Age-related viral load and severity of systemic pathological lesions in acute naturally occurring African swine fever virus genotype II infections
The objective of this study was to determine the age-related viral loads and severity of systemic pathological lesions among three different swine group ages (weaned pigs, fattening pigs, and sows) during a recent outbreak of acute ASF in Vietnam. Age-related viral loads were determined in 5 major organs (lung, liver, spleen, kidney, and lymph node) by immunohistochemistry as well as in the blood by real-time polymerase chain reaction (PCR). Age-related systemic pathological lesions were analyzed in the listed organs among three age groups. Weaned pigs had significantly (p
Source: Comparative immunology, microbiology and infectious diseases. - September 20, 2021 Category: Allergy & Immunology Authors: Taehwan Oh Duy Tien Do Danh Cong Lai Thanh Che Nguyen Hung Van Vo Chanhee Chae Source Type: research

Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
Comp Immunol Microbiol Infect Dis. 2021 Aug 28;79:101697. doi: 10.1016/j.cimid.2021.101697. Online ahead of print.ABSTRACTMethicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as "true MRSA", is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureu...
Source: Comparative immunology, microbiology and infectious diseases. - September 16, 2021 Category: Allergy & Immunology Authors: S C L Santos M M S Saraiva A L B Moreira Filho N M V Silva C M G De Leon L A F Pascoal P E N Givisiez W A Gebreyes C J B Oliveira Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli
This study aimed at evaluate the presence and to study characteristics of Escherichia coli in the respiratory system microbiota of healthy broilers. Trachea, air sacs, and lungs of 20 broilers were analyzed at 21 days of age, reared in experimental conditions, without receiving antimicrobials. E. coli strains were isolated and identified using conventional bacteriology through morphological and biochemical characterization. The production of bacteriocin-like substances, the presence of virulence-associated genes (VAGs) of APEC (Avian Pathogenic Escherichia coli) predictors, and the antimicrobial susceptibility were evaluat...
Source: Comparative immunology, microbiology and infectious diseases. - September 10, 2021 Category: Allergy & Immunology Authors: Beatriz Dugaich Soares Kelly Cristina Tagliari de Brito Tiela Trapp Grassotti Hiran Castagnino Kunert Filho Taiara Carolaine Leal de Camargo Daiane Carvalho Izabela Camilotti Dorneles Luciana Kazue Otutumi Lissandra Souto Cavalli Benito Guimar ães de Bri Source Type: research

A novel enhanced dot blot immunoassay using colorimetric biosensor for detection of Toxoplasma gondii infection
This study reports development of a novel point of care assay, namely an enhanced immuno-dot blot assay, for discrimination of anti-Toxoplasma IgG and anti-Toxoplasma IgM antibodies. This method has been designed based on formation of a sandwich complex between a gold nanoprobe (chitosan gold nanoparticle-anti-human IgG or anti-IgM) and anti- Toxoplasma lysate antigen (TLA) which holds anti-TLA antibodies, either IgG or IgM. Briefly, anti-human IgG or anti-IgM antibody was conjugated to chitosan gold nanoparticles via glutaraldehyde chemistry. Then, lysate antigen was immobilized on the surface of nitrocellulose membrane, ...
Source: Comparative immunology, microbiology and infectious diseases. - September 4, 2021 Category: Allergy & Immunology Authors: Hanie Safarpour Mohammad Pourhassan-Moghaddam Adel Spotin Hassan Majdi Aleksandra Barac Mehdi Yousefi Ehsan Ahmadpour Source Type: research

A novel enhanced dot blot immunoassay using colorimetric biosensor for detection of Toxoplasma gondii infection
This study reports development of a novel point of care assay, namely an enhanced immuno-dot blot assay, for discrimination of anti-Toxoplasma IgG and anti-Toxoplasma IgM antibodies. This method has been designed based on formation of a sandwich complex between a gold nanoprobe (chitosan gold nanoparticle-anti-human IgG or anti-IgM) and anti- Toxoplasma lysate antigen (TLA) which holds anti-TLA antibodies, either IgG or IgM. Briefly, anti-human IgG or anti-IgM antibody was conjugated to chitosan gold nanoparticles via glutaraldehyde chemistry. Then, lysate antigen was immobilized on the surface of nitrocellulose membrane, ...
Source: Comparative immunology, microbiology and infectious diseases. - September 4, 2021 Category: Allergy & Immunology Authors: Hanie Safarpour Mohammad Pourhassan-Moghaddam Adel Spotin Hassan Majdi Aleksandra Barac Mehdi Yousefi Ehsan Ahmadpour Source Type: research

