First report on prevalence, molecular characterization and phylogenetic study of Toxoplasma gondii infecting sheep of the Malakand Division of Pakistan
Comp Immunol Microbiol Infect Dis. 2024 Apr 18;109:102184. doi: 10.1016/j.cimid.2024.102184. Online ahead of print.ABSTRACTBACKGROUND: Toxoplasma gondii is an apicomplexan protozoan parasite that infects one-third of the population of the world, including humans, animals, birds, and other vertebrates. The present investigation is the first molecular attempt in the Malakand Division of Pakistan to determine the epidemiology and phylogenetic study of Toxoplasma gondii infecting small ruminants.METHODOLOGY: A total of (N = 450) blood samples of sheep were randomly collected during the study period (December 2020 to November 2...
Source: Comparative immunology, microbiology and infectious diseases. - May 1, 2024 Category: Allergy & Immunology Authors: Ahmad Irshad Shumaila Noreen Faisal Nouroz Akhtar Rasool Mingkun Zhu Mohamed Mohany Source Type: research
Bacterial burden and molecular characterization of Coxiella burnetii in shedding pregnant and postpartum ewes from Saint Kitts
This study aimed to evaluate the bacterial burden and perform molecular characterization of Coxiella burnetii during shedding in pregnant (vaginal, mucus and feces) and postpartum (vaginal mucus, feces and milk) ewes from Saint Kitts. Positive IS1111 DNA (n=250) for C. burnetii samples from pregnant (n=87) and postpartum (n=74) Barbados Blackbelly ewes in a previous investigation were used for this study. Vaginal mucus (n=118), feces (n=100), and milk (n=32) positive IS1111 C. burnetii-DNA were analysed by real time qPCR (icd gene). For molecular characterization of C. burnetii, selected (n=10) IS1111 qPCR positive samples...
Source: Comparative immunology, microbiology and infectious diseases. - May 1, 2024 Category: Allergy & Immunology Authors: Mayra Trujillo Anne Conan Ana Cl áudia Calchi Katja Mertens-Scholz Anna Becker Christa Gallagher Alex Mau Silvia Marchi Marcus Machado Marcos Rog ério André Aspinas Chapwanya Ananda M üller Source Type: research
Partners and guardians of the nervous and immune systems
Trends Immunol. 2024 Apr 30:S1471-4906(24)00076-0. doi: 10.1016/j.it.2024.04.007. Online ahead of print.NO ABSTRACTPMID:38692936 | DOI:10.1016/j.it.2024.04.007 (Source: Trends in Immunology)
Source: Trends in Immunology - May 1, 2024 Category: Allergy & Immunology Authors: Catarina Sacrist án Source Type: research
Analysis of tissue tropism of GCRV-II infection in grass carp using a VP35 monoclonal antibody
In this study, monoclonal antibodies against recombinant VP35 protein were generated in mice and characterized. The antibodies exhibited specific binding to the N terminal region (1-155 aa) of the recombinant VP35 protein expressed in the HEK293 cells, and native VP35 protein in the GCRV-II infected CIK cells. Immunofluorescent staining revealed that viruses aggregated in the cytoplasm of infected cells. In vivo challenge experiments showed that high levels of GCRV-II viruses were present in the gills, intestine, spleen and liver, indicating that they are the major sites for virus infection. Our study showed that the VP35 ...
Source: Developmental and Comparative Immunology - May 1, 2024 Category: Allergy & Immunology Authors: Yanan Lu Weihua Zhao Ning Ji Dan Xu Yaoguo Li Tiaoyi Xiao Junya Wang Jun Zou Source Type: research
Optimizing Spectronaut Search Parameters to Improve Data Quality with Minimal Proteome Coverage Reductions in DIA Analyses of Heterogeneous Samples
J Proteome Res. 2024 May 1. doi: 10.1021/acs.jproteome.3c00671. Online ahead of print.ABSTRACTData-independent acquisition has seen breakthroughs that enable comprehensive proteome profiling using short gradients. As the proteome coverage continues to increase, the quality of the data generated becomes much more relevant. Using Spectronaut, we show that the default search parameters can be easily optimized to minimize the occurrence of false positives across different samples. Using an immunological infection model system to demonstrate the impact of adjusting search settings, we analyzed Mus musculus macrophages and compa...
Source: Cell Research - May 1, 2024 Category: Cytology Authors: Christa P Baker Roland Bruderer James Abbott J Simon C Arthur Alejandro J Brenes Source Type: research
CD4 < sup > + < /sup > T and CD8 < sup > + < /sup > T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures
In this study, we fully compared the immunological properties of αβT cells between uterus and blood using mouse and human sample. It showed that most of CD4+T cells and CD8+T cells in murine uterus and human endometrium were tissue resident memory T cells which highly expressed tissue residence markers CD69 and/or CD103. In addition, both CD4+T cells and CD8+T cells in uterus highly expressed inhibitory molecular PD-1 and cytokine IFN-γ. Uterine CD4+T cells highly expressed IL-17 and modulated by transcription factor pSTAT3. Moreover, we compared the similarities and differences between human and murine uterine T cell p...
Source: Cell Research - May 1, 2024 Category: Cytology Authors: Shuangpeng Kang Shuiping Jin Xueying Mao BinSheng He Changyou Wu Source Type: research
CD4 < sup > + < /sup > T and CD8 < sup > + < /sup > T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures
In this study, we fully compared the immunological properties of αβT cells between uterus and blood using mouse and human sample. It showed that most of CD4+T cells and CD8+T cells in murine uterus and human endometrium were tissue resident memory T cells which highly expressed tissue residence markers CD69 and/or CD103. In addition, both CD4+T cells and CD8+T cells in uterus highly expressed inhibitory molecular PD-1 and cytokine IFN-γ. Uterine CD4+T cells highly expressed IL-17 and modulated by transcription factor pSTAT3. Moreover, we compared the similarities and differences between human and murine uterine T cell p...
