Source Type: research

Enzymatic glycolipid surfactant synthesis from renewables
Publication date: Available online 20 September 2019Source: Process BiochemistryAuthor(s): Jens Grüninger, André Delavault, Katrin OchsenreitherAbstractGlycolipids are promising nonionic biosurfactants with a wide range of application. In contrast to the traditional surfactants from fossil origin, they are not only exhibiting excellent surfactant and emulsifying properties but are in addition biodegradable and non-toxic to the environment. Glycolipids can be produced by microbial fermentation, by chemical or enzymatical synthesis using renewable resources. In this review, strengths and weaknesses of each produc...
Source: Process Biochemistry - September 20, 2019 Category: Biochemistry Source Type: research

Effects of a nonionic surfactant TX-40 on 9α-hydroxyandrost-4-ene-3,17-dione biosynthesis and physiological properties of Mycobacterium sp. LY-1
Publication date: Available online 16 September 2019Source: Process BiochemistryAuthor(s): Longfei Zhou, Hui Li, Yanan Xu, Wei Liu, Xiaomei Zhang, Jinsong Gong, Zhenghong Xu, Jinsong ShiAbstractThe 9α-hydroxyandrost-4-ene-3,17-dione (9α-OH-AD) is a key intermediate for synthetizing steroid pharmaceutical compounds. It is bioconverted by Mycobacterium sp. LY-1 from phytosterols. During biotransformation, the low solubility of phytosterols in water limits 9α-OH-AD production. In the present work, polyoxyethylene (10) nonylphenyl ether (TX-40), a nonionic surfactant, was first used to enhance phytosterol sol...
Source: Process Biochemistry - September 18, 2019 Category: Biochemistry Source Type: research

Ultrasound-assisted fractional precipitation of paclitaxel from Taxus chinensis cell cultures
In this study, we developed an ultrasound-assisted fractional precipitation for the purification of poorly water-soluble anticancer agent paclitaxel. The effects of ultrasound power (80, 180, 250, and 380 W) were investigated in samples of different purity (20.4, 63.6, and 92.7%) for precipitation. Higher precipitation rate and shorter precipitation time were found with ultrasound treated solutions than with those without ultrasound. When the experimental data were applied to the Johnson-Mehl-Avrami-Komolgorov (JMAK) equation, the rate constant increased to 200-500-fold for 20.4% of sample purity, 150-250-fold for 6...
Source: Process Biochemistry - September 18, 2019 Category: Biochemistry Source Type: research

Development and comparative analysis of human urine exosome isolation strategies
In this study, comparative studies among eight different strategies based on ultracentrifugation, ultrafiltration, precipitation, and size exclusion chromatography (SEC) were performed for urinary exosome isolation from healthy donors. The results indicated that SEC combined with ultrafiltration was the most suitable method for urinary exosome isolation in terms of both isolation yield and purity. SEC combined with precipitation during urinary exosome isolation yielded the highest purity. Thus, the appropriate urinary exosome isolation method can be chosen depending on the downstream applications of isolated urinary exosom...
Source: Process Biochemistry - September 16, 2019 Category: Biochemistry Source Type: research

Regioselective hydroxylation pathway of tenatoprazole to produce human metabolites by Bacillus megaterium CYP102A1
In conclusion, an enzymatic synthesis of 5’-OH tenatoprazole, a major human metabolite of tenatoprazole, was developed by using mutants of CYP102A1 from Bacillus megaterium as a biocatalyst and tenatoprazole as a substrate.Graphical abstract (Source: Process Biochemistry)
Source: Process Biochemistry - September 15, 2019 Category: Biochemistry Source Type: research

Biomolecules from extremophile microalgae: from genetics to bioprocessing of a new candidate for large-scale production
Publication date: Available online 13 September 2019Source: Process BiochemistryAuthor(s): Eduardo Bittencourt Sydney, Kathlyn Schafranski, Barbara Ruivo Valio Barreti, Alessandra Cristine Novak Sydney, Jéssika Fernandes D’Arc Zimmerman, Maria Luísa Cerri, Ivo Mottin DemiateAbstractExtremophile microalgae are potential candidates for large-scale production because the extreme growth conditions help avoiding contamination, especially in open pond reactors. Considering the role of microalgae cultivation in integrated production systems, especially reusing wastewater and gaseous effluents, the search for n...
Source: Process Biochemistry - September 15, 2019 Category: Biochemistry Source Type: research

Development of a regulatory defined medium using a system-oriented strategy to reduce the intracellular constraints
Publication date: Available online 13 September 2019Source: Process BiochemistryAuthor(s): Ehsan Motamedian, Maryam Sarmadi, Elahe DerakhshanAbstractDifficulties associated with genetic manipulation and restrictions on the use of genetically modified organisms limit the application of metabolic engineering for improving the productivity of a cell. In this research, a system-oriented strategy was proposed to develop a regulatory defined medium (RDM) that removes the intracellular constraints of a cell system without any genetic manipulation. A transcriptional regulated metabolic model was first applied to identify the minim...
Source: Process Biochemistry - September 15, 2019 Category: Biochemistry Source Type: research