Molecular characterization and phylogenetic analysis of lumpy skin disease in Egypt
This study aimed to determine the genetic character of LSDV circulating in some governorates in Egypt and its relation with other LSDVs and vaccine strains in GenBank. A total of 50 skin nodules and 50 blood samples were collected from clinically affected cattle to detect LSDV using PCR targeting the P32 gene. The positive samples were characterized using PCR targeting the GPCR gene. The amplified products of four samples detected in the skin nodule of cattle from Alexandria and Kafr ElSheikh governorates were sequenced, and the phylogenetic tree was constructed. Out of 100 analyzed samples, 70 samples were positive for LS...
Source: Comparative immunology, microbiology and infectious diseases. - August 30, 2021 Category: Allergy & Immunology Authors: Abdelfattah Selim Eman Manaa Hanem Khater Source Type: research

Prevalence of leptospirosis among animal herds of north eastern provinces of India
In this study, the higher seroprevalence was observed in pigs (42.6 %), cattle (39.8 %) and goats (26 %) in NER. Cattle (36.4 %) and goat (17.6 %) showed higher prevalence in TN. The presence of rodents in pig herds was found to be significant (P = 0.0088) in NER and it was for cattle in TN (P = 0.0063). We suggest that a program of rodent control should be included in the flock management practices aiming to reduce transmission of the leptospires.PMID:34461344 | DOI:10.1016/j.cimid.2021.101698 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 30, 2021 Category: Allergy & Immunology Authors: Perumal Saranya Chandrani Goswami Kalimuthusamy Sumathi Alankar Hitlar Balasundareshwaran Palanisamy Bothammal Lakshya Jyoti Dutta Gangatharan Muralitharan Durlav Prasad Bora Kalimuthusamy Natarajaseenivasan Source Type: research

Multiresistant zoonotic pathogens isolated from goat milk in Northeastern Brazil
This study aimed to identify multiresistant zoonotic pathogens causing mastitis in goats, in addition to characterizing them for the presence of resistance genes and phenotypic resistance. A total of 714 milk samples from 357 lactating goats in 12 farms in the Northeast region of Brazil were analyzed. The isolates were submitted to Matrix Associated Laser Desorption-Ionization - Time of Flight to identify bacterial species, Polymerase Chain Reaction (PCR) to search for resistance genes, and an antibiogram to evaluate the phenotypic profile of antimicrobial resistance. A total of 214 pathogens were identified and bacterial ...
Source: Comparative immunology, microbiology and infectious diseases. - August 29, 2021 Category: Allergy & Immunology Authors: Breno Bezerra Arag ão Sabrina C ândido Trajano Raylson Pereira de Oliveira D ébora Mirelly Sobral da Silva Rafaella Grenfell de Carvalho Maria Aparecida Juliano Jos é Wilton Pinheiro Junior Rinaldo Aparecido Mota Source Type: research

Multiresistant zoonotic pathogens isolated from goat milk in Northeastern Brazil
This study aimed to identify multiresistant zoonotic pathogens causing mastitis in goats, in addition to characterizing them for the presence of resistance genes and phenotypic resistance. A total of 714 milk samples from 357 lactating goats in 12 farms in the Northeast region of Brazil were analyzed. The isolates were submitted to Matrix Associated Laser Desorption-Ionization - Time of Flight to identify bacterial species, Polymerase Chain Reaction (PCR) to search for resistance genes, and an antibiogram to evaluate the phenotypic profile of antimicrobial resistance. A total of 214 pathogens were identified and bacterial ...
Source: Comparative immunology, microbiology and infectious diseases. - August 29, 2021 Category: Allergy & Immunology Authors: Breno Bezerra Arag ão Sabrina C ândido Trajano Raylson Pereira de Oliveira D ébora Mirelly Sobral da Silva Rafaella Grenfell de Carvalho Maria Aparecida Juliano Jos é Wilton Pinheiro Junior Rinaldo Aparecido Mota Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India
In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10.7% by ELISA. Considerably higher prevalence was observed among milk samples (17.5%, n = 362) and in blood samples (37.7%, n = 262) by direct PCR. Clinical samples (n = 94) from late abortion cases yielded 11 B. abortus isolates. Multi-locus sequence typing indicated circulation of single sequence type, ST1. Whole genome sequencing (n = 8) and phylogenomic analysis reveale...
Source: Comparative immunology, microbiology and infectious diseases. - August 21, 2021 Category: Allergy & Immunology Authors: I Shakuntala A A P Milton R K Sanjukta Kaushik Kakoty Amarjit Karam Anirban Dutta K Puro Arnab Sen Samir Das Sandeep Ghatak Source Type: research