Source: Journal of Immunology Research - May 1, 2024 Category: Allergy & Immunology Authors: Shuangpeng Kang Shuiping Jin Xueying Mao BinSheng He Changyou Wu Source Type: research
Neutrophils in pancreatic ductal adenocarcinoma: bridging preclinical insights to clinical prospects for improved therapeutic strategies
Expert Rev Clin Immunol. 2024 May 1. doi: 10.1080/1744666X.2024.2348605. Online ahead of print.ABSTRACTINTRODUCTION: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by a dismal five-year survival rate of less than 10%. Neutrophils are key components of the innate immune system, playing a pivotal role in the PDAC immune microenvironment.AREAS COVERED: This review provides a comprehensive survey of the pivotal involvement of neutrophils in the tumorigenesis and progression of PDAC. Furthermore, it synthesizes preclinical and clinical explorations aimed at targeting neutrophils within t...
Source: Expert Review of Clinical Immunology - May 1, 2024 Category: Allergy & Immunology Authors: Yi Jin Eric S Christenson Lei Zheng Keyu Li Source Type: research
Widespread Adoption of Microincision Vitrectomy Surgery Improves Visual Outcomes in Endogenous Endophthalmitis with Poor Initial Vision: A 21-Year Experience in Taiwan
CONCLUSION: In endogenous endophthalmitis patients presenting with poor initial visual acuity, vitrectomy was a better visual prognostic factor. MIVS has allowed more patients to undergo vitrectomy and improved visual outcomes.PMID:38691837 | DOI:10.1080/09273948.2024.2338272 (Source: Ocular Immunology and Inflammation)
Source: Ocular Immunology and Inflammation - May 1, 2024 Category: Allergy & Immunology Authors: Shih-Chou Chen Tsung-Tien Wu Chun-Hao Yin Jin-Shuen Chen Yao-Shen Chen Source Type: research
Adalimumab for the Treatment of Non-Infectious Uveitis: A Real Life Experience
CONCLUSIONS: We consider adalimumab as a leading drug in the treatment of NIU with high safety and efficacy.PMID:38691840 | DOI:10.1080/09273948.2024.2344712 (Source: Ocular Immunology and Inflammation)
Source: Ocular Immunology and Inflammation - May 1, 2024 Category: Allergy & Immunology Authors: F átima Raad Paula Luque Sof ía García Ledo Alicia Alda Lozano V íctor Llorens Antonio Espejo Henar Heras Luc ía Santana Luis Trapiella Patricia Fanlo Alfredo Ad án Gerard Espinosa Nuria Navarrete Source Type: research
Evaluation of Choroidal Thickness and Choroidal Vascularity Index in Patients with Rheumatoid Arthritis
CONCLUSION: We demonstrated thinning in CT and decreased CVI for the first time in RA patients by comparing it with the control group.PMID:38691844 | DOI:10.1080/09273948.2024.2345289 (Source: Ocular Immunology and Inflammation)
Source: Ocular Immunology and Inflammation - May 1, 2024 Category: Allergy & Immunology Authors: G ülce Gökgöz Özışık Dilek Eker Buyuksireci Tayfun Şahin Ayla Cagl ıyan Turk Source Type: research
Transport of glycinin, the major soybean allergen, across intestinal epithelial IPEC ‐J2 cell monolayers
The objective of this study was to elucidate the pathway and mechanism of glycinin hydrolysate transport through the intestinal epithelial barrier using IPEC-J2 cell model. Purified glycinin was digested by in vitro static digestion model. The pathway and mechanism of glycinin hydrolysates transport through intestinal epithelial cells were investigated by cellular transcytosis assay, cellul ar uptake assay, immunoelectron microscopy and endocytosis inhibition assay. The glycinin hydrolysates were transported across IPEC-J2 cell monolayers in a time/dose-dependent manner following the Michaelis equation. Immunoelectron mi...
Source: Journal of Animal Physiology and Animal Nutrition - May 1, 2024 Category: Zoology Authors: Ziang Zheng,
Yintong Zhao,
Shugui Zheng,
Yajin Liu,
Simiao Liu,
Junfeng Han Tags: ORIGINAL ARTICLE Source Type: research
Tibial Cortex Transverse Transport Facilitates Severe Diabetic Foot Wound Healing via HIF-1 α-Induced Angiogenesis
(Source: Journal of Inflammation Research)
Source: Journal of Inflammation Research - May 1, 2024 Category: Allergy & Immunology Tags: Journal of Inflammation Research Source Type: research
Hyperuricemia Increases the Risk of Postoperative Recurrence in Chinese Patients with Chronic Rhinosinusitis
(Source: Journal of Inflammation Research)
Source: Journal of Inflammation Research - May 1, 2024 Category: Allergy & Immunology Tags: Journal of Inflammation Research Source Type: research
Beyond Immune Balance: The Pivotal Role of Decidual Regulatory T Cells in Unexplained Recurrent Spontaneous Abortion
(Source: Journal of Inflammation Research)
Source: Journal of Inflammation Research - May 1, 2024 Category: Allergy & Immunology Tags: Journal of Inflammation Research Source Type: research