Repeated-batch lactic acid fermentation using a novel bacterial immobilization technique based on a microtube array membrane
Publication date: Available online 13 September 2019Source: Process BiochemistryAuthor(s): Chien-Chung Chen, Chuan-Chi Lan, Chorng-Liang Pan, Mei-Ying Huang, Chee-Ho Chew, Chin-Chieh Hung, Po-Hsuan Chen, Hong-Ting Victor LinAbstractLactic acid has received considerable attention for its various applications. Although lactic acid is usually produced by batch fermentation using free microbes, repeated-batch fermentation using immobilized cells could offer several advantages over this method. In the present study, a novel bacteria immobilization technique using a renewable poly-L-lactic acid (PLLA) microtube array membrane (M...
Source: Process Biochemistry - September 15, 2019 Category: Biochemistry Source Type: research

Madhuca indica flower extract as cheaper carbon source for production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) using Ralstonia eutropha
In this study, the poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized using Madhuca indica flower extract as cheaper carbon source by Ralstonia eutropha. The Influence of fermentation parameters such as incubation time, temperature, pH and inoculum size were studied on production of PHBV. The maximum production of PHBV (1.44 ± 0.016 g/L with yield of 49.4 %) was obtained at a temperature, pH, inoculum size and incubation time of 35 °C, 6, 5 % v/v and 48 h, respectively. The PHBV was characterized using nuclear magnetic resonance spectroscopy (NMR), Fourier tra...
Source: Process Biochemistry - September 15, 2019 Category: Biochemistry Source Type: research

Microbial Communities Variation Analysis of Denitrifying Bacteria Immobilized Particles
Publication date: Available online 12 September 2019Source: Process BiochemistryAuthor(s): Liangang Hou, Jun Li, Yang LiuAbstractThe aim of this study was to investigate the denitrification performance and analyze the variation of microbial communities in immobilized particles which used in real municipal wastewater treatment. The bioreactor has achieved good denitrification effect on real municipal wastewater treatment, the NO3--N and TN removal efficiencies reached 77.4% and 68.3%, respectively. High-throughput sequencing shows that the community structure and relative abundance of bacteria changed greatly, especially at...
Source: Process Biochemistry - September 13, 2019 Category: Biochemistry Source Type: research

Effect of different homogenization pressure on soy protein isolate-vitamin D3 complex
Publication date: Available online 11 September 2019Source: Process BiochemistryAuthor(s): Anqi Zhang, Shuang Chen, Yuying Wang, Guowei Zhou, Lin Wang, Xibo Wang, Ning XuAbstractSoy protein isolate (SPI) and vitamin D3 (VD3) interaction may be a way to improve the stability of VD3. In this paper, SPI-VD3 nanocomposites were prepared by using soy protein isolate and VD3 under different homogenization pressure conditions to study the changes of SPI structure and VD3 stability in the complex. When the homogenization pressure was 100 MPa, the encapsulation efficiency, loading rate and average particle size of the SPI-VD...
Source: Process Biochemistry - September 12, 2019 Category: Biochemistry Source Type: research

Enzymatic preparation and identification of 5’-adenosyl-methylthiopropylamine for the impurity control in SAM fermentation
Publication date: Available online 10 September 2019Source: Process BiochemistryAuthor(s): Dandan Tang, Feng Shi, Xiuliang Yang, Wei Liu, Weijun Zhao, Ruiqing Xian, Lei Huang, Leming Cheng, Zhinan XuAbstractS-adenosyl-L-methionine (SAM) is an important biosynthetic drug used for various diseases such as depression, arthritis syndromes and cirrhosis in clinical therapeutics. In order to ensure the high quality of SAM as a clinical drug, the key impurity, 5’-adenosyl-methylthiopropylamine (decarboxylated SAM, dC-SAM) should be monitored during the whole production process and in end tablet product. In the present work,...
Source: Process Biochemistry - September 10, 2019 Category: Biochemistry Source Type: research

Supercritical carbon dioxide extraction of α-mangostin from mangosteen pericarp with virgin coconut oil as co-extractant and in-vitro bio-accessibility measurement
Publication date: Available online 9 September 2019Source: Process BiochemistryAuthor(s): Wan Jun Lee, Chea Chi Ng, Jin Shuen Ng, Richard Lee Smith, Siew Lee Kok, Yen Yi Hee, Sin Yee Lee, Wei Kiat Tan, Nur Hanani Zainal Abidin, Sarina Abdul Halim Lim, Gun Hean ChongAbstractThis work aims to develop a method for producing α-mangostin that has high bio-accessibility through the use of supercritical carbon dioxide (scCO2) extraction of mangosteen pericarp extract (MPE) with virgin coconut oil (VCO) as co-extractant. Extractions with scCO2 were carried out at 30 MPa to 40 MPa, 60 °C, CO2 flow rat...
Source: Process Biochemistry - September 10, 2019 Category: Biochemistry Source Type: research

Effect of multi-frequency counter-current S type ultrasound pretreatment on the enzymatic hydrolysis of defatted corn germ protein: Kinetics and thermodynamics
Publication date: Available online 8 September 2019Source: Process BiochemistryAuthor(s): Abubakr Musa, Haile Ma, Mohammed A.A. Gasmalla, Frederick Sarpong, Faisal N. Awad, Yuqing DuanAbstractIn this paper, the impact of ultrasound pretreatment on the kinetics and thermodynamics of defatted corn germ protein (DCGP) enzymatic hydrolysis was evaluated. The enzymatic hydrolysis kinetics and thermodynamics were studied for traditional method and ultrasonic pretreatments with a single-frequency counter-current S type (20 KHz) (SFU) and a multi-frequency counter-current S type (20, 28, 35 and 40 KHz) (MFU). The results showed th...
Source: Process Biochemistry - September 8, 2019 Category: Biochemistry Source Type: research