Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of T ürkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1
In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum-like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate ...
Source: Comparative immunology, microbiology and infectious diseases. - March 5, 2024 Category: Allergy & Immunology Authors: Ufuk Erol Omer Faruk Sahin Osman Furkan Urhan Ahmet Duran Atas Kursat Altay Source Type: research

Antimicrobial resistance and public and animal health risks associated with pathogenic Escherichia coli isolated from calves
Comp Immunol Microbiol Infect Dis. 2024 Feb 19;107:102149. doi: 10.1016/j.cimid.2024.102149. Online ahead of print.ABSTRACTWe aimed to determine the antimicrobial susceptibility profile of pathogenic Escherichia coli strains isolated from fecal samples of calves and buffalo calves (2008-2013), in Minas Gerais, Brazil, as well as the frequency of O157 gene and strains carrying extended-spectrum beta-lactamases (ESBL) and mobile colistin resistance (mcr) genes. E. coli strains (n=518) were tested for susceptibility against ten antimicrobials. Tetracycline was the antimicrobial with the highest resistance rate (382/518), foll...
Source: Comparative immunology, microbiology and infectious diseases. - March 5, 2024 Category: Allergy & Immunology Authors: Dirc éia Aparecida da Costa Custódio Carine Rodrigues Pereira Maysa Serpa Gon çalves Anna Cec ília Trolesi Reis Borges Costa Pedro Felipe Rodrigues de Oliveira Bruna Henrique Pinto da Silva Giovanna Botelho Carneiro Fernanda Morcatti Coura Andrey Pere Source Type: research

Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of T ürkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1
In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum-like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate ...
Source: Comparative immunology, microbiology and infectious diseases. - March 5, 2024 Category: Allergy & Immunology Authors: Ufuk Erol Omer Faruk Sahin Osman Furkan Urhan Ahmet Duran Atas Kursat Altay Source Type: research

Antimicrobial resistance and public and animal health risks associated with pathogenic Escherichia coli isolated from calves
Comp Immunol Microbiol Infect Dis. 2024 Feb 19;107:102149. doi: 10.1016/j.cimid.2024.102149. Online ahead of print.ABSTRACTWe aimed to determine the antimicrobial susceptibility profile of pathogenic Escherichia coli strains isolated from fecal samples of calves and buffalo calves (2008-2013), in Minas Gerais, Brazil, as well as the frequency of O157 gene and strains carrying extended-spectrum beta-lactamases (ESBL) and mobile colistin resistance (mcr) genes. E. coli strains (n=518) were tested for susceptibility against ten antimicrobials. Tetracycline was the antimicrobial with the highest resistance rate (382/518), foll...
Source: Comparative immunology, microbiology and infectious diseases. - March 5, 2024 Category: Allergy & Immunology Authors: Dirc éia Aparecida da Costa Custódio Carine Rodrigues Pereira Maysa Serpa Gon çalves Anna Cec ília Trolesi Reis Borges Costa Pedro Felipe Rodrigues de Oliveira Bruna Henrique Pinto da Silva Giovanna Botelho Carneiro Fernanda Morcatti Coura Andrey Pere Source Type: research

Clinical importance of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leishmaniasis
In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.PMID:38430666 | DOI:10.1016/j.cimid.2024.102148 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - March 2, 2024 Category: Allergy & Immunology Authors: A Dur án-Galea J I Crist óbal-Verdejo R Barrera-Chac ón B Mac ías-García M A Gonz ález-Solís P Nicol ás-Barceló A B Garc ía-Ibáñez P Ru íz-Tapia F J Duque-Carrasco Source Type: research

Clinical importance of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leishmaniasis
In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.PMID:38430666 | DOI:10.1016/j.cimid.2024.102148 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - March 2, 2024 Category: Allergy & Immunology Authors: A Dur án-Galea J I Crist óbal-Verdejo R Barrera-Chac ón B Mac ías-García M A Gonz ález-Solís P Nicol ás-Barceló A B Garc ía-Ibáñez P Ru íz-Tapia F J Duque-Carrasco Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102151. doi: 10.1016/j.cimid.2024.102151. Online ahead of print.ABSTRACTHemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in N...
Source: Comparative immunology, microbiology and infectious diseases. - February 22, 2024 Category: Allergy & Immunology Authors: Javier Mill án B árbara Martín-Maldonado Ruth Rodr íguez-Pastor Jes ús Martínez-Padilla Fernando Esper ón Source Type: research

High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102151. doi: 10.1016/j.cimid.2024.102151. Online ahead of print.ABSTRACTHemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in N...
Source: Comparative immunology, microbiology and infectious diseases. - February 22, 2024 Category: Allergy & Immunology Authors: Javier Mill án B árbara Martín-Maldonado Ruth Rodr íguez-Pastor Jes ús Martínez-Padilla Fernando Esper ón Source Type: research