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Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9...
Source: Comparative immunology, microbiology and infectious diseases. - February 24, 2024 Category: Allergy & Immunology Authors: Paulina Sep úlveda-García Ronald Jara Armin Mella Gustavo Monti Nivia Canales Maria Eduarda Chiaradia Furquim Marcos Rog ério André Ananda M üller Source Type: research

High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals
Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102151. doi: 10.1016/j.cimid.2024.102151. Online ahead of print.ABSTRACTHemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in N...
Source: Comparative immunology, microbiology and infectious diseases. - February 22, 2024 Category: Allergy & Immunology Authors: Javier Mill án B árbara Martín-Maldonado Ruth Rodr íguez-Pastor Jes ús Martínez-Padilla Fernando Esper ón Source Type: research

Investigation of anti-Leptospira spp. antibodies and leptospiruria in cats attended to a veterinary teaching hospital in southern Brazil
This study aims to investigate the presence of anti-Leptospira spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-Leptospira spp. antibodies, while Leptospira spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecul...
Source: Comparative immunology, microbiology and infectious diseases. - February 17, 2024 Category: Allergy & Immunology Authors: Mirela Gr ünwalder Paim B árbara Basseggio Rivas Grasiele Amorim Sebasti ão Kauana Kaefer Rog ério Oliveira Rodrigues Fabiana Quoos Mayer Luciana Neves Nunes Fernanda Vieira Amorim da Costa Source Type: research

Prevalence and potential risk factors for Cryptosporidium spp. infection in horses from Egypt
Comp Immunol Microbiol Infect Dis. 2024 Feb 9;106:102140. doi: 10.1016/j.cimid.2024.102140. Online ahead of print.ABSTRACTCryptosporidium is an intestinal protozoan that cause diarrhea in livestock all over the world and have zoonotic importance. The present study aimed to determine the prevalence of Cryptosporidium spp. in horses in Egypt and evaluate the associated risk factors. A total of 420 fecal samples were collected from three governorates (Giza, Kafr ElSheikh and Qalyubia) and examined microscopically using Ziehl-Neelsen staining method. The overall prevalence of Cryptosporidium spp. was 29% and Kafr ElSheikh gove...
Source: Comparative immunology, microbiology and infectious diseases. - February 13, 2024 Category: Allergy & Immunology Authors: Mohammed H Alruhaili Mohamed Marzok Hattan S Gattan Mohamed Salem Mahmoud Kandeel Abdelfattah Selim Source Type: research

Molecular prevalence of vector borne bacterial pathogens in the blood samples of wild rodent species trapped from Saudi Arabia
In conclusion, we are reporting for the very first time that Saudi rodents are infected with Anaplasma ovis and rodents can be infected with Bartonella koehlerae. Similar studies at large scale are recommended in all those areas of Saudi Arabia that are unexplored for the incidence and prevalence of bacterial pathogens among the rodents that are living near human dwellings in order to prevent bacterial infections in local people as well as in livestock.PMID:38335834 | DOI:10.1016/j.cimid.2024.102129 (Source: Comparative immunology, microbiology and infectious diseases.)
Source: Comparative immunology, microbiology and infectious diseases. - February 9, 2024 Category: Allergy & Immunology Authors: Abdul Ghafar Samia Q Alghamdi Abdullah D Alanazi Syed Muhammad Zukhruf Qousain Maryam Ijaz Muhammad Naeem Muhammad Ali Hira Muqaddas Adil Khan Furhan Iqbal Source Type: research

Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria
Comp Immunol Microbiol Infect Dis. 2024 Jan 24;106:102126. doi: 10.1016/j.cimid.2024.102126. Online ahead of print.ABSTRACTAiming at identifying the reservoir and contamination sources of Coxiella burnetii in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that 15/344 (4.36%) blood samples and six/255 (2.35%) organ specimens were positive for C. burnetii. In cattle, three (4%) blood and liver samples were positive. In sheep, one blood (1.19%) and 3 (8.57%) placenta sampl...
Source: Comparative immunology, microbiology and infectious diseases. - February 7, 2024 Category: Allergy & Immunology Authors: H Ghaoui I Bitam S Zaidi N Achour S Zenia T Idres P E Fournier Source Type: research

Beta-lactamase genes in bacteria from food animals, retail meat, and human surveillance programs in the United States from 2002 to 2021
This study aimed to explore the distribution of beta-lactamases reported in three sampling sources (cecal, retail meat, and human) collected as part of integrated surveillance in the United States. We retrieved and analyzed data from the United States National Antimicrobial Resistance Monitoring Systems (NARMS) from 2002 to 2021. A total of 115 beta-lactamase genes were detected in E. coli, Salmonella enterica, Campylobacter, Shigella and Vibrio: including 35 genes from cecal isolates, 32 genes from the retail meat isolates, and 104 genes from the human isolates. Three genes in E. coli (blaCMY-2,blaTEM-1A, and blaTEM-1B), ...
Source: Comparative immunology, microbiology and infectious diseases. - February 7, 2024 Category: Allergy & Immunology Authors: Md Kaisar Rahman Howard Rodriguez-Mori Guy H Loneragan Babafela Awosile Source Type: research