Phylogenetic analysis of kinetoplast DNA: kDNA of Leishmania tropica in Thi-Qar province, Iraq
CONCLUSION: The kDNA minicircle sequences analysis results showed mismatching of the local isolates decrease whenever approached from the Iranian border. In addition, genetic heterogeneity diagnosis is important for detection of therapy, control and epidemiological studies.PMID:34416483 | DOI:10.1016/j.cimid.2021.101696 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 20, 2021 Category: Allergy & Immunology Authors: Mohammed Hassan Flaih Fadhil Abbas Al-Abady Khwam Reissan Hussein Source Type: research

First molecular characterization of Blastocystis subtypes from animals and animal-keepers stool in Algeria
Comp Immunol Microbiol Infect Dis. 2021 Aug 12;78:101695. doi: 10.1016/j.cimid.2021.101695. Online ahead of print.ABSTRACTBlastocystis sp. is one of the most common enteric parasites found in humans and many non-human hosts. It is an anaerobic protozoan that belongs to the group of Stramenopiles. Based on phylogenetic analysis of ribosomal DNA genes, at least 17 subtypes (ST1-ST17) are described. The aim of this study was to identify and characterize Blastocystis sp. in stool samples from various animal groups and animal-keepers. Overall, 29/70 (41.43%) animals and 7/60 (11.66%) humans sampled were positive for Blastocysti...
Source: Comparative immunology, microbiology and infectious diseases. - August 20, 2021 Category: Allergy & Immunology Authors: Amina Boutellis Miriem Aissi Khaled Harhoura Rezak Drali Tahar Kernif Fadila Tazerouti Source Type: research

Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats
The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cult...
Source: Comparative immunology, microbiology and infectious diseases. - August 16, 2021 Category: Allergy & Immunology Authors: Juscil ânia Furtado Araújo Alice Andrioli Raymundo Rizaldo Pinheiro Renato Mesquita Peixoto Ana L ídia Madeira de Sousa Dalva Alana Arag ão de Azevedo Ana Milena Cesar Lima Juliana Ara újo Nobre Gabriel Paula Amaral Iane Sousa Brand ão Maria F átim Source Type: research

Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats
The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cult...
Source: Comparative immunology, microbiology and infectious diseases. - August 16, 2021 Category: Allergy & Immunology Authors: Juscil ânia Furtado Araújo Alice Andrioli Raymundo Rizaldo Pinheiro Renato Mesquita Peixoto Ana L ídia Madeira de Sousa Dalva Alana Arag ão de Azevedo Ana Milena Cesar Lima Juliana Ara újo Nobre Gabriel Paula Amaral Iane Sousa Brand ão Maria F átim Source Type: research

Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats
The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cult...
Source: Comparative immunology, microbiology and infectious diseases. - August 16, 2021 Category: Allergy & Immunology Authors: Juscil ânia Furtado Araújo Alice Andrioli Raymundo Rizaldo Pinheiro Renato Mesquita Peixoto Ana L ídia Madeira de Sousa Dalva Alana Arag ão de Azevedo Ana Milena Cesar Lima Juliana Ara újo Nobre Gabriel Paula Amaral Iane Sousa Brand ão Maria F átim Source Type: research

Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats
The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cult...
Source: Comparative immunology, microbiology and infectious diseases. - August 16, 2021 Category: Allergy & Immunology Authors: Juscil ânia Furtado Araújo Alice Andrioli Raymundo Rizaldo Pinheiro Renato Mesquita Peixoto Ana L ídia Madeira de Sousa Dalva Alana Arag ão de Azevedo Ana Milena Cesar Lima Juliana Ara újo Nobre Gabriel Paula Amaral Iane Sousa Brand ão Maria F átim Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
In conclusion, our results in bats from Montes Claros indicate that they do not act as hosts for Brucella spp. and Leptospira spp., although being potential carriers of Salmonella spp. in a low prevalence.PMID:34352638 | DOI:10.1016/j.cimid.2021.101692 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - August 5, 2021 Category: Allergy & Immunology Authors: Amanda Carvalho Rosado Ferreira Thallyta Maria Vieira Dirc éia Aparecida da Costa Custódio Maria Norma Melo C élia Maria Ferreira Gontijo Andrey Pereira Lage Elaine Maria Seles Dorneles Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique
CONCLUSION: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.PMID:34265544 | DOI:10.1016/j.cimid.2021.101690 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - July 15, 2021 Category: Allergy & Immunology Authors: Kavitha Manivannan Samar Mohamed Mahmoud Malathi Ramasamy Abeer A E Shehata Hanaa Ahmed Chandrasekar Solaimuthu Kaviyarasan Dhandapani Source Type: research