Developmental and Comparative Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research

Effect of F-isoprostane class on cow peripheral blood neutrophil microbicidal function in vitro
Dev Comp Immunol. 2024 Apr 17;156:105180. doi: 10.1016/j.dci.2024.105180. Online ahead of print.ABSTRACTIsoprostanes (isoP) are formed during conditions of oxidative stress (OS) through the oxidation of cell membrane fatty acids. Different classes of isoP are formed depending on the fatty acid being oxidized but the biological activity of these molecules in innate immune cells is poorly understood. Thus, the objective of this study was to compare in vitro the effects of F2- and F3-isoP on neutrophil microbicidal functions. We isolated neutrophils from 6 dairy cows and incubated them for 8 h at various concentrations of F2-...
Source: Developmental and Comparative Immunology - April 19, 2024 Category: Allergy & Immunology Authors: Eric J Owczarzak Angel Abuelo Source Type: research

Fish ubiquitin-specific protease 8 (USP8) inhibits IFN production through autophagy-lysosomal dependent degradation of IRF7
In this study, we report that zebrafish ubiquitin-specific protease 8 (USP8) promotes IRF7 degradation through an autophagy-lysosome-dependent pathway to inhibit IFN production. First, zebrafish usp8 is induced upon spring viremia of carp virus (SVCV) infection and polyinosinic/polycytidylic acid (poly I:C) stimulation. Second, overexpression of USP8 suppresses SVCV or poly I:C-mediated IFN expression. Mechanistically, USP8 interacts with IRF7 and promotes its degradation via an autophagy-lysosome-dependent pathway. Finally, USP8 significantly suppresses cellular antiviral responses and enhances SVCV proliferation. In summ...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: Chu-Jing Zhou Can Zhang Long-Feng Lu Shun Li Source Type: research

Study on anti-BmNPV mechanism of branched-chain amino acid aminotransferases in silkworm
In this study, the four midgut tissue samples of the resistance strain NB (NB) and susceptible strain 306 (306) and the NB and 306 continuously infected with BmNPV for 96 h are used for whole transcriptome sequencing to analyze the differences in the genetic background of NB and 306 and the differences after inoculation of BmNPV, and the significantly different mRNA, miRNA and lnRNA between NB and 306 after BmNPV inoculation were screened. By comparing NB and 306, 2651 significantly different mRNAs, 57 significantly different miRNAs and 198 significantly different lncRNAs were screened. By comparing NB and 306 after BmNPV ...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: Can Chen Liang Chen Xiaoyong Liu Shangshang Ma Keping Chen Source Type: research

Galectin-8-like isoform X1 mediates antibacterial, antiviral, and antioxidant responses in red-lip mullet (Planiliza haematocheilus) through positive modulation of pro-inflammatory cytokine, chemokine, and enzymatic antioxidant activity
This study identified Galectin-8-like isoform X1 (PhGal8X1) from red-lip mullet (Planiliza haematocheilus) and investigated its role in regulating fish immunity. The open reading frame of PhGal8X1 was 918bp, encoding a soluble protein of 305 amino acids. The protein had a theoretical isoelectric (pI) point of 7.7 and an estimated molecular weight of 34.078 kDa. PhGal8X1 was expressed in various tissues of the fish, with prominent levels in the brain, stomach, and intestine. PhGal8X1 expression was significantly (p<0.05) induced in the blood and spleen upon challenge with different immune stimuli, including polyinosinic:...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: W A D L R Warnakula H M V Udayantha D S Liyanage E M T Tharanga W K M Omeka M A H Dilshan H A C R Hanchapola J D H E Jayasinghe Taehyug Jeong Qiang Wan Jehee Lee Source Type: research

Study on anti-BmNPV mechanism of branched-chain amino acid aminotransferases in silkworm
In this study, the four midgut tissue samples of the resistance strain NB (NB) and susceptible strain 306 (306) and the NB and 306 continuously infected with BmNPV for 96 hours are used for whole transcriptome sequencing to analyze the differences in the genetic background of NB and 306 and the differences after inoculation of BmNPV, and the significantly different mRNA, miRNA and lnRNA between NB and 306 after BmNPV inoculation were screened. By comparing NB and 306, 2,651 significantly different mRNAs, 57 significantly different miRNAs and 198 significantly different lncRNAs were screened. By comparing NB and 306 after B...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: Can Chen Liang Chen Xiaoyong Liu Shangshang Ma Keping Chen Source Type: research

The marine sponge, Hymeniacidon sinapium, displays allorecognition of siblings during post-larval settling and metamorphosis to juveniles
Dev Comp Immunol. 2024 Apr 11:105179. doi: 10.1016/j.dci.2024.105179. Online ahead of print.ABSTRACTMarine sponges, including the crumb of bread sponge, Hymeniacidon sinapium, display allorejection responses to contact with conspecifics in both experimental and natural settings. These responses have been used to infer immunocompetence in a variety of marine invertebrates. However, larvae and juveniles released from several marine sponge species fuse and form chimeras. Some of these chimeras persist, whereas others eventually break down, revealing a period of allogeneic non-responsiveness that varies depending on the specie...
Source: Developmental and Comparative Immunology - April 13, 2024 Category: Allergy & Immunology Authors: L Courtney Smith Source Type: research

Identification and characterization of myeloid cells localized in the tadpole liver cortex in Xenopus laevis
Dev Comp Immunol. 2024 Apr 8:105178. doi: 10.1016/j.dci.2024.105178. Online ahead of print.ABSTRACTIn the present study, using transgenic frogs that express GFP specifically in myeloid cells under the myeloperoxidase enhancer sequence, we found that myeloperoxidase-positive cells are localized in the liver cortex at the late tadpole stages. Immunohistochemical analysis revealed that myelopoiesis in the liver cortex became evident after st. 50 and reached its peak by st. 56. Transplantation experiments indicated that cells with a high density at the liver cortex were derived from the dorso-lateral plate tissue in the neurul...
Source: Developmental and Comparative Immunology - April 10, 2024 Category: Allergy & Immunology Authors: Mitsugu Ma éno Miki Tanabe Ayame Ogawa Haruka Kobayashi Yumi Izutsu Takashi Kato Source Type: research

Glycosaminoglycan lyase: A new competition between bacteria and the pacific white shrimp Litopenaeus vannamei
In this study, we analyzed bacteria-derived HGT based on a high-quality genome of L. vannamei via a homology search and phylogenetic analysis, and six HGT genes were identified. Among these six horizontally transferred genes, we found one gene (LOC113799989) that contains a bacterial chondroitinase AC structural domain and encodes an unknown glycosaminoglycan (GAG) lyase in L. vannamei. The real-time quantitative PCR results showed that the mRNA expression level of LOC113799989 was highest in the hepatopancreas and heart, and after stimulation by Vibrio parahaemolyticus, its mRNA expression level was rapidly up-regulated w...
Source: Developmental and Comparative Immunology - April 9, 2024 Category: Allergy & Immunology Authors: Bo Zheng Gengzhuo Wang Zhe Qu Jingjie Hu Zhenmin Bao Mengqiang Wang Source Type: research